CN116270360A - Honeysuckle flower fermentation liquor and preparation method and application thereof - Google Patents
Honeysuckle flower fermentation liquor and preparation method and application thereof Download PDFInfo
- Publication number
- CN116270360A CN116270360A CN202310398204.4A CN202310398204A CN116270360A CN 116270360 A CN116270360 A CN 116270360A CN 202310398204 A CN202310398204 A CN 202310398204A CN 116270360 A CN116270360 A CN 116270360A
- Authority
- CN
- China
- Prior art keywords
- honeysuckle
- fermentation
- preparation
- fermentation broth
- solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 108
- 230000004151 fermentation Effects 0.000 title claims abstract description 108
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 241000205585 Aquilegia canadensis Species 0.000 title claims abstract 27
- 239000007788 liquid Substances 0.000 claims abstract description 33
- 239000002537 cosmetic Substances 0.000 claims abstract description 12
- 230000002087 whitening effect Effects 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 10
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 17
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 17
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 17
- 230000001954 sterilising effect Effects 0.000 claims description 16
- 241000186018 Bifidobacterium adolescentis Species 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims description 10
- 241000894006 Bacteria Species 0.000 claims description 9
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 9
- 239000012456 homogeneous solution Substances 0.000 claims description 9
- 238000011081 inoculation Methods 0.000 claims description 6
- 238000002156 mixing Methods 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 3
- 241000250967 Branchia Species 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 19
- 230000037303 wrinkles Effects 0.000 abstract description 11
- 102000003425 Tyrosinase Human genes 0.000 abstract description 7
- 108060008724 Tyrosinase Proteins 0.000 abstract description 7
- 230000005764 inhibitory process Effects 0.000 abstract description 7
- 206010020751 Hypersensitivity Diseases 0.000 abstract description 3
- 208000026935 allergic disease Diseases 0.000 abstract description 3
- 230000007815 allergy Effects 0.000 abstract description 3
- 238000009499 grossing Methods 0.000 abstract description 2
- 241001570521 Lonicera periclymenum Species 0.000 description 77
- 239000000843 powder Substances 0.000 description 18
- 239000000047 product Substances 0.000 description 12
- 238000002474 experimental method Methods 0.000 description 9
- 230000000052 comparative effect Effects 0.000 description 8
- 238000001816 cooling Methods 0.000 description 8
- 238000012258 culturing Methods 0.000 description 8
- 239000012467 final product Substances 0.000 description 8
- 238000010438 heat treatment Methods 0.000 description 8
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 7
- 239000004480 active ingredient Substances 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 239000006041 probiotic Substances 0.000 description 6
- 235000018291 probiotics Nutrition 0.000 description 6
- 229910001220 stainless steel Inorganic materials 0.000 description 6
- 239000010935 stainless steel Substances 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 230000000529 probiotic effect Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 241000186660 Lactobacillus Species 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 229940039696 lactobacillus Drugs 0.000 description 4
- 239000008176 lyophilized powder Substances 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000009631 Broth culture Methods 0.000 description 3
- 241000628997 Flos Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241000218588 Lactobacillus rhamnosus Species 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 230000003213 activating effect Effects 0.000 description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 230000001804 emulsifying effect Effects 0.000 description 3
- 239000000284 extract Substances 0.000 description 3
- -1 mask Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 229960002181 saccharomyces boulardii Drugs 0.000 description 3
- QFOHBWFCKVYLES-UHFFFAOYSA-N Butylparaben Chemical compound CCCCOC(=O)C1=CC=C(O)C=C1 QFOHBWFCKVYLES-UHFFFAOYSA-N 0.000 description 2
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 2
- 244000199885 Lactobacillus bulgaricus Species 0.000 description 2
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 description 2
- 241000186605 Lactobacillus paracasei Species 0.000 description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- POJWUDADGALRAB-UHFFFAOYSA-N allantoin Chemical compound NC(=O)NC1NC(=O)NC1=O POJWUDADGALRAB-UHFFFAOYSA-N 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 230000003750 conditioning effect Effects 0.000 description 2
- 239000003995 emulsifying agent Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007717 exclusion Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- BJRNKVDFDLYUGJ-RMPHRYRLSA-N hydroquinone O-beta-D-glucopyranoside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-RMPHRYRLSA-N 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 229940004208 lactobacillus bulgaricus Drugs 0.000 description 2
- LXCFILQKKLGQFO-UHFFFAOYSA-N methylparaben Chemical compound COC(=O)C1=CC=C(O)C=C1 LXCFILQKKLGQFO-UHFFFAOYSA-N 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- QELSKZZBTMNZEB-UHFFFAOYSA-N propylparaben Chemical compound CCCOC(=O)C1=CC=C(O)C=C1 QELSKZZBTMNZEB-UHFFFAOYSA-N 0.000 description 2
- 239000002562 thickening agent Substances 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 description 1
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 description 1
- CHHHXKFHOYLYRE-UHFFFAOYSA-M 2,4-Hexadienoic acid, potassium salt (1:1), (2E,4E)- Chemical compound [K+].CC=CC=CC([O-])=O CHHHXKFHOYLYRE-UHFFFAOYSA-M 0.000 description 1
- HIQIXEFWDLTDED-UHFFFAOYSA-N 4-hydroxy-1-piperidin-4-ylpyrrolidin-2-one Chemical compound O=C1CC(O)CN1C1CCNCC1 HIQIXEFWDLTDED-UHFFFAOYSA-N 0.000 description 1
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 description 1
- POJWUDADGALRAB-PVQJCKRUSA-N Allantoin Natural products NC(=O)N[C@@H]1NC(=O)NC1=O POJWUDADGALRAB-PVQJCKRUSA-N 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 229920002498 Beta-glucan Polymers 0.000 description 1
- YDNKGFDKKRUKPY-JHOUSYSJSA-N C16 ceramide Natural products CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)C=CCCCCCCCCCCCCC YDNKGFDKKRUKPY-JHOUSYSJSA-N 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 241000208828 Caprifoliaceae Species 0.000 description 1
- 241000700198 Cavia Species 0.000 description 1
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
- FBPFZTCFMRRESA-JGWLITMVSA-N D-glucitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-JGWLITMVSA-N 0.000 description 1
- 206010012442 Dermatitis contact Diseases 0.000 description 1
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 description 1
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 description 1
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical group [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- XPJVKCRENWUEJH-UHFFFAOYSA-N Isobutylparaben Chemical compound CC(C)COC(=O)C1=CC=C(O)C=C1 XPJVKCRENWUEJH-UHFFFAOYSA-N 0.000 description 1
- 244000167230 Lonicera japonica Species 0.000 description 1
- 235000017617 Lonicera japonica Nutrition 0.000 description 1
- 239000004909 Moisturizer Substances 0.000 description 1
- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000012641 Pigmentation disease Diseases 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 229920002385 Sodium hyaluronate Polymers 0.000 description 1
- 229920002125 Sokalan® Polymers 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 description 1
- 235000006468 Thea sinensis Nutrition 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 241000192451 [Candida] boleticola Species 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 229960000458 allantoin Drugs 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 229960000271 arbutin Drugs 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- BQMNFPBUAQPINY-UHFFFAOYSA-N azane;2-methyl-2-(prop-2-enoylamino)propane-1-sulfonic acid Chemical compound [NH4+].[O-]S(=O)(=O)CC(C)(C)NC(=O)C=C BQMNFPBUAQPINY-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 210000000133 brain stem Anatomy 0.000 description 1
- 229940067596 butylparaben Drugs 0.000 description 1
- 229960001631 carbomer Drugs 0.000 description 1
- MRUAUOIMASANKQ-UHFFFAOYSA-O carboxymethyl-[3-(dodecanoylamino)propyl]-dimethylazanium Chemical compound CCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC(O)=O MRUAUOIMASANKQ-UHFFFAOYSA-O 0.000 description 1
- 229940106189 ceramide Drugs 0.000 description 1
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 description 1
- 239000007795 chemical reaction product Substances 0.000 description 1
- 208000010247 contact dermatitis Diseases 0.000 description 1
- 229920001577 copolymer Polymers 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- SZXQTJUDPRGNJN-UHFFFAOYSA-N dipropylene glycol Chemical compound OCCCOCCCO SZXQTJUDPRGNJN-UHFFFAOYSA-N 0.000 description 1
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 description 1
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000018927 edible plant Nutrition 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 229960001617 ethyl hydroxybenzoate Drugs 0.000 description 1
- 235000010228 ethyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004403 ethyl p-hydroxybenzoate Substances 0.000 description 1
- NUVBSKCKDOMJSU-UHFFFAOYSA-N ethylparaben Chemical compound CCOC(=O)C1=CC=C(O)C=C1 NUVBSKCKDOMJSU-UHFFFAOYSA-N 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 239000006260 foam Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000007760 free radical scavenging Effects 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 229940074928 isopropyl myristate Drugs 0.000 description 1
- BEJNERDRQOWKJM-UHFFFAOYSA-N kojic acid Chemical compound OCC1=CC(=O)C(O)=CO1 BEJNERDRQOWKJM-UHFFFAOYSA-N 0.000 description 1
- 229960004705 kojic acid Drugs 0.000 description 1
- WZNJWVWKTVETCG-UHFFFAOYSA-N kojic acid Natural products OC(=O)C(N)CN1C=CC(=O)C(O)=C1 WZNJWVWKTVETCG-UHFFFAOYSA-N 0.000 description 1
- 230000006651 lactation Effects 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 229940075468 lauramidopropyl betaine Drugs 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000006210 lotion Substances 0.000 description 1
- 230000008099 melanin synthesis Effects 0.000 description 1
- 230000003061 melanogenesis Effects 0.000 description 1
- 201000001441 melanoma Diseases 0.000 description 1
- 235000010270 methyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004292 methyl p-hydroxybenzoate Substances 0.000 description 1
- 229960002216 methylparaben Drugs 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001333 moisturizer Effects 0.000 description 1
- 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
- 235000019799 monosodium phosphate Nutrition 0.000 description 1
- LWGJTAZLEJHCPA-UHFFFAOYSA-N n-(2-chloroethyl)-n-nitrosomorpholine-4-carboxamide Chemical compound ClCCN(N=O)C(=O)N1CCOCC1 LWGJTAZLEJHCPA-UHFFFAOYSA-N 0.000 description 1
- RGUVUPQQFXCJFC-UHFFFAOYSA-N n-hydroxyoctanamide Chemical group CCCCCCCC(=O)NO RGUVUPQQFXCJFC-UHFFFAOYSA-N 0.000 description 1
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 235000020333 oolong tea Nutrition 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- BJRNKVDFDLYUGJ-UHFFFAOYSA-N p-hydroxyphenyl beta-D-alloside Natural products OC1C(O)C(O)C(CO)OC1OC1=CC=C(O)C=C1 BJRNKVDFDLYUGJ-UHFFFAOYSA-N 0.000 description 1
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 235000010241 potassium sorbate Nutrition 0.000 description 1
- 239000004302 potassium sorbate Substances 0.000 description 1
- 229940069338 potassium sorbate Drugs 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000000899 pressurised-fluid extraction Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 235000010232 propyl p-hydroxybenzoate Nutrition 0.000 description 1
- 239000004405 propyl p-hydroxybenzoate Substances 0.000 description 1
- 229960003415 propylparaben Drugs 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 230000037380 skin damage Effects 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 229960003885 sodium benzoate Drugs 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 229940010747 sodium hyaluronate Drugs 0.000 description 1
- 229940045944 sodium lauroyl glutamate Drugs 0.000 description 1
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 description 1
- IWIUXJGIDSGWDN-UQKRIMTDSA-M sodium;(2s)-2-(dodecanoylamino)pentanedioate;hydron Chemical group [Na+].CCCCCCCCCCCC(=O)N[C@H](C([O-])=O)CCC(O)=O IWIUXJGIDSGWDN-UQKRIMTDSA-M 0.000 description 1
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical group [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 239000011975 tartaric acid Substances 0.000 description 1
- 235000002906 tartaric acid Nutrition 0.000 description 1
- GYDJEQRTZSCIOI-LJGSYFOKSA-N tranexamic acid Chemical compound NC[C@H]1CC[C@H](C(O)=O)CC1 GYDJEQRTZSCIOI-LJGSYFOKSA-N 0.000 description 1
- 229960000401 tranexamic acid Drugs 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
- 239000000230 xanthan gum Substances 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
- 229940082509 xanthan gum Drugs 0.000 description 1
- 235000010493 xanthan gum Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P39/00—Processes involving microorganisms of different genera in the same process, simultaneously
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/85—Products or compounds obtained by fermentation, e.g. yoghurt, beer, wine
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/225—Lactobacillus
- C12R2001/25—Lactobacillus plantarum
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Veterinary Medicine (AREA)
- Mycology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Dermatology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Botany (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Cosmetics (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides honeysuckle flower fermentation liquor and a preparation method and application thereof, and belongs to the technical field of biological fermentation. The invention ferments the honeysuckle homogeneous liquid by means of specific parameters and the secondary fermentation process of specific strains, and solves the problem of limited efficacy of the honeysuckle fermentation product in the prior art. The preparation method disclosed by the invention can be used for remarkably improving the activity of the honeysuckle in the aspect of cosmetic application, and especially improving the whitening and wrinkle removing performances of the honeysuckle. The tyrosinase inhibition rate of the honeysuckle flower fermentation liquor prepared by the method is up to 69.39%, the effects of removing wrinkles and smoothing skin are also obviously improved, and the honeysuckle flower fermentation liquor has the effects of relieving and resisting allergy of the honeysuckle flower and has good application prospects.
Description
Technical Field
The invention relates to the technical field of biological fermentation, in particular to honeysuckle flower fermentation liquor and a preparation method and application thereof.
Background
Honeysuckle flower, also called honeysuckle flower, is a flower of honeysuckle (Lonicera japonica Thunb.) belonging to the family Caprifoliaceae, which is initially white and then yellow, so that the honeysuckle flower is an important medicinal and edible plant, and has the effects of clearing heat, detoxicating, resisting bacteria and viruses, resisting allergy, reducing blood sugar and lipid, resisting oxidization, resisting tumor and the like. The honeysuckle flower contains organic acid, flavone, polysaccharide and other components, has the functions of resisting aging, inhibiting bacteria, diminishing inflammation and the like, and has wide application prospect in the field of cosmetics.
The microbial fermentation extraction method is a process for extracting the plant active ingredients and performing bioconversion, and has the advantages of high yield of the active ingredients, various effects, avoidance of damage to the plant active ingredients, high bioavailability of the active ingredients and the like. The mixed liquor rich in active substances, which is produced by fermenting plant materials by microorganisms, is called plant fermentation extract (Plant Fermentation Extracts, PFEs), has the characteristics of antioxidation, antibiosis, whitening, anti-aging and the like, and is widely applied to the fields of cosmetics and health care products.
CN114668699a discloses a preparation method of honeysuckle fermentation primary pulp, which adopts bacillus subtilis to ferment honeysuckle, and the prepared honeysuckle fermentation primary pulp has active ingredients for removing DPPH free radicals and active ingredients for inhibiting tyrosinase activity and melanin synthesis. CN114948819a discloses a preparation method of a honeysuckle flower ferment with repairing effect, which comprises the steps of adding saccharomycetes and/or lactobacillus for fermentation after the honeysuckle flower is subjected to enzymolysis and sterilization, has free radical scavenging capability, has repairing effect, and can be used for repairing skin damage resistance. However, the improvement degree of the two fermentation technologies on the effect of the honeysuckle is limited, and other existing technologies for fermenting the honeysuckle by utilizing a microbial fermentation extraction method are also required to be improved.
Disclosure of Invention
The invention aims to provide honeysuckle flower fermentation liquor, a preparation method and application thereof, which are used for solving the problem of limited efficacy of honeysuckle flower fermentation products in the prior art.
In order to achieve the above object, the present invention provides the following technical solutions:
the invention provides a preparation method of honeysuckle flower fermentation liquor, which comprises the following steps:
mixing honeysuckle and water, homogenizing to obtain a honeysuckle homogeneous solution;
mixing the honeysuckle homogeneous solution, the Bradyyeast solution and the lactobacillus plantarum solution, and performing primary fermentation to obtain a honeysuckle primary fermentation solution;
sterilizing the primary fermentation liquid of the honeysuckle, inoculating bifidobacterium adolescentis, and performing secondary fermentation to obtain the fermentation liquid of the honeysuckle.
Preferably, the weight ratio of the honeysuckle to the water is 1:0.8-1.2;
the homogenizing rotating speed is 4000-6000 rpm;
the homogenizing time is 5-15 min.
Preferably, the live bacteria concentration of the Saccharomyces boulardii liquid is 1.0X10 9 ～1.0×10 10 CFU/mL；
The viable bacteria concentration of the lactobacillus plantarum bacterial liquid is 1.0x10 9 ～1.0×10 10 CFU/mL。
Preferably, the volume ratio of the honeysuckle homogeneous liquid to the Bradyyeast liquid to the lactobacillus plantarum liquid is 200:3-5:1.
Preferably, the temperature of the first fermentation is 27.5-28.5 ℃;
the rotating speed of the first fermentation is 400-600 rpm;
the time of the first fermentation is 5-10 d.
Preferably, the sterilization adopts normal pressure intermittent sterilization, the normal pressure intermittent sterilization comprises alternately performed working phases and intermittent phases, the temperature of the working phases is 70-80 ℃, the time of the working phases is 50-70 min, the temperature of the intermittent phases is 35-37 ℃, and the time of the intermittent phases is 0.5-1.5 d.
Preferably, the inoculation amount of the bifidobacterium adolescentis is 40-60 g/L.
Preferably, the temperature of the second fermentation is 37-39 ℃;
the rotating speed of the second fermentation is 500-700 rpm;
the time of the second fermentation is 25-35 d.
The invention also provides the honeysuckle flower fermentation liquor obtained by the preparation method.
The invention also provides application of the honeysuckle flower fermentation liquor in preparing cosmetics, wherein the cosmetics are products for relieving, whitening and/or removing wrinkles.
The invention has the technical effects and advantages that:
according to the invention, a group of special strains are obtained through a large number of screening experiments, and the honeysuckle homogeneous liquid is fermented by combining secondary fermentation with specific parameters, so that the activity of the honeysuckle in the aspect of cosmetic application can be remarkably improved, and particularly the whitening and wrinkle removing activities of the honeysuckle can be improved. The tyrosinase inhibition rate of the honeysuckle flower fermentation liquor prepared by the method is up to 69.39%, the effects of removing wrinkles and smoothing skin are also remarkably improved, and the honeysuckle flower fermentation liquor also has the effects of relieving and resisting allergy of the honeysuckle flower and has good application prospects.
Detailed Description
The invention provides a preparation method of honeysuckle flower fermentation liquor, which comprises the following steps: mixing honeysuckle and water, homogenizing to obtain a honeysuckle homogeneous solution; mixing the honeysuckle homogeneous solution, the Bradyyeast solution and the lactobacillus plantarum solution, and performing primary fermentation to obtain a honeysuckle primary fermentation solution; sterilizing the primary fermentation liquid of the honeysuckle, inoculating bifidobacterium adolescentis, and performing secondary fermentation to obtain the fermentation liquid of the honeysuckle; in the invention, the honeysuckle is preferably mixed with water after the impurities are removed by cleaning, and the weight ratio of the honeysuckle to the water is preferably 1:0.8-1.2, and more preferably 1:0.9-1.1; the rotation speed of the homogenization is preferably 4000 to 6000rpm, more preferably 4500 to 5500rpm; by a means ofThe homogenizing time is preferably 5 to 15 minutes, more preferably 8 to 12 minutes; the live bacteria concentration of the Saccharomyces boulardii liquid is preferably 1.0X10 9 ～1.0×10 10 CFU/mL, more preferably 8.0X10 9 ～2.0×10 10 CFU/mL; the viable bacteria concentration of the lactobacillus plantarum bacterial liquid is preferably 1.0x10 9 ～1.0×10 10 CFU/mL, more preferably 7.0X10 9 ～3.0×10 10 CFU/mL. The volume ratio of the honeysuckle homogeneous solution, the Bradyyeast solution and the lactobacillus plantarum solution is preferably 200:3-5:1, and more preferably 200:3.5-4.5:1.
In the present invention, the temperature of the first fermentation is preferably 27.5 to 28.5 ℃, and more preferably 27.8 to 28.2; the rotation speed of the first fermentation is preferably 400-600 rpm, more preferably 450-550 rpm; the time of the first fermentation is preferably 5-10 d, more preferably 7-9 d, the sterilization is preferably an atmospheric intermittent sterilization, the atmospheric intermittent sterilization comprises alternating working phases and intermittent phases, the temperature of the working phases is preferably 70-80 ℃, more preferably 73-77 ℃, the time of the working phases is preferably 50-70 min, more preferably 55-65 min, the temperature of the intermittent phases is preferably 35-37 ℃, more preferably 35.5-36.5 ℃, the time of the intermittent phases is preferably 0.5-1.5 d, more preferably 0.8-1.2 d; the inoculation amount of the bifidobacterium adolescentis is preferably 40-60 g/L, more preferably 45-55 g/L, and the temperature of the second fermentation is preferably 37-39 ℃, more preferably 37.5-38.5 ℃; the rotation speed of the second fermentation is preferably 500-700 rpm, more preferably 550-650 rpm; the time for the second fermentation is preferably 25 to 35d, more preferably 28 to 32d.
The invention also provides the honeysuckle flower fermentation liquor obtained by the preparation method, the fermentation liquor is preferably light yellow in state, the smell is preferably light grass and wood fragrance, no peculiar smell is generated, and meanwhile, the honeysuckle flower fermentation liquor is taken as a sign of successful fermentation.
The invention also provides application of the honeysuckle fermentation liquor in preparing cosmetics, the honeysuckle fermentation liquor is preferably used for preparing whitening and/or wrinkle removing products, the formulation of the whitening and/or wrinkle removing products is preferably cream, mask, emulsion, toning lotion, bath foam, facial cleanser and the like, the addition amount of the honeysuckle fermentation liquor in the products is preferably 5-100%, and more preferably 40-90%, and in the invention, the whitening and/or wrinkle removing products also comprise auxiliary materials acceptable by cosmetics, and the auxiliary materials comprise moisturizers, emulsifiers, thickeners, skin conditioning agents, pH regulators, preservatives, water and the like; the emulsifier is preferably sodium lauroyl glutamate, lauramidopropyl betaine, isopropyl myristate or cocoyl glucoside; the humectant is preferably sodium hyaluronate, glycerol, propylene glycol, butylene glycol, dipropylene glycol, sorbitol, inositol, beta-glucan or trehalose; the thickener is preferably disodium EDTA, carbomer, xanthan gum, ammonium acryloyldimethyl taurate/VP copolymer or sodium polyacrylate grafted starch; the skin conditioning agent is preferably octanoyl hydroxamic acid, allantoin, ceramide, nicotinamide, vitamin C derivatives, arbutin, tranexamic acid, yeast/hydrolytic yeast; the pH regulator is preferably arginine, sodium citrate, citric acid, phosphoric acid, tartaric acid, sodium dihydrogen phosphate and triethanolamine, the preservative is preferably methylparaben, butylparaben, ethylparaben, isobutyl paraben, propyl paraben, potassium sorbate and sodium benzoate, and the auxiliary materials preferably do not influence the efficacy of the honeysuckle fermentation broth.
The technical effects provided by the present invention will be described in detail with reference to examples, but they should not be construed as limiting the scope of the present invention.
Lactobacillus plantarum lyophilized powder (lactobacillus plantarum) SHBCCD 81363, saccharomyces boulardii lyophilized powder (Saccharomyces cerevisiae var boulardii) CNCM I-1079, candida mycota (Candida boleticola) SHBCC D53761, lactobacillus rhamnosus lyophilized powder (Lactobacillus rhamnosus) SHBCC D80286, purchased from Shanghai preservation biotechnology center;
bifidobacterium adolescentis probiotic freeze-dried powder (product number: ZKJY-Bifidobacterium adolescentis) is purchased from the micro-ecological research institute of Carnis nutritional medicine (Shandong) of the middle-jiao, and the viable count is more than or equal to 1.0X10 10 CFU/g；
Lactobacillus paracasei freeze-dried lactobacillus powder (product number: LP 075), and viable count is not less than 1.0X10 10 CFU/g, lactobacillus bulgaricus freeze-dried lactobacillus powder (product number: LB 005), viable count is not less than 1.0X10 10 CFU/g, available from Zhengzhou Bai Bao Biotechnology Co.
Example 1
Putting 1kg of cleaned and impurity-removed honeysuckle flower into a container, adding 1kg of water, homogenizing for 10min by using a homogenizer (SL-RH-LAB 200 homogenizing emulsifying machine) at a rotation speed of 5000rpm to obtain a honeysuckle flower homogenized liquid for later use.
Activating the freeze-dried powder of the Buddy yeast according to the commodity instruction, then passaging twice, and performing expanded culture to 5.0X10 by adopting malt extract broth culture medium without adding agar 9 CFU/mL, lactobacillus plantarum freeze-dried powder is activated according to a commodity instruction and then passaged twice, and the agar-free MRS culture medium is subjected to expansion culture until the concentration is 5.0x10 9 CFU/mL, inoculating 20mL of Bradyyeast liquid and 5mL of lactobacillus plantarum liquid into 1L of honeysuckle homogeneous liquid, transferring a fermentation system into a stainless steel fermentation tank, and fermenting for 7d at 28 ℃ and 500rpm to obtain a honeysuckle primary fermentation liquid.
Intermittently sterilizing the primary fermentation broth of flos Lonicerae, heating at 75deg.C for 60min, cooling to 37deg.C, culturing for 1d, heating at 75deg.C for 60min, and cooling to 37deg.C, and culturing for 12 h.
Inoculating the bifidobacterium adolescentis probiotic freeze-dried powder into the sterilized honeysuckle primary fermentation broth with the inoculation amount of 50g/L, transferring the honeysuckle primary fermentation broth into a stainless steel fermentation tank, and fermenting for 30d at 38 ℃ and 600rpm to obtain a honeysuckle fermentation broth final product.
Example 2
1kg of cleaned and impurity-removed honeysuckle flower is put into a container, 0.8kg of water is added, and the mixture is homogenized for 5min under the condition of 4000rpm of a homogenizer (SL-RH-LAB 200 homogenizing emulsifying machine) to obtain a honeysuckle flower homogenized liquid for standby.
Activating the freeze-dried powder of the Buddy yeast according to the commodity instruction, then passaging twice, and performing expanded culture to 1.0X10 by adopting malt extract broth culture medium without adding agar 9 CFU/mL, plantsThe lactobacillus freeze-dried powder is activated according to the commodity instruction and then passaged twice, and the MRS culture medium without agar is subjected to expansion culture until the concentration is 1.0x10 10 CFU/mL, inoculating 25mL of Bradyyeast liquid and 5mL of lactobacillus plantarum liquid into 1L of honeysuckle homogeneous liquid, transferring a fermentation system into a stainless steel fermentation tank, and fermenting for 5d at 28.5 ℃ and 400rpm to obtain a honeysuckle primary fermentation liquid.
Intermittently sterilizing the primary fermentation broth of flos Lonicerae, heating at 70deg.C for 50min, cooling to 36deg.C, culturing for 1.5d, heating at 75deg.C for 70min again, cooling to 37deg.C, culturing for 0.5d, heating at 75deg.C for 50min again, and cooling to 36deg.C, and culturing for 10 hr.
Inoculating the bifidobacterium adolescentis probiotic freeze-dried powder into the sterilized honeysuckle primary fermentation broth with the inoculation amount of 40g/L, transferring the honeysuckle primary fermentation broth into a stainless steel fermentation tank, and fermenting for 25d at 39 ℃ and 500rpm to obtain a honeysuckle fermentation broth final product.
Example 3
1kg of cleaned and impurity-removed honeysuckle flower is put into a container, 1.2kg of water is added, and the mixture is homogenized for 15min by a homogenizer (SL-RH-LAB 200 homogenizing emulsifying machine) at a rotating speed of 6000rpm, so as to obtain a honeysuckle flower homogenized liquid for standby.
Activating the freeze-dried powder of the Buddy yeast according to the commodity instruction, then passaging twice, and performing expanded culture to 1.0X10 by adopting malt extract broth culture medium without adding agar 10 CFU/mL, lactobacillus plantarum freeze-dried powder is activated according to a commodity instruction and then passaged twice, and the agar-free MRS culture medium is subjected to expansion culture until the concentration is 1.0x10 9 CFU/mL, inoculating 15mL of Bradyyeast liquid and 5mL of lactobacillus plantarum liquid into 1L of honeysuckle homogeneous liquid, transferring a fermentation system into a stainless steel fermentation tank, and fermenting for 10d at the temperature of 27.5 ℃ and the speed of 600rpm to obtain a honeysuckle primary fermentation liquid.
Intermittently sterilizing the primary fermentation broth of flos Lonicerae, heating at 80deg.C for 70min, cooling to 35deg.C, culturing for 0.5d, heating again at 75deg.C for 70min, cooling to 35deg.C, culturing again for 1.5d, heating again at 75deg.C for 50min, and cooling to 35deg.C, and culturing for 14 h.
Inoculating the bifidobacterium adolescentis probiotic freeze-dried powder into the sterilized honeysuckle primary fermentation broth with the inoculation amount of 60g/L, transferring the honeysuckle primary fermentation broth into a stainless steel fermentation tank, and fermenting for 35d at 37 ℃ and 700rpm to obtain a honeysuckle fermentation broth final product.
Comparative example 1
The only difference compared to example 1 is that the brainstem yeast lyophilized powder was replaced with candida mycota.
Comparative example 2
The only difference compared to example 1 is that the lactobacillus plantarum freeze-dried powder is replaced by lactobacillus rhamnosus freeze-dried powder.
Comparative example 3
The only difference compared to example 1 is that the bifidobacterium adolescentis probiotic freeze-dried powder is replaced by lactobacillus paracasei freeze-dried lactic acid bacteria powder.
Comparative example 4
The only difference compared to example 1 is that the freeze-dried powder of bifidobacterium adolescentis probiotics is replaced by the freeze-dried powder of lactobacillus bulgaricus.
Experimental example 1 verification of whitening Effect
The sample sources are shown in table 1 below:
TABLE 1 Experimental sample Source
| Experimental grouping | Sample source |
| Experimental group A | Honeysuckle homogeneous solution in example 1 |
| Experiment group B | Honeysuckle primary fermentation liquor in example 1 |
| Experiment group C | The honeysuckle flower fermentation broth end product in example 1 |
| Experiment group D | The final product of comparative example 1 |
| Experiment group E | The final product of comparative example 2 |
| Experiment group F | The final product of comparative example 3 |
| Experiment group G | The final product of comparative example 4 |
The whitening efficacy of each group of samples was measured separately.
Referring to the method of Aoki Y et al (Aoki Y, tanigawa T, abe H, et al, melanogenesis inhibi-tion by an oolong tea extract in B mouse melanoma cells and UV-induced skin pigmentation in brownish guinea pigs [ J ]. Biosci Biotechnol Biochem,2007,71 (8): 1879-1885.), each group of samples was characterized by tyrosinase inhibition, and the positive control was assayed in triplicate using a 50 μg/mL concentration of kojic acid, and the tyrosinase inhibition (%) was recorded for each group, as shown in Table 2 below:
TABLE 2 tyrosinase inhibition results
The results show that the final product of the honeysuckle fermentation liquor in the embodiment 1 shows the highest tyrosinase inhibition rate, which shows that the whitening effect is optimal, compared with the honeysuckle raw material, the effect is obviously improved, and the effect is superior to other strain combinations.
Experimental example 2
The sample sources were the same as in experimental example 1, and the samples were pasteurized for 30min and then used for the test.
Selecting 35 female volunteers with eye marks, wherein the exclusion conditions meet the exclusion standard of cosmetic contact dermatitis diagnostic standard and treatment principle, the conditions of non-allergic physique or skin diseases exist, and the women in non-pregnant women or lactation period do not participate in other cosmetic experiments 2 months before the experiment; the same experimental group samples were used randomly for every 5 volunteers.
Wrinkle removal performance of each group of samples was tested using a VisioScan VC20 plus skin surface texture tester, and R3 and S were recorded esm The lower these two indices, the smoother the skin, the lower the visibility of wrinkles; the subjects respectively use the same cleaning product to clean the face in the same environment (the temperature is 27 ℃ and the test is started at four afternoon), after sitting still for 30min, the initial values are measured, then the samples are respectively smeared at the eye marks, the samples are massaged to be absorbed, and after 30min, the test is performed again; the test was performed at four afternoon points 7d, 14d, 28d, respectively, using the same procedure each day, and the results were recorded as shown in table 3 below:
table 3 texture test results
The results show that the two-step fermentation method provided by the invention is matched with specific strains, so that the wrinkle removing effect of the honeysuckle fermentation liquid can be improved, the skin is smoother, and the honeysuckle fermentation product provided by the invention can be improved in the effect of delaying aging and has a good application prospect.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (10)
1. The preparation method of the honeysuckle flower fermentation liquor is characterized by comprising the following steps of:
mixing honeysuckle and water, homogenizing to obtain a honeysuckle homogeneous solution;
mixing the honeysuckle homogeneous solution, the Bradyyeast solution and the lactobacillus plantarum solution, and performing primary fermentation to obtain a honeysuckle primary fermentation solution;
sterilizing the primary fermentation liquid of the honeysuckle, inoculating bifidobacterium adolescentis, and performing secondary fermentation to obtain the fermentation liquid of the honeysuckle.
2. The preparation method of the honeysuckle fermentation broth according to claim 1, wherein the weight ratio of the honeysuckle to the water is 1:0.8-1.2;
the homogenizing rotating speed is 4000-6000 rpm;
the homogenizing time is 5-15 min.
3. The method for preparing the honeysuckle fermentation broth according to claim 2, wherein the live bacteria concentration of the brazil yeast liquid is 1.0 x 10 9 ～1.0×10 10 CFU/mL；
The viable bacteria concentration of the lactobacillus plantarum bacterial liquid is 1.0x10 9 ～1.0×10 10 CFU/mL。
4. The preparation method of the honeysuckle fermentation broth according to claim 3, wherein the volume ratio of the honeysuckle homogeneous solution, the branchia yeast solution and the lactobacillus plantarum solution is 200:3-5:1.
5. The method for preparing honeysuckle fermentation broth according to claim 4, wherein the temperature of the first fermentation is 27.5-28.5 ℃;
the rotating speed of the first fermentation is 400-600 rpm;
the time of the first fermentation is 5-10 d.
6. The preparation method of honeysuckle flower fermentation broth according to claim 5, wherein the sterilization adopts normal pressure intermittent sterilization, the normal pressure intermittent sterilization comprises alternately performed working phases and intermittent phases, the temperature of the working phases is 70-80 ℃, the time of the working phases is 50-70 min, the temperature of the intermittent phases is 35-37 ℃, and the time of the intermittent phases is 0.5-1.5 d.
7. The method for preparing a honeysuckle fermentation broth according to claim 6, wherein the inoculation amount of bifidobacterium adolescentis is 40-60 g/L.
8. The method for preparing honeysuckle fermentation broth according to claim 7, wherein the temperature of the second fermentation is 37-39 ℃;
the rotating speed of the second fermentation is 500-700 rpm;
the time of the second fermentation is 25-35 d.
9. The honeysuckle flower fermentation broth obtained by the preparation method of any one of claims 1 to 8.
10. Use of the honeysuckle fermentation broth according to claim 9 for the preparation of a cosmetic product, characterized in that the cosmetic product is a soothing, whitening and/or wrinkle-removing product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310398204.4A CN116270360A (en) | 2023-04-14 | 2023-04-14 | Honeysuckle flower fermentation liquor and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310398204.4A CN116270360A (en) | 2023-04-14 | 2023-04-14 | Honeysuckle flower fermentation liquor and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116270360A true CN116270360A (en) | 2023-06-23 |
Family
ID=86838011
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310398204.4A Pending CN116270360A (en) | 2023-04-14 | 2023-04-14 | Honeysuckle flower fermentation liquor and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116270360A (en) |
-
2023
- 2023-04-14 CN CN202310398204.4A patent/CN116270360A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TWI668009B (en) | Skin external agent for skin whitening comprising an extract of fermented wheat germ | |
| CN106466291B (en) | A kind of still cleaning products and preparation method thereof containing fermented grain object | |
| CN109939064B (en) | Essence containing probiotic fermentation metabolite and application of essence in cosmetics | |
| CN110946792A (en) | Quinoa fermented product and application thereof | |
| CN111789790B (en) | Whitening emulsion containing rose fermentation liquor and preparation method thereof | |
| KR20160049812A (en) | Cosmetic composition for promoting ceramide biosynthesis containing Panax ginseng, honey, and propolis fermented by kimchi lactic acid bacteria | |
| KR101325092B1 (en) | Cosmetic Composition for the Skin Desquamation Comprising the Citrus junos Extracts | |
| CN108042456B (en) | Preparation method and application of dendrobium officinale fermentation product | |
| CN113604395A (en) | Lactobacillus plantarum capable of fermenting dendrobium and improving skin quality by fermentation liquor of dendrobium | |
| CN115038425A (en) | Cosmetic composition for improving skin comprising polysaccharides, yeast extract and fermented product of strain having probiotic properties as effective components | |
| CN114632055B (en) | Whitening and relieving plant fermentation product and preparation method and application thereof | |
| CN111904908B (en) | Essence containing rose fermentation liquor and preparation method thereof | |
| CN111789778B (en) | Face cream containing rose fermentation liquor and preparation method thereof | |
| CN113842341A (en) | Roselle fermentation product and preparation method and application thereof | |
| CN111870550A (en) | Allergy-relieving sunscreen cream containing rose fermentation liquor and preparation method thereof | |
| CN114869840B (en) | Prebiotic face cream and preparation method thereof | |
| CN111870552A (en) | Allergy-relieving mask containing rose fermentation liquor and preparation method thereof | |
| CN104644538B (en) | Radix scutellariae lactobacillus fermentation, the cosmetics containing fermentate and preparation method and purposes | |
| CN106806299A (en) | A kind of thin face frost of green tea marine alga ferment with the fat function that disappears and preparation method thereof | |
| CN116270360A (en) | Honeysuckle flower fermentation liquor and preparation method and application thereof | |
| KR101880470B1 (en) | Cosmetic composition comprising organic acid fermentation and natural polymer for moisturizing effect on the skin | |
| CN111789801B (en) | Moisturizing shower gel containing rose fermentation liquor and preparation method thereof | |
| CN111956546B (en) | Anti-dandruff shampoo containing rose fermentation liquor and preparation method thereof | |
| CN115282093A (en) | Anti-aging wrinkle-removing composition and repair eye cream containing same | |
| CN111888293B (en) | Moisturizing toner containing rose fermentation liquor and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |