CN115997789A - Orchid disease control medicament and application method thereof - Google Patents
Orchid disease control medicament and application method thereof Download PDFInfo
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- CN115997789A CN115997789A CN202211647537.8A CN202211647537A CN115997789A CN 115997789 A CN115997789 A CN 115997789A CN 202211647537 A CN202211647537 A CN 202211647537A CN 115997789 A CN115997789 A CN 115997789A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
The invention discloses an orchid disease control medicament and a use method thereof, wherein the orchid disease control medicament comprises nano silver sol and polysaccharide solution; wherein the concentration of the nano silver sol is 5mg/L-1000mg/L calculated by silver content, and the concentration of the polysaccharide is 10mg/L-10000mg/L. The medicament can simultaneously realize the control of various diseases, and overcomes the defect of narrow antibacterial spectrum of the traditional pesticide; can realize the long-term antibacterial effect of once smearing, and solves the problems that the traditional pesticide volatilizes fast, can not act for a long time and needs repeated and timed spraying.
Description
Technical Field
The invention belongs to the field of nano materials, and particularly relates to application of nano silver sol in orchid disease control.
Background
Orchid is a worldwide rare flower and has extremely high ornamental value. However, orchid is susceptible to infection by pathogenic microorganisms such as bacteria, fungi and viruses during the breeding process, and various diseases such as anthracnose, southern blight, leaf blight, root rot, brown spot, virus diseases and the like appear. The diseases greatly affect the ornamental value of orchid and even lead to the death of orchid in a large area. Therefore, how to prevent and treat orchid diseases has become a key problem to be solved by orchid planting industry.
For the diseases mentioned above, spraying is conventionally carried out with the aid of the relevant pesticides. The pesticide spraying method has the following defects: (1) The single pesticide has a limited antibacterial spectrum, and generally can only kill one type of pathogenic microorganism, but is ineffective for other types of pathogenic microorganisms; (2) When spraying the pesticide, the pesticide components volatilize quickly, so that the pesticide is difficult to act for a long time and needs to be sprayed repeatedly and regularly; (3) Spraying the pesticide easily affects human health and causes environmental pollution. Therefore, development of efficient, safe and broad-spectrum antibacterial orchid disease control drugs and a using method thereof has become a key technical problem to be solved urgently.
Disclosure of Invention
The main object of the present invention is to provide an orchid disease control agent.
The technical scheme adopted for solving the technical problems is as follows:
the orchid disease preventing and controlling agent includes nanometer silver sol and polysaccharide solution; wherein the concentration of the nano silver sol is 5mg/L-1000mg/L calculated by silver content, and the concentration of the polysaccharide is 10mg/L-10000mg/L.
In the present invention, it is preferable that the particle diameter of the nano silver in the nano silver sol is 1 to 100nm.
In the present invention, the polysaccharide includes one or more of natural polysaccharide, natural polysaccharide degradation product, and natural polysaccharide chemical modification product.
In the present invention, the natural polysaccharide comprises one or more of the following polysaccharides: konjak glucomannan, chitosan, carrageenan, xanthan gum, algin, acacia gum, guar gum, water-soluble starch, carboxymethyl cellulose, soluble soybean polysaccharide, pectin and galactose mannans.
In the present invention, the natural polysaccharide degradation products include: powdered natural polysaccharide degradation products and natural polysaccharide degradation product solutions dissolved in water and organic solvents.
In the present invention, the natural polysaccharide degradation products include at least one of the following: natural polysaccharide hydrolysis products, natural polysaccharide alkaline hydrolysis products, natural polysaccharide polysaccharase hydrolysis products, natural polysaccharide products degraded by hydrogen peroxide, natural polysaccharide products degraded by gamma-rays, natural polysaccharide products degraded by ultrasonic waves and natural polysaccharide products degraded by nano metal photocatalysis.
The invention also provides application of the orchid disease control agent in orchid disease control.
Preferably, the orchid disease comprises one or more of anthracnose, southern blight, leaf blight, root rot, brown spot and virus disease.
The invention also provides a using method of the orchid disease control agent, which is characterized in that (1) when disease points are on leaves, the agent is directly and uniformly smeared on the disease positions, and then the whole orchid is placed under sunlight or is placed under ultraviolet irradiation for a sufficient time.
Preferably, the irradiation time under sunlight is 15min-5d, or the irradiation time under ultraviolet light is 5min-3000min.
Preferably, the whole orchid is irradiated under the sun light for 15min-300min or under the ultraviolet light for 5min-300min;
preferably, the ultraviolet light has a light intensity of 1.65X10 15 ～1.98×10 18 photons/sec/cm 3 。
When the disease points are in the rootstock, the root is completely soaked in the medicament for 10min-1000min, and then the root is taken out and drained, so that the plant can be planted in the soil.
The orchid disease control agent of the invention can kill common bacterial, fungal and viral pathogenic microorganisms of orchid. It has better control effect on common orchid diseases such as anthracnose, southern blight, leaf blight, root rot, brown spot and Bayesian virus.
The invention has the following technical effects and advantages:
(1) Can simultaneously realize the control of various diseases and overcome the defect of narrow antibacterial spectrum of the traditional pesticide;
(2) The antibacterial effect can be realized after the pesticide is smeared for 3 months, and the problems that the traditional pesticide volatilizes quickly and cannot act for a long time and needs to be sprayed repeatedly and regularly are solved;
(3) The application method or the soaking method is adopted, so that accurate medicine application is realized, and environmental pollution and influence on human health are avoided.
Detailed Description
Example 1
In the present embodiment, 10mL of nano silver sol (calculated by mass of silver) with concentration of 1000mg/L and 10mL of konjak glucomannan solution with concentration of 1000mg/L are mixed, and thus the orchid disease control agent containing 500mg/L nano silver sol (calculated by mass of silver) and 500mg/L konjak glucomannan is prepared.
Taking 0.5mL of the orchid disease control agent, smearing the agent on abnormal spot parts of leaves, placing the whole orchid in sunlight for 60min, and then placing the orchid in a shade place at normal temperature, wherein abnormal spots of the leaves disappear after 4 days.
Example 2
In the present embodiment, 10mL of nano silver sol (calculated by mass of silver) with the concentration of 500mg/L is mixed with 10mL of chitosan solution with the concentration of 500mg/L, and thus the orchid disease control medicament containing 250mg/L of nano silver sol (calculated by mass of silver) and 250mg/L of chitosan is prepared.
Taking 1mL of the orchid disease control agent, smearing the agent on abnormal spot positions of leaves, placing the whole orchid in sunlight for 90min, and then placing the orchid in a shade place at normal temperature, wherein the abnormal spots of the leaves disappear after 3 days.
Example 3
In the present embodiment, 10mL of nano silver sol (calculated by silver mass) with the concentration of 500mg/L is mixed with 10mL of acacia solution with the concentration of 500mg/L, and thus the orchid disease control agent containing 250mg/L nano silver sol (calculated by silver mass) and 250mg/L acacia is prepared.
1mL of the above orchid disease control agent was applied to abnormal spot portions of leaves, and the whole orchid was subjected to ultraviolet light (light intensity 1.65X10) 16 photons/sec/cm 3 ) After 120min of irradiation, the leaf is placed in a shade at normal temperature, and abnormal spots of the leaf disappear after 4 days.
Example 4
In the embodiment, 10mL of nano silver sol with the concentration of 800mg/L (calculated by silver mass) and 10mL of xanthan gum solution with the concentration of 1000mg/L are mixed, and thus the orchid disease control medicament containing 400mg/L of nano silver sol (calculated by silver mass) and 500mg/L of xanthan gum is prepared.
1mL of the above orchid disease control agent was applied to abnormal spot portions of leaves, and the whole orchid was subjected to ultraviolet light (light intensity of 2.0X10) 16 photons/sec/cm 3 ) After 60min of irradiation, the leaf is placed in a shade at normal temperature, and abnormal spots of the leaf disappear after 5 days.
Example 5
In the embodiment, 100mL of nano silver sol with the concentration of 600mg/L (calculated by silver mass) and 100mL of carrageenan solution with the concentration of 1000mg/L are mixed, and then the orchid disease control medicament containing 300mg/L of nano silver sol (calculated by silver mass) and 500mg/L carrageenan is prepared.
Soaking the root of orchid in the orchid disease control agent for 400min, taking out, draining, transplanting in normal culture medium, and eliminating root rot after 7 days.
Example 6
In the present embodiment, 100mL of nano silver sol (calculated by silver mass) with concentration of 1000mg/L and 100mL of algin solution with concentration of 300mg/L are mixed, and thus the orchid disease control agent containing 500mg/L nano silver sol (calculated by silver mass) and 150mg/L algin is prepared.
Soaking the roots of orchid in the orchid disease control agent for 200min, taking out, draining, transplanting in a normal culture medium, and eliminating root rot symptoms after 8 days.
Example 7
In the present embodiment, 100mL of nano silver sol (calculated by silver mass) with the concentration of 1000mg/L and 100mL of carboxymethyl cellulose solution with the concentration of 300mg/L are mixed, and thus the orchid disease control medicament containing 500mg/L of nano silver sol (calculated by silver mass) and 150mg/L of carboxymethyl cellulose is prepared.
Soaking the roots of orchid in the orchid disease control agent for 200min, taking out, draining, transplanting in a normal culture medium, and eliminating root rot symptoms after 4 days.
Example 8
In the embodiment, 100mL of nano silver sol with the concentration of 1000mg/L (calculated by silver mass) and 100mL of guar gum solution with the concentration of 100mg/L are mixed, and then the orchid disease control medicament containing 500mg/L of nano silver sol and 50mg/L of guar gum is prepared.
Soaking the roots of orchid in the orchid disease control agent for 200min, taking out, draining, transplanting in a normal culture medium, and eliminating root rot symptoms after 8 days.
Example 9
In the embodiment, 10mL of nano silver sol with the concentration of 800mg/L (calculated by silver mass) and 10mL of pectin solution with the concentration of 100mg/L are mixed, and then the orchid disease control medicament containing 400mg/L of nano silver sol and 50mg/L of pectin is prepared.
1mL of the above orchid disease control agent was applied to abnormal spot portions of leaves, and the whole orchid was subjected to ultraviolet light (light intensity 3.0X10) 16 photons/sec/cm 3 ) After 45min of irradiation, the leaf is placed in a shade at normal temperature, and abnormal spots of the leaf disappear after 6 days.
Example 10
In the present embodiment, 10mL of nano silver sol (calculated by silver mass) with the concentration of 800mg/L is mixed with 10mL of water-soluble starch solution with the concentration of 50mg/L, and thus the orchid disease control agent containing 400mg/L of nano silver sol (calculated by silver mass) and 25mg/L of water-soluble starch is prepared.
1mL of the above orchid disease control agent was applied to abnormal spot portions of leaves, and the whole orchid was subjected to ultraviolet light (light intensity 3.0X10) 17 photons/sec/cm 3 ) After 60min of irradiation, the leaf is placed in a shade at normal temperature, and abnormal spots of the leaf disappear after 5 days.
The above examples have been successfully implemented by the agricultural development limited company in the Wen Jiu Yu Fujian province, and each example has an effective rate of 99% or more by testing on 100 or more disease spots or sites. Meanwhile, the following two comparative examples were made, and 100 disease spots or sites were taken for each comparative example to conduct the test.
Comparative example 1: 10mL of the nanosilver sol having a concentration of 1000mg/L (based on the mass of silver) was diluted with ultrapure water to 20mL of the nanosilver sol having a concentration of 500 mg/L. Taking 0.5mL of the nano silver sol, smearing the nano silver sol on abnormal spot positions of orchid leaves, and placing the whole orchid in a shade place at normal temperature after the whole orchid is irradiated under sunlight for 60 min. After 4 days, the nano silver sol is observed to be incapable of adhering to the orchid leaves, so that the orchid leaves are extremely easy to fall on the ground, and abnormal spots of the leaves are not completely disappeared.
Comparative example 2: 10mL of a xanthan gum solution having a concentration of 1000mg/L was mixed, and diluted with ultrapure water to 20mL of a xanthan gum solution having a concentration of 500 mg/L. 1mL of the xanthan gum solution was applied to abnormal spots on leaves, and the whole orchid was subjected to ultraviolet light (light intensity of 2.0X10) 16 photons/sec/cm 3 ) After 60min of irradiation, the leaf is placed in a shade at normal temperature, and abnormal spots of the leaf remain after 5 days.
It should be noted that the parameters used in the preparation in the above examples are only for illustration and are not limiting to the application, and those skilled in the art can flexibly select the preparation parameters according to actual needs.
The nano silver sol can be one of all nano silver sol in the related art and a mixture thereof, and the polysaccharide can be one of all polysaccharide in the related art and a mixture thereof. The polysaccharides indicated in the present invention are only for the purpose of illustration and are not limiting on the kind of polysaccharide. In addition to the polysaccharides indicated in the present invention, the newly discovered polysaccharides can also be used in the preparation of the present invention.
Claims (10)
1. The orchid disease preventing and controlling agent includes nanometer silver sol and polysaccharide solution; wherein the concentration of the nano silver sol is 5mg/L-1000mg/L calculated by silver content, and the concentration of the polysaccharide is 10mg/L-10000mg/L.
2. The orchid disease control agent according to claim 1, wherein the particle diameter of the nano silver in the nano silver sol is 1 to 100nm.
3. An orchid disease control agent according to claim 1, wherein the polysaccharide comprises one or more of a natural polysaccharide or a natural polysaccharide degradation product.
4. An orchid disease control agent according to claim 3, wherein the natural polysaccharide comprises a mixture of one or more of the following polysaccharides: konjak glucomannan, chitosan, carrageenan, xanthan gum, algin, acacia gum, guar gum, water-soluble starch, carboxymethyl cellulose, soluble soybean polysaccharide, pectin and galactose mannans.
5. An orchid disease control agent according to claim 3, wherein the natural polysaccharide degradation products include: powdered natural polysaccharide degradation products and natural polysaccharide degradation product solutions dissolved in water and organic solvents.
6. An orchid disease control agent according to claim 3, wherein the natural polysaccharide degradation product comprises at least one of: natural polysaccharide hydrolysis products, natural polysaccharide alkaline hydrolysis products, natural polysaccharide polysaccharase hydrolysis products, natural polysaccharide products degraded by hydrogen peroxide, natural polysaccharide products degraded by gamma-rays, natural polysaccharide products degraded by ultrasonic waves and natural polysaccharide products degraded by nano metal photocatalysis.
7. Use of the orchid disease control agent according to any one of claims 1 to 6 for orchid disease control.
8. The use of claim 7, wherein the orchid disease comprises one or more of anthrax, southern blight, leaf blight, root rot, brown spot, and viral disease.
9. The method of using a chemical for controlling orchid diseases according to any one of claims 1 to 6, wherein (1) when the disease points are on leaves, the chemical is directly smeared on the disease points uniformly, and then the whole orchid is left under the sun light or left under the irradiation of ultraviolet light for a sufficient time; (2) When the disease points are at the rhizome, the root is completely soaked in the medicament for 10min-1000min, and then the root is taken out and filtered.
10. The method of using an orchid disease control agent according to claim 9, wherein the irradiation time under sunlight is 15min to 5d or the irradiation time under ultraviolet light is 5min to 3000min.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211647537.8A CN115997789A (en) | 2022-12-21 | 2022-12-21 | Orchid disease control medicament and application method thereof |
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| CN202211647537.8A CN115997789A (en) | 2022-12-21 | 2022-12-21 | Orchid disease control medicament and application method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117397698A (en) * | 2023-12-15 | 2024-01-16 | 中国农业大学 | Wood vinegar antibacterial agent containing nano silver particles and preparation method and application thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117397698A (en) * | 2023-12-15 | 2024-01-16 | 中国农业大学 | Wood vinegar antibacterial agent containing nano silver particles and preparation method and application thereof |
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