CN115873142A - Application of houttuynia polysaccharide extract in preparation of sleep improving medicines and health products - Google Patents
Application of houttuynia polysaccharide extract in preparation of sleep improving medicines and health products Download PDFInfo
- Publication number
- CN115873142A CN115873142A CN202211138881.4A CN202211138881A CN115873142A CN 115873142 A CN115873142 A CN 115873142A CN 202211138881 A CN202211138881 A CN 202211138881A CN 115873142 A CN115873142 A CN 115873142A
- Authority
- CN
- China
- Prior art keywords
- houttuynia cordata
- polysaccharide
- houttuynia
- polysaccharide extract
- sleep
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 57
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 57
- 240000000691 Houttuynia cordata Species 0.000 title claims abstract description 56
- 150000004676 glycans Chemical class 0.000 title claims abstract description 56
- 239000000284 extract Substances 0.000 title claims abstract description 31
- 239000003814 drug Substances 0.000 title claims abstract description 22
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
- 235000013717 Houttuynia Nutrition 0.000 title abstract description 17
- 229940079593 drug Drugs 0.000 title abstract description 12
- 230000036541 health Effects 0.000 title description 4
- 235000013719 Houttuynia cordata Nutrition 0.000 claims abstract description 54
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 14
- 235000013305 food Nutrition 0.000 claims abstract description 12
- 238000001556 precipitation Methods 0.000 claims abstract 2
- 239000002244 precipitate Substances 0.000 claims description 13
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 238000000605 extraction Methods 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 5
- 239000008194 pharmaceutical composition Substances 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- 238000010298 pulverizing process Methods 0.000 claims description 4
- 238000005406 washing Methods 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims 1
- 238000002604 ultrasonography Methods 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 17
- 241000758769 Houttuynia Species 0.000 abstract description 16
- 238000002474 experimental method Methods 0.000 abstract description 9
- 241001465754 Metazoa Species 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract 1
- 238000003809 water extraction Methods 0.000 abstract 1
- 241000699670 Mus sp. Species 0.000 description 15
- 238000011160 research Methods 0.000 description 9
- QGMRQYFBGABWDR-UHFFFAOYSA-M Pentobarbital sodium Chemical compound [Na+].CCCC(C)C1(CC)C(=O)NC(=O)[N-]C1=O QGMRQYFBGABWDR-UHFFFAOYSA-M 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 229960001412 pentobarbital Drugs 0.000 description 6
- 230000006872 improvement Effects 0.000 description 4
- 230000004620 sleep latency Effects 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 3
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 235000019441 ethanol Nutrition 0.000 description 3
- 206010022437 insomnia Diseases 0.000 description 3
- 238000012827 research and development Methods 0.000 description 3
- 208000019116 sleep disease Diseases 0.000 description 3
- 230000004617 sleep duration Effects 0.000 description 3
- UAJTZZNRJCKXJN-UHFFFAOYSA-M sodium;2-dodecoxy-2-oxoethanesulfonate Chemical compound [Na+].CCCCCCCCCCCCOC(=O)CS([O-])(=O)=O UAJTZZNRJCKXJN-UHFFFAOYSA-M 0.000 description 3
- 239000011550 stock solution Substances 0.000 description 3
- 241000712461 unidentified influenza virus Species 0.000 description 3
- 239000000341 volatile oil Substances 0.000 description 3
- 206010007247 Carbuncle Diseases 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 208000004852 Lung Injury Diseases 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 241000758742 Saururaceae Species 0.000 description 2
- 241000700584 Simplexvirus Species 0.000 description 2
- 208000007271 Substance Withdrawal Syndrome Diseases 0.000 description 2
- 208000033809 Suppuration Diseases 0.000 description 2
- 206010069363 Traumatic lung injury Diseases 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 229940049706 benzodiazepine Drugs 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- -1 coatings Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000006297 dehydration reaction Methods 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- HYBBIBNJHNGZAN-UHFFFAOYSA-N furfural Chemical compound O=CC1=CC=CO1 HYBBIBNJHNGZAN-UHFFFAOYSA-N 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 231100000515 lung injury Toxicity 0.000 description 2
- 239000000546 pharmaceutical excipient Substances 0.000 description 2
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 2
- 230000001376 precipitating effect Effects 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 230000004622 sleep time Effects 0.000 description 2
- 208000020685 sleep-wake disease Diseases 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- SVUOLADPCWQTTE-UHFFFAOYSA-N 1h-1,2-benzodiazepine Chemical compound N1N=CC=CC2=CC=CC=C12 SVUOLADPCWQTTE-UHFFFAOYSA-N 0.000 description 1
- NOEGNKMFWQHSLB-UHFFFAOYSA-N 5-hydroxymethylfurfural Chemical compound OCC1=CC=C(C=O)O1 NOEGNKMFWQHSLB-UHFFFAOYSA-N 0.000 description 1
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 229920002134 Carboxymethyl cellulose Polymers 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 206010012289 Dementia Diseases 0.000 description 1
- 206010012335 Dependence Diseases 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- 208000019693 Lung disease Diseases 0.000 description 1
- YJPIGAIKUZMOQA-UHFFFAOYSA-N Melatonin Natural products COC1=CC=C2N(C(C)=O)C=C(CCN)C2=C1 YJPIGAIKUZMOQA-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 206010035664 Pneumonia Diseases 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- 241000315672 SARS coronavirus Species 0.000 description 1
- 241000235342 Saccharomycetes Species 0.000 description 1
- 241000191967 Staphylococcus aureus Species 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 210000000683 abdominal cavity Anatomy 0.000 description 1
- 239000003070 absorption delaying agent Substances 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000002587 anti-hemolytic effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 239000000935 antidepressant agent Substances 0.000 description 1
- 229940005513 antidepressants Drugs 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000006286 aqueous extract Substances 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- 229940125717 barbiturate Drugs 0.000 description 1
- 150000001557 benzodiazepines Chemical class 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 235000010948 carboxy methyl cellulose Nutrition 0.000 description 1
- 239000008112 carboxymethyl-cellulose Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000000546 chi-square test Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 230000024203 complement activation Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- AAOVKJBEBIDNHE-UHFFFAOYSA-N diazepam Chemical compound N=1CC(=O)N(C)C2=CC=C(Cl)C=C2C=1C1=CC=CC=C1 AAOVKJBEBIDNHE-UHFFFAOYSA-N 0.000 description 1
- 229960003529 diazepam Drugs 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000007884 disintegrant Substances 0.000 description 1
- 239000002612 dispersion medium Substances 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- 239000003937 drug carrier Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 230000000517 effect on sleep Effects 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002212 flavone derivatives Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 235000021384 green leafy vegetables Nutrition 0.000 description 1
- 150000002402 hexoses Chemical class 0.000 description 1
- RJGBSYZFOCAGQY-UHFFFAOYSA-N hydroxymethylfurfural Natural products COC1=CC=C(C=O)O1 RJGBSYZFOCAGQY-UHFFFAOYSA-N 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 208000037817 intestinal injury Diseases 0.000 description 1
- 238000010255 intramuscular injection Methods 0.000 description 1
- 239000007927 intramuscular injection Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 239000000314 lubricant Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 229960003987 melatonin Drugs 0.000 description 1
- DRLFMBDRBRZALE-UHFFFAOYSA-N melatonin Chemical compound COC1=CC=C2NC=C(CCNC(C)=O)C2=C1 DRLFMBDRBRZALE-UHFFFAOYSA-N 0.000 description 1
- 230000004630 mental health Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000001543 one-way ANOVA Methods 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 229960002275 pentobarbital sodium Drugs 0.000 description 1
- 150000002972 pentoses Chemical class 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000035755 proliferation Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 230000002685 pulmonary effect Effects 0.000 description 1
- 230000001047 pyretic effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000028527 righting reflex Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000007492 two-way ANOVA Methods 0.000 description 1
- 238000002137 ultrasound extraction Methods 0.000 description 1
- 238000011870 unpaired t-test Methods 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
- 230000002618 waking effect Effects 0.000 description 1
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to a houttuynia cordata polysaccharide extract and a preparation method thereof, and application of the houttuynia cordata polysaccharide extract in preparation of a medicine or food for improving sleep. The houttuynia polysaccharide extract is prepared by using houttuynia as a main raw material according to a water extraction and alcohol precipitation method, has the effect of improving sleep as proved by animal experiments, and can be used for preparing medicines or foods for improving sleep.
Description
Technical Field
The invention relates to an application of houttuynia cordata polysaccharide extract in preparing medicines or foods, in particular to an application in preparing medicines or foods for improving sleep.
Background
Sleep takes almost a third of a lifetime. It is thought that sufficient sleep is of great importance to physiology. However, as the pressure of living continues to increase, ensuring "regular" sleep at 7 hours per night is a luxury requirement for many people. Occasionally, people lack the sense of the mind for one or two days, and may feel that the thinking is slow and the attention is not easy to concentrate. The consequences are more serious due to insufficient long-term sleep, and more scientific researches show that the physical and mental health is harmed. Following up to 25 years of follow-up in nearly 8000 british adults, researchers have analyzed that those who frequently sleep for less than 6 hours in the middle-aged years are at higher risk of developing alzheimer's disease and other dementia by the late years. Therefore, the knowledge of the public on the sleep is deepened, the sleep health is improved, and the sleep is very gentle.
At present, the treatment drugs for sleep disorder are the first choice, and the main drugs include barbiturates, benzodiazepines, non-benzodiazepine drugs, melatonin and some antidepressants. However, the drugs can generate the toxic and side effects of dependence, addiction, withdrawal symptoms, rebound phenomenon after drug withdrawal and the like after long-term administration, so the research and development of the safe and efficient sleep improving drugs are not slow. The Houttuynia cordata (Houttuynia cordita Thunb) is a perennial herb of Houttuynia of Saururaceae (Houttuynia), is rich in various nutritional ingredients such as protein, fat, calcium, phosphorus, iron, VC, VE and the like required by a human body, also contains various medicinal ingredients such as polysaccharide, flavone, volatile oil and the like, and is a wild plant with medicine and food functions. The polysaccharide substance is an organic compound with a broad-spectrum chemical structure and biological function in nature, widely exists in plants, animals and microorganisms, and has the effects of regulating immunity, resisting tumors, viruses, blood sugar and blood fat, resisting aging and the like. At present, the research on houttuynia cordata at home and abroad is mainly focused on the volatile oil component, and the sleep improvement effect of houttuynia cordata polysaccharide is not reported. Research on a subject group experiment finds that the houttuynia cordata polysaccharide extract has a good sleep improving effect, and has important theories and application values for research and development of medicaments and foods with the functions of widening the medicinal range of the houttuynia cordata and improving the sleep. At present, with the development of the big health industry, the self-care consciousness of people is strengthened, and the insomnia people in China are numerous, so that a wide consumer group is bound to be generated, and therefore, the research and development of the houttuynia cordata polysaccharide-related functional food for improving sleep can relieve the pain of some patients.
The cordate houttuynia is a traditional Chinese medicine in China, has dual purposes of medicine and food and is wide in clinical application. The herba Houttuyniae is whole herb of houttuynia cordata of houttuynia of Saururaceae, has pungent and cold nature, has effects of clearing heat and detoxicating, eliminating swelling and discharging pus, and inducing diuresis for treating stranguria, and can be used for treating swelling, carbuncle suppuration, phlegm heat cough, pyretic dysentery heat stranguria, carbuncle, suppurative sore, etc. The research proves that: the whole houttuynia cordata plant has a certain bacteriostatic action and comprises staphylococcus aureus, escherichia coli and saccharomycetes; the herba Houttuyniae volatile oil has effect in inhibiting influenza virus; intramuscular injection of the houttuynia cordata injection (whole plant aqueous extract) can obviously reduce the death rate of mice infected by influenza A virus H1N1, reduce the pulmonary index and inhibit the proliferation of viruses in the lung; herba Houttuyniae can also be used for resisting Herpes Simplex Virus (HSV) and SARS virus. Previous research results of the university of Compound Dan have proved that: houttuynia Cordata Polysaccharide (HCP) can obviously reduce lung injury induced by LPS, and in-vitro anti-hemolytic experiments also prove that the houttuynia cordata polysaccharide has obvious inhibition effect on excessive complement activation, which indicates that the HCP has obvious anti-inflammatory activity. With the help of influenza virus induced pneumonia model, it was confirmed that HCP can significantly treat virus-induced lung injury and also has protective effect on influenza virus-induced secondary intestinal injury.
The research on the houttuynia cordata is mostly seen in the research on lung diseases by comprehensively viewing domestic and foreign researches, and the medicine effect of the houttuynia cordata polysaccharide on treating the sleep disorder and the medicine research report thereof are not seen.
Disclosure of Invention
The invention aims to provide a new application of houttuynia polysaccharide in preparation of medicines or foods based on the current situation and the foundation of the prior art, and particularly relates to an application of houttuynia polysaccharide extract in preparation of medicines or foods for improving sleep.
The sleep improvement refers to the improvement of symptoms of difficulty in falling asleep and easy waking after sleeping or the treatment of primary insomnia and the like.
Further, the sleep improvement means the effect of enabling to fall asleep, rapidly fall asleep, reduce the sleep latency or prolong the sleep time.
The invention relates to a preparation method of houttuynia cordata polysaccharide extract, which is characterized in that houttuynia cordata medicinal materials are dried and crushed, soaked and extracted by pure water, ultrasonic treatment is carried out to remove residues, repeated extraction is carried out for a plurality of times, extracting solutions are combined and concentrated, 95% ethanol is added, standing and precipitating are carried out, upper layer suspended matters are centrifuged by a centrifugal machine, taken out, combined and precipitated, the precipitate is dissolved in acetone, fully washed and stood overnight, the precipitate is fully washed again by absolute ethyl alcohol, then standing is carried out, the precipitate is heated to a certain volume in a rotary evaporator after being completely precipitated, and vacuum drying is carried out to obtain crude houttuynia cordata polysaccharide.
Further, the drying of the houttuynia cordata medicinal material means that the houttuynia cordata is dried at 50-70 ℃, preferably 60 ℃.
Further, the pulverization means pulverization and sieving with 20-200 mesh, preferably 30-150 mesh, further preferably 40-100 mesh, further preferably 50-80 mesh, most preferably 60 mesh or 70 mesh.
Further, the weight volume ratio (g/ml) of the houttuynia cordata medicinal material powder to the pure water is 1:50-1, preferably 1:60-1:150, further preferably 1:70-1:100.
further, the soaking time by adding a certain volume of pure water is 12-36 hours, preferably 24 hours.
Further, the repeated extraction is performed 2 to 5 times, preferably 2 to 3 times, and more preferably 2 times.
The houttuynia polysaccharide extract is adopted to carry out an experiment on the influence of the houttuynia polysaccharide extract on the sleep of a mouse caused by a subthreshold dose of sodium pentobarbital, and the experiment result shows that the houttuynia polysaccharide extract obtained by separating and extracting from the traditional Chinese medicine houttuynia has a remarkable effect on improving the sleep through animal model experiments.
The invention also relates to a pharmaceutical composition for improving sleep, which is characterized by containing the houttuynia polysaccharide extract.
Further, the pharmaceutical composition also contains other pharmaceutically acceptable auxiliary materials.
The term "pharmaceutically acceptable excipient" as used herein includes any and all pharmaceutically acceptable carriers or diluents, solvents, dispersion media, coatings, surfactants, antioxidants, preservatives, isotonicity agents, absorption delaying agents, salts, preservatives, stabilizers, binders, excipients, disintegrants, lubricants, sweeteners, flavorants, dyes, and the like, or any combination thereof, as known to one of ordinary skill in the art. Any adjuvant known in the art may be used in the pharmaceutical composition or pharmaceutical preparation as long as it is compatible with the active ingredient.
Furthermore, the medicine composition also contains other components with the effect of improving sleep.
The invention also relates to a food for improving sleep, which is characterized by containing the houttuynia polysaccharide extract.
The houttuynia cordata polysaccharide extract for treating primary insomnia disclosed by the invention is low in effect taking dosage, good in effect and high in safety.
Drawings
FIG. 1 shows the effect of houttuynia cordata polysaccharide extract on the number of mice that sleep induced by sodium pentobarbital.
FIG. 2 is a graph showing the effect of houttuynia cordata polysaccharide extract on the sleep latency and sleep duration of sodium pentobarbital-induced mice.
FIG. 3 is a reaction diagram of measuring polysaccharide content by phenol-sulfuric acid method. (pentose and hexose dehydration reaction is shown in FIG. 3)
Detailed Description
Example 1 preparation method of houttuynia cordata polysaccharide
1. Materials and instruments
Houttuynia cordata thunb is purchased from Yixintang pharmacy of Kunming, yunnan province, and contains analytical reagents such as 95% ethanol, phenol, concentrated sulfuric acid, glucose, acetone and the like.
UV-5500PC type spectrophotometer (Shanghai chromatography Instrument Co., ltd.); RE-52A rotary evaporator (Shanghai Yangrong Biochemical Instrument factory); a medicinal grinder (large mechanical limited, forest, greens); model 101-2 electrothermal constant temperature air-blast drying oven (Beijing Kewei Instrument Co., ltd.).
2. Extraction of houttuynia cordata crude polysaccharide
Drying houttuynia cordata at 60 ℃, crushing and sieving the dried houttuynia cordata, accurately weighing 4.0g of powder, putting the powder into a 500mL beaker, adding a certain volume of pure water to soak for 24 hours, carrying out ultrasonic extraction for a certain time, filtering and removing residues, repeating the extraction for 2 times, combining filtrates, concentrating, adding 95% ethanol, standing and precipitating, using a centrifuge (4000 r/30 min) to remove upper-layer suspended matters, combining precipitates, dissolving the precipitates in acetone, fully washing and standing overnight, fully washing the precipitates again in absolute ethyl alcohol, standing, heating the precipitates to a certain volume in a rotary evaporator after the precipitates are completely precipitated, and carrying out vacuum drying to obtain crude polysaccharide of houttuynia cordata.
3. Polysaccharide content determination method
The determination principle is as follows: the method for measuring the content of polysaccharide by adopting a phenol-sulfuric acid method has the basic principle that sucrose is concentrated in H 2 SO 4 Under the action of the reaction (as shown in figure 3), furfural or hydroxymethyl furfural generated by intramolecular dehydration can be condensed with phenol to form an orange red compound, and has a maximum absorption peak at 490nm wavelength, so that the absorbance can be measured at the wavelength by a colorimetric method. The standard curve was made for this experiment with glucose. The method is simple, the reagent is cheap, the sensitivity is high, and the interference of the existence of protein is basically avoided during the experiment.
4. Determination of polysaccharides
Accurately weighing vacuum-dried herba Houttuyniae polysaccharide, dissolving in pure water, adding into 100mL volumetric flask, diluting to desired volume, and shaking to obtain polysaccharide stock solution. Accurately measuring 2mL of polysaccharide stock solution, placing the polysaccharide stock solution in a 25mL measuring flask, then adding 1mL of 4% phenol solution, shaking uniformly, rapidly adding 7mL of concentrated sulfuric acid, fixing the volume, placing the solution at room temperature for 30min, taking a corresponding reagent as a blank control, and measuring the absorbance at 490 nm.
5. The extraction rate is calculated by the formula that the extraction rate of polysaccharide is% = the mass (g) of polysaccharide in the extracting solution/the mass (g) of houttuynia cordata used multiplied by 100%.
Example 2 Effect of houttuynia cordata polysaccharide extract on sleep in mice at subthreshold dose of sodium pentobarbital
The male ICR mice are divided into houttuynia cordata polysaccharide high-low dose groups (2, 1 g/kg), blank groups and positive control groups, wherein each group comprises 12 mice. The mice in each group were administered by gavage for 14 days continuously, the blank group was administered with 0.5% carboxymethylcellulose solution, and the positive group was administered with diazepam (2 mg/kg). 30min after the last gastric lavage, the abdominal cavity of each mouse is injected with a subthreshold dose of pentobarbital sodium solution (30 mg/kg), and the number of mice in each group falling asleep within 15min is observed and recorded (the mice fall asleep when the righting reflex disappears for more than 1 min).
The influence of the houttuynia cordata polysaccharide extract on the sleep of the mice caused by the threshold dose of the sodium pentobarbital is divided into groups and administered in the same way, after each group of mice are continuously subjected to intragastric administration for 3 days and 30min after the last intragastric administration, the sodium pentobarbital solution (50 mg/kg) with the threshold dose is injected, and the sleep latency period and the sleep duration time of the mice are observed and recorded.
All data in the experiment were analyzed using SPSS 18.0 statistical software, including unpaired t-test, one-way ANOVA, chi-square test 2 ) Two-way ANOVA, and the like, as P<A difference of 0.05 was significant. Experimental data were plotted using GraphPad Prism5 plotting software using mean ± sem.
The experimental results are as follows:
(1) influence on the number of mice falling asleep (see FIG. 1)
** p<0.01， *** p<0.001; compared with the blank group, the high-dose group of the houttuynia cordata polysaccharide extract obviously increases the number of mice falling asleep (p<0.001 And have statistical differences.
(2) Effect on sleep latency and sleep duration in mice (see FIG. 2)
** p<0.01; *** p<0.001; compared with the blank group, the high and low dosage groups of houttuynia cordata polysaccharide extract can reduce the sleep latency and prolong the sleep time ** p<0.01， *** p<0.001 And have statistical differences.
Claims (9)
1. A preparation method of houttuynia cordata polysaccharide extract is characterized by comprising the steps of drying and crushing a houttuynia cordata medicinal material, soaking and extracting the houttuynia cordata medicinal material by pure water, removing residues through ultrasound, repeatedly extracting for a plurality of times, combining extracting solutions, concentrating, adding 95% ethanol, standing for precipitation, centrifuging upper-layer suspended matters by a centrifugal machine, taking out, combining precipitates, dissolving the precipitates in acetone, fully washing and standing overnight, fully washing the precipitates in absolute ethanol again, standing, heating the precipitates to a certain volume in a rotary evaporator after the precipitates are completely precipitated, and vacuum drying to obtain crude houttuynia cordata polysaccharide.
2. The method for preparing houttuynia cordata polysaccharide extract according to claim 1, wherein the weight-to-volume ratio (g/ml) of the houttuynia cordata medicinal material powder to pure water is 1:50-1, preferably 1:60-1:150, further preferably 1:70-1:100.
3. the method for preparing houttuynia cordata polysaccharide extract according to claim 1, wherein the drying of the houttuynia cordata is to dry the houttuynia cordata at 50-70 ℃, preferably 60 ℃.
4. The method for preparing houttuynia cordata polysaccharide extract according to claim 1, wherein the pulverization is performed by pulverizing and sieving the houttuynia cordata polysaccharide extract with a 20-200 mesh sieve, preferably a 30-150 mesh sieve, more preferably a 40-100 mesh sieve, still more preferably a 50-80 mesh sieve, and most preferably a 60 mesh sieve or a 70 mesh sieve.
5. The method for preparing houttuynia cordata polysaccharide extract according to claim 1, wherein the soaking time with a certain volume of pure water is 12-36 hours, preferably 24 hours.
6. The method for preparing houttuynia cordata polysaccharide extract according to claim 1, wherein the repeated extraction is performed 2 to 5 times, preferably 2 to 3 times, and more preferably 2 times.
7. A pharmaceutical composition for improving sleep, comprising an extract of houttuynia cordata polysaccharide, wherein the houttuynia cordata polysaccharide is prepared by the method according to any one of claims 1 to 6.
8. A food for improving sleep, characterized by comprising an extract of houttuynia cordata polysaccharide, which is prepared by the method according to any one of claims 1 to 6.
9. Use of a houttuynia cordata polysaccharide extract for the manufacture of a medicament or food product for improving sleep, wherein the houttuynia cordata polysaccharide is prepared by the method according to any one of claims 1 to 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211138881.4A CN115873142B (en) | 2022-09-19 | 2022-09-19 | Application of houttuynia cordata polysaccharide extract in preparation of medicines or foods |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211138881.4A CN115873142B (en) | 2022-09-19 | 2022-09-19 | Application of houttuynia cordata polysaccharide extract in preparation of medicines or foods |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115873142A true CN115873142A (en) | 2023-03-31 |
| CN115873142B CN115873142B (en) | 2024-05-14 |
Family
ID=85769907
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211138881.4A Active CN115873142B (en) | 2022-09-19 | 2022-09-19 | Application of houttuynia cordata polysaccharide extract in preparation of medicines or foods |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115873142B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107698689A (en) * | 2017-09-29 | 2018-02-16 | 贵州中科健生物医药有限公司 | A kind of method that polysaccharide is extracted from cordate houttuynia |
| CN114377180A (en) * | 2022-01-17 | 2022-04-22 | 宁国瑞 | Herbal smell removing bag, preparation method and use method |
-
2022
- 2022-09-19 CN CN202211138881.4A patent/CN115873142B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107698689A (en) * | 2017-09-29 | 2018-02-16 | 贵州中科健生物医药有限公司 | A kind of method that polysaccharide is extracted from cordate houttuynia |
| CN114377180A (en) * | 2022-01-17 | 2022-04-22 | 宁国瑞 | Herbal smell removing bag, preparation method and use method |
Non-Patent Citations (1)
| Title |
|---|
| 罗馨 等: "正交超声法提取鱼腥草多糖工艺研究", 化学工程师, vol. 26, no. 09, pages 9 - 12 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115873142B (en) | 2024-05-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1698750A (en) | Pharyngitis treating Chinese medicine | |
| JPS60214741A (en) | Hypoglycemic agent | |
| CN111097040A (en) | Antiviral lung-heat clearing peptide | |
| CN112057560A (en) | Ganoderma lucidum spore dendrobium nobile composition capable of enhancing immunity and preparation method and application thereof | |
| CA2955336C (en) | Method of extracting flavonoids and/or polyphenols from dried and powdered orange peels, compositions therefrom, and methods of treatment of diseases associated with chronic inflammation | |
| CN108210547B (en) | Preparation method of phyllanthus emblica leaf extract, preparation and anti-artemisia application thereof | |
| US9629385B2 (en) | Dietary composition containing cistanche deserticola polysaccharide with inhibitory effects on colon cancer | |
| CN101856415B (en) | Chinese herbal medicament oral liquid for treating chicken respiratory infectious disease | |
| CN115873142A (en) | Application of houttuynia polysaccharide extract in preparation of sleep improving medicines and health products | |
| KR101407889B1 (en) | Fraxinus rhynchophylla Hance extracts compositions for treating or preventing inflammatory diseases | |
| CN101732301B (en) | Application of chlorogenic acid in preparing medicines for preventing and curing senile dementia | |
| CN115645427A (en) | Application of baicalin in preparation of medicine for resisting pulmonary cell scorching | |
| CN106309758B (en) | Pharmaceutical composition for resisting gastrointestinal cancer | |
| CN107362271A (en) | A kind of Chinese medicine composition for treating breathing problem and preparation method thereof | |
| CN105816583B (en) | Compound medicine for treating cold and preparation method thereof | |
| CN1291730C (en) | Chinese medicine for preventing and treating SARS virus | |
| CN111000919A (en) | Application of ethanol extract of evodia lepta in preparation of medicine for treating sepsis | |
| CN103933376B (en) | Medicinal composition for treating chronic bronchitis | |
| CN103804392A (en) | Two terphenylzidioxazine derivatives and applications thereof | |
| CN102908577A (en) | Chinese and western compound medicine for treating respiratory system infection | |
| CN102247470B (en) | A kind of Chinese medicine composition preventing and treating infant upper respiratory tract infection | |
| CN103372055B (en) | Extraction process, the total polyphenols composition and use thereof of hypoglycemic activity composition total polyphenols in Cortex Cinnamomi | |
| CN113663024A (en) | Traditional Chinese medicine composition for treating viral myocarditis and preparation method thereof | |
| CN105561007A (en) | Traditional Chinese medicine preparation for treating infantile influenza and preparation method | |
| CN110731998A (en) | A Chinese medicinal composition for treating chronic pharyngitis |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |