CN116267594A - Device for promoting formation of rubber tree 2n gametes - Google Patents
Device for promoting formation of rubber tree 2n gametes Download PDFInfo
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- CN116267594A CN116267594A CN202310055200.6A CN202310055200A CN116267594A CN 116267594 A CN116267594 A CN 116267594A CN 202310055200 A CN202310055200 A CN 202310055200A CN 116267594 A CN116267594 A CN 116267594A
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- rubber tree
- temperature
- temperature control
- gametes
- air compressor
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- 244000043261 Hevea brasiliensis Species 0.000 title claims abstract description 48
- 230000001737 promoting effect Effects 0.000 title claims abstract description 15
- 230000015572 biosynthetic process Effects 0.000 title claims abstract description 12
- 239000006260 foam Substances 0.000 claims abstract description 22
- 238000010438 heat treatment Methods 0.000 claims description 8
- 239000000523 sample Substances 0.000 claims description 7
- 230000006543 gametophyte development Effects 0.000 claims 5
- 208000026487 Triploidy Diseases 0.000 abstract description 14
- 238000009395 breeding Methods 0.000 abstract description 7
- 230000001488 breeding effect Effects 0.000 abstract description 7
- 230000010152 pollination Effects 0.000 abstract description 6
- 238000011161 development Methods 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 3
- 208000020584 Polyploidy Diseases 0.000 description 10
- 238000011282 treatment Methods 0.000 description 5
- 230000005611 electricity Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 229920003052 natural elastomer Polymers 0.000 description 4
- 229920001194 natural rubber Polymers 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 230000037323 metabolic rate Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229930000044 secondary metabolite Natural products 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/09—Apparatus for producing changes in chromosome number
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- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a device for promoting formation of rubber tree 2n gametes, which comprises a distribution box, an air compressor, temperature control equipment and a foam box, wherein the distribution box is respectively connected with the air compressor and the temperature control equipment, the air compressor is connected with the temperature control equipment through an air pipe, and the temperature control equipment is connected with the foam box. The invention induces the flower buds of the rubber tree to generate 2n gametes through high temperature, low temperature or high temperature and low temperature staggering, ensures the normal growth and development of the rubber tree, and simultaneously generates the triploid (3 n) of the rubber tree after pollination of the 2n gametes and the normal n gametes. The method has great significance for breeding a new high-yield cold-resistant variety of the rubber tree triploid.
Description
Technical Field
The invention relates to the technical field of rubber tree breeding, in particular to a device for promoting formation of 2n gametes of rubber trees.
Background
Rubber tree is an important cash crop cultivated commercially in tropical zone, and its secondary metabolic substance, natural rubber, is one of four industrial raw materials, and is the only renewable raw material in the four industrial raw materials. And the natural rubber self-supporting rate in China is only about 20%, and the cultivation of the high-yield cold-resistant new variety is the only feasible way for improving the domestic natural rubber supply rate. Compared with diploid, polyploid plant has the characteristics of large cell volume, low metabolic rate, high secondary metabolite yield, fast early growth, large individual, wide and thick leaves, few branches, few flowers and fruits, large seeds, strong adaptability and the like, and has a plurality of advantages in production and application.
The polyploid breeding work of rubber trees is more studied at home and abroad, and starts to be studied as early as the 40 th century and also starts to be studied at the 70 th century in China. At present, the rubber tree polyploid plant is obtained through different ways of direct selection and separation in nature, living body induction, in vitro tissue cell induction, hybridization among different ploidy, hybridization of artificial 2n gametes and the like. However, there have been no reports of the application of artificial triploid varieties of rubber trees to production at home and abroad except for the natural triploid varieties of cloud-ground 77-2 and cloud-ground 77-4.
Stress, such as extreme low temperatures, tends to induce polyploidy. Polyploids tend to have higher viability than diploids, being more widely distributed in extreme environments such as high latitude, high altitude areas, and more numerous such as cloud cover 77-2 and cloud cover 77-4 cultivated in large areas in Yunnan and Guangdong. The stress is the motive power of plant polyploidy, can improve the occurrence probability of polyploidy and promote the formation of polyploidy. The mutation rate of the natural triploid of the rubber tree is extremely low and is only about 0.72 percent.
Aiming at the situation that the natural mutation rate of the gametes of the rubber tree 2n is extremely low, a device for promoting the formation of the gametes of the rubber tree 2n is specially designed, so that the flower buds of the rubber tree are in an artificial low-temperature, high-temperature and low-temperature staggered environment in a certain specific period of formation and differentiation, the chromosome numbers of megasporocyte of embryo sacs and microsporocyte of pollen are mutated, the normal n is changed into 2n, and then triploid (3 n) is formed through pollination. In order to meet the increasing demands of the nation on natural rubber and avoid variety degradation caused by long-term planting, the invention has great significance for breeding high-yield cold-resistant new varieties of the rubber tree triploid.
Disclosure of Invention
Aiming at the problems, the invention provides a device for promoting the formation of 2n gametes of the rubber tree, which has great significance for breeding high-yield new-cold-resistant varieties of rubber tree triploid.
According to the purpose of the invention, the device for promoting the formation of the gametes of the rubber tree 2n comprises a distribution box, an air compressor, temperature control equipment and a foam box, wherein the distribution box is respectively connected with the air compressor and the temperature control equipment, the air compressor is connected with the temperature control equipment through an air pipe, and the temperature control equipment is connected with the foam box.
Further, a refrigerating/heating device, a wireless hygrothermograph and a temperature sensor are arranged in the foam box.
Further, the air compressor is connected with the electric box through a power line.
Further, the outer diameter of the air pipe is 8mm, and the inner diameter of the air pipe is 5mm.
Further, the air pipe adopts a transparent air guide hose.
Further, the wireless hygrothermograph and the temperature sensor are connected with the temperature control equipment through a temperature control probe feedback line.
Further, the air compressor is connected with a programmable controller.
The invention is carried out by low temperature, high temperature or high temperature and low temperature interlacing to generate polyploid. The invention induces the production of 2n gametes when the rubber tree responds to extreme temperature stress. The method can ensure the normal growth and development of the rubber tree, and simultaneously explore the cold-resistant potential of the rubber tree, thereby having great significance for breeding new high-yield cold-resistant varieties of the rubber tree triploid.
Drawings
FIG. 1 is a schematic diagram of an embodiment of the present invention;
FIG. 2 is a schematic diagram of a temperature control apparatus according to an embodiment of the present invention;
fig. 3 is a schematic structural view of a foam tank according to an embodiment of the present invention.
In the figure, 1, a distribution box; 2. an air compressor; 3. a temperature control device; 4. a foam box; 5. an air pipe; 6. a switching power supply; 7. a temperature controller; 8. a pneumatic solenoid valve; 9. a time-controlled switch; 10. a cooling/heating device; 11. a wireless hygrothermograph; 12. a temperature sensor; 13. a feedback line; 14. and a power supply line.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described in the following in conjunction with the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
As shown in fig. 1, the device for promoting the formation of gametes of the rubber tree 2n comprises a distribution box 1, an air compressor 2, a temperature control device 3 and a foam box 4, wherein a switch, a socket, a time control switch and an alternating current contactor are arranged on the distribution box 1, the distribution box 1 is respectively connected with the air compressor 2 and the temperature control device 3, the air compressor 2 can select 220V or 380V voltage, the power is more than 3000W, the air compressor 2 is connected with the temperature control device 3 through an air pipe 5, and the air pipe 5 adopts a transparent air guide hose.
As shown in fig. 2, the temperature control device 3 includes a switching power supply 6, a temperature controller 7, a pneumatic solenoid valve 8, and a time-controlled switch 9. In the embodiment, the switching power supply 6 adopts a Ningbo Ming latitude NDR-240-24 guide rail type switching power supply, the temperature controller 7 adopts a bright control multipath guide rail type temperature controller DIN353 relay +RS485 communication, and the pneumatic electromagnetic valve 8 adopts a Advance 2V025-08-A pneumatic normally closed electromagnetic valve.
As shown in fig. 3, the temperature control device 3 is connected with a foam box 4, rubber tree flower buds are wrapped in the foam box 4, and a refrigerating/heating device 10, a wireless hygrothermograph 11 and a temperature sensor 12 are arranged in the foam box 4. The wireless hygrothermograph 11 and the temperature sensor 12 in the foam box 4 are connected with the temperature control device 3 through a temperature control probe feedback line 13. The refrigerating/heating device 10 adopts a low-temperature maxwell vortex tube of the refrigerating/heating device SDK 08W.
In the embodiment, the temperature treatment is carried out on the flower buds of the rubber tree under the conditions of convenient electricity utilization and base station signals. The air compressor machine 2 is connected with block terminal 1 through power cord 14, and wireless hygrothermograph 11 adopts the brand: the GSM501 wireless hygrothermograph can remotely observe the temperature and the humidity in real time. The outer diameter of the air pipe 5 is 8mm, and the inner diameter is 5mm.
The time switch 9 of the present embodiment sets the start and end times by the time switch. One setting can realize single and multiple control (such as three different time periods in a day, and a fixed time period in a week).
In this embodiment, a plurality of white foam boxes may be provided, each of which is provided with a high-temperature-resistant waterproof probe/20 m wire. In addition, an inserted type/20 meter probe wire/double waterproof probe rod digital display thermometer is used as a control.
In the embodiment, the temperature gradient and the time gradient are set, different temperature treatments are carried out on the flower buds of the rubber tree, the 2n gametes are induced to generate, and triploid is generated after pollination.
Example 2
The present embodiment is basically the same as embodiment 1 in structure, except that the present embodiment is applied to the case of conveniently using electricity without a base station signal. In the embodiment, the brand of the air compressor 2 is Sijingda, the model jsdkyj-01, 220V and 60L.
In the embodiment, the temperature gradient and the time gradient are set, the flower buds of the rubber tree are subjected to low-temperature treatment, 2n gametes are induced to generate triploid after pollination.
Example 3
The present embodiment has substantially the same structure as the above embodiment, except that the present embodiment employs a high-power air compressor, and further includes a paperless recorder (brand: glow control, model: THMA). In the embodiment, the foam box 4 adopts a low-temperature incubator, the low-temperature incubator is provided with a temperature sensor 12, and the temperature sensor 12 adopts a high-precision temperature sensor of a brightness control PT 100.
The embodiment is applied to the condition of convenient electricity utilization, and can set the temperature and the time through the setting, and when the embodiment is connected with a computer, the temperature real-time visualization is realized on a computer screen. The paperless recorder can also store half a year of temperature record without connecting with a computer. The device carries out temperature treatment on the flower buds of the rubber tree, induces 2n gametes, and generates triploid after pollination.
Example 4
The embodiment has basically the same structure as embodiment 3, and is different in that the embodiment is connected with a programmable controller under the condition of convenient electricity consumption, is programmed by an RS485 protocol (temperature and time are controlled), is converted into a USB interface (brand: glow control, model: RS 485), is used for carrying out temperature treatment on the flower buds of the rubber tree by programming the set temperature and time, and is used for inducing to generate 2n gametes and generating triploid by pollination.
In the above embodiment, various temperature modes may be set, such as: low temperature 8 ℃ for 6 hours; high temperature 44 deg.c for 9 hr; the temperature is 47 ℃ for 2 hours, and then the temperature is 7 ℃ for 4 hours; the temperature is firstly low for 3 hours at 6 ℃, and then the temperature is high for 3 hours at 45 ℃.
The invention is carried out by high temperature, low temperature or high temperature and low temperature interlacing, and the variation is created in the 3 modes to generate polyploid. The invention can also be remotely monitored by a computer and a mobile phone to see temperature and humidity data and record half-year temperature data.
The power distribution box 1 respectively provides proper power for the air compressor 2 and the temperature control equipment 3, controls the working time of the air compressor 2 through the time control switch and the alternating current contactor, is connected to the temperature control equipment 3 through the air pipe 5 after the air compressor 2 generates enough high-pressure air flow, and then controls the on and off of the pneumatic electromagnetic valve through the set temperature range, so that the high-pressure air flow sent by the air compressor 2 is controlled to be on or off, and then the high-pressure air flow controlled by the temperature control equipment 3 is sent into the foam box 4 through the air pipe and then is connected to the refrigerating/heating device in the foam box 4, and the high-pressure air flow generates cold air or hot air (switchable) after passing through the refrigerating/heating device, so that the air around the flower buds of the rubber tree wrapped in the foam box 4 is cooled or heated. The temperature probe in the foam box 4 collects temperature signals and then feeds the temperature signals back to the temperature control equipment through the feedback line 13 to realize accurate temperature control, and meanwhile, the wireless hygrothermograph in the foam box can also send temperature data to a mobile phone or a computer to realize remote observation and control.
The invention induces the production of 2n gametes when the rubber tree responds to extreme temperature stress. The method can ensure the normal growth and development of the rubber tree, and simultaneously explore the cold-resistant potential of the rubber tree, thereby having great significance for breeding new high-yield cold-resistant varieties of the rubber tree triploid.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.
Claims (7)
1. The device for promoting the formation of the rubber tree 2n gametes is characterized by comprising a distribution box, an air compressor, temperature control equipment and a foam box, wherein the distribution box is connected with the air compressor and the temperature control equipment respectively, the air compressor is connected with the temperature control equipment through an air pipe, and the temperature control equipment is connected with the foam box.
2. The device for promoting the formation of gametes of rubber tree 2n according to claim 1, wherein a refrigerating/heating device, a wireless hygrothermograph and a temperature sensor are arranged in the foam box.
3. The device for promoting gametogenesis of rubber tree 2n according to claim 1, wherein the air compressor is connected with an electric box through a power line.
4. The device for promoting gametogenesis of rubber tree 2n according to claim 1, wherein the outer diameter of the air tube is 8mm and the inner diameter of the air tube is 5mm.
5. The device for promoting gametogenesis of rubber tree 2n according to claim 1, wherein the air tube adopts a transparent air guide hose.
6. The apparatus for promoting gametogenesis of rubber tree 2n of claim 2, wherein the wireless hygrothermograph and the temperature sensor are connected to the temperature control device by a temperature control probe feedback line.
7. The device for promoting gametogenesis of rubber tree 2n of claim 1, wherein the air compressor is connected with a programmable controller.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310055200.6A CN116267594B (en) | 2023-02-04 | 2023-02-04 | Device for promoting formation of rubber tree 2n gametes |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310055200.6A CN116267594B (en) | 2023-02-04 | 2023-02-04 | Device for promoting formation of rubber tree 2n gametes |
Publications (2)
| Publication Number | Publication Date |
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| CN116267594A true CN116267594A (en) | 2023-06-23 |
| CN116267594B CN116267594B (en) | 2024-06-14 |
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| CN202310055200.6A Active CN116267594B (en) | 2023-02-04 | 2023-02-04 | Device for promoting formation of rubber tree 2n gametes |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1669408A (en) * | 2005-04-20 | 2005-09-21 | 北京林业大学 | High temperature duplicater for trees |
| WO2010034961A1 (en) * | 2008-09-23 | 2010-04-01 | Sumatra Biosciences Pte Ltd | Methods of obtaining hevea bras iliensis plants |
| US20140223813A1 (en) * | 2011-06-10 | 2014-08-14 | The Agency For The Assessment And Application Of Technology | Method for producing polyploids of para rubber tree |
| US20160219801A1 (en) * | 2015-02-03 | 2016-08-04 | Sumitomo Rubber Industries, Ltd. | Method of regenerating rubber tree, method of propagating rubber tree, method of inducing shoot, method of elongating shoot, method of rooting shoot, and method of acclimatizing young plant |
| CN115184546A (en) * | 2022-06-21 | 2022-10-14 | 云南省热带作物科学研究所 | Method for rapidly identifying and selecting rubber tree triploid plants in field |
| CN115281076A (en) * | 2022-08-04 | 2022-11-04 | 北京林业大学 | Plant polyploid induction device |
-
2023
- 2023-02-04 CN CN202310055200.6A patent/CN116267594B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1669408A (en) * | 2005-04-20 | 2005-09-21 | 北京林业大学 | High temperature duplicater for trees |
| WO2010034961A1 (en) * | 2008-09-23 | 2010-04-01 | Sumatra Biosciences Pte Ltd | Methods of obtaining hevea bras iliensis plants |
| US20140223813A1 (en) * | 2011-06-10 | 2014-08-14 | The Agency For The Assessment And Application Of Technology | Method for producing polyploids of para rubber tree |
| US20160219801A1 (en) * | 2015-02-03 | 2016-08-04 | Sumitomo Rubber Industries, Ltd. | Method of regenerating rubber tree, method of propagating rubber tree, method of inducing shoot, method of elongating shoot, method of rooting shoot, and method of acclimatizing young plant |
| CN115184546A (en) * | 2022-06-21 | 2022-10-14 | 云南省热带作物科学研究所 | Method for rapidly identifying and selecting rubber tree triploid plants in field |
| CN115281076A (en) * | 2022-08-04 | 2022-11-04 | 北京林业大学 | Plant polyploid induction device |
Non-Patent Citations (3)
| Title |
|---|
| 吴裕等: "云南山地橡胶树三倍体育种回顾", 热带农业科技, vol. 43, no. 3, pages 37 - 40 * |
| 张源源等: "橡胶树三倍体的2n配子来源及发生途径鉴定", 南方农业学报, vol. 49, no. 02, pages 208 - 213 * |
| 张源源等: "温度对橡胶树GT1的2n雌配子发生频率的影响", 西南农业学报, vol. 30, no. 12, pages 2634 - 2638 * |
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