CN114651782A - Method and device for establishing mouse chronic constraint stress model - Google Patents
Method and device for establishing mouse chronic constraint stress model Download PDFInfo
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- CN114651782A CN114651782A CN202210183616.1A CN202210183616A CN114651782A CN 114651782 A CN114651782 A CN 114651782A CN 202210183616 A CN202210183616 A CN 202210183616A CN 114651782 A CN114651782 A CN 114651782A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
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Abstract
The invention discloses a method and a device for establishing a mouse chronic constraint stress model, relates to the field of gastric cancer, and aims to solve the problem of stress response of the existing undefined chronic constraint on the mouse model. The scheme is that the device comprises a binding box body, wherein a first partition plate is fixedly connected to the left side and the right side of the inner wall of the binding box body. According to the invention, the motor, the first gear, the second gear and the threaded rod are arranged, so that the activity space of the mouse model can be well changed, the stress response of chronic constraint on the mouse model can be known, a quantifiable guiding strategy can be further provided for implementing psychological intervention treatment, psychological investigation, hormone level detection and various molecular biological methods are adopted for research, the research scheme fully embodies the close connection of the foundation and clinical research on the early-stage basis of consolidation, and a foundation is laid for discovering the observable premonitory index of the malignant progression of the gastric cancer.
Description
Technical Field
The invention relates to the field of gastric cancer, in particular to a method and a device for establishing a mouse chronic restraint stress model.
Background
At present, the clinical treatment means of the gastric cancer mainly comprises surgery combined with radiotherapy and chemotherapy, the influence of psychosocial factors on tumor patients is gradually known, especially experimental research proves that chronic psychological stress can accelerate tumor growth and promote malignant development of the tumor patients, so that the urgency of the psychological treatment of the tumor is increasingly concerned, but the action mechanism of the chronic psychological stress on the malignant development of the tumor is not clear, the clinical treatment only stays in the general psychological support stage of health promotion and education and the like of the patients at present, relatively accurate intervention treatment cannot be given, and the clinical treatment becomes a common problem in the fields of oncology and psychological research.
The method for establishing the functional dyspepsia mouse model with the patent number of CN108815706A comprises the following steps of randomly applying chronic unpredictable mild stress after sequentially applying restraint, electric shock and fatigue swimming to a mouse every day for 28-32 days, and randomly applying the chronic unpredictable mild stress after sequentially applying restraint, electric shock and fatigue swimming to the mouse so that an experimental animal shows the similar functional dyspepsia symptom to a clinical patient, and simultaneously, the stress intensity is moderate, no physical injury is caused to the experimental animal, and no organic change is caused, so that the method accords with the characteristic of functional dyspepsia.
However, the influence of psychosocial factors on the prognosis of tumor patients is receiving increasing attention, and research shows that chronic stress generated by a micro organism under psychological stress such as long-term anxiety and depression can promote malignant progression of tumors by acting on beta 2AR, and since plexinA1 and beta 2-AR have correlation in chronic stress regulation and control of malignant progression of gastric cancer, beta 2-AR influences gastric cancer cell EMT by activating STAT3 signal pathway, plexinA l influences gastric cancer tube generation by regulating VEGFR2, in conclusion, it is hypothesized that in gastric cancer patients, chronic stress can influence cancer cell ENT and angiogenesis once by mediating JAK-STAT3 pathway through beta 2-AR/plexinA1, and further promote malignant progression of gastric cancer, and the chronic stress phenomenon is ubiquitous in tumor patients and accompanied by significant increase of stress-related hormone level, in the prior art, the adrenal hormone secretion is stimulated around chronic stress in a mouse model and acts on corresponding receptors, further discussion of the lack of mechanism of chronic binding to promote tumor malignancy.
Therefore, the unclear chronic restriction on the stress response of mouse models is a technical problem that those skilled in the art are currently in need of solving.
Disclosure of Invention
Objects of the invention
In view of the above, the present invention provides a method and an apparatus for establishing a mouse chronic restraint stress model, so as to achieve the purpose of chronic restraint stress reaction of the mouse model.
(II) technical scheme
In order to achieve the technical purpose, in one aspect, the invention provides a device for establishing a mouse chronic restraint stress model, which comprises the following steps:
the binding box comprises a binding box body, wherein the left side and the right side of the inner wall of the binding box body are fixedly connected with a first partition plate together, the upper surface of the first partition plate is fixedly connected with a plurality of second partition plates which are uniformly distributed, the top end of the binding box body is provided with a plurality of first through openings which are uniformly distributed, the first partition plate is respectively provided with a plurality of second through openings which are uniformly distributed, and the top ends of the second through openings are respectively connected with a shielding mechanism in a sliding manner;
fixedly connected with third baffle is located at the lower surface center of first baffle, the remaining face of third baffle respectively with the inner wall side fixed connection of constraint box, the lower surface left and right sides of first baffle and the inner wall bottom left and right sides of constraint box be fixedly connected with sliding tray respectively, and is a plurality of two liang of relative, two sliding tray has the fourth baffle, two respectively through the common sliding connection of first sliding plate the lateral wall of fourth baffle is fixedly connected with pushing mechanism respectively, two the opposite side of fourth baffle has space adjustment mechanism through third sliding plate sliding connection respectively, can adjust the space in the constraint box through fourth baffle and first baffle to the convenience is carried out chronic constraint stress response test to the mouse model.
Preferably, the shielding mechanism comprises a first shielding plate, the lower surface of the first shielding plate is slidably connected with the top end of the second through hole, the upper surface of the first shielding plate is fixedly connected with a first hydraulic rod through a receiving rod, and the other end of the first hydraulic rod is fixedly connected with a second partition plate close to the first partition plate.
Preferably, pushing mechanism includes the catch bar, the one end of catch bar and the lateral wall fixed connection of fourth baffle, the other end fixedly connected with catch plate of catch bar, the catch plate rotates through the rotor plate and is connected with the threaded rod, the first gear of the other end fixedly connected with of threaded rod, first gear engagement has the second gear, the inner wall of second gear passes through shaft coupling fixedly connected with motor.
Preferably, the side wall of the motor is fixedly connected with the side edge of the outer wall of the bounding box body, the upper end and the lower end of the side wall of the second gear are respectively connected with output shaft protection rods in a sliding mode through a second sliding plate, and the other ends of the two output shaft protection rods are respectively fixedly connected with the outer wall of the bounding box body.
Preferably, first gear is located the outside of constraint box, the outer wall sliding connection of catch bar has the protection tube, the other end of protection tube and the inner wall side fixed connection of constraint box, the lateral wall of constraint box is seted up with the corresponding screw thread through-hole of threaded rod.
Preferably, the space adjusting mechanism comprises a telescopic sleeve plate, one side of the telescopic sleeve plate is connected with the side wall of the fourth partition plate in a sliding mode through a third sliding plate, a second hydraulic rod is fixedly connected to one side of the bottom of the telescopic sleeve plate, the other end of the second hydraulic rod is fixedly connected with the bottom end of the inner wall of the constraint box body, the other side of the telescopic sleeve plate is connected with the side wall of the third partition plate in a sliding mode, the space in the constraint box body can be adjusted better through the telescopic sleeve plate, and the reaction test to the mouse model is convenient.
Preferably, it is a plurality of the top of first opening is rotated through the pivot respectively and is connected with the second shielding plate, the outside bottom of constraint box is provided with gaseous filling tube, gaseous filling tube's outer wall is provided with the control valve, the fixed intercommunication in top of gaseous filling tube has a plurality of minutes pipes, and is a plurality of the top of minute pipe communicates with the bottom mounting of constraint box respectively, and is a plurality of the top of minute pipe is provided with the atomizer respectively.
Preferably, a plurality of the top of second baffle respectively with the inner wall top fixed connection of constraint box, a plurality of the second baffle respectively with adjacent first opening corresponding.
On the other hand, the invention also provides a method for establishing the mouse chronic restraint stress model, which comprises the device for establishing the mouse chronic restraint stress model, and the establishing steps are as follows:
step one, placing a mouse model into a bounding box body through a second baffle plate, and blocking the moving space of the mouse model through a second partition plate and a first partition plate;
step two, starting a motor, driving the positions of a pushing plate and a pushing rod to change through a second gear, a first gear and a threaded rod, so as to drive a fourth partition plate to slide in a sliding groove, starting a second hydraulic rod to work, and adjusting the size of a movable space through a telescopic sleeve;
step three, the first hydraulic rod starts to work, and the position of the first baffle plate is changed, so that the range of the moving space of the mouse model is changed, and the mouse model can move for a period of time in the moving spaces in different ranges;
and step four, opening the control valve, capturing the mouse model in the bounding box body through the gas injection pipe and the atomizing nozzle, and investigating the stress response of the mouse model in the moving spaces with different sizes.
According to the technical scheme, the method has the following beneficial effects:
1: according to the invention, the motor, the first gear, the second gear and the threaded rod are arranged, so that the activity space of the mouse model can be well changed, the stress response of chronic constraint on the mouse model can be known, a quantifiable guiding strategy can be provided for implementing psychological intervention treatment, psychological investigation, hormone level detection and various molecular biological methods are adopted for research, the research scheme fully embodies the close connection of the foundation and clinical research on the early-stage basis of consolidation, the expected result reveals the correlation between chronic stress and the clinical development of gastric cancer, and a foundation is laid for discovering the observable premonitory index of the malignant development of gastric cancer.
2: according to the invention, the output shaft of the motor can be well protected by the output shaft protection rod, so that the service time of the motor is effectively prolonged, the adjustment of the internal space of the constraint box body is facilitated, the activity space of a mouse model is well changed, and the stress response of chronic constraint on the mouse model is conveniently known.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a method and a device for establishing a mouse chronic restraint stress model provided by the invention.
FIG. 2 is a schematic diagram of a partial cross-sectional structure of a method and an apparatus for establishing a mouse chronic restraint stress model provided by the invention.
Fig. 3 is an enlarged schematic view of the structure at a in fig. 2.
Fig. 4 is an enlarged schematic view of the structure at B in fig. 2.
Fig. 5 is an enlarged view of the structure at C of fig. 2.
Fig. 6 is an appearance structure diagram of the second hydraulic rod and the telescopic sleeve plate on the left side and the fourth partition plate provided by the invention.
Description of the drawings: 1. binding the box body; 2. a second shielding plate; 3. a first separator; 4. a second separator; 5. a third partition plate; 6. a first hydraulic lever; 7. a first shielding plate; 8. a sliding groove; 9. a fourth separator; 10. a push rod; 11. a push plate; 12. a threaded rod; 13. a first gear; 14. a second gear; 15. a motor; 16. protecting the tube; 17. an output shaft guard bar; 18. a telescopic sleeve plate; 19. a second hydraulic rod; 20. a gas injection pipe; 21. an atomizing spray head; 22. and (4) controlling the valve.
Detailed Description
The following description is merely exemplary in nature and is not intended to limit the present disclosure, application, or uses. It should be understood that throughout the drawings, identical or similar reference numerals indicate identical or similar parts and features. The drawings are only schematic representations of the concepts and principles of embodiments of the disclosure, and do not necessarily show specific dimensions and proportions of the various embodiments of the disclosure. Certain features that are part of a particular figure may be exaggerated in order to illustrate relevant details or structures of embodiments of the present disclosure.
Referring to FIGS. 1-6:
example one
The utility model provides a device for establishing chronic constraint stress model of mouse, including constraint box 1, the first baffle 3 of the common fixedly connected with in inner wall left and right sides of constraint box 1, the last fixed surface of first baffle 3 is connected with a plurality of evenly distributed's second baffle 4, a plurality of evenly distributed's first opening is seted up on the top of constraint box 1, a plurality of evenly distributed's second opening is seted up respectively to first baffle 3, sliding connection respectively has the mechanism of sheltering from on the top of a plurality of second openings, can separate a plurality of chronic constraint stress models of mouse through second baffle 4 is fine, thereby can experiment.
Fixedly connected with third baffle 5 is located at the lower surface center of first baffle 3, the remaining face of third baffle 5 respectively with the inner wall side fixed connection who ties box 1, the lower surface left and right sides of first baffle 3 and the inner wall bottom left and right sides of tying box 1 fixedly connected with sliding tray 8 respectively, a plurality of sliding tray 8 are two liang relative, two sliding tray 8 respectively through the common sliding connection of first sliding plate have fourth baffle 9, the lateral wall of two fourth baffles 9 is fixedly connected with pushing mechanism respectively, the opposite side of two fourth baffles 9 has space adjustment mechanism through third sliding plate sliding connection respectively.
It should be noted that, the top of a plurality of first openings is connected with second shielding plate 2 through the pivot rotation respectively, restraint box 1's outside bottom is provided with gaseous injection pipe 20, gaseous injection pipe 20's outer wall is provided with control valve 22, the fixed intercommunication in top of gaseous injection pipe 20 has a plurality of minutes pipes, a plurality of tops of minute pipes respectively with restraint box 1's bottom mounting intercommunication, a plurality of tops of minute pipes are provided with atomizer 21 respectively, and, the top of a plurality of second baffles 4 respectively with restraint box 1's inner wall top fixed connection, a plurality of second baffles 4 are corresponding with the first opening that closes on respectively, can be fine through atomizer 21 inject into gas in the restraint box 1, through gaseous with the mouse model from the inboard only restraint box 1 outside of restraint box 1 of restraint box, thereby the convenience carries out data extraction to the mouse model.
Example two
The utility model provides a device for establishing chronic restraint stress model of mouse, it is on embodiment one's basis, pushing mechanism includes catch bar 10, the one end of catch bar 10 and the lateral wall fixed connection of fourth baffle 9, the other end fixedly connected with catch plate 11 of catch bar 10, catch plate 11 rotates through the rotor plate and is connected with threaded rod 12, the first gear 13 of the other end fixedly connected with of threaded rod 12, first gear 13 meshes has second gear 14, shaft coupling fixedly connected with motor 15 is passed through to the inner wall of second gear 14, can rotate through the first gear 13 of the fine drive of motor 15 and second gear 14, thereby it rotates to drive threaded rod 12.
In addition, the side wall of the motor 15 is fixedly connected with the side edge of the outer wall of the bounding box 1, the upper end and the lower end of the side wall of the second gear 14 are respectively connected with an output shaft protection rod 17 in a sliding way through a second sliding plate, and the other ends of the two output shaft protection rods 17 are respectively fixedly connected with the outer wall of the bounding box 1.
And, first gear 13 is located the outside of constraint box 1, and the outer wall sliding connection of catch bar 10 has protection tube 16, and the other end of protection tube 16 and the inner wall side fixed connection of constraint box 1, the lateral wall of constraint box 1 is seted up with the corresponding screw thread through-hole of threaded rod 12.
EXAMPLE III
The utility model provides a device for establishing chronic restraint stress model of mouse, it is on the basis of embodiment two, it includes first shielding plate 7 to shelter from the mechanism, the lower surface of first shielding plate 7 and the top sliding connection of second entrance, the upper surface of first shielding plate 7 is through accepting the first hydraulic stem 6 of pole fixedly connected with, the other end of first hydraulic stem 6 and the 4 fixed connection of second baffle that close on, can be through the better space separation that carries on of second baffle 4 to can carry out the restraint stress reaction test of different space sizes to the mouse model of difference.
It should be noted that the space adjusting mechanism includes a telescopic sleeve plate 18, one side of the telescopic sleeve plate 18 is connected with the side wall of the fourth partition plate 9 in a sliding manner through a third sliding plate, one side of the bottom of the telescopic sleeve plate 18 is fixedly connected with a second hydraulic rod 19, the other end of the second hydraulic rod 19 is fixedly connected with the bottom end of the inner wall of the bounding box body 1, and the other side of the telescopic sleeve plate 18 is connected with the side wall of the third partition plate 5 in a sliding manner.
Example four
A method for establishing a mouse chronic restraint stress model comprises the following establishing steps on the basis of the first embodiment to the third embodiment:
step one, a mouse model is placed in a constraint box body 1 through a second baffle plate 2, and the moving space of the mouse model is blocked through a second partition plate 4 and a first partition plate 3;
step two, starting a motor 15, driving the positions of a push plate 11 and a push rod 10 to change through a second gear 14, a first gear 13 and a threaded rod 12, so as to drive a fourth partition plate 9 to slide in a sliding groove 8, and a second hydraulic rod 19 starts to work, and the size of a moving space is adjusted through a telescopic sleeve 18;
step three, the first hydraulic rod 6 starts to work, and the position of the first baffle plate 7 is changed, so that the range of the activity space of the mouse model is changed, and the mouse model can move for a period of time in the activity space with different ranges;
and step four, opening the control valve 22, capturing the mouse model in the restraint box body 1 through the gas injection pipe 20 and the atomizing nozzle 21, and investigating the stress response of the mouse model in the activity spaces with different sizes.
The working principle is as follows: firstly, the rear side of a bounding box body 1 is parallel to the ground, then a second baffle plate 2 is moved, a plurality of mouse models are respectively placed into the bounding box body 1, then the second baffle plate 2 is reset, the mouse models are respectively separated by a plurality of second baffle plates 4, then two motors 15 respectively start to work, so as to drive a second gear 14 to rotate, and then drive a first gear 13 and a threaded rod 12 to start to rotate, in the process, an output shaft protection rod 17 slides on the side edge of the second gear 14, so as to protect an output shaft of the motor 15, the threaded rod 12 pushes a push rod 10 through a push plate 11, a fourth baffle plate 9 is driven by the push rod 10 to slide on a sliding groove 8 under the action of the sliding groove 8, and is protected by a protection pipe 16, so as to avoid the mouse models from contacting the threaded rod 12, and then a second hydraulic rod 19 starts to work, thereby driving the telescopic sleeve plate 18 to slide between the fourth partition plate 9 and the third partition plate 5, and then respectively adjusting the space sizes of the left side and the right side of the third partition plate 5.
Then the first hydraulic stem 6 of 4 lateral walls on the second baffle starts work to change the position of first shielding plate 7, then enlarge mouse model's activity space, and after the space of first baffle 3 below was adjusted, the active region scope of a plurality of mouse models differed, thereby can learn mouse model stress response under the constraint of different spaces.
Exemplary embodiments of the proposed solution of the present disclosure have been described in detail above with reference to preferred embodiments, however, it will be understood by those skilled in the art that many variations and modifications may be made to the specific embodiments described above, and that many combinations of the various technical features and structures presented in the present disclosure may be made without departing from the concept of the present disclosure, without departing from the scope of the present disclosure, which is defined by the appended claims.
Claims (9)
1. The device for establishing the mouse chronic restraint stress model comprises a restraint box body (1) and is characterized in that the left side and the right side of the inner wall of the restraint box body (1) are fixedly connected with a first partition plate (3) together, the upper surface of the first partition plate (3) is fixedly connected with a plurality of second partition plates (4) which are uniformly distributed, a plurality of first through openings which are uniformly distributed are formed in the top end of the restraint box body (1), a plurality of second through openings which are uniformly distributed are formed in the first partition plate (3) respectively, and the top ends of the second through openings are respectively connected with a shielding mechanism in a sliding mode;
fixedly connected with third baffle (5) are located at the lower surface center of first baffle (3), the remaining face of third baffle (5) respectively with the inner wall side fixed connection of constraint box (1), the lower surface left and right sides of first baffle (3) and the inner wall bottom left and right sides of constraint box (1) respectively fixedly connected with sliding tray (8), it is a plurality of sliding tray (8) two liang are relative, two sliding tray (8) have fourth baffle (9), two through the common sliding connection of first sliding plate respectively sliding tray (8) the lateral wall of fourth baffle (9) is fixedly connected with pushing mechanism respectively, two the opposite side of fourth baffle (9) has space adjustment mechanism through third sliding plate sliding connection respectively.
2. The device for establishing the mouse chronic restraint stress model according to claim 1, wherein the shielding mechanism comprises a first shielding plate (7), the lower surface of the first shielding plate (7) is slidably connected with the top end of the second opening, the upper surface of the first shielding plate (7) is fixedly connected with a first hydraulic rod (6) through a receiving rod, and the other end of the first hydraulic rod (6) is fixedly connected with an adjacent second partition plate (4).
3. The device for establishing the mouse chronic restraint stress model according to claim 1, wherein the pushing mechanism comprises a pushing rod (10), one end of the pushing rod (10) is fixedly connected with the side wall of the fourth partition plate (9), the other end of the pushing rod (10) is fixedly connected with a pushing plate (11), the pushing plate (11) is rotatably connected with a threaded rod (12) through a rotating plate, the other end of the threaded rod (12) is fixedly connected with a first gear (13), the first gear (13) is meshed with a second gear (14), and the inner wall of the second gear (14) is fixedly connected with a motor (15) through a coupler.
4. The device for establishing the mouse chronic restraint stress model according to claim 3, wherein the side wall of the motor (15) is fixedly connected with the side edge of the outer wall of the restraint box body (1), the upper end and the lower end of the side wall of the second gear (14) are respectively connected with an output shaft protection rod (17) in a sliding manner through a second sliding plate, and the other ends of the two output shaft protection rods (17) are respectively fixedly connected with the outer wall of the restraint box body (1).
5. The device for establishing the mouse chronic restraint stress model according to claim 3, wherein the first gear (13) is located on the outer side of the restraint box body (1), a protection pipe (16) is connected to the outer wall of the push rod (10) in a sliding manner, the other end of the protection pipe (16) is fixedly connected with the side edge of the inner wall of the restraint box body (1), and a threaded through hole corresponding to the threaded rod (12) is formed in the side wall of the restraint box body (1).
6. The device for establishing the mouse chronic restraint stress model according to claim 1, wherein the space adjusting mechanism comprises a telescopic sleeve plate (18), one side of the telescopic sleeve plate (18) is slidably connected with the side wall of the fourth partition plate (9) through a third sliding plate, one side of the bottom of the telescopic sleeve plate (18) is fixedly connected with a second hydraulic rod (19), the other end of the second hydraulic rod (19) is fixedly connected with the bottom end of the inner wall of the restraint box body (1), and the other side of the telescopic sleeve plate (18) is slidably connected with the side wall of the third partition plate (5).
7. The device for establishing the mouse chronic restraint stress model according to claim 1, wherein the top ends of the first through openings are respectively and rotatably connected with a second shielding plate (2) through a rotating shaft, the bottom end of the outer side of the restraint box body (1) is provided with a gas injection pipe (20), the outer wall of the gas injection pipe (20) is provided with a control valve (22), the top of the gas injection pipe (20) is fixedly communicated with a plurality of branch pipes, the top ends of the branch pipes are respectively and fixedly communicated with the bottom end of the restraint box body (1), and the top ends of the branch pipes are respectively provided with an atomizing nozzle (21).
8. The apparatus for establishing a mouse chronic restraint stress model according to claim 1, wherein the top ends of the second partition plates (4) are respectively fixedly connected with the top end of the inner wall of the restraint box body (1), and the second partition plates (4) respectively correspond to the adjacent first through openings.
9. A method for establishing a mouse chronic restraint stress model, which comprises an apparatus for establishing a mouse chronic restraint stress model as claimed in any one of claims 1 to 8, and comprises the following steps:
step one, a mouse model is placed into a bounding box body (1) through a second baffle plate (2), and the moving space of the mouse model is blocked through a second partition plate (4) and a first partition plate (3);
step two, starting a motor (15), driving the positions of a push plate (11) and a push rod (10) to change through a second gear (14), a first gear (13) and a threaded rod (12), so as to drive a fourth partition plate (9) to slide in a sliding groove (8), and a second hydraulic rod (19) starts to work, and the size of a movable space is adjusted through a telescopic sleeve (18);
step three, the first hydraulic rod (6) starts to work, and the position of the first baffle plate (7) is changed, so that the range of the moving space of the mouse model is changed, and the mouse model moves for a period of time in the moving spaces in different ranges;
and step four, opening the control valve (22), capturing the mouse model in the restraint box body (1) through the gas injection pipe (20) and the atomizing spray head (21), and investigating the stress response of the mouse model in the activity spaces with different sizes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210183616.1A CN114651782B (en) | 2022-02-28 | 2022-02-28 | Method and device for establishing chronic constraint stress model of mouse |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210183616.1A CN114651782B (en) | 2022-02-28 | 2022-02-28 | Method and device for establishing chronic constraint stress model of mouse |
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| Publication Number | Publication Date |
|---|---|
| CN114651782A true CN114651782A (en) | 2022-06-24 |
| CN114651782B CN114651782B (en) | 2023-05-16 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115250937A (en) * | 2022-08-24 | 2022-11-01 | 吉林大学 | Biological mouse experiment case |
| CN115918559A (en) * | 2023-03-08 | 2023-04-07 | 中国人民解放军军事科学院军事医学研究院 | Device for establishing mouse chronic constraint stress model |
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| CN210746617U (en) * | 2019-09-09 | 2020-06-16 | 宁波大学 | Space constraint mouse cage |
| CN214318210U (en) * | 2020-09-21 | 2021-10-01 | 南华大学附属南华医院 | Rat sleep deprivation combined constraint stress device |
| CN215868353U (en) * | 2021-09-15 | 2022-02-18 | 长沙医学院 | Mouse experiment observation box for stress disorder research based on photovoltaic sulfur sitagliptin treatment |
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| CN210746617U (en) * | 2019-09-09 | 2020-06-16 | 宁波大学 | Space constraint mouse cage |
| CN214318210U (en) * | 2020-09-21 | 2021-10-01 | 南华大学附属南华医院 | Rat sleep deprivation combined constraint stress device |
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| CN115250937A (en) * | 2022-08-24 | 2022-11-01 | 吉林大学 | Biological mouse experiment case |
| CN115250937B (en) * | 2022-08-24 | 2024-01-30 | 吉林大学 | Biological mouse experiment box |
| CN115918559A (en) * | 2023-03-08 | 2023-04-07 | 中国人民解放军军事科学院军事医学研究院 | Device for establishing mouse chronic constraint stress model |
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