CN115943898B

CN115943898B - Device for researching rhythm disorder of depressed mice

Info

Publication number: CN115943898B
Application number: CN202310213864.0A
Authority: CN
Inventors: 邢陈; 宋伦; 黄欣; 刘琨; 文青
Original assignee: Academy of Military Medical Sciences AMMS of PLA
Current assignee: Academy of Military Medical Sciences AMMS of PLA
Priority date: 2023-03-08
Filing date: 2023-03-08
Publication date: 2023-05-30
Anticipated expiration: 2043-03-08
Also published as: CN115943898A

Abstract

The invention belongs to the technical field of medical equipment, and particularly relates to a device for researching rhythm disorder of a depressed mouse, which is mainly used for: constructing a depression mouse model of circadian rhythm disorder; mainly solves the following problems: in the current clinical experiment, the problem that depression is accompanied with circadian rhythm disorder is not considered in the process of constructing a chronic constraint stress object model; through setting up the box with LED light bar inside, load the bound mouse and can break away from the binding device of the box, have realized carrying on the course of the experiment of the mouse of chronic constraint, induce the circadian rhythm disorder of the experimental mouse, realize constructing the depression and concomitantly the disease mouse model of circadian rhythm disorder effectively; the method realizes the restraint of a plurality of experimental mice and improves the modeling efficiency of the disease mice.

Description

Device for researching rhythm disorder of depressed mice

Technical Field

The invention belongs to the technical field of medical appliances, and particularly relates to a device for researching rhythm disorder of a depressed mouse.

Background

Depression is a global mental disorder characterized by low mood, slow thinking, hypovolemia, impaired cognitive function, etc.; depression has complex manifestations in physiological, neuroendocrine, behavioral and mental aspects. Among them, circadian rhythm disorder is one of the most remarkable clinical symptoms of patients with depression. The rhythm disorder of depressed patients is mainly reflected in the aspects of sleep wake circadian rhythm disorder, circadian emotion change, daily activity change, functional abnormality of neuroendocrine system and the like.

In order to research the occurrence mechanism of depression, the intervention treatment of drugs, the evaluation of pharmacological effects of the drugs and the like, researchers establish a plurality of animal models of depression; LPS injection induced depression and stress depression models are two representative models with the most extensive application, but the models do not give consideration to the problems of clinical depression accompanied by circadian rhythm disorder in the construction process; in addition, the chronic constraint stress model is also an important experimental model for researching mental diseases represented by depression, but the same is also a problem of giving attention to circadian rhythm disturbance in the process of constructing the chronic constraint stress model, so that an instrument for effectively constructing a depression disease mouse model accompanied with circadian rhythm disturbance is very necessary.

The present invention addresses the above-described problems and provides a device for studying rhythm disorders in depressed mice.

Disclosure of Invention

In order to overcome the problems in the background art, the invention adopts the following technical scheme:

a device for studying a depressive mouse rhythm disorder, comprising:

the box body is used for constructing an experimental environment, is provided with a chamber which is used for placing experimental animals and is provided with an opening and a box door which can completely cover the opening of the chamber, wherein the box door is movably connected with the box body or detachably connected with the box body;

the restraint device is used for restraining the experimental animal, is movably arranged in the cavity and can be separated from the cavity by the opening part or all of the opening part;

a light assembly for inducing circadian rhythm disorders in an experimental animal, the light assembly comprising: the light-emitting device comprises a light-emitting element and a control module for controlling the start and stop of the light-emitting element, wherein the light-emitting element is arranged in the cavity; the control module is arranged on one side wall of the box body and is electrically connected with the light-emitting element.

In some embodiments of the present application, the chamber has a top end face and a bottom end face, wherein the light emitting element is disposed at the top end face and the binding device is radially translated at the bottom end face.

Further, the bottom end face of the chamber is provided with a slide way, the slide way is perpendicular to the chamber door after the chamber door covers the opening of the chamber, one end, close to the opening, of the slide way is provided with an inlet, and the restraint device slides into the slide way from the inlet.

Optionally, the slideway comprises: the binding device comprises a first slideway and a second slideway, wherein the binding device part is arranged in the first slideway and the second slideway, and partially protrudes out of the chamber or completely leaves the chamber through the first slideway and the second slideway.

In some embodiments of the present application, the tie down apparatus comprises: the frame body is arranged in the cavity and is used for binding experimental animals and is connected with a binding assembly of the frame body, wherein the frame body is provided with an upper layer and a plurality of supporting rods connected with the upper layer, the upper layer is provided with at least one placement area, the binding assembly is arranged in the placement area, one ends of the supporting rods are connected with the connecting end face, and the other ends of the supporting rods are arranged in the slideway.

When the placing areas are a plurality of, each adjacent placing area is provided with a partition board, the partition boards are perpendicular to the upper layer, and the binding assembly is arranged between the adjacent partition boards.

Further, the tie down assemblies can be separated from the placement areas, and the number of tie down assemblies is the same as the number of placement areas.

Optionally, the number of the binding assemblies is four, and the binding assemblies are arranged on the placing end face in an arrayed mode.

In some embodiments of the present application, the light emitting element includes: the LED lamp strip is fixedly arranged on the top end face of the cavity, and one end of the LED lamp strip is electrically connected with the control module.

Optionally, the LED light bar is disposed along an edge of the top end surface of the chamber.

Further, the LED light bars are combined into a closed pattern on the top end face of the cavity;

alternatively, the LED light bar includes: the first light bar, the second light bar, the third light bar and the fourth light bar; the first light bar is parallel to the third light bar, the second light bar is parallel to the fourth light bar, the first light bar is perpendicular to the third light bar, and the first light bar, the second light bar, the third light bar and the fourth light bar are not connected with each other.

In some embodiments of the present application, the case further comprises: the exhaust fan is characterized in that the box body is provided with a top outer wall, and a through hole penetrating through the box wall of the box body is formed in the top outer wall; the exhaust fan is fixedly arranged at the through hole and partially extends into the cavity.

The invention has the beneficial effects that:

(1) The luminous component is arranged in the cavity and the restraint device which can be separated from the cavity is arranged, so that the circadian rhythm disorder of the experimental mouse is induced in the process of carrying out the experiment of the chronic restraint mouse, and the effect of effectively constructing a disease mouse model with circadian rhythm disorder accompanied by depression is realized.

(2) Through setting up a plurality of constraint subassembly realization and constraint many experimental mice, improve the efficiency of disease mouse modeling, simultaneously, can also be convenient for the contrast between the different disease mouse models.

Drawings

FIG. 1 is a schematic diagram of the structure of the box of the present invention;

FIG. 2 is a schematic view of the internal structure of the chamber of the present invention;

FIG. 3 is a schematic view of a slide structure according to the present invention;

FIG. 4 is a schematic view of the internal structure of the restraint device and the case of the present invention;

FIG. 5 is a schematic view of the part of the restraint device of the present invention which extends out of the cavity;

FIG. 6 is a schematic view of the structure of the restraint device of the present invention;

FIG. 7 is a schematic view of the structure of the frame of the present invention;

FIG. 8 is a schematic diagram of the upper and lower layers of the present invention;

FIG. 9 is a schematic view of a tie-down plate structure of the present invention;

FIG. 10 is a schematic view of the assembled structure of the tie down plate of the present invention;

FIG. 11 is a schematic view of a notch structure of a tail anchor tab according to the present invention;

in the figure, 1, a box body; 11. a door; 12. a chamber; 121. an opening; 13. a slideway; 2. a frame body; 21. an upper layer; 211. a placement area; 212. a partition plate; 213. a steel wire; 22. a lower layer; 221. a tray; 23. a first lever; 24. a second lever; 25. a third lever; 26. a fourth lever; 27. a rotating wheel; 3. a tie-down plate; 31. a groove; 311. a first clamping groove; 312. a second clamping groove; 32. an interlayer; 321. a through hole; 33. a head fixing piece; 34. a tail fixing piece; 341. a notch; 4. LED light bar; 5. an exhaust fan; 51. a light shielding plate; 6. a placement body; 61. an operation key; 62. and a display screen.

Detailed Description

The following detailed description of the embodiments of the present invention will be made more apparent to those skilled in the art from the following detailed description, in which the invention is embodied in several, but not all, embodiments of the invention. The invention may be embodied or applied in other specific forms and features of the following examples and examples may be combined with each other without conflict, all other examples being contemplated by those of ordinary skill in the art without undue burden from the present disclosure, based on the examples of the invention.

Fig. 1 to 3 show the main technical content of the present embodiment, and the present embodiment provides a device for studying circadian disorders of depressed mice, which comprises a case 1 for constructing an experimental environment, the case 1 being provided with a chamber 12 for placing experimental animals and having an opening 121, and a case door 11 capable of completely covering the opening 121 of the chamber 12, wherein the case door 11 is movably connected to the case 1 or the case door 11 is detachably connected to the case 1;

a restraint device for restraining the experimental animal, wherein the restraint device is movably arranged in the chamber 12, and the restraint device can partially extend out of the chamber 12 or completely extend out of the chamber 12 through the opening 121;

a light assembly for inducing circadian rhythm disorder in an experimental animal, the light assembly comprising: a light emitting element and a control module for controlling the start and stop of the light emitting element, wherein the light emitting element is arranged in the chamber 12; the control module is arranged on one side wall of the box body 1 and is electrically connected with the light-emitting element.

Wherein the experimental animal is an experimental mouse.

Referring to fig. 2-3, in the present embodiment, the case 1 has a first side wall and a second side wall, when the case door 11 is movably connected to the case 1, a first groove is disposed on the first side wall, and a second groove is disposed on one side wall of the case door 11, wherein hinges are disposed in the first groove and the second groove, so that the case 1 is hinged to the case door 11; the second groove is provided on a surface of the door 11 that contacts the case 1 when covering the opening 121 of the chamber 12.

In addition, in order to ensure that the door 11 is not opened when covering the opening 121 of the chamber 12, in this embodiment, a first magnetic stripe is disposed along at least two edges of the opening 121, a second magnetic stripe is disposed on one surface of the door 11 where the second groove is disposed, and when the first magnetic stripe is close to the second magnetic stripe, the first magnetic stripe and the second magnetic stripe are attracted to each other, so that the door 11 always covers the opening 121 of the chamber 12; so that the chamber 12 of the box body 1 is always kept in a relatively closed and dark experimental environment; the use of a light assembly to control the lighting environment within the chamber 12 is facilitated.

Referring to fig. 1, in order to facilitate the opening of the door 11, a handle is provided on a side wall of the door 11 opposite to the second groove, that is, in use, to facilitate the opening of the door 11.

In other embodiments of the present application, not shown, the box door 11 and the box 1 are detachably disposed, and then a first chute and a second chute are disposed on an end surface of the box 1 with the opening 121, wherein the first chute is parallel to the second chute, two ends of the first chute and two ends of the second chute are not plugged, and the box door 11 enters the first chute and the second chute at one end of the first chute and one end of the second chute and slides towards the other ends of the first chute and the second chute until the opening 121 is completely plugged.

Referring to fig. 1-2, in this embodiment, the housing 1 is rectangular, as is the chamber 12; wherein the chamber 12 has a top end surface and a bottom end surface, the light emitting element for creating a bright environment is arranged on the top end surface of the chamber 12, and the binding device is spaced from the top end surface of the chamber 12, so that the light emitting element can uniformly irradiate the binding device; the tie down device is capable of translating on the bottom end face of the chamber 12; the experimental mice are conveniently placed, taken and horizontally moved out of the chamber 12 before and during the experiment, and are observed.

Referring to fig. 2-3, in the present embodiment, the restraint device is disposed in the chamber 12 so as to be capable of moving only in one reciprocating direction, and a slide 13 is disposed on the bottom end surface of the chamber 12, wherein, after the door 11 covers the opening 121 of the chamber 12, the slide 13 is perpendicular to the door 11, and one end of the slide 13 near the opening 121 is disposed as an entrance, and the restraint device slides into the slide 13 from the entrance, more specifically, the slide 13 includes: the first slide way and the second slide way are partially arranged in the first slide way and the second slide way, so that the restraint device partially protrudes out of the cavity 12 or completely leaves out of the cavity 12 through the first slide way and the second slide way; meanwhile, the limiting device can be conveniently limited in the box body 1, so that experimental mice in different batches can be subjected to modeling experiments at the same illumination position.

Referring to fig. 2 and 4-8, in this embodiment, the restraint device is provided, which includes: the device comprises a frame body 2 arranged in a chamber 12, a binding component used for binding an experimental mouse and connected with the frame body 2, wherein the frame body 2 is provided with an upper layer 21, a lower layer 22 and a plurality of supporting rods for connecting the upper layer 21 with the lower layer 22 which are parallel, and the binding component is detachably connected with the upper layer 21; wherein, the bracing piece includes: a first lever 23, a second lever 24, a third lever 25, a fourth lever 26; the first rod 23, the second rod 24, the third rod 25 and the fourth rod 26 connect the corners of the upper layer 21 and the lower layer 22, and the first rod 23, the second rod 24, the third rod 25 and the fourth rod 26 are perpendicular to the upper layer 21; more specifically, the upper layer 21 and the lower layer 22 each have: a first corner, a second corner, a third corner, a fourth corner; the first rod 23 connects the upper layer 21 with the first corner of the lower layer 22, the second rod 24 connects the upper layer 21 with the second corner of the lower layer 22, the third rod 25 connects the upper layer 21 with the third corner of the lower layer 22, and the fourth rod 26 connects the upper layer 21 with the fourth corner of the lower layer 22;

optionally, a rotating wheel 27 is disposed at the other ends of the first rod 23, the second rod 24, the third rod 25 and the fourth rod 26, wherein the width of the rotating wheel 27 is the same as the width of the slideway 13.

Referring to fig. 5 to 8, in the present embodiment, the upper layer 21 is provided, the upper layer 21 includes a plurality of placement areas 211, a partition 212 is provided between each adjacent placement area 211, the partition 212 is perpendicular to the lower layer 22, and the restraint assembly is provided between the adjacent partition 212, wherein the placement areas 211 are arranged with a plurality of steel wires 213, and a distance exists between each two adjacent steel wires 213; so that the object of the upper layer 21 can drop to the lower layer 22, namely, the excrement generated by the experimental mice placed on the upper layer 21 can drop to the lower layer 22 through the blank area between the adjacent steel wires 213 in the experimental mouse experiment process, so as to intensively treat the excrement of the experimental mice.

Referring to fig. 5 to 7, more specifically, the number of the placement areas 211 is set to four; wherein the number of the partition plates 212 is always one more than the number of the placement areas 211; the number of tie down assemblies is the same as the number of placement areas 211; the experimental mice are bound in batches, and after the modeling of the animal model is completed, different successfully modeled mice can be subjected to comparison between behaviors.

While the lower layer 22 comprises: the device comprises a frame and a tray 221 for collecting excretions of experimental mice, wherein the frame is provided with an insertion opening and a rim part, one end of the tray 221 enters the frame from the insertion opening, and the rim of the tray 221 is lapped on the rim part of the frame.

The tray 221 has a concave area into which excreta falls after excreta of the mice bound by the upper layer 21; preventing the mouse fluid waste from flowing out of the tray 221.

Referring to fig. 4-7, in other embodiments of the present application, the frame 2 may also be composed of only the upper layer 21 and the first rod 23, the second rod 24, the third rod 25, and the fourth rod 26, wherein one end of the first rod 23 is connected to the first corner of the upper layer 21, one end of the second rod 24 is connected to the second corner of the upper layer 21, one end of the third rod 25 is connected to the third corner of the upper layer 21, and one end of the fourth rod 26 is connected to the fourth corner of the upper layer 21; and the other ends of the first, second, third, and

fourth levers

23, 24, 25, and 26 are slidable in the slide.

Referring to fig. 2 and 5, in other embodiments of the present application, a receiving groove is provided with the bottom end surface of the chamber 12, the tray 221 is provided in the receiving groove, and the tray 221 can be separated from the receiving groove.

Referring to fig. 6-10, in this embodiment, a restraint assembly is provided, the restraint assembly comprising: a binding plate 3, a head fixing piece 33 and a tail fixing piece 34; the binding plate 3 has a first outer wall, a plurality of grooves 31 are arranged on the first outer wall, a partition layer 32 is arranged to shield a part of the plurality of grooves 31, a head fixing piece 33 is inserted into one end of the grooves 31, a tail fixing piece 34 is inserted into the other end of the grooves 31, and the head fixing piece 33 and the tail fixing piece 34 can be separated from the grooves 31; more specifically, the groove 31 has a first end and a second end, and the inner wall of the groove 31 is provided with a first clamping groove 311 group including a plurality of first clamping grooves 311 and a second clamping groove 312 group including a plurality of second clamping grooves 312, wherein the first clamping groove 311 group is arranged in a direction from the first end of the groove 31 to the second end of the groove 31, and the second clamping groove 312 group is arranged in a direction from the first end of the groove 31 to the second end of the groove 31; when the head fixing piece 33 is inserted into the first clamping groove 311, the tail fixing piece 34 is inserted into the second clamping groove 312; thereby limiting the range of motion of the experimental mice.

Referring to fig. 5 to 6, after the restraint plate 3 is placed in the placement area 211, the center line of the groove 31 thereof is perpendicular to the upper layer 21, so that the experimental mouse assumes an upright state during the experiment, and the head of the experimental mouse is directed to the light emitting element, to induce the disturbance of circadian rhythm of the experimental mouse or reverse the cognition of the experimental mouse to the circadian rhythm; the natural downflow of the excreta of the mice is convenient, and in addition, the restraint plate 3 can be turned upside down according to the experimental requirement, so that the mice can be placed upside down.

Referring to fig. 11, in order to limit the tail of the experimental mouse, in this embodiment, a notch 341 is reserved in the tail fixing piece 34, and when in use, the tail of the experimental mouse is limited in the notch 341, and meanwhile, the setting of the notch 341 can also facilitate the natural downflow of the excreta of the mouse.

Referring to fig. 6 to 7, the number of the restraint plates 3 and the number of the placement areas 211 are 4 as well, and the number of the grooves 31 provided in the first outer wall of the restraint plates 3 is 10, so that a plurality of mice can be restrained at one time at the time of the experiment, so that the experiment mice perform the induction of circadian rhythm disorder in batches.

Referring to fig. 9 to 10, more specifically, the first set of slots 311 includes a number of first slots 311 greater than one and less than ten, the number of first slots 311 is preferably four, and the second set of slots 312 includes a number of second slots 312 greater than one and less than ten, the number of second slots 312 is also preferably four; when the head fixing piece 33 and the tail fixing piece 34 are in the first clamping groove 311 and the second clamping groove 312 at different positions, the distances between the head fixing piece 33 and the tail fixing piece 34 are different, so that the movement range of the experimental mouse can be limited to be minimum according to the body shape of the experimental mouse.

Referring to fig. 6 and 9, in order to prevent mice from being in choking death between the interlayer 32 and the groove 31 for a long time, a plurality of through holes 321 leading to the groove 31 are provided on the outer wall of the interlayer 32, and the groove 31 has at least one corresponding through hole 321.

The length and the width of the spacer layer 32 are set, wherein the length of the spacer layer 32 is the same as the length of the first outer wall of the tie plate 3, and the width of the spacer layer 32 is smaller than the width of the tie plate 3.

Referring to fig. 6 to 7 and 9 to 10, in order to ensure light transmittance of the tie plate 3, the interlayer 32, the head fixing piece 33, the tail fixing piece 34 and the partition 212, the tie plate 3, the interlayer 32, the head fixing piece 33, the tail fixing piece 34 and the partition 212 are made of polypropylene, so that the weight can be reduced and the light receiving degree of the experimental mice can be ensured.

Referring to fig. 2, in the present embodiment, the light emitting element includes at least one LED light bar 4, where the LED light bar 4 is fixedly disposed on the top end surface of the chamber 12, the LED light bar 4 is electrically connected to the control module, and the LED light bar 4 illuminates an area in the chamber 12 to simulate an experimental environment during daytime.

When the LED light bar 4 is arranged as one, the LED light bar 4 is combined on the top end face of the cavity 12 to form a closed graph, which is preferably rectangular, and when the LED light bar is used, the bound experimental mice can be shone in all directions, so that the illumination intensity of the experimental mice bound in batches is the same; more preferably, the LED light strip 4 is disposed along the edge of the top end face of the chamber 12.

When the LED light bars 4 are provided in a plurality of strips, the method comprises: the first light bar, the second light bar, the third light bar and the fourth light bar; the first light bar is parallel to the third light bar, the second light bar is parallel to the fourth light bar, the first light bar is perpendicular to the third light bar, the first light bar, the second light bar, the third light bar and the fourth light bar are not connected with each other, and in addition, the first light bar, the second light bar, the third light bar and the fourth light bar are electrically connected with the control module, so that the first light bar, the second light bar, the third light bar and the fourth light bar can be lightened simultaneously and turned off simultaneously.

Referring to fig. 1-3, in order to maintain air circulation in the chamber 12 of the case 1 during the experiment, a through-hole communicating with the chamber 12 is provided on the top outer wall of the case 1 in this embodiment, and an exhaust fan 5 is provided in the through-hole; in addition, the exhaust fan 5 is electrically connected with the control module; more specifically, the exhaust fan 5 adopts a low-noise exhaust fan 5 and is fixedly arranged in the through opening; thereby ensuring the ventilation in the box body 1, ensuring the novel air in the chamber 12 and preventing the ammonia concentration from exceeding 20 ppm.

In addition, on the basis of arranging the exhaust fan 5 on the box body 1, external light is prevented from irradiating into the cavity 12 through a gap of the exhaust fan 5, so that the experimental mouse circadian rhythm disorder modeling fails, therefore, a light shielding plate 51 is arranged on the top end face of the cavity 12, wherein a connecting rod is arranged on the same outer wall of the light shielding plate 51, and the light shielding plate 51 is connected with the top end face of the cavity 12 through the connecting rod, so that a space exists between the light shielding plate 51 and the top end face of the cavity 12, and the situation that the light shielding plate 51 is blocked in the exhaust fan 5 is avoided; more preferably, the length and width of the light shielding plate 51 are greater than the length and width of the through-hole; when in use, the external light passing through the gap of the exhaust fan 5 always falls on the light shielding plate 51, so that the external light is prevented from damaging the night experimental environment simulated by the chamber 12.

Referring to fig. 1-3, in this embodiment, the control module controls the lighting time of the LED light bar 4 and the illumination time of the experimental mouse; its control module sets up in the top outer wall of box 1, and wherein, control module includes: an operation key 61, a display screen 62, a power module, and a control main board; the top end face of the chamber 12 is provided with a placement body 6, the top outer wall of the box body 1 and the placement body 6 are internally provided with placement grooves, the power module and the control main board are arranged in the placement grooves, a top plate is arranged to seal the placement grooves, and an operation key 61 and a display screen 62 are arranged on the top plate and are electrically connected with the control main board; when in use, the operating key 61 sets the lighting time and the illumination time of the LED light bar 4 and displays the lighting time and the illumination time on the display screen 62, and the power module supplies power to the LED light bar 4, the control main board, the operating key 61, the display screen 62 and the exhaust fan 5.

In addition, the power module includes: a storage battery and a charging interface; the storage battery is arranged in the placing groove, the charging interface is arranged on any side wall of the box body 1, and the charging interface is electrically connected with the storage battery.

The power module may further include: a battery groove and a cover plate are arranged on any side wall of the box body 1; the battery jar is used for placing the battery, and the apron is used for carrying out the closing cap with the battery jar, and the apron can dismantle in the battery jar.

The application method of the invention comprises the following steps:

when the experimental mouse is used, the box door is opened, part of the frame body is separated from the cavity completely, the constraint plate is taken down in the placement area, the mice used for experiments are placed in the grooves of the constraint plate, the head fixing plate and the tail fixing plate are inserted into the first clamping groove and the second clamping groove according to the body type of the experimental mouse, the front and rear movement range of the experimental mouse is limited to be minimum, the constraint plate loaded with the experimental mouse is placed in the placement area of the frame body, the frame body is placed in the box body, the box door is closed, and the lighting time and the illumination time of the LED lamp strip are set through the operation keys; simultaneously, the exhaust fan is always in a working state.

The above description of embodiments is only for the understanding of the present invention. It should be noted that it will be apparent to those skilled in the art that modifications can be made to the present invention without departing from the principles of the invention, and such modifications will fall within the scope of the claims.

Claims

1. A device for studying a depressive mouse rhythm disorder, comprising:

a light assembly for inducing circadian rhythm disorders in an experimental animal, the light assembly comprising: the light-emitting device comprises a light-emitting element and a control module for controlling the start and stop of the light-emitting element, wherein the light-emitting element is arranged in the cavity; the control module is arranged on one side wall of the box body and is electrically connected with the light-emitting element;

the restraint device includes: the frame body is arranged in the cavity and is used for binding experimental animals and is connected with the binding assembly of the frame body, the frame body is provided with an upper layer and a plurality of supporting rods connected with the upper layer, the upper layer is provided with at least one placement area, and the binding assembly is arranged in the placement area;

the restraint assembly includes: a binding plate, a head fixing piece and a tail fixing piece; the binding plate is provided with a first outer wall, a plurality of grooves are formed in the first outer wall in an arrangement mode, the interlayer is arranged to shield the grooves, the head fixing piece is inserted into one end of each groove, the tail fixing piece is inserted into the other end of each groove, and the head fixing piece and the tail fixing piece can be separated from the grooves;

after the binding plate is placed in the placement area, the central line of the groove is perpendicular to the upper layer, so that the experimental mouse is in an upright state in the experimental process, and the head of the experimental mouse faces the light-emitting element.

2. The device for studying circadian rhythm disorders in depressed mice according to claim 1, wherein said chamber has a top end face and a bottom end face, wherein said light emitting element is provided at said top end face, and said binding means is radially translated at said bottom end face.

3. The device for studying circadian disorders of depressed mice according to claim 2, wherein the bottom end face of the chamber is provided with a slide, which is perpendicular to the door after the door covers the opening of the chamber, and one end of the slide near the opening is provided with an entrance, and the restraint device slides into the slide from the entrance.

4. The device for studying circadian rhythm disorders in depressed mice according to claim 3, wherein one ends of the plurality of support rods are connected to a connection end surface, and the other ends of the plurality of support rods are disposed in the slide rail.

5. The device for studying circadian rhythm disorders in depressed mice according to claim 4, wherein when the number of said placement areas is plural, a partition is provided between each adjacent placement area, the partition being perpendicular to the upper layer, and the restraint assembly is provided between the adjacent partition.

6. The device for studying circadian rhythm disorders in depressed mice according to claim 4, wherein said binding members are separable from said placement region, and the number of said binding members is the same as the number of said placement regions.

7. The device for studying circadian rhythm disorder in depressed mice according to claim 2, wherein the light-emitting element comprises: the LED lamp strip is fixedly arranged on the top end face of the cavity, and one end of the LED lamp strip is electrically connected with the control module.

8. The device for studying circadian rhythm disorder in depressed mice according to claim 7, wherein said LED light bars are combined into a closed pattern on said top end face of said chamber;

9. The device for studying circadian rhythm disorders in depressed mice according to claim 1, wherein said case further comprises: the exhaust fan is characterized in that the box body is provided with a top outer wall, and a through hole penetrating through the box wall of the box body is formed in the top outer wall; the exhaust fan is fixedly arranged at the through hole and partially extends into the cavity.