CN116283418B - Plant growth promoter immobilized with microorganism and preparation method thereof - Google Patents
Plant growth promoter immobilized with microorganism and preparation method thereof Download PDFInfo
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- CN116283418B CN116283418B CN202310595419.5A CN202310595419A CN116283418B CN 116283418 B CN116283418 B CN 116283418B CN 202310595419 A CN202310595419 A CN 202310595419A CN 116283418 B CN116283418 B CN 116283418B
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- polyvinyl alcohol
- plant growth
- immobilized
- growth promoter
- modified polyvinyl
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- 244000005700 microbiome Species 0.000 title claims abstract description 80
- 230000008635 plant growth Effects 0.000 title claims abstract description 57
- 239000007952 growth promoter Substances 0.000 title claims abstract description 52
- 238000002360 preparation method Methods 0.000 title claims abstract description 29
- 239000004372 Polyvinyl alcohol Substances 0.000 claims abstract description 93
- 229920002451 polyvinyl alcohol Polymers 0.000 claims abstract description 93
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 32
- 239000000661 sodium alginate Substances 0.000 claims abstract description 32
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 32
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 32
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 20
- 240000006024 Lactobacillus plantarum Species 0.000 claims abstract description 20
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims abstract description 20
- 229940072205 lactobacillus plantarum Drugs 0.000 claims abstract description 20
- 241000193388 Bacillus thuringiensis Species 0.000 claims abstract description 19
- 229940097012 bacillus thuringiensis Drugs 0.000 claims abstract description 19
- 239000002131 composite material Substances 0.000 claims abstract description 19
- 238000002156 mixing Methods 0.000 claims abstract description 17
- 239000003895 organic fertilizer Substances 0.000 claims abstract description 17
- 238000000576 coating method Methods 0.000 claims abstract description 15
- 239000011248 coating agent Substances 0.000 claims abstract description 14
- 238000003756 stirring Methods 0.000 claims abstract description 13
- 229920002197 Sodium polyaspartate Polymers 0.000 claims abstract description 12
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000001768 carboxy methyl cellulose Substances 0.000 claims abstract description 10
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 claims abstract description 10
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 claims abstract description 10
- 238000001035 drying Methods 0.000 claims abstract description 7
- 239000000243 solution Substances 0.000 claims description 30
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 27
- ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 claims description 22
- 239000007864 aqueous solution Substances 0.000 claims description 20
- 238000000034 method Methods 0.000 claims description 20
- PFKFTWBEEFSNDU-UHFFFAOYSA-N carbonyldiimidazole Chemical compound C1=CN=CN1C(=O)N1C=CN=C1 PFKFTWBEEFSNDU-UHFFFAOYSA-N 0.000 claims description 19
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 16
- 229940125904 compound 1 Drugs 0.000 claims description 15
- 230000001580 bacterial effect Effects 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 12
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims description 11
- 238000006243 chemical reaction Methods 0.000 claims description 11
- 238000010438 heat treatment Methods 0.000 claims description 11
- 229940125782 compound 2 Drugs 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 108010059892 Cellulase Proteins 0.000 claims description 8
- PASDCCFISLVPSO-UHFFFAOYSA-N benzoyl chloride Chemical compound ClC(=O)C1=CC=CC=C1 PASDCCFISLVPSO-UHFFFAOYSA-N 0.000 claims description 8
- 229940106157 cellulase Drugs 0.000 claims description 8
- 238000000874 microwave-assisted extraction Methods 0.000 claims description 8
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 8
- 229920000053 polysorbate 80 Polymers 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 7
- UKVIEHSSVKSQBA-UHFFFAOYSA-N methane;palladium Chemical compound C.[Pd] UKVIEHSSVKSQBA-UHFFFAOYSA-N 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 6
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical class OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 6
- 238000001914 filtration Methods 0.000 claims description 6
- 230000002829 reductive effect Effects 0.000 claims description 6
- 239000008223 sterile water Substances 0.000 claims description 6
- 238000005406 washing Methods 0.000 claims description 6
- 239000001110 calcium chloride Substances 0.000 claims description 5
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 5
- 239000000706 filtrate Substances 0.000 claims description 5
- 238000004132 cross linking Methods 0.000 claims description 4
- 238000001704 evaporation Methods 0.000 claims description 4
- -1 rawhide Substances 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 235000019733 Fish meal Nutrition 0.000 claims description 3
- 239000004467 fishmeal Substances 0.000 claims description 3
- 229910052739 hydrogen Inorganic materials 0.000 claims description 3
- 239000001257 hydrogen Substances 0.000 claims description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 2
- 239000002374 bone meal Substances 0.000 claims description 2
- 229940036811 bone meal Drugs 0.000 claims description 2
- 235000019779 Rapeseed Meal Nutrition 0.000 claims 1
- 235000019764 Soybean Meal Nutrition 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 claims 1
- AICOOMRHRUFYCM-ZRRPKQBOSA-N oxazine, 1 Chemical compound C([C@@H]1[C@H](C(C[C@]2(C)[C@@H]([C@H](C)N(C)C)[C@H](O)C[C@]21C)=O)CC1=CC2)C[C@H]1[C@@]1(C)[C@H]2N=C(C(C)C)OC1 AICOOMRHRUFYCM-ZRRPKQBOSA-N 0.000 claims 1
- 239000004456 rapeseed meal Substances 0.000 claims 1
- 239000004455 soybean meal Substances 0.000 claims 1
- 239000003337 fertilizer Substances 0.000 abstract description 18
- 239000002689 soil Substances 0.000 abstract description 18
- 230000000694 effects Effects 0.000 abstract description 15
- 230000035558 fertility Effects 0.000 abstract description 6
- 239000000969 carrier Substances 0.000 abstract description 4
- 241000894006 Bacteria Species 0.000 abstract description 3
- 230000000813 microbial effect Effects 0.000 description 19
- 230000000052 comparative effect Effects 0.000 description 10
- 235000013399 edible fruits Nutrition 0.000 description 9
- 238000012360 testing method Methods 0.000 description 8
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 7
- 240000003768 Solanum lycopersicum Species 0.000 description 7
- 230000003100 immobilizing effect Effects 0.000 description 7
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 6
- 150000001875 compounds Chemical class 0.000 description 6
- 239000003795 chemical substances by application Substances 0.000 description 5
- 230000004720 fertilization Effects 0.000 description 5
- 125000001997 phenyl group Chemical group [H]C1=C([H])C([H])=C(*)C([H])=C1[H] 0.000 description 5
- IKMXRUOZUUKSON-UHFFFAOYSA-N 2-(4-nitrophenyl)ethanol Chemical compound OCCC1=CC=C([N+]([O-])=O)C=C1 IKMXRUOZUUKSON-UHFFFAOYSA-N 0.000 description 4
- 150000001408 amides Chemical group 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 230000012010 growth Effects 0.000 description 4
- 230000008569 process Effects 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- XEKOWRVHYACXOJ-UHFFFAOYSA-N Ethyl acetate Chemical compound CCOC(C)=O XEKOWRVHYACXOJ-UHFFFAOYSA-N 0.000 description 3
- KDLHZDBZIXYQEI-UHFFFAOYSA-N Palladium Chemical compound [Pd] KDLHZDBZIXYQEI-UHFFFAOYSA-N 0.000 description 3
- 239000004743 Polypropylene Substances 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 125000004185 ester group Chemical group 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 229920001155 polypropylene Polymers 0.000 description 3
- 229920002635 polyurethane Polymers 0.000 description 3
- 239000004814 polyurethane Substances 0.000 description 3
- 238000005160 1H NMR spectroscopy Methods 0.000 description 2
- KDCGOANMDULRCW-UHFFFAOYSA-N 7H-purine Chemical compound N1=CNC2=NC=NC2=C1 KDCGOANMDULRCW-UHFFFAOYSA-N 0.000 description 2
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000004820 blood count Methods 0.000 description 2
- 230000009920 chelation Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000005469 granulation Methods 0.000 description 2
- 230000003179 granulation Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229920000642 polymer Polymers 0.000 description 2
- 239000011591 potassium Substances 0.000 description 2
- 229910052700 potassium Inorganic materials 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 240000007087 Apium graveolens Species 0.000 description 1
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
- 235000010591 Appio Nutrition 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 235000000832 Ayote Nutrition 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 240000004244 Cucurbita moschata Species 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000088415 Raphanus sativus Species 0.000 description 1
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000004327 boric acid Substances 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 229910021393 carbon nanotube Inorganic materials 0.000 description 1
- 239000002041 carbon nanotube Substances 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 239000003610 charcoal Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000006482 condensation reaction Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000005336 cracking Methods 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000002542 deteriorative effect Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 238000000921 elemental analysis Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229910002804 graphite Inorganic materials 0.000 description 1
- 239000010439 graphite Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 150000002431 hydrogen Chemical class 0.000 description 1
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001546 nitrifying effect Effects 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000012074 organic phase Substances 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- DOKHEARVIDLSFF-UHFFFAOYSA-N prop-1-en-1-ol Chemical class CC=CO DOKHEARVIDLSFF-UHFFFAOYSA-N 0.000 description 1
- 235000015136 pumpkin Nutrition 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08F—MACROMOLECULAR COMPOUNDS OBTAINED BY REACTIONS ONLY INVOLVING CARBON-TO-CARBON UNSATURATED BONDS
- C08F8/00—Chemical modification by after-treatment
- C08F8/30—Introducing nitrogen atoms or nitrogen-containing groups
- C08F8/32—Introducing nitrogen atoms or nitrogen-containing groups by reaction with amines
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/40—Mixtures of one or more fertilisers with additives not having a specially fertilising activity for affecting fertiliser dosage or release rate; for affecting solubility
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/10—Solid or semi-solid fertilisers, e.g. powders
- C05G5/12—Granules or flakes
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/30—Layered or coated, e.g. dust-preventing coatings
- C05G5/37—Layered or coated, e.g. dust-preventing coatings layered or coated with a polymer
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- General Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Plant Pathology (AREA)
- Fertilizers (AREA)
Abstract
The application belongs to the technical field of agricultural fertilizers, and particularly relates to a plant growth promoter immobilized with microorganisms and a preparation method thereof. Firstly, using modified polyvinyl alcohol and sodium alginate as carriers to fix composite bacteria of lactobacillus plantarum, bacillus licheniformis and bacillus thuringiensis so as to obtain immobilized microorganisms; then adding sodium carboxymethyl cellulose, sodium polyaspartate and organic fertilizer into the gulfweed extract, stirring, mixing, adding immobilized microorganism, mixing, granulating, coating with modified polyvinyl alcohol, and drying to obtain plant growth promoter. The plant growth promoter prepared by the application has good slow release effect, can slowly release the effective components such as organic fertilizer, gulfweed extract, microorganism and the like, so that the soil can keep high fertility for a long time, the plant growth is continuously promoted, and the yield is improved; and is suitable for various crops.
Description
Technical Field
The application belongs to the technical field of agricultural fertilizers, and particularly relates to a plant growth promoter immobilized with microorganisms and a preparation method thereof.
Background
The fertilizer is a material foundation for maintaining and improving the soil fertility and realizing sustainable development of agriculture. The microbial fertilizer is a microbial agent prepared from one or more beneficial living microorganisms, and the microbial agent is used for improving plant growth conditions by means of metabolic processes or metabolic products of the microbial agent, so that the effect of promoting plant growth is achieved, and the aim of keeping microbial activity is key for preparing the microbial and compound microbial fertilizer. The compound microbial fertilizer is formed by compounding specific microorganisms with a traditional fertilizer. The compound microbial fertilizer can improve the physicochemical property of soil, improve the quick-acting fertility of the soil, inhibit the occurrence of soil-borne diseases, promote the growth of crops and has important significance for improving the yield and the sustainable development of agriculture.
In the existing preparation process of the compound microbial fertilizer, one of the adding modes of the microorganism is mixing and granulating of strains and the traditional fertilizer, and the process of adding the microorganism in the granulating process can cause massive death of the microorganism due to factors such as high temperature, high salt, drying and the like, and particularly, the high nitrogen in the inorganic fertilizer has extremely strong killing power on the microorganism. The application number 202110855076.2 discloses a special microbial compound fertilizer for vegetables and a preparation method thereof, wherein the microbial compound fertilizer is prepared by inoculating a composite liquid microbial agent into a sterilized carrier containing flower soil, potato residues, turf and charcoal, and the components of an organic fertilizer carrier are not chemically killed to microbial bacteria, but the composite liquid microbial agent is directly mixed with the organic fertilizer carrier, so that the activity of microorganisms after long-term storage is difficult to ensure.
The immobilized microorganism technology is considered as one of the most promising treatment methods because of being capable of weakening the toxicity of the environment to microorganisms and maintaining the activity of the microorganisms; the carrier of the immobilized microorganism is the basis of the implementation of the immobilized microorganism technology, and provides a place for the microorganism to grow and attach and react; the biochar carrier (such as active carbon, carbon nano tube, graphite and the like) has the advantages of high pore space, large specific surface area, good stability, no toxicity, no pollution and the like, but has fewer surface functional groups, does not contain hydrophilic groups (-COOH, -OH), and is difficult to combine with other substances through chemical action force when being singly used for fixing microorganisms. The literature [ study on preparation of polyvinyl alcohol/sodium alginate/aqueous polyurethane composite carrier and degradation of ammonia nitrogen wastewater by immobilized nitrifying bacteria [ J ]. Water treatment technology, 2022, 48 (11): 94-97.] reports that the composite carrier with a porous structure, which has good chemical stability and is suitable for survival and propagation of microorganisms, is successfully prepared from polyvinyl alcohol, sodium alginate and aqueous polyurethane, wherein the aqueous polyurethane has poor film forming property, and the polyvinyl alcohol has high self hydrophilicity due to an alkane linear molecular structure, and the mechanical property and thermal stability of the polyvinyl alcohol are also to be improved. Therefore, the research on the immobilized microorganism carrier with good comprehensive performance plays a vital role in further preparing the immobilized microorganism fertilizer.
Disclosure of Invention
In order to solve the problems, the application provides a plant growth promoter for immobilizing microorganisms and a preparation method thereof, wherein modified polyvinyl alcohol and sodium alginate are used as carriers for immobilizing microorganisms, so that the activity of the microorganisms can be maintained, more microorganisms can be continuously released into soil to play a role, and the bioavailability of the microorganisms is improved; the plant growth promoter coated by the modified polyvinyl alcohol has good slow release effect, so that the soil can keep high fertility for a long time, and plant growth is continuously promoted; the plant growth promoter prepared by the application is suitable for various crops, can effectively improve the yield, and can meet the market demand.
The technical scheme for solving the problems is as follows:
a preparation method of a plant growth promoter immobilized with microorganisms comprises the following steps:
s1, uniformly mixing an aqueous solution of modified polyvinyl alcohol and sodium alginate with a composite bacterial solution containing lactobacillus plantarum, bacillus licheniformis and bacillus thuringiensis according to a mass ratio of 1:0.8-1, dripping the mixture into a saturated boric acid solution containing 1-2% of calcium chloride by using a syringe, crosslinking and curing the mixture for 2-4 hours, and washing the mixture with sterile water for 2 times to obtain immobilized microorganisms;
s2, crushing gulfweed, sieving with a 40-70 mesh sieve to obtain gulfweed powder, sequentially adding the gulfweed powder, cellulase and Tween 80 into a 75-85% ethanol solution, soaking for 20-30min, placing into a microwave extractor, extracting for 4-6min by microwaves, filtering, and evaporating ethanol from filtrate under reduced pressure to obtain a gulfweed extract;
and S3, sequentially adding sodium carboxymethyl cellulose, sodium polyaspartate and organic fertilizer into the gulfweed extract, stirring and mixing uniformly, adding immobilized microorganisms, mixing uniformly, granulating, coating by modified polyvinyl alcohol, and drying to obtain the plant growth promoter.
The preparation method of the modified polyvinyl alcohol comprises the following steps: adding polyvinyl alcohol into dimethyl sulfoxide solution, heating to 95 ℃, stirring until the polyvinyl alcohol is dissolved, naturally cooling to room temperature, adding N, N' -carbonyl diimidazole into the reaction solution, continuously reacting for 2-4h, adding compound 1, heating to 50-60 ℃, and reacting for 8-10h to obtain modified polypropylene alcohol; the mass ratio of the polyvinyl alcohol to the N, N' -carbonyl diimidazole to the compound 1 is 1:1.1-1.2:0.6-0.8; the reaction process is as follows:
。
further, the preparation method of the compound 1 comprises the following steps:
(1) Adding p-nitrophenethyl alcohol and triethylamine into an anhydrous N, N-dimethylformamide solution, slowly dropwise adding benzoyl chloride, heating to 40-50 ℃ after dropwise adding, and reacting for 2-3h to obtain a compound 2, wherein the molar ratio of the p-nitrophenethyl alcohol to the benzoyl chloride to the triethylamine is 1:1.5-1.8:1.5-2.0; the reaction process is as follows:
；
adding the compound 2 and palladium carbon into a methanol solution, introducing hydrogen with the pressure of 0.1 MPa, and reacting for 6-8 hours at room temperature to obtain a compound 1; the mass ratio of the compound 2 to the palladium-carbon is 1:0.1-0.15; the reaction process is as follows:
。
further, in the step S1, the content of the modified polyvinyl alcohol in the aqueous solution of the modified polyvinyl alcohol and the sodium alginate is 6-10%, and the content of the sodium alginate is 1-2%; the number of thalli in the composite bacterial liquid containing lactobacillus plantarum, bacillus licheniformis and bacillus thuringiensis is more than or equal to 10 9 cfu/mL; the mass ratio of the lactobacillus plantarum to the bacillus licheniformis to the bacillus thuringiensis is 4-6:2-4:1.
Further, the preparation method of the aqueous solution of the modified polyvinyl alcohol and the sodium alginate in the step S1 comprises the following steps: adding modified polyvinyl alcohol into water, heating to 90-95 ℃, stirring for dissolving, then adding sodium alginate, continuously stirring for 1-1.5h, and cooling to room temperature to obtain the modified polyvinyl alcohol.
Further, the preparation method of the composite bacterial liquid containing lactobacillus plantarum, bacillus licheniformis and bacillus thuringiensis in the step S1 comprises the following steps: preparing NA liquid culture medium, inoculating Lactobacillus plantarum, bacillus licheniformis and Bacillus thuringiensis respectively, culturing at 30deg.C for 24-48 hr, counting the number of thallus in the culture solution with a blood cell counting plate under microscope, wherein the number of thallus is greater than or equal to 10 9 And (3) stopping culturing when cfu/mL is carried out, and mixing and dispersing the cfu/mL in sterile water according to the mass ratio to obtain the composite bacterial liquid.
Further, in the step S2, the mass ratio of the gulfweed powder to the 75-85% ethanol solution is 1:20-30; the dosage of the cellulase is 0.8-1.0g/L; the dosage of Tween 80 is 1.4-1.8g/L; the microwave extraction power is 500-600W, and the microwave extraction temperature is 50-60 ℃.
Further, the adding amount of the sodium carboxymethyl cellulose in the step S3 is 2-4g/L; the adding amount of the sodium polyaspartate is 1-3g/L; the addition amount of the organic fertilizer is 20-40g/L; the addition amount of the immobilized microorganism is 20-30g/L; the organic fertilizer is one or more of bone powder, raw hide powder, rapeseed powder, soybean powder and fish meal; the modified polyvinyl alcohol is coated with 8-10% of modified polyvinyl alcohol aqueous solution by mass percent.
The application provides a plant growth promoter for immobilizing microorganisms, which is prepared by the preparation method of the plant growth promoter.
The application has the following beneficial effects:
according to the application, polyvinyl alcohol is used as a raw material, and is subjected to condensation reaction with a compound 1 containing benzene ring and ester groups under the action of N, N' -carbonyl diimidazole to obtain modified polyvinyl alcohol, wherein amide, ester groups and benzene ring groups are introduced into the molecular structure of the modified polyvinyl alcohol, wherein relatively stable conjugated double bonds exist in the benzene ring groups, so that the stability of polymer molecular chains is improved, compared with the polyvinyl alcohol, the added branched chains of the modified polyvinyl alcohol can increase the friction force between the molecular chains, which is equivalent to the increase of intermolecular acting force, and the mechanical property of the modified polyvinyl alcohol is improved; the amide and the ester have chelation to metal ions, and can be used by being compounded with the fertilizer synergist sodium polyaspartate, so that nitrogen, phosphorus, potassium and trace elements in the active ingredients can be synergistically enriched to supply to plants, the plants can more effectively utilize the fertilizer, and the yield and quality of crops are further improved.
The modified polyvinyl alcohol with good mechanical properties is used for coating the carrier for immobilizing microorganisms and the plant growth promoter, and can endow the immobilized microorganisms and the plant growth promoter with certain external impact resistance, so that the plant growth promoter and the immobilized microorganisms contained in the plant growth promoter are prevented from being broken, damaged and deteriorated under the influence of external adverse factors, and the fertilization effect of the plant growth promoter is affected. On one hand, the modified polyvinyl alcohol and sodium alginate are used as carriers, a safer and more stable immobilization environment is provided for microorganisms, the activity of the microorganisms can be maintained, more microorganisms can be continuously released into soil to play a role, and the bioavailability of the microorganisms is improved; on the other hand, the plant growth promoter coated by the modified polyvinyl alcohol has good slow release effect, and can slowly release the effective components such as organic fertilizer, gulfweed extract, microorganism and the like, so that the soil can keep high fertility for a long time, and the plant growth is continuously promoted; wherein the gulfweed extract is rich in polysaccharide substances, cytokinin, purine substances and the like, and can play a role in avoiding partial pests, improve the stress resistance and disease resistance of plants and promote better growth of the plants.
The prepared plant growth promoter is applied to tomatoes, so that the flowers and fruits of the tomatoes can be prevented from falling, the fruit setting rate is increased, the yield is increased, and the yield is increased by more than 35%; meanwhile, the plant growth promoter is also suitable for crops such as cucumber, radish, celery, pumpkin, peanut, rice, wheat, corn and the like, has a wider application range, and can meet market demands better.
Drawings
FIG. 1 is a graph showing the trend of the number of microbial cells in soil at different periods.
Detailed Description
The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
The raw materials used in the following examples are all common commercial products. Wherein, the CAS number of the p-nitrophenethyl alcohol is 100-27-6; triethylamine CAS number 121-44-8; benzoyl chloride CAS number 98-88-4; palladium on carbon CAS No. 7440-05-3; n, N' -carbonyldiimidazole CAS number 530-62-1; n, N-dimethylformamide CAS number 68-12-2; methanol CAS number 67-56-1; dimethyl sulfoxide CAS number 67-68-5; sodium alginate CAS number 9005-38-3; calcium chloride CAS number 10043-52-4; boric acid CAS number 10043-35-3; sodium carboxymethylcellulose CAS number 9004-32-4 (viscosity: 300-800 mpa.s); sodium polyaspartate CAS number 181828-06-8 (M.W7000-8000); polyvinyl alcohol CAS number 9002-89-5 (1799 type); the lactobacillus plantarum is numbered ACCC 11118 and is purchased from China center for type culture collection of agricultural microorganisms; the bacillus licheniformis is numbered ACCC 04369 and is purchased from China center for type culture collection of agricultural microorganisms; bacillus thuringiensis, accession number ACCC 11188, was purchased from China center for type culture collection of agricultural microorganisms.
Example 1
The embodiment provides a preparation method of a plant growth promoter immobilized with microorganisms, which comprises the following steps:
s1, uniformly mixing an aqueous solution of modified polyvinyl alcohol and sodium alginate with a composite bacterial solution containing lactobacillus plantarum, bacillus licheniformis and bacillus thuringiensis according to a mass ratio of 1:1, dropwise adding the mixture into a saturated boric acid solution of 2% calcium chloride by using a syringe, crosslinking and curing for 4 hours, and washing with sterile water for 2 times to obtain immobilized microorganisms; wherein the content of the modified polyvinyl alcohol in the aqueous solution of the modified polyvinyl alcohol and the sodium alginate is 10 percent, and the content of the sodium alginate is 2 percent; the number of thallus in the composite bacterial liquid containing lactobacillus plantarum, bacillus licheniformis and bacillus thuringiensis is more than or equal to 10 9 cfu/mL; lactobacillus plantarum, bacillus licheniformis and Bacillus thuringiensisThe mass ratio is 6:4:1.
S2, crushing gulfweed, sieving with a 70-mesh sieve to obtain gulfweed powder, sequentially adding the gulfweed powder, cellulase and Tween 80 into a 75% ethanol solution, soaking for 30min, placing into a microwave extractor, extracting for 6min by microwaves, filtering, and evaporating ethanol from filtrate under reduced pressure to obtain a gulfweed extract; wherein the mass ratio of the gulfweed powder to the 75% ethanol solution is 1:25; the dosage of the cellulase is 0.8g/L; the dosage of Tween 80 is 1.4g/L; the microwave extraction power is 600W, and the microwave extraction temperature is 60 ℃;
s3, sequentially adding sodium carboxymethyl cellulose, sodium polyaspartate and organic fertilizer into the gulfweed extract, stirring and mixing uniformly, adding immobilized microorganisms, mixing uniformly, granulating, coating by modified polyvinyl alcohol, and drying to obtain a plant growth promoter; wherein the adding amount of the sodium carboxymethyl cellulose is 4g/L; the adding amount of the sodium polyaspartate is 3g/L; the addition amount of the organic fertilizer is 40g/L; the addition amount of the immobilized microorganism is 30g/L; the organic fertilizer is bone meal; the modified polyvinyl alcohol is coated with 10% of modified polyvinyl alcohol aqueous solution by mass fraction.
The preparation method of the aqueous solution of the modified polyvinyl alcohol and the sodium alginate comprises the following steps: adding the modified polyvinyl alcohol into water, heating to 95 ℃, stirring for dissolution, then adding sodium alginate, continuously stirring for 1.5h, and cooling to room temperature to obtain the modified polyvinyl alcohol.
The preparation method of the composite bacterial liquid containing lactobacillus plantarum, bacillus licheniformis and bacillus thuringiensis comprises the following steps: preparing NA liquid culture medium, inoculating Lactobacillus plantarum, bacillus licheniformis and Bacillus thuringiensis respectively, culturing at 30deg.C for 24-48 hr, counting the number of thallus in the culture solution with a blood cell counting plate under microscope, wherein the number of thallus is greater than or equal to 10 9 Terminating the culture when cfu/mL, and mixing and dispersing in sterile water according to the mass ratio to obtain a composite bacterial liquid;
the preparation method of the modified polyvinyl alcohol comprises the following steps: adding polyvinyl alcohol into dimethyl sulfoxide solution, heating to 95 ℃, stirring until the polyvinyl alcohol is dissolved, naturally cooling to room temperature, adding N, N' -carbonyl diimidazole into the reaction solution, continuously reacting for 4 hours, adding a compound 1, heating to 50 ℃, reacting for 10 hours, naturally cooling to room temperature after the reaction is finished, adding 25% ammonia water, stirring for 3 hours, adjusting pH to 5-6 by using dilute hydrochloric acid, filtering, washing filter residues with water for 2 times, and drying to obtain modified polypropylene alcohol; the substitution degree of compound 1 was 21.4% as determined by elemental analysis; the prepared modified polypropylene alcohol has fluorescence absorption under an ultraviolet lamp (254 nm). Wherein the mass ratio of the polyvinyl alcohol to the N, N' -carbonyl diimidazole to the compound 1 is 1:1.1:0.6; the dosage of dimethyl sulfoxide is 25 times of the mass of polyvinyl alcohol, and the dosage of 25% ammonia water is half of the mass of N, N' -carbonyl diimidazole; the reaction process is as follows:
。
the preparation method of the compound 1 comprises the following steps:
(1) Adding 32.0g of p-nitroethanol and 38.7g of triethylamine into 950mL of anhydrous N, N-dimethylformamide solution, slowly dropwise adding 48.4g of benzoyl chloride, heating to 40 ℃ after completion of the dropwise addition, reacting for 2 hours, naturally cooling to room temperature, adding 950mL of water and 1900mL of ethyl acetate for extraction, carrying out water phase back extraction once, collecting an organic phase, and concentrating under reduced pressure to obtain 48.5g of compound 2, wherein the molar ratio of the p-nitroethanol to the benzoyl chloride to the triethylamine is 1:1.8:2.0; the reaction process is as follows:
；
compound 2: ESI (m/z): 272.2[ M+H ]] + ， 1 H-NMR(600MHz，DMSO-d 6 ，δppm)：8.14（d，J=7.8Hz，2H），8.06（d，J=8.6Hz，2H），7.68-7.69（m，1H），7.52-7.56（m，4H），4.60（t，J=7.0Hz，2H），3.14（t，J=7.1Hz，2H）。
(2) Adding 48.5g of compound 2 and 4.85g of palladium-carbon into 900mL of methanol solution, introducing 0.1 MPa of hydrogen, reacting for 6-8 hours at room temperature, filtering the palladium-carbon after the reaction is finished, washing with 50mL of methanol, and concentrating the filtrate under reduced pressure to obtain 36.6g of compound 1, wherein the mass ratio of the compound 2 to the palladium-carbon is 1:0.1; the reaction process is as follows:
。
compound 1: ESI (m/z): 242.2[ M+H ]] + ， 1 H-NMR(600MHz，DMSO-d 6 ，δppm)：8.06（d，J=8.6Hz，2H），7.68-7.69（m，1H），7.55-7.57（m，2H），6.92（d，J=7.8Hz，2H），6.45（d，J=7.8Hz，2H），4.92（s，2H），4.58（t，J=7.0Hz，2H），3.02（t，J=7.1Hz，2H）。
The mechanical property test is carried out on the prepared modified polyvinyl alcohol: the unmodified polyvinyl alcohol was used as a control to prepare aqueous solutions of modified polyvinyl alcohol and polyvinyl alcohol having concentrations of 6%, 8%, 10% and 12%, and then the aqueous solutions were coated to prepare films having a thickness of 80. Mu.m, and the films were cut into strips of 15 mm. Times.120 mm with reference to GB/T1040.3-2006, and the intelligent electronic tensile machine parameters were set according to ASTM D882-09, each sample was tested 6 times, data were recorded, and the results were averaged, as shown in Table 1.
TABLE 1 mechanical Properties of modified Poly (propenol)
As shown in the results of Table 1, at the concentrations of 6%, 8%, 10% and 12%, the mechanical properties (tensile strength and elongation at break) of the modified polyvinyl alcohol are far better than those of the unmodified polyvinyl alcohol, which indicates that the modified polyvinyl alcohol prepared by the method has good mechanical properties, when the concentration is 10%, the tensile strength and elongation at break of the formed modified polyvinyl alcohol film are highest, and the molecular structure of the modified polyvinyl alcohol is analyzed, and amide, ester and benzene ring groups are introduced into part of hydroxyl groups in the molecular structure of the polyvinyl alcohol, wherein relatively stable conjugated double bonds exist in the benzene ring groups, so that the stability of polymer molecular chains is improved, and compared with the polyvinyl alcohol, the added branched chains of the modified polyvinyl alcohol can increase the friction force between the molecular chains, which is equivalent to the increase of the intermolecular acting force, so that the mechanical properties of the modified polyvinyl alcohol are improved; the prepared modified polyvinyl alcohol is applied to the coating of the immobilized microorganism and the plant growth promoter, and the modified polyvinyl alcohol with good mechanical property can endow the immobilized microorganism and the plant growth promoter with a certain external impact resistance, and prevent the plant growth promoter and the immobilized microorganism contained in the plant growth promoter from cracking, damaging and deteriorating under the influence of external adverse factors, thereby influencing the fertilization effect of the plant growth promoter.
Example 2
The embodiment provides a preparation method of a plant growth promoter immobilized with microorganisms, which comprises the following steps:
s1, uniformly mixing an aqueous solution of modified polyvinyl alcohol and sodium alginate with a composite bacterial solution containing lactobacillus plantarum, bacillus licheniformis and bacillus thuringiensis according to a mass ratio of 1:0.8, dropwise adding the mixture into a saturated boric acid solution containing 1% of calcium chloride by using a syringe, crosslinking and curing the mixture for 3 hours, and washing the mixture with sterile water for 2 times to obtain immobilized microorganisms; wherein the content of the modified polyvinyl alcohol in the aqueous solution of the modified polyvinyl alcohol and the sodium alginate is 6 percent, and the content of the sodium alginate is 1 percent; the number of thallus in the composite bacterial liquid containing lactobacillus plantarum, bacillus licheniformis and bacillus thuringiensis is more than or equal to 10 9 cfu/mL; the mass ratio of the lactobacillus plantarum to the bacillus licheniformis to the bacillus thuringiensis is 4:2:1.
S2, the same as the step S2 in the embodiment 1;
s3, the same as the step S3 in the embodiment 1.
The preparation methods of the aqueous solution of the modified polyvinyl alcohol and the sodium alginate, the preparation method of the composite bacterial liquid containing the lactobacillus plantarum, the bacillus licheniformis and the bacillus thuringiensis and the preparation method of the modified polyvinyl alcohol are described in example 1.
Example 3
The embodiment provides a preparation method of a plant growth promoter immobilized with microorganisms, which comprises the following steps:
s1, the same as the step S1 in the embodiment 1;
s2, crushing gulfweed, sieving with a 70-mesh sieve to obtain gulfweed powder, sequentially adding the gulfweed powder, cellulase and Tween 80 into an 85% ethanol solution, soaking for 30min, placing into a microwave extractor, extracting for 6min by microwaves, filtering, and evaporating ethanol from filtrate under reduced pressure to obtain a gulfweed extract; wherein the mass ratio of the gulfweed powder to the 85% ethanol solution is 1:25; the dosage of the cellulase is 1.0g/L; the dosage of Tween 80 is 1.8g/L; the microwave extraction power is 600W, and the microwave extraction temperature is 50 ℃;
s3, sequentially adding sodium carboxymethyl cellulose, sodium polyaspartate and organic fertilizer into the gulfweed extract, stirring and mixing uniformly, adding immobilized microorganisms, mixing uniformly, granulating, coating by modified polyvinyl alcohol, and drying to obtain a plant growth promoter; wherein the adding amount of the sodium carboxymethyl cellulose is 2g/L; the adding amount of the sodium polyaspartate is 1g/L; the addition amount of the organic fertilizer is 20g/L; the addition amount of the immobilized microorganism is 20g/L; the organic fertilizer is fish meal; the modified polyvinyl alcohol is coated with 8% of modified polyvinyl alcohol aqueous solution by mass fraction.
The preparation methods of the aqueous solution of the modified polyvinyl alcohol and the sodium alginate, the preparation method of the composite bacterial liquid containing the lactobacillus plantarum, the bacillus licheniformis and the bacillus thuringiensis and the preparation method of the modified polyvinyl alcohol are described in example 1.
Comparative example 1
The comparative example provides a method for preparing a plant growth promoter immobilized with microorganisms, wherein in step S1, polyvinyl alcohol and an aqueous solution of sodium alginate are used for immobilizing microorganisms, in step S3, the microorganism is coated with polyvinyl alcohol after granulation, and the rest is the same as in example 1.
Comparative example 2
In the present comparative example, a method for preparing a plant growth promoter immobilized with microorganisms was provided, in which in step S1, only an aqueous solution of sodium alginate was used to immobilize microorganisms, in step S3, and no coating was performed after granulation, in comparison with example 1, and the remainder was the same as in example 1.
Test 1 test the yield-increasing and accelerating effects of plant growth promoters on field tomatoes
The test land is set in muu county in Zhengzhou, the test Tian Deshi is flat, the fertility is medium and even, the organic matter content of soil is 12.1g/kg, and the pH=7.2. The tomato seedlings in the growing period of the same crop are divided into 6 areas, wherein the 1 st to 5 th areas (test areas) are respectively fertilized with the plant growth promoters prepared in the examples 1 to 3 and the comparative examples 1 to 2 at one time, no additional fertilization is carried out, the 6 th area (control area) is treated with clear water in the same way, the number of fruits (flowers) in the flowering period, the young fruit period and the harvest time is respectively investigated 30 days after fertilization, and the average fruit setting rate and the yield are calculated; where fruit setting rate = number of fruits harvested/number of flowers x 100%, yield increase = (test area yield-control area yield)/control area yield x 100%; the results are shown in Table 2, where the data are averages of 5 replicates.
TABLE 2 comparison of plant growth promoters to tomato growth effects
As can be seen from the results of Table 2, the plant growth promoters in examples 1-3 all had better growth promoting effect on tomatoes than those in comparative examples 1-2 and the control zone; compared with comparative example 1 (the microorganism-immobilized carrier is polyvinyl alcohol and sodium alginate, and the polyvinyl alcohol is used for coating after pelleting), comparative example 2 (the microorganism-immobilized carrier is sodium alginate and the coating is not performed after pelleting), and a control area (clear water is applied), the plant growth promoter prepared in examples 1-3 (the microorganism-immobilized carrier is modified polyvinyl alcohol and sodium alginate and the modified polyvinyl alcohol is used for coating after pelleting) can prevent tomatoes from flower and fruit dropping, increase fruit setting rate, shorten maturity period, advance marketing and increase yield (more than 35 percent yield); the amide and ester groups in the modified polyvinyl alcohol molecular structure have chelation effect on metal ions, and can be used by being compounded with the fertilizer synergist sodium polyaspartate, so that nitrogen, phosphorus, potassium and trace elements can be synergistically enriched to supply to plants, the plants can more effectively utilize the fertilizer, and the yield and quality of crops are further improved.
At the same time, on days 1, 3, 5, 7, 9 and 11 after fertilization, soil at the same height in the 1 st to 5 th areas (test areas) of the same batch is sampled, microorganisms in the soil samples are detected by a dilution coating method, and the results are shown in figure 1 and correspond toComparative example 1 (polyvinyl alcohol as carrier for immobilizing microorganisms and sodium alginate, coating with polyvinyl alcohol after pelleting), comparative example 2 (sodium alginate as carrier for immobilizing microorganisms, not coating after pelleting), the plant growth promoters prepared in examples 1-3 were able to be released slowly in soil, the number of microbial cells in soil increased slowly with time, and on day 11, the number of microbial cells in soil reached 11×10 5 cuf/g or more; the plant growth promoter prepared by the application has good slow release effect due to the coating of the modified polyvinyl alcohol on the outer layer, the microbial cell quantity in the soil is higher than that of the comparative examples 1-2 after slow release for a period of time, and the use of the modified polyvinyl alcohol and sodium alginate as carriers provides safer and more stable immobilization environment for microorganisms, can maintain the activity of the microorganisms, enables more microorganisms to be released into the soil continuously to play a role, and improves the bioavailability of the microorganisms.
It should be noted that in this document, terms such as "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Although embodiments of the present application have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the application, the scope of which is defined in the appended claims and their equivalents.
Claims (10)
1. A method for preparing a plant growth promoter immobilized with microorganisms, which is characterized by comprising the following steps:
s1, uniformly mixing an aqueous solution of modified polyvinyl alcohol and sodium alginate with a composite bacterial solution containing lactobacillus plantarum, bacillus licheniformis and bacillus thuringiensis according to a mass ratio of 1:0.8-1, dripping the mixture into a saturated boric acid solution containing 1-2% of calcium chloride by using a syringe, crosslinking and curing the mixture for 2-4 hours, and washing the mixture with sterile water for 2 times to obtain immobilized microorganisms;
s2, crushing gulfweed, sieving with a 40-70 mesh sieve to obtain gulfweed powder, sequentially adding the gulfweed powder, cellulase and Tween 80 into a 75-85% ethanol solution, soaking for 20-30min, placing into a microwave extractor, extracting for 4-6min by microwaves, filtering, and evaporating ethanol from filtrate under reduced pressure to obtain a gulfweed extract;
s3, sequentially adding sodium carboxymethyl cellulose, sodium polyaspartate and organic fertilizer into the gulfweed extract, stirring and mixing uniformly, adding immobilized microorganisms, mixing uniformly, granulating, coating by modified polyvinyl alcohol, and drying to obtain a plant growth promoter;
the preparation method of the modified polyvinyl alcohol comprises the following steps: adding polyvinyl alcohol into dimethyl sulfoxide solution, heating to 95 ℃, stirring until the polyvinyl alcohol is dissolved, naturally cooling to room temperature, adding N, N' -carbonyl diimidazole into the reaction solution, continuously reacting for 2-4h, adding compound 1, heating to 50-60 ℃, and reacting for 8-10h to obtain modified polyvinyl alcohol; the chemical structural formula of the compound 1 is as follows:
。
2. the method for preparing a plant growth promoter immobilized with microorganisms according to claim 1, wherein the method for preparing the compound 1 is as follows:
(1) Adding p-nitroethanol and triethylamine into anhydrous N, N-dimethylformamide solution, slowly dropwise adding benzoyl chloride, heating to 40-50 ℃ after dropwise adding, and reacting for 2-3h to obtain a compound 2 with a chemical structural formula:
；
(2) Adding the compound 2 and palladium carbon into a methanol solution, introducing hydrogen with the pressure of 0.1 MPa, and reacting for 6-8 hours at room temperature to obtain the compound 1.
3. The method for preparing a plant growth promoter immobilized with microorganisms according to claim 2, wherein the molar ratio of p-nitroethanol, benzoyl chloride and triethylamine in the step (1) is 1:1.5-1.8:1.5-2.0; and (3) in the step (2), the mass ratio of the compound 2 to the palladium-carbon is 1:0.1-0.15.
4. The method for preparing a plant growth promoter immobilized with microorganisms according to claim 1, wherein the mass ratio of the polyvinyl alcohol to the N, N' -carbonyldiimidazole to the compound 1 in the preparation method of the modified polyvinyl alcohol is 1:1.1-1.2:0.6-0.8.
5. The method for preparing a plant growth promoter immobilized with microorganisms according to claim 1, wherein the content of the modified polyvinyl alcohol in the aqueous solution of the modified polyvinyl alcohol and sodium alginate in the step S1 is 6-10% and the content of the sodium alginate is 1-2%.
6. The method for preparing a microorganism-immobilized plant growth promoter according to claim 1, wherein the number of cells in the composite bacterial liquid containing lactobacillus plantarum, bacillus licheniformis and bacillus thuringiensis in the step S1 is 10 or more 9 cfu/mL; the mass ratio of the lactobacillus plantarum to the bacillus licheniformis to the bacillus thuringiensis is 4-6:2-4:1.
7. The method for preparing a microorganism-immobilized plant growth promoter according to claim 1, wherein the mass ratio of the gulfweed powder to the 75-85% ethanol solution in the step S2 is 1:20-30; the dosage of the cellulase is 0.8-1.0g/L; the dosage of Tween 80 is 1.4-1.8g/L; the microwave extraction power is 500-600W, and the microwave extraction temperature is 50-60 ℃.
8. The method for preparing a plant growth promoter immobilized with microorganisms according to claim 1, wherein the sodium carboxymethyl cellulose is added in an amount of 2-4g/L in step S3; the adding amount of the sodium polyaspartate is 1-3g/L; the addition amount of the organic fertilizer is 20-40g/L; the addition amount of the immobilized microorganism is 20-30g/L.
9. The method for preparing a plant growth promoter immobilized with microorganisms according to claim 1, wherein the organic fertilizer in the step S3 is one or more of bone meal, rawhide, rapeseed meal, soybean meal and fish meal; the modified polyvinyl alcohol is coated with 8-10% of modified polyvinyl alcohol aqueous solution by mass percent.
10. A plant growth promoter immobilized with a microorganism, characterized by being produced by the method for producing a plant growth promoter according to any one of claims 1 to 9.
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