CN115474556A - Experimental device and experimental method for rat and mouse depression model - Google Patents
Experimental device and experimental method for rat and mouse depression model Download PDFInfo
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- CN115474556A CN115474556A CN202211318395.0A CN202211318395A CN115474556A CN 115474556 A CN115474556 A CN 115474556A CN 202211318395 A CN202211318395 A CN 202211318395A CN 115474556 A CN115474556 A CN 115474556A
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- 238000002474 experimental method Methods 0.000 title claims abstract description 58
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 53
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- 238000002347 injection Methods 0.000 claims description 7
- 239000007924 injection Substances 0.000 claims description 7
- 230000001629 suppression Effects 0.000 claims description 7
- 241000699670 Mus sp. Species 0.000 claims description 6
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- 239000007788 liquid Substances 0.000 abstract description 4
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/03—Housing for domestic or laboratory animals
- A01K1/031—Cages for laboratory animals; Cages for measuring metabolism of animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K1/00—Housing animals; Equipment therefor
- A01K1/02—Pigsties; Dog-kennels; Rabbit-hutches or the like
- A01K1/035—Devices for use in keeping domestic animals, e.g. fittings in housings or dog beds
- A01K1/0356—Feeding or drinking devices associated with cages
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K15/00—Devices for taming animals, e.g. nose-rings or hobbles; Devices for overturning animals in general; Training or exercising equipment; Covering boxes
- A01K15/02—Training or exercising equipment, e.g. mazes or labyrinths for animals ; Electric shock devices ; Toys specially adapted for animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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Abstract
The invention discloses a rat and mouse depression model experimental device and an experimental method, wherein a digital controller is arranged to control the opening and closing of a control valve so as to realize a water jet stimulation experiment, a fasting control mechanism is arranged to control feeding so as to realize a fasting stimulation experiment, a water tank is matched with a bidirectional pump to pump liquid in the water tank into a rat moving cavity so as to realize a water jet stimulation experiment, and a bottom plate driving mechanism drives a moving bottom plate to vibrate or tilt so as to realize a vibration stimulation experiment or a tilt stimulation experiment.
Description
Technical Field
The invention relates to the technical field of animal science experiments, in particular to the technical field of experimental devices for rat and mouse depression models.
Background
The chronic unpredictable animal stress model (CUMS) is a commonly used depression molding method at present, and the molding method is relatively close to the pathogenesis process of depression, so the method is widely applied to the construction of depression models of rats and mice. During CUMS stress, the experimental rats need to be deprived of drinking water, deprived of food, reversed day and night, stimulated by noise, moistened overnight, stimulated by electric shock, restrained in limited space and stimulated by a low-frequency flashlight for various times, and all animals in each batch of experiment have the same stimulation intensity, batch and sequence until the experiment is finished. The existing molding device is simple in structure, and a two-factor mouse depression molding device is provided as in the utility model patent of Chinese utility model with the publication number of CN 214155797U.
The molding device disclosed in the prior art has the advantages that the structure is simple, automation control stimulation cannot be realized, manual operation of experimenters is often needed, time and labor are wasted in various stimulation operations and recording, the efficiency is low, experimental failure caused by inconsistent experimental conditions is easily caused, stimulation projects are few, and the experiment is not comprehensive. In addition, the experiment cage under different models can not be replaced, is compatible and practical, needs a whole set of configuration, and causes the equipment cost to be improved. And because CUMS involves more stress types and emphasizes random, CUMS operation flows and experimental devices in various laboratories are almost different, and the standardization of depression model construction and the accurate replication of depression models are seriously influenced. Therefore, the construction of standard procedures and experimental devices becomes a key issue for the success and later reproduction of depression models.
Disclosure of Invention
The invention aims to solve the problems in the prior art, and provides a rat and mouse depression model experiment device and an experiment method, which can improve the automation degree of rat and mouse depression modeling experiments and enable stimulation factors to be more diversified.
In order to achieve the purpose, the invention provides a rat and mouse depression model experimental device which comprises a rat cage body and a cage cover detachably arranged at the top of the rat cage body, wherein a rat moving cavity is arranged in the rat cage body, a moving bottom plate is arranged at the bottom in the rat moving cavity, a bottom plate driving mechanism used for driving the moving bottom plate to incline and oscillate is arranged on the rat cage body, a water feeding bottle and a water bottle fixing part used for fixing the water feeding bottle are arranged at the side edge of the rat cage body, a control valve is arranged on the water feeding bottle fixing part, the input end of the control valve is communicated with the output end of the water bottle, a water feeding pipe is arranged at the output end of the control valve, the output end of the water feeding pipe extends into the rat moving cavity, a water tank is further arranged at the side edge of the rat cage body, a water injection pipe is arranged between the water tank and the rat moving cavity, a bidirectional pump is arranged on the water injection pipe, a plurality of illuminating lamp strips are arranged at the top in the rat moving cavity, a digital controller connected with the illuminating lamp strips, the control valve and the bottom plate driving mechanism, a timing module is arranged in the digital controller, a feeding control mechanism connected with the rat feeding control box, and a food fasting feeding mechanism is arranged in the rat feeding control box.
Preferably, the mouse cage further comprises a light shield detachably connected with the mouse cage body, and the light shield is connected with the mouse cage body in a sleeved mode.
Preferably, the bottom plate driving mechanism comprises a hinged shaft, the middle part of the movable bottom plate is hinged with the mouse movable cavity through the hinged shaft, a bottom plate driving motor is arranged at any end part of the side wall of the mouse cage box body close to the movable bottom plate, a rotating shaft of the bottom plate driving motor penetrates through the side wall of the mouse cage box body to extend into the mouse movable cavity and is provided with a driving arm, a connecting rod hinged with the end part of the driving arm is arranged at the end part of the driving arm, and the other end of the connecting rod is hinged with the end part of the movable bottom plate.
Preferably, a monitoring camera and a loudspeaker electrically connected with the digital controller are further arranged in the mouse activity cavity.
Preferably, the fasting control mechanism comprises a door plate corresponding to the feeding port, a door opening and closing motor for driving the door plate to rotate is arranged on the squirrel-cage box body, the door opening and closing motor is fixedly connected with one side of the door plate, and open grooves for the door plate to pass through are formed in the upper end and any side face of the squirrel-cage box body.
Preferably, the feed box and the food supplementing opening are both arranged on the upper side of the movable bottom plate.
Preferably, the mouse activity intracavity still be equipped with and hang tail mechanism, hang tail mechanism including transversely locating the inner plate body in the mouse activity intracavity, be equipped with a plurality of electromagnetic plates on the inner plate body, mouse activity intracavity still is equipped with the mouse tail buckle that is used for being fixed in on the mouse tail, the mouse tail buckle with electromagnetic plate magnetism is inhaled the cooperation, digital controller with electromagnetic plate control connection.
Preferably, the tail suspension mechanism further comprises a plate lifting mechanism used for driving the inner plate body to lift up and down, the plate lifting mechanism comprises an electric push rod vertically arranged on the cage cover, a lever arm of the electric push rod penetrates through the cage cover and then is fixedly connected with the inner plate body, and the digital controller is in control connection with the electric push rod.
Preferably, the mouse tail retaining ring comprises a first semi-ring and a second semi-ring, one end of the first semi-ring is hinged to the second semi-ring, the other end of the first semi-ring is connected with the second semi-ring in a clamping mode, and anti-skidding inner pads for being attached to a mouse tail are arranged on inner rings of the first semi-ring and the second semi-ring.
Preferably, the inner plate body is a transparent or semitransparent light guide plate, and the lighting lamp strip is fixedly arranged on the upper side of the inner plate body and is arranged at an interval with the electromagnetic plate.
Preferably, the mouse tail buckle is characterized by further comprising a body sleeve for fixing the body of a rat or a mouse, wherein the body sleeve comprises a lace and a sleeve shell fixedly arranged on the lace, two ends of the lace are detachably connected through a buckle, and a steel wire rope connected with the mouse tail buckle ring is arranged on the sleeve shell.
Preferably, a storage battery and a control circuit board are arranged in the casing, at least two electric shock contacts arranged towards the mouse body are arranged on the inner side of the casing, and the control circuit board is electrically connected with the electric shock contacts.
Preferably, a first wireless communication module is arranged in the control circuit board, a second communication module in communication connection with the first wireless communication module is arranged in the digital controller, and a WIFI module or a 4G/5G communication module is further arranged in the digital controller.
The invention also aims to provide a rat and mouse depression model experiment method which comprises the rat and mouse depression suppression model experiment device, wherein the rat and mouse depression suppression model experiment device is placed in a quiet room, a rat and a mouse are placed in the rat moving cavity, and the rat and the mouse are subjected to stimulation experiments by controlling a proper stimulation mode through a digital controller according to experiment requirements.
The experimental device and the experimental method for the rat and mouse depression model have the beneficial effects that: the experimental device can simulate various stimulation modes and simulate various depression symptom modeling processes, the experimental process can be controlled automatically through the digital controller, experimenters are liberated, the experimenters are also reduced from interference on the experimenters, and the interference of the external environment on the accuracy of experimental results is reduced, so that the standardization and the repeatability of depression modeling processes can be ensured, the tail suspension mechanism is arranged, the tail suspension stimulation experiment can be automatically carried out on big and small mice, the tail suspension stimulation experiment can be started and stopped at any time, the experimental process is automatically controlled by the digital controller, the automation degree is higher, and the remote control is more convenient.
The features and advantages of the present invention will be described in detail by embodiments in conjunction with the accompanying drawings.
Drawings
Fig. 1 is a schematic structural diagram of a main view of a rat and mouse depression model experimental device.
Fig. 2 is a schematic structural diagram of a main view section of the experimental device for the rat and mouse depression model.
Fig. 3 is a schematic top view structure diagram of a rat and mouse depression model experimental device of the present invention.
Fig. 4 is a schematic rear view structure diagram of the experimental device for the rat and mouse depression model.
Fig. 5 is a schematic top view of a fasting controlling mechanism of the experimental device for the rat and mouse depression model of the present invention.
Fig. 6 is a schematic view of a rat tail clasp structure of the experimental device for rat and mouse depression models.
Fig. 7 is a schematic structural diagram of a body sleeve of the experimental device for the rat and mouse depression model.
Fig. 8 is a schematic structural diagram of a light shield of the rat and mouse depression model experimental device.
Fig. 9 is a schematic view of a combination structure of a light shield and a squirrel cage box of the rat and mouse depression model experimental device.
Wherein:
1-covering a cage; 2-a lighting strip; 3-squirrel cage box body; 4-a movable bottom plate; 5-a digital controller; 7-water feeding bottle; 7-water feeding bottle; 8-a water injection pipe; 9-a water tank; 11-a cage cover; 12-an inner plate body; 13-an electromagnetic plate; 14-a rat tail clasp ring; 15-electric push rod; 16-a body cover; 17-a hinged axis; 18-a backplane drive motor; 19-a surveillance camera; 20-a loudspeaker; 21-a feed box; 22-door panel; 23-a door opening and closing motor; 24-a light shield; 31-rat active cavity; 32-nourishing mouth; 71-water bottle holder; 72-a control valve; 73-water supply pipe; 81-a bidirectional pump; 141-a first half-ring; 142-a second half ring; 143-anti-slip inner pad; 161-tie; 162-a casing; 163-fastener; 164-a steel cord; 166-shock contacts; 181-a drive arm; 182-a link.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to the accompanying drawings and examples. It should be understood, however, that the description herein of specific embodiments is only intended to illustrate the invention and not to limit the scope of the invention. Moreover, in the following description, descriptions of well-known structures and techniques are omitted so as to not unnecessarily obscure the concepts of the present invention.
In the description of the present invention, it should be noted that when an element is referred to as being "fixed" or "disposed" to another element, it can be directly on the other element or be indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or be indirectly connected to the other element.
In the description of the present invention, it should be noted that the terms "center", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings or the orientations or positional relationships in which the products of the present invention are conventionally placed when used, and are merely used for convenience of describing and simplifying the description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first," "second," "third," and the like are used solely to distinguish one from another, and are not to be construed as indicating or implying relative importance. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically defined otherwise. The meaning of "a number" is one or more unless specifically limited otherwise.
In the description of the present invention, it should also be noted that, unless otherwise explicitly stated or limited, the terms "disposed," "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in a specific case to those of ordinary skill in the art.
The first embodiment is as follows:
referring to fig. 1, 2, 3 and 4, the rat depression model experimental device comprises a rat cage body 3 and a cage cover 1 detachably arranged at the top of the rat cage body 3, a rat moving cavity 31 is arranged in the rat cage body 3, a moving bottom plate 4 is arranged at the bottom in the rat moving cavity 31, a bottom plate driving mechanism for driving the moving bottom plate 4 to incline and oscillate is arranged on the rat cage body 3, a water feeding bottle 7 and a water bottle fixing member 71 for fixing the water feeding bottle 7 are arranged at the side edge of the rat cage body 3, a control valve 72 is arranged on the water feeding bottle fixing member 71, the input end of the control valve 72 is communicated with the output end of the water bottle 7, a water feeding pipe 73 is arranged at the output end of the control valve 72, the output end of the water feeding pipe 73 extends into the rat moving cavity 31, a water tank 9 is further arranged at the side edge of the rat cage body 3, a water injection pipe 8 is arranged between the water tank 9 and the rat moving cavity 31, a bidirectional pump 81 is arranged on the water injection pipe 8, a plurality of lighting lamp strips 2 are arranged at the top in the rat moving cavity 31, a digital control module for driving the rat moving control valve 5 is connected with the digital control module 5 and the digital control module 81 and the mouse moving control module 5. Thereby this embodiment accessible sets up the switching of digital controller 5 control valve 72 and realizes forbidding water thorn and swashs the experiment, thereby through setting up the control mechanism that fasts and feed and eat and realize the amazing experiment of fasting, through water tank 9 and the cooperation of bi-directional pump 81, take out liquid in the water tank 9 in the mouse activity chamber 31, thereby realize the water logging amazing experiment, drive movable bottom plate 4 vibration or slope through bottom plate drive mechanism, thereby realize the amazing experiment of vibration or the amazing experiment of slope, this application experimental apparatus can simulate multiple amazing mode, simulate multiple depression symptom modeling process, 5 full automation control of experiment process accessible digital controller, liberation experimenter, also reduce the interference of experimenter to the laboratory mouse, reduce the interference of external environment to the experimental result accuracy, thereby can guarantee the standardization and the repeatability of depression modeling process, thereby guarantee the accuracy of experiment.
Example two:
referring to fig. 8 and 9, the mouse cage further comprises a light shield 24 detachably connected with the mouse cage body 3, and the light shield 24 is connected with the mouse cage body 3 in a sleeved mode. The lens hood 24 can block external light from entering the squirrel cage box body 3, so that the circadian rhythm is adjusted by adjusting the internal brightness of the illuminating lamp strip 2, and the laboratory mouse is stimulated.
Referring to fig. 2, a monitoring camera 19 and a speaker 20 electrically connected to the digital controller 5 are further disposed in the mouse activity chamber 31. The monitoring camera 19 can be used for monitoring and recording the condition of the laboratory mouse in real time, so that experimenters can observe in real time conveniently, and the loudspeaker 20 can be used for making sound to stimulate the laboratory mouse, so as to achieve the purpose of sound stimulation.
Referring to fig. 2, 3 and 5, the fasting control mechanism includes a door plate 22 corresponding to the feeding port 32, a door opening and closing motor 23 for driving the door plate 22 to rotate is disposed on the cage body 3, the door opening and closing motor 23 is fixedly connected to one side of the door plate 22, and open slots for the door plate 22 to pass through are disposed at the upper end and any side of the cage body 3. The opening and closing of the food supplementing opening 32 can be controlled by controlling the door plate 22 to rotate through the door opening and closing motor 23, the control is more convenient, and when the food supplementing opening 32 needs to be opened, the door opening and closing motor 23 controls the door plate 22 to rotate to the position of the food supplementing opening 32 to shield the food supplementing opening 32; when the food supplementing opening 32 needs to be opened, the door opening and closing motor 23 controls the door plate 22 to rotate reversely, the door plate 22 rotates to the outside of the squirrel cage box body 3 through the open slot, the food supplementing opening 32 can be opened, and the control is more convenient.
Referring to fig. 2, the feed box 21 and the feeding hole 32 are both disposed on the upper side of the movable bottom plate 4. The control valve 72 is prevented from wetting the feed in the feed box 21 when the water in the water tank 9 is introduced into the rat moving cavity 31.
Example three:
referring to fig. 2 and 6, a tail suspension mechanism is further arranged in the mouse moving cavity 31, the tail suspension mechanism comprises an inner plate body 12 transversely arranged in the mouse moving cavity 31, a plurality of electromagnetic plates 13 are arranged on the inner plate body 12, a mouse tail retaining ring 14 used for being fixed on a mouse tail is further arranged in the mouse moving cavity 31, the mouse tail retaining ring 14 is in magnetic attraction fit with the electromagnetic plates 13, and the digital controller 5 is in control connection with the electromagnetic plates 13. Still establish in addition in this embodiment and hang tail mechanism, can hang tail stimulation experiment to big or small mouse, further improve the stimulation means of this application, this application hangs tail mechanism and constitutes through tail buckle 14 and electromagnetic plate 13, and control through digital controller 5, when hanging tail stimulation, can be directly adsorb the electromagnetic plate 13 position of inner plate body 12 through tail buckle 14, thereby hang tail stimulation, digital controller 5 accessible is to the demagnetization of 13 outage of electromagnetic plate after the certain period, thereby terminate and hang the tail experiment, it is more convenient to control, the test duration is more convenient to control.
Referring to fig. 2 and 6, the tail suspension mechanism further comprises a board lifting mechanism for driving the inner board body 12 to lift up and down, the board lifting mechanism comprises an electric push rod 15 vertically arranged on the cage cover 1, a lever arm of the electric push rod 15 penetrates through the cage cover 1 and then is fixedly connected with the inner board body 12, and the digital controller 5 is in control connection with the electric push rod 15. Elevating system can drive the height-adjusting of inner plate body 12, when needing to hang the tail experiment, elevating system can drive inner plate body 12 and descend, make inner plate body 12 be close to mouse tail buckle 14, be convenient for electromagnetic plate 13 and mouse tail buckle 14 adsorb, just can control inner plate body 12 after adsorbing and rise and hang the tail experiment, electromagnetic plate 13 can cut off the power supply again and demagnetize the termination and hang the tail after the certain time, this embodiment hangs tail mechanism and can realize hanging the tail experiment automatically, need not the manual control, the experimentation is more convenient, liberation experimenter.
Referring to fig. 2 and 6, the mouse tail retaining ring 14 includes a first half ring 141 and a second half ring 142, one end of the first half ring 141 is hinged to the second half ring 142, the other end of the first half ring 141 is connected to the second half ring 142 in a snap-fit manner, and inner rings of the first half ring 141 and the second half ring 142 are provided with anti-slip inner pads 143 for being attached to a mouse tail. First half-ring 141 and second half-ring 142 cooperate each other to be convenient for block on the tail, and the installation is dismantled conveniently, and the antiskid inner pad 143 can improve frictional force.
Referring to fig. 2, the inner plate 12 is a translucent light guide plate, and the lighting strip 2 is fixedly disposed on the upper side of the inner plate 12 and spaced from the electromagnetic plate 13.
Example four:
referring to fig. 2 and 7, the body cover 16 for fixing the trunk of a rat or mouse is further included, the body cover 16 includes a lace 161 and a sleeve 162 fixed on the lace 161, two ends of the lace 161 are detachably connected through a buckle 163, and a steel cable 164 connected with the tail buckle 14 is disposed on the sleeve 162. The body cover 16 is used for being fixed at the waist and abdomen position of the laboratory mouse, the position is fixed stably, and the tail retaining ring 14 is connected through the steel wire rope 164, so that the fixing firmness degree of the tail retaining ring 14 is improved, and the tail is prevented from being separated from the rat.
Referring to fig. 2 and 7, a storage battery and a control circuit board are disposed in the casing 162, two electric shock contacts 166 facing the mouse body are disposed on the inner side of the casing 162, and the control circuit board is electrically connected to the electric shock contacts 166. The electric shock stimulation can be carried out on the rats and mice through the electric shock contact 166, and the experimental range which can be carried out by the device is further increased.
Referring to fig. 2 and 7, a first wireless communication module is disposed in the control circuit board, a second communication module communicatively connected to the first wireless communication module is disposed in the digital controller 5, and a 4G/5G communication module is further disposed in the digital controller 5. Digital controller 5 is convenient for carry out the electric shock through first wireless communication module and second communication module's cooperation wireless control circuit board amazing, and it is more convenient to control, need not the wire and connects, avoids influencing the laboratory mouse activity, sets up 4G 5G communication module and is convenient for the experimenter remote control digital controller 5.
Example five:
the embodiment provides a rat and mouse depression model experiment method, which comprises the rat and mouse depression suppression model experiment device as claimed in any one of claims 1 to 9, placing the rat and mouse depression suppression model experiment device in a quiet room, placing a rat and a mouse in the rat activity cavity 31, and performing stimulation experiment on the rat and the mouse by controlling a proper stimulation mode through the digital controller 5 according to experiment requirements.
Preferably, the stimulation experiment comprises the steps that the digital controller 5 controls the control valve 72 to be opened and closed so as to realize the water-forbidden stimulation experiment; the fasting control mechanism controls feeding so as to realize a fasting stimulation experiment; the water tank 9 is matched with the bidirectional pump 81, so that liquid in the water tank 9 is pumped into the mouse moving cavity 31, and a water immersion stimulation experiment is realized; the movable bottom plate 4 is driven by the bottom plate driving mechanism to vibrate or incline, so that the vibration stimulation experiment or the inclined stimulation experiment is realized.
Preferably, the stimulation experiment further comprises: the lifting mechanism of the lifting mechanism can be controlled by the digital controller 5 to drive the inner plate body 12 to descend, so that the inner plate body 12 is close to the mouse tail retaining ring 14, the electromagnetic plate 13 is electrified and magnetically conductive, the electromagnetic plate 13 is adsorbed by the mouse tail retaining ring 14, the inner plate body 12 is controlled to rise after adsorption to carry out tail suspension experiment, and the electromagnetic plate 13 is de-electrified and degaussed after a certain time to terminate tail suspension experiment.
Preferably, the stimulation experiment further comprises: the electric shock contacts 166 are controlled by the digital controller 5 to perform electric shock stimulation on the rats and mice.
The working process of the invention is as follows:
in the working process of the rat and mouse depression model experimental device, the digital controller 5 controls the control valve 72 to open and close so as to realize a water-jet inhibition experiment, the fasting control mechanism is arranged to control feeding so as to realize a fasting stimulation experiment, the water tank 9 is matched with the bidirectional pump 81 to pump liquid in the water tank 9 into the rat movable cavity 31 so as to realize the water-jet stimulation experiment, and the bottom plate driving mechanism drives the movable bottom plate 4 to vibrate or incline so as to realize an oscillation stimulation experiment or an inclined stimulation experiment.
Standard parts used in the application document can be purchased from the market, the specific connection mode of each part adopts conventional means such as mature bolts, rivets, welding and the like in the prior art, the internal parts of the electric sliding rail sliding seat, the air cylinder, the welding machine, the electric telescopic rod and the controller all adopt conventional models in the prior art, the internal structure of the electric sliding rail sliding seat, the air cylinder, the welding machine, the electric telescopic rod and the controller belongs to the structure of the prior art, a worker can complete normal operation of the electric sliding rail sliding seat according to a manual of the prior art, the circuit connection adopts the conventional connection mode in the prior art, and specific description is not made.
It should be noted that, although the above embodiments have been described herein, the scope of the present invention is not limited thereby. Therefore, based on the innovative concepts of the present invention, the technical solutions of the present invention can be directly or indirectly applied to other related technical fields by making changes and modifications to the embodiments described herein, or by using equivalent structures or equivalent processes performed in the content of the present specification and the attached drawings, which are included in the scope of the present patent.
Claims (10)
1. The utility model provides a big mouse depression model experimental apparatus, includes squirrel cage box (3) and can dismantle and locate cage lid (1) at squirrel cage box (3) top, be equipped with mouse activity chamber (31) in squirrel cage box (3), the bottom is equipped with movable bottom plate (4), its characterized in that in mouse activity chamber (31): the mouse cage is characterized in that a bottom plate driving mechanism used for driving the movable bottom plate (4) to incline and oscillate is arranged on the mouse cage body (3), a water feeding bottle (7) and a water bottle fixing piece (71) used for fixing the water feeding bottle (7) are arranged on the side of the mouse cage body (3), a control valve (72) is arranged on the water feeding bottle fixing piece (71), the input end of the control valve (72) is communicated with the output end of the water bottle (7), a water feeding pipe (73) is arranged at the output end of the control valve (72), the output end of the water feeding pipe (73) extends into the mouse movable cavity (31), a water tank (9) is further arranged on the side of the mouse cage body (3), a water injection pipe (8) is arranged between the water tank (9) and the mouse movable cavity (31), a bidirectional pump (81) is arranged on the water injection pipe (8), a plurality of illuminating lamp strips (2) are arranged at the top in the mouse movable cavity (31), a digital controller (5) controlled and connected with the bottom plate driving mechanism is arranged on the mouse cage body (3), a feed module (21) is arranged outside the mouse cage body, and a feed module (21) is arranged on the side of the mouse cage body, the mouse cage is characterized in that a food supplementing opening (32) communicated with the mouse moving cavity (31) and the feed containing cavity is formed in the side wall of the mouse cage body (3), a fasting control mechanism used for controlling the food supplementing opening (32) to be opened and closed is arranged on the mouse cage body (3), and the digital controller (5) is in control connection with the fasting control mechanism.
2. The experimental device for rat and mouse depression model as claimed in claim 1, wherein: the mouse cage is characterized by further comprising a light shield (24) detachably connected with the mouse cage body (3), and the light shield (24) is connected with the mouse cage body (3) in a sleeved mode.
3. The experimental device for the depression model of mice and rats as set forth in claim 1, wherein: a monitoring camera (19) and a loudspeaker (20) which are electrically connected with the digital controller (5) are also arranged in the mouse activity cavity (31).
4. The experimental device for rat and mouse depression model as claimed in claim 1, wherein: the food fasting control mechanism comprises a door plate (22) corresponding to the food supplementing opening (32), a door opening and closing motor (23) used for driving the door plate (22) to rotate is arranged on the squirrel-cage box body (3), the door opening and closing motor (23) is fixedly connected with one side of the door plate (22), and open grooves for the door plate (22) to pass through are formed in the upper end and any side face of the squirrel-cage box body (3).
5. The experimental device for rat and mouse depression model as claimed in claim 4, wherein: the feed box (21) and the food supplementing opening (32) are arranged on the upper side of the movable bottom plate (4).
6. The experimental device for rat and mouse depression model as claimed in claim 1, wherein: still be equipped with in mouse activity chamber (31) and hang tail mechanism, hang tail mechanism including transversely locating interior plate body (12) in mouse activity chamber (31), be equipped with a plurality of electromagnetic plates (13) on interior plate body (12), still be equipped with in mouse activity chamber (31) and be used for being fixed in the mouse tail buckle (14) on the mouse tail, mouse tail buckle (14) with cooperation is inhaled to electromagnetic plate (13) magnetism, digital controller (5) with electromagnetic plate (13) control connection.
7. The experimental device for rat and mouse depression model as claimed in claim 6, wherein: hang tail mechanism still including being used for the drive the board elevating system of interior plate body (12) oscilaltion, board elevating system includes vertical locating electric putter (15) on cage lid (1), electric putter (15) lever arm runs through behind cage lid (1) with interior plate body (12) fixed connection, digital controller (5) with electric putter (15) control connection.
8. The experimental device for the depression model of mice and rats as set forth in claim 6, wherein: the mouse tail retaining ring (14) comprises a first half ring (141) and a second half ring (142), one end of the first half ring (141) is hinged to the second half ring (142), the other end of the first half ring is connected with the second half ring (142) in a clamping mode, and anti-skidding inner pads (143) used for being attached to a mouse tail are arranged on inner rings of the first half ring (141) and the second half ring (142).
9. The experimental device for rat and mouse depression model as claimed in claim 6, wherein: the inner plate body (12) is a transparent or semitransparent light guide plate, and the lighting lamp strip (2) is fixedly arranged on the upper side of the inner plate body (12) and is arranged at an interval with the electromagnetic plate (13).
10. An experimental method for a rat and mouse depression model is characterized in that: the rat and mouse depression suppression model experimental device comprises the rat and mouse depression suppression model experimental device as defined in any one of claims 1-9, wherein the rat and mouse depression suppression model experimental device is placed in a quiet room, a rat and a mouse are placed in the rat moving cavity (31), and the rat and the mouse are subjected to stimulation experiments by controlling a proper stimulation mode through the digital controller (5) according to experimental requirements.
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| CN202211318395.0A CN115474556B (en) | 2022-10-26 | 2022-10-26 | Experimental device and experimental method for rat depression model |
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| CN202211318395.0A CN115474556B (en) | 2022-10-26 | 2022-10-26 | Experimental device and experimental method for rat depression model |
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