CN115918559A - Device for establishing mouse chronic constraint stress model - Google Patents
Device for establishing mouse chronic constraint stress model Download PDFInfo
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- CN115918559A CN115918559A CN202310213866.XA CN202310213866A CN115918559A CN 115918559 A CN115918559 A CN 115918559A CN 202310213866 A CN202310213866 A CN 202310213866A CN 115918559 A CN115918559 A CN 115918559A
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Abstract
The invention belongs to the technical field of medical instruments, and particularly relates to a device for establishing a mouse chronic restraint stress model, which is mainly used for: constructing a depression experimental mouse model accompanied with circadian rhythm disorder, wherein an experimental environment box body for constructing a mouse, a frame body which is arranged in a cavity and used for bearing the constrained experimental mouse, and constraint components which are arranged in the placement area and used for constraining the experimental mouse are arranged, wherein the number of the constraint components is at least one; mainly solves the problems that: when a depression experimental mouse model accompanied with circadian rhythm disorder is constructed, a large quantity of experimental mice need to be bound, and instruments used in the existing experiment can only be bound to a single experimental mouse, so that the experimental mouse needs to be used in batches during the experiment, and a large amount of time of a researcher needs to be consumed to fix the mouse, so that the experimental period is prolonged.
Description
Technical Field
The invention belongs to the technical field of medical instruments, and particularly relates to a device for establishing a mouse chronic restraint stress model.
Background
Depression is a global mental disease characterized by low mood, slow thinking, hypovolemia, cognitive impairment, etc. When the depression is repeatedly developed and reaches moderate or severe degree, it may have serious influence on the work and life of the patient.
In clinical studies, it is an important experimental paradigm for studying mental diseases represented by depression to construct a chronic restraint stress model by using experimental mice, and in the current studies, the design similar to a centrifuge tube is often adopted for chronic restraint of mice, the mice are filled into the tube, and holes are punched on the outer wall of the restraint tube of the mice (for example, chinese patent: CN 203861377U).
The invention aims at the problems and provides a device for establishing a mouse chronic restraint stress model.
Disclosure of Invention
In order to overcome the problems in the background art, the invention adopts the following technical scheme:
a mouse chronic restraint stress model building device comprises:
the mouse experiment device comprises a box body and a control module, wherein the box body is used for constructing an experiment environment of a mouse, the box body is provided with a chamber which is used for placing an experimental animal and is provided with an opening, and a box door which can completely cover the opening of the chamber, and the box door is movably connected to the box body or detachably connected to the box body;
the rack body is configured in the cavity and used for bearing the restrained experimental mouse, and the rack body is provided with at least one placing area;
the binding assembly is arranged in the placing area and used for binding the experimental mice, the number of the binding assemblies is at least one, the binding assembly is provided with a plurality of binding spaces for binding the experimental mice, each binding space can be relatively reduced or increased, and each binding space can be locked after being reduced or increased.
In some embodiments of the present application, the magazine includes: the supporting device comprises a placing layer and a supporting rod, wherein the placing layer is provided with a first end face and a second end face, the placing area is arranged on the first end face, and the supporting rod is arranged on the second end face.
Furthermore, blocking pieces are arranged between two opposite edges of the first end face of the placing layer and the adjacent placing area, wherein the blocking pieces are parallel, and the blocking pieces are perpendicular to the first end face of the placing layer.
In some embodiments of the present application, the number of tie down assemblies and placement areas is the same.
In some embodiments of the present application, the tie-down assembly comprises: the binding plate is provided with a first layer and a second layer, a first side wall and a second side wall are arranged to connect the first layer with the second layer, and the first layer is provided with a sliding groove;
the locking assemblies are partially arranged in the sliding groove and move in the sliding groove in the radial direction;
the plurality of partition plates are arranged between the first layer and the second layer, each partition plate is connected with one locking assembly, and the bound spaces are arranged between the adjacent partition plates, between the first side wall and the partition plate closest to the first side wall and between the second side wall and the partition plate closest to the second side wall; when the locking assembly moves in the chute, the partition plate moves synchronously with the locking assembly; so that the bound space is increased or decreased.
The fixing pieces are inserted into the bound space, and the width of the fixing pieces is changed in a self-adaptive mode along with the increase or decrease of the bound space.
Further, the number of the locking assemblies is the same as that of the partition plates.
Further, the chute includes: the first sliding chute, the second sliding chute and the third sliding chute; the first sliding groove is arranged between the second sliding groove and the third sliding groove, and the locking component is partially configured on the first sliding groove, the second sliding groove and the third sliding groove and moves in the first sliding groove, the second sliding groove and the third sliding groove.
Optionally, the center line of the first sliding groove is the same as the center line of the second sliding groove and the center line of the third sliding groove, and the center line of the first sliding groove is parallel to the center lines of the second sliding groove and the third sliding groove.
In some embodiments of the present application, a plurality of the fixing members each include: the first part and the second part, the second part disposes with the first part in, and the second part can partly lean out the first part, makes the width of the first part and the width that the second part leaned out sum the width that always is the same with the width of bounding the space.
In some embodiments of the present application, a plurality of the locking assemblies each comprise: the sliding part is partially arranged in the second sliding groove and the third sliding groove and is connected with the partition board, and the sliding part is also provided with an accommodating cavity;
a locking member; the first sliding chute is arranged in the accommodating cavity and the first sliding chute and has a locking state and a releasing state when in use;
a button; part of the locking piece is arranged in the accommodating cavity and acts on the locking piece, and the locking piece can be driven to be switched between the locking state and the releasing state;
a seesaw; the button is pivotally arranged in the accommodating cavity and used for connecting the button and the locking piece, wherein one end of the rocker is connected with the locking piece, the other end of the rocker is connected with the button, and the button drives the locking piece through the rocker when the button moves;
a second spring; one end of the second spring is connected with the locking piece, and the other end of the second spring is connected with the button, so that the locking piece is always kept in the locking state.
Further, the locking piece is provided with a first contact wall and a second contact wall, wherein when the locking piece is in the locking state, the first contact wall and the second contact wall are always pressed on the first layer of the binding plate under the action of a second spring; the retaining member has a gap between the first contact wall, the second contact wall and the first layer of the tie plate in the released state.
The invention has the beneficial effects that: the experiment mice are bound in batches by arranging the binding component, so that the time for fixing the experiment mice is shortened;
through set up the changeable constraint space in space on the constraint subassembly, realized can carrying out the reduction or the increase in constraint space according to experimental mice's size for experimental mice is in reasonable experimental environment all the time.
Drawings
FIG. 1 is a schematic view of the structure of the bracket of the present invention in a box;
FIG. 2 is a schematic view of a chamber structure according to the present invention;
FIG. 3 is a schematic structural diagram of the case of the present invention;
FIG. 4 is a schematic view of a stent structure according to the present invention;
FIG. 5 is a schematic view of the bottom end face structure of the bracket of the present invention;
FIG. 6 is a schematic view of the mechanism on the tie down assembly and bracket of the present invention;
FIG. 7 is a schematic view of a structure of a binding plate according to the present invention;
FIG. 8 is a schematic view of the fastener of the present invention with increased width;
FIG. 9 is a schematic view of the fastener of the present invention with a reduced width;
FIG. 10 is a schematic view of the locking assembly of the present invention;
FIG. 11 is a schematic view of a slider according to the present invention;
FIG. 12 is a schematic view showing a locking state of the locker of the present invention;
FIG. 13 is a schematic view showing a release state of the locker of the present invention;
in the figure, 1, a box body; 11. a box door; 12. a chamber; 13. a strip of light; 14. an operation key; 15. a display screen; 16. an exhaust fan; 17. a visor; 2. a frame body; 21. placing the layer; 22. a support bar; 23. a stainless steel strip; 24. a placement area; 25. a barrier; 3. a binding plate; 31. a first layer; 311. a first chute; 312. a second chute; 313. a third chute; 32 a second layer; 33. a locking assembly; 34. a partition plate; 341. a bounding space; 342. a first card slot; 343. a second card slot; 35. a head fixing member; 36. a tail fixture; 4. a slider; 41. a locking member; 411. a first contact wall; 412. a second contact wall; 42. a button; 43. a first rocker; 431. a first end; 432. a second end; 433. a first pivot portion; 44. a second rocker; 441. a third end; 442. a fourth end; 443. a second pivot portion; 45. a second spring.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below by specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and other advantages and effects of the present invention can be easily understood by those skilled in the art from the disclosure of the present specification. The present invention can be implemented or applied by other different specific embodiments, and the features in the following embodiments and embodiments can be combined with each other without conflict, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
FIGS. 1 to 4 and 6 show the main technical contents of the present embodiment, and the present embodiment provides a mouse chronic restraint stress model building apparatus, which comprises
The box 1 is used for constructing an experimental environment of a mouse, the box 1 is provided with a chamber 12 used for placing an experimental animal and having an opening, and a box door 11 capable of completely covering the opening of the chamber 12, wherein the box door 11 is movably connected to the box 1 or the box door 11 is detachably connected to the box 1.
The rack 2 is disposed in the chamber 12 and used for carrying the restrained laboratory mouse, wherein the rack 2 has at least one placement area 24.
And a binding assembly disposed in the placement region 24 for binding the laboratory mouse, wherein the number of the binding assemblies is at least one, the binding assembly has a plurality of binding spaces 341 for binding the laboratory mouse, each binding space 341 can be relatively reduced or increased, and each binding space 341 can be locked after being reduced or increased.
The application is mainly used for constructing a depression experimental mouse model accompanied with circadian rhythm disorder.
Referring to fig. 1-3, in the present embodiment, in order to induce circadian rhythm disorder of a mouse after chronic confinement, in the present embodiment, a light bar 13 is disposed in a cavity 12, and a control module coupled to the light bar 13 is disposed on a side wall of a box body 1, specifically, the box body 1 has a first outer wall, a second outer wall, and a top outer wall; the control module is arranged on the side wall of the top of the box body 1 and used for controlling the lighting time of the light bar 13 and the illumination time of the experimental mouse; specifically, the control module includes: the light bar illuminating device comprises an operating key 14, a display screen 15, a power supply module and a control main board, wherein the operating key 14 and the display screen 15 are arranged on the outer wall of the top of the box body 1, the operating key 14 is used for controlling starting and stopping of the light bar 13 and inputting the lighting time and the lighting duration of the light bar 13, and the display screen 15 is used for displaying information input by the operating key 14; the power module and the control main board are arranged in the outer wall of the top of the box body 1, when the power module and the control main board are used, the lighting time and the illumination time of the light bar 13 are set through the operation key 14, the light bar and the display screen 15 are displayed, and the power module supplies power for the light bar 13, the control main board, the operation key 14 and the display screen 15.
In addition, the power supply module includes: a storage battery and a charging interface; wherein, set up the arrangement piece with in the cavity 12 to set up the standing groove with the top outer wall of arrangement piece and box 1, the battery sets up in the standing groove, and the interface that charges sets up in the top outer wall of box 1, and the interface that charges is in battery electric connection.
The power module may further include: a battery jar and a cover plate are arranged on the outer wall of the top of the box body 1; the battery jar is used for placing a battery, the cover plate is used for sealing the battery jar, and the cover plate can be detached from the battery jar.
Referring to fig. 1-2, in this embodiment, when the box door 11 is movably connected to the box body 1, the box body 1 and the box door 11 are connected by a hinge, wherein the hinge includes: the hinge comprises a first hinge, a second hinge and a pivot; the first hinge is fixed to a first outer wall of the box body 1 through a screw, the second hinge is fixed to an outer wall of the box door 11 through a screw, the first hinge and the second hinge are connected through a pivot, and the first hinge and the second hinge rotate with a circle center of the pivot as a base point, so that the box door 11 can completely cover an opening of the chamber 12.
In addition, in order to ensure that the door 11 cannot be opened when covering the opening of the chamber 12, in this embodiment, first magnetic stripes are arranged along at least two edges of the opening, a second magnetic stripe is arranged on the outer wall of the door 11 connected with the second hinge, and when the first magnetic stripe and the second magnetic stripe are close to each other, the first magnetic stripe and the second magnetic stripe attract each other, so that the door 11 always covers the opening of the chamber 12; so that the chamber 12 of the box body 1 is always kept in a relatively closed and dark experimental environment; the use of light assemblies to control the lighting environment within the chamber 12 is facilitated.
In order to keep the air in the chamber 12 circulating during the experiment, referring to fig. 1, in the present embodiment, a vent communicated with the chamber 12 is disposed on the second sidewall of the box 1, and an exhaust fan 16 is disposed at the vent, wherein the exhaust fan 16 is electrically connected to the control module, and more specifically, the exhaust fan 16 is a low-noise exhaust fan 16 and is fixedly disposed in the through hole; therefore, the air circulation in the box body 1 is ensured, the air in the chamber 12 is novel, and the ammonia concentration is prevented from exceeding 20 ppm.
Referring to fig. 1-3, on the basis that the box body 1 is provided with the exhaust fan 16, external light is prevented from irradiating the cavity 12 through a gap of the exhaust fan 16, which leads to failure of modeling of circadian rhythm disorder of the experimental mouse, therefore, a light shielding plate 17 is arranged in the cavity 12, wherein the light shielding plate 17 is arranged on one side wall with a vent, wherein a distance exists between the light shielding plate 17 and the exhaust fan 16, and the light shielding plate 17 is prevented from being blocked on the exhaust fan 16; more preferably, the length and width of the shutter 17 are greater than those of the vent; when the device is used, the direct-emitting point of the external light rays penetrating through the gap of the exhaust fan 16 always falls on the light shielding plate 17, so that the external light rays are prevented from damaging the night experiment environment simulated by the chamber 12.
In other embodiments of the present application, not shown, the box door 11 and the box body 1 are separately disposed, and a first groove and a second groove are disposed on an end surface of the box body 1 with an opening, wherein the first groove and the second groove are parallel, and two ends of the first groove and the second groove are not blocked, and the box door 11 enters the first groove and the second groove at one end of the first groove and the second groove, and slides towards the other end of the first groove and the second groove until the opening is completely blocked.
Referring to fig. 1 and 4, after the laboratory mice are bound, the laboratory mice are arranged on the rack 2, and then the rack 2 is placed in the chamber 12, so that, in the present embodiment, the rack 2 includes: a placing layer 21 and a support rod 22; the placing layer 21 has a first end face and a second end face, the placing area 24 is arranged on the first end face, the supporting rod 22 is arranged on the second end face, more specifically, the placing layer 21 is rectangular, the supporting rod 22 has a first rod, a second rod, a third rod and a fourth rod, and the same ends of the first rod, the second rod, the third rod and the fourth rod are all connected with the end face of the placing layer 21.
Referring to fig. 4-5, the placement layer 21 includes: frame, stainless steel strips 23; wherein, be the blank area in the middle of the frame, and in the blank area, longitudinal arrangement is provided with a plurality of parting beads, and the distance equals between each parting bead, stainless steel strip 23 sets up to a plurality of, and locate in the blank area, wherein, each stainless steel strip 23 is horizontal setting in the blank area, and each stainless steel strip 23 horizontal passes each parting bead, in addition, there is the distance between each stainless steel strip 23, in mouse experimentation, can make the produced excrement of experimental mouse, drop through blank area between the adjacent steel wire, so that to the centralized processing of experimental mouse excrement.
More specifically, the frame has a first edge and a second edge opposite to each other, wherein, a plurality of blocking members 25 are disposed along the first edge and the second edge of the frame and on each separating strip, and each blocking member 25 is parallel to each other, so that a placing area 24 is formed between adjacent blocking members 25, and the binding assembly is disposed between adjacent blocking members 25 and is limited by the blocking members 25.
Referring to fig. 4, in the present embodiment, the number of the dividing strips is set to 3, and the barrier 25 is set to 5, so that the frame has 4 placing areas 24, so as to place the bound experimental mice in batches, and after the modeling of the animal model is completed, the comparison between behaviors of different successfully modeled mice can be performed.
In this embodiment, the number of tie down assemblies and placement areas 24 is the same.
Referring to fig. 4 and fig. 6 to 7, in order to realize the restraint of the experimental mouse according to the body type of the experimental mouse, in the present embodiment, the restraint assembly includes:
binding plates 3 having the same width as the placement area 24, wherein the binding plates 3 are detachably disposed in the placement area 24, and there is one and only one binding plate 3 in each placement area 24; more specifically, the binding plate 3 has a first layer 31 and a second layer 32, and a first side wall and a second side wall are provided to connect the first layer 31 and the second layer 32, wherein the first layer 31 is provided with a chute.
And a plurality of locking assemblies 33 arranged in the sliding groove and moving radially with the sliding groove.
Referring to fig. 6-7, a plurality of spacers 34 disposed between the first layer 31 and the second layer 32, and each spacer 34 having one locking assembly 33 connected thereto, a bounding space 341 disposed between adjacent spacers 34, between the first sidewall and the spacer 34 nearest the first sidewall, and between the second sidewall and the spacer 34 nearest the second sidewall; wherein, when the locking assembly 33 moves in the sliding slot, the partition 34 moves synchronously with the locking assembly 33; causing the bound space 341 to increase or decrease; specifically, the partition plate 34 includes: a first partition 34 nearest to the first side and capable of fitting with the first side wall, a second partition 34 nearest to the second side wall and capable of fitting with the second side wall, and a plurality of third partitions 34 disposed between the first partition 34 and the second partition 34.
Referring to fig. 2, 6 and 9, a plurality of fixing members are inserted into the constrained space 341, and the width thereof is adaptively changed according to the increase or decrease of the constrained space 341; specifically, the first side wall, the second side wall, the first partition 34, the second partition 34 and the third partition 34 of the binding plate 3 are all provided with a clamping groove, wherein the clamping grooves are all located in the binding space 341, and two ends of a fixing member are respectively inserted into different clamping grooves, so that the movement range of the experimental mouse is limited, and the head and the tail of the experimental mouse are prevented from being exchanged towards the binding space 341; in addition, the head of the experimental mouse can be always oriented to the light bar 13.
The card slot includes a first card slot 342 and a second card slot 343, wherein the first card slot 342 and the second card slot 343 are both provided in plurality, and the first card slot 342 is close to the third sliding slot 313, and the second card slot 343 is close to the second sliding slot 312.
Referring to fig. 6, in the present embodiment, each of the plurality of fixing members includes: the first part is arranged in the second part, the second part is arranged in the first part, and the second part can partially extend out of the first part, so that the sum of the width of the first part and the extending width of the second part is always the same as the width of the binding space 341, more specifically, a cavity is arranged in the first part, the second part is arranged in the cavity, and at least one part of the second part does not separate from the cavity of the first part, wherein the cavity is provided with a through hole, the second part can extend out of the through hole, two opposite edges of the through hole are provided with blocking bulges, two opposite side walls of the second part are provided with limiting bulges, and the limiting bulges can not separate from the cavity of the first part all the time, when the second part continuously extends out of the first part, the limiting bulges gradually approach the blocking bulges until the two parts are contacted, through the arrangement, when the width of the binding space 341 is reduced or increased, the extending width of the second part in the first part of the fixing piece is adjusted, so that the fixing piece can be inserted in the binding space 341 all the time and can not separate from the binding space 341; thereby limiting the experimental mouse to the minimum range of motion.
Referring to fig. 9, preferably, a first spring is disposed in the cavity of the first portion, so that the second portion can always keep the cavity extending out of the first portion to the maximum extent, when the fixing device is used, the second portion is pressed, the first spring is pressed, then the fixing member is respectively inserted into the first clamping groove 342 and the second clamping groove 343, and meanwhile, the spring is expanded, so that the fixing member is always kept in the binding space 341.
Referring to fig. 6 and fig. 8 to 9, in the present embodiment, the fixing member includes: head mounting 35 and afterbody mounting 36, wherein, head mounting 35 is different with afterbody mounting 36, and the first part of afterbody mounting 36 is reserved has the tail groove, and in the back of pegging graft afterbody mounting 36 in first draw-in groove 342 second draw-in groove 343, the tail stretches out outside the constraint space 341 by the tail groove, and the cell wall of tail groove can extrude the restriction to the tail, and simultaneously, the setting of tail groove can also make things convenient for the natural downstream of mouse excrement.
Referring to fig. 2 and 6-8, when the restraint assembly is used, an experimental mouse is placed in the restraint space 341, the restraint space 341 can be adjusted to a minimum range according to the body type of the experimental mouse by moving the position of each partition 34, then the head fixing member 35 is inserted into the first card slot 342, the tail fixing member 36 is inserted into the second card slot 343, the head fixing member 35 is close to the head of the experimental mouse, the tail fixing member 36 is close to the tail of the experimental mouse, and when the restraint plate 3 is placed in the placement space, the head of the experimental mouse faces the light bar 13 to induce the disturbance of the circadian rhythm of the experimental mouse or reverse the cognition of the experimental mouse on the circadian rhythm, so as to construct a depression experimental mouse model accompanied with the circadian rhythm disturbance.
Referring to fig. 6-7, in this embodiment, the runner of the first layer 31 of the binding plate 3 comprises: a first chute 311, a second chute 312, and a third chute 313; the first sliding slot 311 is disposed between the second sliding slot 312 and the third sliding slot 313, and the locking component 33 is partially disposed on the first sliding slot 311, the second sliding slot 312, and the third sliding slot 313, and moves in the first sliding slot 311, the second sliding slot 312, and the third sliding slot 313.
Most preferably, the center line of the first sliding slot 311 is the same from the center line of the second sliding slot 312 and from the center line of the third sliding slot 313, and the center line of the first sliding slot 311 is parallel to the center line of the second sliding slot 312 and the center line of the third sliding slot 313.
Referring to fig. 6-8, in this embodiment, a plurality of locking assemblies 33 each include: a sliding part 4, which is partially disposed in the second sliding slot 312 and the third sliding slot 313 and is connected to a partition 34, wherein the sliding part 4 further has an accommodating cavity; specifically, the sliding member 4 has a first sliding portion and a second sliding portion, wherein the first sliding portion is located in the first sliding slot 311, the second sliding portion is located in the second sliding slot 312, the third sliding slot 313 is located between the first sliding portion and the second sliding portion, and the partition 34 is fixedly connected to the first sliding portion and the second sliding portion of the sliding member 4 by welding/bonding, so that when the sliding member 4 slides in the second sliding slot 312 and the third sliding slot 313, the partition 34 moves synchronously with the sliding member 4, thereby adjusting the bound space 341.
Preferably, the partition 34 is always perpendicular to the first layer 31 of the tie plate 3 during sliding.
Referring to fig. 7 and 11-13, retaining member 41; arranged in the accommodating cavity and the first chute 311, and has a locking state and a releasing state when in use; the locking member 41 has a third sliding portion, wherein the third sliding portion slides in the third sliding slot 313, the locking member 41 further has a first contact wall 411 and a second contact wall 412, wherein in the locked state, the first contact wall 411 and the second contact wall 412 are always pressed against the first layer 31 of the binding plate 3 to lock the position of the sliding member 4 carrying the partition 34 after moving, and in the released state, the first contact wall 411, the second contact wall 412 and the first layer 31 of the binding plate 3 have a gap, so that the sliding member 4 can slide in the second sliding slot 312 and the third sliding slot 313, thereby adjusting the width of the binding space 341.
More specifically, the locking member 41 has a first connecting portion and a second connecting portion, wherein the first connecting portion is provided with a first connecting groove, the second connecting portion is provided with a second connecting groove, the first connecting groove is opposite to the second connecting groove, and a spring groove is provided between the first connecting groove and the second connecting groove.
Referring to fig. 10-11, a button 42 is provided to actuate the locking member 41 between the locked and released states; the button 42 is partially disposed in the accommodating cavity and acts on the locking member 41, the button 42 has a third connecting groove and a fourth connecting groove, the third connecting groove corresponds to the first connecting groove, the fourth connecting groove corresponds to the second connecting groove, and when the button 42 is pressed, the button 42 can partially extend into the spring groove.
Referring to fig. 11-13, a rocker; what the wane pivoted set up in holding the intracavity for connect button 42 and retaining member 41, wherein, retaining member 41 is connected to the one end of wane, and button 42 is connected to the other end of wane, when button 42 moves, makes button 42 drive retaining member 41 through the wane, and is concrete, the wane includes: a first rocker 43 and a second rocker 44, wherein the first rocker 43 has a first end 431, a second end 432, and a first pivot 433, and the second rocker 44 has a third end 441, a fourth end 442, and a second pivot 443; the first end 431 of the first rocker 43 is located in the first connecting groove of the locking member 41, the second end 432 of the first rocker 43 is located in the third connecting groove of the button 42, the third end 441 of the second rocker 44 is located in the second connecting groove of the locking member 41, the fourth end 442 of the second rocker 44 is located in the fourth connecting groove of the button 42, the first end 431 and the second end 432 of the first rocker 43, and the third end 441 and the fourth end 442 of the second rocker 44 are both cylindrical, the first end 431 of the first rocker 43 is tangent to the groove wall of the first connecting groove, the second end 432 of the first rocker 43 is tangent to the groove wall of the third connecting groove, the third end 441 of the second rocker 44 is tangent to the groove wall of the second connecting groove, the fourth end 442 of the second rocker 44 is tangent to the groove wall of the fourth connecting groove, and the first pivot portion 433 and the second pivot portion 443 are pivotally disposed in the accommodating cavity.
Referring to fig. 12 to 13, when the button 42 is pressed, the second end 432 of the first rocker 43 and the fourth end 442 of the second rocker are in a descending state in the accommodating cavity, at this time, the first end 431 of the first rocker 43 and the third end 441 of the second rocker are in an ascending state in the accommodating cavity, and meanwhile, the locking member 41 is driven by the first end 431 of the first rocker 43 and the third end 441 of the second rocker to be in an ascending and descending state in the accommodating cavity, so that a gap is gradually generated between the first contact wall 411 and the second contact wall 412 of the locking member 41 and the first layer 31 of the binding plate 3, and at this time, the sliding member 4 can slide in the first sliding slot 311 and the second sliding slot 312; when the button 42 is pulled outwards, the second end 432 of the first tilting plate 43 and the fourth end 442 of the second tilting plate are in a raised state in the accommodating cavity, and at this time, the first end 431 of the first tilting plate 43 and the third end 441 of the second tilting plate are in a lowered state in the accommodating cavity, so that the first contact wall 411 and the second contact wall 412 of the locking member 41 gradually press against the first layer 31 of the binding plate 3, so that the first contact wall 411 and the second contact wall 412 are tightly attached to the first layer 31 of the binding plate 3, and at this time, the position of the sliding member 4 is locked.
Preferably, a second spring 45 is disposed in the spring slot of the locking member 41, wherein one end of the second spring 45 is connected to the locking member 41, the other end of the second spring 45 is connected to the button 42, under the action of the spring, the button 42 is always away from the spring, and meanwhile, when the first rocker 43 and the second rocker 44 drive the first contact wall 411 and the second contact wall 412 of the locking member 41 to always closely adhere to the first layer 31 of the binding plate 3, the second end 432 of the first rocker 43 and the fourth end 442 of the second rocker 44 are always located in the third connecting slot and the fourth connecting slot of the button 42, so as to prevent the button 42 from being disengaged from the sliding member 4; thereby keeping the locking member 41 in the locked state.
The use method of the invention comprises the following steps:
when the mouse binding box is used, a box door is opened, the box body is partially or completely separated from a cavity, then a binding plate is taken down from a placing area, a mouse used for an experiment is placed in a proper binding area, a locking piece is in a release state by pressing a button, a sliding piece is slid, the range of a binding space is adjusted according to the size of the body of the experiment mouse, a head fixing piece and a tail fixing piece are inserted into a first clamping groove and a second clamping groove, the head and the tail of the experiment mouse are bound, then the binding plate loaded with the experiment mouse is placed in the placing area of the box body, the box body is placed in the box body, then the box door is closed, and the lighting time and the illumination time length of a light strip are set through an operation key; meanwhile, the exhaust fan is always in a working state.
The above description of the embodiments is only for the understanding of the present invention. It should be noted that modifications can be made to the invention without departing from the principle of the invention, which will fall within the scope of the appended claims.
Claims (10)
1. A mouse chronic restraint stress model building device is characterized by comprising:
the mouse experiment device comprises a box body and a control module, wherein the box body is used for constructing an experiment environment of a mouse, the box body is provided with a chamber which is used for placing an experimental animal and is provided with an opening, and a box door which can completely cover the opening of the chamber, and the box door is movably connected to the box body or detachably connected to the box body;
the rack is configured in the cavity and used for bearing the restrained experimental mouse, and the rack is provided with at least one placing area;
the binding assembly is arranged in the placing area and used for binding the experimental mice, the number of the binding assemblies is at least one, the binding assembly is provided with a plurality of binding spaces for binding the experimental mice, each binding space can be relatively reduced or increased, and each binding space can be locked after being reduced or increased.
2. The mouse chronic restraint stress model building apparatus according to claim 1, wherein the rack body comprises: the device comprises a placing layer and a supporting rod, wherein the placing layer is provided with a first end face and a second end face, the placing area is arranged on the first end face, and the supporting rod is arranged on the second end face.
3. The device for modeling chronic restraint stress in mice of claim 2, wherein a barrier is disposed between two opposing edges of said first end of said placement layer and adjacent said placement area, wherein each of said barriers is parallel and each of said barriers is perpendicular to said first end of said placement layer.
4. The mouse chronic restraint stress model building apparatus of claim 1, wherein the number of restraint assemblies and placement areas is the same.
5. The mouse chronic restraint stress model building apparatus according to claim 1,
the tie down assembly includes: the binding plate is provided with a first layer and a second layer, a first side wall and a second side wall are arranged to connect the first layer with the second layer, and the first layer is provided with a sliding groove;
the locking assemblies are partially arranged in the sliding groove and move in the sliding groove in the radial direction;
the plurality of partition plates are arranged between the first layer and the second layer, each partition plate is connected with one locking assembly, and the bound spaces are arranged between the adjacent partition plates, between the first side wall and the partition plate closest to the first side wall and between the second side wall and the partition plate closest to the second side wall; when the locking assembly moves in the sliding groove, the partition plate moves synchronously with the locking assembly; causing the bound space to increase or decrease;
the fixing pieces are inserted into the bound space, and the width of the fixing pieces is changed in a self-adaptive mode along with the increase or decrease of the bound space.
6. The mouse chronic restraint stress model building apparatus according to claim 5, wherein the number of the locking assemblies and the number of the spacers are the same.
7. The mouse chronic restraint stress model establishing device according to claim 5, wherein the chute comprises: the first sliding chute, the second sliding chute and the third sliding chute; the first sliding groove is arranged between the second sliding groove and the third sliding groove, and the locking component is partially configured on the first sliding groove, the second sliding groove and the third sliding groove and moves in the first sliding groove, the second sliding groove and the third sliding groove.
8. The mouse chronic restraint stress model building apparatus according to claim 5, wherein a plurality of the fixing members each comprise: the first part and the second part are arranged in the first part, and the second part can partially protrude out of the first part, so that the sum of the width of the first part and the width of the second part is always the same as the width of a bound space.
9. The mouse chronic restraint stress model building apparatus according to claim 7,
a plurality of said locking assemblies each comprising: the sliding part is partially arranged in the second sliding groove and the third sliding groove and is connected with the partition board, and the sliding part is also provided with an accommodating cavity;
a locking member; the first sliding chute is arranged in the accommodating cavity and the first sliding chute and has a locking state and a releasing state when in use;
a button; part of the locking piece is arranged in the accommodating cavity and acts on the locking piece, and the locking piece can be driven to be switched between the locking state and the releasing state;
a seesaw; the button is pivotally arranged in the accommodating cavity and used for connecting the button and the locking piece, wherein one end of the rocker is connected with the locking piece, the other end of the rocker is connected with the button, and the button drives the locking piece through the rocker when the button moves;
a second spring; one end of the second spring is connected with the locking piece, and the other end of the second spring is connected with the button, so that the locking piece is always kept in the locking state.
10. The mouse chronic restraint stress model establishing device according to claim 9, wherein the locking member has a first contact wall and a second contact wall, wherein when the locking member is in the locking state, the first contact wall and the second contact wall are always pressed against the first layer of the restraint plate under the action of a second spring; the retaining member has a gap between the first contact wall, the second contact wall and the first layer of the tie plate in the released state.
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| CN202310213866.XA CN115918559B (en) | 2023-03-08 | 2023-03-08 | Mouse chronic constraint stress model establishment device |
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| CN115918559B (en) | 2023-06-16 |
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Address after: 100850 No. 27 Taiping Road, Beijing, Haidian District Patentee after: ACADEMY OF MILITARY MEDICAL SCIENCES Address before: 100039 27 Taiping Road, Haidian District, Beijing Patentee before: ACADEMY OF MILITARY MEDICAL SCIENCES |