CN1158830A - Natural plant fertilizer and its production - Google Patents
Natural plant fertilizer and its production Download PDFInfo
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- CN1158830A CN1158830A CN 97100688 CN97100688A CN1158830A CN 1158830 A CN1158830 A CN 1158830A CN 97100688 CN97100688 CN 97100688 CN 97100688 A CN97100688 A CN 97100688A CN 1158830 A CN1158830 A CN 1158830A
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- natural plant
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- amino acid
- sugar
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 30
- 238000004519 manufacturing process Methods 0.000 title claims description 13
- 150000001413 amino acids Chemical class 0.000 claims abstract description 23
- 241000233866 Fungi Species 0.000 claims abstract description 19
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 16
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 16
- 235000000346 sugar Nutrition 0.000 claims abstract description 16
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 claims abstract description 11
- 230000001603 reducing effect Effects 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 38
- 238000000034 method Methods 0.000 claims description 28
- 241000196324 Embryophyta Species 0.000 claims description 21
- 229910052757 nitrogen Inorganic materials 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 14
- 238000000855 fermentation Methods 0.000 claims description 12
- 230000004151 fermentation Effects 0.000 claims description 12
- 241000894006 Bacteria Species 0.000 claims description 10
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 8
- 239000011591 potassium Substances 0.000 claims description 8
- 229910052700 potassium Inorganic materials 0.000 claims description 8
- 239000004615 ingredient Substances 0.000 claims description 7
- 229910019142 PO4 Inorganic materials 0.000 claims description 6
- 230000008030 elimination Effects 0.000 claims description 6
- 238000003379 elimination reaction Methods 0.000 claims description 6
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 6
- 239000010452 phosphate Substances 0.000 claims description 6
- 235000012054 meals Nutrition 0.000 claims description 5
- 235000015099 wheat brans Nutrition 0.000 claims description 4
- 239000003995 emulsifying agent Substances 0.000 claims description 3
- 238000011081 inoculation Methods 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 238000012807 shake-flask culturing Methods 0.000 claims description 3
- 239000004202 carbamide Substances 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims description 2
- 238000003825 pressing Methods 0.000 claims description 2
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 claims description 2
- 235000010234 sodium benzoate Nutrition 0.000 claims description 2
- 239000004299 sodium benzoate Substances 0.000 claims description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N urea group Chemical group NC(=O)N XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 2
- 239000012535 impurity Substances 0.000 claims 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims 1
- 229920000053 polysorbate 80 Polymers 0.000 claims 1
- 239000002207 metabolite Substances 0.000 abstract description 8
- 201000010099 disease Diseases 0.000 abstract description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 6
- 239000002689 soil Substances 0.000 abstract description 6
- 241001465754 Metazoa Species 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 238000001914 filtration Methods 0.000 abstract description 4
- 235000015097 nutrients Nutrition 0.000 abstract description 3
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 abstract description 2
- 230000002349 favourable effect Effects 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 abstract description 2
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 abstract 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 abstract 1
- 235000019796 monopotassium phosphate Nutrition 0.000 abstract 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 abstract 1
- 239000000843 powder Substances 0.000 abstract 1
- 230000012010 growth Effects 0.000 description 7
- 230000008635 plant growth Effects 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 6
- 239000003053 toxin Substances 0.000 description 6
- 231100000765 toxin Toxicity 0.000 description 6
- 239000007921 spray Substances 0.000 description 4
- 229930006000 Sucrose Natural products 0.000 description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 230000001965 increasing effect Effects 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000000050 nutritive effect Effects 0.000 description 3
- 238000004321 preservation Methods 0.000 description 3
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 2
- 241000123649 Botryotinia Species 0.000 description 2
- 235000016623 Fragaria vesca Nutrition 0.000 description 2
- 240000009088 Fragaria x ananassa Species 0.000 description 2
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 239000003905 agrochemical Substances 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- 238000003912 environmental pollution Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000003895 organic fertilizer Substances 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000002798 spectrophotometry method Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 231100000167 toxic agent Toxicity 0.000 description 2
- 239000003440 toxic substance Substances 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- 239000003643 water by type Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- 241000123650 Botrytis cinerea Species 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 244000241235 Citrullus lanatus Species 0.000 description 1
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- CZMRCDWAGMRECN-UHFFFAOYSA-N Rohrzucker Natural products OCC1OC(CO)(OC2OC(CO)C(O)C(O)C2O)C(O)C1O CZMRCDWAGMRECN-UHFFFAOYSA-N 0.000 description 1
- 244000061458 Solanum melongena Species 0.000 description 1
- 235000002597 Solanum melongena Nutrition 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 125000003277 amino group Chemical group 0.000 description 1
- RJGDLRCDCYRQOQ-UHFFFAOYSA-N anthrone Chemical compound C1=CC=C2C(=O)C3=CC=CC=C3CC2=C1 RJGDLRCDCYRQOQ-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000005587 bubbling Effects 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 235000013681 dietary sucrose Nutrition 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 235000005489 dwarf bean Nutrition 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 239000010200 folin Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 239000005648 plant growth regulator Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002910 solid waste Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 230000008719 thickening Effects 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002912 waste gas Substances 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Landscapes
- Fertilizers (AREA)
Abstract
The pure natural plant nutrient fertilizer has its main components of protein, amino acid, non-protein and non-amino nitrogen, reducing sugar and abscisic acid as well as water. It is a metabolite of CGMCC0289 and is produced by using the said fungus as strain through three-stage culture for 14 days, filtering to remove mycelium, and concentration. The culture medium contains bran, sugar, orange peel powder and water, and preferably also contains potassium dihydrogen phosphate. The said fertilizer has good output-increasing and disease-resisting effect, no harm to human body and animal and no pollution to environment, and is favourable to protect soil.
Description
The present invention relates to organic fertilizer, more particularly, relate to a kind of natural plant fertilizer and manufacture method thereof.
At present, fertilizers such as the chemical fertilizer of nitrogenous, phosphorus, potassium and small portion animal excrement are mainly used in China farmland.But in order to reach the purpose of volume increase, will repeatedly use every year, and this is the labor force and cause very big burden to the peasant economically.Fertilizers such as animal excrement sources is limited, can cause soil texture deterioration even causes the underproduction and can cause environmental pollution and use chemical fertilizer in a large number.
The purpose of this invention is to provide and a kind ofly can overcome the various shortcomings of above-mentioned fertilizer, obvious effect of increasing production and can not cause the natural plant fertilizer and the manufacture method thereof of soil deterioration and environmental pollution.
The inventor is through deep research, found that, a kind of from the field ill crop gather, name after separation and the cultivation is called Fu Shi grapestone cup fungi [the Latin formal name used at school is Botryotinia fucheliana], its asexual generation is Botrytis cinerea Pers.] fungi can secrete a kind of a large amount of protein that contain, amino acid, the non-amino acid nitrogen of non-albumen, reducing sugar and dormin (Abscisic acid, abbreviation ABA) meta-bolites, protein wherein, amino acid, non-amino acid nitrogen of non-albumen and reducing sugar all are the nutrient substances that very helps plant-growth, and it can satisfy the needs of plant-growth to nitrogen fully.And dormin (below 1ppm) under the condition at the extremely low dense end is a kind of plant-growth regulator efficiently, it not only can promote significantly in all each stages of plant plant growth, bloom and solid, and can promote absorption and the increase disease resistance of plant of plant to nutritive elements such as the nitrogen in the soil, phosphorus, potassium, therefore, use the meta-bolites of Fu Shi grapestone cup fungi to plant, can save other fertilizer significantly.Yet the inventor also finds, if Fu Shi grapestone cup fungi incubation time surpassed for 2 weeks, can produce a kind of toxic substance that is called toxin, and this toxin is the growth of unfavorable sub-plant not only, also can make plant produce disease.The inventor thinks that Here it is causes Fu Shi grapestone cup fungi not to be used as the major cause of vegetable fertilizer for a long time.The inventor also finds after through further investigation, as long as total fermented incubation time of Fu Shi grapestone cup fungi is controlled in 7-14 days the scope, after finishing fermentation, take the several major measure such as mycelium elimination of the strainer of 74 μ m (200 order) with Fu Shi grapestone cup fungi, promptly can avoid the generation of toxin, only stay the composition useful, thereby become a kind of ten minutes natural plant fertilizer efficiently plant-growth.Because above-mentioned achievement in research has so far just been finished the present invention.
In order to obtain the bacterial strain of more effective Fu Shi grapestone cup fungi, the inventor has carried out the separation and the cultivation of bacterial strain.Its method is to suffer from the strawberry fruit of Fu Shi grapestone cup fungi after gather in the field, clean with sterilized water, be placed in the glass jar of thieving paper insulation then 48 hours, promptly selecting the fruit that has mycelia after waiting to find to grow mycelia on the disease fruit is placed in the culture dish that potato sucrose nutrient agar (abbreviation PDA substratum) is housed, after treating to grow mycelia in the culture dish, selecting the fresh healthy and strong mycelia that grows is placed in the PDA slant tube, after waiting to cover with mycelia, check the form of mycelia, gather monospore, colourless, the cultivation breeding that the oval spore of the botryoidalis of bunchiness enlarges.The result shows that the nutritive ingredient of this bacterial strain meta-bolites is very high, can be used as natural phant nutrient substance fertilizer and uses.For with this bacterial classification prolonged preservation, the inventor is sent to China Committee for Culture Collection of Microorganisms common micro-organisms center (being called for short CGMCC) preservation with it, and preservation date is on February 4th, 1997, and preserving number is CGMCCNo.0289.Classification called after Botryotinia fucheliana (Fu Shi grapestone cup fungi) abbreviates Fu Shi grapestone cup fungi CGMCC0289 as.
Thereby, the invention provides a kind of natural plant fertilizer, it is characterized in that it contains following ingredients:
The composition weight part
Protein 1000-1500
Amino acid 300-500
The non-amino acid nitrogen 85000-120000 of non-albumen
Reducing sugar 300-500
Dormin 2-5
Below, natural plant fertilizer of the present invention is explained in detail.
Protein in the mentioned component only is the different of its molecular weight size with amino acid whose difference, and protein is meant the complex body of multiple amino acids, just becomes amino acid after its hydrolysis, and in the present invention, the two is to distinguish by the measurement result of analytical procedure.The above-mentioned non-amino acid nitrogen of non-albumen is meant by the total nitrogen analytical results and deducts remaining nitrogen amount behind the nitrogen in protein and the amino acid.Because can synthesize a large amount of non-protein nitrogen(NPN) in the metabolic process of Fu Shi grapestone cup fungi, its structure is very complicated, therefore this a part of nitrogen is not made further analysis of the molecular structure.Above-mentioned reducing sugar is meant the carbohydrate with reducing property, wherein is mainly glucose.
The analytical procedure of above-mentioned each composition is as follows:
1, protein: with FOLIN phenol method, make the protein standard curve, produce UV-120-02 type spectrophotometric determination with Japan with bovine serum albumin.It is soluble protein fraction.
2, amino acid: producing Hitachi's automatic analyzer for amino acids with Japan and measure, is the free amine group acid constituents.
3, the non-amino acid nitrogen of non-albumen is measured with Na Shi reagent spectrophotometer method.
4, reducing sugar: use anthrone method, with spectrophotometric determination.
5, dormin: measure with SP-501N type gas chromatographicanalyzer.
Natural plant fertilizer of the present invention is the meta-bolites of Fu Shi grapestone cup fungi CGMCC0289, but thalline is wherein separated fully by the strainer of 74 μ m (200 order), therefore wherein there is not the secreted toxin of growth later stage of this mycelia, only keeps the nutritive ingredient favourable plant-growth.In addition, can preserve more steadily in the long term, add the emulsifying agent of minute quantity and stablizer (for example polysorbas20-80 and urea etc.) and sanitas (for example Sodium Benzoate etc.) in can the product after concentrating in order to make product of the present invention.
Should be noted that, though the fertilizer formula of the invention described above is the invention of making on the basis of research Fu Shi grapestone cup fungi meta-bolites, because the inventor has found that the combination of above-mentioned 5 kinds of compositions has very good plant-growth facilitation effect.Therefore, all belong to protection scope of the present invention as long as meet the Ru 2006101161 of above-mentioned 5 kinds of composition proportion.
In addition, the present invention also provides a kind of manufacture method of natural plant fertilizer, it is characterized in that,
(1) original strain is Fu Shi grapestone cup fungi CGMCC0289;
(2) prescription of substratum is:
Composition weight %
Generally preferred
Wheat bran 3-10 5-8
Sugar 1-5 1.5-3
Dry orange meal 0.5-4 0.5-1
Potassium primary phosphate 0.1-0.4
The water surplus surplus
(3) technical process is:
Original strain move bacterium → shake-flask culture first class inoculum-→ seed tank culture second class inoculum → ferment tank → elimination mycelium [74 μ m-53 μ m (200 orders-270 order)] → concentrate (below 60 ℃) → can;
(4) operational condition of technical process is:
Inoculation bacterium amount: 3-10 volume %;
Leavening temperature: 20-30 ℃, preferred 25-28 ℃;
PH value: 4-6.5, preferred 4.5-5.5;
Air flow: 0.12-0.25V/V/min;
Vibration (rotation) speed: 100-120 time/min;
Fermentation time: every grade 2.5-4 days, preferred 3-3.5 days; Three grades are no more than 14 days altogether, preferably are no more than 12 days.
Below the manufacture method of natural plant fertilizer of the present invention is explained in detail.
The suitable storage temperature of above-mentioned Fu Shi grapestone cup fungi is 0-4 ℃, but storage temperature does not belong to the prerequisite of the inventive method.Sugar in above-mentioned culture medium prescription can be table sugar, for example sucrose, beet sugar, glucose and maltose etc.In the prescription of above-mentioned substratum, potassium primary phosphate does not belong to neccessary composition, but the potassium primary phosphate of adding 0.1-0.4% can obtain result preferably.The operational condition of three grades of cultivations is basic identical in above-mentioned technical process.Wherein, leavening temperature is important condition, if temperature is lower than 20 ℃, then fermenting speed is too slow, if temperature surpasses 30 ℃, then easy bacteria infection descends quality product.Therefore now be decided to be 20-30 ℃, preferred 25-28 ℃.The pH value also is an essential condition, and the pH value is too high or too low all unfavorable to mycelial growth, but under normal fermentation condition, fermented liquid itself can maintain the pH value in the scope of 4-6.5, be preferably 4.5-5.5, along with the progress of fermenting process, the pH value has the trend of step-down gradually.Used gas is air during ventilation, because air draws materials easily, and wherein contains and enough is required oxygen and the nitrogen of hypha fermentation.Certainly, artificial nitrogen and the oxygen gas mixture that cooperates also is fine, but cost is too high like this.And used air preferably filters through air filter, to remove other assorted bacterium.In air filter, adopt gac and cotton to make stopping composition, airborne assorted bacterium and dust are disposed.Air flow is important condition, because must provide enough oxygen and nitrogen to mycelia.If guarantee that fermentor tank volume and bubbling air amount are 0.12-0.25V/V/min, promptly can satisfy the needs of the good gas growth of this bacterium.For deep layer in the jar and top layer mycelial growth temperature and ventilation evenly, essential additional mechanical stirs, the rotating speed of agitator is generally 100-120 rev/min.Fermentation time is important condition, fermentation time is too short, can not obtain enough mycelia amounts, be that mycelia does not reach the growth most productive period, the metabolite secretory volume is few, but fermentation time is long, the total time of three grade fermemtation was above 14 days, will make the mycelia that occurs some growth later stages in the system, these old mycelia can secrete the toxin that to be unfavorable for plant-growth in fermented liquid, and quality product is descended.Therefore every grade of fermentation time is defined as 2.5-4 days, preferred 3-3.5 days; Consider transit time at different levels, the total time of regulation three grade fermemtation is no more than 14 days, preferably is no more than 12 days.In addition, in the mycelial step of the elimination of technical process, mesh is the thickest to be 74 μ m (200 orders), if be coarser than 74 μ m, then can not guarantee to remove fully mycelium, its result, and the mycelium that leaked will secrete toxin at later stages.If mesh is less than 53 μ m (270 order), then filtration velocity is too slow.Be used for filtering equipment and be not particularly limited, but preferably use plate-and-frame filter press, can accelerate filtration velocity like this.As long as according to method proper operation of the present invention, just can make protein, amino acid, the non-amino acid nitrogen of non-albumen, reducing sugar, five kinds of components in proportions of dormin in the product meet the scope of stipulating in the natural plant fertilizer of the present invention.For the ease of storing and transportation, in the final step of technical process, concentrated and can, spissated multiple does not have strict regulation, generally be that its volume is concentrated into 1/3 of original volume, can make the protein content in the product reach 1000-1500mg/kg like this, all the other component contents are analogized according to the above ratio.During use,, promote crop disease-resistant and volume increase as long as with its dilute with water 200-250 doubly, promptly can be used for foliage-spray, the seed soaking of plant.
Compare with chemical fertilizer, organic fertilizer or other bacterial manure of prior art, product of the present invention has following advantage:
1, it is the meta-bolites of a kind of natural fungi, does not wherein contain the fermentation thalline, does not contain any toxic substance, and is harmless to people, animal.
2, do not contain any mineral compound, can protect the soil superperformance, and farm crop are had the function of volume increase and enhancing resistance against diseases two aspects.Use this product can use chemical fertilizer and the agricultural chemicals of 30-40% at least less.Both reduced production cost, protected soil fertility again and also reduced chemical fertilizer to a great extent and agricultural chemicals to the harm of human health.
3, easy to use.Use other bacterial fertilizer products repeatedly to spray, and this product only need use once, can make plant yield-increasing, strengthen resistance against diseases.And the saving of labor, save time, save cost.
4, do not have waste water, waste gas and solid waste in process of production and discharge environmental sound.
5, production technique is simple, and is with low cost.
Explain the present invention further below in conjunction with embodiment.
Embodiment 1
The substratum that uses is:
Composition weight %
Wheat bran 5
Sugar 2
Dry orange meal 0.5
Potassium primary phosphate 0.2
Water surplus
The operational condition of technical process is:
Inoculation bacterium amount: shake-flask culture 5 volume %; Seed tank culture and fermentor cultivation are all 10 volume %;
Leavening temperature: 25 ℃;
PH value: reduce to 4.5 gradually by 6.5;
Air flow: 0.20V/V/min;
Fermentation time: every grade 3 days, three grades 10 days altogether;
Elimination mycelium: sheet frame pressing machine, mesh 74 μ m (200 order);
Thickening temperature: 45 ℃;
Cycles of concentration: be concentrated into 1/3 of fermented liquid.
Each component content in the product:
Ingredient m g/kg
Protein 1000
Amino acid 300
The non-amino acid nitrogen 85000 of non-albumen
Reducing sugar 350
PBI 58 3
Other are mainly moisture.With 200 times of this leavened prod dilute with waters, spray paddy rice, wheat, several crops of rape, effect of increasing production 15-25%.
Embodiment 2
Except the substratum that uses as the following ingredients ratio, other conditions are identical with embodiment 1.
Composition weight %
Wheat bran 8
Sugar 1.5
Dry orange meal 1
Potassium primary phosphate 0.3
Water surplus
Each component content in the product:
Ingredient m g/kg
Protein 1200
Amino acid 500
The non-amino acid nitrogen 120000 of non-albumen
PBI 58 4
Other are mainly moisture.With 250 times of this leavened prod dilute with waters, spray crops such as string bean, soybean, watermelon, strawberry, eggplant, units increased in production is 20-42%.
Claims (8)
1, a kind of natural plant fertilizer is characterized in that, it contains following ingredients:
The composition weight part
Protein 1000-1500
Amino acid 300-500
The non-amino acid nitrogen 85000-120000 of non-albumen
Reducing sugar 300-500
Dormin 2-5
2, natural plant fertilizer as claimed in claim 1 is characterized in that, wherein also contains to be useful on to promote required emulsifying agent, stablizer and the sanitas of this product stability.
3, natural plant fertilizer as claimed in claim 2 is characterized in that, wherein said emulsifying agent is polysorbas20 and/or tween 80, and wherein said stablizer is a urea, and wherein said sanitas is a Sodium Benzoate.
4, a kind of manufacture method of natural plant fertilizer is characterized in that,
(1) original strain is Fu Shi grapestone cup fungi CGMCC0289;
(2) prescription of substratum is:
Composition weight %
The anti-3-10 of bran
Sugar 1-5
Dry orange meal 0.5-4
Water surplus
(3) technical process is:
Original strain move bacterium → shake-flask culture first class inoculum → seed tank culture second class inoculum → ferment tank → elimination mycelium [74 μ m-53 μ m (200 orders-270 order)] → concentrate (below 60 ℃)-→ can;
(4) operational condition of technical process is:
Inoculation bacterium amount: 3-10 volume %;
Leavening temperature: 20-30 ℃
PH value: 4-6.5
Air flow: 0.12-0.25V/V/min;
Vibration (rotation) speed: 100-120 time/min;
Fermentation time: every grade 2.5-4 days, three grades are no more than 14 days altogether.
5, method as claimed in claim 4 is characterized in that, wherein the prescription of said substratum is:
Composition weight %
Wheat bran 5-8
Sugar 1.5-3
Dry orange meal 0.5-1
Potassium primary phosphate 0.1-0.4
Water surplus
As claim 4 or 5 described methods, it is characterized in that 6, the leavening temperature in the wherein said technical process operational condition is 25-28 ℃; The pH value is 4.5-5.5; Fermentation time is: every grade 3-3.5 days, three grades are no more than 12 days altogether.
7, method as claimed in claim 4 is characterized in that, the mycelial sheet frame pressing machine of manipulating of wherein said elimination carries out.
8, method as claimed in claim 4 is characterized in that, the air that uses in said three grades of cultivations carries out the removal of impurities bacterium through air filter and filters.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97100688A CN1049646C (en) | 1997-03-13 | 1997-03-13 | Natural plant fertilizer and its production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN97100688A CN1049646C (en) | 1997-03-13 | 1997-03-13 | Natural plant fertilizer and its production |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1158830A true CN1158830A (en) | 1997-09-10 |
| CN1049646C CN1049646C (en) | 2000-02-23 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN97100688A Expired - Fee Related CN1049646C (en) | 1997-03-13 | 1997-03-13 | Natural plant fertilizer and its production |
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| Country | Link |
|---|---|
| CN (1) | CN1049646C (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101857498A (en) * | 2010-06-07 | 2010-10-13 | 中国农业科学院果树研究所 | Facility grape foliar fertilizer and spraying method |
| CN101913960A (en) * | 2010-07-24 | 2010-12-15 | 周冠旻 | Plant nutrition liquid fertilizer and preparation method thereof |
| CN102229512A (en) * | 2011-04-12 | 2011-11-02 | 郭宜夫 | Natural plant nutrient |
| CN105399532A (en) * | 2015-12-21 | 2016-03-16 | 上海永通化工有限公司 | Water-soluble amino acid fertilizer and preparation method thereof |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1131135A (en) * | 1994-10-06 | 1996-09-18 | 北京北南生化制品有限公司 | Pure natural plant nutrient extract-zhenjunwangfei and its preparation method |
-
1997
- 1997-03-13 CN CN97100688A patent/CN1049646C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101857498A (en) * | 2010-06-07 | 2010-10-13 | 中国农业科学院果树研究所 | Facility grape foliar fertilizer and spraying method |
| CN101913960A (en) * | 2010-07-24 | 2010-12-15 | 周冠旻 | Plant nutrition liquid fertilizer and preparation method thereof |
| CN101913960B (en) * | 2010-07-24 | 2013-02-20 | 周冠旻 | Plant nutrition liquid fertilizer and preparation method thereof |
| CN102229512A (en) * | 2011-04-12 | 2011-11-02 | 郭宜夫 | Natural plant nutrient |
| CN105399532A (en) * | 2015-12-21 | 2016-03-16 | 上海永通化工有限公司 | Water-soluble amino acid fertilizer and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1049646C (en) | 2000-02-23 |
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Owner name: SUZHOU CITY DUOLVDUO HIGH-TECH FERTILIZER CO., LT Free format text: FORMER OWNER: ZHANG YUANSHOU Effective date: 20030808 |
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Effective date of registration: 20030808 Address after: 215129 Jiangsu Province, Suzhou City District Ma Jian Lu Feng Qiao yuan 500 meters forward Patentee after: Suzhou Duolvduo High-tech Fertilizers Co., Ltd. Address before: 100028 Room 502 (north south company), main building of environmental sanitation building, still building, Chaoyang District, Beijing Patentee before: Zhang Yuanshou |
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