CN105287723B - A kind of hiding schizonepeta compound essential oil and preparation method thereof - Google Patents
A kind of hiding schizonepeta compound essential oil and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of with blood pressure lowering, refreshes the mind, relieves fatigue and the hiding schizonepeta compound essential oil of antidepressant effect, by weight, raw material includes: Herba Schizonepetae volatile oil 30-50 parts, 10-20 parts of rhododendron anthopogonoide volatile oil, 10-20 parts of Carum carvi volatile oil, 10-20 parts of Herba Dracocephali Tangutici volatile oil, 10-20 parts of hyssop volatile oil of hiding.Hiding schizonepeta compound essential oil of the invention can be relieved the symptoms such as the tired, lassitude of hypertensive patient's giddy, meanwhile, it can also reduce blood pressure, and there is antidepressant effect.
Description
Technical field
The invention belongs to hide schizonepeta product deep process technology field, more particularly to a kind of hiding schizonepeta compound essential oil and its system
Preparation Method.
Background technique
Volatile oil is also known as essential oil, essential oil or aromatic oil, is present in glandular hairs, oil pipe, grease chamber, resin canal or the secretion of plant
In the histoorgans such as cell, be it is a kind of have special odor, molecular weight smaller, can be steamed with vapor under normal temperature condition and with
The general name of the unmixing volatility oily ingredient of water.Volatile oil is mixture, and the composition of specific ingredient is complex, a plant
The volatile oil component of object can by ten it is several to hundreds of at being grouped as.In these chemical components, terpenoid is the most often
See, mainly includes monoterpene, sequiterpene and its containing oxygen derivative, wherein the most bioactivity of containing oxygen derivative is relatively strong or has virtue
Fragrance taste.Aromatic compound is the major class compound in chemical composition of volatile oil, is only second to terpene, mostly oxygen-containing derivative
Object, such as vanillic aldehyde, salicylic acid.In addition, also containing small molecule aliphatic character in volatile oil, there is volatility, such as octene, just
Heptane, isopentyl aldehyde etc..Research and practice have shown that, volatile oil has antibechic, relievings asthma, is eliminating the phlegm, antipyretic-antalgic, wind dispelling, spasmolysis, strong
The effects of stomach, antibacterial anti-inflammatory, antiallergy, expelling parasite, calmness, restoring consciouness.
In recent years, with Chinese society and economic fast development, the disease incidence of the chronic diseases such as hypertension is risen with it,
And patient groups tend to rejuvenation, and this kind of crowd is mostly family, office, due to bending over one's desk working for a long time, is easy to appear spirit not
The symptoms such as vibration, tired, the dizzy amnesia of body, will alleviate these symptoms, improve work, learning efficiency, caffeine class product is normal
The selection seen, still, due to the particularity of Hypertensive Population, doping class product can cause unusual fluctuations, very to blood pressure
To there are the symptoms such as insomnia, anxiety, headache, palpitaition, tachycardia, the health of hypertensive patient is caused further to damage,
Therefore, Hypertensive Population should be avoided using caffeine class product.And other products that refresh the mind in the market, such as traditional Chinese medicine mouth system of mourning
Agent, although having no adverse effects to blood pressure, action speed is slower, can not play the purpose to refresh the mind in a short time；And
Relevant essential oil class product, although can also play the role of refreshing the mind, action time is shorter, and what has influence blood pressure
Also unknown, it can be used for Hypertensive Population, also do not have experiment to investigate and verify it.Therefore, for Hypertensive Population, one
The product that money can refresh the mind, relieves fatigue and reduce blood pressure is the production that this kind of crowd especially office crowds need
Product.
Summary of the invention
The present invention provides a kind of compound essential oils containing hiding Herba Schizonepetae volatile oil.Solution of the invention is based on Tibetan medicine
The correlation theory of medicine, and the Tibetan medicine and pharmacology ancient books and records such as the Four-Volume Medical Code, " Jingzhubencao " are referred to, it is ground in conjunction with modern biology, pharmacy
Study carefully achievement, it is preferred by many experiments, hiding Herba Schizonepetae volatile oil, rhododendron anthopogonoide volatile oil, Carum carvi volatile oil, Herba Dracocephali Tangutici are waved
Five kinds of volatile oil such as hair oil, hyssop volatile oil pass through rational proportion, obtain the compound essential oil containing hiding Herba Schizonepetae volatile oil.Pass through
Relevant effect experiment verifying, compound essential oil of the present invention has the effect that reduces blood pressure significantly, and can play and relieve fatigue, refresh oneself
The effect of restoring consciouness is suitable for Hypertensive Population and uses.In addition, the present invention is in many experiments preferred process, it has surprisingly been found that this
Invention compound essential oil has certain antidepressant effect, can be used for the prevention and treatment of depression.
In order to achieve the above-mentioned object of the invention, technical scheme is as follows:
It is a kind of with blood pressure lowering, refresh the mind, relieve fatigue and the hiding schizonepeta compound essential oil of antidepressant effect, by weight
Part meter, raw material include: Herba Schizonepetae volatile oil 30-50 parts, 10-20 parts of rhododendron anthopogonoide volatile oil, Carum carvi volatile oil 10-20 of hiding
Part, 10-20 parts of Herba Dracocephali Tangutici volatile oil, 10-20 parts of hyssop volatile oil.
Wherein, the preparation method that Herba Schizonepetae volatile oil is hidden in the raw material components is: hiding schizonepeta herb being taken to be ground into coarse powder
Afterwards, 12 times of amount water are added, impregnate 1h, 6h, 2~3h of settle and separate in oil water separator are extracted using steam distillation method
Afterwards, it collects and obtains hiding Herba Schizonepetae volatile oil.
Wherein, the preparation method of rhododendron anthopogonoide volatile oil is in the raw material components: taking rhododendron anthopogonoide and leaf, crushes
After coarse powder, 10 times of amount water are added, impregnate 1h, 8h, settle and separate 2 in oil water separator are extracted using steam distillation method
After~3h, collection obtains rhododendron anthopogonoide volatile oil.
Wherein, the preparation method of Carum carvi volatile oil is in the raw material components: Carum carvi fruit being taken to be ground into coarse powder
Afterwards, 8 times of amount water are added, impregnate 1h, 4h is extracted using steam distillation method, in oil water separator after 2~3h of settle and separate,
Collection obtains Carum carvi volatile oil.
Wherein, the preparation method of Herba Dracocephali Tangutici volatile oil is in the raw material components: Herba Dracocephali Tangutici herb being taken to be ground into
After coarse powder, be added 10 times of amount water, impregnate 2h, 8h is extracted using steam distillation method, in oil water separator settle and separate 2~
After 3h, collection obtains Herba Dracocephali Tangutici volatile oil.
Wherein, the preparation method of hyssop volatile oil is in the raw material components: hyssop herb being taken to be ground into coarse powder
Afterwards, 8 times of amount water are added, impregnate 2h, 6h is extracted using steam distillation method, in oil water separator after 2~3h of settle and separate,
Collection obtains hyssop volatile oil.
Above-mentioned compound essential oil preparation process: Herba Schizonepetae volatile oil, rhododendron anthopogonoide volatile oil, Carum carvi volatile oil, Gan Qing will be hidden
Green orchid volatile oil, hyssop volatile oil are got all the ready by said ratio, are sequentially placed into stirring container, and whipping temp is 20~25 DEG C,
80~100r/min of mixing speed, 30~60min of equidirectional stirring, make each raw material be sufficiently mixed uniformly, packing, seal to get
To finished product.
Application method:
(1) champignon uses: by compound essential oil of the present invention, according to every 5 cubic metres using 1-3 drop, using heat source type
Scent diffusion equipment carries out aroma diffusing.
(2) spraying to use: by compound essential oil of the present invention, 10- is added according to every 100 milliliters of pure water or distilled water
30 drops, sufficiently shake up, and carry out spraying diffusion.
(3) massage uses: compound essential oil of the present invention is added according to matrix such as every 10 milliliters of base oils or lotions
5-10 drop, mixes well, and carries out skin massaging, is preferred with the massage of forehead position.
(4) dipping bath: taking 20-30 drop essential oil to be put into the bathtub full of warm water, impregnates 15-20 minutes.
The effect of primary raw material in invention:
Hiding schizonepeta is the herb that Lamiaceae plant hides schizonepeta Nepeta angustifolia C.Y.Wu.It originates in Tibet
(Jiangzi, Lhasa) is the distinctive Tibetan medicine material in Tibet.Hiding schizonepeta be clinically mainly used for coma convulsion faint, apoplexy, epilepsy, brain overflow
Blood, sore injure the treatment of the diseases such as pain, have good curative effect." Jingzhubencao " is pointed out: this product " controls epilepsy, cerebral hemorrhage, sore wound
And the diseases such as pain ", also there are " Tibetan medicine will ", " medicine name is assembled " hiding schizonepeta treatment apoplexy, evil disease, pleurisy and dermopathic record.
Modern studies have found that hiding schizonepeta can play inducing resuscitation of having one's ideas straightened out effect, have with the volatile oil contained by it compared with Important Relations, it therefore, can
Consider the product for being used to refresh the mind by its volatile oil.
Rhododendron anthopogonoide is Maxim. maos of larynxs of ericad rhododendron anthopogonoide Rhododendron anthopogonoides
Cuckoo Rhododendron cephalanthum Franch. and flower-shaped cuckoo Rhododendron of heralding spring
The dry flower and leaf of primulaeflarum Bur.et Franch..It is mild-natured, it is sweet in flavor, puckery, there is clearing heat for detumescence, the work of kidney tonifying
With.Rhododendron anthopogonoide has unique fragrance, contains that a large amount of volatile oil is related with it, the study found that the volatile oil of rhododendron anthopogonoide
In, with the content highest of benzylacetone.Rhododendron anthopogonoide volatile oil has antibechic, relievings asthma, is antibacterial, relieving fatigue, improving resist oxygen lack
The active function of ability etc..
Carum carvi is also referred to as Caraway, is the fruit of umbelliferae Caraway Carum carvi L..It is warm-natured, acrid flavour,
With qi-regulating, analgesic, the effect of removing toxic substances.Volatile oil is the main active principle of Carum carvi, wherein Eucarvone, limonene, dihydro
Eucarvone etc. is the main component of volatile oil.The study found that Carum carvi volatile oil it is anti-oxidant, it is antibacterial, aid digestion, relieving cough and asthma,
The effects of relieving pain.In addition, Carum carvi volatile oil has refrigerant spearmint, Xin Xiang and the medicine gas with pine forest sample graceful bearing
Perfume (or spice), gustation are characterized in refrigerant, the celery sample flavor with radix aucklandiae charm of the faint scent of sweet tea, spearmint, have and adjust nerve, relax
Decompression force, the effect relieved fatigue.
Herba Dracocephali Tangutici is Labiatae Herba Dracocephali Tangutici Dracocephalum tanguticum Maxim.var tanguticum
Dry aerial parts.It is cold in nature, it is sweet in flavor, bitter, have the function of soothing the liver stomach function regulating, clearing away heat and promoting diuresis, relieving cough and reducing sputum.Its volatile oil has
Relieve fatigue, anti anoxia, it is relieving cough and asthma the effects of.
Hyssop is on the dry ground of Lamiaceae plant hyssop Hyssopus officinalis Var.decumbens
Part.Hyssop volatile oil mainly contains the ingredients such as borneol, camphor, radix achyranthis bidentatae ketone, linalool.Hyssop volatile oil has suppression
The effects of bacterium, anti-spasm, antibechic, calmness, moreover it is possible to make one the effect that brains is clear, relieves fatigue.
The object of the present invention is to provide a kind of compound essential oil containing hiding Herba Schizonepetae volatile oil, which is waved by hiding schizonepeta
The composition such as hair oil, rhododendron anthopogonoide volatile oil, Carum carvi volatile oil, hyssop volatile oil, several volatile oil effects cooperate, phase
It is auxiliary to coordinate, there is apparent blood pressure lowering, refresh the mind, relieve fatigue and antidepressant effect.
The invention has the following advantages that
(1) plant volatile oils such as schizonepeta, rhododendron anthopogonoide, Carum carvi, Herba Dracocephali Tangutici, hyssop will be hidden, with Tibetan medicine and pharmacology tradition
Based on theory, and the above-mentioned plant volatile oil side of being combined into is given full play to the spy of each volatile oil by reference for modern biotechnology
Point has blood pressure lowering, refreshes the mind, relieves fatigue, antidepressant effect.
(2) product of the present invention is used especially suitable for Hypertensive Population, and especially alleviation hypertensive patient's giddy is tired, smart
The mind symptoms such as depressed, meanwhile, it can also reduce blood pressure, maintain the stabilization of hypertensive patient's blood pressure.
(3) for the present invention in experiment preferred process, having been surprisingly found that also has antidepressant effect, can rise to the treatment of depression
To certain effect.
(4) the used raw material of the present invention is plant volatile oil, and permeability is strong, can quickly be absorbed by body, can be rapidly
Play its drug action.
(5) simple production process of the present invention requires equipment requirement lower, suitable large-scale production popularization from now on.
Specific embodiment
Technical solution of the invention is further illustrated combined with specific embodiments below, these embodiments should not be understood as
It is the limitation to technical solution.
Embodiment 1
It hides the preparation of Herba Schizonepetae volatile oil: after taking hiding schizonepeta herb to be ground into coarse powder, 12 times of amount water is added, impregnate 1h, use
Steam distillation method extracts 6h, and in oil water separator after 2~3h of settle and separate, collection obtains hiding Herba Schizonepetae volatile oil.
The preparation of rhododendron anthopogonoide volatile oil: taking rhododendron anthopogonoide and leaf, and after being ground into coarse powder, 10 times of amount water are added, and impregnates
1h extracts 8h using steam distillation method, and in oil water separator after 2~3h of settle and separate, collection obtains rhododendron anthopogonoide and waves
Hair oil.
The preparation of Carum carvi volatile oil: after taking Carum carvi fruit to be ground into coarse powder, 8 times of amount water are added, 1h are impregnated, using water
Steam distillation method extracts 4h, and in oil water separator after 2~3h of settle and separate, collection obtains Carum carvi volatile oil.
The preparation of Herba Dracocephali Tangutici volatile oil: after taking Herba Dracocephali Tangutici herb to be ground into coarse powder, being added 10 times of amount water, impregnate 2h,
8h is extracted using steam distillation method, in oil water separator after 2~3h of settle and separate, collection obtains Herba Dracocephali Tangutici volatilization
Oil.
The preparation of hyssop volatile oil: after taking hyssop herb to be ground into coarse powder, 8 times of amount water are added, 2h are impregnated, using water
Steam distillation method extracts 6h, and in oil water separator after 2~3h of settle and separate, collection obtains hyssop volatile oil.
Take hiding Herba Schizonepetae volatile oil 300g, rhododendron anthopogonoide volatile oil 200g, Carum carvi volatile oil 200g, Herba Dracocephali Tangutici volatile oil
200g, hyssop volatile oil 200g.
By weighed hiding Herba Schizonepetae volatile oil, rhododendron anthopogonoide volatile oil, Carum carvi volatile oil, Herba Dracocephali Tangutici volatile oil, ox
Knee grass volatile oil, is sequentially placed into stirring container, and whipping temp is 25 DEG C, mixing speed 100r/min, equidirectional stirring
60min is sufficiently mixed each raw material uniformly, and packing seals to get finished product is arrived.
Embodiment 2
Hide Herba Schizonepetae volatile oil, rhododendron anthopogonoide volatile oil, Carum carvi volatile oil, Herba Dracocephali Tangutici volatile oil, hyssop volatile oil
Preparation process with embodiment 1.
Take hiding Herba Schizonepetae volatile oil 300g, rhododendron anthopogonoide volatile oil 100g, Carum carvi volatile oil 100g, Herba Dracocephali Tangutici volatile oil
100g, hyssop volatile oil 100g.
By weighed hiding Herba Schizonepetae volatile oil, rhododendron anthopogonoide volatile oil, Carum carvi volatile oil, Herba Dracocephali Tangutici volatile oil, ox
Knee grass volatile oil, is sequentially placed into stirring container, and whipping temp is 20 DEG C, mixing speed 80r/min, equidirectional stirring 30min,
It is sufficiently mixed each raw material uniformly, packing seals to get finished product is arrived.
Embodiment 3
Hide Herba Schizonepetae volatile oil, rhododendron anthopogonoide volatile oil, Carum carvi volatile oil, Herba Dracocephali Tangutici volatile oil, hyssop volatile oil
Preparation process with embodiment 1.
Take hiding Herba Schizonepetae volatile oil 400g, rhododendron anthopogonoide volatile oil 150g, Carum carvi volatile oil 150g, Herba Dracocephali Tangutici volatile oil
150g, hyssop volatile oil 150g.
By weighed hiding Herba Schizonepetae volatile oil, rhododendron anthopogonoide volatile oil, Carum carvi volatile oil, Herba Dracocephali Tangutici volatile oil, ox
Knee grass volatile oil, is sequentially placed into stirring container, and whipping temp is 25 DEG C, mixing speed 90r/min, equidirectional stirring 45min,
It is sufficiently mixed each raw material uniformly, packing seals to get finished product is arrived.
Embodiment 4
Hide Herba Schizonepetae volatile oil, rhododendron anthopogonoide volatile oil, Carum carvi volatile oil, Herba Dracocephali Tangutici volatile oil, hyssop volatile oil
Preparation process with embodiment 1.
Take hiding Herba Schizonepetae volatile oil 350g, rhododendron anthopogonoide volatile oil 200g, Carum carvi volatile oil 200g, Herba Dracocephali Tangutici volatile oil
150g, hyssop volatile oil 100g.
By weighed hiding Herba Schizonepetae volatile oil, rhododendron anthopogonoide volatile oil, Carum carvi volatile oil, Herba Dracocephali Tangutici volatile oil, ox
Knee grass volatile oil, is sequentially placed into stirring container, and whipping temp is 22 DEG C, mixing speed 100r/min, equidirectional stirring
60min is sufficiently mixed each raw material uniformly, and packing seals to get finished product is arrived.
Embodiment 5
Hide Herba Schizonepetae volatile oil, rhododendron anthopogonoide volatile oil, Carum carvi volatile oil, Herba Dracocephali Tangutici volatile oil, hyssop volatile oil
Preparation process with embodiment 1.
Take hiding Herba Schizonepetae volatile oil 450g, rhododendron anthopogonoide volatile oil 100g, Carum carvi volatile oil 150g, Herba Dracocephali Tangutici volatile oil
150g, hyssop volatile oil 150g.
By weighed hiding Herba Schizonepetae volatile oil, rhododendron anthopogonoide volatile oil, Carum carvi volatile oil, Herba Dracocephali Tangutici volatile oil, ox
Knee grass volatile oil, is sequentially placed into stirring container, and whipping temp is 20 DEG C, mixing speed 90r/min, equidirectional stirring 30min,
It is sufficiently mixed each raw material uniformly, packing seals to get finished product is arrived.
Embodiment 6
1 product of schizonepeta compound essential oil embodiment, 2 product of embodiment, 5 product of embodiment are hidden to the shadow of hypertensive rat model
It rings
Sample: hiding 1 product of schizonepeta compound essential oil embodiment, 2 product of embodiment, 5 product of embodiment, positive control are Malaysia
Sour enalapril tablet.
Experimentation and result
Administration mode: being put into Fragrance Lamp pallet in closed experiment box, successively extracts 10mL distilled water and 0.1mL compound essence
Oil is put into pallet.Fragrance Lamp is heated, after essential oil is spread uniformly in box, rat is put into box, sucks essential oil,
Rat is taken out after 30min, it is twice daily, for 4 weeks.Enalapril maleate piece carries out (agent by the way of gastric infusion
Amount: 20mg/kg), it is same for 4 weeks.
Male spontaneous hypertensive rat (SHR) 50, weight 260 ± 20g, SPF grade, male ensive rat
(WKY) 10, weight 260 ± 20g, SPF grade.SHR rat is randomly divided into 5 groups, respectively 1 product group of embodiment, implementation
2 product group of example, 5 product group of embodiment, enalapril maleate group, model group, WKY rat is as Normal group.Rat connects
Continuous administration 4 weeks, measures blood pressure with BP-2006A type intelligence non-invasive blood pressure measuring after stomach-filling 1h on the firstth weekly.Environment quiet, constant temperature
(26 DEG C), in awake and non-measurement rat systolic pressure under enraging state, continuous measurement 3 times takes mean value.After administration terminates for 24 hours, neck
Arterial blood extracting, separates serum, and measurement Endothelin (ET), angiotensinⅡ (Ang II) are horizontal.It the results are shown in Table 1 and table 2.
1 embodiment product of table to SHR rat blood pressure influence (N=10, mmHg)
Note: compared with model group, P < 0.01 * P < 0.05, * *.
2 embodiment product of table to ET, Ang II in SHR rat blood influence (N=10)
Note: compared with model group, P < 0.01 * P < 0.05, * *.
Above-mentioned SHR rat model test result show three embodiment products of the invention have significantly reduce SHR it is big
The effect of mouse blood pressure.Table 1 is the results show that at first week of medication, and the antihypertensive effect of 5 product of embodiment is obvious, systolic pressure and mould
Type group relatively has extremely significant sex differernce (P < 0.01), and diastole is pressed with significant difference (P < 0.05), and embodiment 1 and embodiment 2
Product systolic pressure and model group have extremely significant sex differernce (P < 0.01), and diastolic pressure is although also there is decline, with model group without significant
Sex differernce.Medication second, third, 4th week, the antihypertensive effect continued smooth of 5 product of embodiment, systolic pressure and diastolic pressure
Compared with model group, have extremely significant sex differernce (P < 0.01), embodiment 1,2 product of embodiment antihypertensive effect also persistently show
It is existing, in third week and 4th week, systolic pressure, diastolic pressure compared with model group, have significant (P < 0.05) or extremely significant property (P <
0.01) difference.AngiotensinⅡ be its maintain and increase blood pressure key factor and strongest endogenous vasoconstriction because
One of son.Endothelin is the active peptides with powerful contracting vascular function synthesized by endothelial cell, Endothelin and its receptor knot
It closes, the vessel retraction effect that mediate smooth muscle cell relies on.Therefore, the raising of angiotensinⅡ, level of ET can be led
Blood pressure is caused to increase.Table 2 is the results show that three embodiment products of the invention can reduce SHR rat angiotensin II, Endothelin
Level has significant (P < 0.05) or extremely significant property (P < 0.01) difference, wherein the effect of embodiment 5 is the most compared with model group
Obviously.
Embodiment 7
1 product of schizonepeta compound essential oil embodiment, 2 product of embodiment, 5 product of embodiment are hidden to the shadow of sedative hypnotic effect
It rings
Sample: hiding 1 product of schizonepeta compound essential oil embodiment, 2 product of embodiment, 5 product of embodiment, positive control are coffee
Cause.
Experimentation and result
Administration mode: being put into Fragrance Lamp pallet in closed experiment box, successively extracts 10mL distilled water and 0.1mL compound essence
Oil is put into pallet.Fragrance Lamp is heated, after essential oil is spread uniformly in box, mouse is put into box, sucks essential oil,
Mouse is taken out after 30min, twice daily, continues 14.Caffeine carries out (dosage: 10mg/ by the way of gastric infusion
Kg), equally continue 14.
Take Kunming mouse 50,20 ± 2g of weight, half male and half female is randomly divided into 5 groups: 1 product group of embodiment, embodiment
2 product groups, 5 product group of embodiment, caffeine group, blank control group.It (is infused in abdominal cavity respectively at the 7th, the 14th day ip after 1h is administered
Penetrating) yellow Jackets 50mg/kg with righting reflex loss lmin the above are the index of sleep observes and records the mouse that do not fall asleep
Number of elements (do not fall asleep in 60min in terms of egersis；Calculate egersis rate), latent time (ip yellow Jackets to sleep when
Between), sleeping time (fall asleep to righting reflex reappear time).It the results are shown in Table 3 and table 4.
3 embodiment product of table the 7th day to yellow Jackets hypnotic effect influence ()
Note: compared with blank control group, P < 0.01 * P < 0.05, * *；Compared with caffeine group,ΔP<0.05。
4 embodiment product of table the 14th day to yellow Jackets hypnotic effect influence ()
Note: compared with blank control group, P < 0.01 * P < 0.05, * *；Compared with caffeine group,ΔP<0.05。
Barbiturate is universal central depressant, and with increasing for dosage, the calmness that can sequentially display out is urged
It sleeps, anticonvulsion or anesthetic effect, therefore, yellow Jackets can investigate the effect of refreshing the mind of drug.Experiments indicate that making
With three embodiment products of the invention and caffeine, the hypnotic effect of yellow Jackets can be fought, is using product of the present invention
After caffeine 7 days and 14 days, mice sleep latent time is obviously prolonged, and compared with blank control group, there is extremely significant sex differernce
(P < 0.01), wherein after using 7 days and 14 days, 5 product of embodiment extends mice sleep latent time, with caffeine group ratio
Compared with there is significant difference (P < 0.05)；Using product of the present invention and after caffeine 7 days and 14 days, mouse sleep time is bright
It is aobvious to shorten, compared with blank control group, there is extremely significant sex differernce (P < 0.01) wherein, after using 7 days and 14 days, embodiment 5
Product, which shortens mouse sleep time, has significant difference (P < 0.05) compared with caffeine group；Using product of the present invention
Afterwards, the egersis rate of mouse has increase, wherein and the egersis rate highest of 5 product group of embodiment, 7 days are 40%, and 14 days are 50%,
Egersis rate is above caffeine group.In addition, product of the embodiment of the present invention is smaller with the result difference of use 14 days using 7 days, say
Bright product of the present invention is after using 7 days, so that it may play its drug effect.Mouse yellow Jackets hypnosis experiment shows product of the present invention
It can inhibit pharmaceutical hypnosis effect, tool has the effect of refreshing.
Embodiment 8
Hide 1 product of schizonepeta compound essential oil embodiment, 2 product of embodiment, the antifatigue experiment of 5 product of embodiment
Sample: hiding 1 product of schizonepeta compound essential oil embodiment, 2 product of embodiment, 5 product of embodiment, positive control are coffee
Cause.
Experimentation and result
Administration mode: being put into Fragrance Lamp pallet in closed experiment box, successively extracts 10mL distilled water and 0.1mL compound essence
Oil is put into pallet.Fragrance Lamp is heated, after essential oil is spread uniformly in box, mouse is put into box, sucks essential oil,
Mouse is taken out after 30min, twice daily, continues 14.Caffeine carries out (dosage: 10mg/ by the way of gastric infusion
Kg), equally continue 14.
Take Kunming mouse 50,20 ± 2g of weight, half male and half female is randomly divided into 5 groups: 1 product group of embodiment, embodiment
2 product groups, 5 product group of embodiment, caffeine group, blank control group.It is 30 ± 2 that mouse, which is put into temperature, after last dose 1h
DEG C, the weight swimming of the swimming trunk load weight 5% of depth of water 40cm, record mouse starts swimming and sinks to water surface 8s to being perfectly exhausted
Time until cannot emerging, the time are mouse swimming time.Mouse is taken out after having recorded swimming time, is located
Extremely, liver and leg muscle are taken out immediately, prepares hepatic tissue and muscle homogenate, measure hepatic glycogen according to Anthrone-sulfuricacid method
And muscle glycogen content.It the results are shown in Table 5.
5 embodiment product of table to Tolerance of Swimming influence ()
Note: compared with blank control group, P < 0.01 * P < 0.05, * *；Compared with caffeine group,ΔP<0.05。
The product that refreshes the mind can be relieved human-body fatigue sense more, and therefore, the present invention uses mouse swimming test, multiple to hiding schizonepeta
Square essential oil product is evaluated.As seen from the results in Table 5, in mouse anti-reflecting fatigue experiment, three products of the invention and coffee are used
Cause can extend mouse swimming time, compared with blank control group, have extremely significant sex differernce (P < 0.01), wherein this hair
Bright three products are superior to caffeine to the extension of swimming time, and the swimming time of 5 product of embodiment is compared with caffeine group,
Time (P < 0.05) can significantly be extended；Meanwhile table 5 is the results show that three products of the invention and caffeine can be improved in liver
Hepatic glycogen content is horizontal, compared with blank control group, has extremely significant sex differernce (P < 0.01), meanwhile, the glycogen of three product groups
Raw water is averagely higher than caffeine, wherein 5 product of embodiment has significant difference (P < 0.05) compared with caffeine group；The present invention
Three products and caffeine can also increase the content of muscle glycogen, compared with blank control group, there is extremely significant sex differernce (P < 0.01),
And the muscle glycogen content of three product groups is improved compared with caffeine group, 5 groups of embodiment have significant difference (P <
0.05).It is above-mentioned the experiment results show that product of the embodiment of the present invention have it is apparent extend swimming time, increase hepatic glycogen
With the effect of muscle glycogen content.
Embodiment 9
It is small to behavioral despair depression model to hide 1 product of schizonepeta compound essential oil embodiment, 2 product of embodiment, 5 product of embodiment
The influence of mouse
It is hydrochloric acid that sample, which hides 1 product of schizonepeta compound essential oil embodiment, 2 product of embodiment, 5 product of embodiment, positive control,
Fluoxetine tablets.
Experimentation and result
Administration mode: being put into Fragrance Lamp pallet in closed experiment box, successively extracts 10mL distilled water and 0.1mL compound essence
Oil is put into pallet.Fragrance Lamp is heated, after essential oil is spread uniformly in box, rat is put into box, sucks essential oil,
Rat is taken out after 30min, it is twice daily, for 4 weeks.Fluoxetine tablet carried out by the way of gastric infusion (dosage:
3mg/kg), same for 4 weeks.
It male Kunming strain mice about 120, is randomly divided into 6 groups: being respectively 1 product group of embodiment, 2 product of embodiment
Group, 5 product group of embodiment, Fluoxetine hydrochloride group, model group, normal group, it is every group 20 average, in addition to normally organizing 5, every cage,
The remaining equal single cage raising of group.Model process continues 8 weeks, is given once daily a kind of stimulation of mouse, including fasting for 24 hours, prohibit water for 24 hours, folder
Tail 1min, it wet environment, overturns round the clock, outstanding tail 5min, ice water swimming (4 DEG C, 4min).Average every kind of stimulation has weekly one
Secondary, the stimulation sequence in each week is different, prevents mouse from which kind of stimulation predicted.From 4th week, start while to stimulation every
It is administered, and administration process continues surrounding.
Tail-suspention test: after last time administration 1h, by mouse tail end tip 1cm with immobilization with adhesive tape on iron, body
In projecting state, head distance ground about 50cm is around isolated with plastic plate, is interfered with excluding surrounding enviroment to it.Observation
Time continues 6 minutes, with the dead time of mouse in 4 minutes after stopwatch bulk registration, standard be mouse four limbs trunk completely not
It is dynamic.
Forced swim test: after last time administration 1h, mouse is put into high 20cm, diameter 15cm, depth of water 10cm, water temperature
To make it be forced swimming 6 minutes in 25 ± 1 DEG C of water drums, with the dead time of mouse in 4 minutes after stopwatch bulk registration, mark
Quasi-: mouse systemic only has head to expose the surface, and four limbs are motionless in floating or equal for the done mild action that makes oneself not being drowned
For motionless state.Above-mentioned experimental result is shown in Table 6.
6 embodiment product of table to desperate depression model mouse behavior influence (N=10)
Note: compared with model group, P < 0.01 * P < 0.05, * *.
Animal models of depression is to suffer animal the most commonly used is the long-term moderate Stress model of unpredictability, principle
Occur a series of emotion-directed behaviors after by long-term stress to change, if spontaneous activity is reduced, learning ability decline, appetite stimulator etc..By force
Urgent swimming test is also known as behavioral despair experiment, and animal is allowed to be forced to swim in water vat, and animal is initially risked one's life in water to move about, earns
Prick, attempt to escape, feel to escape therewith can not, just no longer struggle travelling, only head is exposed the surface, four limbs floating, maintain
The principle of a kind of motionless state, referred to as " behavioral despair " tail-suspention test is similar with its, for the angle of drug screening, both
Index has the pharmacology predictability validity of highest level, is widely used in the screening of antidepressants.
Above-mentioned despair depression model mouse test results show that three embodiment products of the invention can be shortened depression model
The mouse tail suspension dead time, wherein 5 product of embodiment has extremely significant sex differernce (P < 0.01), embodiment 1 compared with model group
With 2 product of embodiment compared with model group, there is significant difference (P < 0.05)；Three embodiment products can be shortened depression model
Mouse non-swimming time, wherein 5 product of embodiment has extremely significant sex differernce (P < 0.01), embodiment 1 compared with model group
With 2 product of embodiment compared with model group, there is significant difference (P < 0.05).The above results explanation, product of the present invention have one
Fixed antidepressant effect.
The above content is the further descriptions for combining specific preferred embodiment to be the present invention, are convenient for the skill
The technical staff in art field can understand and apply the invention, and it cannot be said that specific implementation of the invention is only limited to these instructions.
For those of ordinary skill in the art to which the present invention belongs, it can also make without departing from the inventive concept of the premise
Several simple deduction or replace, without having to go through creative labor.Therefore, those skilled in the art are according to the present invention takes off
Show, the simple modifications made to the present invention all should be within protection scope of the present invention.
Claims (3)
1. a kind of hiding schizonepeta compound essential oil, by weight, by Herba Schizonepetae volatile oil 30-50 parts of hiding, rhododendron anthopogonoide volatile oil 10-
20 parts, 10-20 parts of Carum carvi volatile oil, 10-20 parts of Herba Dracocephali Tangutici volatile oil, 10-20 parts of hyssop volatile oil compositions；
The preparation method that Herba Schizonepetae volatile oil is hidden in the essential oil is: after taking hiding schizonepeta herb to be ground into coarse powder, 12 times of amounts are added
Water impregnates 1h, extracts 6h using steam distillation method, and in oil water separator after 2~3h of settle and separate, collection is hidden
Herba Schizonepetae volatile oil；
The preparation method of the rhododendron anthopogonoide volatile oil is: taking rhododendron anthopogonoide and leaf, after being ground into coarse powder, is added 10 times
Water is measured, 1h is impregnated, 8h is extracted using steam distillation method, in oil water separator after 2~3h of settle and separate, collection is obtained
Rhododendron anthopogonoide volatile oil；
The preparation method of the Carum carvi volatile oil is: after taking Carum carvi fruit to be ground into coarse powder, 8 times of amount water are added, impregnate
1h extracts 4h using steam distillation method, and in oil water separator after 2~3h of settle and separate, collection obtains Carum carvi volatilization
Oil；
The preparation method of the Herba Dracocephali Tangutici volatile oil is: after taking Herba Dracocephali Tangutici herb to be ground into coarse powder, 10 times of amount water are added,
2h is impregnated, 8h is extracted using steam distillation method, in oil water separator after 2~3h of settle and separate, collection obtains sweet blue and green
Blue volatile oil；
The preparation method of the hyssop volatile oil is: after taking hyssop herb to be ground into coarse powder, 8 times of amount water are added, impregnate
2h extracts 6h using steam distillation method, and in oil water separator after 2~3h of settle and separate, collection obtains hyssop volatilization
Oil；
Administration mode: successively extracting 10mL distilled water and 0.1 mL compound essential oil and be put into Fragrance Lamp pallet, by Fragrance Lamp plus
Heat sucks essential oil after essential oil diffusion uniformly.
2. a kind of preparation method of hiding schizonepeta compound essential oil according to claim 1, it is characterised in that: by hiding schizonepeta volatilization
Oil, rhododendron anthopogonoide volatile oil, Carum carvi volatile oil, Herba Dracocephali Tangutici volatile oil, hyssop volatile oil is according to claim 1 matches
Than getting all the ready, it is sequentially placed into stirring container, whipping temp is 20~25 DEG C, 80~100r/min of mixing speed, equidirectional stirring
30~60min is sufficiently mixed each raw material uniformly, and packing seals to get finished product is arrived.
3. hiding schizonepeta compound essential oil according to claim 1 is depressured, refreshed the mind in preparation treatment, antifatigue and anti-suppression
Application in strongly fragrant drug.
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1306857A (en) * | 1999-05-25 | 2001-08-08 | 青海省老年医学研究所 | Medicine for strengthening physique and strengthening immunity |
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2015
- 2015-11-19 CN CN201510800115.3A patent/CN105287723B/en active Active
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1306857A (en) * | 1999-05-25 | 2001-08-08 | 青海省老年医学研究所 | Medicine for strengthening physique and strengthening immunity |
Non-Patent Citations (2)
| Title |
|---|
| 甘青青兰挥发油化学成分研究;黄小平等;《成都中医药大学学报》;20070630;第30卷(第2期);第61页右栏第2段 * |
| 青岛科技大学学报(自然科学版);公衍玲等;《青岛科技大学学报(自然科学版)》;20140630;第35卷(第3期);第264页左栏倒数第2段至右栏倒数第1段 * |
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