JP7423101B2 - 細胞集団の処理方法および細胞集団に含まれる遺伝子の分析方法 - Google Patents

細胞集団の処理方法および細胞集団に含まれる遺伝子の分析方法 Download PDF

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JP7423101B2
JP7423101B2 JP2022526658A JP2022526658A JP7423101B2 JP 7423101 B2 JP7423101 B2 JP 7423101B2 JP 2022526658 A JP2022526658 A JP 2022526658A JP 2022526658 A JP2022526658 A JP 2022526658A JP 7423101 B2 JP7423101 B2 JP 7423101B2
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JPWO2021241721A1 (https=
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克之 城口
ジャンシ ジン
れいこ 山本
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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JP2022526658A 2020-05-29 2021-05-28 細胞集団の処理方法および細胞集団に含まれる遺伝子の分析方法 Active JP7423101B2 (ja)

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JP2020094141 2020-05-29
JP2020094141 2020-05-29
PCT/JP2021/020338 WO2021241721A1 (ja) 2020-05-29 2021-05-28 細胞集団の処理方法および細胞集団に含まれる遺伝子の分析方法

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US (1) US20230203600A1 (https=)
EP (1) EP4159873A4 (https=)
JP (1) JP7423101B2 (https=)
CN (1) CN115867676A (https=)
CA (1) CA3185619A1 (https=)
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CN114642684B (zh) * 2022-03-21 2024-07-26 北京航空航天大学 用于防治焦虑抑郁症的直肠真杆菌（Eubacterium rectale）

Citations (6)

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WO2016126871A2 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
WO2018218222A1 (en) 2017-05-26 2018-11-29 Goldfless Stephen Jacob High-throughput polynucleotide library sequencing and transcriptome analysis
JP2018538006A (ja) 2015-11-04 2018-12-27 アトレカ インコーポレイテッド 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット
US20190002974A1 (en) 2010-12-16 2019-01-03 Gigagen, Inc. System and Methods for Massively Parallel Analysis of Nucleic Acids in Single Cells
US20190025299A1 (en) 2015-09-25 2019-01-24 Francois Vigneault High throughput process for t cell receptor target identification of natively-paired t cell receptor sequences
US20190300968A1 (en) 2018-03-27 2019-10-03 The Trustees Of Columbia University In The City Of New York Spatial Metagenomic Characterization of Microbial Biogeography

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WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
WO2013192570A1 (en) * 2012-06-21 2013-12-27 Gigagen, Inc. System and methods for genetic analysis of mixed cell populations
EP3110971B1 (en) * 2014-02-27 2019-05-08 The Broad Institute Inc. T cell balance gene expression and methods of use thereof
WO2015200541A1 (en) * 2014-06-24 2015-12-30 Bio-Rad Laboratories, Inc. Digital pcr barcoding
CN109843435A (zh) * 2016-09-28 2019-06-04 通用自动化实验技术公司 用于细菌群落关系确定和其他高通量微生物学应用的高分辨率系统、试剂盒、设备和方法
CA3047328A1 (en) * 2016-12-21 2018-06-28 The Regents Of The University Of California Single cell genomic sequencing using hydrogel based droplets
EP4029939B1 (en) * 2017-01-30 2023-06-28 10X Genomics, Inc. Methods and systems for droplet-based single cell barcoding
JP7160349B2 (ja) 2017-06-23 2022-10-25 国立研究開発法人理化学研究所 核酸をシークエンシングする方法および解析する方法
US11169152B2 (en) * 2017-09-15 2021-11-09 Battelle Memorial Institute Function-based probes for environmental microbiome analysis and methods of making and using the same

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190002974A1 (en) 2010-12-16 2019-01-03 Gigagen, Inc. System and Methods for Massively Parallel Analysis of Nucleic Acids in Single Cells
WO2016126871A2 (en) 2015-02-04 2016-08-11 The Regents Of The University Of California Sequencing of nucleic acids via barcoding in discrete entities
JP2018508198A (ja) 2015-02-04 2018-03-29 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア 別個の実体におけるバーコード付加による核酸のシーケンシング
US20190025299A1 (en) 2015-09-25 2019-01-24 Francois Vigneault High throughput process for t cell receptor target identification of natively-paired t cell receptor sequences
JP2018538006A (ja) 2015-11-04 2018-12-27 アトレカ インコーポレイテッド 単一細胞に関連する核酸の解析のための、核酸バーコードの組み合わせセット
WO2018218222A1 (en) 2017-05-26 2018-11-29 Goldfless Stephen Jacob High-throughput polynucleotide library sequencing and transcriptome analysis
US20190300968A1 (en) 2018-03-27 2019-10-03 The Trustees Of Columbia University In The City Of New York Spatial Metagenomic Characterization of Microbial Biogeography

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Nature Communications，2020年，11:866，pp.1-9，Published online: 13 February 2020

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EP4159873A4 (en) 2024-07-17
CA3185619A1 (en) 2021-12-02
EP4159873A1 (en) 2023-04-05
JPWO2021241721A1 (https=) 2021-12-02
CN115867676A (zh) 2023-03-28
WO2021241721A1 (ja) 2021-12-02
US20230203600A1 (en) 2023-06-29

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