CN114869840B

CN114869840B - Prebiotic face cream and preparation method thereof

Info

Publication number: CN114869840B
Application number: CN202210640211.6A
Authority: CN
Inventors: 周威; 毛建林
Original assignee: Campari Science & Technology Suzhou Co ltd
Current assignee: Campari Science & Technology Suzhou Co ltd
Priority date: 2022-06-08
Filing date: 2022-06-08
Publication date: 2023-04-28
Anticipated expiration: 2042-06-08
Also published as: CN114869840A

Abstract

The application relates to the technical field of daily cosmetics, in particular to prebiotic face cream and a preparation method thereof. The prebiotic face cream consists of a prebiotic composition and a matrix formula; the prebiotic composition comprises the following components in parts by weight: 50-60 parts of functional oligosaccharide, 8-12 parts of probiotic components, 8-12 parts of plant extracts, 3-5 parts of emulsification aids, 20-30 parts of deionized water and 15-20 parts of vegetable oil; the prebiotic composition is prepared by ultrasonic mixing. The improvement of the skin surface microecology is realized through the compounding of the prebiotic-functional oligosaccharide, the implanted probiotic-probiotic component and the plant extract, and compared with the single use of the prebiotic component, the probiotic-functional oligosaccharide is favorable for replacing harmful bacteria by the auxiliary probiotic, so that the auxiliary probiotic occupies the dominant position of the skin surface microecology.

Description

Prebiotic face cream and preparation method thereof

Technical Field

The application relates to the technical field of daily cosmetics, in particular to prebiotic face cream and a preparation method thereof.

Background

The cream is a cream-like texture skin care product special for facial skin, can effectively improve various problems of the skin, nourish and protect the skin deeply, and in recent years, along with the pursuit of higher effect of the cream by people, the prebiotic cream capable of improving microecology of the skin surface is generated.

The prebiotics in the related art mainly comprise beta-glucan, fructo-oligosaccharide, xylo-oligosaccharide, galacto-oligosaccharide, isomaltooligosaccharide and other functional oligosaccharides and matrix formulas, and can provide growing food for beneficial bacteria on the skin through the functional oligosaccharides after being coated on the face, so that the growth and breeding of the beneficial bacteria are promoted while moisturizing, repairing and nourishing the skin.

However, the repairing and nourishing effects of the prebiotic face cream are generally limited by the original colony condition of the skin and the interference of external adverse factors, and when the skin environment is changed due to excessive cleaning, antibiotics, ultraviolet rays, lack of nutrition, coldness and other factors, the number of probiotics can be obviously reduced;

and because the prebiotics do not have specificity, when the probiotics and the harmful bacteria are seriously unbalanced, and the harmful bacteria take the dominant place and invade the microecology on the skin surface, the prebiotics can be absorbed by the harmful bacteria, and further propagation of the harmful bacteria is promoted, so that vicious circulation is generated.

Disclosure of Invention

In order to solve the problems, and reduce the risk of further propagation of harmful bacteria and vicious circle caused by unbalanced flora and ingestion of the prebiotics by the harmful bacteria, the application particularly provides the prebiotic cream and a preparation method thereof.

In a first aspect, the present application provides a prebiotic cream, which adopts the following technical scheme:

a prebiotic facial cream comprises prebiotic composition and matrix formula;

the prebiotic composition comprises the following components in parts by weight:

50-60 parts of functional oligosaccharide, 8-12 parts of probiotic components, 8-12 parts of plant extracts, 3-5 parts of emulsification aids, 20-30 parts of deionized water and 15-20 parts of vegetable oil;

the preparation method comprises the following steps:

a1, firstly mixing functional oligosaccharide with deionized water according to corresponding parts by weight, and preheating at 40-60 ℃ to obtain a phase A;

a2, mixing the probiotic components, the plant extracts and the vegetable oil according to the corresponding parts by weight at 30-40 ℃ to obtain a phase B;

a3, after the A, B phase is cooled to room temperature under vacuum condition, mixing A, B phases, adding an emulsifying aid, and finally carrying out ultrasonic mixing to obtain the prebiotic composition.

By adopting the technical scheme, after the face cream is coated on the skin, the improvement of the skin surface microecology is realized under the compound effect of the prebiotic-functional oligosaccharide, the implanted probiotic-probiotic component and the plant extract, and compared with the single use of the prebiotic component, the face cream is favorable for assisting the probiotic to replace harmful bacteria, so that the probiotic occupies the dominant position of the skin surface microecology;

The obtained prebiotic composition is bicontinuous emulsion, and can form a stable microemulsion system under the action of an emulsification auxiliary agent and ultrasonic conditions, wherein the microemulsion system can wrap the probiotic components on the opposite inner sides of the microemulsion, and the functional oligosaccharides and the plant extracts are distributed on the opposite outer sides of the microemulsion system, so that the loss and activity loss of the functional oligosaccharides and the plant extracts are reduced while the activity of the probiotic components is effectively ensured.

Preferably, the functional oligosaccharide is one or more of fructo-oligosaccharide, xylo-oligosaccharide, galacto-oligosaccharide, isomalto-oligosaccharide, gentio-oligosaccharide, soy oligosaccharide and chitosan oligosaccharide.

By adopting the technical scheme, the functional oligosaccharide consisting of one or more of fructo-oligosaccharide, xylo-oligosaccharide, galacto-oligosaccharide, isomaltooligosaccharide, gentiooligosaccharide, soybean oligosaccharide and chitosan oligosaccharide can be used as basic nutrition supply, so that the growth of probiotics is ensured;

after the emulsion with double continuous phases is prepared, the effect of assisting in forming micro-emulsion can be achieved, the separation and protection effects on the probiotic components are achieved, and meanwhile, the emulsion can also achieve the effects of assisting in forming films and wetting protection after being coated on skin, so that the influence and interference of external adverse factors on probiotics are reduced.

Preferably, the probiotic component is compounded by one or more of lactobacillus gasseri, lactobacillus rhamnosus, bifidobacterium animalis, staphylococcus epidermidis, yeast cell lysate and lactobacillus plantarum lysate.

Preferably, the probiotic component consists of lactobacillus gasseri, bifidobacterium animalis, staphylococcus epidermidis and lactobacillus plantarum lysate in a weight ratio of 1 (3-5): 10-20): 8-15.

By adopting the technical scheme, the probiotic component compounded by lactobacillus gasseri, bifidobacterium animalis, staphylococcus epidermidis, yeast cell lysate and lactobacillus plantarum lysate can be used as an implanted external beneficial bacterium, so that the probiotic can replace harmful bacteria, occupy the dominant position of skin surface microecology, and can also obviously improve the living environment of epidermidis;

in addition, lactobacillus gasseri and bifidobacterium animalis used in the application are anaerobic bacteria, and enter injury parts (anaerobic environment and competition with harmful bacteria) such as acne under the action of bicontinuous emulsion, so that the propagation and growth environment of the harmful bacteria is destroyed, and the aim of inhibiting pathogens or the fixed value of the harmful bacteria is fulfilled; and the reproduction and development of staphylococcus epidermidis on the epidermis are not easily affected.

Preferably, the lactobacillus plantarum lysate extraction and lysis method is as follows:

b1, inoculating a slant culture of lactobacillus plantarum CCTCC NO: M2017399 into a seed culture medium, namely an MRS culture medium, and culturing for 8-12 hours under the anaerobic condition of 28-32 ℃ to obtain seed liquid;

b2, transferring the seed liquid well grown in the B1 into a fermentation culture medium according to the inoculum size of 0.5-1.2%, and culturing for 24-36h under the same condition of the B1 to obtain fermentation liquor;

the fermentation culture medium consists of MRS culture medium, cow milk and rye beer in the weight ratio of 1 (0.1-0.3) (0.05-0.10);

b3, centrifuging the fermentation liquor obtained in the step B2 at 8000-12000rpm for 20-30min, and taking supernatant to obtain lactobacillus plantarum lysate, and freeze-drying at-20 ℃ for later use.

By adopting the technical scheme, the lactobacillus plantarum lysate prepared by the extraction process has high content of beneficial proteins, amino acids and other nutrient substances in the lactobacillus plantarum lysate, has remarkable growth promoting effect on the lactobacillus gasseri of the same genus, can inhibit pathogens or harmful bacteria fixed values through the lactobacillus gasseri, and is compounded with forward implantation of staphylococcus epidermidis, so that the purpose that the probiotics replace harmful bacteria and occupy the microecological dominant position on the surface of skin is achieved.

In addition, it is to be noted that: the culture medium used in the application is a special culture medium and is compounded from MRS culture medium, cow milk and rye beer, the adjustment can effectively increase the protein and amino acid content in the final cracking product, and the addition of the rye beer can effectively protect the protein containing sulfhydryl from being oxidized, so that the activity of the obtained protein and amino acid is higher.

Preferably, the plant extract is prepared from one or more of red bean pulp, haematococcus, rehmannia root, walnut kernel, perilla, astragalus sinicus, peach petal, sesbania and radix asparagi.

Preferably, the plant extract is prepared from the following raw materials in parts by weight:

3-5 parts of soybean meal, 5-8 parts of haematococcus, 8-15 parts of rehmannia, 5-8 parts of perilla, 3-5 parts of peach petals, 3-5 parts of sesbania and 8-10 parts of radix asparagi.

By adopting the technical scheme, the plant extract extracted from the raw material components can be compounded with the functional oligosaccharide and the probiotics component to realize the improvement of the microecology on the skin surface, has the epidermis and acne repairing effect and the antibacterial effect, and can effectively destroy the living environment of anaerobic harmful bacteria, thereby promoting the probiotics to quickly replace the harmful bacteria and occupying the dominant position of the microecology on the skin surface.

Preferably, the extraction method of the plant extract comprises the following steps:

c1, cleaning and crushing the preparation raw materials of the plant extract, placing the plant extract in distilled water with 3-5 times of volume, performing aerobic fermentation at the pH of 5.0-6.0 and the temperature of 46-52 ℃ for 60-120min, and performing solid-liquid separation on fermentation liquor, and respectively refrigerating filtrate and filter residue for later use;

placing the filter residue obtained in the step C1 into an extracting solution with the volume of 1-2 times, adding cellulase accounting for 2-5%o of the extracting solution and pectase accounting for 1-3%o of the extracting solution, performing anaerobic fermentation for 4-8 hours at the temperature of 40-52 ℃ at the pH of 7-8, performing filter pressing for solid-liquid separation, removing the filter residue, combining the filtrate in the step C1, and performing vacuum distillation for water removal to obtain a plant extract;

wherein the extracting solution in the C2 consists of ethanol, polyglycerol-5 trioleate and ethyl acetate according to the weight ratio of 1 (0.1-0.3) to 0.3-0.5.

By adopting the technical scheme, the plant extract extracted by the process has simple preparation method, the final finished product does not need to remove the extracting solution, the content of effective active substances in the plant extract is high, and the plant extract can be compounded with functional oligosaccharide and probiotics to realize improvement of skin surface microecology and effectively destroy living environment of anaerobic harmful bacteria;

The analysis of the reasons may be two, on the one hand, since the C2 extracting solution is a multiphase extracting solution, the hydrophilic and lipophilic of the effective active substances can be respectively extracted; the second aspect is that the plant extract is treated by adopting a fermentation mode of firstly short-time aerobic and then long-time anaerobic, which provides a good decomposition environment for enzymes and additional enzymes of plants, thereby promoting the dissolution of relevant effective components in plant raw materials.

Preferably, the vegetable oil is one or more of shea butter, sweet almond oil, grape seed oil, sunflower seed oil, white pool seed oil and linseed oil.

By adopting the technical scheme, the vegetable oil compounded by the components can assist the formation of a micro-emulsion system of the bicontinuous phase, so that the vegetable oil can stably store functional substances such as functional oligosaccharide, probiotic components, plant extracts and the like, has a promoting effect on the epidermis of the plant extracts and the repairing and antibacterial effects of acnes, and each component is selected from green natural vegetable oil.

In a second aspect, the present application provides a method for preparing a prebiotic cream, which adopts the following technical scheme:

a preparation method of prebiotic cream comprises the following preparation steps:

Vacuum pouring the prebiotic composition and matrix formulation into emulsifying pot, homogenizing at normal temperature for 5-10min to obtain the final product.

By adopting the technical scheme, the preparation process of the face cream is obviously simplified, special conditions such as heating are not needed, the influence of temperature operation on the performance of the face cream and the prebiotic composition in the face cream is reduced, the performance of the obtained prebiotic face cream is stable and uniform, and the face cream has excellent moisturizing and conditioning effects, so that the face cream has extremely high economic benefit and is suitable for industrialized large-scale production.

In summary, the present application has the following beneficial effects:

1. the improvement effect of the microbial agent on the skin surface is realized by compounding the functional oligosaccharide, the probiotic component and the plant extract, which is beneficial to helping the probiotics to replace harmful bacteria and occupy the dominant position of the microbial agent on the skin surface, so that hidden danger caused by unbalanced flora on the skin surface is reduced;

2. the prebiotic composition exists in the form of bicontinuous emulsion, the emulsion can wrap the probiotic components on the opposite inner sides of the microemulsion, and the functional oligosaccharides and the plant extracts are distributed on the opposite outer sides of the microemulsion system, so that the loss and activity loss of the functional oligosaccharides and the plant extracts are reduced while the activity of the probiotic components is effectively ensured;

3. The probiotic component compounded by lactobacillus gasseri, bifidobacterium animalis, staphylococcus epidermidis, yeast cell lysate and lactobacillus plantarum lysate can be used as an implanted external beneficial bacterium, so that the probiotic can replace harmful bacteria, occupy the dominant position of the microecology on the surface of the skin, and can obviously improve the living environment of epidermidis so as to be unfavorable for the field planting of the harmful bacteria;

4. the preparation process is simplified, heating and other special conditions are not needed, the influence of temperature conditions on the performance of the face cream and the prebiotic composition in the face cream is reduced, and the obtained prebiotic face cream has stable and uniform performance and excellent skin moisturizing and conditioning effects, so that the face cream has extremely high economic benefit and is suitable for industrialized large-scale production.

Detailed Description

The present application will be described in further detail with reference to examples, wherein the raw materials used in the preparation examples and examples of the present application are commercially available common raw materials except for the following specific description;

lactobacillus with a preservation number of CGMCC No.6361;

bifidobacterium animalis with a preservation number of CGMCC No.12942;

staphylococcus epidermidis accession No. ATCC12228.

Preparation example

Preparation examples 1 to 5

The plant extract is prepared from the following raw materials in parts by weight:

c1, cleaning and crushing the preparation raw materials of the plant extract, placing the plant extract in distilled water with 3 times of volume, performing aerobic fermentation for 180min at the pH of 5.0 and the temperature of 46 ℃, and performing solid-liquid separation on fermentation liquor, wherein filtrate and filter residue are respectively refrigerated for later use;

c2, placing filter residues obtained in the step C1 into an extracting solution with the volume being 1 times, adding cellulase accounting for 3 permillage and pectase accounting for 2 permillage of the extracting solution, performing anaerobic fermentation for 12 hours under the conditions of pH 7.0 and the temperature of 40 ℃, performing filter pressing for solid-liquid separation, discarding filter residues, combining filtrate in the step C1, and performing vacuum distillation for water removal to obtain a plant extract;

the extracting solution in C2 consists of ethanol, polyglycerol-5 trioleate and ethyl acetate according to the weight ratio of 1:0.2:0.2.

Table: each component of the raw materials and its weight (kg) were extracted in production examples 1 to 5

Preparation example 6

The plant extract differs from preparation example 1 in that the specific preparation steps are as follows:

c1, cleaning and crushing the preparation raw materials of the plant extract, placing the plant extract in distilled water with 3 times of volume, performing aerobic fermentation for 120min at the pH of 5.0 and the temperature of 46 ℃, and performing solid-liquid separation on the fermentation liquid, wherein filtrate and filter residues are respectively refrigerated for later use;

And C2, placing filter residues obtained in the step C1 into an extracting solution with the volume being 1, adding cellulase accounting for 3 per mill of the extracting solution and pectase accounting for 2 per mill of the extracting solution, performing anaerobic fermentation for 8 hours at the pH of 7.0 and the temperature of 40 ℃, performing filter pressing for solid-liquid separation, discarding filter residues, combining filtrate in the step C1, and performing vacuum distillation for water removal to obtain the plant extract.

Preparation example 7

c1, cleaning and crushing the preparation raw materials of the plant extract, placing the plant extract in distilled water with 3 times of volume, performing aerobic fermentation for 100min at the pH of 5.5 and the temperature of 48 ℃, and performing solid-liquid separation on fermentation liquor, wherein filtrate and filter residue are respectively refrigerated for later use;

and C2, placing filter residues obtained in the step C1 into an extracting solution with the volume being 1, adding cellulase accounting for 3 per mill of the extracting solution and pectase accounting for 2 per mill of the extracting solution, performing anaerobic fermentation for 6 hours at the pH of 7.5 and the temperature of 46 ℃, performing filter pressing for solid-liquid separation, discarding filter residues, combining filtrate in the step C1, and performing vacuum distillation for water removal to obtain the plant extract.

Preparation example 8

C1, cleaning and crushing the preparation raw materials of the plant extract, placing the plant extract in distilled water with 3 times of volume, performing aerobic fermentation for 60min at the pH of 6.0 and the temperature of 52 ℃, and performing solid-liquid separation on fermentation liquor, wherein filtrate and filter residue are respectively refrigerated for later use;

and C2, placing filter residues obtained in the step C1 into an extracting solution with the volume being 1, adding cellulase accounting for 3 per mill of the extracting solution and pectase accounting for 2 per mill of the extracting solution, performing anaerobic fermentation for 4 hours at the pH value of 8.0 and the temperature of 52 ℃, performing filter pressing for solid-liquid separation, discarding filter residues, combining filtrate in the step C1, and performing vacuum distillation for water removal to obtain the plant extract.

Preparation example 9

A plant extract is different from preparation example 1 in that the extract in C2 consists of ethanol, polyglycerol-5 trioleate and ethyl acetate according to the weight ratio of 1:0.1:0.3.

Preparation example 10

A plant extract is different from preparation example 1 in that the extract in C2 consists of ethanol, polyglycerol-5 trioleate and ethyl acetate in a weight ratio of 1:0.3:0.5.

PREPARATION EXAMPLE 11

A lactobacillus plantarum lysate is prepared by the following steps:

b1, inoculating a slant culture of lactobacillus plantarum CCTCC NO: M2017399 into a seed culture medium, namely an MRS culture medium, and culturing for 12 hours under the anaerobic condition at 32 ℃ to obtain seed liquid;

B2, transferring the seed liquid well grown in the B1 into a fermentation culture medium according to the inoculation amount of 1.0%, and culturing for 30 hours under the same condition of the B1 to obtain a fermentation liquid;

the fermentation medium consists of MRS medium and cow milk in the weight ratio of 1:0.1;

and B3, centrifuging the fermentation liquor obtained in the step B2 at a rotating speed of 10000rpm for 25min, and taking supernatant to obtain lactobacillus plantarum lysate, and freeze-drying at-20 ℃ for later use.

Preparation example 12

Lactobacillus plantarum lysate differs from preparation 11 in that the lysis method is as follows:

b1, inoculating a slant culture of lactobacillus plantarum CCTCC NO: M2017399 into a seed culture medium, namely an MRS culture medium, and culturing for 12 hours under the anaerobic condition at 28 ℃ to obtain seed liquid;

Preparation example 13

b1, inoculating a slant culture of lactobacillus plantarum CCTCC NO: M2017399 into a seed culture medium, namely an MRS culture medium, and culturing for 8 hours under the anaerobic condition at 32 ℃ to obtain seed liquid;

B2, transferring the seed liquid well grown in the B1 into a fermentation culture medium according to the inoculation amount of 1.2%, and culturing for 24 hours under the same condition of the B1 to obtain fermentation liquid;

PREPARATION EXAMPLE 14

Lactobacillus plantarum lysate differs from preparation 11 in that the fermentation medium consists of MRS medium, cow's milk and rye beer (3.5 degrees) in a weight ratio of 1:0.1:0.05.

Preparation example 15

Lactobacillus plantarum lysate differs from preparation 11 in that the fermentation medium consists of MRS medium, cow's milk and rye beer (3.5 degrees) in a weight ratio of 1:0.2:0.10.

PREPARATION EXAMPLE 16

Lactobacillus plantarum lysate differs from preparation 11 in that the fermentation medium consists of MRS medium, cow's milk and rye beer (3.5 ℃) in a weight ratio of 1:0.3:0.10.

Preparation example 17

Lactobacillus plantarum lysate differs from preparation 11 in that the fermentation medium consists of MRS medium, cow's milk and rye beer (3.5 ℃) in a weight ratio of 1:0.3:0.20.

PREPARATION EXAMPLE 18

A lactobacillus plantarum lysate is different from preparation example 11 in that the preservation number of lactobacillus plantarum is CGMCC No.11156.

Preparation examples 19 to 26

A prebiotic composition, the components and their respective weights are shown in the following table and is prepared by the following method:

a1, firstly mixing functional oligosaccharide with deionized water at 800r/min for 30min, and preheating at 40 ℃ to obtain a phase A; wherein the functional oligosaccharide consists of fructo-oligosaccharide, chitosan oligosaccharide and isomaltooligosaccharide according to the weight ratio of 1:0.2:1.5;

a2, mixing the probiotic component, the plant extract and the vegetable oil at 30 ℃ for 10min at a rotating speed of 2000r/min to obtain a phase B;

wherein the probiotic component consists of lactobacillus gasseri, bifidobacterium animalis and staphylococcus epidermidis according to the weight ratio of 1:5:8;

the plant extract was prepared in preparation example 1;

the vegetable oil consists of shea butter and grape seed oil according to the weight ratio of 1:2;

a3, after the A, B phase is cooled to room temperature (25 ℃) under vacuum condition, premixing the A phase and the B phase for 5min at 2000r/min, adding an emulsifying aid, and finally carrying out ultrasonic mixing for 20min at the power of 2.0KW and the amplitude of 70um, thus obtaining the prebiotic composition.

Table: the prebiotic composition components and weights (kg) in preparation examples 19-26

Preparation example 27

A prebiotic composition differs from preparation 19 in that the functional oligosaccharide consists of fructo-oligosaccharide, galacto-oligosaccharide, isomalto-oligosaccharide and isomalto-oligosaccharide in a weight ratio of 1:1:0.5:0.3.

PREPARATION EXAMPLE 28

A prebiotic composition differs from preparation 19 in that the functional oligosaccharide consists of fructo-oligosaccharide, xylo-oligosaccharide and isomaltooligosaccharide in a weight ratio of 1:1.2:0.5.

Preparation example 29

A prebiotic composition differs from preparation 19 in that the probiotic composition consists of Lactobacillus gasseri, bifidobacterium animalis, staphylococcus epidermidis and Lactobacillus plantarum lysate obtained in preparation 11 in a weight ratio of 1:3:10:8.

Preparation example 30

A prebiotic composition differs from preparation 19 in that the probiotic composition consists of Lactobacillus gasseri, bifidobacterium animalis, staphylococcus epidermidis and Lactobacillus plantarum lysate obtained in preparation 11 in a weight ratio of 1:4:12:12.

Preparation example 31

A prebiotic composition differs from preparation 19 in that the probiotic composition consists of Lactobacillus gasseri, bifidobacterium animalis, staphylococcus epidermidis and Lactobacillus plantarum lysate obtained in preparation 11 in a weight ratio of 1:5:20:15.

PREPARATION EXAMPLE 32

A prebiotic composition differs from preparation 19 in that the probiotic composition consists of Lactobacillus gasseri, bifidobacterium animalis, staphylococcus epidermidis and Lactobacillus plantarum lysate obtained in preparation 11 in a weight ratio of 1:5:5:5.

PREPARATION EXAMPLES 33 to 40

A prebiotic composition differs from preparation 29 in that the Lactobacillus plantarum lysate used in the composition is used in a manner as shown in the following Table.

Table: table for comparing the use cases of Lactobacillus plantarum lysates in preparation examples 33-40

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PREPARATION EXAMPLES 41 to 49

A prebiotic composition differs from that of preparation 19 in that the plant extracts used in the components are used differently, and the specific correspondence is shown in the following table.

Table: table comparing usage of plant extracts in preparation examples 41 to 49

Group of	Plant extracts
		PREPARATION EXAMPLE 41	Prepared from preparation example 2
PREPARATION EXAMPLE 42	Prepared from preparation example 3
		Preparation example 43	Prepared from preparation 4
PREPARATION EXAMPLE 44	Prepared from preparation 5
		PREPARATION EXAMPLE 45	From preparation 6
Preparation example 46	Prepared from preparation 7
		Preparation example 47	Prepared from preparation 8
Preparation example 48	Prepared in preparation example 9
		Preparation example 49	Prepared from preparation 10

PREPARATION EXAMPLE 50

A prebiotic composition differs from preparation 19 in that the vegetable fat consists of shea butter, grape seed oil and white pond flower seed oil in a weight ratio of 1:2:0.5.

Performance test

The prebiotic creams (100 g/bottle) prepared in each example and comparative example were selected for detection, the subjects were 440 adults aged 25-50 years, 10 persons per group, and the subjects were required to clean the face before applying the prebiotic cream, and the water content of the face was kept at not higher than 20%;

Then, the skin harmful bacteria inhibition rate delta Q% is respectively detected after the application of the skin harmful bacteria inhibition rate delta Q% is carried out three times a day and 1+/-0.2 g each time, and the specific detection steps and reference standards are as follows:

skin pest inhibition rate Δq%:

the face is sampled before test, on the day of smearing and after 30 days of continuous smearing respectively, namely sterile cotton swab soaked by physiological saline is pressed on the face, the sterile cotton swab and the wiping surface are 45 degrees, and then the face is wiped (sampling area is 4cm ² ) About 30s, and preparing 10ml of sample solution by using normal saline;

then calculating the total number of bacterial colonies in each sample liquid by adopting a plate counting method, and obtaining the inhibition rate delta Q of the skin flora according to the ratio of the total number of the bacterial colonies after coating to the total number of the bacterial colonies before coating, so as to represent the improvement effect of the face cream on the microecology of the skin surface; the skin harmful bacteria are exemplified by staphylococcus aureus and propionibacterium acnes, and the detection results and the evaluation criteria are as follows:

and (3) the following materials: 60< Δq value (considered significant inhibition);

o: 10< Δq <60 (considered inhibitory);

x: Δq <10 (considered to be essentially non-inhibitory).

It should be noted that the main person of the tested person is a person who is a department of me, and after 30 days of continuous coating, the tested person does not return to the test, and the data of the tested person is discarded.

Examples

Examples 1 to 6

The prebiotic cream comprises the following components and the corresponding weights, and is prepared by the following preparation method;

vacuum pouring the prebiotic composition and matrix formulation into 4Kw emulsifying pot, homogenizing at 25deg.C under stirring speed of 80rpm for 10min to obtain the final product.

Wherein the prebiotic composition was prepared from preparation 19.

Table: each component in examples 1 to 6 and its weight (kg)

Comparative example 1

A prebiotic cream differs from example 1 in that the prebiotic composition is only a functional oligosaccharide.

Comparative example 2

A prebiotic cream differs from example 1 in that the prebiotic composition is the only probiotic component.

Comparative example 3

A prebiotic cream differs from example 1 in that the prebiotic composition is only a plant extract.

Comparative example 4

A prebiotic cream, different from example 1, was prepared by mixing the three components at 2000r/min, with the prebiotic composition comprising only functional oligosaccharides, probiotic components and plant extracts.

The prebiotic creams of examples 1-6 and comparative examples 1-4 were extracted and tested for their skin pest inhibition ΔQ according to the above measurement procedure and measurement criteria, and the average value of the test results is shown in the following table.

Table: results of Performance test of skin harmful bacteria inhibition RaQ% of examples 1 to 6 and comparative examples 1 to 4

From the above table, it can be seen that the prebiotic creams prepared in examples 1-6 all have excellent effect of improving skin surface microecology, and the purpose that probiotics occupy the dominant position of skin surface microecology can be achieved by inhibiting harmful bacteria and assisting probiotics to replace harmful bacteria;

the inhibition rate delta Q% of staphylococcus aureus on the day of smearing is 32.8-43.8%, and the inhibition rate delta Q% of propionibacterium acnes is 41.2-46.6%, which are regarded as having inhibition effect;

the inhibition rate delta Q% of staphylococcus aureus is 61.7-82.3% and the inhibition rate delta Q% of propionibacterium acnes is 79.1-89.5% after being continuously smeared for 30 days, and the composition is considered to have obvious inhibition effect.

The improvement of the microecology on the skin surface can be effectively realized through the compounding of the prebiotic-functional oligosaccharide, the implanted probiotic-probiotic component and the plant extract, and compared with the single use of the prebiotic component, the method reduces the risk of further propagation of harmful bacteria caused by unbalanced bacterial colony and ingestion of the prebiotic by the harmful bacteria and generation of vicious circulation.

In addition, as can be seen from the data in examples 1 to 6 of the above table, as the usage amount of the prebiotic composition increases, the effect of improving the microecology on the skin surface increases correspondingly, and when the usage amount of the prebiotic composition is 30 to 50%, the effect of the prebiotic composition approaches saturation, and the usage amount is continuously increased, so that the effect of the prebiotic composition is slightly improved, thus combining the actual production cost and effect, and taking examples 4 to 5 as preferred examples.

Further, as can be seen from comparative examples 1 to 3, the effect of improving the microecology on the skin surface is reduced to different degrees when any one of the prebiotic-functional oligosaccharide, the implanted probiotic-probiotic component and the plant extract is absent; wherein the improvement effect on the skin surface micro-ecology is substantially lost in comparative example 1:

the inhibition rate delta Q% of staphylococcus aureus on the day of smearing is 8.1 percent, the inhibition rate delta Q% of propionibacterium acnes is 6.5 percent, and no inhibition effect is considered;

the inhibition rate DeltaQ% of staphylococcus aureus was 7.8% and the inhibition rate DeltaQ% of propionibacterium acnes was 6.1% after continuous application for 30 days, and no inhibition effect was considered.

Comparative examples 2 to 3 only have a certain effect of improving the microecology of the skin surface, and comparative example 3 has better effect of long-term application: the inhibition rate delta Q% of staphylococcus aureus on the day of smearing is 11.2-17.2% and the inhibition rate delta Q% of propionibacterium acnes is 18.3-24.5%, which is regarded as having a certain inhibition effect;

the inhibition rate delta Q% of staphylococcus aureus is 25.8-31.2% and the inhibition rate delta Q% of propionibacterium acnes is 38.2-43.2% after being continuously smeared for 30 days, and the composition has a certain inhibition effect.

Further, as can be seen from comparative example 4, the level of inhibition on the day of application was comparable to that of example 1, but the inhibition rate Δq% of staphylococcus aureus on 30 days of application was only 42.2%, and the inhibition rate Δq% of propionibacterium acnes was only 52.4%, all of which were reduced to different degrees.

It can be seen that only a specific bicontinuous phase emulsion system can ensure the stable improvement effect on the skin surface micro-ecology, and has better use effect compared with the conventional water-in-oil emulsion and oil-in-water emulsion;

the reason for this may be analyzed as follows: functional oligosaccharides are generally hydrophilic, while probiotic components have hydrophobicity and hydrophilicity respectively due to the inclusion of both living bacteria and cleavage products, and the inner components have both hydrophobicity and hydrophilicity as in plant extracts;

therefore, the separation effect of the water-in-oil emulsion and the oil-in-water emulsion on the components is limited, when the prebiotics are contacted with probiotics, a large amount of consumption of a single component is caused, even the original compounding effect of the component is lost due to the large consumption of the component, the bicontinuous phase emulsion obviously does not have the defects, and even if the loss of part of the components occurs, the loss amount is balanced and small, and the compounding effect is not influenced due to the characteristics of hydrophilic and oleophilic properties and disorder of the two sides of the bicontinuous phase emulsion.

Secondly, the cream is thicker in texture and is suitable for dry skin, so that only a water-in-oil system can be adopted, but according to the ratio of hydrophilic and lipophilic components in the cream, more hydrophilic components in the cream can be found, and the cream is obviously not suitable for the water-in-oil system.

Examples 7 to 13

The prebiotic cream differs from example 1 in the use of the prebiotic composition used, the specific correspondence being shown in the following table.

Table: table comparing usage of prebiotic compositions in examples 7-13

Group of	Prebiotic composition
		Example 7	From preparation example 20
Example 8	Prepared from preparation 21
		Example 9	From preparation 22
Example 10	From preparation 23
		Examples11	From preparation 24
Example 12	From preparation 25
		Example 13	From preparation 26

The prebiotic creams of examples 7-13 were extracted and tested for their skin pest inhibition Δq according to the above-described measurement procedure and measurement criteria, and the average of the test results is reported in the following table.

Table: examples 7 to 13 results of Performance test for skin harmful bacteria inhibition RaQ%

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From the above table, it can be seen that the prebiotic creams prepared in examples 1 and 7-13 have excellent effect of improving skin surface microecology, and the purpose that probiotics occupy the dominant position of skin surface microecology can be achieved by inhibiting harmful bacteria and assisting probiotics to replace harmful bacteria;

the inhibition rate delta Q% of staphylococcus aureus on the day of smearing is 32.8-34.2%, and the inhibition rate delta Q% of propionibacterium acnes is 41.2-45.3%, which are regarded as having inhibition effect;

The inhibition rate delta Q% of staphylococcus aureus is 61.7-73.3% and the inhibition rate delta Q% of propionibacterium acnes is 79.1-88.8% after being continuously smeared for 30 days, and the composition is considered to have obvious inhibition effect.

Therefore, under the condition that the total dosage of the prebiotic components is certain, the improvement effect on the microecology of the skin surface can be enhanced by adjusting the proportion of each component, and the addition of the functional oligosaccharide, the probiotic components and the plant extract can strengthen the inhibition effect on harmful bacteria by combining the data in the table, and the effect on the performance is less because the components tend to be saturated after reaching a certain amount.

Examples 14 to 15

Table: table comparing usage of prebiotic compositions in examples 14-15

Group of	Prebiotic composition
		Example 14	From preparation 27
Example 15	From preparation 28

The prebiotic creams of examples 14-15 above were extracted and tested for their skin pest inhibition Δq according to the above-described measurement procedure and measurement criteria, and the average of the test results is reported in the following table.

Table: examples 14 to 15 results of Performance test for skin harmful bacteria inhibition RaQ%

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From the above table, it can be seen that the prebiotic creams prepared in examples 1 and 14-15 all have excellent effect of improving skin surface microecology, and the purpose that probiotics occupy the dominant position of skin surface microecology can be achieved by inhibiting harmful bacteria and assisting probiotics to replace harmful bacteria;

the inhibition rate delta Q% of staphylococcus aureus on the day of smearing is 32.6-33.0%, and the inhibition rate delta Q% of propionibacterium acnes is 41.2-41.5%, which are regarded as having inhibition effect;

the inhibition rate delta Q% of staphylococcus aureus is 61.4-62.1% and the inhibition rate delta Q% of propionibacterium acnes is 79.1-79.8% after being continuously smeared for 30 days, and the composition is considered to have obvious inhibition effect.

It can be seen that the effect of the selection of the functional oligosaccharides on the actual performance is weak and can be ignored under the condition that the total amount of the prebiotic components and other components are not changed, but the more the types of the functional oligosaccharides, the better the supply effect is, and the growth of the probiotics can be preferably ensured.

In addition, after the emulsion with double continuous phases is prepared, the functions of assisting in forming micro-emulsion can be achieved, the separation and protection functions of the probiotic components are achieved, and meanwhile, the effects of assisting in film forming and moistening protection can be achieved after the emulsion is coated on skin, so that the influence and interference of external adverse factors on probiotics are reduced.

Examples 16 to 19

Table: table for comparing usage of prebiotic compositions in examples 16-19

The prebiotic creams of examples 16-19 above were extracted and tested for their skin pest inhibition Δq according to the above-described measurement procedure and measurement criteria, and the average of the test results is reported in the following table.

Table: examples 16 to 19 results of Performance test for skin harmful bacteria inhibition RaQ%

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From the above table, it can be seen that the prebiotic creams prepared in examples 1 and 16-19 all have excellent effect of improving skin surface microecology, and the purpose that probiotics occupy the dominant position of skin surface microecology can be achieved by inhibiting harmful bacteria and assisting probiotics to replace harmful bacteria;

the inhibition rate delta Q% of staphylococcus aureus on the day of smearing is 32.8-35.7%, and the inhibition rate delta Q% of propionibacterium acnes is 41.2-48.8%, which are regarded as having inhibition effect;

the inhibition rate delta Q% of staphylococcus aureus is 61.7-71.0% and the inhibition rate delta Q% of propionibacterium acnes is 79.1-90.1% after being continuously smeared for 30 days, and the composition is considered to have obvious inhibition effect.

The probiotic component compounded by lactobacillus gasseri, bifidobacterium animalis, staphylococcus epidermidis and lactobacillus plantarum lysate can be used as an implanted external beneficial bacterium, so that the probiotic can replace harmful bacteria, occupy the dominant position of skin surface microecology, and can also obviously improve the living environment of epidermidis.

In addition, the lactobacillus gasseri and the bifidobacterium animalis are anaerobic bacteria, and enter injury parts such as acne and the like to compete with harmful bacteria under the action of bicontinuous emulsion, so that the propagation and growth environment of the harmful bacteria is destroyed, and the aim of inhibiting pathogens or the fixed value of the harmful bacteria is fulfilled; but not easily influence the reproduction and development of staphylococcus epidermidis in epidermis.

The mixed lactobacillus plantarum lysate can promote growth of lactobacillus rhamnosus of the same genus, inhibit fixed values of pathogens or harmful bacteria, and compound forward implantation of staphylococcus epidermidis, so that the purpose that probiotics replace harmful bacteria and occupy the microecological dominant position on the surface of skin is achieved.

Examples 20 to 27

The prebiotic cream differs from example 16 in the use of the prebiotic composition used, the specific correspondence being shown in the following table.

Table: table for comparing usage of prebiotic compositions in examples 20-27

Group of	Prebiotic composition
		Example 20	From preparation 33
Example 21	From preparation 34
		Example 22	From preparation 35
Example 23	From preparation 36
		Example 24	From preparation 37
Example 25	From preparation 38
		Example 26	From preparation 39
Example 27	From preparation 40

The prebiotic creams of examples 20-27 above were extracted and tested for their skin pest inhibition Δq according to the above-described measurement procedure and measurement criteria, and the average of the test results is reported in the following table.

Table: examples 20 to 27 results of Performance test for skin harmful bacteria inhibition RaQ%

From the above table, it can be seen that the prebiotic creams prepared in examples 16 and 20-27 all have excellent effect of improving skin surface microecology, and the purpose that probiotics occupy the dominant position of skin surface microecology can be achieved by inhibiting harmful bacteria and assisting probiotics to replace harmful bacteria;

the inhibition rate delta Q% of staphylococcus aureus on the day of smearing is 34.8-35.8%, and the inhibition rate delta Q% of propionibacterium acnes is 47.8-48.6%, which are regarded as having inhibition effect;

the inhibition rate delta Q% of staphylococcus aureus is 68.5-69.1% and the inhibition rate delta Q% of propionibacterium acnes is 86.8-88.1% after being continuously smeared for 30 days, and the composition is regarded as having obvious inhibition effect.

The lactobacillus plantarum lysate prepared by the extraction process has higher retention of beneficial proteins, amino acids and other nutrient substances, and can play a remarkable role in promoting the growth of strains;

in addition, as is apparent from examples 23-26, the culture medium compounded from MRS culture medium, cow's milk and rye beer can effectively increase the protein and amino acid content in the final lysate, and the reason for this analysis may be: the addition of the rye beer can effectively protect the protein containing sulfhydryl groups from being oxidized and part of the protein in the cow milk from being decomposed, so that the activity of the obtained protein and amino acid is higher.

Examples 28 to 36

Table: table comparing usage of prebiotic compositions in examples 28-36

Group of	Prebiotic composition
		Example 28	From preparation 41
Example 29	From preparation 42
		Example 30	From preparation 43
Example 31	From preparation 44
		Example 32	From preparation 45
Example 33	From preparation 46
		Example 34	From preparation 47
Example 35	From preparation 48
		Example 36	From preparation 49

Comparative example 5

The prebiotic cream differs from example 1 in that the plant extract used is prepared by the following method:

c1, cleaning and crushing the preparation raw materials of the plant extract, placing the plant extract in distilled water with 3 times of volume, performing anaerobic fermentation for 180min at the pH of 5.0 and the temperature of 46 ℃, and performing solid-liquid separation on the fermentation broth, wherein filtrate and filter residues are respectively refrigerated for later use; and C2, placing filter residues obtained in the step C1 into an extracting solution with the volume being 1, adding cellulase accounting for 3 per mill of the extracting solution and pectase accounting for 2 per mill of the extracting solution, continuously carrying out anaerobic fermentation for 12 hours under the conditions of pH 7.0 and the temperature of 40 ℃, carrying out filter pressing for solid-liquid separation, discarding the filter residues, combining the filter residues in the step C1, and carrying out vacuum distillation for water removal to obtain the plant extract.

The prebiotic creams of examples 28-36 and comparative example 5 above were drawn and tested for their skin pest inhibition ΔQ according to the above measurement procedure and measurement criteria, and the average of the test results is reported in the following table.

Table: results of Performance test of skin harmful bacteria inhibition RaQ% of examples 28 to 36, comparative example 5

From the above table, it can be seen that the prebiotic creams prepared in examples 1 and 28-36 have excellent effect of improving skin surface microecology, and the purpose that probiotics occupy the dominant position of skin surface microecology can be achieved by inhibiting harmful bacteria and assisting probiotics to replace harmful bacteria;

The inhibition rate delta Q% of staphylococcus aureus on the day of smearing is 32.4-35.5%, and the inhibition rate delta Q% of propionibacterium acnes is 41.0-47.1%, which are regarded as having inhibition effect;

the inhibition rate delta Q% of staphylococcus aureus is 61.1-67.8% and the inhibition rate delta Q% of propionibacterium acnes is 78.1-83.6% after being continuously smeared for 30 days, and the composition is considered to have obvious inhibition effect.

The plant extract extracted from the raw material components can be compounded with functional oligosaccharide and probiotics components to improve the microecology on the skin surface, has the epidermis and acne repairing effect and the antibacterial effect, and can effectively destroy the living environment of anaerobic harmful bacteria, so that probiotics are promoted to replace the harmful bacteria rapidly and occupy the dominant position of the microecology on the skin surface, and particularly, the embodiment 1 and the embodiment 28-31 are shown;

the plant extract extracted by the process has high content of effective active substances, can be compounded with functional oligosaccharide and probiotics components to improve the microecology on the skin surface, and can effectively destroy the living environment of anaerobic harmful bacteria; the reasons for analysis may be two, on the one hand, since the C2 extract is a multiphase extract, the respective extraction can be performed for the hydrophilicity and lipophilicity of the effective active substances, see examples 1, 35-36;

The second aspect is that the method adopts a fermentation mode of firstly short-time aerobic and then long-time anaerobic to treat the plant extract, and provides a good decomposition environment for enzymes and additional enzymes of plants, thereby promoting the dissolution of relevant effective components in plant raw materials, and the extraction effect is better than that of direct anaerobic fermentation, as shown in comparative example 5.

Example 37

A prebiotic cream differs from example 1 in that the prebiotic composition used was prepared from preparation 50, i.e. vegetable fat consisted of shea butter, grape seed oil and white pool seed oil in a weight ratio of 1:2:0.5.

Comparative example 6

A prebiotic cream differs from example 1 in that the prebiotic composition used does not contain vegetable fat.

The prebiotic creams of example 37 and comparative example 6 were extracted and tested for their skin pest inhibition Δq according to the above-described measurement procedure and measurement criteria, and the average value of the test results is shown in the following table.

Table: results of Performance test of skin harmful bacteria inhibition RaQ% in example 37 and comparative example 6

As can be seen from the above table, the prebiotic creams prepared in examples 1 and 37 have excellent effect of improving skin surface microecology, and can achieve the purpose that probiotics occupy the dominant position of skin surface microecology by inhibiting harmful bacteria and assisting probiotics to replace harmful bacteria;

The inhibition rate delta Q% of staphylococcus aureus on the day of smearing is 32.8%, and the inhibition rate delta Q% of propionibacterium acnes is 41.2-41.3%, which is regarded as having inhibition effect;

the inhibition rate delta Q% of staphylococcus aureus is 61.7-61.8% and the inhibition rate delta Q% of propionibacterium acnes is 79.1-79.3% after being continuously smeared for 30 days, and the composition is considered to have obvious inhibition effect.

The vegetable oil compounded by the components can assist the formation of a micro-emulsion system of a bicontinuous phase, so that the vegetable oil can stably store functional substances such as functional oligosaccharide, probiotic components, plant extracts and the like, and has certain promotion effects on the epidermis of the plant extracts and the repairing and antibacterial effects of acnes, and the comparison example 6 is provided.

The present embodiment is merely illustrative of the present application and is not intended to be limiting, and those skilled in the art, after having read the present specification, may make modifications to the present embodiment without creative contribution as required, but is protected by patent laws within the scope of the claims of the present application.

Claims

1. A prebiotic facial cream, characterized by comprising a prebiotic composition and a matrix formulation;

the preparation method comprises the following steps:

a3, after the A, B phase is cooled to room temperature under vacuum condition, mixing A, B phases, adding an emulsifying aid, and finally carrying out ultrasonic mixing to obtain the prebiotic composition;

wherein the probiotic components consist of lactobacillus gasseri, bifidobacterium animalis, staphylococcus epidermidis and lactobacillus plantarum lysate according to the weight ratio of 1 (3-5) (10-20) (8-15);

wherein the plant extract is prepared from the following raw materials in parts by weight:

3-5 parts of red bean pulp, 5-8 parts of haematococcus, 8-15 parts of rehmannia, 5-8 parts of perilla, 3-5 parts of peach petals, 3-5 parts of sesbania and 8-10 parts of radix asparagi;

the extraction method of the plant extract comprises the following steps:

wherein the extracting solution in the C2 consists of ethanol, polyglycerol-5 trioleate and ethyl acetate according to the weight ratio of 1 (0.1-0.3) (0.3-0.5);

wherein the vegetable oil is one or more of shea butter, sweet almond oil, grape seed oil, sunflower seed oil, white pool flower seed oil and linseed oil.

2. The prebiotic cream of claim 1 wherein the functional oligosaccharide is one or more of fructo-oligosaccharide, xylo-oligosaccharide, galacto-oligosaccharide, isomalto-oligosaccharide, gentio-oligosaccharide, soy-oligosaccharide and chitosan oligosaccharide.

3. The prebiotic cream of claim 1 wherein the lactobacillus plantarum lysate is extracted by the following method:

B2, transferring the seed liquid well grown in the B1 into a fermentation culture medium according to the inoculum size of 0.5-1.2%, and culturing 24-36 h under the same condition of the B1 to obtain a fermentation liquid;

b3, centrifuging the fermentation liquor obtained in the step B2 at 8000-12000 rpm for 20-30min, and taking supernatant to obtain lactobacillus plantarum lysate, and freeze-drying at-20 ℃ for later use.

4. A process for the preparation of a prebiotic cream according to any one of claims 1 to 3, characterised by the specific steps of: