CN116115683B - Honeysuckle antibacterial liquid and preparation method thereof - Google Patents
Honeysuckle antibacterial liquid and preparation method thereof Download PDFInfo
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- CN116115683B CN116115683B CN202310044135.7A CN202310044135A CN116115683B CN 116115683 B CN116115683 B CN 116115683B CN 202310044135 A CN202310044135 A CN 202310044135A CN 116115683 B CN116115683 B CN 116115683B
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- parts
- honeysuckle
- fatty alcohol
- filtrate
- antibacterial
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- A61K2236/30—Extraction of the material
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Abstract
The application relates to the field of biological medicine, and in particular discloses a honeysuckle antibacterial liquid and a preparation method thereof. The honeysuckle antibacterial liquid comprises the following raw materials in parts by weight: 20-50 parts of honeysuckle, 20-40 parts of fructus cnidii, 15-35 parts of radix sophorae flavescentis, 12-18 parts of pepper, 140-160 parts of boric acid, 20-30 parts of borax and 20-30 parts of chlorhexidine acetate; the preparation method comprises the steps of water extraction, vacuum concentration, alcohol precipitation concentration, filtration, addition of other auxiliary agents and uniform stirring. The honeysuckle antibacterial liquid can be used for inhibiting growth of ear germs and treating ear inflammation, and has the advantages of high antibacterial rate, high temperature and no irritation.
Description
Technical Field
The application relates to the field of biological medicine, in particular to a honeysuckle antibacterial liquid and a preparation method thereof.
Background
The ear is an important auditory organ of humans, including the outer, middle and inner ear, where auditory and bit receptors are located. The external ear comprises auricle and external auditory canal, and the skin of the external auditory canal is provided with auricle and glands, and secretion of glands and auricle have certain blocking effect on foreign matters such as external dust. It is also because the cavity structure of the ear is easy to cause the inside of the ear to be a sanitary dead angle, and accumulated dust, gland secretion and the like are easy to cause infection and inflammation in the ear.
In modern medicine, for various infections and inflammations, mainly sterilization and drug treatment are performed, for example, antibiotics are used, and although the effects of temporary pain relieving and inflammation diminishing can be achieved, the large amount of antibiotics are certainly 'exercise' for the drug resistance of pathogenic bacteria, and meanwhile most common bacteria are killed, pathogenic bacteria with drug resistance which are not dominant originally remain and are multiplied in a large amount. And because the medicine is stimulated for a long time, part of pathogenic bacteria are mutated and become drug-resistant strains, and repeated infection is caused. The effect of the chemical antibiotic ear drops is not obvious, some aminoglycoside antibiotics have direct toxic effect on the inner ear, damage the cochlea and influence the hearing, and cause the patient to have drug-induced deafness, while the traditional Chinese medicine external medicine is mainly powder, ointment and oil preparations, and the ear wax is easy to generate and difficult to eliminate after the patient uses the medicine, thus influencing the hearing, and further limiting the clinical medication.
In view of the above-mentioned problems, the inventors consider that the drugs for ear administration at present have disadvantages of inconvenient use and susceptibility to drug resistance.
Disclosure of Invention
In order to obtain a bacteriostatic liquid capable of effectively inhibiting pathogenic bacteria in ears, the application provides a honeysuckle bacteriostatic liquid and a preparation method thereof.
In a first aspect, the present application provides a honeysuckle antibacterial solution and a preparation method thereof, and adopts the following technical scheme: the honeysuckle antibacterial liquid comprises the following raw materials in parts by weight: 20-50 parts of honeysuckle, 20-40 parts of fructus cnidii, 15-35 parts of radix sophorae flavescentis, 12-18 parts of pepper, 140-160 parts of boric acid, 20-30 parts of borax and 20-30 parts of chlorhexidine acetate.
Honeysuckle flower has cold property, and the main components of the honeysuckle flower comprise flavonoid substances, terpenoid compounds, triterpenoid soap substances, organic acid substances and the like, so that the honeysuckle flower has the inhibition effect on bacteria and fungi, particularly chlorogenic acid with the highest content in organic acid has very strong bactericidal effect, can play an obvious inhibition effect on bacteria in vivo and in vitro, and has broad-spectrum antibacterial property.
The fructus cnidii has the effects of eliminating dampness, dispelling wind, killing parasites, relieving itching, and has strong antiallergic capability, and relieving itching symptoms, and the fructus cnidii contains osthole, isopimpingetin, bergapten and the like, and can inhibit the generation, germination, adhesion and invasion of pathogenic bacteria spores by inhibiting chitin deposition on fungal cell walls.
The kuh-seng has cold property, and alkaloid, flavonoid and phenol compounds contained in the kuh-seng can kill and inhibit various fungi, bacteria and viruses, and the bactericidal active ingredients of the kuh-seng can inhibit the biosynthesis of thalli and influence the biological oxidation process of mycelium, so that the fungi are killed, the growth of the fungi is inhibited, and the bactericidal composition has broad-spectrum bactericidal property. However, the biological activity of any individual component is very low, so the compound preparation is used with honeysuckle and kuh-seng which can increase the permeability of cell membranes, and the good antibacterial and bactericidal effects of the compound preparation are effectively improved.
The pepper is warm and contains laurocapram, dimethyl sulfoxide, limonene and the like, and the components can inhibit fungi, enter fungi cells and combine with substances participating in bacterial reproduction to accelerate the death of the fungi.
Boric acid and borax have bactericidal capability, and boric acid-borax buffer solution formed by compounding the boric acid and borax can balance the antibacterial solution to be consistent with the environmental conditions such as pH value, osmotic pressure and the like in the cell growth environment, so that the antibacterial solution is milder, does not generate irritation and achieves good antibacterial effect.
Chlorhexidine acetate is adsorbed on the permeable barrier of bacterial plasma membrane to leak cell content, inhibit and kill pathogenic bacteria, and has the advantages of broad antibacterial spectrum and strong antibacterial effect.
By adopting the technical scheme, boric acid and borax solution are used as buffer solution to reduce the irritation of the antibacterial solution to skin while inhibiting bacteria, and chlorhexidine acetate cooperates with kuh-seng and honeysuckle to change the permeability of germ cell membranes, so that the active ingredients in the antibacterial solution can play a role more easily, thereby achieving good sterilization and bacteriostasis effects.
Optionally, the raw materials further comprise 8-12 parts by weight of citric acid higher fatty alcohol ester.
Optionally, the citric acid higher fatty alcohol ester is fatty alcohol polyethylene citrate.
By adopting the technical scheme, the fatty alcohol polyethylene citrate added in the application is nonionic in surface activity and is matched with chlorhexidine acetate with cationic surface activity to generate a synergistic effect. Meanwhile, the fatty alcohol polyethylene citrate has good adaptability to skin, improves the comfort level of using antibacterial liquid, and can repair damaged skin caused by infection and inflammation.
Optionally, the fatty alcohol polyethylene citrate in the raw materials is prepared by the following steps:
(1) Citric acid and fatty alcohol polyoxyethylene ether in a proportion of 1: (1.5-2.0), adding a catalyst, controlling the reaction temperature within 140-160 ℃ and fully reacting;
(2) And after the reaction is finished, performing reduced pressure rotary evaporation, and fully removing water to obtain the fatty alcohol polyethylene citrate.
By adopting the technical scheme, the proportion of the citric acid to the fatty alcohol-polyoxyethylene ether and the reaction temperature are controlled, so that the main product generated by the reaction is citric acid monoester, and the citric acid monoester and chlorhexidine acetate are compounded to prepare the mild and non-irritating antibacterial liquid.
Optionally, the raw materials further comprise 10-15 parts by weight of phenyllactic acid.
By adopting the technical scheme, the phenyllactic acid is a natural bacteriostatic agent, has no toxicity and broad bacteriostasis spectrum, and can effectively improve the bacteriostatic activity by being combined with chlorhexidine acetate, so that a synergistic bacteriostatic effect is generated, and the fungal cells enter the inside of the cells to be combined with DNA to lose the reproductive growth activity of the fungi, so that the effect of killing and inhibiting is achieved.
Optionally, the honeysuckle antibacterial liquid comprises the following raw materials in parts by weight: 25-35 parts of honeysuckle, 25-35 parts of fructus cnidii, 25-30 parts of radix sophorae flavescentis, 13-16 parts of pepper, 145-155 parts of boric acid, 22-26 parts of chlorhexidine acetate, 12-14 parts of phenyllactic acid and 9-11 parts of fatty alcohol polyethylene citrate.
In a second aspect, the present application provides a preparation method of a honeysuckle antibacterial solution, which adopts the following technical scheme:
a preparation method of the honeysuckle antibacterial liquid comprises the following operation steps:
(1) Weighing flos Lonicerae, radix Sophorae Flavescentis and fructus Zanthoxyli according to weight, mixing, wrapping fructus Cnidii with cotton cloth, extracting at one time, adding 7-8 times of water relative to the traditional Chinese medicinal materials, heating to keep slight boiling, extracting and refluxing for 0.8-1.5 hr, and filtering with screen; adding water into the filter residue, repeating the steps, and combining the filtrates of the two extractions to obtain filtrate A;
(2) Concentrating the filtrate A in vacuum, and standing at normal temperature to obtain concentrated extract;
(3) Slowly adding ethanol into the concentrated extract, stirring while controlling the temperature to be less than or equal to 10 ℃, standing for 11-12h, filtering the supernatant, collecting filtrate, and discarding precipitate to obtain filtrate B;
(4) Weighing corresponding amounts of boric acid, borax and chlorhexidine acetate, respectively adding 2-3 times of purified water, stirring to dissolve completely, mixing together, and filtering to obtain mixed solution;
(5) Mixing the filtrate B with the mixed solution, adding purified water to 19-21 times of the raw materials and auxiliary materials, stirring uniformly, regulating the pH value to 5-7 with hydrochloric acid, and stirring uniformly to obtain the honeysuckle antibacterial solution.
By adopting the technical scheme, the beneficial components in the traditional Chinese medicine raw materials are fully extracted by using the methods of water extraction, vacuum concentration under reduced pressure and alcohol precipitation concentration, then are uniformly mixed with the fully dissolved and filtered boric acid, borax and chlorhexidine acetate components, and the pH of the antibacterial solution is diluted and regulated to be slightly acidic so as to change the alkaline environment of bacterial growth and accelerate the death of the bacteria.
Preferably, in the step (3), 95% ethanol is added, and the addition is stopped when the ethanol concentration of the mixed solution is 75% while stirring.
In summary, the present application has the following beneficial effects:
1. because the application adopts the honeysuckle, the fructus cnidii, the radix sophorae flavescentis and the pepper, the good sterilization characteristics of the traditional Chinese medicines are fully utilized, and the antibacterial capability of borax, boric acid and chlorhexidine acetate is cooperated, the bacterial structure is destroyed by increasing the permeability of bacterial and fungal cell membranes, and the functional disorder such as electron transfer, nutrition absorption, nucleotide synthesis and ATP activity of the membranes is caused by the release of intracellular components of microbial cells, so that the effects of killing bacteria and inhibiting growth are achieved. Meanwhile, through a specific formula and a specific proportion, the antibacterial liquid has the effects of warming the middle-jiao and dispelling cold, the fructus cnidii and the Chinese prickly ash are warm in nature, the honeysuckle and the kuh-seng are cold in nature, and the antibacterial liquid is matched with each other to neutralize the drug property of the antibacterial liquid without affecting the sterilization and bacteriostasis effects of the antibacterial liquid, so that the condition of causing discomfort of organisms is avoided. The prepared antibacterial agent with antibacterial broad spectrum and no drug resistance not only can kill pathogenic microorganisms, but also can mobilize the stress capability of organisms, improve the immune function and disease resistance defense function of the organisms, and the organisms are not easy to generate drug resistance.
2. In the application, citric acid higher fatty alcohol ester and phenyllactic acid are preferably added, the citric acid higher fatty alcohol ester and phenyllactic acid can be used for generating synergistic improvement of sterilization and bacteriostasis effects with chlorhexidine acetate, and meanwhile, the damaged skin can be protected and irritation can be avoided due to the addition of the citric acid higher fatty alcohol ester.
3. According to the method, the beneficial components in the traditional Chinese medicinal materials are fully extracted by controlling the processes in the water extraction and alcohol precipitation processes, the utilization rate of the raw medicinal materials is improved, the effective components are more completely leached, and the antibacterial liquid with the concentration suitable for ear bacteriostasis is obtained after dilution and pH adjustment.
Detailed Description
The present application is described in further detail below with reference to examples.
Fatty alcohol polyoxyethylene ether, available from ataxia blueprint chemical company.
Preparation of fatty alcohol polyethylene citrate:
preparation example 1
(1) Mixing 2kg of citric acid and 3kg of fatty alcohol polyoxyethylene ether uniformly, adding aluminum oxide as a catalyst, controlling the reaction temperature to 160 ℃, and heating until no water is generated to finish the reaction;
(2) And after the reaction is finished, adding acetone, decompressing, rotating and evaporating, and fully removing water to obtain the fatty alcohol polyethylene citrate.
Preparation example 2
(1) 2kg of citric acid and 4kg of fatty alcohol polyoxyethylene ether in a proportion of 1: mixing uniformly in the proportion of (1.5-2.0), adding a catalyst, controlling the reaction temperature at 180 ℃, and stopping heating to finish the reaction when no water is generated;
(2) And after the reaction is finished, adding acetone, decompressing, rotating and evaporating, and fully removing water to obtain the fatty alcohol polyethylene citrate.
Examples
Example 1
The honeysuckle antibacterial liquid is prepared from the following raw materials in parts by weight as shown in table 1:
(1) Weighing 20g of honeysuckle, 40g of fructus cnidii, 15g of radix sophorae flavescentis and 18g of pepper according to the weight, wrapping the fructus cnidii with cotton cloth, mixing the wrapped fructus cnidii with other medicinal materials, extracting and adding 651g of water for one time, heating and keeping micro-boiling, extracting and refluxing for 0.8h, and filtering the liquid medicine through a 200-mesh screen; adding 744g of water into filter residues in the secondary extraction, repeating the primary extraction step, and combining the filtrates of the two extractions to obtain filtrate A;
(2) Vacuum concentrating the filtrate A, controlling vacuum concentration vacuum degree to-0.07 MPa, concentrating at 75deg.C until the relative density is 1.20, and standing at normal temperature to obtain concentrated extract;
(3) Slowly adding 95% ethanol into the concentrated extract, stirring until the ethanol concentration of the mixed solution is 75%, standing at a temperature lower than 10deg.C for 11 hr, filtering the supernatant, collecting filtrate, and discarding the precipitate to obtain filtrate B;
(4) Weighing 140g of boric acid, 20g of borax and 20g of chlorhexidine acetate, respectively adding 280g of purified water, 40g of purified water and 40g of purified water, stirring until the purified water is fully dissolved, mixing the two components together, and filtering to obtain a mixed solution;
(5) Mixing the filtrate B with the mixed solution, adding purified water until the total amount reaches 5.2kg, stirring uniformly, adjusting the pH value to 5 with hydrochloric acid, and stirring for 30min to obtain the honeysuckle antibacterial solution.
Example 2
The honeysuckle antibacterial liquid is prepared from the following raw materials in parts by weight as shown in table 1:
(1) Weighing 30g of honeysuckle, 30g of fructus cnidii, 30g of radix sophorae flavescentis and 15g of pepper according to the weight, wrapping the fructus cnidii with cotton cloth, mixing the wrapped fructus cnidii with other medicinal materials, extracting and adding 840g of water for one time, heating and keeping micro-boiling, extracting and refluxing for 1 hour, and filtering the liquid medicine through a 200-mesh screen; adding 840g of water into filter residues in the secondary extraction, repeating the primary extraction step, and combining the filtrates of the two extractions to obtain filtrate A;
(2) Vacuum concentrating the filtrate A, controlling vacuum concentration degree to-0.06 MPa, concentrating at 70deg.C until the relative density is 1.15, and standing at normal temperature to obtain concentrated extract;
(3) Slowly adding 95% ethanol into the concentrated extract, stirring until the ethanol concentration of the mixed solution is 75%, standing at a temperature lower than 10deg.C for 12 hr, filtering the supernatant, collecting filtrate, and discarding the precipitate to obtain filtrate B;
(4) Weighing 150g of boric acid, 25g of borax and 25g of chlorhexidine acetate, respectively adding 450g of purified water, 75g of purified water and 75g of purified water, stirring until the purified water is fully dissolved, mixing the two components together, and filtering to obtain a mixed solution;
(5) Mixing the filtrate B with the mixed solution, adding purified water until the total amount reaches 6.1kg, stirring uniformly, adjusting the pH value to 6 with hydrochloric acid, and stirring for 30min to obtain the honeysuckle antibacterial solution.
Example 3
The honeysuckle antibacterial liquid is prepared from the following raw materials in parts by weight as shown in table 1:
(1) Weighing 50g of honeysuckle, 20g of fructus cnidii, 35g of radix sophorae flavescentis and 12g of pepper according to the weight, wrapping the fructus cnidii with cotton cloth, mixing the wrapped fructus cnidii with other medicinal materials, extracting for one time, adding 877g of water, heating to keep micro-boiling, extracting and refluxing for 1.5 hours, and filtering the liquid medicine through a 200-mesh screen; adding 877g of water into the filter residue for secondary extraction, repeating the primary extraction steps, and combining the filtrates obtained by the secondary extraction to obtain filtrate A;
(2) Vacuum concentrating the filtrate A, controlling vacuum concentration vacuum degree to-0.05 MPa, concentrating at 65deg.C until the relative density is 1.10, and standing at normal temperature to obtain concentrated extract;
(3) Slowly adding 95% ethanol into the concentrated extract, stirring until the ethanol concentration of the mixed solution is 75%, standing at a temperature lower than 10deg.C for 11.5 hr, filtering the supernatant, collecting filtrate, and discarding the precipitate to obtain filtrate B;
(4) Weighing 160g of boric acid, 30g of borax and 30g of chlorhexidine acetate, respectively adding 320g of purified water, 60g of purified water and 60g of purified water, stirring until the purified water is fully dissolved, mixing the two components together, and filtering to obtain a mixed solution;
(5) Mixing the filtrate B with the mixed solution, adding purified water until the total amount reaches 7.1kg, stirring uniformly, adjusting the pH value to 7 with hydrochloric acid, and stirring for 30min to obtain the honeysuckle antibacterial solution.
Example 4
The honeysuckle antibacterial liquid is different from the embodiment 2 in that 8g of citric acid higher fatty alcohol ester is added in the embodiment, and the citric acid higher fatty alcohol ester added in the embodiment is stearyl citrate, and the preparation method comprises the following steps:
(1) Weighing 30g of honeysuckle, 30g of fructus cnidii, 30g of radix sophorae flavescentis and 15g of pepper according to the weight, wrapping the fructus cnidii with cotton cloth, mixing the wrapped fructus cnidii with other medicinal materials, extracting and adding 840g of water for one time, heating and keeping micro-boiling, extracting and refluxing for 1 hour, and filtering the liquid medicine through a 200-mesh screen; adding 840g of water into filter residues in the secondary extraction, repeating the primary extraction step, and combining the filtrates of the two extractions to obtain filtrate A;
(2) Vacuum concentrating the filtrate A, controlling vacuum concentration degree to-0.06 MPa, concentrating at 70deg.C until the relative density is 1.15, and standing at normal temperature to obtain concentrated extract;
(3) Slowly adding 95% ethanol into the concentrated extract, stirring until the ethanol concentration of the mixed solution is 75%, standing at a temperature lower than 10deg.C for 12 hr, filtering the supernatant, collecting filtrate, and discarding the precipitate to obtain filtrate B;
(4) Weighing 150g of boric acid, 25g of borax and 25g of chlorhexidine acetate, respectively adding 450g of purified water, 75g of purified water and 75g of purified water, stirring until the purified water is fully dissolved, mixing the two components together, and filtering to obtain a mixed solution;
(5) Mixing the filtrate B with the mixed solution, adding 8g of stearyl citrate, adding purified water until the total amount reaches 6.1kg, stirring uniformly, regulating the pH value to 6 by using hydrochloric acid, and stirring for 30min to obtain the honeysuckle antibacterial solution.
Example 5
A honeysuckle antibacterial liquid is different from example 4 in that 12g of stearyl citrate is added in the example.
Example 6
A honeysuckle antibacterial liquid is different from example 4 in that 10g of stearyl citrate is added in the example.
Example 7
A honeysuckle antibacterial liquid is different from example 6 in that 10g of citric acid higher fatty alcohol ester added in this example is fatty alcohol polyethylene citrate prepared in preparation example 1.
Example 8
A honeysuckle antibacterial liquid is different from example 6 in that 10g of citric acid higher fatty alcohol ester added in this example is fatty alcohol polyethylene citrate prepared in preparation example 2.
Example 9
The honeysuckle antibacterial liquid is different from the embodiment 8 in that 10g of phenyllactic acid is also added in the embodiment.
Example 10
A honeysuckle antibacterial liquid is different from example 8 in that 15g of phenyllactic acid is also added in the example.
Example 10
The honeysuckle antibacterial liquid is different from the embodiment 8 in that 13g of phenyllactic acid is also added in the embodiment.
Comparative example
Comparative example 1
The antibacterial solution for honeysuckle flower is different from the antibacterial solution for honeysuckle flower in the embodiment 2 in that no honeysuckle flower is added.
Comparative example 2
A honeysuckle antibacterial liquid is different from the embodiment 2 in that fructus cnidii is not added in the embodiment.
Comparative example 3
A honeysuckle antibacterial liquid is different from the embodiment 2 in that no radix sophorae flavescentis is added in the embodiment.
Comparative example 4
A honeysuckle antibacterial liquid is different from the embodiment 2 in that no pricklyash peel is added in the embodiment.
Comparative example 5
A honeysuckle antibacterial liquid is different from the embodiment 10 in that boric acid is not added in the embodiment.
Comparative example 6
A honeysuckle antibacterial liquid is different from the embodiment 10 in that borax is not added in the embodiment.
Comparative example 7
A honeysuckle antibacterial liquid is different from the embodiment 10 in that chlorhexidine acetate is not added in the embodiment.
Performance test
Detection method
Antibacterial activity assay: the bacteriostasis rate of the product was tested according to GB15979-2002 appendix C.
Potato (PDA) medium: 200g of potato, 20g of glucose, 20g of agar and 1000mL of sterilized water. Peeling 200g potato, cutting into small pieces, boiling in 1000mL water for 30min, filtering with four layers of gauze, collecting filtrate, adding glucose and agar, adding sterilized water to 1000mL, and sterilizing at 121deg.C for 20min.
Beef extract peptone medium: 10g of beef extract, 5g of peptone, 5g of sodium chloride, 20g of agar and 1000mL of sterilizing water are taken, the pH is adjusted to 7.4, and the beef extract is sterilized at 121 ℃ for 20min.
Preparation of a bacterial suspension of a test strain: (1) Taking out staphylococcus aureus and candida albicans from a refrigerator at 4 ℃, inoculating the staphylococcus aureus and candida albicans into a PDA culture medium, culturing at a constant temperature of 37 ℃ for 24 hours for activation, and culturing the candida albicans at a constant temperature of 28 ℃ for 48 hours for activation. Transferring 1.0ml of the stock solution into 9.0ml of sterile water to obtain 10-fold diluted suspension, and sequentially operating until the dilution is 10 -3 And (5) standby. (2) Inoculating penicillium and aspergillus niger to beef extract peptone culture medium for activation, washing spores with a certain amount of sterile water, preparing 110cfu/mL fungus suspension by turbidimetry, and reserving at 4 ℃.
4 parts of 5ml of the antibacterial solutions prepared in examples 1-11 and comparative examples 1-7 are respectively taken and labeled in a sterile operation, 100 mu L of bacterial suspensions of all strains are respectively taken, and after the bacterial suspensions are respectively added for 5min, the antibacterial rate of each sample is tested.
Table 1 antibacterial effect test results of honeysuckle antibacterial liquid
| Staphylococcus aureus (%) | Candida albicans (%) | Penicillium (%) | Aspergillus niger (%) | |
| Example 1 | 98.15 | 98.23 | 96.44 | 98.11 |
| Example 2 | 98.29 | 98.41 | 96.81 | 98.45 |
| Example 3 | 98.19 | 98.35 | 96.74 | 98.29 |
| Example 4 | 98.61 | 98.76 | 98.11 | 98.65 |
| Example 5 | 98.7 | 98.81 | 98.19 | 98.71 |
| Example 6 | 98.91 | 99.01 | 98.36 | 98.79 |
| Example 7 | 99.52 | 99.62 | 98.47 | 99.68 |
| Example 8 | 99.63 | 99.74 | 98.59 | 99.71 |
| Example 9 | 99.91 | 99.94 | 99.96 | 99.95 |
| Example 10 | 99.96 | 99.96 | 99.98 | 99.97 |
| Example 11 | 99.99 | 99.99 | 99.99 | 99.99 |
| Comparative example 1 | 94.21 | 95.16 | 93.69 | 94.55 |
| Comparative example 2 | 95.64 | 96.19 | 94.32 | 95.26 |
| Comparative example 3 | 94.28 | 95.69 | 93.88 | 94.78 |
| Comparative example 4 | 96.75 | 97.11 | 95.56 | 96.17 |
| Comparative example 5 | 96.21 | 97.05 | 95.99 | 96.85 |
| Comparative example 6 | 96.15 | 97.09 | 95.87 | 96.71 |
| Comparative example 7 | 93.01 | 92.89 | 93.19 | 94.02 |
180 patients with itching and inflammation of ears are randomly selected in the experiment, the attack is aggravated more than february in the course of disease and more than 2 weeks, and 10 persons are randomly grouped into an example group and a comparative example group. Through statistical tests, the two groups of patients have no obvious difference in sex, age, disease course, disease parts and symptom severity, and have comparability, and all patients use other antibacterial medicines within 1 month. After the experiment is started, the antibacterial liquid prepared by each example and comparative example in the application is dripped into an affected part, and is soaked for 5 minutes at least with the affected part for 2 times per day, the condition of the affected part is observed every day, the experiment is ended after 7 days, the symptoms such as inflammation and the like of the affected part are observed to be disappeared, particularly, the symptoms of the patients using the antibacterial liquid of examples 9-11 are obviously improved at the end of the first day, the patients in examples 4-8 had substantially healed on day four, examples 1-3 had substantially healed on day five, and the remaining comparative inflammatory symptoms had reduced healing on day six and seven, with slightly reddish ears in the patients using the antibacterial fluid of comparative example 7. Invitees scored as follows: degree of itching: 0 = none, 1 = mild itching, 2 = overt itching, 3 = severe itching; stimulation feel: 0=mild no stimulus, 1=mild stimulus, 2=uncomfortable, 3=strongly uncomfortable. Statistical results show that all the examples 1-11 can solve the problem of ear itching of people with ear inflammation, and the examples 1-3, which are not added with citric acid higher fatty alcohol ester, are scored slightly stimulated, the comparative examples 5-6 are uncomfortable and the foreign body sensation is heavy. For the antibacterial liquid added with the citric acid higher fatty alcohol ester, particularly the fatty alcohol polyethylene citrate, the reaction of the mode is mild and comfortable when the antibacterial liquid is dripped, the ear feeling is good, and the ear skin of a patient using the antibacterial liquid of the groups of examples 4-11, particularly the antibacterial liquid of the groups of 7-11 is observed to be lustrous and finer than the ear skin of the patient using the antibacterial liquid of the groups of examples 1-3.
As can be seen by combining examples 1-11 and comparative examples 1-7 and combining Table 1, the inhibition rates of examples 1-11 on various bacteria are better than those of comparative examples 1-7, so that good synergistic interaction among the components of the honeysuckle antibacterial liquid disclosed by the application achieves better antibacterial effect on common bacteria of ears.
When the honeysuckle, the cnidium fruit, the kuh-seng and the pricklyash peel are not added in comparative examples 1-4 respectively, the antibacterial effect is reduced to some extent, the antibacterial effect is not optimal, and the fact that several effective components in the application need to be combined together to have a synergistic effect is indicated to have a more remarkable weight-losing effect.
It can be seen from the combination of example 2 and comparative examples 5 to 6 and the combination of table 1 that the inhibition rate of example 2 against various bacteria is superior to that of comparative examples 5 to 6, which means that when boric acid or borax is added alone, the optimal antibacterial effect cannot be achieved, and at the same time, discomfort and foreign body sensation are caused in the reaction when the treatment effect evaluation is performed, which means that the combined addition of borax and boric acid enhances the antibacterial effect on the one hand, and on the other hand, the antibacterial liquid is milder and does not generate strong irritation.
As can be seen from the combination of example 2, example 10 and comparative example 7 and the combination of table 1, the antibacterial efficiency of comparative example 7 is greatly reduced when chlorhexidine acetate is not added, which indicates that the addition of chlorhexidine acetate can not only form good adaptation and synergy with the traditional Chinese medicine components, but also generate good synergistic antibacterial effect with the added citric acid higher fatty alcohol ester and phenyllactic acid.
It can be seen from the combination of the embodiment 2 and the embodiments 4-11 that when the proper amount of the citric acid higher fatty alcohol ester and the phenyllactic acid are added, the antibacterial ability of the antibacterial liquid is improved, which indicates that the added citric acid higher fatty alcohol ester and the phenyllactic acid can be well matched with other components in the antibacterial liquid, and the antibacterial effect is enhanced.
The present embodiment is merely illustrative of the present application and is not intended to be limiting, and those skilled in the art, after having read the present specification, may make modifications to the present embodiment without creative contribution as required, but is protected by patent laws within the scope of the claims of the present application.
Claims (4)
1. The honeysuckle antibacterial liquid is characterized by comprising the following raw materials in parts by weight: 20-50 parts of honeysuckle, 20-40 parts of fructus cnidii, 15-35 parts of radix sophorae flavescentis, 12-18 parts of pepper, 140-160 parts of boric acid, 20-30 parts of borax, 20-30 parts of chlorhexidine acetate and 8-12 parts of citric acid higher fatty alcohol ester; the citric acid higher fatty alcohol ester is fatty alcohol polyethylene citric acid ester; the fatty alcohol polyethylene citrate in the raw materials is prepared by the following steps: (1) citric acid and fatty alcohol polyoxyethylene ether in a proportion of 1:1.5-2.0, adding a catalyst, controlling the reaction temperature within 140-160 ℃ and fully reacting; (2) After the reaction is finished, decompressing and rotary evaporating, and fully removing water to obtain the fatty alcohol polyethylene citrate; the preparation method of the honeysuckle antibacterial liquid comprises the following operation steps:
(1) Weighing the raw materials of the traditional Chinese medicine components according to the weight, mixing together, wrapping the fructus cnidii with cotton cloth, extracting for one time, adding 7-8 times of water relative to the traditional Chinese medicine materials, heating to keep micro boiling, extracting and refluxing for 0.8-1.5h, and filtering by a screen; adding water into the filter residue, repeating the steps, and combining the filtrates of the two extractions to obtain filtrate A;
(2) Concentrating the filtrate A under vacuum, controlling vacuum concentration at a vacuum degree of not less than-0.07 MPa and 65-75deg.C until the relative density is 1.10-1.20, and standing at normal temperature to obtain concentrated extract;
(3) Slowly adding ethanol into the concentrated extract, stirring while controlling the temperature to be less than or equal to 10 ℃, standing for 11-12h, filtering the supernatant, collecting filtrate, and discarding precipitate to obtain filtrate B;
(4) Weighing corresponding amounts of boric acid, borax and chlorhexidine acetate, respectively adding 2-3 times of purified water, stirring to dissolve completely, mixing together, and filtering to obtain mixed solution;
(5) Mixing the filtrate B with the mixed solution, adding fatty alcohol polyethylene citrate, adding purified water to 19-21 times of the raw materials and auxiliary materials, stirring uniformly, regulating the pH value to 5-7 with hydrochloric acid, and stirring uniformly to obtain the honeysuckle antibacterial solution.
2. A honeysuckle antibacterial liquid is characterized in that: the material consists of the following raw materials in parts by weight: 20-50 parts of honeysuckle, 20-40 parts of fructus cnidii, 15-35 parts of radix sophorae flavescentis, 12-18 parts of pepper, 140-160 parts of boric acid, 20-30 parts of borax, 20-30 parts of chlorhexidine acetate, 8-12 parts of citric acid higher fatty alcohol ester and 10-15 parts of phenyllactic acid; the citric acid higher fatty alcohol ester is fatty alcohol polyethylene citric acid ester; the fatty alcohol polyethylene citrate in the raw materials is prepared by the following steps: (1) citric acid and fatty alcohol polyoxyethylene ether in a proportion of 1:1.5-2.0, adding a catalyst, controlling the reaction temperature within 140-160 ℃ and fully reacting; (2) After the reaction is finished, decompressing and rotary evaporating, and fully removing water to obtain the fatty alcohol polyethylene citrate; the preparation method of the honeysuckle antibacterial liquid comprises the following operation steps:
(1) Weighing the raw materials of the traditional Chinese medicine components according to the weight, mixing together, wrapping the fructus cnidii with cotton cloth, extracting for one time, adding 7-8 times of water relative to the traditional Chinese medicine materials, heating to keep micro boiling, extracting and refluxing for 0.8-1.5h, and filtering by a screen; adding water into the filter residue, repeating the steps, and combining the filtrates of the two extractions to obtain filtrate A;
(2) Concentrating the filtrate A under vacuum, controlling vacuum concentration at a vacuum degree of not less than-0.07 MPa and 65-75deg.C until the relative density is 1.10-1.20, and standing at normal temperature to obtain concentrated extract;
(3) Slowly adding ethanol into the concentrated extract, stirring while controlling the temperature to be less than or equal to 10 ℃, standing for 11-12h, filtering the supernatant, collecting filtrate, and discarding precipitate to obtain filtrate B;
(4) Weighing corresponding amounts of boric acid, borax and chlorhexidine acetate, respectively adding 2-3 times of purified water, stirring to dissolve completely, mixing together, and filtering to obtain mixed solution;
(5) Mixing the filtrate B with the mixed solution, adding fatty alcohol polyethylene citrate and phenyllactic acid, adding purified water until the amount of raw materials is 19-21 times of that of the auxiliary materials, stirring uniformly, regulating the pH value to be 5-7 by using hydrochloric acid, and stirring uniformly to obtain the honeysuckle antibacterial solution.
3. The honeysuckle antibacterial liquid according to claim 2, wherein the honeysuckle antibacterial liquid is prepared from the following raw materials in parts by weight: 25-35 parts of honeysuckle, 25-35 parts of fructus cnidii, 25-30 parts of radix sophorae flavescentis, 13-16 parts of pepper, 145-155 parts of boric acid, 22-26 parts of borax, 22-26 parts of chlorhexidine acetate, 12-14 parts of phenyllactic acid and 9-11 parts of fatty alcohol polyethylene citrate.
4. The antibacterial honeysuckle flower liquid according to claim 1, wherein 95% ethanol is added in the step (3), and the adding is stopped when the ethanol concentration of the mixed liquid is 75% while stirring.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000143437A (en) * | 1998-11-09 | 2000-05-23 | Ichimaru Pharcos Co Ltd | Cosmetic composition containing huhectant vegetable extract |
| CN101007073A (en) * | 2007-01-16 | 2007-08-01 | 刘二伟 | Traditional Chinese medicine lotus lotion and its preparation method |
| CN111298059A (en) * | 2020-03-18 | 2020-06-19 | 湖南宝护者生物技术有限公司 | Skin mucosa disinfectant prepared from Chinese herbal medicines and preparation method thereof |
| CN111821349A (en) * | 2020-07-14 | 2020-10-27 | 郑州维谊生物科技有限公司 | Foot skin antibacterial liquid and preparation method thereof |
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2023
- 2023-01-29 CN CN202310044135.7A patent/CN116115683B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2000143437A (en) * | 1998-11-09 | 2000-05-23 | Ichimaru Pharcos Co Ltd | Cosmetic composition containing huhectant vegetable extract |
| CN101007073A (en) * | 2007-01-16 | 2007-08-01 | 刘二伟 | Traditional Chinese medicine lotus lotion and its preparation method |
| CN111298059A (en) * | 2020-03-18 | 2020-06-19 | 湖南宝护者生物技术有限公司 | Skin mucosa disinfectant prepared from Chinese herbal medicines and preparation method thereof |
| CN111821349A (en) * | 2020-07-14 | 2020-10-27 | 郑州维谊生物科技有限公司 | Foot skin antibacterial liquid and preparation method thereof |
Non-Patent Citations (1)
| Title |
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| 射干、金银花等八种中药抗真菌实验研究;于军;苏学今;王丽;;军医进修学院学报(第04期);299-300 * |
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