CN115943898A

CN115943898A - Depression mouse is device for rhythm disorder research

Info

Publication number: CN115943898A
Application number: CN202310213864.0A
Authority: CN
Inventors: 邢陈; 宋伦; 黄欣; 刘琨; 文青
Original assignee: Academy of Military Medical Sciences AMMS of PLA
Current assignee: Academy of Military Medical Sciences AMMS of PLA
Priority date: 2023-03-08
Filing date: 2023-03-08
Publication date: 2023-04-11
Anticipated expiration: 2043-03-08
Also published as: CN115943898B

Abstract

The invention belongs to the technical field of medical instruments, and particularly relates to a device for researching rhythm disorder of a depressed mouse, which is mainly used for: constructing a depression mouse model with circadian rhythm disorder; mainly solves the problems that: in the existing clinical experiment, the problem that depression is accompanied with circadian rhythm disorder is not considered in the construction process of the chronic restraint stress animal model; the bounding device which is provided with the bound mouse and can be separated from the box body is loaded by the box body with the LED light bars arranged inside, so that the induction of the circadian rhythm disorder of the experimental mouse in the process of carrying out a chronic bound mouse experiment is realized, and the effective construction of a disease mouse model with depression and concomitant circadian rhythm disorder is realized; the constraint of a plurality of experimental mice is realized, and the modeling efficiency of disease mice is improved.

Description

Depression mouse is device for rhythm disorder research

Technical Field

The invention belongs to the technical field of medical instruments, and particularly relates to a device for researching rhythm disorder of a depressed mouse.

Background

Depression is a global mental disease characterized by low mood, slow thinking, hypovolemia, impairment of cognitive function, etc.; depression has complex manifestations in physiological, neuroendocrine, behavioral and psychiatric aspects. Among them, circadian rhythm disorder is one of the most significant clinical symptoms in patients with depressive disorders. The rhythm disorder of depression patients is mainly reflected in the aspects of sleep-wake circadian rhythm disorder, circadian mood change, daily activity change, functional disorder of neuroendocrine system and the like.

In order to research the occurrence mechanism of depression, medicine intervention treatment, evaluation of pharmacological efficacy of medicines and the like, researchers establish various depression animal models; LPS injection induced depression and stress depression models are two most widely applied representative models, but the models do not take into account the problem that the clinical depression accompanies circadian rhythm disorder in the construction process; in addition, the chronic restraint stress model is also an important experimental paradigm for studying mental diseases represented by depression, but in the process of constructing the chronic restraint stress model, the problem of circadian rhythm disorder is also considered, so that designing an apparatus for effectively constructing a depression disease mouse model accompanied with circadian rhythm disorder is necessary.

The invention aims at the problems and provides a device for researching rhythm disorder of depressed mice.

Disclosure of Invention

In order to overcome the problems in the background art, the invention adopts the following technical scheme:

a device for studying rhythm disorders in depressed mice, comprising:

the experimental environment comprises a box body and a control device, wherein the box body is used for establishing an experimental environment, the box body is provided with a chamber which is used for placing an experimental animal and is provided with an opening, and a box door which can completely cover the opening of the chamber, and the box door is movably connected with the box body or detachably connected with the box body;

a binding device for binding the experimental animal, wherein the binding device is movably arranged in the chamber and can partially extend out of the chamber or completely separate from the chamber through the opening;

a light assembly for inducing circadian rhythm disturbances in experimental animals, the light assembly comprising: the light-emitting device comprises a light-emitting element and a control module for controlling the light-emitting element to start and stop, wherein the light-emitting element is arranged in the cavity; the control module is arranged on one side wall of the box body and is electrically connected with the light-emitting element.

In some embodiments of the present disclosure, the chamber has a top end surface and a bottom end surface, wherein the light emitting element is disposed on the top end surface, and the confinement device is radially translated on the bottom end surface.

Further, the bottom end face of the chamber is provided with a slide way, after the chamber door covers the opening of the chamber, the slide way is perpendicular to the chamber door, an inlet is formed in one end, close to the opening, of the slide way, and the binding device slides into the slide way through the inlet.

Optionally, the chute includes: the binding device is partially arranged in the first slide way and the second slide way, and partially extends out of the cavity or completely separates from the cavity through the first slide way and the second slide way.

In some embodiments of the present application, the restraint device comprises: dispose in the indoor support body of cavity for the constraint experimental animals and with the constraint subassembly that the support body is connected, wherein, the support body have the upper strata and with a plurality of spinal branch vaulting poles of upper strata connection, wherein, the upper strata has at least one and places the region, the constraint subassembly set up in place the region, a plurality of the one end of bracing piece connect in the land end, a plurality of the other end of bracing piece dispose in the slide.

When the placing areas are a plurality of, a partition board is arranged between every two adjacent placing areas, the partition board is perpendicular to the upper layer, and the binding assemblies are arranged between the adjacent partition boards.

Further, the binding assemblies can be separated from the placement area, and the number of the binding assemblies is the same as that of the placement area.

Optionally, the number of the binding assemblies is four, and the binding assemblies are arranged on the placing end face.

In some embodiments of the present application, the light emitting element comprises: the LED lamp strip is fixedly arranged on the top end face of the cavity, and one end of the LED lamp strip is electrically connected to the control module.

Optionally, the LED light bar is disposed along an edge of the top end surface of the cavity.

Further, the LED light bars are combined into a closed graph on the top end face of the cavity;

or, the LED light bar comprises: the lamp comprises a first lamp bar, a second lamp bar, a third lamp bar and a fourth lamp bar; the first light bar is parallel to the third light bar, the second light bar is parallel to the fourth light bar, the first light bar and the third light bar are perpendicular to the second light bar, and the first light bar, the second light bar, the third light bar and the fourth light bar are not connected with each other.

In some embodiments of the present application, the case further comprises: the exhaust fan is characterized in that the box body is provided with a top outer wall, and a through hole penetrating through the box wall of the box body is formed in the top outer wall; the exhaust fan is fixedly arranged in the through hole and partially extends into the cavity.

The invention has the beneficial effects that:

(1) Set up light-emitting component and set up the constraint device that can break away from in the cavity for in-process carrying out chronic constraint mouse experiment, the induced experiment mouse circadian rhythm disorder realizes effectively constructing the disease mouse model of depression concomitant circadian rhythm disorder.

(2) Realize tying many experimental mice through setting up a plurality of constraint subassemblies, improve the efficiency that the disease mouse was modeled, simultaneously, the contrast between the different disease mouse models of can also being convenient for.

Drawings

FIG. 1 is a schematic structural diagram of a case according to the present invention;

FIG. 2 is a schematic view of the structure inside the chamber of the present invention;

FIG. 3 is a schematic view of the structure of the chute of the present invention;

FIG. 4 is a schematic view of the restraining device and the interior of the case of the present invention;

FIG. 5 is a schematic view of a portion of the exit chamber of the restraint apparatus of the present invention;

FIG. 6 is a schematic view of a restraint device according to the present invention;

FIG. 7 is a schematic view of a frame structure according to the present invention;

FIG. 8 is a schematic view of the upper and lower layers of the present invention;

FIG. 9 is a schematic diagram of a tie-down plate structure according to the present invention;

FIG. 10 is a schematic view of the assembled tie plate of the present invention;

FIG. 11 is a schematic view of the notch structure of the tail fixing plate of the present invention;

in the figure, 1, a box body; 11. a box door; 12. a chamber; 121. an opening; 13. a slideway; 2. a frame body; 21. an upper layer; 211. a placement area; 212. a partition plate; 213. a steel wire; 22. a lower layer; 221. a tray; 23. a first lever; 24. a second lever; 25. a third lever; 26. a fourth bar; 27. a rotating wheel; 3. a binding plate; 31. a groove; 311. a first card slot; 312. a second card slot; 32. an interlayer; 321. a through hole; 33. a head fixing piece; 34. a tail fixing sheet; 341. a notch; 4. an LED light bar; 5. an exhaust fan; 51. a visor; 6. a placement body; 61. an operation key; 62. a display screen.

Detailed Description

The technical solutions in the embodiments of the present invention are clearly and completely described below by specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and other advantages and effects of the present invention can be easily understood by those skilled in the art from the disclosure of the present specification. The present invention can be implemented or applied by other different specific embodiments, and the features in the following embodiments and embodiments can be combined with each other without conflict, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.

Fig. 1 to 3, which illustrate the main technical content of the present embodiment, the present embodiment provides a device for studying rhythm disorder of depressed mice, comprising a housing 1 for constructing an experimental environment, the housing 1 is provided with a chamber 12 for placing experimental animals and having an opening 121, and a door 11 capable of completely covering the opening 121 of the chamber 12, wherein the door 11 is movably connected to the housing 1 or the door 11 is detachably connected to the housing 1;

a binding device for binding the experimental animal, wherein the binding device is movably arranged in the chamber 12 and can partially extend out of the chamber 12 from the opening 121 or completely separate from the chamber 12;

a light assembly for inducing circadian rhythm disturbances in experimental animals, the light assembly comprising: the light-emitting device comprises a light-emitting element and a control module for controlling the light-emitting element to start and stop, wherein the light-emitting element is arranged in a chamber 12; the control module is disposed on a sidewall of the box 1 and electrically connected to the light emitting element.

Wherein the experimental animal is experimental mouse.

Referring to fig. 2 to 3, in the present embodiment, the box body 1 has a first side wall and a second side wall, when the box door 11 is movably connected to the box body 1, a first groove is disposed on the first side wall, and a second groove is disposed on one side wall of the box door 11, wherein hinges are disposed in the first groove and the second groove, so that the box body 1 is hinged to the box door 11; the second groove is provided on a surface of the door 11 that contacts the cabinet 1 when covering the opening 121 of the chamber 12.

In addition, in order to ensure that the door 11 cannot be opened when covering the opening 121 of the chamber 12, in this embodiment, first magnetic stripes are disposed along at least two edges of the opening 121, a second magnetic stripe is disposed on one surface of the door 11 on which the second groove is disposed, and when the first magnetic stripe and the second magnetic stripe are close to each other, the first magnetic stripe and the second magnetic stripe attract each other, so that the door 11 always covers the opening 121 of the chamber 12; so that the chamber 12 of the box body 1 is always kept in a relatively closed and dark experimental environment; the use of light assemblies to control the lighting environment within the chamber 12 is facilitated.

Referring to fig. 1, in order to facilitate the opening of the door 11, a handle is disposed on a side wall of the door 11 opposite to the second groove, that is, in use, the door 11 is facilitated to be opened.

In other embodiments of the present application, not shown, the box door 11 and the box body 1 are separately disposed, and a first sliding slot and a second sliding slot are disposed on an end surface of the box body 1 having the opening 121, wherein the first sliding slot is parallel to the second sliding slot, and two ends of the first sliding slot and the second sliding slot are not blocked, and the box door 11 enters the first sliding slot and the second sliding slot at one end of the first sliding slot and the second sliding slot, and slides towards the other end of the first sliding slot and the other end of the second sliding slot until the opening 121 is completely blocked.

Referring to fig. 1-2, in the present embodiment, the housing 1 is rectangular, and the chamber 12 is also rectangular in shape; the chamber 12 has a top end surface and a bottom end surface, the light emitting element for creating a bright environment is disposed on the top end surface of the chamber 12, and the binding device is spaced from the top end surface of the chamber 12, so that the light emitting element can uniformly irradiate the binding device; the confinement device is capable of translating on the bottom end face of the chamber 12; it is convenient to place, take and to move the restraint device out of the chamber 12 to observe the experimental mouse before and during the experiment.

Referring to fig. 2-3, in the present embodiment, the binding device is disposed in the chamber 12 to be capable of moving only in one reciprocating direction, and a slide 13 is disposed on a bottom end surface of the chamber 12, wherein after the door 11 covers the opening 121 of the chamber 12, the slide 13 is perpendicular to the door 11, and an end of the slide 13 close to the opening 121 is disposed as an entrance, from which the binding device slides into the slide 13, and more specifically, the slide 13 includes: the binding device is partially arranged in the first slideway and the second slideway, so that the binding device partially extends out of the chamber 12 or completely separates out of the chamber 12 through the first slideway and the second slideway; meanwhile, the restraint device can be conveniently limited in position in the box body 1, so that experimental mice in different batches can be subjected to molding experiments under the same illumination position.

Referring to fig. 2 and 4-8, in the present embodiment, a restraint device is provided, which includes: the rack body 2 is arranged in the cavity 12, the binding assembly is used for binding the experimental mouse and is connected with the rack body 2, the rack body 2 is provided with an upper layer 21 and a lower layer 22 which are parallel to each other and a plurality of support rods for connecting the upper layer 21 and the lower layer 22, and the binding assembly is detachably connected with the upper layer 21; wherein, the bracing piece includes: a first bar 23, a second bar 24, a third bar 25, a fourth bar 26; the first rod 23, the second rod 24, the third rod 25 and the fourth rod 26 connect the corners of the upper layer 21 and the lower layer 22, and the first rod 23, the second rod 24, the third rod 25 and the fourth rod 26 are all perpendicular to the upper layer 21; more specifically, the upper layer 21 and the lower layer 22 each have: a first corner, a second corner, a third corner, and a fourth corner; a first bar 23 connecting a first corner of the upper layer 21 with a first corner of the lower layer 22, a second bar 24 connecting the upper layer 21 with a second corner of the lower layer 22, a third bar 25 connecting the upper layer 21 with a third corner of the lower layer 22, a fourth bar 26 connecting the upper layer 21 with a fourth corner of the lower layer 22;

optionally, the other ends of the first rod 23, the second rod 24, the third rod 25, and the fourth rod 26 are all provided with a runner 27, wherein the width of the runner 27 is the same as the width of the slide 13.

Referring to fig. 5 to 8, in the present embodiment, an upper layer 21 is disposed, the upper layer 21 includes a plurality of placing regions 211, a partition 212 is disposed between each two adjacent placing regions 211, the partition 212 is perpendicular to the lower layer 22, and a binding assembly is disposed between the adjacent partitions 212, wherein a plurality of steel wires 213 are arranged in the placing regions 211, and a distance exists between each two adjacent steel wires 213; make the object of upper strata 21 can drop to lower floor 22, realize promptly in the mouse experiment process that the produced excrement of experimental mouse that upper strata 21 was placed can drop in lower floor 22 through the blank region between adjacent steel wire 213 to the centralized processing to experimental mouse excrement.

Referring to fig. 5 to 7, more specifically, the number of the placement areas 211 is set to four; wherein, the number of the partition plates 212 is always one more than that of the placing areas 211; the number of tie down assemblies is the same as the number of placement areas 211; the batch constraint on experimental mice is realized, and after the model making of the animal model is finished, different mice successfully made can also be compared in behaviors.

And the lower layer 22 includes: the frame and the tray 221 that is used for collecting experimental mouse excrement, wherein, the frame has inserted hole and border portion, and the one end of tray 221 gets into in the frame from the inserted hole, and the edge overlap joint of tray 221 in the border portion of frame.

The tray 221 has a recessed area into which excreta fall after the mice bound by the upper layer 21 excrete; preventing mouse fluid waste from flowing out of the tray 221.

Referring to fig. 4-7, in other embodiments of the present application, the frame body 2 may also be composed of only the upper layer 21, and the first, second, third and

fourth rods

23, 24, 25 and 26, wherein one end of the first rod 23 is connected to the first corner of the upper layer 21, one end of the second rod 24 is connected to the second corner of the upper layer 21, one end of the third rod 25 is connected to the third corner of the upper layer 21, and one end of the fourth rod 26 is connected to the fourth corner of the upper layer 21; and the other ends of the first rod 23, the second rod 24, the third rod 25, and the fourth rod 26 are slidable in the sliding direction.

Referring to fig. 2 and 5, in another embodiment of the present application, a receiving groove is disposed at a bottom end surface of the chamber 12, the tray 221 is disposed in the receiving groove, and the tray 221 can be separated from the receiving groove.

Referring to fig. 6-10, in this embodiment, a leasing assembly is provided, the leasing assembly comprising: a binding plate 3, a head fixing sheet 33 and a tail fixing sheet 34; the binding plate 3 is provided with a first outer wall, a plurality of grooves 31 are arranged on the first outer wall, an interlayer 32 is arranged to shield parts of the grooves 31, a head fixing piece 33 is inserted into one end of each groove 31, a tail fixing piece 34 is inserted into the other end of each groove 31, and the head fixing piece 33 and the tail fixing piece 34 can be separated from the grooves 31; more specifically, the groove 31 has a first end and a second end, and a first card slot 311 group including a plurality of first card slots 311 and a second card slot 312 group including a plurality of second card slots 312 are disposed on an inner wall of the groove 31, wherein the first card slots 311 are arranged in a direction from the first end of the groove 31 to the second end of the groove 31, and the second card slots 312 and the first end of the groove 31 are arranged in a direction from the first end of the groove 31 to the second end of the groove 31; when the head fixing piece 33 is inserted into the first card slot 311, the tail fixing piece 34 is inserted into the second card slot 312; thereby limiting the moving range of the experimental mouse.

Referring to fig. 5 to 6, after the binding plate 3 is placed in the placement region 211, the central line of the groove 31 thereof is perpendicular to the upper layer 21, so that the experimental mouse assumes an upright state during the experiment with the head of the experimental mouse directed toward the light emitting element to induce the disturbance of the circadian rhythm of the experimental mouse or reverse the cognition of the experimental mouse on the circadian rhythm; the device is convenient for the natural downward flow of the excrement of the mouse, and in addition, the binding plate 3 can be turned upside down according to the experimental requirement, so that the mouse can be placed upside down.

Referring to fig. 11, in order to define the tail of the experimental mouse, in this embodiment, a notch 341 is reserved in the tail fixing plate 34, when in use, the tail of the experimental mouse is limited in the notch 341, and meanwhile, the notch 341 is disposed to facilitate the natural downward flow of the excrement of the mouse.

Referring to fig. 6 to 7, the number of the binding plates 3 and the number of the placing areas 211 are 4, and the number of the grooves 31 formed on the first outer wall of the binding plates 3 is 10, so that a plurality of mice can be bound at one time during the experiment, and the experiment mice are induced by circadian rhythm disorder in batch.

Referring to fig. 9-10, more specifically, the first card slot 311 group includes first card slots 311 more than one and less than ten, the number of the first card slots 311 is preferably four, and the second card slot 312 group includes second card slots 312 more than one and less than ten, the number of the second card slots 312 is also preferably four; when the head fixing piece 33 and the tail fixing piece 34 are located in the first clamping groove 311 and the second clamping groove 312 at different positions, the distance between the head fixing piece 33 and the tail fixing piece 34 is different, so that the front and back movement range of the experimental mouse can be limited to be the minimum according to the body type of the experimental mouse.

Referring to fig. 6 and 9, in addition, in order to prevent the mouse from suffocation death between the partition 32 and the groove 31 for a long time, a plurality of through holes 321 leading to the groove 31 are arranged on the outer wall of the partition 32, and the groove 31 is provided with at least one corresponding through hole 321.

The length and width of the partition 32 are set, wherein the length of the partition 32 is the same as the length of the first outer wall of the binding plate 3, and the width of the partition 32 is smaller than the width of the binding plate 3.

Referring to fig. 6 to 7 and 9 to 10, in order to ensure the light transmittance of the binding plate 3, the partition layer 32, the head fixing piece 33, the tail fixing piece 34 and the partition plate 212, the binding plate 3, the partition layer 32, the head fixing piece 33, the tail fixing piece 34 and the partition plate 212 are made of polypropylene, so that the light receiving degree of the experimental mouse can be ensured while the weight is reduced.

Referring to fig. 2, in the present embodiment, the light emitting element includes at least one LED light bar 4, wherein the LED light bar 4 is fixedly disposed on the top end surface of the chamber 12, and the LED light bar 4 is electrically connected to the control module, so as to illuminate the area inside the chamber 12 through the LED light bar 4, so as to simulate the experimental environment during the daytime.

When the number of the LED light bars 4 is one, the LED light bars 4 are combined into a closed figure, preferably a rectangle, on the top end face of the cavity 12, and when the LED light bars are used, all-around shining can be carried out on bound experimental mice, so that the illumination intensity of the experimental mice bound in batches is the same; more preferably, the LED light bar 4 is disposed along the edge of the top end surface of the cavity 12.

When the LED light bar 4 is provided as a plurality of bars, the LED light bar includes: the lamp comprises a first lamp bar, a second lamp bar, a third lamp bar and a fourth lamp bar; the first light bar is parallel to the third light bar, the second light bar is parallel to the fourth light bar, the first light bar is perpendicular to the second light bar, and the first light bar, the second light bar, the third light bar and the fourth light bar are not connected with each other.

Referring to fig. 1-3, in order to keep the air in the chamber 12 of the box 1 circulating during the experiment, in this embodiment, a through hole communicating with the chamber 12 is formed on the outer wall of the top of the box 1, and an exhaust fan 5 is disposed in the through hole; in addition, the exhaust fan 5 is electrically connected with the control module; more specifically, the exhaust fan 5 adopts a low-noise exhaust fan 5 and is fixedly arranged in the through hole; therefore, the air circulation in the box body 1 is ensured, the air in the chamber 12 is novel, and the ammonia concentration is prevented from exceeding 20 ppm.

In addition, on the basis that the box body 1 is provided with the exhaust fan 5, external light is prevented from irradiating the cavity 12 through a gap of the exhaust fan 5, so that failure of modeling of circadian rhythm disorder of the experimental mouse is caused, therefore, the light shielding plate 51 is arranged on the top end surface of the cavity 12, wherein a connecting rod is arranged on the same outer wall of the light shielding plate 51, the light shielding plate 51 is connected with the top end surface of the cavity 12 through the connecting rod, a distance is reserved between the light shielding plate 51 and the top end surface of the cavity 12, and the light shielding plate 51 is prevented from being blocked on the exhaust fan 5; more preferably, the length and width of the light shielding plate 51 are greater than those of the through opening; when the device is used, the direct-emitting point of the external light rays penetrating through the gap of the exhaust fan 5 always falls on the light shielding plate 51, so that the external light rays are prevented from damaging the night experiment environment simulated by the chamber 12.

Referring to fig. 1 to 3, in the present embodiment, the control module controls the lighting time of the LED light bar 4 and the illumination time of the experimental mouse; its control module sets up in the top outer wall of box 1, and wherein, control module includes: an operating key 61, a display screen 62, a power supply module and a control main board; arranging a placing body 6 on the top end face of the cavity 12, arranging placing grooves in the outer wall of the top of the box body 1 and the placing body 6, arranging a power supply module and a control mainboard in the placing grooves, arranging a top plate to seal the placing grooves, arranging an operation key 61 and a display screen 62 on the top plate, and electrically connecting the operation key 61 and the display screen 62 to the control mainboard; when the LED lamp strip is used, the lighting time and the illumination time of the LED lamp strip 4 are set by the operation keys 61 and displayed on the display screen 62, and the power supply module supplies power to the LED lamp strip 4, the control main board, the operation keys 61, the display screen 62 and the exhaust fan 5.

In addition, the power supply module includes: a storage battery and a charging interface; the battery sets up in the standing groove, and the interface that charges sets up in any lateral wall of box 1, and the interface that charges is in battery electric connection.

The power module may further include: a battery jar and a cover plate are arranged on any side wall of the box body 1; the battery jar is used for placing a battery, the cover plate is used for sealing the battery jar, and the cover plate can be detached from the battery jar.

The use method of the invention comprises the following steps:

when the mouse trap is used, the box door is opened, the frame body is partially or completely separated from the cavity, then the binding plate is taken down from the placing area, a mouse used for an experiment is placed in each groove of the binding plate, the head fixing piece and the tail fixing piece are inserted into the first clamping groove and the second clamping groove according to the body type of the mouse, the front and back moving range of the mouse is limited to the minimum, then the binding plate loaded with the mouse is placed in the placing area of the frame body, the frame body is placed in the box body, then the box door is closed, and the lighting time and the illumination time of the LED lamp strip are set through the operation keys; meanwhile, the exhaust fan is always in a working state.

The above description of the embodiments is only intended for the understanding of the present invention. It should be noted that modifications can be made to the invention without departing from the principle of the invention, which will fall within the scope of the appended claims.

Claims

1. A device for studying rhythm disorder of depressed mice, comprising:

the experimental environment is established in the box body, the box body is provided with a chamber which is used for placing experimental animals and is provided with an opening, and a box door which can completely cover the opening of the chamber, wherein the box door is movably connected with the box body or detachably connected with the box body;

2. The device of claim 1, wherein the chamber has a top surface and a bottom surface, wherein the light emitting element is disposed on the top surface and the restraining device is radially translatable on the bottom surface.

3. The device for studying rhythm disorder of depressed mice as claimed in claim 2, wherein said bottom end surface of said chamber is provided with a slide way, said slide way is perpendicular to said door after said door covers said opening of said chamber, and one end of said slide way near said opening is provided with an access port, and said binding device is slid into said slide way through said access port.

4. The device for studying rhythm disorder of depressed mouse according to claim 3, wherein said restraint means comprises: dispose in the indoor support body of cavity for the constraint experimental animals and with the constraint subassembly that the support body is connected, wherein, the support body have the upper strata and with a plurality of spinal branch vaulting poles of upper strata connection, wherein, the upper strata has at least one and places the region, the constraint subassembly set up in place the region, a plurality of the one end of bracing piece connect in the land end, a plurality of the other end of bracing piece dispose in the slide.

5. The device for studying rhythm disorder of depressed mouse as claimed in claim 4, wherein when said placement areas are plural, a partition is provided between each adjacent placement area, the partition is perpendicular to the upper layer, and the binding member is provided between the adjacent partitions.

6. The device for studying depressive mouse rhythm disorder according to claim 4, wherein the restraint members are separable from the placement regions, and the number of the restraint members is the same as the number of the placement regions.

7. The device for studying rhythm disorder of depressed mouse of claim 4, the restraint member comprising: a binding plate, a head fixing plate and a tail fixing plate; wherein, the constraint board has first outer wall, and first outer wall is arranged and is provided with a plurality of recess to set up the interlayer with the sheltering from of a plurality of groove part, the head stationary blade is pegged graft in the one end of recess, the afterbody stationary blade is pegged graft in the other end of recess, and head stationary blade and afterbody stationary blade can break away from in the recess.

8. The device for studying rhythm disorder of depressed mouse according to claim 2, wherein said light emitting element comprises: the LED lamp strip is fixedly arranged on the top end face of the cavity, and one end of the LED lamp strip is electrically connected to the control module.

9. The device for studying rhythm disorder of depressed mouse of claim 8, wherein said LED light bars are combined in a closed figure on said top end surface of said chamber;

10. The device for studying rhythm disorder of depressed mouse of claim 1, wherein the case further comprises: the exhaust fan is characterized in that the box body is provided with a top outer wall, and a through hole penetrating through the box wall of the box body is formed in the top outer wall; the exhaust fan is fixedly arranged in the through hole and partially extends into the cavity.