CN116211922B - Compound essential oil for aiding sleep and soothing nerves as well as preparation method and application thereof - Google Patents
Compound essential oil for aiding sleep and soothing nerves as well as preparation method and application thereof Download PDFInfo
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- A61K36/18—Magnoliophyta (angiosperms)
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- A61K36/61—Myrtaceae (Myrtle family), e.g. teatree or eucalyptus
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- A—HUMAN NECESSITIES
- A47—FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
- A47G—HOUSEHOLD OR TABLE EQUIPMENT
- A47G9/00—Bed-covers; Counterpanes; Travelling rugs; Sleeping rugs; Sleeping bags; Pillows
- A47G9/02—Bed linen; Blankets; Counterpanes
- A47G9/0238—Bed linen
- A47G9/0253—Pillow slips
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- A—HUMAN NECESSITIES
- A47—FURNITURE; DOMESTIC ARTICLES OR APPLIANCES; COFFEE MILLS; SPICE MILLS; SUCTION CLEANERS IN GENERAL
- A47G—HOUSEHOLD OR TABLE EQUIPMENT
- A47G9/00—Bed-covers; Counterpanes; Travelling rugs; Sleeping rugs; Sleeping bags; Pillows
- A47G9/02—Bed linen; Blankets; Counterpanes
- A47G9/0238—Bed linen
- A47G9/0261—Blanket or duvet enclosing covers, e.g. bag-like
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/63—Oleaceae (Olive family), e.g. jasmine, lilac or ash tree
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- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A61K36/738—Rosa (rose)
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- A—HUMAN NECESSITIES
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- A61K9/10—Dispersions; Emulsions
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/20—Hypnotics; Sedatives
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention provides a compound essential oil for aiding sleep and soothing nerves, and a preparation method and application thereof. The preparation method comprises the following steps of: butyl essential oil, fructus forsythiae essential oil, rose essential oil and jasmine essential oil. The compound essential oil is prepared by utilizing the compatibility principle of monarch, minister, assistant and guide of traditional Chinese medicines, selecting clove essential oil as monarch drug, weeping forsythiae essential oil as ministerial drug, rose essential oil as assistant and jasmine essential oil as guide, and preparing the compound essential oil for assisting sleep and soothing nerves. The preparation method comprises the step of uniformly mixing the preparation raw materials according to the proportion. The essential oil provided by the invention is prepared by extracting plant essential oil containing natural active ingredients, and has the advantages of abundant raw materials and scientific formula ratio. Experimental study shows that the effects of improving sleep and soothing nerves are remarkable.
Description
Technical Field
The invention belongs to the field of traditional Chinese medicine compositions, and particularly relates to a compound essential oil for aiding sleep and soothing nerves, and a preparation method and application thereof.
Background
In recent years, with the increasing intensity of social competition, people bear pressure caused by various events from psychology and society, and often feel excessive, mood depression and anxiety, and insomnia is caused. Modern researches have shown that the pathogenesis of insomnia mainly comprises dysfunction of HPA axis, reduced GABA expression level, reduced melatonin level and the like. When GABA content level in body is low or GABA function is abnormal, the process of participating in sleep initiation and maintenance is weakened, so that the function of a wake-up bolus is enhanced, the central nervous inhibition effect is weakened, and arousal is induced. The essence of insomnia is considered to be the deficiency and excess of the essence, and is mostly related to viscera dysfunction such as liver, heart, spleen, kidney and the like, heart-mind malnutrition or heart-mind difficulty in tranquilization, and viscera imbalance caused by various reasons is an important factor causing insomnia. Whereas insomnia with disharmony between heart and kidney is common in people, its etiology is excessive thought, temperament and interest depression and fire. Excessive heart fire can cause downward loss of kidney water, loss of nourishment of kidney yin, and failure of kidney yin to control heart fire. Heart fire can not descend to the kidneys, kidney water can not enter the heart, and heart-kidney coordination is abnormal, so that vexation and insomnia are caused. The therapeutic principle should nourish yin and reduce fire, and transport heart and kidney.
At present, the treatment means aiming at insomnia is mainly drug treatment, namely hypnotic drugs or sedatives are taken. Although hypnotics or sedatives have sedative effects on the central nervous system of the brain, the drugs are short-lived and need to be taken for a long time, which can cause serious side effects on the body. In addition, hypnotics enter the human body through oral administration, enter blood after being digested in stomach, enter the brain through blood circulation, can exert the drug effect, and have great hysteresis in the whole long time.
Aromatic essential oils are volatile materials extracted from aromatic plant processing. Not only has aromatic smell, but also has a unique transnasal delivery way into the brain. Volatile small molecules in the aromatic odor can be absorbed by nasal mucosa tissues and enter the body, and the information is directly transmitted to the brain, so that the emotion and the physiological function of the body are regulated through the limbic system of the brain. Research shows that many essential oils have the effects of improving sleep and soothing nerves, for example, lavender essential oil can shorten the sleep latency of mice and prolong the sleep duration of mice. The compound essential oil provided by the invention can improve insomnia caused by heart-kidney disharmony, is safe in source, quick in absorption, free of side effects, convenient to use and capable of meeting the requirements of users.
Disclosure of Invention
The invention aims to provide compound essential oil with definite effect and capable of improving vexation and insomnia as well as a preparation method and application thereof. The essential oil provided by the invention is prepared by extracting plants containing natural active ingredients, has scientific formula proportion, has obvious sleep-aiding and nerve-soothing effects, and can effectively relieve insomnia symptoms.
The first aspect of the invention discloses compound essential oil for improving vexation and insomnia, which comprises the following preparation raw materials: butyl essential oil, fructus forsythiae essential oil, rose essential oil and jasmine essential oil.
In some embodiments, the preparation materials include the following parts by weight: 1-10 parts of clove essential oil, 1-10 parts of weeping forsythia essential oil, 0.5-5 parts of rose essential oil and 0.5-5 parts of jasmine essential oil.
In some embodiments, the preparation materials include the following parts by weight: 1-5 parts of butane essential oil, 2-6 parts of weeping forsythiae essential oil, 0.5-2 parts of rose essential oil and 0.5-2 parts of jasmine essential oil.
In some embodiments, the preparation materials include the following parts by weight: 4 parts of clove essential oil, 6 parts of weeping forsythia essential oil, 1 part of rose essential oil and 1 part of jasmine essential oil.
The second aspect of the invention provides a preparation method of the compound essential oil, which is characterized in that the preparation raw materials are uniformly mixed.
The third aspect of the invention provides a compound essential oil, which is characterized by being prepared according to the preparation method.
The fourth aspect of the invention provides application of the compound essential oil, which is characterized in that the compound essential oil is used for improving heart-kidney disharmony type insomnia.
The fifth aspect of the invention provides application of the compound essential oil in preparing tranquilization medicines or sanitary products. The method is characterized in that: the tranquillizing medicament is a spray composed of essential oil of traditional Chinese medicine, or is a civil sanitary product which is prepared by taking the essential oil as an active ingredient, adding conventional auxiliary materials and adopting a conventional preparation process of traditional Chinese medicine, and comprises a pillowcase, a quilt cover and the like.
The sixth aspect of the invention provides a tranquilizer, which is characterized by comprising the compound essential oil. The tranquilizer can also comprise pharmaceutically acceptable auxiliary materials.
Compared with the prior art, the invention has the following technical advantages:
(1) The essential oil provided by the invention is prepared by extracting plant essential oil containing natural active ingredients, and has the advantages of abundant raw materials and scientific formula ratio.
(2) Flos Caryophylli in the compound essential oil is used as a monarch drug for nourishing kidney deficiency, guiding kidney yin upwards and filling heart fire; fructus forsythiae is used as ministerial drug, has the effects of expelling pathogenic cold and cool, clearing heat and purging pathogenic fire, and inducing heart-yang to lower the kidney-water, and can achieve the state of yin-yang balance and water-fire balance; and the rose is added as an adjuvant drug to promote qi circulation and remove depression, and the jasmine regulates qi and harmonizes the middle warmer, so that the qi movement is achieved. The whole formula can nourish yin and reduce fire, traffic heart and kidney, and play a role in helping sleep and tranquillizing.
(3) Experimental study shows that the compound essential oil provided by the invention has a protective effect on PC12 nerve cells damaged by corticosterone, can obviously improve the expression quantity of GABA, and can obviously shorten the sleep latency of mice.
(4) The preparation method is simple, does not need a complex preparation process, and is economical and environment-friendly.
(5) The compound essential oil provided by the invention is safe in source, quick in absorption and remarkable in effect, and can meet the requirements of users.
Drawings
FIG. 1 is a data graph of the effect of compound essential oil on stress-induced neuronal damage;
FIG. 2 is a data graph of the effect of jasmine monosodium glutamate oil on stress-induced neuronal damage;
FIG. 3 is a data graph showing the effect of rose monosodium glutamate on nerve cell damage caused by stress;
FIG. 4 is a graph of data relating to the effect of compound essential oils on GABA expression;
FIG. 5 is a graph showing the effect of compound essential oil on sleep latency in a sleep mouse with a threshold dose of sodium pentobarbital;
Compared to the model group, (#P < 0.01);
Compared with the control group, the P is less than 0.05, P is less than 0.01, P is less than 0.001.
Detailed Description
The invention is further illustrated by means of the following examples, which are not intended to limit the scope of the invention. The experimental methods, in which specific conditions are not noted in the following examples, were selected according to conventional methods and conditions, or according to the commercial specifications.
Ding Xiangwei pungent and warm in nature, enter spleen, stomach, lung and kidney meridians, and Caryophylli flos can warm kidney and strengthen yang, nourish kidney deficiency, induce kidney yin to ascend and perfuse heart fire. Researches show that the clove volatile oil has a stimulation effect on the brain, and can be used for treating insomnia and relieving anxiety and depressed emotion.
Lian Qiao is bitter in flavor and slightly cold in nature, enters lung and heart meridians, and can clear heart heat and treat symptoms such as excessive heart fire, dysphoria with smothery sensation in the chest, palms and soles.
The rose is sweet and slightly bitter in taste, warm in nature, and enters liver and spleen channels, and can sooth liver, promote qi circulation, relieve depression, smooth qi movement and relieve anxiety and depressed emotion. Studies show that citronellol and geraniol in the rose essential oil can increase the sleep duration of mice, and have sedative and hypnotic effects.
Jasmine is pungent and slightly sweet in flavor, warm in nature, and enters liver, spleen and stomach meridians, and can regulate qi, resolve depression and harmonize middle energizer, smooth qi movement, dredge liver qi and improve emotion. Research shows that the jasmine essential oil can improve the central nervous system function and obviously relieve the adverse symptoms such as neurasthenia, insomnia, dreaminess, anxiety, depression and the like.
Example 1
Mixing 5 parts by weight of butyl essential oil, 6 parts by weight of fructus forsythiae essential oil, 1 part by weight of rose essential oil and 1 part by weight of jasmine essential oil, and diluting 1000-2000 parts of 95% ethanol uniformly to obtain the compound essential oil.
Example 2
Mixing 5 parts by weight of butyl essential oil, 6 parts by weight of fructus forsythiae essential oil, 1 part by weight of rose essential oil and 1 part by weight of jasmine essential oil, and diluting with 1000-2000 parts of water to obtain the compound essential oil.
Example 3
Mixing 4 parts by weight of butyl essential oil, 5 parts by weight of fructus forsythiae essential oil, 0.5 part by weight of rose essential oil, 0.5 part by weight of jasmine essential oil, and 1000-2000 parts of 95% ethanol, and uniformly mixing to obtain the compound essential oil.
Example 4
Compound essential oil efficacy test:
(1) The compound essential oil has the protective effect on nerve cell injury caused by stress:
PC12 cells in the logarithmic growth phase were inoculated into 96-well plates at a cell density of about 2X 10 4. Multidot.mL －1 cell suspension and cultured. After cells were attached to the surface of the culture medium after culturing at 37℃for 24 hours under 5% CO 2, the original culture medium was discarded. Cells were divided into control, model and dosing groups. The control group was cultured by adding 100. Mu.L of medium, the model group was cultured by adding 100. Mu.L of medium containing －1 corticosterone at a final concentration of 250. Mu. Mol.L, and the drug administration group was cultured by adding 100. Mu.L of medium containing different concentrations of drug solution, each group was provided with 3 parallel wells, and MTT assay was performed.
The experimental results are shown in figures 1-3, the compound essential oil can protect cells when diluted by 10-256 times in the concentration of the original formula, and the drug effect is obvious.
(2) Real-time fluorescent quantitative PCR (RT-qPCR) analysis of the effect of the compound essential oil of the invention on GABA expression:
The PC12 cell suspension in the logarithmic growth phase with the density of 4X 10 5. Mu.L －1 was inoculated into 6-well plates at 100. Mu.L per well and cultured for 24 hours. 2mL of complete medium is added to the control group, 2mL of complete medium solution containing 250 mu mol.L －1 is added to the model group, and 2mL of complete medium solution containing 250 mu mol.L －1 and 2mL of essential oil compositions with different concentrations (the concentration is 1/8, 1/12 and 1/16 of the original composition) are added to the administration group. After 48h, 1mL of PBS was added to each well and washed 2 times, 1mL of Trizol solution was used to lyse the cells, and the cells were extracted with chloroform and allowed to stand at room temperature for 10min. Centrifuge at 12000rpm at 4℃for 15min. The cell supernatant was aspirated and the RNA sample was precipitated by adding the same volume of isopropanol. Washing with 80% ethanol for 2 times, and volatilizing the residual ethanol. 20. Mu.L of DEPC water was added thereto for solubilization, and quantitative nucleic acid detection was performed. Reverse transcription was performed using PRIMESCRIPT II first strand cDNA Synthesis kit. The expression level of the gene was quantified using SYBR premix Ex Taq reagents. The relative folding changes in gene expression were calculated using the 2- △△Ct method with GAPDH gene expression levels as an endogenous control. The primer sequences are shown in Table 1.
TABLE 1 fluorescent quantitative RT-qPCR primer sequence information
The experimental results are shown in fig. 4, and the compound essential oil can obviously improve the expression quantity of GABA after being diluted by 12-16 times in the original formula.
(3) The influence of the compound essential oil on the sleep latency of a sleeping mouse with a threshold dose of sodium pentobarbital:
Kunming mice weighing 18-22g were selected and grouped into 4 groups of 5 mice each. After the mice are normally raised for four days, starting an experiment, weighing and recording the mice before the experiment, wherein the first group is a control group, and the mice in the group are filled with distilled water of 0.1 mL/kg －1 each day and inhale 1% of Tween-80 solution for 30min; the second group is semen Ziziphi Spinosae group, and the group of mice is irrigated with 234 mg/kg －1 semen Ziziphi Spinosae solution every day and inhaled with 1% Tween-80 solution for 30min; the third group is lavender essential oil group, the mice of the group are irrigated with 0.1 mL/kg －1 distilled water every day and inhale 2 mg/kg －1 lavender essential oil for 30min; the fourth group is compound essential oil group, and the mice of the group are irrigated with 0.1 mL/kg －1 distilled water daily and inhale 2 mg/kg －1 compound essential oil for 30min for 7 days. After the end of the last 1 day of administration, 45 mg.kg －1 pentobarbital sodium solution was injected into the abdominal cavity of each group of mice, the mice after injection were individually placed into a feeding box, the feeding box was numbered, and the time when the specular reflection of each mouse disappeared for 1min was recorded.
As shown in the experimental result in figure 5, compared with a control group, the administration group can obviously shorten the sleep latency of mice, and in the administration group, the compound essential oil group obtained by the technical scheme of the application has shorter sleep latency compared with the wild jujube seed group and the lavender essential oil group, so that the essential oil component in the compound essential oil group has a compound effect, and has better sleep-aiding and nerve-soothing effects compared with a single essential oil component.
The embodiments of the present invention have been described in detail above with reference to the accompanying drawings, but the present invention is not limited to the described embodiments. It will be apparent to those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, and yet fall within the scope of the invention.
Claims (7)
1. The compound essential oil for aiding sleep and soothing nerves is characterized by being prepared from the following raw materials in parts by weight: 1-10 parts of clove essential oil, 1-10 parts of weeping forsythia essential oil, 0.5-5 parts of rose essential oil and 0.5-5 parts of jasmine essential oil.
2. The compound essential oil according to claim 1, which is characterized by being prepared from the following raw materials in parts by weight: 1-5 parts of butane essential oil, 2-6 parts of weeping forsythiae essential oil, 0.5-2 parts of rose essential oil and 0.5-2 parts of jasmine essential oil.
3. The compound essential oil according to claim 1, which is characterized by being prepared from the following raw materials in parts by weight: 4 parts of clove essential oil, 6 parts of weeping forsythia essential oil, 1 part of rose essential oil and 1 part of jasmine essential oil.
4. A method for preparing the compound essential oil according to any one of claims 1 to 3, wherein the preparation raw materials are uniformly mixed.
5. The application of the compound essential oil according to any one of claims 1-3 in preparing tranquilizer or sanitary products, wherein the tranquilizer is a spray prepared from the compound essential oil, or a pillowcase or quilt cover of civil sanitary products prepared by adding conventional auxiliary materials and according to the conventional preparation process of traditional Chinese medicines.
6. A tranquilizer characterized in that the active ingredient is the compound essential oil according to any one of claims 1-3.
7. The tranquilizer of claim 6, further comprising a pharmaceutically acceptable excipient.
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