GB2612466B - Compositions and methods for epigenome editing - Google Patents

Compositions and methods for epigenome editing

Info

Publication number
GB2612466B
GB2612466B GB2219608.3A GB202219608A GB2612466B GB 2612466 B GB2612466 B GB 2612466B GB 202219608 A GB202219608 A GB 202219608A GB 2612466 B GB2612466 B GB 2612466B
Authority
GB
United Kingdom
Prior art keywords
compositions
methods
epigenome editing
epigenome
editing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
GB2219608.3A
Other languages
English (en)
Other versions
GB202219608D0 (en
GB2612466A (en
Inventor
Gilbert Luke
Weissman Jonathan
Nunez James
Pommier Greg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California Berkeley
University of California San Diego UCSD
Original Assignee
University of California Berkeley
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California Berkeley, University of California San Diego UCSD filed Critical University of California Berkeley
Publication of GB202219608D0 publication Critical patent/GB202219608D0/en
Publication of GB2612466A publication Critical patent/GB2612466A/en
Application granted granted Critical
Publication of GB2612466B publication Critical patent/GB2612466B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K19/00Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0071Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y114/00Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/11Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors (1.14.11)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/70Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/73Fusion polypeptide containing domain for protein-protein interaction containing coiled-coiled motif (leucine zippers)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/80Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/85Fusion polypeptide containing an RNA binding domain
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00Structure or type of the nucleic acid
    • C12N2310/50Physical structure
    • C12N2310/53Physical structure partially self-complementary or closed
    • C12N2310/531Stem-loop; Hairpin

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Steroid Compounds (AREA)
  • Macromonomer-Based Addition Polymer (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Detergent Compositions (AREA)
GB2219608.3A 2020-06-05 2021-06-04 Compositions and methods for epigenome editing Active GB2612466B (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063035431P 2020-06-05 2020-06-05
US202063118832P 2020-11-27 2020-11-27
PCT/US2021/035937 WO2021248023A2 (en) 2020-06-05 2021-06-04 Compositions and methods for epigenome editing

Publications (3)

Publication Number Publication Date
GB202219608D0 GB202219608D0 (en) 2023-02-08
GB2612466A GB2612466A (en) 2023-05-03
GB2612466B true GB2612466B (en) 2026-04-01

Family

ID=78831718

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2219608.3A Active GB2612466B (en) 2020-06-05 2021-06-04 Compositions and methods for epigenome editing

Country Status (12)

Country Link
US (1) US20230212323A1 (https=)
EP (1) EP4162054A4 (https=)
JP (1) JP2023529844A (https=)
KR (1) KR20230021081A (https=)
CN (1) CN116057180A (https=)
AU (1) AU2021282659A1 (https=)
BR (1) BR112022024747A2 (https=)
CA (1) CA3184882A1 (https=)
GB (1) GB2612466B (https=)
IL (1) IL298605A (https=)
MX (1) MX2022015284A (https=)
WO (1) WO2021248023A2 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113846019B (zh) * 2021-03-05 2023-08-01 海南师范大学 一种海洋微拟球藻靶向表观基因组遗传调控方法
KR20250010025A (ko) * 2022-05-13 2025-01-20 인테그라 테라퓨틱스 도입유전자 발현 및 핵 국소화를 개선하기 위한 트랜스포사제의 용도
KR20250165443A (ko) 2023-02-17 2025-11-25 화이트헤드 인스티튜트 포 바이오메디칼 리서치 후성유전학적 변형 제조를 위한 조성물 및 방법
WO2024238682A2 (en) * 2023-05-15 2024-11-21 The Regents Of The University Of California Cask gene editing system

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9115386B2 (en) * 2008-09-26 2015-08-25 Children's Medical Center Corporation Selective oxidation of 5-methylcytosine by TET-family proteins
ES2610356T3 (es) * 2009-02-03 2017-04-27 Amunix Operating Inc. Polipéptidos recombinantes extendidos y composiciones que comprenden los mismos
CN112301024A (zh) * 2013-03-15 2021-02-02 通用医疗公司 使用RNA引导的FokI核酸酶(RFN)提高RNA引导的基因组编辑的特异性
US10077453B2 (en) * 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
US20190100732A1 (en) * 2016-06-02 2019-04-04 Yissum Research Development Company Of The Hebrew University Of Jerusalem Ltd. Assay for the removal of methyl-cytosine residues from dna
WO2018053035A1 (en) * 2016-09-13 2018-03-22 The Jackson Laboratory Targeted dna demethylation and methylation
AU2018213044B2 (en) * 2017-01-26 2024-08-01 The Regents Of The University Of California Targeted gene demethylation in plants
KR102922694B1 (ko) * 2018-04-19 2026-02-03 더 리젠츠 오브 더 유니버시티 오브 캘리포니아 유전자 편집을 위한 조성물 및 방법

Also Published As

Publication number Publication date
CA3184882A1 (en) 2021-12-09
AU2021282659A1 (en) 2023-01-05
GB202219608D0 (en) 2023-02-08
IL298605A (en) 2023-01-01
MX2022015284A (es) 2023-01-19
BR112022024747A2 (pt) 2023-03-07
EP4162054A2 (en) 2023-04-12
WO2021248023A2 (en) 2021-12-09
EP4162054A4 (en) 2025-01-22
CN116057180A (zh) 2023-05-02
GB2612466A (en) 2023-05-03
WO2021248023A3 (en) 2022-01-27
JP2023529844A (ja) 2023-07-12
KR20230021081A (ko) 2023-02-13
US20230212323A1 (en) 2023-07-06

Similar Documents

Publication Publication Date Title
GB202311318D0 (en) Compositions and methods for epigenetic editing
GB2612466B (en) Compositions and methods for epigenome editing
GB202011871D0 (en) Composition and method
GB202111040D0 (en) Compositions and methods
GB202108585D0 (en) Methods and compositions
IL317701A (en) Epigenetic editing compositions and methods
IL300332B2 (en) Composition and method
GB2593010B (en) Composition and Method
GB202100997D0 (en) Composition and method
GB202100998D0 (en) Composition and method
GB202010449D0 (en) Method and composition
HK40093323A (en) Compositions and methods for epigenome editing
GB202109827D0 (en) Method and composition
GB202102310D0 (en) Composition and method
GB202110091D0 (en) Methods and compositions
GB202016644D0 (en) Composition and method
GB2603642B (en) Method and composition
GB202211117D0 (en) Compositions and methods for non-immunogenecity
GB202113085D0 (en) Composition and method
GB202113086D0 (en) Composition and method
GB202108601D0 (en) Composition and method
GB202105673D0 (en) Method and composition
GB202018825D0 (en) Composition and method
GB202016617D0 (en) Composition and method
GB202016629D0 (en) Composition and method