CN116057180A - 用于表观基因组编辑的组合物和方法 - Google Patents

用于表观基因组编辑的组合物和方法 Download PDF

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Publication number
CN116057180A
CN116057180A CN202180047868.5A CN202180047868A CN116057180A CN 116057180 A CN116057180 A CN 116057180A CN 202180047868 A CN202180047868 A CN 202180047868A CN 116057180 A CN116057180 A CN 116057180A
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China
Prior art keywords
seq
fusion protein
amino acid
sequence
domain
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Pending
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CN202180047868.5A
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English (en)
Chinese (zh)
Inventor
L·吉尔伯特
J·韦斯曼
J·努涅斯
G·波米叶
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University of California San Diego UCSD
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University of California San Diego UCSD
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Publication of CN116057180A publication Critical patent/CN116057180A/zh
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/73—Fusion polypeptide containing domain for protein-protein interaction containing coiled-coiled motif (leucine zippers)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/85—Fusion polypeptide containing an RNA binding domain
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Steroid Compounds (AREA)
  • Macromonomer-Based Addition Polymer (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Detergent Compositions (AREA)
CN202180047868.5A 2020-06-05 2021-06-04 用于表观基因组编辑的组合物和方法 Pending CN116057180A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202063035431P 2020-06-05 2020-06-05
US63/035,431 2020-06-05
US202063118832P 2020-11-27 2020-11-27
US63/118,832 2020-11-27
PCT/US2021/035937 WO2021248023A2 (en) 2020-06-05 2021-06-04 Compositions and methods for epigenome editing

Publications (1)

Publication Number Publication Date
CN116057180A true CN116057180A (zh) 2023-05-02

Family

ID=78831718

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180047868.5A Pending CN116057180A (zh) 2020-06-05 2021-06-04 用于表观基因组编辑的组合物和方法

Country Status (12)

Country Link
US (1) US20230212323A1 (https=)
EP (1) EP4162054A4 (https=)
JP (1) JP2023529844A (https=)
KR (1) KR20230021081A (https=)
CN (1) CN116057180A (https=)
AU (1) AU2021282659A1 (https=)
BR (1) BR112022024747A2 (https=)
CA (1) CA3184882A1 (https=)
GB (1) GB2612466B (https=)
IL (1) IL298605A (https=)
MX (1) MX2022015284A (https=)
WO (1) WO2021248023A2 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113846019B (zh) * 2021-03-05 2023-08-01 海南师范大学 一种海洋微拟球藻靶向表观基因组遗传调控方法
KR20250010025A (ko) * 2022-05-13 2025-01-20 인테그라 테라퓨틱스 도입유전자 발현 및 핵 국소화를 개선하기 위한 트랜스포사제의 용도
KR20250165443A (ko) 2023-02-17 2025-11-25 화이트헤드 인스티튜트 포 바이오메디칼 리서치 후성유전학적 변형 제조를 위한 조성물 및 방법
WO2024238682A2 (en) * 2023-05-15 2024-11-21 The Regents Of The University Of California Cask gene editing system

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102348715A (zh) * 2009-02-03 2012-02-08 阿穆尼克斯运营公司 延伸重组多肽和包含该延伸重组多肽的组合物
WO2014152432A2 (en) * 2013-03-15 2014-09-25 The General Hospital Corporation Rna-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci
WO2018140362A1 (en) * 2017-01-26 2018-08-02 The Regents Of The University Of California Targeted gene demethylation in plants
CN109982710A (zh) * 2016-09-13 2019-07-05 杰克逊实验室 靶向增强的dna去甲基化

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9115386B2 (en) * 2008-09-26 2015-08-25 Children's Medical Center Corporation Selective oxidation of 5-methylcytosine by TET-family proteins
US10077453B2 (en) * 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
US20190100732A1 (en) * 2016-06-02 2019-04-04 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Assay for the removal of methyl-cytosine residues from dna
KR102922694B1 (ko) * 2018-04-19 2026-02-03 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 유전자 편집을 위한 조성물 및 방법

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102348715A (zh) * 2009-02-03 2012-02-08 阿穆尼克斯运营公司 延伸重组多肽和包含该延伸重组多肽的组合物
WO2014152432A2 (en) * 2013-03-15 2014-09-25 The General Hospital Corporation Rna-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci
CN109982710A (zh) * 2016-09-13 2019-07-05 杰克逊实验室 靶向增强的dna去甲基化
WO2018140362A1 (en) * 2017-01-26 2018-08-02 The Regents Of The University Of California Targeted gene demethylation in plants

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Publication number Publication date
CA3184882A1 (en) 2021-12-09
AU2021282659A1 (en) 2023-01-05
GB202219608D0 (en) 2023-02-08
IL298605A (en) 2023-01-01
MX2022015284A (es) 2023-01-19
BR112022024747A2 (pt) 2023-03-07
EP4162054A2 (en) 2023-04-12
WO2021248023A2 (en) 2021-12-09
EP4162054A4 (en) 2025-01-22
GB2612466A (en) 2023-05-03
WO2021248023A3 (en) 2022-01-27
JP2023529844A (ja) 2023-07-12
KR20230021081A (ko) 2023-02-13
GB2612466B (en) 2026-04-01
US20230212323A1 (en) 2023-07-06

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