CN1185274A - Method for selective breeding of wheet seeds resisting wheat chlorisis - Google Patents
Method for selective breeding of wheet seeds resisting wheat chlorisis Download PDFInfo
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- CN1185274A CN1185274A CN96114373A CN96114373A CN1185274A CN 1185274 A CN1185274 A CN 1185274A CN 96114373 A CN96114373 A CN 96114373A CN 96114373 A CN96114373 A CN 96114373A CN 1185274 A CN1185274 A CN 1185274A
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Abstract
The new germplasm Y 96064 of wheat capable of resisting Verticillium wilt virus of wheat (BYDV) is a general wheat-intermediary quack grass translocation line obtained by means of sexual hybridization and embryo culture technique. It carries the gene which is present in intermediary quack grass 2Ai-2 chromosome and possesses the function of resisting Verticillium wilt of wheat, and its resistance is superior to the resistance gene from intermediary quack grass 7Ai-1 chromosome, so that it possesses high resistance to Verticillium wilt virus GPV and GAV strain lines of Chinese wheat and international PAV strain line. Said disease-resisting translocation line Y96064 can be confirmed by means of cytogenetic analysis, chromosome C zoning and in situ hybridization and RAPD techniques.
Description
The present invention is the selection of a kind of anti-yellow stunt of wheat viral disease (BYDV) wheat germplasm, belongs to the biotechnology breeding research field.
Yellow stunt of wheat is the virus disease that is caused by luteovirus (Barley Yellow Dwarf Virus is abbreviated as BYDV), and many in the world wheat main products state often takes a bath agricultural because of the harm of yellow dwarf.According to the U.S. and Australia, the annual evil loss of therefore being critically ill is about 9,500 ten thousand dollars and 3,000 ten thousand dollars; The annual therefore disease underproduction 5-20% of 1976-1993 Chile.NORTHWEST CHINA, northeast and some areas, North China often suffer this evil of being critically ill, serious time underproduction 20-30%.In China spreading trend is arranged in recent years, the harm area expands to the Yellow River and Huai He River, Sichuan, middle and lower reach of Yangtze River Mai Qu.Wheat breeding and production practices prove that most economical, the effective method of preventing and treating the yellow stunt of wheat viral disease is seed selection, popularization disease-resistant variety.Since early fifties begins this disease studied, through extensively identifying, in Triticum, do not find the anti-source of BYDV as yet, the minority disease tolerant variety is only arranged, be difficult to prove effective so utilize in the Triticum genetic resources to carry out the BYDV resistive breeding.The eighties begins that wheat sibling species genus is carried out the BYDV resistance and identifies, find that tens kinds of wild plants such as couchgrass genus, Agropyron, leymus, goose hat grass genus have the BYDV resistant gene. these genes can suppress the BYDV breeding, thereby to BYDV performance immunity or high anti-.According to research reports, have at least two couples of chromosome 7Ai-1 and 2Ai-2 to contain anti-BYDV gene in the middle couchgrass, and the disease-resistant gene on the 2Ai-2 have stronger resistance than the disease-resistant gene on the 7Ai-1.
Shift the external source resistant gene to common wheat and remove conventional sexual hybridization method, utilize the CSph cryptic mutant, outside the chromosome engineering methods such as wheat list nullisomic, developed the biological technique method-tissue culture that forms since the eighties in addition.The anti-BYDV gene that how middle couchgrass is carried at present imports to common wheat, and the common wheat new germ plasm of creating the first in the world anti-BYDV virus disease is the target that the breeding for disease resistance man pursues always.
The objective of the invention is to adopt the method for sexual hybridization and biotechnology, with come from the no awns 4, resistance is better than that 7Ai-1 chromosome carries, the resistant gene that is positioned on the 2Ai-2 chromosome imports wheat, realize the variation of anti-source, enlarge the anti-spectrum of wheat breed, it is single because of anti-source to remedy wheat breed, and resistance is the hidden danger of forfeiture easily.And the further sound technology path that utilizes tissue culture technique to import the external source desirable genes to wheat.
Another object of the present invention is susceptible variety and the hybrid that carries the allos disomic addition line of disease-resistant gene to be carried out rataria cultivate, and by the chromosomal break in the incubation, reclosing and reorganization, creates alien translocation line disease-resistant, genetic stability.The external source chromatin that translocation line contains can divide band and RAPD analysis to differentiate by Chromosomal in situ hybridization, C.
A kind of selection of wheat seeds resisting wheat chlorisis is with 7902 not having in the awns wheat-middle couchgrass allos disomic addition line Z of 4 hybridization, the anti-yellow stunt of wheat viral disease of backcross breeding with wheat-middle couchgrass partial amphidiploid in the common wheat
1Use Z
1With Anhui 91808 wheat hybridizings, the hybrid rataria carries out tissue culture, by chromosomal break and reorganization, and the wheat of the anti-yellow stunt of wheat viral disease of seed selection-middle couchgrass translocation line Y96064.
Disease-resistant translocation line Y96064 is the transposition of chromosome end, and chromosome number is 2n=42=21II, and genetic stability is good, economical character is good, is beneficial to the breeding for disease resistance man and utilizes.
Disease-resistant translocation line Y96064 can resist the wheat yellow dwarf virus GPV of China and GAV strain to be, also anti-international PAV strain is.
Disease-resistant translocation line Y96064 identifies that through genetics of resistance its resistant gene is a dominant inheritance, and is disease-resistant with susceptible variety hybridization F1 performance.
The chromosome of disease-resistant translocation line Y96064 constitutes, and can divide band, in situ hybridization and RAPD technology to obtain confirming by CYTOGENETIC ANALYSIS OF ONE chromosome C.
Advantage of the present invention and extremely long-pending effect are:
1. realize the variation of anti-source
Prove that through the inoculation approval test translocation line Y96064 that the present invention obtains is to the wheat yellow dwarf virus GPV of China, GAV strain system, all performance is high anti-to international PAV strain system, is the best common wheat new germ plasm of anti-BYDV in having appeared in the newspapers both at home and abroad at present.
2. translocation line Y96064 of the present invention is better than the disease-resistant germplasm resistance with the anti-BYDV gene of 7Ai-1 chromosome of seed selection.
3. translocation line Y96064 of the present invention is the transposition of chromosome end, and middle couchgrass chromatin content is few, and genetic background is the recommended variety of Chinese wheat main producing region, and economical character is better, is beneficial to the breeding for disease resistance man and utilizes.
4. the tissue culture technique that seed selection process of the present invention adopts is quick, easy.
Fig. 1 is the seed selection programme diagram of translocation line Y96064 of the present invention
Narrate embodiments of the invention below in conjunction with accompanying drawing:
The selection of a kind of wheat seeds resisting wheat chlorisis Y96064 of the present invention may further comprise the steps:
7902 do not have in the awns wheat-middle couchgrass allos disomic addition line Z of 4 hybridization, the anti-yellow stunt of wheat viral disease of backcross breeding with wheat-middle couchgrass partial amphidiploid in the 1 usefulness common wheat
1
2 use Z
1With Anhui 91808 wheat hybridizings, the hybrid rataria carries out tissue culture
By chromosomal break and reorganization, the wheat of the anti-yellow stunt of wheat viral disease of seed selection--middle couchgrass translocation line
1. 92 ratarias are at SD
2On the medium, evoked callus
2. nascent callus is at SC
1Successive transfer culture is 2 times on the medium, the green seedling of differentiation on the 1/2MS medium.
3. obtain regeneration plant 250 strains (on average each rataria regenerate 2.72 strains), transplant survival 229 strains, transplanting survival rate 91.6%.
3 SC
1Identify and selection for resistance
1. poison (BYDV GPV strain system) aphid is raised in the field inoculation, observes the record disease resistance after 40 days, records disease-resistant 186 strains, susceptible 43 strains.
2. select disease-resistant and economical character individual plant 21 strains preferably (code T9418)
3. select for use susceptible variety capital 411 to carry out test cross (T9418 * capital 411)
4 SC
2Identify and selection for resistance
1. plant T9418 selfing strain nursery and test cross combination (T9418 * capital 411)
2. poison (BYDV GAV strain system) aphid is raised in the field inoculation, observes the record disease resistance after 40 days,
Selfing strain nursery T9418 records disease-resistant 112 strains, and susceptible 32 strains are anti-: the sense ratio was greater than 3: 1
Test cross combination T9418 * capital 411 records disease-resistant 14 strains, and susceptible 11 strains are anti-: the sense ratio was greater than 1: 1
3. select anti-: feel plant-Y96064 greater than 3: 1
The cytology of 5 disease-resistant translocation line Y96064 and molecular biology identification
Disease-resistant translocation line Y96064 pollen mother cell chromosomes number is 21II.Root-tip cells chromosome is blockaded with the common wheat genomic DNA, and biotin labeled middle couchgrass genomic DNA carries out in situ hybridization as probe, shows that there is middle couchgrass chromosome segment the dyad end.
The economical character of 6 disease-resistant translocation line Y96064
Weak winter habit, long awns, white shell, red grain, in high stalk, in ripe.
Claims (5)
1. the selection of a wheat seeds resisting wheat chlorisis comprises the wheat-middle couchgrass allos disomic addition line Z that does not have 4 hybridization, the anti-yellow stunt of wheat viral disease of backcross breeding in the awns with common wheat 7902 and wheat-middle couchgrass partial amphidiploid
1, it is characterized in that: use Z
1With Anhui 91808 wheat hybridizings, the hybrid rataria carries out tissue culture, by chromosomal break and reorganization, and the wheat of the anti-yellow stunt of wheat viral disease of seed selection-middle couchgrass translocation line Y96064.
2. selection according to claim 1 is characterized in that: disease-resistant translocation line Y96064, be the transposition of chromosome end, and chromosome number is 2n=42=21II, genetic stability is good, economical character is good, is beneficial to the breeding for disease resistance man and utilizes.
3. selection according to claim 1 is characterized in that: disease-resistant translocation line Y96064, and can resist the wheat yellow dwarf virus GPV of China and GAV strain to be, also anti-international PAV strain is.
4. selection according to claim 1 is characterized in that: disease-resistant translocation line Y96064 is through Genetic identification, and its resistant gene is a dominant inheritance, and is disease-resistant with susceptible variety hybridization F1 performance.
5. selection according to claim 1 is characterized in that: the chromosome of disease-resistant translocation line Y96064 constitutes, and can divide band, in situ hybridization and RAPD technology to obtain confirming by CYTOGENETIC ANALYSIS OF ONE chromosome C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96114373A CN1063011C (en) | 1996-12-17 | 1996-12-17 | Method for selective breeding of wheet seeds resisting wheat chlorisis |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN96114373A CN1063011C (en) | 1996-12-17 | 1996-12-17 | Method for selective breeding of wheet seeds resisting wheat chlorisis |
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| Publication Number | Publication Date |
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| CN1185274A true CN1185274A (en) | 1998-06-24 |
| CN1063011C CN1063011C (en) | 2001-03-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN96114373A Expired - Fee Related CN1063011C (en) | 1996-12-17 | 1996-12-17 | Method for selective breeding of wheet seeds resisting wheat chlorisis |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100354419C (en) * | 2003-07-25 | 2007-12-12 | 中国农业科学院作物育种栽培研究所 | Wheat yellow dwarf disease candidate resistance gene and its coded protein and uses |
| CN100372934C (en) * | 2003-07-10 | 2008-03-05 | 中国农业科学院作物育种栽培研究所 | Homologous sequence linked with yellow dwarf-resistant gene Bdv2 and use thereof |
| CN108315337A (en) * | 2018-02-09 | 2018-07-24 | 中国农业科学院植物保护研究所 | Wheat Yellow strip virus and its NASH detection methods, detection kit |
| CN110643731A (en) * | 2019-10-30 | 2020-01-03 | 山西省农业科学院作物科学研究所 | Rapid detection of thinopyrum intermedium 6JSSpecific molecular marker of chromosome and application |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1065109C (en) * | 1996-12-17 | 2001-05-02 | 中国农业科学院作物育种栽培研究所 | Method for breeding of wheat seeds resisting wheat chlorisis |
-
1996
- 1996-12-17 CN CN96114373A patent/CN1063011C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100372934C (en) * | 2003-07-10 | 2008-03-05 | 中国农业科学院作物育种栽培研究所 | Homologous sequence linked with yellow dwarf-resistant gene Bdv2 and use thereof |
| CN100354419C (en) * | 2003-07-25 | 2007-12-12 | 中国农业科学院作物育种栽培研究所 | Wheat yellow dwarf disease candidate resistance gene and its coded protein and uses |
| CN108315337A (en) * | 2018-02-09 | 2018-07-24 | 中国农业科学院植物保护研究所 | Wheat Yellow strip virus and its NASH detection methods, detection kit |
| CN108315337B (en) * | 2018-02-09 | 2021-06-25 | 中国农业科学院植物保护研究所 | Wheat yellow stripe virus and NASH detection method and detection kit thereof |
| CN110643731A (en) * | 2019-10-30 | 2020-01-03 | 山西省农业科学院作物科学研究所 | Rapid detection of thinopyrum intermedium 6JSSpecific molecular marker of chromosome and application |
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| Publication number | Publication date |
|---|---|
| CN1063011C (en) | 2001-03-14 |
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