CN116218797A - Method for rapidly preparing soil complex enzyme - Google Patents
Method for rapidly preparing soil complex enzyme Download PDFInfo
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- CN116218797A CN116218797A CN202310433348.9A CN202310433348A CN116218797A CN 116218797 A CN116218797 A CN 116218797A CN 202310433348 A CN202310433348 A CN 202310433348A CN 116218797 A CN116218797 A CN 116218797A
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- soil
- complex enzyme
- preparing
- liquid carbon
- microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
A method for rapidly preparing soil complex enzyme comprises the steps of firstly, preparing fertilizer; step two, preparing soil; step three, mixing and stirring; step four, strain introduction; step five, natural fermentation; step six, microorganism propagation; step seven, preparing soil complex enzyme; in the seventh step, a microbial cell wall breaking machine is used for breaking the wall of the soil after the propagation of the microorganisms to release active spores; the invention adopts the liquid carbon-based fertilizer and the actinomycetes to prepare the soil compound enzyme, which can accelerate the metabolism of microorganisms in the soil, activate mineral elements in the soil, avoid the hardening of the soil, generate endogenous hormones, resistant substances and a large amount of organic micromolecules, lighten the unbalance of the proportion of the microorganisms in the soil to a certain extent, effectively inhibit the propagation of fungi in the soil, increase the quantity of beneficial actinomycetes and maintain the balance of the microorganisms in the soil, thereby achieving the aim of ensuring the high yield, the high quality and the health of the soil of crops.
Description
Technical Field
The invention relates to the technical field of complex enzyme preparation, in particular to a method for rapidly preparing soil complex enzyme.
Background
After the development stage of the original agriculture, the agricultural production is changed into a modern agriculture stage, the modern agriculture has the production characteristics of sufficient irrigation water, a large amount of pesticides and fertilizers, and the yield is improved, meanwhile, the following negative effects are brought to the improvement of the yield, firstly, the soil environment is worsened, mineral elements are accumulated to cause soil hardening and secondary salinization, secondly, the proportion of microorganisms in the soil is seriously unbalanced, the quality of agricultural products is reduced, thirdly, fungi are greatly propagated, and the soil productivity is seriously reduced due to the lack of beneficial actinomycetes.
Disclosure of Invention
The invention aims to provide a method for rapidly preparing soil complex enzyme, which aims to solve the problems in the background technology.
In order to achieve the above purpose, the present invention provides the following technical solutions: a method for rapidly preparing soil complex enzyme comprises the steps of firstly, preparing fertilizer; step two, preparing soil; step three, mixing and stirring; step four, strain introduction; step five, natural fermentation; step six, microorganism propagation; step seven, preparing soil complex enzyme;
wherein in the first step, a liquid carbon-based fertilizer is prepared;
in the second step, no-tillage soil for no tillage or loose and fertile soil with uniform soil structure is selected;
in the third step, the liquid carbon-based fertilizer prepared in the first step and the soil in the second step are mixed and stirred uniformly;
in the fourth step, dry yeast is added into the soil obtained in the third step for mixing and stirring;
in the fifth step, a proper position is selected, and the mixture in the fourth step is naturally fermented;
in the sixth step, the microorganisms in the fifth step are propagated;
in the seventh step, the microorganism in the sixth step is broken to prepare the soil complex enzyme.
Preferably, in the first step, the production of the liquid carbon-based fertilizer includes the following steps: collecting natural organic matters, sorting, removing impurities, pulverizing, degrading, activating, decompressing, blasting, concentrating, and preparing into the final product, wherein the natural organic matters comprise crop straw, dry livestock manure or weathered coal.
Preferably, in the first step, 72 medium trace elements in the liquid carbon-based fertilizer are more than or equal to 20g/L, and active organic matters (fulvic acid) are more than or equal to 120g/L, and N+P 2 O 5 +K 2 O≥200g/L。
Preferably, in the second step, after selecting a suitable soil, a screening machine is used to simply remove impurities such as stones and remaining garbage possibly existing in the soil.
Preferably, in the third step, the liquid carbon-based fertilizer and the soil are uniformly mixed and stirred by using a stirrer, wherein the mixing ratio of the liquid carbon-based fertilizer to the soil is 1-8: 1, the rotational speed of the stirrer is set to 80r/min.
Preferably, in the fourth step, the dry yeast is beneficial actinomycete yeast, the adding proportion of the dry yeast is 1-5 per mill, and the rotating speed of the stirrer is 60r/min.
Preferably, in the fifth step, the natural fermentation mode is a composting fermentation mode, a warm and ventilated position is selected for composting, soil or straw is covered on the top and the periphery of the fertilizer pile for sealing and storing for about thirty days.
Preferably, in the seventh step, a microbial cell wall breaking machine is used to break the wall of the soil after the propagation of the microorganisms to release the active spores.
Compared with the prior art, the invention has the beneficial effects that: the invention adopts the liquid carbon-based fertilizer and the actinomycetes to prepare the soil compound enzyme, which can accelerate the metabolism of microorganisms in the soil, activate mineral elements in the soil, avoid the hardening of the soil, generate endogenous hormones, resistant substances and a large amount of organic micromolecules, lighten the unbalance of the proportion of the microorganisms in the soil to a certain extent, effectively inhibit the propagation of fungi in the soil, increase the quantity of beneficial actinomycetes and maintain the balance of the microorganisms in the soil, thereby achieving the aim of ensuring the high yield, the high quality and the health of the soil of crops.
Drawings
FIG. 1 is a flow chart of the method of the present invention.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments, and all other embodiments obtained by those skilled in the art without making creative efforts based on the embodiments of the present invention are included in the protection scope of the present invention.
Referring to fig. 1, an embodiment of the present invention is provided: a method for rapidly preparing soil complex enzyme comprises the steps of firstly, preparing fertilizer; step two, preparing soil; step three, mixing and stirring; step four, strain introduction; step five, natural fermentation; step six, microorganism propagation; step seven, preparing soil complex enzyme;
wherein in the first step, a liquid carbon-based fertilizer is prepared, and the production of the liquid carbon-based fertilizer comprises the following steps: collecting natural organic matters, sorting, removing impurities, pulverizing, degrading, activating, decompressing, blasting, concentrating, and preparing into final product, wherein the natural organic matters comprise crop straw, dry livestock manure or weathered coal, the liquid carbon-based fertilizer contains 72 medium trace elements not less than 20g/L, and the active organic matters (fulvic acid) not less than 120g/L, and N+P 2 O 5 +K 2 O≥200g/L；
In the second step, the no-tillage soil for fallow or the loose and fertile soil with uniform soil structure is selected, and a screening machine is used for simply removing impurities from the soil, such as stones, residual garbage and the like which possibly exist in the soil;
in the third step, the liquid carbon-based fertilizer prepared in the first step and the soil in the second step are mixed and stirred uniformly; uniformly mixing and stirring the liquid carbon-based fertilizer and the soil by using a stirrer, wherein the mixing ratio of the liquid carbon-based fertilizer to the soil is 1-8: 1, setting the rotating speed of a stirrer to 80r/min;
in the fourth step, beneficial actinomycete yeast is selected as the dry yeast, the dry yeast is added into the soil obtained in the third step for mixing and stirring, the adding proportion of the dry yeast is 1-5 per mill, and the rotating speed of the stirrer is 60r/min;
in the fifth step, a proper position is selected, the mixture in the fourth step is naturally fermented, a composting fermentation mode is selected in a natural fermentation mode, a warm and ventilated position is selected for composting, soil or straw is covered on the top and the periphery of the fertilizer pile for sealing and storing for about thirty days;
in the sixth step, the microorganisms in the fifth step are propagated;
in the seventh step, the microorganism in the sixth step is broken, and the microorganism cell wall breaking machine is used for breaking the microorganism wall of the soil after the microorganism propagates, so as to release the active spores, and the soil complex enzyme is prepared.
Based on the above, the invention has the advantages that: the invention adopts the liquid carbon-based fertilizer and actinomycetes yeast to prepare the soil complex enzyme, which can accelerate the metabolism of microorganisms in the soil, activate mineral elements in the soil, avoid soil hardening, effectively inhibit the propagation of fungi in the soil, increase the quantity of beneficial actinomycetes, maintain the balance of the microorganisms in the soil, generate endogenous hormones, resistant substances and a large quantity of small organic molecules in the soil, relieve the occurrence of barrier factors with the continuously increased quantity of the fungi in agricultural production, balance the proportion of three large microorganism groups in the soil, and lighten the imbalance condition of the proportion of the microorganisms in the soil to a certain extent, thereby improving the productivity of the soil and the quality of produced crops.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (8)
1. A method for rapidly preparing soil complex enzyme comprises the steps of firstly, preparing fertilizer; step two, preparing soil; step three, mixing and stirring; step four, strain introduction; step five, natural fermentation; step six, microorganism propagation; step seven, preparing soil complex enzyme; the method is characterized in that:
wherein in the first step, a liquid carbon-based fertilizer is prepared;
in the second step, no-tillage soil for no tillage or loose and fertile soil with uniform soil structure is selected;
in the third step, the liquid carbon-based fertilizer prepared in the first step and the soil in the second step are mixed and stirred uniformly;
in the fourth step, dry yeast is added into the soil obtained in the third step for mixing and stirring;
in the fifth step, a proper position is selected, and the mixture in the fourth step is naturally fermented;
in the sixth step, the microorganisms in the fifth step are propagated;
in the seventh step, the microorganism in the sixth step is broken to prepare the soil complex enzyme.
2. The method for rapidly preparing soil complex enzyme according to claim 1, wherein: in the first step, the production of the liquid carbon-based fertilizer comprises the following steps: collecting natural organic matters, sorting, removing impurities, pulverizing, degrading, activating, decompressing, blasting, concentrating, and preparing into the final product, wherein the natural organic matters comprise crop straw, dry livestock manure or weathered coal.
3. A method for rapidly preparing soil complex enzyme according to claim 2, wherein: in the first step, 72 medium trace elements in the liquid carbon-based fertilizer are more than or equal to 20g/L, and active organic matters (fulvic acid) are more than or equal to 120g/L, and N+P is contained 2 O 5 +K 2 O≥200g/L。
4. The method for rapidly preparing soil complex enzyme according to claim 1, wherein: in the second step, after proper soil is selected, a screening machine is used for simply removing impurities from the soil, and the impurities such as stones and residual garbage possibly existing in the soil are removed.
5. The method for rapidly preparing soil complex enzyme according to claim 1, wherein: in the third step, a stirrer is used for uniformly mixing and stirring the liquid carbon-based fertilizer and the soil, wherein the mixing ratio of the liquid carbon-based fertilizer to the soil is 1-8: 1, the rotational speed of the stirrer is set to 80r/min.
6. The method for rapidly preparing soil complex enzyme according to claim 1, wherein: in the fourth step, beneficial actinomycete yeast is selected as the dry yeast, the adding proportion of the dry yeast is 1-5 per mill, and the rotating speed of the stirrer is 60r/min.
7. The method for rapidly preparing soil complex enzyme according to claim 1, wherein: in the fifth step, a composting fermentation mode is selected as a natural fermentation mode, a warm and ventilated position is selected for composting, soil or straw is covered on the top and the periphery of the fertilizer pile for sealing and storing for about thirty days.
8. The method for rapidly preparing soil complex enzyme according to claim 1, wherein: in the seventh step, a microbial cell wall breaking machine is used for breaking the wall of the soil after the propagation of the microorganisms to release the active spores.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310433348.9A CN116218797A (en) | 2023-04-21 | 2023-04-21 | Method for rapidly preparing soil complex enzyme |
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| CN202310433348.9A CN116218797A (en) | 2023-04-21 | 2023-04-21 | Method for rapidly preparing soil complex enzyme |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116445351A (en) * | 2023-04-21 | 2023-07-18 | 西北农林科技大学 | Method for rapidly culturing indigenous microorganisms |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116445351A (en) * | 2023-04-21 | 2023-07-18 | 西北农林科技大学 | Method for rapidly culturing indigenous microorganisms |
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