CN115867676A - 细胞群的处理方法和细胞群中所含的基因的分析方法 - Google Patents

细胞群的处理方法和细胞群中所含的基因的分析方法 Download PDF

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CN115867676A
CN115867676A CN202180038544.5A CN202180038544A CN115867676A CN 115867676 A CN115867676 A CN 115867676A CN 202180038544 A CN202180038544 A CN 202180038544A CN 115867676 A CN115867676 A CN 115867676A
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cell
cells
sequence
population
droplets
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城口克之
金坚石
山本玲子
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RIKEN
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
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  • Engineering & Computer Science (AREA)
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  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202180038544.5A 2020-05-29 2021-05-28 细胞群的处理方法和细胞群中所含的基因的分析方法 Withdrawn CN115867676A (zh)

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JP2020094141 2020-05-29
JP2020-094141 2020-05-29
PCT/JP2021/020338 WO2021241721A1 (ja) 2020-05-29 2021-05-28 細胞集団の処理方法および細胞集団に含まれる遺伝子の分析方法

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US (1) US20230203600A1 (https=)
EP (1) EP4159873A4 (https=)
JP (1) JP7423101B2 (https=)
CN (1) CN115867676A (https=)
CA (1) CA3185619A1 (https=)
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Families Citing this family (1)

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CN114642684B (zh) * 2022-03-21 2024-07-26 北京航空航天大学 用于防治焦虑抑郁症的直肠真杆菌（Eubacterium rectale）

Citations (5)

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US20150154352A1 (en) * 2012-06-21 2015-06-04 Gigagen, Inc. System and Methods for Genetic Analysis of Mixed Cell Populations
US20170009274A1 (en) * 2015-02-04 2017-01-12 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
WO2018218222A1 (en) * 2017-05-26 2018-11-29 Goldfless Stephen Jacob High-throughput polynucleotide library sequencing and transcriptome analysis
US20190300968A1 (en) * 2018-03-27 2019-10-03 The Trustees Of Columbia University In The City Of New York Spatial Metagenomic Characterization of Microbial Biogeography
CN110462053A (zh) * 2016-12-21 2019-11-15 加利福尼亚大学董事会 使用基于水凝胶的液滴进行单细胞基因组测序

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WO2012083225A2 (en) * 2010-12-16 2012-06-21 Gigagen, Inc. System and methods for massively parallel analysis of nycleic acids in single cells
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
EP3110971B1 (en) * 2014-02-27 2019-05-08 The Broad Institute Inc. T cell balance gene expression and methods of use thereof
WO2015200541A1 (en) * 2014-06-24 2015-12-30 Bio-Rad Laboratories, Inc. Digital pcr barcoding
MX2018003534A (es) * 2015-09-25 2019-04-25 Abvitro Llc Proceso de alto rendimiento para identificacion de blanco de receptor de celula t de secuencias de receptor de celula t apareadas de manera natural.
JP7064439B2 (ja) * 2015-11-04 2022-05-10 アトレカ インコーポレイテッド 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット
CN109843435A (zh) * 2016-09-28 2019-06-04 通用自动化实验技术公司 用于细菌群落关系确定和其他高通量微生物学应用的高分辨率系统、试剂盒、设备和方法
EP4029939B1 (en) * 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
JP7160349B2 (ja) 2017-06-23 2022-10-25 国立研究開発法人理化学研究所 核酸をシークエンシングする方法および解析する方法
US11169152B2 (en) * 2017-09-15 2021-11-09 Battelle Memorial Institute Function-based probes for environmental microbiome analysis and methods of making and using the same

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150154352A1 (en) * 2012-06-21 2015-06-04 Gigagen, Inc. System and Methods for Genetic Analysis of Mixed Cell Populations
US20170009274A1 (en) * 2015-02-04 2017-01-12 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
CN107530654A (zh) * 2015-02-04 2018-01-02 加利福尼亚大学董事会 通过在离散实体中条形码化对核酸进行测序
CN110462053A (zh) * 2016-12-21 2019-11-15 加利福尼亚大学董事会 使用基于水凝胶的液滴进行单细胞基因组测序
WO2018218222A1 (en) * 2017-05-26 2018-11-29 Goldfless Stephen Jacob High-throughput polynucleotide library sequencing and transcriptome analysis
US20190300968A1 (en) * 2018-03-27 2019-10-03 The Trustees Of Columbia University In The City Of New York Spatial Metagenomic Characterization of Microbial Biogeography

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EP4159873A4 (en) 2024-07-17
CA3185619A1 (en) 2021-12-02
JP7423101B2 (ja) 2024-01-29
EP4159873A1 (en) 2023-04-05
JPWO2021241721A1 (https=) 2021-12-02
WO2021241721A1 (ja) 2021-12-02
US20230203600A1 (en) 2023-06-29

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