CN116254173B

CN116254173B - Sampling device and sampling method suitable for animal and plant cells

Info

Publication number: CN116254173B
Application number: CN202310548700.3A
Authority: CN
Inventors: 王晓武; 袁河链; 武剑; 陈思耿; 吴永锦; 吴晓俊
Original assignee: Guangdong Qijiang Technology Co ltd; Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences
Current assignee: Guangdong Qijiang Technology Co ltd; Institute of Vegetables and Flowers Chinese Academy of Agricultural Sciences
Priority date: 2023-05-16
Filing date: 2023-05-16
Publication date: 2023-08-01
Anticipated expiration: 2043-05-16
Also published as: CN116254173A

Abstract

The invention provides a sampling device and a sampling method suitable for animal and plant cells, wherein the sampling device comprises a shell which forms the external shape of the sampling device, a worktable which is convenient to operate, a registration management unit and a sampling unit which are fixed on the worktable, a sample storage unit which is detachably and movably arranged below the worktable and is arranged corresponding to the position of the sampling unit, and a driving unit which is used for driving the sample storage unit to move along the X, Y axis.

Description

Sampling device and sampling method suitable for animal and plant cells

Technical Field

The invention relates to the field of biological detection, in particular to a sampling device and a sampling method suitable for animal and plant cells.

Background

With the increasing development of biological detection technology, great improvement of animal and plant DNA extraction and purification efficiency, namely high-flux DNA extraction and purification technology, is urgently needed. The magnetic bead extraction and purification method of DNA and the corresponding extraction equipment, namely a high-flux DNA extraction and purification instrument, are the most basic method and equipment at present. For plant DNA extraction, plant leaves and samples are required to be placed in a standard pore plate, and the operation is still carried out in a manual mode at present, so that the operation is tedious and repeated, the labor intensity is high, the sample collection errors are easy to cause, and the production and scientific research efficiency and the working quality are greatly influenced.

In the prior art, CN114858505B provides a plant leaf sampling instrument, can hold and carry out plant leaf sampling, convenient to use can greatly improve test personnel's work efficiency, but in actual use, the actual conditions is that laboratory personnel gather a large amount of plant specimens and carry out filing classification in the laboratory, plant specimens just need classify, register and save after pressing, bind, the sampling process needs at first to classification, register, save just pasting the relevant information of plant specimens on the platen paper, then according to certain rule, carry out classification collection sample, so that research uses, prior art's sampling process needs the laboratory personnel to record one by one, when sample quantity is very big, sample record or seek certain plant specimens just appear more troublesome in the plant room, and appear very easily to record wrong condition, especially for biological novice.

To solve this problem, the plant leaf sampling apparatus of the prior art can only perform automatic sampling, and cannot perform synchronous recording on the sampled samples.

It should be noted that the information in the background is only for the purpose of illustrating the general background of the invention and should not be taken as an admission or any form of suggestion that such information forms the prior art that is well known to those of ordinary skill in the art.

Disclosure of Invention

In some embodiments of the present application, a sampling device and a sampling method suitable for animal and plant cells are provided, which solve the problem of how to perform a large number of animal and plant cell sampling processes in a laboratory, and how to perform synchronous management and registration of collected samples during sample sampling process while improving sampling efficiency, avoid trouble of searching after sampling, and reduce registration errors.

In some embodiments of the application, the sampling device is improved, the registration management unit is added on the full-automatic sampling device, the sampling device comprises the registration management unit and the sampling unit which are fixed on the workbench surface, the sample storage unit which is detachably and movably installed below the workbench surface and is correspondingly arranged with the position of the sampling unit, and the driving unit which is used for driving the sample storage unit to move along the X, Y axis.

In some embodiments of the application, a sampling device is disclosed, it includes the casing, table surface, register management unit, sampling unit, sample is deposited unit and drive unit, register management unit is fixed on table surface, register management unit is used for registering the sample, sampling unit installs on table surface for cut out the sample of required size from the sample, and the through-hole has been seted up with sampling unit corresponding position on the table surface, through-hole can allow sampling unit at least part to get into, sample is deposited the unit and is installed under table surface, sample is deposited the unit detachable and install in drive unit, drive unit is used for driving the sample and deposits the unit and move in X direction and Y direction.

The sampling unit is used for placing the sample with the required size into the sample storage unit through the through hole.

In some embodiments of the present application, the registration management unit includes barcode scanning gun, scanning gun head cover, camera and photographic lamp, and barcode scanning gun is used for discernment barcode, and scanning gun head cover cladding is in the barcode scanning gun outside, and the camera setting is in scanning gun head cover for shoot the record to the sample, photographic lamp sets up in one side of camera for carry out the light filling to the shooting process of camera.

In some embodiments of the application, the sampling unit includes sampling drift, punch head cover and gear motor, sampling drift corresponds with the through-hole position, and sampling drift's end portion can hold in the through-hole, sampling drift moves along the Z axle direction, the outside at sampling drift is covered to the punch head cover, the inside spacing groove that is equipped with of punch head cover, sampling drift installs in the spacing inslot, the spacing groove is used for carrying out up-and-down motion with sampling drift in the Z axle direction, gear motor's motor shaft is fixed with crank link mechanism, crank link mechanism is used for converting gear motor's rotation motion into sampling drift along the up-and-down motion of Z axle direction.

In some embodiments of the application, sampling punch is inside cavity and wears to be equipped with the movable rod, and movable rod one end is fixed with electromagnetic drive device, and electromagnetic drive device is used for controlling the movable rod and moves along the extending direction of sampling punch, and the movable rod other end is provided with hydrophobic brush hair, and the movable rod activity drives hydrophobic brush hair and stretches out or retract by the end part of sampling punch, and the sample of attaching at the end part of sampling punch can fall into in the sample storage unit under the promotion effect of hydrophobic brush hair.

In some embodiments of the present application, the sample storage unit is arrayed with a plurality of holding bins for holding samples, the holding bins correspond to the through holes, the driving unit drives the preset holding bins to move to the positions corresponding to the through holes, and the sampling unit places the samples with the required cutting sizes in the preset holding bins through the through holes.

In some embodiments of the application, the drive unit includes placing portion, X axle drive assembly and Y axle drive assembly, placing portion is used for fixed sample to deposit the unit, X axle drive assembly includes first step motor and first removal slide rail, first removal slide rail is arranged along the X axle direction, placing portion installs on first removal slide rail, first step motor is used for driving placing portion and moves along first removal slide rail activity, Y axle drive assembly includes second step motor and second removal slide rail, the second removes the slide rail and arranges along the Y axle direction, X axle drive assembly installs on the second removes the slide rail, the second step motor is used for driving X axle drive assembly and moves along the second removal slide rail activity.

In some embodiments of the present application, the sampling device further includes a central control unit, the central control unit is electrically connected to the registration management unit, and is used for storing and archiving data information of the sampled specimen registered by the registration management unit, the central control unit is further electrically connected with the sampling unit and the driving unit, and is used for controlling the sampling unit to sample, and controlling the driving unit to drive the sample storing unit to move.

In some embodiments of the present application, a sampling method suitable for animal and plant cells is further included, and is suitable for the above sampling device, wherein the sample storage unit is provided with a plurality of storage bins for storing samples, the storage bins are used for storing samples, before sampling, a sample bar code is generated by a computer, and is printed out by a bar code machine, the sample bar code is pasted on a sample, and the sample bar code is associated with information of the sample, and the sampling method comprises:

s1: the sample storage unit is registered, the registration management unit scans the storage bar codes, the storage unit information generated by scanning is registered and stored in the central control unit, the sample storage unit is installed in place on the driving unit after registration, and the driving unit drives the sample storage unit to move to any accommodating bin to be aligned with the through hole;

s2: the method comprises the steps of registering a sample, scanning the sample with a sample bar code in a registration management unit, photographing the sample, registering scanning information generated by scanning the sample bar code and image information generated by photographing the sample in a central control unit, and associating the scanning information with the image information;

s3: sampling a sample, placing the registered sample on a workbench surface, enabling the placing position to correspond to the through hole, starting a sampling unit, cutting the sample with a required size from the sample by the sampling unit, pushing the acquired sample into a containing bin through the through hole, recording the position information of the containing bin where the current sample is placed on a sample storage unit by a central control unit, and mapping the position information with the sample scanning information and the sample image information registered in the step S2;

the central control unit records the accommodating bin with the sample as full load and records the accommodating bin without the sample as empty load;

s4: after the accommodating bin in the S3 process is fully loaded, the driving unit is started to move any empty accommodating bin to the corresponding position of the through hole, and the S2-S3 process is repeated;

s5: and replacing the sample storage unit, taking down the current sample storage unit after all the accommodating bins of the sample storage unit are recorded to be full, replacing another sample storage unit, and repeating the processes S1-S4.

In some embodiments of the present application, the sample storage unit is a digital signal having 12 columns in the longitudinal direction and 8 rows in the transverse direction, the digital signal having 96 storage bins, and the position information is the arrangement coordinates of the storage bins on the orifice plate.

The invention has the beneficial effects that:

the adoption of the registration management unit is beneficial to conveniently and rapidly registering related samples, and the sample registration is carried out in the sample sampling process, so that the subsequent sample searching and reading of the information of the related plant samples are facilitated.

The sampling process is full-automatic, a large number of plant samples can be rapidly and efficiently collected, and the method is particularly suitable for the field of high-flux plant DNA extraction with high labor intensity and complex and repeated work.

Drawings

FIG. 1 is a schematic diagram of the whole structure of a sampling device according to the present invention;

FIG. 2 is an enlarged view of the invention at A of FIG. 1;

FIG. 3 is a schematic diagram of the whole structure of the sampling device of the present invention;

FIG. 4 is a schematic diagram of the internal structure of the sampling device of the present invention;

FIG. 5 is a schematic diagram showing an exploded structure of the registration management unit of the present invention;

FIG. 6 is a schematic diagram of the external structure of a sampling unit according to the present invention;

FIG. 7 is a schematic diagram of an exploded construction of the sampling unit of the present invention;

FIG. 8 is a schematic view of the mounting structure of the sample storage unit and the drive unit of the present invention;

FIG. 9 is a schematic view showing an exploded structure of the sample storage unit and the drive unit of the present invention;

FIG. 10 is a schematic view of the connection structure of the electrical components of the present invention;

FIG. 11 is a representation of the bin coordinates of the sample storage unit of the present invention.

Reference numerals:

comprising the following steps: 100. a sampling device; 110. a housing; 120. a work table; 121. a through hole; 200. a registration management unit; 210. a bar code scanning gun; 220. scanning a gun head cover; 230. a camera; 240. a photographic lamp; 300. a sampling unit; 310. sampling a punch; 320. a punch head cover; 330. a speed reducing motor; 340. a crank-link mechanism; 350. a movable rod; 360. an electromagnetic driving device; 370. hydrophobic bristles; 400. a sample storage unit; 410. a receiving bin; 500. a driving unit; 510. a placement unit; 520. an X-axis driving assembly; 530. a Y-axis drive assembly; 521. a first stepping motor; 522. a first movable slide rail; 531. a second stepping motor; 532. the second movable slide rail.

Detailed Description

The following describes in further detail the embodiments of the present invention with reference to the drawings and examples. The following examples are illustrative of the invention and are not intended to limit the scope of the invention.

In some embodiments of the present application, as shown in fig. 1-10, a sampling device 100 is disclosed that includes a housing 110, a countertop 120, a registration management unit 200, a sampling unit 300, a sample storage unit 400, and a drive unit 500.

The registration management unit 200 is fixed to the table 120, and the registration management unit 200 is used for registering the sampled specimen.

The sampling unit 300 is mounted on the workbench 120, and is used for cutting a sample with a required size from a specimen, and a through hole 121 is formed in the workbench 120 at a position corresponding to the sampling unit 300, and the through hole 121 can allow at least a part of more end parts of the sampling unit 300 to enter.

The sample storage unit 400 is mounted under the workbench 120, the sample storage unit 400 is detachably mounted on the driving unit 500, and the driving unit 500 is used for driving the sample storage unit 400 to move in the X direction and the Y direction.

Wherein the sampling unit 300 places a sample of a cut desired size into the sample storage unit 400 through the through-hole 121.

It should be noted that, the sampling process is fully automatic, can collect a large amount of plant samples fast and efficiently, is particularly suitable for the field of high-throughput plant DNA extraction with high labor intensity and complex and repeated work, and the adoption of the registration management unit 200 is beneficial to conveniently and fast registering related samples, and the sample registration is performed in the sample sampling process, so that the subsequent sample searching and reading of the information of related plant samples are convenient.

Based on the above-described embodiment, as shown in fig. 1, 3, 4, and 5, the registration management unit 200 includes a barcode scanning gun 210, a scanning gun head 220, a camera 230, and a photographic lamp 240.

The barcode scanning gun 210 is used for recognizing a barcode, the scanning gun head cover 220 is coated on the outer side of the barcode scanning gun 210, the camera 230 is arranged on the scanning gun head cover 220 and used for shooting and recording a specimen, and the photographic lamp 240 is arranged on one side of the camera 230 and used for supplementing light in the shooting process of the camera 230.

The specimen bar code is generated by a computer, printed by a bar code machine, stuck on the specimen and correlated with the information of the specimen.

The use of the specimen barcode and barcode scanning gun 210 is beneficial for conveniently and quickly reading information about the relevant plant specimen and entering into a computer.

Based on the above embodiment, the sample registration process is to turn on the computer, turn on the photographing lamp 240 and the camera 230, photograph the sample, and simultaneously record the sample information bar code into the computer through the bar code scanning gun 210 and correlate the sample information bar code with the photograph (sample image information) of the sample and the sample bar code serial number (sample scanning information), to perform the sampling, and the sampling process maps the position information (position information) of the sample stored in the sample storage unit 400 with the sample scanning information and the sample image information registered therein.

In some embodiments of the present application, as shown in fig. 1-4 and 6-7, the sampling unit 300 includes a sampling punch 310, a punch cover 320, and a gear motor 330;

the sampling punch 310 corresponds in position to the through hole 121, and an end portion of the sampling punch 310 is receivable in the through hole 121, and the sampling punch 310 moves in the Z-axis direction.

The punch housing 320 is coated on the outer portion of the sampling punch 310, a limiting groove is formed in the punch housing 320, the sampling punch 310 is installed in the limiting groove, and the limiting groove is used for limiting the sampling punch 310 to move up and down in the Z-axis direction.

A crank link mechanism 340 is fixed to a motor shaft of the speed reduction motor 330.

The rotational force of the speed reducing motor 330 is converted into a linear motion of the sampling punch 310, preferably a vertical up-and-down motion, i.e., an up-and-down motion of the sampling punch 310 in the Z-axis direction, by a crank-link structure.

The end of the sampling punch 310, i.e., the portion for cutting out the plant specimen, has a structure that facilitates detachment of the plant specimen from the plant body.

The sampling punch 310 can have different diameters and cross-sectional shapes, whereby the sample size and sample shape that can be cut can be selected as desired.

The sampling punches 310 can be fixedly attached or removably attached to the crank mechanism 340 at the upper portion of the sampling punches 310, and in some exemplary embodiments, each sampling punch 310 can be removably mounted to the crank mechanism 340.

Based on this, each sampling punch 310 has a particular different model, which can be numbered by the sample size that the sampling head can cut, or by the shape of the sample that is cut, for example, the shape of the sampling punch 310 includes, but is not limited to, any shape of raised structures, such as a straight, cross, rice, conical, inverted cone raised structures.

In further exemplary embodiments, the number of sampling punches 310 is not particularly limited, and may be one or more, and a plurality of sampling punches 310 may be linearly arranged or otherwise arranged on the crank mechanism 340.

In addition, as will be further understood by those skilled in the art, the sampling punch 310 is mounted in a limiting slot, and the limiting slot is mainly used for limiting the sampling punch 310 to move up and down in the Z-axis direction, in some cases, the limiting slot may not be provided at all, and the up and down movement of the sampling punch 310 is not affected, or the limiting slot may be replaced by another component capable of limiting the up and down movement of the sampling punch 310.

In some embodiments of the present application, as shown in fig. 7, the sampling punch 310 is hollow inside and is perforated with a movable rod 350.

An electromagnetic driving device 360 is fixed at one end of the movable rod 350, and the electromagnetic driving device 360 is used for controlling the movable rod 350 to move along the extending direction of the sampling punch 310.

The other end of the movable bar 350 is provided with hydrophobic bristles 370.

The movable rod 350 moves to drive the hydrophobic brush 370 to extend or retract from the end portion of the sampling punch 310, and the sample attached to the end portion of the sampling punch 310 can fall into the sample storage unit 400 under the pushing action of the hydrophobic brush 370.

The hydrophobic brush 370 can detach the sample from the sampling punch 310 and enter the sample storage unit 400 when the sample storage unit 400 is used, so as to reduce residues of the sample possibly existing at the end of the sampling punch 310.

In the present invention, the electromagnetic driving means 360 includes an electromagnet and a permanent magnet fixed to one end of the movable bar 350. It will be appreciated that the electromagnet and the permanent magnet may be spaced apart by a certain distance, preferably, when the electromagnet is energized, the electromagnet attracts the permanent magnet with different poles to form a tight connection, the hydrophobic bristles 370 are located inside the end portion of the sampling punch 310, and the sampling punch 310 is driven by the speed reducing motor 330 to move along the Z-axis to sample the specimen.

At this time, the electromagnet is reversely electrified, the magnetic poles are switched, the homopolar of the electromagnet and the homopolar of the permanent magnet block are repulsed and separated, the end part of the hydrophobic brush hair 370, which is provided with the sampling punch 310, extends out, and part of the sample attached to the sampling punch 310 is pushed into the sample storage unit 400.

In an exemplary embodiment, the movable bar 350 is configured to be rotatable at least as the sampling punch 310 moves downward.

In some embodiments of the present application, as shown in fig. 4, 8, 9 and 11, a plurality of holding chambers 410 for holding a sample are arrayed on the sample storage unit 400.

The receiving bin 410 corresponds to the position of the through hole 121, and the driving unit 500 drives the preset receiving bin 410 to move to the position corresponding to the through hole 121, and the sampling unit 300 places the sample of the required size cut out into the preset receiving bin 410 through the through hole 121.

It should be noted that, the sample storage unit 400 can be driven by the driving unit 500 to move in the X direction and the Y direction, so that each of the plurality of holding bins 410 can sequentially correspond to the through hole 121, and the required plant sample is cut into each holding bin 410 by moving the sampling punch 310 downward.

It should be further noted that, the sample storage unit 400 is also preferably configured according to the flux in other instruments, such as a high-flux DNA or RNA extractor, which are matched with the sample storage unit 400, and the sample storage unit 400 has a structure matched with the other instruments, such as the high-flux DNA extractor, so that the collected sample can be used in a subsequent process after being collected. For example, the sample storage unit 400 may be designed to have a general structure with a blade-implanted well plate instrument such that the sample storage unit 400 removed from the instrument of the present invention is directly mounted to the blade-implanted well plate instrument, and automatically loads a plant sample into a corresponding well site in the well plate.

It is further noted that in the present invention, the containment bin 410 is configured to be capable of holding at least a portion of a plant, preferably a plant portion containing a desired sample of tissue. For example, leaves, rhizomes, etc., preferably sheet-like samples, in particular leaves.

The bottom of the housing 410 may be flat or may be configured to have an arcuate configuration suitable for securing the desired plant parts.

The shape of the receiving bin 410 is not particularly limited and may be freely set as required, and the receiving bin 410 may be circular, square, star-shaped, triangular or any other shape, and preferably the receiving bin 410 and the through hole 121 are identical in cross-sectional shape to the sampling punch 310. For example, when the through hole 121 is circular in shape, the receiving compartment 410 is also circular in shape. When the through-hole 121 is square in shape, the receiving bin 410 is also square in shape.

The drive unit 500 moves the sample storage unit 400 to any one of the receptacles 410 in alignment with the through-hole 121, and the number of receptacles 410 in the present storage unit includes, but is not limited to, 6, 12, 24, 48, 96, 128, 384, for example, in the exemplary embodiment, the sample storage unit 400 has 12 columns longitudinally and 8 rows transversely of well plates having 96 receptacles 410.

In some embodiments of the present application, as shown in fig. 1, 3, 4, 8, and 9, the drive unit 500 includes a placement portion 510, an X-axis drive assembly 520, and a Y-axis drive assembly 530.

The placement portion 510 is used to fix the sample storage unit 400.

The X-axis driving assembly 520 includes a first stepping motor 521 and a first moving rail 522, the first moving rail 522 is disposed along the X-axis direction, the placement portion 510 is mounted on the first moving rail 522, and the first stepping motor 521 is used for driving the placement portion 510 to move along the first moving rail 522.

The Y-axis driving assembly 530 includes a second stepping motor 531 and a second moving rail 532, the second moving rail 532 is disposed along the Y-axis direction, the X-axis driving assembly 520 is mounted on the second moving rail 532, and the second stepping motor 531 is used for driving the X-axis driving assembly 520 to move along the second moving rail 532.

It should be noted that, the X-axis driving assembly 520 and the Y-axis driving assembly 530 move the sample storage unit 400 at a fixed distance, so that each of the receiving chambers 410 sequentially corresponds to the through hole 121.

In order to allow the first stepping motor 521 and the second stepping motor 531 to cooperatively operate in a predetermined manner, it is preferable that control be performed by a control circuit.

The control circuit operates in a defined manner by controlling, for example, the successive or regular activation of the individual motors.

The first stepping motor 521 and the second stepping motor 531 of the present invention have a structure suitable for miniaturization and compact arrangement.

In the present invention, the driving methods of the first and second moving rails 522 and 532 and the first and second stepping motors 521 and 531 for guiding the movement of the sample storage unit 400 are known in the art, and are not particularly limited thereto.

In some embodiments of the present application, as shown in fig. 10, the sampling device 100 further includes a central control unit.

The central control unit comprises the computer.

The central control unit is electrically connected to the registration management unit 200 and is used for storing and archiving data information of the sampling specimen registered by the registration management unit 200.

The central control unit is further electrically connected with the sampling unit 300 and the driving unit 500, and is used for controlling the sampling unit 300 to sample and controlling the driving unit 500 to drive the sample storage unit 400 to move.

Based on the above embodiment, a sampling method suitable for animal and plant cells is also included, which is suitable for the sampling device 100 shown in fig. 1 to 10.

Before sampling, a computer is used for generating a specimen bar code, the specimen bar code is printed out through a bar code machine, the specimen bar code is stuck on a specimen, and the specimen bar code is associated with information of the specimen.

The sampling method comprises the following steps:

s1: the sample storage unit 400 is registered, the registration management unit 200 scans the storage bar codes, the storage unit information generated by scanning is registered and stored in the central control unit, the sample storage unit 400 is installed in place on the driving unit 500 after registration, and the driving unit 500 drives the sample storage unit 400 to move to any accommodating bin 410 to be aligned with the through hole 121;

s2: the sample registration, the sample with the sample bar code is scanned in the registration management unit 200, the sample is shot, the scanning information generated by scanning the sample bar code and the image information generated by shooting the sample are registered in the central control unit, and the scanning information and the image information are associated;

s3: sampling a sample, placing the registered sample on the workbench surface 120, wherein the placement position corresponds to the through hole 121, starting the sampling unit 300, cutting the sample with a required size from the sample by the sampling unit 300, pushing the acquired sample into the accommodating bin 410 through the through hole 121, recording the position information of the accommodating bin 410 where the current sample is placed on the sample storage unit 400 by the central control unit, and mapping the position information with the sample scanning information and the sample image information registered in the S2;

wherein, the central control unit records the accommodating bin 410 with the sample as full load and records the accommodating bin 410 without the sample as empty load;

s4: after the accommodating bin 410 in the S3 process is fully loaded, the driving unit 500 is started to move any empty accommodating bin 410 to the corresponding position of the through hole 121, and the S2-S3 process is repeated;

s5: the sample storage unit 400 is replaced, and after all the storage bins 410 of the sample storage unit 400 are recorded as full, the current sample storage unit 400 is removed, another sample storage unit 400 is replaced, and the processes S1 to S4 are repeated.

In some embodiments of the present application, the sample storage unit 400 has 12 columns in the longitudinal direction and 8 rows in the transverse direction of an orifice plate having 96 storage bins 410, and the position information is a digital signal of the arrangement coordinates of the storage bins 410 on the orifice plate.

Based on the basic technical concept, in actual use of the sampling device 100, taking a leaf sampling of poplar as an example, the working process is as follows:

firstly, before sampling, a sample bar code is generated by a computer and printed out by a bar code machine, the sample bar code is stuck on a blade, and the blade is numbered as blade a.

The sample storage unit 400 is taken out, a storage bar code is generated by a computer and printed out by a bar code machine, the storage bar code is stuck on the sample storage unit 400, the sample storage unit 400 is a number A, wherein the sample storage unit 400 is selected from 96 holes with 12 columns in the longitudinal direction and 8 rows with 96 containing bins 410 in the transverse direction.

The scanning gun scans the stored bar codes, the generated storage unit information after scanning is registered and stored in the computer of the central control unit, the computer records that the current sample storage unit 400 is a 96-well plate with the number A, meanwhile, the computer carries out coordinate marking on the position of each containing bin 410 of the 96-well plate, the horizontal and vertical coordinates are respectively in the row and column arrangement sequence, as shown in fig. 11, the containing bin 410 at the lower left corner is (1, 1) and is the first row of the first row, the left lower corner begins with the containing bin 410 coordinate at the lower left corner, the right horizontal coordinate is increased, and the upward vertical coordinate is increased.

The positions of the pockets 410 are shown as (1, 1) and (7, 5) in fig. 11.

After registration, the 96-well plate with the number A is installed in place on the driving unit 500, and is adjusted by the first stepping motor 521 and the second stepping motor 531 to drive the 96-well plate to move along the first moving slide rail 522 and the second moving slide rail 532, so that the accommodating bin 410 with the coordinates (1, 1) is aligned with the through hole 121.

Taking out a blade sample, scanning a sample bar code of the blade a on a scanning gun, registering the current sample to be sampled as the blade a by a computer, storing scanning information, photographing the blade a by using a camera 230, forming image information of the blade a, and associating the image information with the scanning information.

The scanned and photographed blade a is placed on the table 120, and the sampling punch 310 is started to cut a part of the blade a into a blade sample with a diameter of about 4 mm.

The sampling punch 310 continues to move down along the Z-axis, pushing the collected sample through the through-hole 121 into the receiving pocket 410 corresponding to the through-hole 121, the current receiving pocket 410 being (1, 1) in coordinates.

The electromagnetic drive 360 is activated and the hydrophobic bristles 370 extend from the interior of the sampling punch 310, pushing some of the sample fragments remaining attached to the end portion of the sampling punch 310 into the containment bin 410 (1, 1), cleaning the end portion of the sampling punch 310, and thereafter, retracting the hydrophobic bristles 370 into the sampling punch 310.

The computer records that the sample of blade a is placed in the 96-well plate (1, 1) containing bin 410 numbered a and maps the position information with registered sample scan information and sample image information.

The computer marks the bin 410 with coordinates (1, 1) as full.

The driving unit 500 is activated to control the 96-well plate to move by the movement of the first and second stepping motors 521 and 531, aligning the accommodating chambers 410 having coordinates (1, 2) with the through holes 121.

Blade b is removed and the above operation is repeated to perform sample collection of blade b.

The first concept of the invention improves the sampling device 100, adds a registration management unit 200 on the full-automatic sampling device 100, and the sampling device 100 comprises a registration management unit 200 and a sampling unit 300 which are fixed on a working table 120, a sample storage unit 400 which is detachably and movably arranged below the working table 120 and is arranged corresponding to the position of the sampling unit 300, and a driving unit 500 for driving the sample storage unit 400 to move along the X, Y axis.

The invention has the beneficial effects that:

(1) The adoption of the registration management unit is beneficial to conveniently and rapidly registering related samples, and the sample registration is carried out in the sample sampling process, so that the subsequent sample searching and reading of the information of the related plant samples are facilitated.

(2) The sampling process is full-automatic, a large number of plant samples can be rapidly and efficiently collected, and the method is particularly suitable for the field of high-flux plant DNA extraction with high labor intensity and complex and repeated work.

Those of ordinary skill in the art will appreciate that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. The laboratory sampling device suitable for the animal and plant cells is characterized by comprising a shell, a workbench surface, a registration management unit, a sampling unit, a sample storage unit, a driving unit and a central control unit; wherein:

the registration management unit is fixed on the workbench surface, is arranged to be capable of scanning the storage bar codes, registers and stores storage unit information generated by scanning in the central control unit, installs the sample storage unit in place on the driving unit after registration, and drives the sample storage unit to move to any accommodating bin to be aligned with the through hole;

the sampling unit is arranged on the workbench surface and is used for cutting samples with required sizes from the samples, through holes are formed in positions, corresponding to the sampling unit, on the workbench surface, and the through holes can allow at least one part of the sampling unit to enter, so that the samples with the required sizes are cut and placed in the sample storage unit through the through holes;

the sample storage unit is arranged below the workbench surface, a plurality of accommodating bins for accommodating samples are arrayed on the sample storage unit, and the sample storage unit is detachably arranged on the driving unit;

the driving unit is used for driving the sample storage unit to move in the X direction and the Y direction, so that any accommodating bin can be driven by the driving unit to move to the position corresponding to the through hole, the driving unit comprises an X-axis driving assembly, a Y-axis driving assembly and a placement part, the X-axis driving assembly comprises a first stepping motor and a first moving slide rail, the first moving slide rail is arranged along the X axis direction, the placement part is mounted on the first moving slide rail, the first stepping motor is used for driving the placement part to move along the first moving slide rail, the Y-axis driving assembly comprises a second stepping motor and a second moving slide rail, the second moving slide rail is arranged along the Y axis direction, the X-axis driving assembly is mounted on the second moving slide rail, the second stepping motor is used for driving the X-axis driving assembly to move along the second moving slide rail, and the placement part is used for fixing the sample storage unit;

the central control unit is electrically connected with the registration management unit and is used for storing and archiving data information of the sampled specimens registered by the registration management unit, and the central control unit is also electrically connected with the sampling unit and the driving unit and is used for controlling the sampling unit to sample and controlling the driving unit to drive the sample storage unit to move; the central control unit is further configured to: after the collected samples are pushed into the accommodating bin through the through holes, the central control unit records the position information of the accommodating bin where the current samples are placed on the sample storage unit, maps the position information with registered sample scanning information, and records the accommodating bin where the samples are placed as full load.

2. The laboratory sampling device according to claim 1, wherein the enrollment management unit comprises:

the bar code scanning gun is used for identifying bar codes;

the scanning gun head cover is coated on the outer side of the bar code scanning gun;

the camera is arranged in the scanning gun head cover and is used for shooting and recording the specimen;

and the photographic lamp is arranged at one side of the camera.

3. The laboratory sampling device according to claim 1, wherein the sampling unit comprises:

the sampling punch head corresponds to the through hole in position, the end head part of the sampling punch head can be accommodated in the through hole, and the sampling punch head moves along the Z-axis direction;

the punch head cover is coated on the outer part of the sampling punch head, a limiting groove is formed in the punch head cover, the sampling punch head is arranged in the limiting groove, and the limiting groove is used for limiting the sampling punch head to move in the Z-axis direction;

and the motor shaft of the speed reducing motor is fixed with a crank connecting rod mechanism, and the crank connecting rod mechanism is used for converting the rotation motion of the speed reducing motor into the motion of the sampling punch along the Z-axis direction.

4. A laboratory sampling device according to claim 3, wherein the sampling punch is hollow and is perforated with a movable rod;

an electromagnetic driving device is fixed at one end of the movable rod and used for controlling the movable rod to move along the extending direction of the sampling punch;

the other end of the movable rod is provided with hydrophobic bristles;

the movable rod is used for movably driving the hydrophobic brush hair to extend out or retract back from the end head part of the sampling punch, and a sample attached to the end head part of the sampling punch can fall into the sample storage unit under the pushing action of the hydrophobic brush hair.

5. A laboratory sampling method for animal and plant cells, characterized in that it is applied to the laboratory sampling device according to any one of claims 1 to 4;

before sampling, a storage bar code and a specimen bar code are generated by a computer, and are printed out by a bar code machine, the storage bar code is stuck on the specimen storage unit, the specimen bar code is stuck on a specimen, and the specimen bar code is associated with information of the specimen;

the sampling method comprises the following steps:

s1: the sample storage unit is registered, the registration management unit scans the storage bar code, the storage unit information generated by scanning is registered and stored in the central control unit, the sample storage unit is installed in place on the driving unit after registration, and the driving unit drives the sample storage unit to move to any one of the accommodating bins to be aligned with the through hole;

s2: a sample registration, wherein a sample with a sample bar code is scanned in the registration management unit, the sample is shot, scanning information generated by scanning the sample bar code and image information generated by shooting the sample are registered in the central control unit, and the scanning information and the image information are associated;

s3: sample sampling, namely placing a registered sample on the workbench surface, wherein the placement position corresponds to the through hole, starting the sampling unit, cutting a sample with a required size from the sample by the sampling unit, pushing the acquired sample into the accommodating bin through the through hole, recording the position information of the accommodating bin, where the current sample is placed, on the sample storage unit by the central control unit, and mapping the position information with the sample scanning information and the sample image information registered in the step S2;

6. The sampling method according to claim 5, wherein the sample storage unit has 12 columns in a longitudinal direction and 8 rows in a transverse direction of an orifice plate having 96 of the holding chambers, and the positional information is a digital signal of an arrangement coordinate of the holding chambers on the orifice plate.