CN116076502A - Insecticidal mildew-proof tobacco mellowing composition, and preparation method and application thereof - Google Patents
Insecticidal mildew-proof tobacco mellowing composition, and preparation method and application thereof Download PDFInfo
- Publication number
- CN116076502A CN116076502A CN202310217451.XA CN202310217451A CN116076502A CN 116076502 A CN116076502 A CN 116076502A CN 202310217451 A CN202310217451 A CN 202310217451A CN 116076502 A CN116076502 A CN 116076502A
- Authority
- CN
- China
- Prior art keywords
- mixture
- mixing
- tobacco
- mildew
- composition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/20—Biochemical treatment
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/06—Unsaturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/04—Insecticides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention relates to a tobacco mellowing composition with insecticidal and mildew-proof functions, and a preparation method and application thereof, and belongs to the technical field of tobacco processing. The invention provides an insecticidal mildew-proof tobacco mellowing composition, which comprises glucose oxidase, catalase, glucose, mildew inhibitor, chitosan, glycerol, acetic acid and water. The tobacco mellowing composition provided by the invention is composed of natural components, is safe and nontoxic to use, can form a layer of film when being sprayed on tobacco, has mechanical property and barrier property, has a moisturizing effect, can keep the moisture content of tobacco in the alcoholizing process, is more beneficial to natural alcoholization of the tobacco, and can consume oxygen by glucose oxidase and catalase in the film, and can achieve the effects of insect resistance and mildew resistance on the naturally alcoholized tobacco by controlling the content of oxygen and inhibiting the growth of mould when the mildew preventive interacts with each other.
Description
Technical Field
The invention relates to a tobacco mellowing composition with insecticidal and mildew-proof functions, and a preparation method and application thereof, and belongs to the technical field of tobacco processing.
Background
Before tobacco leaves enter a cigarette production link, the tobacco leaves subjected to primary threshing and redrying need to be stored for a period of time for natural alcoholization, and the period is generally 1-3 years; in the natural alcoholization process, the tobacco leaves can generate a series of chemical changes, especially under the synergistic effect of microorganisms, the green and miscellaneous taste and the irritation of the tobacco leaves can be reduced, the fragrance is increased, the odor absorption is mellow, and the color is more uniform; natural alcoholization of tobacco leaves can improve the aroma and the taste quality of the tobacco leaves; however, tobacco leaves are rich in nutrients such as sugar, nicotine and protein, insects are easy to generate when the tobacco leaves are closely contacted with the external environment for a long time, and the tobacco leaves are easy to generate mildew when the temperature is too high and the humidity is too high in the natural alcoholization process; the quality of the tobacco leaves is seriously affected by insects or mildewed tobacco leaves, loss is caused to cigarette manufacturers and national tax, and toxin generated by mildewing directly endangers the health of consumers.
In solving the problem of tobacco leaf insect growth, the integrated control of phosphine gas fumigation is generally adopted in the past, or a sealed ultralow oxygen storage method is adopted, and the oxygen in the storage environment is controlled below 2% to quickly inhibit and kill microorganisms and plant diseases and insect pests in the storage environment; the phosphine fumigation is adopted to kill insects, although the effect is remarkable, the phosphine is extremely toxic, inflammable and explosive, personnel poisoning and other malignant safety accidents are easy to cause in the fumigation insecticidal process, meanwhile, certain harm is brought to the surrounding environment and people and livestock, in addition, the nontoxic treatment of the fumigated waste gas, waste liquid and waste residue is troublesome, and the fumigation is forbidden at present; the oxygen concentration in the tobacco leaf storage environment is reduced to below 10% by adopting a sealing ultralow oxygen storage method to perform oxygen reduction, disinsection and mildew prevention, namely, natural oxygen reduction, microbial oxygen reduction, chemical agent oxygen reduction or physical air conditioning oxygen reduction and the like, and the tobacco leaf storage environment is kept for a period of time to perform disinsection; natural oxygen reduction and microbial oxygen reduction effects are poor, and the oxygen reduction speed is low; the chemical agent is used for reducing oxygen, such as an iron reducing agent, so that the oxygen reduction speed is too high, the oxygen concentration can be quickly reduced to below 2%, although the chemical agent can be used for quickly killing insects, the tobacco leaves are in a low-oxygen environment below 2% for a long time, the alcoholization speed of the tobacco leaves is excessively reduced, the color of the tobacco leaves is easy to be darkened, and the usability is reduced; if the physical air conditioning oxygen reduction is carried out by introducing gases such as nitrogen, ozone and carbon dioxide for replacement, the investment of air making equipment can exist, the production cost of enterprises is increased, meanwhile, the operation is unchanged, and the risk of gas leakage exists.
In order to solve the problem of mildew of tobacco leaves, temperature and humidity of a storehouse are generally regulated by installing temperature and humidity regulating equipment in the past to control the mildew, or the temperature and humidity of the storehouse are regulated by adopting a natural ventilation or calcium chloride dehumidification mode, so that the moisture absorption and mildew of the tobacco leaves are controlled; the temperature and humidity of the storeroom are adjusted by adopting the temperature and humidity adjusting equipment, or the temperature and humidity of the storeroom is adjusted by adopting the modes of natural ventilation or calcium chloride dehumidification and the like, so that the problem of tobacco mildew cannot be fundamentally solved.
On the basis of low cost, energy conservation and consumption reduction, the invention can effectively control the insect mildew of the tobacco leaves in the natural alcoholization period of the tobacco leaves.
Disclosure of Invention
In order to solve the problems, the invention provides a tobacco alcoholization composition for killing insects and preventing mildew, which comprises glucose oxidase, catalase, glucose, a mildew inhibitor, chitosan, glycerol, acetic acid and water.
In one embodiment of the invention, the ingredients of the tobacco mellowing composition comprise, by weight, 0.0016 to 0.0065 parts of glucose oxidase, 0.0016 to 0.0065 parts of catalase, 0.033 to 0.33 parts of glucose, 0.0016 to 0.0065 parts of mildew inhibitor, 0.882 parts of chitosan, 0.156 parts of glycerol, 0.0873 parts of acetic acid and 98.5252 to 98.8369 parts of water.
In one embodiment of the present invention, the component of the mildew preventive comprises one or a combination of more than one of dimethyl fumarate, sodium diacetate and calcium propionate.
In one embodiment of the invention, the glucose oxidase is food grade glucose oxidase, and the enzyme activity is more than or equal to 10000U/g.
In one embodiment of the invention, the catalase is food grade catalase, and the enzyme activity is more than or equal to 100000U/g.
In one embodiment of the invention, the chitosan is technical grade chitosan.
In one embodiment of the invention, the chitosan has a deacetylation of 95% or more.
In one embodiment of the invention, the glycerol is food grade glycerol.
In one embodiment of the invention, the acetic acid is greater than or equal to 98% pure.
The invention also provides a method for preparing the insecticidal and mildew-proof tobacco alcoholization composition, which comprises the steps of adding a glycerol aqueous solution into a mixed aqueous solution of glucose oxidase, catalase, glucose, acetic acid, a mildew inhibitor and chitosan, and mixing to obtain the insecticidal and mildew-proof tobacco alcoholization composition.
In one embodiment of the invention, the method comprises the steps of:
step one: adding glucose oxidase and catalase into water, and mixing to obtain a mixture A;
step two: adding glucose into water, and mixing to obtain a mixture B;
step three: adding acetic acid into water, and mixing to obtain a mixture C;
step four: adding glycerol into water, and mixing to obtain a mixture D;
step five: adding a mildew preventive into water, and mixing to obtain a mixture E;
step six: adding chitosan into the mixture C, and mixing to obtain a mixture F;
step seven: mixing the mixture A, the mixture B and the mixture E to obtain a mixture G;
step eight: adding the mixture G into the mixture F, and mixing to obtain a mixture H;
step nine: and adding the mixture D into the mixture H, and mixing to obtain the insecticidal and mildew-proof tobacco mellowing composition.
In one embodiment of the present invention, the first step is: 0.05-0.2 g of glucose oxidase and 0.05-0.2 g of catalase are added into 100mL of water, and the mixture A is obtained by mixing.
In one embodiment of the present invention, in the first step, the method further comprises aseptic filtration using a filter membrane having a pore size of 0.22 μm after mixing glucose oxidase, catalase and water.
In one embodiment of the present invention, the second step is: 1-10 g of glucose is added into 90-99 mL of water and mixed to obtain a mixture B.
In one embodiment of the present invention, in the second step, the method further comprises aseptic filtration using a filter membrane having a pore size of 0.22 μm after mixing glucose and water.
In one embodiment of the present invention, the third step is: 1mL of acetic acid was added to 99mL of water and mixed to give mixture C.
In one embodiment of the present invention, the fourth step is: 6mL of glycerol was added to 94mL of water and mixed to give mixture D.
In one embodiment of the present invention, the fifth step is: adding 0.05-0.2 g of mildew inhibitor into 100mL of water, and mixing to obtain a mixture E.
In one embodiment of the present invention, the sixth step is: 1g of chitosan was added to 99mL of the mixture C, and mixed to obtain a mixture F.
In one embodiment of the present invention, the step seven is: the mixture A, the mixture B and the mixture E were mixed in a volume ratio of 1:1:1 to obtain a mixture G.
In one embodiment of the present invention, the step eight is: 10mL of mixture G was added to 90mL of mixture F and mixed to give mixture H.
In one embodiment of the present invention, the step nine is: and adding the mixture D into the mixture H at a rate of 1-2 mL/min, and mixing to obtain the insecticidal mildew-proof tobacco mellowing composition.
In one embodiment of the present invention, the rate in step nine is 2mL/min.
The invention also provides the application of the insecticidal mildew-proof tobacco mellowing composition or the method for preparing the insecticidal mildew-proof tobacco mellowing composition in tobacco mellowing.
The technical scheme of the invention has the following advantages:
1. the invention provides an insecticidal mildew-proof tobacco mellowing composition, which comprises glucose oxidase, catalase, glucose, mildew inhibitor, chitosan, glycerol, acetic acid and water. The tobacco mellowing composition adopts biological enzyme method oxygen reduction technology, and glucose oxidase and catalase form an oxidation-reduction enzyme system; wherein the glucose oxidase is capable of oxidizing glucose in the presence of molecular oxygen to produce D-gluconolactone while consuming oxygen to produce hydrogen peroxide, and the catalase is capable of decomposing hydrogen peroxide to produce water and 1/2 of the oxygen to consume oxygen; the biological enzyme deoxidizing method is quick and effective, has outstanding advantages of energy conservation, emission reduction and synergy, can control oxygen within a certain concentration range (6-12 percent), does not influence the natural alcoholization of tobacco leaves, and has the advantages of simple and convenient operation, environmental protection and the like.
2. In the tobacco mellowing composition of the invention, optional mildew preventive comprises dimethyl fumarate, sodium diacetate and calcium propionate; the sodium diacetate and calcium propionate mildew preventive are novel food and feed mildew preventive with stable property and low price, and have good inhibition capability on mildew; the dimethyl fumarate is widely used for preventing corrosion, mildew and fresh-keeping of foods, grains, feeds, tobacco, leather, clothes and the like, has the characteristics of low toxicity, high efficiency and broad-spectrum antibacterial property, has special antibacterial effect on mold, has insecticidal activity, and also has touch-killing and fumigating effects.
3. In the natural alcoholization process of tobacco leaves, especially when the natural alcoholization time is longer, the tobacco leaves are fragile due to the reduced moisture retention capacity of the tobacco leaves, and the tobacco leaves are easy to break in the subsequent processing; therefore, maintaining proper moisture in the tobacco leaves is particularly important in the tobacco mellowing process; the chitosan in the tobacco mellowing composition is a chitosan deacetylated product, and is a linear polymer formed by connecting 2-acetamido-2-deoxy-D-glucopyranose through beta-D- (1-4) glycosidic bond, and has the advantages of good biocompatibility, degradability, regenerability, antibacterial and antiseptic properties, film forming properties and the like; due to the film forming property of chitosan, a layer of chitosan film is formed after the chitosan film is sprayed on tobacco leaves, and the chitosan film has certain mechanical property and barrier property, has a moisturizing effect, can well maintain the moisture content of the tobacco leaves in the alcoholization process, and is more beneficial to natural alcoholization of the tobacco leaves; meanwhile, the anti-insect and anti-mildew agent also has a blocking effect on mould spores and oxygen in the air, and has an anti-insect and anti-mildew effect.
4. The invention can effectively avoid the phenomena of reduced availability of tobacco leaves and the like caused by excessively reducing the alcoholization speed of the tobacco leaves due to overlong hypoxia sealing time.
5. The invention has the advantages of easily available raw materials, simple process, obvious effect, lower cost and easy popularization; in addition, the invention adopts the raw materials with high safety and low toxicity, and does not generate toxic and harmful substances in the insecticidal mildew-proof process of the stored tobacco leaves, so that the tobacco leaves are not polluted in the insecticidal process, the environmental pollution is not caused, and the invention meets the requirements of clean production.
Detailed Description
The following examples are provided for a better understanding of the present invention and are not limited to the preferred embodiments described herein, but are not intended to limit the scope of the invention, any product which is the same or similar to the present invention, whether in light of the present teachings or in combination with other prior art features, falls within the scope of the present invention.
The following examples do not identify specific experimental procedures or conditions, which may be followed by procedures or conditions of conventional experimental procedures described in the literature in this field. The reagents or apparatus used were conventional reagent products commercially available without the manufacturer's knowledge.
The glucose oxidase referred in the following examples was purchased from the food additives Limited company, bai Cia Zhengzhou, and the enzyme activity was not less than 10000U/g; catalase is purchased from Zhengzhou Baishite food additive Co., ltd, and the enzyme activity is more than or equal to 100000U/g; glucose is purchased from Henan Huiteng chemical product sales company, and has food grade and purity of more than or equal to 99%; dimethyl fumarate is purchased from Shanghai Michelia Biochemical technology Co., ltd, and the purity is more than or equal to 99%; sodium diacetate is purchased from Henan Chengxiang food additive Co., ltd, and has a food grade and a purity of not less than 99%; calcium propionate is purchased from Zhengzhou Baishite food additive Co., ltd, food grade, purity is more than or equal to 99%; the chitosan is purchased from Zhengzhou Baishite food additive Co., ltd, the purity is more than or equal to 99%, and the deacetylation is more than or equal to 95%; the glycerol is purchased from Henan Yoghurt chemical products limited company, and the content of the glycerol is more than or equal to 99 percent; acetic acid is purchased from Henan Jie chemical products Co., ltd, food grade, and content is more than or equal to 98%.
Example 1-1: insecticidal mildew-proof tobacco mellowing composition and preparation method thereof
The embodiment provides a tobacco mellowing composition for killing insects and preventing mildew, wherein the components of the tobacco mellowing composition comprise glucose oxidase, catalase, glucose, mildew preventive (dimethyl fumarate, sodium diacetate and calcium propionate), chitosan, glycerol and acetic acid.
The preparation method of the insecticidal mildew-proof tobacco mellowing composition comprises the following steps:
step one: adding 0.05g of glucose oxidase and 0.05g of catalase into 100mL of distilled water, mixing for 2min under magnetic stirring at a rotation speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture A;
step two: adding 1g of glucose into 99mL of distilled water, mixing for 2min under magnetic stirring at a rotating speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture B;
step three: adding 1mL of acetic acid into 99mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture C;
step four: adding 6mL of glycerol into 94mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture D;
step five: 0.05g of dimethyl fumarate, 0.05g of sodium diacetate and 0.05g of calcium propionate were added to 100mL of distilled water, and mixed for 2min under magnetic stirring at 150rpm to obtain a mixture E;
step six: 1g of chitosan is added into 99mL of the mixture C, and the mixture C is mixed for 30min under the magnetic stirring with the rotating speed of 150rpm, so as to obtain a mixture F;
step seven: mixing the mixture A, the mixture B and the mixture E in a volume ratio of 1:1:1, and mixing for 5min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture G;
step eight: adding 10mL of the mixture G into 90mL of the mixture F, and mixing for 30min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture H;
step nine: 2mL of the mixture D was added to 98mL of the mixture H at a rate of 2mL/min, and the mixture was mixed for 30min under magnetic stirring at a rotation speed of 150rpm, to obtain a pesticidal and antifungal tobacco mellowing composition.
Examples 1-2: insecticidal mildew-proof tobacco mellowing composition and preparation method thereof
The embodiment provides a tobacco mellowing composition for killing insects and preventing mildew, wherein the components of the tobacco mellowing composition comprise glucose oxidase, catalase, glucose, mildew preventive (dimethyl fumarate, sodium diacetate and calcium propionate), chitosan, glycerol and acetic acid.
The preparation method of the insecticidal mildew-proof tobacco mellowing composition comprises the following steps:
step one: adding 0.1g of glucose oxidase and 0.1g of catalase into 100mL of distilled water, mixing for 2min under magnetic stirring at a rotation speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture A;
step two: adding 3g of glucose into 97mL of distilled water, mixing for 2min under magnetic stirring at a rotating speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture B;
step three: adding 1mL of acetic acid into 99mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture C;
step four: adding 6mL of glycerol into 94mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture D;
step five: 0.05g of dimethyl fumarate, 0.05g of sodium diacetate and 0.05g of calcium propionate were added to 100mL of distilled water, and mixed for 2min under magnetic stirring at 150rpm to obtain a mixture E;
step six: 1g of chitosan is added into 99mL of the mixture C, and the mixture C is mixed for 30min under the magnetic stirring with the rotating speed of 150rpm, so as to obtain a mixture F;
step seven: mixing the mixture A, the mixture B and the mixture E in a volume ratio of 1:1:1, and mixing for 5min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture G;
step eight: adding 10mL of the mixture G into 90mL of the mixture F, and mixing for 30min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture H;
step nine: 2mL of the mixture D was added to 98mL of the mixture H at a rate of 2mL/min, and the mixture was mixed for 30min under magnetic stirring at a rotation speed of 150rpm, to obtain a pesticidal and antifungal tobacco mellowing composition.
Examples 1-3: insecticidal mildew-proof tobacco mellowing composition and preparation method thereof
The embodiment provides a tobacco mellowing composition for killing insects and preventing mildew, wherein the components of the tobacco mellowing composition comprise glucose oxidase, catalase, glucose, mildew preventive (dimethyl fumarate, sodium diacetate and calcium propionate), chitosan, glycerol and acetic acid.
The preparation method of the insecticidal mildew-proof tobacco mellowing composition comprises the following steps:
step one: adding 0.15g of glucose oxidase and 0.15g of catalase into 100mL of distilled water, mixing for 2min under magnetic stirring at a rotation speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture A;
step two: adding 6g of glucose into 94mL of distilled water, mixing for 2min under magnetic stirring at a rotation speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture B;
step three: adding 1mL of acetic acid into 99mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture C;
step four: adding 6mL of glycerol into 94mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture D;
step five: 0.05g of dimethyl fumarate, 0.05g of sodium diacetate and 0.05g of calcium propionate were added to 100mL of distilled water, and mixed for 2min under magnetic stirring at 150rpm to obtain a mixture E;
step six: 1g of chitosan is added into 99mL of the mixture C, and the mixture C is mixed for 30min under the magnetic stirring with the rotating speed of 150rpm, so as to obtain a mixture F;
step seven: mixing the mixture A, the mixture B and the mixture E in a volume ratio of 1:1:1, and mixing for 5min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture G;
step eight: adding 10mL of the mixture G into 90mL of the mixture F, and mixing for 30min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture H;
step nine: 2mL of the mixture D was added to 98mL of the mixture H at a rate of 2mL/min, and the mixture was mixed for 30min under magnetic stirring at a rotation speed of 150rpm, to obtain a pesticidal and antifungal tobacco mellowing composition.
Examples 1 to 4: insecticidal mildew-proof tobacco mellowing composition and preparation method thereof
The embodiment provides a tobacco mellowing composition for killing insects and preventing mildew, wherein the components of the tobacco mellowing composition comprise glucose oxidase, catalase, glucose, mildew preventive (dimethyl fumarate, sodium diacetate and calcium propionate), chitosan, glycerol and acetic acid.
The preparation method of the insecticidal mildew-proof tobacco mellowing composition comprises the following steps:
step one: adding 0.2g of glucose oxidase and 0.2g of catalase into 100mL of distilled water, mixing for 2min under magnetic stirring at a rotation speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture A;
step two: adding 10g of glucose into 90mL of distilled water, mixing for 2min under magnetic stirring at a rotation speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture B;
step three: adding 1mL of acetic acid into 99mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture C;
step four: adding 6mL of glycerol into 94mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture D;
step five: 0.05g of dimethyl fumarate, 0.05g of sodium diacetate and 0.05g of calcium propionate were added to 100mL of distilled water, and mixed for 2min under magnetic stirring at 150rpm to obtain a mixture E;
step six: 1g of chitosan is added into 99mL of the mixture C, and the mixture C is mixed for 30min under the magnetic stirring with the rotating speed of 150rpm, so as to obtain a mixture F;
step seven: mixing the mixture A, the mixture B and the mixture E in a volume ratio of 1:1:1, and mixing for 5min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture G;
step eight: adding 10mL of the mixture G into 90mL of the mixture F, and mixing for 30min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture H;
step nine: 2mL of the mixture D was added to 98mL of the mixture H at a rate of 2mL/min, and the mixture was mixed for 30min under magnetic stirring at a rotation speed of 150rpm, to obtain a pesticidal and antifungal tobacco mellowing composition.
Examples 1 to 5: insecticidal mildew-proof tobacco mellowing composition and preparation method thereof
The embodiment provides a tobacco mellowing composition for killing insects and preventing mildew, wherein the components of the tobacco mellowing composition comprise glucose oxidase, catalase, glucose, a mildew preventive (sodium diacetate), chitosan, glycerol and acetic acid.
The preparation method of the insecticidal mildew-proof tobacco mellowing composition comprises the following steps:
step one: adding 0.05g of glucose oxidase and 0.05g of catalase into 100mL of distilled water, mixing for 2min under magnetic stirring at a rotation speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture A;
step two: adding 1g of glucose into 99mL of distilled water, mixing for 2min under magnetic stirring at a rotating speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture B;
step three: adding 1mL of acetic acid into 99mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture C;
step four: adding 6mL of glycerol into 94mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture D;
step five: adding 0.2g of sodium diacetate into 100mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture E;
step six: 1g of chitosan is added into 99mL of the mixture C, and the mixture C is mixed for 30min under the magnetic stirring with the rotating speed of 150rpm, so as to obtain a mixture F;
step seven: mixing the mixture A, the mixture B and the mixture E in a volume ratio of 1:1:1, and mixing for 5min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture G;
step eight: adding 10mL of the mixture G into 90mL of the mixture F, and mixing for 30min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture H;
step nine: 2mL of the mixture D was added to 98mL of the mixture H at a rate of 2mL/min, and the mixture was mixed for 30min under magnetic stirring at a rotation speed of 150rpm, to obtain a pesticidal and antifungal tobacco mellowing composition.
Examples 1 to 6: insecticidal mildew-proof tobacco mellowing composition and preparation method thereof
The embodiment provides a tobacco mellowing composition for killing insects and preventing mildew, wherein the components of the tobacco mellowing composition comprise glucose oxidase, catalase, glucose, a mildew preventive (sodium diacetate), chitosan, glycerol and acetic acid.
The preparation method of the insecticidal mildew-proof tobacco mellowing composition comprises the following steps:
step one: adding 0.1g of glucose oxidase and 0.1g of catalase into 100mL of distilled water, mixing for 2min under magnetic stirring at a rotation speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture A;
step two: adding 3g of glucose into 97mL of distilled water, mixing for 2min under magnetic stirring at a rotating speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture B;
step three: adding 1mL of acetic acid into 99mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture C;
step four: adding 6mL of glycerol into 94mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture D;
step five: adding 0.2g of sodium diacetate into 100mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture E;
step six: 1g of chitosan is added into 99mL of the mixture C, and the mixture C is mixed for 30min under the magnetic stirring with the rotating speed of 150rpm, so as to obtain a mixture F;
step seven: mixing the mixture A, the mixture B and the mixture E in a volume ratio of 1:1:1, and mixing for 5min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture G;
step eight: adding 10mL of the mixture G into 90mL of the mixture F, and mixing for 30min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture H;
step nine: 2mL of the mixture D was added to 98mL of the mixture H at a rate of 2mL/min, and the mixture was mixed for 30min under magnetic stirring at a rotation speed of 150rpm, to obtain a pesticidal and antifungal tobacco mellowing composition.
Examples 1 to 7: insecticidal mildew-proof tobacco mellowing composition and preparation method thereof
The embodiment provides a tobacco mellowing composition for killing insects and preventing mildew, wherein the components of the tobacco mellowing composition comprise glucose oxidase, catalase, glucose, a mildew preventive (sodium diacetate), chitosan, glycerol and acetic acid.
The preparation method of the insecticidal mildew-proof tobacco mellowing composition comprises the following steps:
step one: adding 0.15g of glucose oxidase and 0.15g of catalase into 100mL of distilled water, mixing for 2min under magnetic stirring at a rotation speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture A;
step two: adding 6g of glucose into 94mL of distilled water, mixing for 2min under magnetic stirring at a rotation speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture B;
step three: adding 1mL of acetic acid into 99mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture C;
step four: adding 6mL of glycerol into 94mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture D;
step five: adding 0.2g of sodium diacetate into 100mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture E;
step six: 1g of chitosan is added into 99mL of the mixture C, and the mixture C is mixed for 30min under the magnetic stirring with the rotating speed of 150rpm, so as to obtain a mixture F;
step seven: mixing the mixture A, the mixture B and the mixture E in a volume ratio of 1:1:1, and mixing for 5min under magnetic stirring at 150rpm to obtain a mixture G;
step eight: adding 10mL of the mixture G into 90mL of the mixture F, and mixing for 30min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture H;
step nine: 2mL of the mixture D was added to 98mL of the mixture H at a rate of 2mL/min, and the mixture was mixed for 30min under magnetic stirring at a rotation speed of 150rpm, to obtain a pesticidal and antifungal tobacco mellowing composition.
Examples 1 to 8: insecticidal mildew-proof tobacco mellowing composition and preparation method thereof
The embodiment provides a tobacco mellowing composition for killing insects and preventing mildew, wherein the components of the tobacco mellowing composition comprise glucose oxidase, catalase, glucose, a mildew preventive (sodium diacetate), chitosan, glycerol and acetic acid.
The preparation method of the insecticidal mildew-proof tobacco mellowing composition comprises the following steps:
step one: adding 0.2g of glucose oxidase and 0.2g of catalase into 100mL of distilled water, mixing for 2min under magnetic stirring at a rotation speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture A;
step two: adding 10g of glucose into 90mL of distilled water, mixing for 2min under magnetic stirring at a rotation speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture B;
step three: adding 1mL of acetic acid into 99mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture C;
step four: adding 6mL of glycerol into 94mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture D;
step five: adding 0.2g of sodium diacetate into 100mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture E;
step six: 1g of chitosan is added into 99mL of the mixture C, and the mixture C is mixed for 30min under the magnetic stirring with the rotating speed of 150rpm, so as to obtain a mixture F;
step seven: mixing the mixture A, the mixture B and the mixture E in a volume ratio of 1:1:1, and mixing for 5min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture G;
step eight: adding 10mL of the mixture G into 90mL of the mixture F, and mixing for 30min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture H;
step nine: 2mL of the mixture D was added to 98mL of the mixture H at a rate of 2mL/min, and the mixture was mixed for 30min under magnetic stirring at a rotation speed of 150rpm, to obtain a pesticidal and antifungal tobacco mellowing composition.
Comparative examples 1-1: tobacco mellowing composition and preparation method thereof
The present comparative example provides a tobacco mellowing composition whose ingredients include a mildewproof agent (dimethyl fumarate, sodium diacetate, and calcium propionate), chitosan, glycerin, and acetic acid.
The preparation method of the tobacco mellowing composition comprises the following steps:
step one: adding 1mL of acetic acid into 99mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture C;
step two: adding 6mL of glycerol into 94mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture D;
step three: 0.05g of dimethyl fumarate, 0.05g of sodium diacetate and 0.05g of calcium propionate were added to 100mL of distilled water, and mixed for 2min under magnetic stirring at 150rpm to obtain a mixture E;
step four: 1g of chitosan is added into 99mL of the mixture C, and the mixture C is mixed for 30min under the magnetic stirring with the rotating speed of 150rpm, so as to obtain a mixture F;
step five: 7mL of the mixture E is added into 93mL of the mixture F, and the mixture F is mixed for 30min under magnetic stirring at a rotating speed of 150rpm, so as to obtain a mixture H;
step six: 2mL of the mixture D was added to 98mL of the mixture H at a rate of 2mL/min, and the mixture was mixed for 30min under magnetic stirring at a rotation speed of 150rpm, to obtain a tobacco mellowing composition.
Comparative examples 1-2: tobacco mellowing composition and preparation method thereof
The present comparative example provides a tobacco mellowing composition comprising components of glucose oxidase, catalase, glucose, chitosan, glycerol, and acetic acid.
The preparation method of the tobacco mellowing composition comprises the following steps:
step one: adding 0.2g of glucose oxidase and 0.2g of catalase into 100mL of distilled water, mixing for 2min under magnetic stirring at a rotation speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture A;
step two: adding 10g of glucose into 90mL of distilled water, mixing for 2min under magnetic stirring at a rotation speed of 150rpm, and performing sterile filtration by using a filter membrane with a pore diameter of 0.22 mu m to obtain a mixture B;
step three: adding 1mL of acetic acid into 99mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture C;
step four: adding 6mL of glycerol into 94mL of distilled water, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture D;
step five: 1g of chitosan is added into 99mL of the mixture C, and the mixture C is mixed for 30min under the magnetic stirring with the rotating speed of 150rpm, so as to obtain a mixture F;
step six: mixing the mixture A and the mixture B in a volume ratio of 1:1, and mixing for 2min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture G;
step seven: adding 8mL of the mixture G into 92mL of the mixture F, and mixing for 30min under magnetic stirring at a rotating speed of 150rpm to obtain a mixture H;
step eight: 2mL of the mixture D was added to 98mL of the mixture H at a rate of 2mL/min, and the mixture was mixed for 30min under magnetic stirring at a rotation speed of 150rpm, to obtain a tobacco mellowing composition.
Experimental example 1: insect-killing and mildew-proof performance experiment of tobacco mellowing composition
The experimental example provides an insecticidal and mildew-proof performance experiment of a tobacco mellowing composition, and the experimental process is as follows:
taking 5kg of insect-grown tobacco leaves, uniformly spraying tobacco mellowing compositions of examples 1-1 to 1-8 and comparative examples 1-1 to 1-2 on tobacco leaves according to the proportion of 2% of the weight of the tobacco leaves, respectively sealing by using plastic films, and placing the tobacco leaves in a storage chamber, wherein the temperature of the storage chamber is 27+/-2 ℃ and the humidity of the storage chamber is 60+/-6%; after 60 days, detecting the insecticidal effect of the tobacco mellowing composition by observing whether pests are active or not and whether the pests are stiff or not through naked eyes, wherein the percentage of the number (head) of the inactive pests or the pests which are stiff to the total number (head) of the pests is the pest mortality; detecting the mildew-proof effect of the tobacco mellowing composition by observing whether the tobacco leaves are mildewed or not through naked eyes, wherein the percentage of the weight of the mildewed whole tobacco leaves to the weight of the total tobacco leaves is the mildew rate; the experimental results are shown in Table 1.
As can be seen from Table 1, when the tobacco mellowing compositions of examples 1-1 to 1-8 were used to mellowing tobacco leaves, the mortality of pests in the tobacco leaves reached 100%, and no mildew occurred; when the tobacco mellowing composition of comparative example 1-1 is used for mellowing tobacco leaves, the composition does not contain glucose oxidase and catalase, and in natural mellowing of tobacco leaves, the oxygen concentration cannot be kept at a low level, and pests cannot be effectively killed; when the tobacco mellowing composition of comparative examples 1-2 was used to mellowing tobacco, 10% of the tobacco was mildewed during natural mellowing of the tobacco, since the composition did not contain the mildewproof agent.
Table 1 results of insecticidal and fungicidal Properties of tobacco mellowing compositions
| Pest mortality/% | Mildew rate/% | |
| Example 1-1 | 100 | Without any means for |
| Examples 1 to 2 | 100 | Without any means for |
| Examples 1 to 3 | 100 | Without any means for |
| Examples 1 to 4 | 100 | Without any means for |
| Examples 1 to 5 | 100 | Without any means for |
| Examples 1 to 6 | 100 | Without any means for |
| Examples 1 to 7 | 100 | Without any means for |
| Examples 1 to 8 | 100 | Without any means for |
| Comparative examples 1 to 1 | 42 | Without any means for |
| Comparative examples 1 to 2 | 90 | 10 |
It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the invention.
Claims (10)
1. The insecticidal mildew-proof tobacco mellowing composition is characterized by comprising glucose oxidase, catalase, glucose, mildew inhibitor, chitosan, glycerol, acetic acid and water.
2. The tobacco mellowing composition according to claim 1, wherein the components of the tobacco mellowing composition comprise, by weight, 0.0016 to 0.0065 parts of glucose oxidase, 0.0016 to 0.0065 parts of catalase, 0.033 to 0.33 parts of glucose, 0.0016 to 0.0065 parts of mildew inhibitor, 0.882 parts of chitosan, 0.156 parts of glycerol, 0.0873 parts of acetic acid, and 98.5252 to 98.8369 parts of water.
3. The tobacco mellowing composition according to claim 1 or 2, wherein the mildew preventive comprises one or more of dimethyl fumarate, sodium diacetate and calcium propionate.
4. A tobacco leaf alcoholizing composition according to any one of claims 1 to 3 wherein the glucose oxidase is a food grade glucose oxidase with an enzyme activity of greater than or equal to 10000U/g.
5. A tobacco mellowing composition according to any one of claims 1 to 3, wherein the catalase is a food grade catalase with an enzyme activity of not less than 100000U/g.
6. A method for preparing the insecticidal and mildewproof tobacco mellowing composition according to any one of claims 1 to 5, which comprises adding an aqueous glycerol solution to a mixed aqueous solution of glucose oxidase, catalase, glucose, acetic acid, a mildewproof agent and chitosan, and mixing to obtain the insecticidal and mildewproof tobacco mellowing composition.
7. The method according to claim 6, characterized in that the method comprises the steps of:
step one: adding glucose oxidase and catalase into water, and mixing to obtain a mixture A;
step two: adding glucose into water, and mixing to obtain a mixture B;
step three: adding acetic acid into water, and mixing to obtain a mixture C;
step four: adding glycerol into water, and mixing to obtain a mixture D;
step five: adding a mildew preventive into water, and mixing to obtain a mixture E;
step six: adding chitosan into the mixture C, and mixing to obtain a mixture F;
step seven: mixing the mixture A, the mixture B and the mixture E to obtain a mixture G;
step eight: adding the mixture G into the mixture F, and mixing to obtain a mixture H;
step nine: and adding the mixture D into the mixture H, and mixing to obtain the insecticidal and mildew-proof tobacco mellowing composition.
8. The method of claim 7, wherein the first step is: adding 0.05-0.2 g of glucose oxidase and 0.05-0.2 g of catalase into 100mL of water, and mixing to obtain a mixture A;
the second step is as follows: adding 1-10 g of glucose into 90-99 mL of water, and mixing to obtain a mixture B;
the third step is as follows: adding 1mL of acetic acid into 99mL of water, and mixing to obtain a mixture C;
the fourth step is as follows: adding 6mL of glycerol into 94mL of water, and mixing to obtain a mixture D;
the fifth step is as follows: adding 0.05-0.2 g of mildew inhibitor into 100mL of water, and mixing to obtain a mixture E;
the sixth step is as follows: adding 1g of chitosan into 99mL of the mixture C, and mixing to obtain a mixture F;
the seventh step is as follows: mixing the mixture A, the mixture B and the mixture E in a volume ratio of 1:1:1 to obtain a mixture G;
the step eight is as follows: adding 10mL of the mixture G into 90mL of the mixture F, and mixing to obtain a mixture H;
in the step nine, 2mL of the mixture D is added into 98mL of the mixture H at a rate of 1-2 mL/min, and the mixture is mixed to obtain the insecticidal mildew-proof tobacco mellowing composition.
9. The method of claim 8, wherein the rate in step nine is 2mL/min.
10. Use of the insecticidal and mildewproof tobacco mellowing composition according to any one of claims 1 to 5 or the method for preparing the insecticidal and mildewproof tobacco mellowing composition according to any one of claims 6 to 9 for tobacco mellowing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310217451.XA CN116076502A (en) | 2023-03-08 | 2023-03-08 | Insecticidal mildew-proof tobacco mellowing composition, and preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310217451.XA CN116076502A (en) | 2023-03-08 | 2023-03-08 | Insecticidal mildew-proof tobacco mellowing composition, and preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116076502A true CN116076502A (en) | 2023-05-09 |
Family
ID=86208428
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310217451.XA Pending CN116076502A (en) | 2023-03-08 | 2023-03-08 | Insecticidal mildew-proof tobacco mellowing composition, and preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116076502A (en) |
-
2023
- 2023-03-08 CN CN202310217451.XA patent/CN116076502A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8017667B2 (en) | Antimicrobial packaging material | |
| KR100319252B1 (en) | Antibacterial and Antifungal Composition for Food and Food related Instruments | |
| CN107156289A (en) | A kind of fresh-keeping of vegetables bactericidal agent and preparation method thereof | |
| CN108967535A (en) | A kind of fresh-keeping microcapsules, preparation method and application | |
| CN116076502A (en) | Insecticidal mildew-proof tobacco mellowing composition, and preparation method and application thereof | |
| CN100353871C (en) | Granular concave rod alcohol antistaling agent | |
| CN103739394A (en) | Biogas liquid compounded fungicide for preventing and treating gray mold | |
| CN1915041A (en) | Product of antistaling agent for vegetable, fruit, and its applications | |
| US20030100254A1 (en) | Method of sterilizing mammal carcasses | |
| JPH07500243A (en) | Natamycin treatment of dried whole grains | |
| CN112191085B (en) | Preparation method of garbage deodorant | |
| KR100317482B1 (en) | Nutrient agent of kim-chi containing water-soluble chitosan and using method thereof | |
| KR19980028332A (en) | Vegetable antibacterial agent | |
| CN1158939C (en) | Mulberry preserving agent | |
| KR20040092144A (en) | Antibacterial and Antifungal Composition Including Fennel Extract | |
| CN102067902A (en) | Meat product antistaling agent | |
| AU2005256153B2 (en) | Antimicrobial packaging material | |
| KR20040083742A (en) | Antimicrobial composition comprising grape fruit seed extract and sodium bicarbonate and preparation method thereof | |
| CN103755471A (en) | Biogas slurry compound bactericide for preventing and treating leaf mold | |
| KR20140016114A (en) | Environment-friendly deodorant using microorganism fermentation and charcoal | |
| KR100288963B1 (en) | fresh degree conservation materials | |
| KR102307746B1 (en) | Wet tissue composition and wet tissue comprising the same | |
| CN102771501A (en) | Composite mildewproof and antibacterial agent and its preparation method | |
| KR100308414B1 (en) | fresh degree conservation materials | |
| CN103772014A (en) | Composite biogas slurry bactericide for preventing and controlling soft rot |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |