CN116004544A - 基于使用表达增强性基因座来制备抗体的组合物和方法 - Google Patents

基于使用表达增强性基因座来制备抗体的组合物和方法 Download PDF

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CN116004544A
CN116004544A CN202211344339.4A CN202211344339A CN116004544A CN 116004544 A CN116004544 A CN 116004544A CN 202211344339 A CN202211344339 A CN 202211344339A CN 116004544 A CN116004544 A CN 116004544A
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nucleic acid
nucleotide sequence
rrs
cell
sequence encoding
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R·巴布
D·布拉科夫
陈刚
J·P·梵迪
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46Hybrid immunoglobulins
    • C07K16/468Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6897Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids involving reporter genes operably linked to promoters
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/30Immunoglobulins specific features characterized by aspects of specificity or valency
    • C07K2317/31Immunoglobulins specific features characterized by aspects of specificity or valency multispecific
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/51Complete heavy chain or Fd fragment, i.e. VH + CH1
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515Complete light chain, i.e. VL + CL
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52Constant or Fc region; Isotype
    • C07K2317/526CH3 domain
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565Complementarity determining region [CDR]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production

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  • Organic Chemistry (AREA)
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  • Peptides Or Proteins (AREA)
CN202211344339.4A 2016-04-20 2017-04-20 基于使用表达增强性基因座来制备抗体的组合物和方法 Pending CN116004544A (zh)

Applications Claiming Priority (4)

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US201662325385P 2016-04-20 2016-04-20
US62/325,385 2016-04-20
PCT/US2017/028552 WO2017184831A1 (en) 2016-04-20 2017-04-20 Compositions and methods for making antibodies based on use of an expression-enhancing locus
CN201780024560.2A CN109071633B (zh) 2016-04-20 2017-04-20 基于使用表达增强性基因座来制备抗体的组合物和方法

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EP (1) EP3445780A1 (enExample)
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KR (2) KR102474757B1 (enExample)
CN (2) CN109071633B (enExample)
AR (1) AR108295A1 (enExample)
AU (2) AU2017253240B2 (enExample)
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JP7208380B2 (ja) * 2018-10-26 2023-01-18 エフ.ホフマン-ラ ロシュ アーゲー リコンビナーゼ媒介性カセット交換を使用した多重特異性抗体スクリーニング法
CN113631711B (zh) * 2018-12-21 2025-03-04 豪夫迈·罗氏有限公司 核酸的靶向整合
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BR112021025401A2 (pt) 2019-06-19 2022-02-01 Hoffmann La Roche Métodos para produzir um anticorpo trivalente, ácido desoxirribonucleico, uso de um ácido desoxirribonucleico, célula de mamífero recombinante, composição e método para produzir uma célula de mamífero recombinante
WO2020254355A1 (en) 2019-06-19 2020-12-24 F. Hoffmann-La Roche Ag Method for the generation of a bivalent, bispecific antibody expressing cell by targeted integration of multiple expression cassettes in a defined organization
BR112021025436A2 (pt) * 2019-06-19 2022-02-01 Hoffmann La Roche Métodos para produzir um anticorpo biespecífico trivalente, biespecífico bivalente, biespecífico multivalente, para produzir uma célula de mamífero recombinante e para produzir um anticorpo trivalente, ácidos desoxirribonucleicos, usos de um ácido desoxirribonucleico, células de mamífero recombinante, composições e uso de mrna de recombinase cre
WO2020254357A1 (en) 2019-06-19 2020-12-24 F. Hoffmann-La Roche Ag Method for the generation of a protein expressing cell by targeted integration using cre mrna
CA3140323A1 (en) * 2019-06-19 2020-12-24 Johannes Auer Method for the generation of a multivalent, bispecific antibody expressing cell by targeted integration of multiple expression cassettes in a defined organization
KR20220025806A (ko) * 2019-06-26 2022-03-03 제넨테크, 인크. 핵산의 무작위 구성 표적화 통합
EP4087925A2 (en) * 2020-01-10 2022-11-16 F. Hoffmann-La Roche AG Method for the assembly of large nucleic acids from short fragments
TW202342755A (zh) * 2021-12-22 2023-11-01 美商建南德克公司 多載體重組酶介導的匣式交換
TW202528546A (zh) 2023-09-07 2025-07-16 美商再生元醫藥公司 經共價表面修飾之腺相關病毒的生產及純化
CN119955730A (zh) * 2023-11-08 2025-05-09 深圳太力生物技术有限责任公司 一种细胞株及其制备方法与应用
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IL262268B2 (en) 2024-05-01
KR102474757B1 (ko) 2022-12-07
AU2024220126A1 (en) 2024-10-17
EA201892137A1 (ru) 2019-03-29
CN109071633A (zh) 2018-12-21
CN109071633B (zh) 2022-11-18
JP2022164824A (ja) 2022-10-27
JP7134868B2 (ja) 2022-09-12
KR20210135340A (ko) 2021-11-12
AU2017253240B2 (en) 2024-07-04
EP3445780A1 (en) 2019-02-27
SG10202010156XA (en) 2020-11-27
WO2017184831A1 (en) 2017-10-26
KR20180134894A (ko) 2018-12-19
JP7789143B2 (ja) 2025-12-19
MX2024014381A (es) 2024-12-06
JP2024164196A (ja) 2024-11-26
IL262268B1 (en) 2024-01-01
JP2019514358A (ja) 2019-06-06
CA3015371A1 (en) 2017-10-26
US11530277B2 (en) 2022-12-20
MX2018012866A (es) 2019-03-11
SG11201807881VA (en) 2018-10-30
TW201803981A (zh) 2018-02-01
IL262268A (en) 2018-11-29
US20190263937A1 (en) 2019-08-29
AU2017253240A1 (en) 2018-09-20
AR108295A1 (es) 2018-08-08
BR112018071285A2 (pt) 2019-02-12
JP7546015B2 (ja) 2024-09-05
IL309065A (en) 2024-02-01
US20230322956A1 (en) 2023-10-12

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