CN116162524A - Production device for trichoderma fermentation - Google Patents

Abstract

The invention belongs to the technical field of microbial cultivation, in particular to a production device for trichoderma fermentation, which aims at solving the problem that the phenomenon of deposition of culture solution can occur due to the fact that trichoderma is positioned on the surface of the culture solution in the subsequent fermentation and cultivation process, so that the bottom culture solution can not be absorbed, and the cultivation effect of trichoderma is reduced. The production device for trichoderma fermentation disclosed by the invention can realize the intermittent quantitative liquid adding operation of trichoderma culture in the fermentation kettle body, improve the high efficiency of the layered culture process of trichoderma, avoid the influence of the deposition of culture solution caused by excessive addition of culture solution in one time in the culture process, influence the absorption effect of trichoderma on the culture solution, promote the formation of a plurality of layers of trichoderma by using the intermittent quantitative liquid adding mode, and improve the overall culture efficiency of trichoderma.

Description

Production device for trichoderma fermentation

Technical Field

The invention relates to the technical field of microbial cultivation, in particular to a production device for trichoderma fermentation.

Background

Trichoderma ascomycetes, hypocreaceae, trichoderma, are widely present in soil under different environmental conditions, most Trichoderma can produce various bioactive substances having antagonism to plant pathogenic fungi, bacteria and insects, such as cell wall degrading enzymes and secondary metabolites, and can improve stress resistance of crops, promote plant growth and increase yield of agricultural products, and thus are widely used for biological control, biofertilizer and soil conditioner.

The existing trichoderma fermentation culture process is to directly add bacterial colonies and culture solution into a culture reaction kettle for carrying out, and as trichoderma is positioned on the surface of the culture solution in the subsequent fermentation culture process, the phenomenon of deposition of the culture solution can be caused, so that the culture solution at the bottom can not be absorbed, and the culture effect of trichoderma is reduced.

Disclosure of Invention

The invention discloses a production device for trichoderma fermentation, and aims to solve the technical problems that a culture solution is deposited and can not be absorbed in the background art, and the culture effect of trichoderma is reduced.

The invention provides a production device for trichoderma fermentation, which comprises a fermentation kettle body, wherein the fermentation kettle body comprises an outer cylinder, an inner cylinder and a bottom cylinder, the bottom cylinder is positioned below the outer cylinder and the inner cylinder, the outer cylinder and the inner cylinder are fixedly connected with the bottom cylinder, arc-shaped holes are formed in the outer cylinder and the inner cylinder, the inner part of each arc-shaped hole is fixedly connected with the same observation bin, toughened glass is arranged on each observation bin, three culture plates which are equidistant up and down are arranged in the inner cylinder, a liquid chamber is arranged in the bottom cylinder, a culture liquid returning component is arranged above the liquid chamber, the culture liquid returning component comprises a liquid returning pipe and a liquid feeding pipe, the liquid returning pipe is positioned in the inner cylinder, the lower end of the liquid returning pipe is fixedly connected with a liquid sucking bin, the liquid feeding pipe is fixedly connected with the outer wall of the inner cylinder, three liquid feeding bins which are equidistant up and down are positioned in the inner cylinder, and three liquid feeding bins which are positioned above the culture plates obliquely.

Through being provided with the culture solution and returning the subassembly, utilize the culture solution to return the subassembly and can realize the interval formula ration liquid feeding operation that this internal trichoderma of fermentation vat cultivateed, improve the high efficiency of trichoderma layering culture process, avoid adding the culture solution too much because of once in the culture process and cause the culture solution deposit, influence trichoderma to the absorption effect of culture solution, utilize interval formula ration liquid feeding mode can promote trichoderma to form a plurality of layering, improve trichoderma's whole cultivation efficiency.

In a preferred scheme, a cavity is arranged between the outer cylinder and the inner cylinder, water is filled in the cavity, and a water bath temperature control assembly is arranged in the cavity; the water bath temperature control assembly comprises a water adding pipe, a drain pipe and a plurality of heating rods, wherein the water adding pipe and the drain pipe are fixedly connected with the cavities of the outer cylinder and the inner cylinder, the water adding pipe is located above the drain pipe, sealing covers are arranged on the water adding pipe and the drain pipe, the heating rods are connected with the bottom cylinder through bolts and bolts, and a plurality of contacts which are equidistant up and down are arranged on the heating rods.

The water bath temperature control assembly is arranged, so that the temperature of the trichoderma fermenting environment can be controlled in real time by utilizing the water temperature in the cavities of the outer cylinder and the inner cylinder, the trichoderma fermenting process is ensured to be under the optimal temperature condition, and the fermentation of trichoderma is improved; the contact effect between the heating rod and the water can be increased through the contacts on the heating rod, so that the temperature of the water in the cavity can be quickly regulated and controlled when the heating rod is required, and the overall temperature control effect is improved.

In a preferred scheme, the top fixedly connected with cauldron lid of fermentation kettle body, the top fixedly connected with motor of cauldron lid, the output of motor has the main shaft through the coupling joint, has seted up the round hole on the cauldron lid, and the round hole fixedly connected with axostylus axostyle that the lower extreme of main shaft passed the cauldron lid, and all offered the slot that runs through on the three cultivation dish, and the three cultivation dish all is located the outside of axostylus axostyle, all is provided with the locking position and inserts between three cultivation dish and the axostylus axostyle.

Through being provided with axostylus axostyle and locking position and inserting, adopt the mode of locking position to insert between three cultivation dish and the axostylus axostyle to fix, the installation of the cultivation dish of being convenient for and follow-up transfer after accomplishing the cultivation.

In a preferred scheme, three flow guide components which are equidistant up and down are arranged in the inner cylinder, and the three flow guide components are positioned on one side of the culture disc far away from the liquid return pipe; the guide assembly comprises a guide plate and a plurality of mixing rods, the guide plate is located below the liquid returning bin, the liquid guiding groove is formed in the upper side of the guide plate and is in inclined arrangement, liquid falling holes are formed in the lower end of the liquid guiding groove, the liquid falling holes are formed in the upper side of the culture disc, two rows of equidistant circular caulking grooves are formed in the lower side of the guide plate, the two rows of circular caulking grooves are symmetrically distributed, one ends of the mixing rods are movably connected with the inner portions of the circular caulking grooves, the other ends of the mixing rods are fixedly connected with spherical weighting heads, round holes are formed in the guide plate, rotary rods are fixedly connected to the inner portions of the round holes, and the two ends of each rotary rod are movably connected with the inner barrel.

Through being provided with the water conservancy diversion subassembly, utilize the water conservancy diversion subassembly can assist the continuity of entering culture dish after the discharge of liquid feed bin, avoid sending the excessive drop between liquid feed bin and the culture dish to cause the culture fluid to splash, a plurality of mixing bars on the guide plate can utilize the reaction force that contacts with the culture fluid on the culture dish to change its motion state to mix stirring to culture fluid and trichoderma, improve trichoderma to the absorption of culture fluid, supplementary trichoderma dispersion on the culture dish.

In a preferred scheme, a plurality of the section of thick bamboo wall fixedly connected with turbine that the one end of bull stick passed the inner tube, and the mounting groove has been seted up to the outside of inner tube, and the inside fixedly connected with first reverse motor of mounting groove, the output of first reverse motor has the pivot through the coupling joint, swing joint between the other end of pivot and the mounting groove inner wall of inner tube is provided with the worm of three equidistance from top to bottom in the pivot, and three worm passes through the tooth's socket meshing with three turbine respectively.

The first reversing motor, the rotating shaft and the worm are arranged, the first reversing motor drives the rotating shaft to rotate, the worm is meshed with the turbine, the rotating rod drives the guide plate to overturn, and the mixing rod is sagged under the action of gravity to be stored; the first reversing motor is utilized to drive the guide plate to overturn, so that the guide assembly is accommodated, and the placing of the culture plate and the transfer after the subsequent completion of fermentation culture are facilitated.

In a preferred scheme, the inside fixedly connected with functional box of kettle cover, the inside of functional box is provided with divides liquid temperature regulating assembly, and divides liquid temperature regulating assembly to include transfer line and temperature regulating piece, the both ends of transfer line are provided with pipette and fluid-discharge tube respectively, swing joint between pipette and the return liquid pipe, swing joint between fluid-discharge tube and the liquid supply pipe is provided with miniature liquid pump on the transfer line, miniature liquid pump is located between pipette and the fluid-discharge tube, the temperature regulating piece is located the top of transfer line, be provided with a plurality of contact plates that are circumference equidistance on the temperature regulating piece, contact between a plurality of contact plates all and the transfer line.

Through being provided with branch liquid temperature regulating subassembly, utilize branch liquid temperature regulating subassembly can carry out the intensification to the culture solution and handle at the culture solution from the time of liquid return pipe to liquid feed pipe transport, make the temperature of culture solution slightly higher than the whole temperature in the inner tube (in the culture process, form intensive hypha after trichoderma fermentation in the culture dish to having hindered external temperature's transmission effect, the culture solution temperature of follow-up joining is slightly higher, can improve the whole temperature of culture solution in the culture dish, thereby guarantee to ferment and cultivate in the in-process and be in the optimal temperature all the time.

In a preferred scheme, the inside of inner tube is provided with spiral oxygen therapy pipe, and spiral oxygen therapy pipe is located the outside of three cultivation dish, fixed connection between the inner wall of spiral oxygen therapy pipe and inner tube, and one side that is close to the cultivation dish on the spiral oxygen therapy pipe all is provided with the oxygen groove, and the round hole has been seted up on the cauldron lid, and the inside fixedly connected with of round hole send the oxygen pipe, send swing joint between the inner port of oxygen pipe and the spiral oxygen therapy pipe, send through flange joint between the outer port of oxygen pipe and the outside oxygen pipeline.

Through being provided with spiral oxygen therapy pipe, utilize the spiral oxygen therapy pipe can utilize the mode of screw transportation to make inside oxygen form the heliciform to guarantee the even of oxygen distribution in the inner tube, the heliciform oxygen that flows simultaneously can further improve the contact between oxygen and the trichoderma, improves trichoderma's absorption effect.

In a preferred scheme, the upside fixedly connected with connecting seat of cover, fixedly connected with crane on the connecting seat, the crane is located the outside of fermentation vat body, one side of keeping away from the fermentation vat body on the crane is provided with back of the body pinion rack, one side of fermentation vat body is provided with the portal frame, the spout has been seted up on the portal frame, swing joint between crane and the portal frame, and fixedly connected with motor support on the portal frame, fixedly connected with second reverse motor on the motor support, the output of second reverse motor passes through the coupling joint and has the minor axis, the outside fixedly connected with lifting gear of minor axis, through the tooth's socket meshing between lifting gear and the back of the body pinion rack.

Therefore, the production device for trichoderma fermentation provided by the invention has the advantages that the intermittent quantitative liquid adding operation of trichoderma culture in the fermentation kettle body can be realized, the high efficiency of the layered culture process of trichoderma is improved, the phenomenon that the absorption effect of trichoderma on the culture solution is affected due to the fact that too much culture solution is added once in the culture process is avoided, the trichoderma can be promoted to form a plurality of layers by utilizing the intermittent quantitative liquid adding mode, and the overall culture efficiency of the trichoderma is improved.

Drawings

FIG. 1 is a schematic diagram of a fermentation kettle body of a production device for fermenting trichoderma;

FIG. 2 is a schematic diagram of the outer cylinder structure of the production device for trichoderma fermentation;

FIG. 3 is a schematic diagram of a water bath temperature control assembly of a production device for trichoderma fermentation;

FIG. 4 is a schematic diagram of a production device for fermenting Trichoderma reesei;

FIG. 5 is a schematic diagram of a flow guiding assembly of a production device for fermenting Trichoderma;

FIG. 6 is a schematic diagram showing the cross-sectional structure of a kettle cover of a production device for fermenting trichoderma;

FIG. 7 is a schematic diagram of the functional box structure of the production device for fermenting trichoderma;

FIG. 8 is a schematic diagram of the overall structure of a production device for fermenting trichoderma;

fig. 9 is a schematic view of a portal frame structure of a production device for trichoderma fermentation.

In the figure: 1. a fermentation tank body; 2. an outer cylinder; 3. an inner cylinder; 4. a bottom cylinder; 5. an observation bin; 6. a liquid chamber; 7. a culture fluid return assembly; 701. a liquid return pipe; 702. a liquid feeding pipe; 703. a liquid suction bin; 704. a liquid feeding bin; 8. a culture tray; 9. a water bath temperature control assembly; 901. a water supply pipe; 902. a drain pipe; 903. a heating rod; 904. a cover; 905. a contact; 10. a kettle cover; 11. a motor; 12. a main shaft; 13. a shaft lever; 14. locking position insertion; 15. a flow guiding assembly; 1501. a deflector; 1502. a mixing rod; 1503. a liquid guiding groove; 1504. a liquid falling hole; 1505. a spherical weighting head; 1506. a rotating rod; 16. a turbine; 17. a first reversing motor; 18. a rotating shaft; 19. a worm; 20. a functional box; 21. a liquid separation temperature regulating component; 2101. an infusion tube; 2102. a temperature regulating sheet; 2103. a pipette; 2104. a liquid discharge pipe; 2105. a miniature liquid pump; 22. a spiral oxygen therapy tube; 23. an oxygen discharge tank; 24. an oxygen supply pipe; 25. a connecting seat; 26. a lifting frame; 27. a back toothed plate; 28. a portal frame; 29. a motor support; 30. a second reversing motor; 31. lifting the gear.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments.

The invention discloses a production device for trichoderma fermentation, which is mainly applied to the scene of low trichoderma fermentation culture efficiency.

Referring to fig. 1-4, a trichoderma fermenting production device, including fermentation kettle body 1, fermentation kettle body 1 includes urceolus 2, inner tube 3 and end section of thick bamboo 4, and end section of thick bamboo 4 is located the below of urceolus 2 and inner tube 3, pass through bolted connection between urceolus 2 and inner tube 3 and the end section of thick bamboo 4, the arc hole has been seted up on urceolus 2 and the inner tube 3, the inside in arc hole has same observation storehouse 5 through bolted connection, and be provided with toughened glass on the observation storehouse 5, the inside of inner tube 3 is provided with the culture dish 8 of three equidistance about, and the inside of end section of thick bamboo 4 is provided with liquid chamber 6, the top of liquid chamber 6 is provided with culture solution and returns subassembly 7, culture solution returns subassembly 7 and includes liquid returning pipe 701 and liquid delivering pipe 702, and liquid returning pipe 701 is located the inside of inner tube 3, the lower extreme through bolted connection of liquid returning pipe 701 has liquid suction storehouse 703, liquid suction storehouse 703 is located liquid chamber 6, and liquid delivering pipe 702 and the outer wall through bolted connection, and liquid delivering pipe 702 is last to be provided with liquid delivering storehouse 704 of three inner tube, three equidistance about, three liquid delivering storehouse 704 is located three liquid delivering storehouse 3 respectively and is located three and is 8 on the culture dish 8.

Specifically, the culture bacteria are placed in three culture trays 8, the culture solution is injected into the solution chamber 6, the fermentation kettle body 1 is sealed, in the culture process, the solution returning pipe 701 sucks the culture solution in the solution chamber 6 into the solution returning pipe 701 through the solution sucking bin 703 and conveys the culture solution into the solution conveying pipe 702, the quantitative culture solution is conveyed into the culture trays 8 by utilizing the liquid level difference in the solution conveying pipe 702 and sequentially passing through the solution conveying bin 704, the trichoderma in the culture trays 8 is fermented and cultured, and after a certain time, the operation is repeated, and the conveying of the culture solution to the culture trays 8 is continued;

in specific application scenario, culture solution returns subassembly 7 is applicable to the ration liquid feeding link of trichoderma in-process of cultivateing, utilizes culture solution to return subassembly 7 promptly and can realize the spaced ration liquid feeding operation of trichoderma cultivation in the fermentation vat body 1, improves the high efficiency of trichoderma layering cultivate process, avoids adding the excessive culture solution because of once in the cultivate process and causes the culture solution deposit, influences trichoderma to the absorbing effect of culture solution, utilizes spaced ration liquid feeding's mode can promote trichoderma to form a plurality of layering, improves trichoderma's whole cultivation efficiency.

Referring to fig. 1, 2 and 3, a cavity is arranged between the outer cylinder 2 and the inner cylinder 3, water is filled in the cavity, and a water bath temperature control assembly 9 is arranged in the cavity; the water bath temperature control assembly 9 comprises a water adding pipe 901, a water draining pipe 902 and a plurality of heating rods 903, wherein the water adding pipe 901 and the water draining pipe 902 are connected with the cavities of the outer cylinder 2 and the inner cylinder 3 through bolts, the water adding pipe 901 is located above the water draining pipe 902, sealing covers 904 are arranged on the water adding pipe 901 and the water draining pipe 902, the heating rods 903 are connected with the bottom cylinder 4 through bolts, and a plurality of contacts 905 which are equidistant from top to bottom are arranged on the heating rods 903.

Specifically, water required for temperature control is added into the cavities of the outer cylinder 2 and the inner cylinder 3 through the water adding pipe 901, and the water in the cavities is heated by the heating rod 903 in the culture process, so that the temperature of the inner cylinder 3 is kept constant, and the optimal temperature for trichoderma culture is ensured through heat conduction;

in a specific application scene, the water bath temperature control assembly 9 is suitable for a temperature control and adjustment link of the temperature of the inner cylinder 3 in the trichoderma fermentation process, namely, the water bath temperature control assembly 9 can be used for controlling the temperature of the trichoderma fermentation environment in real time by utilizing the water temperature in the cavities of the outer cylinder 2 and the inner cylinder 3, so that the trichoderma fermentation process is ensured to be under the optimal temperature condition, and the trichoderma fermentation is improved; the contact effect between the heating rod 903 and water can be increased by the contacts 905 on the heating rod 903, so that the temperature of water in the cavity can be quickly regulated and controlled when the water is required, and the overall temperature control effect is improved.

Referring to fig. 1, 4 and 6, the upper side of the fermentation kettle body 1 is connected with a kettle cover 10 through a bolt, the upper side of the kettle cover 10 is connected with a motor 11 through a bolt, the output end of the motor 11 is connected with a main shaft 12 through a coupling, a round hole is formed in the kettle cover 10, the lower end of the main shaft 12 penetrates through the round hole of the kettle cover 10 and is connected with a shaft lever 13 through a bolt, through slots are formed in three culture plates 8, the three culture plates 8 are located outside the shaft lever 13, and locking inserts 14 are arranged between the three culture plates 8 and the shaft lever 13.

Specifically, during the culturing process, the motor 11 drives the shaft lever 13 to rotate through the main shaft 12, so that the three culturing trays 8 are in a uniform and slow rotating state along with the shaft lever 13; the three culture trays 8 and the shaft rod 13 are fixed by adopting a locking insert 14, so that the culture trays 8 are convenient to install and transfer after the subsequent culture is finished.

Referring to fig. 1, 4 and 5, three flow guide assemblies 15 which are equidistant up and down are arranged inside the inner cylinder 3, and the three flow guide assemblies 15 are all positioned on one side of the culture dish 8 far away from the liquid return pipe 701; the water conservancy diversion subassembly 15 includes guide plate 1501 and a plurality of miscella 1502, the guide plate 1501 is located the below in back liquid storehouse, guide groove 1503 has been seted up to the upside of guide plate 1501, guide groove 1503 is the slope setting, the liquid hole 1504 has been seted up to the lower extreme of guide groove 1503, liquid hole 1504 is located the top of culture dish 8, two rows of equidistant circular caulking grooves have been seted up to the downside of guide plate 1501, two rows of circular caulking grooves are the symmetric distribution, the one end of a plurality of miscella 1502 is rotated with the inside of a plurality of circular caulking grooves and is connected, the other end of a plurality of miscella 1502 has spherical weighting head 1505 through bolted connection, and the round hole has been seted up on the guide plate 1501, the inside of round hole has the bull stick 1506 through bolted connection, all pass through the bearing rotation between the both ends and the inner tube 3 of 1506 and be connected.

Specifically, the culture solution is discharged from the solution feeding bin 704, falls onto the guide plate 1501, slides along the solution flowing groove, and enters the culture dish 8 from the solution falling hole 1504, in the whole culture process, the three culture dishes 8 are in a slow uniform rotation state, the mixing rod 1502 on the guide plate 1501 is driven by the reaction force of the culture solution on the culture dish 8, so that the mixing rod 1502 is in an irregular shaking state to mix and stir the culture solution in the culture dish 8 and trichoderma, the absorption of the trichoderma to the culture solution is improved, and meanwhile, the trichoderma is assisted to be dispersed more uniformly on the culture dish 8;

in a specific application scenario, the flow guiding component 15 is suitable for a flow guiding link that the culture solution enters the culture dish 8, namely, the flow guiding component 15 is utilized to assist the continuity that the culture solution enters the culture dish 8 after being discharged from the liquid feeding bin 704, so that the drop between the liquid feeding bin 704 and the culture dish 8 is avoided to excessively cause the splashing of the culture solution, and meanwhile, the plurality of mixing rods 1502 on the flow guiding plate 1501 can change the motion state of the flow guiding component by utilizing the reaction force contacted with the culture solution on the culture dish 8, so that the culture solution and trichoderma are mixed and stirred, the absorption of the trichoderma to the culture solution is improved, and the dispersion of the trichoderma on the culture dish 8 is assisted.

Referring to fig. 4 and 5, one end of a plurality of rotating rods 1506 passes through the wall of the inner cylinder 3 and is connected with a turbine 16 through a bolt, and the outer part of the inner cylinder 3 is provided with a mounting groove, the inner part of the mounting groove is connected with a first reversing motor 17 through a bolt, the output end of the first reversing motor 17 is connected with a rotating shaft 18 through a coupling, the other end of the rotating shaft 18 is rotatably connected with the inner wall of the mounting groove of the inner cylinder 3 through a bearing, three upper and lower equidistant worms 19 are arranged on the rotating shaft 18, and the three worms 19 are respectively meshed with the three turbines 16 through tooth grooves.

Specifically, the first reversing motor 17 drives the rotating shaft 18 to rotate, the worm 19 is meshed with the turbine 16, the rotating rod 1506 drives the flow guide plate 1501 to turn over, and the mixing rod 1502 is sagged under the action of gravity to be stored; the first reversing motor 17 is utilized to drive the flow guide plate 1501 to turn over, so that the flow guide assembly 15 is accommodated, and the placement of the culture dish 8 and the transfer after the subsequent fermentation culture are facilitated.

Referring to fig. 6 and 7, the functional box 20 is connected to the inside of the kettle cover 10 through bolts, the liquid-separating temperature-regulating assembly 21 is arranged in the functional box 20, the liquid-separating temperature-regulating assembly 21 comprises a liquid-conveying pipe 2101 and a temperature-regulating plate 2102, a liquid-sucking pipe 2103 and a liquid-discharging pipe 2104 are respectively arranged at two ends of the liquid-conveying pipe 2101, the liquid-sucking pipe 2103 is connected with the liquid-returning pipe 701 in an inserting mode, the liquid-discharging pipe 2104 is connected with the liquid-conveying pipe 702 in an inserting mode, a miniature liquid pump 2105 is arranged on the liquid-conveying pipe 2101, the miniature liquid pump 2105 is arranged between the liquid-sucking pipe 2103 and the liquid-discharging pipe 2104, the temperature-regulating plate 2102 is arranged above the liquid-conveying pipe 2101, and a plurality of contact plates with equal circumferencial distances are arranged on the temperature-regulating plate 2102, and the contact plates are contacted with the liquid-conveying pipe 2101.

Specifically, the culture fluid flows from the infusion tube 2101 (the micro liquid pump 2105 is used for providing power to complete the transfer of the culture fluid from the liquid return tube 701 to the liquid delivery tube 702) during the period of transferring the culture fluid from the liquid return tube 701 to the liquid delivery tube 702, and the temperature regulating plate 2102 heats the culture fluid in the infusion tube 2101 to raise the temperature thereof;

in a specific application scenario, the liquid-separating and temperature-regulating assembly 21 is suitable for a temperature-regulating link of the culture solution during the period of conveying the culture solution from the liquid return pipe 701 to the liquid conveying pipe 702, namely, the liquid-separating and temperature-regulating assembly 21 is utilized to heat the culture solution during the period of conveying the culture solution from the liquid return pipe 701 to the liquid conveying pipe 702, so that the temperature of the culture solution is slightly higher than the whole temperature in the inner barrel 3 (the trichoderma in the culture tray 8 is fermented to form dense hyphae in the culture process, thereby preventing the transmission effect of the external temperature, the temperature of the culture solution added later is slightly higher, and the whole temperature of the culture solution in the culture tray 8 can be improved, so that the optimal temperature is always maintained in the fermentation culture process.

Referring to fig. 1 and 6, a spiral oxygen therapy tube 22 is arranged inside the inner cylinder 3, the spiral oxygen therapy tube 22 is positioned outside the three culture plates 8, the spiral oxygen therapy tube 22 is connected with the inner wall of the inner cylinder 3 through bolts, an oxygen discharge groove 23 is formed in one side, close to the culture plates 8, of the spiral oxygen therapy tube 22, a round hole is formed in the kettle cover 10, an oxygen therapy tube 24 is connected inside the round hole through bolts, an inner port of the oxygen therapy tube 24 is inserted between the spiral oxygen therapy tube 22, and an outer port of the oxygen therapy tube 24 is connected with an external oxygen transmission pipeline through a flange.

Specifically, the oxygen demand of trichoderma in the fermentation culture process is large, the external oxygenerator is used for conveying oxygen into the oxygen conveying pipe 24 through the external oxygen conveying pipeline, and then the oxygen is uniformly conveyed to the inner barrel 3 through the spiral oxygen conveying pipe 22, so that the oxygen content in the inner barrel 3 is ensured to meet the demand; the spiral oxygen therapy tube 22 can be used for enabling internal oxygen to form a spiral shape in a spiral conveying mode, so that the uniformity of oxygen distribution in the inner cylinder 3 is guaranteed, meanwhile, the flowing spiral oxygen can further improve the contact between the oxygen and trichoderma, and the absorption effect of the trichoderma is improved.

Referring to fig. 6, 8 and 9, the upside of the tank cover 10 is connected with a connecting seat 25 through a bolt, a lifting frame 26 is connected with the connecting seat 25 through a bolt, the lifting frame 26 is located on the outer side of the fermentation tank body 1, one side, far away from the fermentation tank body 1, of the lifting frame 26 is provided with a back toothed plate 27, one side of the fermentation tank body 1 is provided with a portal frame 28, a chute is formed in the portal frame 28, the lifting frame 26 is connected with the portal frame 28 in a sliding manner, a motor support 29 is connected with the portal frame 28 through a bolt, a second reversing motor 30 is connected with the motor support 29 through a bolt, the output end of the second reversing motor 30 is connected with a short shaft through a coupler, a lifting gear 31 is connected with the outer side of the short shaft through a bolt, and the lifting gear 31 is meshed with the back toothed plate 27 through a toothed groove.

Specifically, when the culture dish 8 is put into the fermentation kettle body 1 and the fermentation is carried out and then the culture dish 8 is transferred, the lifting gear 31 is driven by the second reversing motor 30 to engage with the back toothed plate 27, and the kettle cover 10 is driven by the lifting frame 26 to lift.

Working principle: when the trichoderma reesei culturing device is used, culturing bacteria are placed in three culturing trays 8, a culture solution is injected into a solution chamber 6, a fermentation kettle body 1 is sealed, water required for temperature control is added into cavities of an outer cylinder 2 and an inner cylinder 3 through a water adding pipe 901, and the water in the cavities is heated by a heating rod 903 in the culturing process, so that the temperature of the inner cylinder 3 is kept constant, and the most suitable temperature for trichoderma reesei culturing is ensured through heat conduction;

in the culturing process, the motor 11 drives the shaft rod 13 to rotate through the main shaft 12, so that the three culture trays 8 rotate slowly along with the shaft rod 13 at a constant speed, the liquid return pipe 701 sucks the culture liquid in the liquid chamber 6 into the liquid return pipe 701 through the liquid suction bin 703 and conveys the culture liquid into the liquid conveying pipe 702, the culture liquid flows from the liquid conveying pipe 2101 (the power is provided by the miniature liquid pump 2105 to finish the conveying of the culture liquid from the liquid return pipe 701 to the liquid conveying pipe 702) during the conveying of the culture liquid from the liquid return pipe 701 to the liquid conveying pipe 702, the temperature regulating plate 2102 heats the culture liquid in the liquid conveying pipe 2101 to raise the temperature, and then the liquid level difference in the liquid conveying pipe 702 is utilized to sequentially convey the quantitative culture liquid into the culture trays 8 through the liquid conveying bin 704 (the culture liquid is discharged from the liquid conveying bin and falls onto the guide plate 1501, the trichoderma is slid down along the liquid flowing groove and enters the culture dish 8 from the liquid falling hole 1504, in the whole culture process, the three culture dishes 8 are in a slow uniform rotation state, the mixing rod 1502 on the guide plate 1501 can be driven by the reaction force of the culture liquid on the culture dish 8, so that the mixing rod 1502 is in an irregular shaking state to mix and stir the culture liquid in the culture dish 8 and trichoderma, the absorption of the trichoderma to the culture liquid is improved, meanwhile, the trichoderma is assisted to be dispersed on the culture dish 8 more uniformly), the trichoderma in the culture dish 8 is fermented and cultured, and after a certain time, the operation is repeated, and the culture liquid is continuously conveyed to the culture dish 8.

The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.

Claims (8)

1. The utility model provides a trichoderma fermenting production device, includes fermentation kettle body (1), its characterized in that, fermentation kettle body (1) includes urceolus (2), inner tube (3) and end section of thick bamboo (4), and end section of thick bamboo (4) are located the below of urceolus (2) and inner tube (3), fixed connection between urceolus (2) and inner tube (3) and end section of thick bamboo (4), set up the arc hole on urceolus (2) and inner tube (3), the inside fixedly connected with in arc hole is same observes storehouse (5), and is provided with toughened glass on observing storehouse (5), the inside of inner tube (3) is provided with culture dish (8) of three equidistance from top to bottom, and the inside of end section of thick bamboo (4) is provided with liquid cavity (6), and the top of liquid cavity (6) is provided with culture solution and returns subassembly (7), culture solution returns subassembly (7) including return tube (701) and liquid feed-back tube (702), and return tube (702) are located the inside of inner tube (3), and liquid return tube (701) lower extreme fixedly connected with storehouse (703) and liquid suction storehouse (703) are located three liquid feed-back tube (702) and three liquid feed-through (704) are located inside (704) and three liquid feed-through (704) and are located inside three liquid feed-through (702) and are located inside (3) and three liquid feed-through (702, the three liquid feeding bins (704) are respectively positioned obliquely above the three culture trays (8);

a cavity is formed between the outer cylinder (2) and the inner cylinder (3), water is filled in the cavity, and a water bath temperature control assembly (9) is arranged in the cavity; the water bath temperature control assembly (9) comprises a water adding pipe (901), a drain pipe (902) and a plurality of heating rods (903), wherein the water adding pipe (901) and the drain pipe (902) are fixedly connected with the cavities of the outer barrel (2) and the inner barrel (3), the water adding pipe (901) is located above the drain pipe (902), sealing covers (904) are arranged on the water adding pipe (901) and the drain pipe (902), the heating rods (903) are connected with the bottom barrel (4) through bolts, and a plurality of contacts (905) which are equidistant from top to bottom are arranged on the heating rods (903).

2. The production device for trichoderma fermentation according to claim 1, wherein the upper part of the fermentation kettle body (1) is fixedly connected with a kettle cover (10), the upper part of the kettle cover (10) is fixedly connected with a motor (11), the output end of the motor (11) is connected with a main shaft (12) through a coupling, a round hole is formed in the kettle cover (10), the lower end of the main shaft (12) penetrates through the round hole of the kettle cover (10) and is fixedly connected with a shaft lever (13), through slots are formed in three culture plates (8), the three culture plates (8) are located outside the shaft lever (13), and locking inserts (14) are arranged between the three culture plates (8) and the shaft lever (13).

3. The production device for trichoderma fermentation according to claim 2, wherein three flow guiding components (15) are arranged in the inner cylinder (3) at equal intervals up and down, and the three flow guiding components (15) are all positioned on one side of the culture dish (8) far away from the liquid return pipe (701).

4. The production device for trichoderma fermentation according to claim 3, wherein the flow guiding component (15) comprises a flow guiding plate (1501) and a plurality of mixing rods (1502), the flow guiding plate (1501) is located below the liquid returning bin, the upper side of the flow guiding plate (1501) is provided with a liquid guiding groove (1503), the liquid guiding groove (1503) is obliquely arranged, the lower end of the liquid guiding groove (1503) is provided with a liquid dropping hole (1504), the liquid dropping hole (1504) is located above the culture disc (8), the lower side of the flow guiding plate (1501) is provided with two rows of equidistant circular caulking grooves, the two rows of circular caulking grooves are symmetrically distributed, one end of the plurality of mixing rods (1502) is movably connected with the inner portion of the plurality of circular caulking grooves, the other end of the plurality of mixing rods (1502) is fixedly connected with a spherical weighting head (1505), the flow guiding plate (1501) is provided with a round hole, the round hole is fixedly connected with a rotary rod (1506), and two ends of the rotary rod (1506) are movably connected with the inner cylinder (3).

5. The production device for trichoderma fermentation according to claim 4, wherein one end of the rotating rods (1506) penetrates through the wall of the inner cylinder (3) and is fixedly connected with the turbine (16), a mounting groove is formed in the outer portion of the inner cylinder (3), a first reversing motor (17) is fixedly connected to the inner portion of the mounting groove, a rotating shaft (18) is connected to the output end of the first reversing motor (17) through a coupling, the other end of the rotating shaft (18) is movably connected with the inner wall of the mounting groove of the inner cylinder (3), three upper and lower equidistant worms (19) are arranged on the rotating shaft (18), and the three worms (19) are meshed with the three turbines (16) through tooth grooves respectively.

6. The production device for trichoderma fermentation according to claim 2, wherein the inner part of the kettle cover (10) is fixedly connected with a functional box (20), the inner part of the functional box (20) is provided with a liquid-separating temperature-regulating component (21), the liquid-separating temperature-regulating component (21) comprises a liquid-conveying pipe (2101) and a temperature-regulating sheet (2102), two ends of the liquid-conveying pipe (2101) are respectively provided with a liquid-sucking pipe (2103) and a liquid-conveying pipe (2104), the liquid-conveying pipe (2103) is movably connected with the liquid-returning pipe (701), the liquid-conveying pipe (2104) is movably connected with the liquid-conveying pipe (702), the liquid-conveying pipe (2101) is provided with a miniature liquid pump (2105), the miniature liquid pump (2105) is located between the liquid-sucking pipe (2103) and the liquid-conveying pipe (2104), the temperature-regulating sheet (2102) is located above the liquid-conveying pipe (2101), the temperature-regulating sheet (2102) is provided with a plurality of contact plates which are equidistant in circumference, and the contact plates are contacted with the liquid-conveying pipe (2101).

7. The production device for trichoderma fermentation according to claim 6, wherein the inner part of the inner cylinder (3) is provided with a spiral oxygen delivery pipe (22), the spiral oxygen delivery pipe (22) is positioned outside the three culture plates (8), the spiral oxygen delivery pipe (22) is fixedly connected with the inner wall of the inner cylinder (3), the side, close to the culture plates (8), of the spiral oxygen delivery pipe (22) is provided with an oxygen discharge groove (23), the kettle cover (10) is provided with a round hole, the inner part of the round hole is fixedly connected with an oxygen delivery pipe (24), the inner end of the oxygen delivery pipe (24) is movably connected with the spiral oxygen delivery pipe (22), and the outer port of the oxygen delivery pipe (24) is connected with an external oxygen delivery pipeline through a flange.

8. The production device for trichoderma fermentation according to claim 2, wherein the upper side of the kettle cover (10) is fixedly connected with a connecting seat (25), the connecting seat (25) is fixedly connected with a lifting frame (26), the lifting frame (26) is located on the outer side of the fermentation kettle body (1), one side, far away from the fermentation kettle body (1), of the lifting frame (26) is provided with a back toothed plate (27), one side of the fermentation kettle body (1) is provided with a portal frame (28), a chute is formed in the portal frame (28), the lifting frame (26) is movably connected with the portal frame (28), a motor support (29) is fixedly connected with a second reversing motor (30) on the portal frame (28), the output end of the second reversing motor (30) is connected with a short shaft through a coupling, the outer part of the short shaft is fixedly connected with a lifting gear (31), and the lifting gear (31) is meshed with the back toothed plate (27) through the toothed groove.

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