CN116159606A - Microfluidic detection system for refrigerator and refrigerator - Google Patents
Microfluidic detection system for refrigerator and refrigerator Download PDFInfo
- Publication number
- CN116159606A CN116159606A CN202111414254.4A CN202111414254A CN116159606A CN 116159606 A CN116159606 A CN 116159606A CN 202111414254 A CN202111414254 A CN 202111414254A CN 116159606 A CN116159606 A CN 116159606A
- Authority
- CN
- China
- Prior art keywords
- microfluidic
- detection
- main body
- air bag
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 133
- 238000000018 DNA microarray Methods 0.000 claims abstract description 74
- 239000007788 liquid Substances 0.000 claims abstract description 52
- 230000007246 mechanism Effects 0.000 claims abstract description 30
- 238000000034 method Methods 0.000 claims abstract description 15
- 230000008569 process Effects 0.000 claims abstract description 10
- 238000000071 blow moulding Methods 0.000 claims abstract description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims description 29
- 238000007789 sealing Methods 0.000 claims description 10
- 238000001125 extrusion Methods 0.000 claims description 4
- 238000002347 injection Methods 0.000 abstract description 7
- 239000007924 injection Substances 0.000 abstract description 7
- 238000004891 communication Methods 0.000 abstract description 6
- 238000005303 weighing Methods 0.000 description 23
- 239000000447 pesticide residue Substances 0.000 description 14
- 238000003860 storage Methods 0.000 description 8
- 238000003825 pressing Methods 0.000 description 7
- 238000013461 design Methods 0.000 description 6
- 230000033001 locomotion Effects 0.000 description 6
- 239000012530 fluid Substances 0.000 description 5
- 235000013305 food Nutrition 0.000 description 5
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 230000009471 action Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000002411 adverse Effects 0.000 description 3
- 230000001276 controlling effect Effects 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000009825 accumulation Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000005489 elastic deformation Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 235000020256 human milk Nutrition 0.000 description 1
- 210000004251 human milk Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 210000001503 joint Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000002572 peristaltic effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
-
- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F25—REFRIGERATION OR COOLING; COMBINED HEATING AND REFRIGERATION SYSTEMS; HEAT PUMP SYSTEMS; MANUFACTURE OR STORAGE OF ICE; LIQUEFACTION SOLIDIFICATION OF GASES
- F25D—REFRIGERATORS; COLD ROOMS; ICE-BOXES; COOLING OR FREEZING APPARATUS NOT OTHERWISE PROVIDED FOR
- F25D23/00—General constructional features
- F25D23/12—Arrangements of compartments additional to cooling compartments; Combinations of refrigerators with other equipment, e.g. stove
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/02—Adapting objects or devices to another
- B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
- B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
Abstract
The invention relates to a microfluidic detection system for a refrigerator and the refrigerator. The microfluidic detection system comprises: the microfluidic biochip is provided with a chip main body and an elastic air bag part which are integrally formed in a blow molding mode, wherein the chip main body is provided with a sample inlet, an air suction port and a detection pool, the sample inlet, the detection pool and the air suction port are sequentially communicated through a micro-channel, and the elastic air bag part is hermetically communicated with the air suction port; the sample liquid driving device is configured to controllably squeeze and release the elastic air bag part so as to promote the sample liquid contacted with the sample inlet to enter the micro-channel and flow to the detection pool through the micro-channel in the process of recovering deformation of the elastic air bag part; and the detection mechanism is used for detecting the detection pool so as to obtain preset detection parameters of the sample liquid. The sample liquid driving device and the microfluidic biochip do not need a communication pipeline, so that the problem of air tightness between the sample liquid driving device and the microfluidic biochip is solved, and the accuracy of sample injection control is maintained.
Description
Technical Field
The invention relates to a refrigeration technology, in particular to a microfluidic detection system for a refrigerator and the refrigerator.
Background
With the improvement of the living standard of people, pesticide residues, viruses, nutrient elements or other aspects of edible materials are generally required to be detected in daily life so as to qualitatively or quantitatively acquire the condition of the edible materials. For example, due to the problem of pesticide abuse, the daily purchased fruits and vegetables and agricultural and sideline products may have the problem of exceeding the pesticide residue content, and if the problem of exceeding the pesticide residue content of the foods cannot be timely found, the foods can cause great harm after being ingested by human bodies. For another example, breast feeding currently advocated is best feeding infants only if breast milk has normal nutritional value, however, the reduction of the content of nutrients in milk secreted by the infants and even the production of viruses may occur in the event of a sick, eaten, operated or otherwise by the lactating mother, thereby affecting the growth and health of the infants. The existing household appliances are single in function, and when pesticide residues, viruses, nutrient elements or other aspects of edible food materials are required to be detected, separate detection devices are required to be purchased, so that the quantity and the variety of the household appliances are various, the occupied space is large, and the intelligent household appliance does not accord with the development trend of intelligent families.
Among the detection methods, the method for detecting by using the microfluidic biochip is rapid, has small volume and is suitable for household use. In order to push the movement of the fluid in the chip, there are generally both pneumatic pushing and centrifugal pushing. Wherein centrifugal force pushing pushes liquid drops to flow by means of rotating centrifugal force, and unidirectional flow action can be regulated only by regulating the rotating speed. The air pressure pushing type utilizes positive and negative air pressure to bidirectionally push the fluid to move in the chip, and has high precision and strong controllability. However, when the air suction port of the chip is in butt joint with the air suction pipe of the pushing mechanism, the problems of unstable air tightness and unreliable caused by various reasons such as insufficient pressing area, uneven pressing surface, insufficient pressing force and insufficient precision of the piston of the injection pump can be avoided. To date, air tightness by air pressure pushing is still a technical problem which is not completely and effectively solved.
Disclosure of Invention
An object of the first aspect of the present invention is to overcome at least one of the drawbacks of the prior art, and to provide a microfluidic detection system with good sealing performance and accurate sample injection control, which is suitable for refrigerators.
A further object of the first aspect of the invention is to completely eliminate a series of adverse effects due to the problem of air tightness.
An object of a second aspect of the present invention is to provide a refrigerator having the microfluidic detection system described above.
According to a first aspect of the present invention there is provided a microfluidic detection system for a refrigerator comprising:
the microfluidic biochip comprises a chip main body and an elastic air bag part, wherein the chip main body is provided with a sample inlet, an air suction port and a detection pool formed in the chip main body, the sample inlet, the detection pool and the air suction port are sequentially communicated through a micro-channel, and the elastic air bag part is hermetically communicated with the air suction port;
a sample liquid driving device configured to controllably squeeze and release the elastic air bag portion to cause a sample liquid in contact with the sample inlet to enter the micro flow channel and flow to the detection cell through the micro flow channel in a process of restoring deformation of the elastic air bag portion; and
the detection mechanism is used for detecting the detection pool so as to obtain preset detection parameters of the sample liquid; wherein the method comprises the steps of
The chip main body and the elastic air bag part are integrally formed in a blow molding mode.
Optionally, a reagent adding hole communicated with the detection cell is formed on one side surface of the chip main body, so that a detection reagent is added into the detection cell through the reagent adding hole; and is also provided with
The microfluidic biochip further comprises a sealing patch sealingly attached to the one of the side surfaces of the chip body to close the reagent addition hole.
Alternatively, the elastic balloon portion has a screw shape or a corrugated shape extending in a length direction of the chip body; and is also provided with
The sample liquid driving device is configured to controllably apply an extrusion force parallel to an extending direction of the elastic balloon portion to cause the elastic balloon portion to elastically deform in the extending direction thereof.
Optionally, the sample inlet is located at the bottom of the chip main body, and the elastic air bag part is located at the top of the chip main body; and is also provided with
The sample liquid driving device is located above the microfluidic biochip and configured to controllably press the elastic balloon portion downward.
Optionally, the microfluidic detection system further comprises:
a chip mounting mechanism having a mounting groove for receiving the microfluidic biochip; and is also provided with
The microfluidic biochip is configured to be inserted into the mounting groove through a notch of the mounting groove, and a sample inlet of the chip main body is positioned outside the mounting groove.
Optionally, the sample inlet is located at the bottom of the chip main body, and the elastic air bag part is located at the top of the chip main body; and is also provided with
The mounting groove extends vertically, and the microfluidic biochip is configured to be inserted into the mounting groove in a direction parallel to a horizontal plane.
Optionally, the mounting groove includes a first groove section for accommodating the chip main body and a second groove section for accommodating the elastic air bag portion, the first groove section having a smaller size than the second groove section to form a step at a boundary of the first groove section and the second groove section; and is also provided with
The bottom of the elastic air bag part is abutted against the step part.
Optionally, the chip mounting mechanism further has at least one clamping member disposed within the mounting groove, the clamping member configured to clamp the chip body after the microfluidic biochip is inserted into the mounting groove.
Optionally, the clamping member comprises two symmetrical and spaced clamping jaws configured to apply opposing elastic forces to two opposing side surfaces of the chip body, respectively, after the microfluidic biochip is mounted to the mounting groove.
According to a second aspect of the present invention, the present invention further provides a refrigerator, which includes the microfluidic detection system according to any one of the above schemes.
The microfluidic detection system comprises a microfluidic biochip, wherein the microfluidic biochip is provided with a chip main body and an elastic air bag part, and the elastic air bag part is communicated with an air suction port of the chip main body in a sealing way, so that a closed space is formed inside the microfluidic biochip, and a through port for sample injection is reserved only at a sample injection port. The sample liquid driving device discharges air in the chip main body by extruding the elastic air bag part, and when the elastic air bag part is released by the sample liquid driving device, the elastic air bag part recovers deformation, so that the sample liquid contacted with the sample inlet is promoted to enter the detection pool in the chip main body. The microfluidic biochip is particularly designed with the elastic air bag part, a communication pipeline is not needed between the sample liquid driving device and the microfluidic biochip, only simple mechanical extrusion is performed, and the liquid suction and the air discharge are controlled by controlling the deformation of the elastic air bag part, so that the air tightness problem between the sample liquid driving device and the microfluidic biochip is eliminated, and the accuracy of sample injection control is maintained.
Further, the chip body and the elastic air bag part are integrally formed in a blow molding mode, that is, the microfluidic biochip is a component, the chip body and the elastic air bag part are only two different parts of the microfluidic biochip, and the chip body and the elastic air bag part do not need to be connected, so that the microfluidic biochip does not have any air tightness problem, and a series of adverse effects on the microfluidic detection system due to the air tightness problem are thoroughly eliminated.
According to the invention, the microfluidic detection system is integrated on the refrigerator, so that the storage function of the refrigerator is fully utilized, the detection process is more convenient, the microfluidic detection system and the refrigerator are convenient to carry out linkage control, the intelligent degree is higher, and the requirements of smart families are met.
The above, as well as additional objectives, advantages, and features of the present invention will become apparent to those skilled in the art from the following detailed description of a specific embodiment of the present invention when read in conjunction with the accompanying drawings.
Drawings
Some specific embodiments of the invention will be described in detail hereinafter by way of example and not by way of limitation with reference to the accompanying drawings. The same reference numbers will be used throughout the drawings to refer to the same or like parts or portions. It will be appreciated by those skilled in the art that the drawings are not necessarily drawn to scale. In the accompanying drawings:
fig. 1 is a schematic block diagram of a microfluidic detection system for a refrigerator according to one embodiment of the present invention;
fig. 2 is a schematic structural view of an internal structure of a microfluidic detection system according to one embodiment of the present invention;
fig. 3 is a partially schematic exploded view of a microfluidic detection system according to one embodiment of the present invention;
fig. 4 is a schematic cross-sectional view of a microfluidic biochip according to one embodiment of the invention;
fig. 5 is a schematic structural exploded view of a microfluidic biochip according to one embodiment of the invention;
fig. 6 is a schematic cross-sectional view of a microfluidic detection system according to one embodiment of the present invention in a partially structurally exploded state;
fig. 7 is a schematic structural view of a refrigerator according to an embodiment of the present invention.
Detailed Description
The invention firstly provides a micro-fluidic detection system for a refrigerator, which is used for qualitatively or quantitatively detecting preset detection parameters of a sample liquid, wherein the preset detection parameters can be pesticide residue parameters used for indicating whether the pesticide residue exceeds a standard and/or specific numerical values of the pesticide residue, nutrition parameters used for indicating whether the nutritional elements reach standards and/or specific contents of the nutritional elements, specific substance parameters used for indicating whether specific harmful substances (such as specific viruses) exceed the standard and/or specific contents, and the like.
Fig. 1 is a schematic structural view of a microfluidic detection system for a refrigerator according to one embodiment of the present invention, fig. 2 is a schematic structural view of an internal structure of the microfluidic detection system according to one embodiment of the present invention, fig. 3 is a partially schematic exploded view of a structure of the microfluidic detection system according to one embodiment of the present invention, and for convenience of understanding, a sample cup 2 is also shown in fig. 1 to 3.
Referring to fig. 1 to 3, the microfluidic detection system 1 of the present invention includes a microfluidic biochip 10, a sample liquid driving device 40, and a detection mechanism 20.
Fig. 4 is a schematic cross-sectional view of a microfluidic biochip according to one embodiment of the invention. The microfluidic biochip 10 has a chip main body 11 and an elastic airbag section 12, the chip main body 11 having a sample inlet 111, a gas inlet 112, and a detection cell 113 formed therein, the sample inlet 111, the detection cell 113, and the gas inlet 112 being sequentially communicated with each other through a microchannel 114, thereby forming a main channel. The elastic bag portion 12 is in sealed communication with the suction port 112. The sample liquid driving device 40 is configured to controllably squeeze and release the elastic balloon portion 12 to urge the sample liquid in contact with the sample inlet 111 into the micro flow channel 114 and flow to the detection cell 113 through the micro flow channel 114 in the process of restoring the deformation of the elastic balloon portion 12. The detection mechanism 20 is used for detecting the detection cell 113 to obtain preset detection parameters of the sample liquid. Specifically, a detection reagent may be provided in the detection cell 113 to detect the detection cell 113 by the detection mechanism 20 after the reaction of the sample liquid in the detection cell 113 and the detection reagent therein.
The microfluidic detection system 1 of the present invention includes a microfluidic biochip 10, the microfluidic biochip 10 having a chip body 11 and an elastic air bag portion 12, the elastic air bag portion 12 being in sealed communication with an air suction port 112 of the chip body 11, thereby forming a closed space inside the microfluidic biochip 10, and leaving a port for sample introduction only at a sample introduction port 111. The sample liquid driving device 40 discharges the air in the chip main body 11 by pressing the elastic air bag portion 12, and when the elastic air bag portion 12 is released by the sample liquid driving device 40, the elastic air bag portion 12 recovers its shape change, thereby causing the sample liquid in contact with the sample inlet 111 to enter the detection cell 113 in the chip main body 11 and react with the detection reagent in the detection cell 113. Further, the elastic air bag portion 12 can be repeatedly pressed and released by the sample liquid driving device 40 for a plurality of times, so that the sample liquid and the detection reagent are fully and uniformly mixed, and the accuracy of the detection result is improved.
The microfluidic biochip 10 of the present invention is specifically designed with the elastic air bag portion 12, no communication pipeline is needed between the sample liquid driving device 40 and the microfluidic biochip 10, only simple mechanical extrusion is performed, and the liquid suction and the gas discharge are controlled by controlling the deformation of the elastic air bag portion 12, so that the problem of air tightness between the sample liquid driving device 40 and the microfluidic biochip 10 is eliminated, and the accuracy of sample injection control is maintained.
It will be appreciated by those skilled in the art that the particular choice of microfluidic biochip 10 and detection mechanism 20 used may vary when the preset detection parameters for the microfluidic detection system are different. For example, when the microfluidic detection system is used for pesticide residue detection, the microfluidic biochip 10 may be a microfluidic pesticide residue detection chip capable of providing detection conditions for pesticide residue, and the detection mechanism 20 may be a pesticide residue detection mechanism capable of detecting pesticide residue parameters of pesticide residue.
In one particular embodiment, when the detection mechanism 20 is an agricultural residue detection mechanism for detecting agricultural residue parameters of agricultural residue, an enzyme inhibition method may be used to rapidly and qualitatively detect whether the agricultural residue of the sample liquid exceeds the standard. At this time, the chip body 11 further includes a reaction cell 115 formed therein, and the reaction cell 115 is located on a main channel formed by sequentially communicating the sample inlet 111, the detection cell 113, and the air inlet 112, and is communicated between the sample inlet 111 and the detection cell 113, so that the sample fluid reacts with the reactant in the reaction cell 115 and then flows into the detection cell 113. The reaction tank 115 and the sample inlet 111 and the reaction tank 115 and the detection tank 113 are communicated through the micro-channel 114. The reaction reagent and the detection reagent for pesticide residue detection can be respectively an enzyme reagent and a color reagent. The reaction cell 115 is used for reacting the sample liquid with the enzyme reagent therein, and the sample liquid after reacting with the enzyme reagent flows into the detection cell 113 to react with the color-developing agent in the detection cell 113. The detection mechanism 20 may alternatively be a photoelectric detection mechanism, which may include a light source, a photosensitive element, a heating plate, a temperature controller, and the like.
In some embodiments, the chip body 11 and the elastic balloon portion 12 are integrally molded by blow molding. That is, the microfluidic biochip 10 is a single component, the chip body 11 and the elastic balloon portion 12 of which are only two different portions of the microfluidic biochip 10, and the chip body 11 and the elastic balloon portion 12 do not need to be connected, and therefore, the microfluidic biochip 10 itself does not have any problem of air tightness, i.e., the addition of the elastic balloon portion 12 does not bring about the problem of air tightness to the microfluidic biochip 10 itself, thereby thoroughly eliminating a series of adverse effects to the microfluidic detection system 1 due to the problem of air tightness.
Fig. 5 is a schematic structural exploded view of a microfluidic biochip according to an embodiment of the invention. Since the chip body 11 and the elastic balloon portion 12 are integrally molded by blow molding, it is inconvenient to add a detection reagent to the detection cell 113 formed in the chip body 11 in advance. For this reason, in some embodiments, after the chip body 11 and the elastic balloon portion 12 are blow molded, a reagent addition hole 116 communicating with the detection cell 113 may be opened on one of the side surfaces 11a of the chip body 11 to add a detection reagent into the detection cell 113 through the reagent addition hole 116. When the reaction cell 115 is further formed in the chip body 11, a reaction reagent can be added to the reaction cell 115 in the same manner (i.e., by providing a reagent addition hole 117 communicating with the reaction cell 115 in the side surface of the chip body 11). Preferably, two reagent addition holes may be opened at the same side surface of the chip body 11 so as to seal the two reagent addition holes.
Further, the microfluidic biochip 10 further includes a sealing patch 13 sealingly attached to one of the side surfaces 11a of the chip body 11 (i.e., the side surface where the reagent addition hole is opened) to close the reagent addition hole 116 and the other reagent addition hole 117. Preferably, the above-described side surface 11a of the chip body 11 may be a side surface parallel to the width direction and the length direction thereof. This is because, since the surface area of the side surfaces parallel to the width direction and the length direction of the chip body 11 is large, it is convenient to form an adhesion surface with a large area between the chip body 11 and the seal patch 13, and the sealing effect between the two is improved. In addition, since the bonding between the chip body 11 and the sealing patch 13 is completed before the microfluidic biochip 10 is mounted, there is no limitation in the operation space and the sealing method, and therefore, an effective and good seal between the chip body 11 and the sealing patch 13 can be achieved.
It should be noted that the materials of the chip main body 11 and the elastic balloon portion 12 and the shape of the elastic balloon portion 12 are set so that the elastic balloon portion 12 can recover the deformation by its elastic deformation restoring force after the sample liquid driving device 40 releases the elastic balloon portion 12.
In some embodiments, the elastic balloon portion 12 has a screw shape or a corrugated shape extending along the length direction of the chip body 11, and the sample liquid driving device 40 is configured to controllably apply a pressing force parallel to the extending direction thereof to the elastic balloon portion 12 to cause the elastic balloon portion 12 to elastically deform along the extending direction thereof. That is, the direction of the squeezing force applied to the elastic bag portion 12 by the sample liquid driving device 40 coincides with the extending direction of the elastic bag portion 12. The elastic balloon portion 12 can be elastically contracted in the longitudinal direction of the chip body 11 by the pressing of the sample liquid driving device 40, and after the sample liquid driving device 40 releases the elastic balloon portion 12, the elastic balloon portion 12 can be reset by its own elasticity.
Specifically, the elastic balloon portion 12 may be a threaded tube or a bellows extending in the longitudinal direction of the chip body 11.
In some embodiments, the sample inlet 111 is located at the bottom of the chip body 11, and the elastic air bag portion 12 is located at the top of the chip body 11. The sample liquid driving device 40 is located above the microfluidic biochip 10 and configured to controllably press the elastic balloon portion 12 downward. That is, when the microfluidic biochip 10 is mounted, the length direction of the chip body 11 is a vertical direction, which facilitates both the contact of the sample inlet 111 with the sample liquid and the arrangement of the sample liquid driving device 40.
In some embodiments, the microfluidic detection system 1 further comprises a chip mounting mechanism 30. The applicant has realized that, since there is no air tightness problem between the microfluidic biochip 10 and the sample liquid driving device 40, that is, the sealing and abutting structure between the microfluidic biochip 10 and the sample liquid driving device 40 is not required to be considered when the microfluidic biochip 10 is mounted, it is only required to ensure that the microfluidic biochip 10 is stably and reliably mounted. Therefore, the chip mounting mechanism 30 of the present invention does not need to be designed into a very complex structure, and only needs to be able to hold the microfluidic biochip 10.
For this purpose, the chip mounting mechanism 30 of the present invention has a mounting groove 31 for receiving the microfluidic biochip 10. The microfluidic biochip 10 is configured to be inserted into the mounting groove 31 through the notch of the mounting groove 31, not only achieving efficient mounting of the microfluidic biochip 10, but also greatly simplifying the structure of the microfluidic detection system 1.
Further, the sample inlet 111 of the chip body 11 is located outside the mounting groove 31 to facilitate the sample inlet 111 to suck the sample liquid when the microfluidic biochip 10 is in a mounted state.
In some embodiments, the sample inlet 111 is located at the bottom of the chip body 11, and the elastic air bag portion 12 is located at the top of the chip body 11. Since the elastic balloon portion 12 is elastically contractible and deformable, the microfluidic biochip 10 is not easily installed from bottom to top.
For this purpose, the present invention further provides the mounting groove 31 to extend vertically, and the microfluidic biochip 10 is configured to be inserted into the mounting groove 31 in a direction parallel to the horizontal plane. That is, the elastic airbag portion 12 is installed in parallel with the chip body 11, the elastic airbag portion 12 does not cause any obstruction or influence on the assembly of the chip body 11, and at the same time, the chip body 11 is merely held stationary in the mounting groove 31 by the structural design of the mounting groove 31, allowing the elastic airbag portion 12 to be elastically deformed without obstruction in the mounting groove 31.
In some embodiments, the mounting groove 31 includes a first groove section 311 for receiving the chip body 11 and a second groove section 312 for receiving the elastic balloon portion 12, i.e., the first groove section 311 is located below the second groove section 312. The first groove section 311 has a smaller size than the second groove section 312 to form a step 32 at the boundary of the first groove section 311 and the second groove section 312. The bottom of the elastic air bag portion 12 abuts against the step portion 32 to prevent the microfluidic biochip 10 from falling down. Thereby, the entire microfluidic biochip 10 can be supported in the mounting groove 31, achieving positioning of the microfluidic biochip 10 in the vertical direction. The present invention positions the microfluidic biochip 10 in a vertical direction by a simple design of the structural dimensions of the mounting groove 31, and has a very simple structure.
Fig. 6 is a schematic cross-sectional view of a microfluidic detection system according to one embodiment of the present invention in a partially structurally exploded state. In some embodiments, the chip mounting mechanism 30 further has at least one clamping member 33 disposed in the mounting groove 31, and the clamping member 33 is configured to clamp the chip body 11 after the microfluidic biochip 10 is inserted into the mounting groove 31, so as to prevent the microfluidic biochip 10 from tilting, shaking or being detached from the mounting groove 31 during the process of pressing or releasing the elastic balloon portion 12 by the sample liquid driving device 40, thereby limiting the microfluidic biochip 10 in the horizontal direction.
Specifically, the mounting groove 31 may have a receiving space formed therein for receiving the clamping member 33, and the clamping member 33 is restricted in the receiving space and is elastically deformed within a certain range to maintain a preferable clamping force to the chip body 11.
Further, the clamping member 33 may include two symmetrical and spaced-apart clamping jaws 331, and the two clamping jaws 331 are configured to apply elastic forces in opposite directions to two opposite side surfaces of the chip body 11, respectively, after the microfluidic biochip 10 is mounted to the mounting groove 31, thereby more smoothly holding the microfluidic biochip 10.
In some embodiments, the sample fluid drive device 40 includes a drive motor 41 and a push rod 42. The push rod 42 is connected to the drive motor 41 and is configured to translate along an output shaft of the drive motor 41 when the drive motor 41 rotates. Specifically, the output shaft of the driving motor 41 may be parallel to the extending direction of the elastic balloon portion 12 to press the elastic balloon portion 12 or release the elastic balloon portion 12 with the push rod 42 when the driving motor 41 rotates.
In some embodiments, the microfluidic detection system 1 further comprises a weighing station 81 and a carriage 82. The weighing stage 81 is fixedly arranged on a support 83 and serves to measure the weight of a sample contained in the sample cup 2 placed thereon. It will be appreciated that the weighing station 81 may measure the sum of the weights of the sample cup 2 and the sample contained therein, and subtract the weight of the sample cup 2 itself to obtain the weight of the sample. The weighing station 81 may also be arranged to directly detect the weight of the sample contained in the sample cup 2, for example a peeling measurement. The carriage 82 is configured to controllably or operatively move to bring the sample cup 2 into a highest position allowing sample fluid in the sample cup 2 to contact the sample inlet 111 of the microfluidic biochip 10.
In some embodiments, the microfluidic detection system 1 further comprises a buffer bottle 51 and a buffer drive 52. The buffer bottle 51 is for containing a buffer. The buffer driving device 52 is in communication with the buffer bottle 51 to controllably drive the buffer in the buffer bottle 51 into the sample cup 2 placed on the weighing table 81, so that the buffer is mixed with the sample in the sample cup 2 to generate the sample liquid. In particular, buffer drive 52 may be a peristaltic pump, a diaphragm pump, or other suitable type of drive.
In some embodiments, the microfluidic detection system 1 further comprises a housing 90. The housing 90 has formed thereon an operation table open toward a front side thereof, and the weighing table 81 is at least partially located in the operation table, thereby facilitating the user's operations of placing the sample cup 2, taking out the sample cup 2, and the like in the operation table.
The microfluidic detection system 1 of the present invention is in particular provided with a weighing station 81 fixed on a support 83 and a carriage 82 capable of moving the sample cup 2. When detecting, the user only needs to place sample cup 2 on weighing platform 81, and weighing platform 81 measures the weight of sample, and buffer drive arrangement 52 adds appropriate amount of buffer in to sample cup 2, and bracket 82 can drive sample cup 2 automatically and remove in order to apply a sample to microfluidic biochip 10, and the application of sample operation is very convenient, labour saving and time saving, and user's use experience is better. More importantly, the weighing platform 81 is fixed and does not move along with the movement of the bracket 82, so that the movement of the bracket 82 does not have any influence on the weighing precision of the weighing platform 81, high-precision measurement of the weight of a sample is ensured, and the accuracy of the detection result of the microfluidic biochip 10 is further improved.
The inventors have realized that when the sample cup 2 is placed on the weighing station 81 for weighing, the carrier 82 should be completely disengaged from, and out of contact with, the sample cup 2 to avoid having an impact on the weighing of the sample. After the weight of the sample has been measured, the carrier 82 needs to have a holding action on the sample cup 2 to bring it together. That is, the holder 82 needs to have two states of releasing the sample cup and holding the sample cup, and can be automatically switched between these two states according to the detection progress. To achieve this, prior to the present application, the design concept commonly adopted by those skilled in the art was to provide a clamping mechanism for the carrier, and to automatically switch the carrier between two states of releasing the sample cup and holding the sample cup by controlling the action of the clamping mechanism. However, the applicant has appreciated that such conventional design concepts are relatively late and suffer from a number of drawbacks. For example, the clamping mechanism increases the structural complexity of the bracket and requires space for switching the motion of the clamping mechanism to avoid interference or collision with other structures, which all results in an increased volume of the microfluidic detection system, which is not suitable for refrigerators with limited space. For another example, the holding of the sample cup, in particular the release of the sample cup, needs to be kept highly consistent with the detection process, i.e. when the weighing station needs to measure the weight of the sample, it must be ensured that the clamping mechanism is in a state of releasing the sample cup; the clamping mechanism can clamp the sample cup only after the weighing platform finishes measuring the weight of the sample, the time control precision requirement for the state switching of the clamping mechanism is very high, and if the state switching of the clamping mechanism is slightly deviated or error accumulation occurs, the whole detection flow is easily disturbed, so that a correct detection result is not obtained.
Therefore, the inventor tries to break through the traditional design thought and designs a brand new bracket structure. In some embodiments, the bracket 82 is disposed above the weigh table 81 and includes an annular frame 821 that fits over the exterior of the sample cup 2. The carriage 82 is configured to controllably or operatively move in an up-and-down direction and, upon upward movement, to hold the sample cup 2 up with the annular frame 821 such that the sample cup 2 leaves the weighing station 81, supports the sample cup 2 on the weighing station 81 during downward movement to a lowermost position, and urges the sample cup 2 out of engagement with the annular frame 821 with the abutment between the sample cup 2 and the weighing station 81.
That is, as the carriage 82 moves upward, the annular frame 821 can naturally hold the sample cup 2 off the weighing table 81; when the bracket 82 moves downward to a certain position, the sample cup 2 is supported on the weighing platform 81, and the abutment action between the sample cup 2 and the weighing platform 81 is utilized to promote the sample cup 2 to be separated from the annular frame 821 during the process that the bracket 82 continues to move downward to the lowest position, so that the bracket 82 does not have any influence on the weight detection of the sample. It can be seen that the bracket 82 of the present invention completes the natural switching between the lifting and releasing of the sample cup 2 during the lifting process, and no control program for lifting or releasing is required to be designed, so that the structure of the bracket 82 is very simple, and the control logic of the bracket 82 is also very simple.
The present invention also provides a refrigerator, and fig. 7 is a schematic structural view of a refrigerator according to an embodiment of the present invention. The refrigerator 100 of the present invention includes the microfluidic detection system 1 according to any of the above embodiments, so as to integrate the microfluidic detection system 1 on the refrigerator 100. The refrigerator 100 has a high frequency of use in daily life, and the refrigerator 100 is mainly used for storing food, and when the microfluidic detection system 1 is integrated on the refrigerator 100, a user can conveniently perform a detection operation of a food sample using the microfluidic detection system 1.
According to the invention, the microfluidic detection system 1 is integrated on the refrigerator 100, so that the storage function of the refrigerator 100 is fully utilized, the detection process is more convenient, the microfluidic detection system 1 and the refrigerator 100 are convenient to carry out linkage control, the intelligent degree is higher, and the requirements of smart families are met.
Further, the refrigerator 100 further includes a case 200 and a door 300, wherein a storage space is defined in the case 200, and the door 300 is connected to the case 200 and is used to open and/or close the storage space. The microfluidic detection system 1 is preferably arranged on the door 300, so that the operation is convenient, the original storage space in the refrigerator 200 is not occupied, and the storage capacity of the refrigerator 100 is not affected.
The refrigerator 100 of the present application is a refrigerator in a broad sense, which includes not only a refrigerator in a so-called narrow sense, but also a storage device having a refrigerating, freezing or other storage function, for example, a refrigerator, a freezer, etc.
It should be further understood by those skilled in the art that terms such as "upper", "lower", "front", "rear", "top", "bottom", etc. used to indicate the orientation or positional relationship in the embodiments of the present invention are based on the actual use states of the microfluidic detection system 1 and the refrigerator 100, and these terms are merely for convenience of description and understanding of the technical solutions of the present invention, and are not meant to indicate or imply that the device referred to or not necessarily have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
By now it should be appreciated by those skilled in the art that while a number of exemplary embodiments of the invention have been shown and described herein in detail, many other variations or modifications of the invention consistent with the principles of the invention may be directly ascertained or inferred from the present disclosure without departing from the spirit and scope of the invention. Accordingly, the scope of the present invention should be understood and deemed to cover all such other variations or modifications.
Claims (10)
1. A microfluidic detection system for a refrigerator, comprising:
the microfluidic biochip comprises a chip main body and an elastic air bag part, wherein the chip main body is provided with a sample inlet, an air suction port and a detection pool formed in the chip main body, the sample inlet, the detection pool and the air suction port are sequentially communicated through a micro-channel, and the elastic air bag part is hermetically communicated with the air suction port;
a sample liquid driving device configured to controllably squeeze and release the elastic air bag portion to cause a sample liquid in contact with the sample inlet to enter the micro flow channel and flow to the detection cell through the micro flow channel in a process of restoring deformation of the elastic air bag portion; and
the detection mechanism is used for detecting the detection pool so as to obtain preset detection parameters of the sample liquid; wherein the method comprises the steps of
The chip main body and the elastic air bag part are integrally formed in a blow molding mode.
2. The microfluidic detection system according to claim 1, wherein,
a reagent adding hole communicated with the detection pool is formed in one side surface of the chip main body, so that detection reagent is added into the detection pool through the reagent adding hole; and is also provided with
The microfluidic biochip further comprises a sealing patch sealingly attached to the one of the side surfaces of the chip body to close the reagent addition hole.
3. The microfluidic detection system according to claim 1, wherein,
the elastic air bag part has a thread shape or a corrugated shape extending in a length direction of the chip main body; and is also provided with
The sample liquid driving device is configured to controllably apply an extrusion force parallel to an extending direction of the elastic balloon portion to cause the elastic balloon portion to elastically deform in the extending direction thereof.
4. The microfluidic detection system according to claim 3, wherein,
the sample inlet is positioned at the bottom of the chip main body, and the elastic air bag part is positioned at the top of the chip main body; and is also provided with
The sample liquid driving device is located above the microfluidic biochip and configured to controllably press the elastic balloon portion downward.
5. The microfluidic detection system of claim 1, further comprising:
a chip mounting mechanism having a mounting groove for receiving the microfluidic biochip; and is also provided with
The microfluidic biochip is configured to be inserted into the mounting groove through a notch of the mounting groove, and a sample inlet of the chip main body is positioned outside the mounting groove.
6. The microfluidic detection system according to claim 5, wherein,
the sample inlet is positioned at the bottom of the chip main body, and the elastic air bag part is positioned at the top of the chip main body; and is also provided with
The mounting groove extends vertically, and the microfluidic biochip is configured to be inserted into the mounting groove in a direction parallel to a horizontal plane.
7. The microfluidic detection system according to claim 6, wherein,
the mounting groove comprises a first groove section for accommodating the chip main body and a second groove section for accommodating the elastic air bag part, and the size of the first groove section is smaller than that of the second groove section so as to form a step part at the boundary of the first groove section and the second groove section; and is also provided with
The bottom of the elastic air bag part is abutted against the step part.
8. The microfluidic detection system according to claim 5, wherein,
the chip mounting mechanism further has at least one clamping member disposed within the mounting slot, the clamping member configured to clamp the chip body after the microfluidic biochip is inserted into the mounting slot.
9. The microfluidic detection system according to claim 8, wherein,
the clamping piece comprises two clamping jaws which are symmetrical and are arranged at intervals, and the two clamping jaws are configured to respectively apply opposite elastic acting forces to two opposite side surfaces of the chip main body after the microfluidic biochip is mounted to the mounting groove.
10. A refrigerator comprising a microfluidic detection system according to any one of claims 1 to 9.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111414254.4A CN116159606A (en) | 2021-11-25 | 2021-11-25 | Microfluidic detection system for refrigerator and refrigerator |
| PCT/CN2022/126154 WO2023093383A1 (en) | 2021-11-25 | 2022-10-19 | Microfluidic detection system for refrigerator, and refrigerator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111414254.4A CN116159606A (en) | 2021-11-25 | 2021-11-25 | Microfluidic detection system for refrigerator and refrigerator |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116159606A true CN116159606A (en) | 2023-05-26 |
Family
ID=86415071
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111414254.4A Pending CN116159606A (en) | 2021-11-25 | 2021-11-25 | Microfluidic detection system for refrigerator and refrigerator |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN116159606A (en) |
| WO (1) | WO2023093383A1 (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117214453B (en) * | 2023-11-07 | 2024-04-05 | 长春迈克赛德医疗科技有限公司 | Sample suction needle system and sample suction method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205731290U (en) * | 2015-12-23 | 2016-11-30 | 杭州霆科生物科技有限公司 | A kind of food safety detection micro-fluidic chip of pre-stored reaction reagent |
| CN207393445U (en) * | 2017-11-10 | 2018-05-22 | 威海波斯顿游艇股份有限公司 | A kind of manual-automatic integrated air charging system based on motorboat |
| CN208031626U (en) * | 2017-12-07 | 2018-11-02 | 李刚 | A kind of portable simple respirator |
| CN212674733U (en) * | 2020-07-01 | 2021-03-09 | 江西远东生物科技有限公司 | Detection equipment for producing medical disposable mask |
| CN214041434U (en) * | 2020-09-27 | 2021-08-24 | 青岛海尔电冰箱有限公司 | Micro-fluidic detection system for refrigerator and refrigerator |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102671729B (en) * | 2012-05-07 | 2014-04-16 | 博奥生物有限公司 | Micro-fluidic chip for multi-index biochemical detection |
| CN204514801U (en) * | 2015-04-01 | 2015-07-29 | 杭州霆科生物科技有限公司 | A kind of throwing type micro-fluidic chip for residues of pesticides Site Detection |
| US20160310948A1 (en) * | 2015-04-24 | 2016-10-27 | Mesa Biotech, Inc. | Fluidic Test Cassette |
| CN214039171U (en) * | 2020-09-27 | 2021-08-24 | 青岛海尔电冰箱有限公司 | Micro-fluidic detection system for refrigerator and refrigerator |
-
2021
- 2021-11-25 CN CN202111414254.4A patent/CN116159606A/en active Pending
-
2022
- 2022-10-19 WO PCT/CN2022/126154 patent/WO2023093383A1/en unknown
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN205731290U (en) * | 2015-12-23 | 2016-11-30 | 杭州霆科生物科技有限公司 | A kind of food safety detection micro-fluidic chip of pre-stored reaction reagent |
| CN207393445U (en) * | 2017-11-10 | 2018-05-22 | 威海波斯顿游艇股份有限公司 | A kind of manual-automatic integrated air charging system based on motorboat |
| CN208031626U (en) * | 2017-12-07 | 2018-11-02 | 李刚 | A kind of portable simple respirator |
| CN212674733U (en) * | 2020-07-01 | 2021-03-09 | 江西远东生物科技有限公司 | Detection equipment for producing medical disposable mask |
| CN214041434U (en) * | 2020-09-27 | 2021-08-24 | 青岛海尔电冰箱有限公司 | Micro-fluidic detection system for refrigerator and refrigerator |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2023093383A1 (en) | 2023-06-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN214039111U (en) | Refrigerator with a door | |
| EP2925448B1 (en) | Device and method for carrying out an assay to detect a nucleic acid in a sample | |
| AU2004312764B2 (en) | Apparatus for modifying pressure within a fluid dispenser | |
| CN214039171U (en) | Micro-fluidic detection system for refrigerator and refrigerator | |
| WO2022062909A1 (en) | Refrigerator | |
| CN214041434U (en) | Micro-fluidic detection system for refrigerator and refrigerator | |
| EP1333287B1 (en) | Method and apparatus for modifying pressure within a fluid dispenser | |
| CN116159606A (en) | Microfluidic detection system for refrigerator and refrigerator | |
| CN214039173U (en) | Micro-fluidic detection system for refrigerator and refrigerator | |
| WO2022062994A1 (en) | Microfluidic detection system, control method therefor, and refrigerator | |
| US20230366906A1 (en) | Microfluidic detection system for refrigerator and refrigerator | |
| CN114324904A (en) | Micro-fluidic detection system for refrigerator and refrigerator | |
| CA2676751A1 (en) | Method and dosing device for dosing a fluid into a receiving channel of a test element for analyzing body fluids | |
| CN205361370U (en) | Micro -fluidic chip | |
| CN116159607A (en) | Microfluidic detection system for refrigerator and refrigerator | |
| CN105214745A (en) | A kind of micro-fluidic chip | |
| CN114324909A (en) | Micro-fluidic detection system for refrigerator and refrigerator | |
| CN114324913A (en) | Micro-fluidic detection system for refrigerator and refrigerator | |
| CN115876753A (en) | Micro-fluidic detection system for refrigerator and refrigerator | |
| CN115901734A (en) | Micro-fluidic detection system for refrigerator and refrigerator | |
| CN114279136A (en) | Refrigerator with a door | |
| CN211996634U (en) | Quantitative liquid taking vacuum biological reagent storage bottle | |
| US20230366903A1 (en) | Microfluidic testing system and control method therefor, and refrigerator | |
| CN114324903A (en) | Micro-fluidic detection system for refrigerator, control method of micro-fluidic detection system and refrigerator | |
| US20110171725A1 (en) | Mechanism and method of preventing suction air from leaking during filtration of capturing carrier solution |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |