CN115844939A - Artemisia annua extract with TRPV1 protein inhibiting effect - Google Patents
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Abstract
The invention provides application of a sweet wormwood herb extract in TRPV1 protein inhibition, wherein the sweet wormwood herb extract is prepared by a solvent extraction method. The invention also relates to application of the sweet wormwood herb extract in preparing products with TRPV1 protein inhibition effect, wherein the products are medicines, health-care foods or skin external preparations, and the sweet wormwood herb extract is prepared by adopting a solvent extraction method.
Description
Technical Field
The invention relates to the fields of natural medicinal chemistry and cosmetics, in particular to a sweet wormwood herb extract with a TRPV1 protein inhibition effect.
Background
Sensitive skin (sensitive skin) refers to a high-response state of skin under physiological or pathological conditions, mainly occurs on face, and can show subjective symptoms such as burning, stabbing pain, itching and tightness, accompanied or not accompanied by objective signs such as erythema, telangiectasia and desquamation. The proportion of sensitive skin is increasing along with factors such as environmental pollution, excessive cleanness and living pressure.
The skin barrier is the first line of defense of the skin, and an impaired or dysfunctional skin barrier may cause the skin to become more sensitive, thereby causing an inflammatory response. However, in the type of sensitive skin muscle, there are also cases where the skin barrier functions normally and no inflammatory reaction occurs, and there are also cases where nerve hyperreactivity occurs and receptors such as TRPs capable of sensing temperature, pain, etc. are expressed on the surface of keratinocytes.
TRPV1, as a heterogeneous receptor widely distributed in the peripheral nervous system, can be directly or indirectly activated by various ligands, and on one hand, the TRPV1 is transmitted to nerve centers through electric signals to generate symptoms such as tingling and itching, and on the other hand, calcium ion influx caused by TRPV1 activation can also cause neurogenic inflammation, and finally, the skin becomes reddish and erythema. Therefore, the inhibition of TRPV1 is also a key target for the evaluation of cosmetic raw materials with soothing efficacy.
At present, there are no commonly accepted TRPV1 antagonists, and only a few reports on the effects of some plant extracts in this respect, such as notopterygium root, cnidium fruit, green tangerine orange peel, gentian, etc. However, no report that the artemisia apiacea extract has the TRPV1 protein inhibitory effect has been found so far. For example, chinese patent application 202011402345.1 discloses an in vitro simulation of the cell line HaCaT with high TRPV1 expression, which is considered to be applicable to cytological methods for evaluating the discomfort of sensitive skin. Chinese patent application 202111561937.2 discloses a separation and purification method of Anoectochilus roxburghii glycoside and application thereof in soothing cosmetics, and reports that the extract can inhibit activation of TRPV1 on human embryonic kidney cells caused by capsaicin stimulation and reduce cell calcium ion influx caused by inflammatory mediators. Chinese patent application 202210359229.9 discloses a preparation method and application of a desalted purslane extract with TRPV1 antagonistic effect. However, neither of these literature reports nor patent applications relate to studies on the effect of artemisia apiacea extract in inhibiting TRPV 1.
The invention surprisingly discovers that the sweet wormwood herb extract has an inhibition effect on TRPV1 (transient receptor potential vanilloid1, TRPV 1) protein. In the research, the artemisia apiacea extract has excellent effect of inhibiting the content of TRPV1 protein, thereby preventing Ca 2+ Influx induced neurophatically sensitive skin discomfort. Furthermore, the sweet wormwood herb extract can be used as an efficacy additive for preparing medicines or skin external preparations to help improve the problems of skin sensitivity such as dry itching and stabbing pain and relieve the skin.
Disclosure of Invention
In one aspect, the invention provides an application of a sweet wormwood herb extract in TRPV1 protein inhibition, wherein the sweet wormwood herb extract is prepared by a solvent extraction method. In a preferred embodiment, the Artemisia annua extract is extracted with water as a solvent. In a preferred embodiment, the sweet wormwood herb extract is extracted by using ethanol with the volume concentration of 40-100% as a solvent. In a preferred embodiment, the extraction process of the artemisia apiacea extract is assisted by a method selected from the group consisting of: microwave, ultrasonic and reflux. In a preferred embodiment, the Artemisia annua extract is used at a concentration of 0.0001-20 wt%.
In another aspect, the present invention provides an application of an artemisia apiacea extract in the preparation of a product having a TRPV1 protein inhibitory effect, wherein the product is a pharmaceutical product, a health food or a skin external preparation, and the artemisia apiacea extract is prepared by a solvent extraction method. In a preferred embodiment, the Artemisia annua extract is extracted with water as a solvent. In a preferred embodiment, the sweet wormwood extract is extracted by using ethanol with the volume concentration of 40-100% as a solvent. In a preferred embodiment, the extraction process of the artemisia apiacea extract is assisted by a method selected from the group consisting of: microwave, ultrasonic and reflux. In a preferred embodiment, the Artemisia annua extract is used at a concentration of 0.0001-20 wt%.
In a preferred embodiment, the artemisia apiacea extract is used at a concentration of 0.1 to 5 vol% when the product is used as a skin external preparation. In a preferred embodiment, the Artemisia annua extract is used in a concentration of at least 0.01 wt.% when the product is used as a pharmaceutical product. In a preferred embodiment, the product is used as a health food with the use concentration of Artemisia annua extract at least equal to or greater than 1 wt%.
Brief description of the drawings
Fig. 1 shows the results of TRPV1 immunofluorescence with a magnification of 200.
Detailed Description
The invention relates to preparation and application of a sweet wormwood extract, which is found for the first time that the sweet wormwood extract has the function of inhibiting TRPV1, can be used as an effect additive for preparing medicines or skin external preparations to help improve the problems of skin sensitivity such as dry itching and tingling, and has the effect of relieving skin.
To provide a more concise description, some of the quantitative representations presented herein are not modified by the term "about". It is understood that, whether the term "about" is used explicitly or not, every quantity given herein is meant to refer to the actual given value, and it is also meant to refer to the approximation to such given value that would reasonably be inferred by one of ordinary skill in the art, including approximations due to experimental and/or measurement conditions for such given value.
To provide a more concise description, some quantitative expressions are recited herein as a range from about an X amount to about a Y amount. It should be understood that when a range is recited, the range is not limited to the upper and lower limits recited, but includes the entire range from about the X amount to about the Y amount or any amount therebetween.
Artemisia annua extract
The herba Artemisiae Annuae is dry aerial part of Artemisia annua L. Of Compositae, and is named as Artemisia annua, artemisia odorifera, artemisia annua, artemisia absinthium, artemisia ordosica, artemisia capillaris, artemisia annua, and Artemisia vulgaris. The Chinese pharmacopoeia records that the sweet wormwood herb clears summer heat, removes steam and prevents malaria. Can be used for treating fever due to summer-heat, fever due to yin deficiency, night fever with early coolness, hectic fever due to yin deficiency, malaria with cold-heat, and jaundice due to damp-heat. Modern pharmacological studies have focused on antimalarial effects on artemisinin-like drugs. In the search of the literature in the last 10 years, the artemisia apiacea and the skin are taken as key words, the effects mainly focus on the aspects of anti-inflammation, anti-allergy, immunoregulation, scar hyperplasia, antibiosis and the like, and the research on the aspects of acne and solar dermatitis inhibition and the like is also carried out, but the artemisia apiacea extract with the TRPV1 inhibition effect is not reported so far.
Under the experimental conditions (extraction process and efficacy research) in the invention, the effect of the artemisia apiacea extract on inhibiting the TRPV1 is proved, and the effect of the artemisia apiacea extract on skin soothing is proved.
The Artemisia apiacea extract is used as an effective active substance applied to medicines, health-care foods or skin external preparations, and has the effect of relieving the skin.
As the extraction method, a general solvent extraction method such as hot extraction, cold leaching, and percolation can be used, and a person skilled in the art can select an appropriate extraction method according to the actual situation.
In the case of solvent extraction of Artemisia annua, the extraction solvent should be selected to have the following properties: (1) the solubility is good, namely the solubility of the solvent to the required components is high, and the solubility to impurities is low, or vice versa; (2) inert, i.e. the solvent is not able to react chemically with the plant constituents, even if the reaction is reversible; (3) the operation is simple, the solvent can be conveniently separated from the extract after the extract is extracted, or the extract dissolved in the solvent can be directly used in the next step; (4) the method is economical and safe, namely, the method needs to consider economy, easy obtaining, low toxicity, convenient recovery and repeated use, certain safety and small environmental pollution when selecting the solvent.
The extraction can be carried out using an inert solvent and a reaction solvent. The inert solvent is a solvent which does not react with plant components, and can be water, ethanol, methanol, benzene, chloroform, ethyl acetate, acetone, etc. The reaction solvent is a solvent capable of increasing the solubility of acidic and alkaline components in plant in polar solvent, and can be diluted acid or diluted alkaline aqueous solution or alcoholic solution, such as hydrochloric acid, sulfuric acid, phosphoric acid, acetic acid, tartaric acid, sodium hydroxide, sodium carbonate, and sodium bicarbonate solution.
In some embodiments of the invention, the extraction is performed with water (e.g., deionized water). In some embodiments of the invention, the extraction is performed with ethanol. In some embodiments of the invention, ethanol may be used at a concentration of 40% to 100% by volume, preferably 50% to 98% ethanol, more preferably 70% to 95% ethanol.
The artemisia apiacea extract of the present invention can be prepared by microwave extraction. For example, water-assisted microwave methods may be employed for extraction.
The artemisia apiacea extract can be prepared by ultrasonic extraction. For example, ethanol (e.g., 70% ethanol by volume) can be used to assist the sonication process for extraction.
The artemisia apiacea extract of the present invention can be prepared by reflux extraction. For example, ethanol (e.g., 50% ethanol by volume) can be used to assist the reflux process for extraction.
In some embodiments, the artemisia apiacea extract is eluted through a macroporous resin. The macroporous resin used comprises: nonpolar, D-101 (national medicine reagent), XAD2 (Rohm and Haas), HP-20 (Mitsubishi chemical), HZ-802 (Waichang polymers Co.); weak polarity, AB-8 (Nankai Synthesis technology), DM-130 (Tianjin Hai Shi), XAD7 (Rohm and Haas); polarity, DM-301 (Tianjin Hai Shi), NKA-9 (Nankai Synthesis technologies), HZ-806 (Huachang polymers).
Medicine, health food or skin external preparation containing Artemisia annua extract
The sweet wormwood extract can be used as an effect additive to be applied to medicines, health-care foods or skin external preparations.
In some embodiments, the pharmaceutical product is selected from: tablets, capsules, emulsions, suspensions, powders, granules, solutions, and various pharmaceutical dosage forms known in the art. Different amounts are added according to different types of dosage forms.
The health food is also called functional food. It has functions of regulating human body function, but does not aim at treating diseases, and is suitable for specific people. In some embodiments, the health food may be in a powder form.
In some embodiments, the external skin agent is selected from: creams, lotions, gels, lotions, essences, masks, eye creams, aerosols (cleansing foams), sprays, body washes, and facial cleansers. Different amounts are added according to different types of formulations.
The external preparation for skin is a general concept of all ingredients generally used for the external part of skin, and may be, for example, a cosmetic composition. The cosmetic composition can be basic cosmetics, face makeup cosmetics, body makeup cosmetics, hair care cosmetics and the like, and the dosage form of the cosmetic composition is not particularly limited and can be reasonably selected according to different purposes. The cosmetic composition also contains different cosmetically acceptable media or matrix excipients according to different dosage forms and purposes.
The external preparation for skin contains a dermatologically acceptable carrier or vehicle (e.g., lotion, cream, ointment, cleanser, etc.). One of ordinary skill in the art will be able to select a carrier that will dissolve or disperse the components in the concentrations described above, based on common general knowledge in the art. When a carrier is used, the carrier should not cause inactivation of the artemisia annua extract and not cause any adverse effect on the skin when in use.
One of ordinary skill in the art will be able to select suitable carriers, including for example, water, alcohols, oils, and the like, based on the general knowledge and their ability to solubilize or disperse in the active ingredient at the concentrations most suitable for processing.
The skin external preparation of the present invention may be in the form of a topical application product, which can be externally applied to the skin, and can be prepared by those ordinary techniques well known in the art. The carrier may take any of a variety of practical forms such as a cream, dressing, gel, lotion, ointment or liquid comprising the applied and rinsed-off composition and incorporated into a carrier of material such as a dry or wet spread, hydrogel matrix, or adhesive (or non-adhesive) patch by methods well known in the art. Preferably, the carrier is a gel or a lotion that adds moisture, or a spread in dry or wet form.
Typical carriers include emulsions comprising water and/or an alcohol and an emollient such as hydrocarbon oils and waxes, silicone oils, hyaluronic acid, vegetable, animal or marine fats or oils, glyceride derivatives, fatty acids, or fatty acid esters or alcohols or alcohol ethers, lanolin and its derivatives, polyols or esters, wax esters, sterols, phospholipids and the like, and typically emulsifiers (nonionic, cationic or anionic), although some emollients inherently have emulsifying properties. In addition, these same components may be formulated into creams, gels, or solid sticks with varying proportions of their components and/or by incorporating thickeners such as gums or other forms of hydrocolloids.
The skin external preparation of the present invention may contain additional components commonly found in skin care compositions, such as emollients, skin conditioning agents, emulsifiers, preservatives, antioxidants, fragrances, chelating agents, and the like, as long as they are physically and chemically compatible with the other components of the skin external preparation and do not affect the effect of the artemisia annua extract of the present invention.
In some embodiments of the external preparation for skin of the present invention, one or more preservatives may be used. Suitable preservatives include p-hydroxyacetophenone, alkyl C1-C4-parabens and phenoxyethanol. Preservatives are used in amounts of about 0.5 to about 2 weight percent, preferably about 0.5 to 1 weight percent, based on the total weight of the composition.
In one example of the external preparation for skin of the present invention, one or more antioxidants may be used. Suitable antioxidants include Butylated Hydroxytoluene (BHT), ascorbyl palmitate (BHA), butylated hydroxyanisole, phenyl-alpha-naphthylamine, hydroquinone, gallopropyl, nordihydroguaiaretic acid, vitamin E or derivatives of vitamin E, vitamin C and its derivatives, calcium pantothenate, green tea extract and mixed polyphenols, and mixtures of the above. The antioxidant is used in an amount ranging from about 0.02 to 0.5 weight percent, more preferably from about 0.002 to 0.1 weight percent, based on the total weight of the composition.
In one example of the skin external preparation of the present invention, one or more emollients may be used, functioning as lubricants by their ability to remain on the surface of the skin or in the stratum corneum, to reduce flaking and improve the appearance of the skin. Typical emollients include fatty esters, fatty alcohols, mineral oils, polyether siloxane copolymers, and the like. Examples of suitable emollients include, without limitation, polypropylene glycol ("PPG") -15 stearyl ether, PPG-10 cetyl ether, steareth-10, oleth-8, PPG-4 lauryl ether, vitamin E acetate, lanolin, cetyl alcohol, cetearyl ethylhexanoate, cetearyl alcohol, glyceryl stearate, octyl hydroxystearate, dimethylpolysiloxane, and combinations thereof. Cetyl alcohol, cetearyl ethylhexanoate, cetostearyl alcohol, glyceryl stearate and combinations thereof are preferred. When used, the emollient is present in an amount ranging from about 0.1 to about 30 weight percent, preferably from about 1 to about 30 weight percent, based on the total weight of the composition.
In one example of the skin external preparation of the present invention, one or more moisturizers may be used. Humectants, also known as humectants, help to increase the effectiveness of the emollient, reduce flaking, stimulate the removal of compositional scales, and improve skin feel. Polyhydric alcohols may be used as humectants, including, but not limited to, glycerin, polyalkylene glycols, alkylene polyols and derivatives thereof, including butylene glycol, propylene glycol, dipropylene glycol, polyglycerol, polyethylene glycol and derivatives thereof, sorbitol, hydroxypropyl sorbitol, hexylene glycol, 1, 3-dibutylene glycol, 1,2, 6-hexanetriol, ethoxylated glycerin, propoxylated glycerin, and combinations thereof. When used, the humectant is present in an amount of about 0.1 to about 20 weight percent, preferably about 1 to about 15 weight percent, based on the total weight of the composition.
In one example of the external preparation for skin of the present invention, one or more emulsifiers may be used. The emulsifier may be used within the range of an effective stabilizing amount. Preferably, the emulsifier is used in an amount of about 1.0 to about 10.0 wt%, more preferably about 3.0 to about 6.0 wt%, based on the total weight of the composition. Any emulsifier that is compatible with the components of the composition may be used. Suitable emulsifiers include stearic acid, cetyl alcohol, glyceryl stearate, lecithin, stearyl alcohol, steareth-2, steareth-20, acrylates/C10-30 alkanol acrylate crosspolymers, and combinations thereof.
In one example of the external preparation for skin of the present invention, one or more pH adjusting agents may be used. The pH adjusting agent useful in the skin external preparation of the present invention includes tromethamine. When used, the pH adjusting agent is used in an amount of about 0.1 to about 2 weight percent, preferably about 0.1 to about 1 weight percent, based on the total weight of the composition.
In one embodiment of the present invention, the external preparation for skin comprises acrylic acid/C10-30 alkanol acrylate cross-linked polymer, glycerin, p-hydroxyacetophenone, glyceryl stearate and lecithin, cetostearyl alcohol, cetearyl ethyl hexanoate, tromethamine or a combination thereof.
In some embodiments of the present invention, the amount of the artemisia annua extract in the skin external preparation is 0.001-20% (w/w), preferably 0.01-20% (w/w), more preferably 0.01-10% (w/w), and most preferably 0.1-5% (w/w).
In one embodiment of the present invention, the amount of the artemisia apiacea extract in the skin external preparation is 0.1 to 5 vol%. In a preferred embodiment, the amount of the artemisia apiacea extract in the skin external preparation is 0.1 to 1% by volume.
Examples
The invention will be further illustrated by reference to the following specific examples. It should be noted that the examples are given solely for the purpose of illustration and are not to be construed as limitations on the scope of the invention, as many insubstantial modifications and variations of the invention may be made by those skilled in the art in light of the above teachings. Test methods in which specific conditions are not specified in the following examples are generally carried out under conventional conditions or under conditions recommended by the manufacturer. All percentages and parts are by weight unless otherwise indicated.
The sweet wormwood herbs used in the following examples are all from Jingwan drinking studios, bozhou, anhui. The centrifugation conditions included: shanghai Luxiang apparatus centrifuge, inc., centrifuging at 3500-5000 rpm at 3-20 deg.C for 5-20 min. The ultrasonic extraction conditions include: the ultrasonic time is 20-50 min, the ultrasonic time is 2-8 s, and the gap is 2-5 s. Ethanol and propylene glycol were purchased from national drug reagents and filter paper from Whatman company.
Macroporous resins employed in the examples include: nonpolar, D-101 (national medicine reagent), XAD2 (Rohm and Haas), HP-20 (Mitsubishi chemical), HZ-802 (Waichang polymers Co.); weak polarity, AB-8 (Nankai Synthesis technology), DM-130 (Tianjin Hai Shi), XAD7 (Rohm and Haas); polarity, DM-301 (Tianjin Haishuang), NKA-9 (Nankai Synthesis technologies), HZ-806 (Huachang polymers, inc.).
Example 1: preparation of Artemisia annua extract
Extracting herba Artemisiae Annuae 50g with deionized water 15 times for 2 times, each time for 1 hr, filtering with screen (20 mesh, shanghai Yichang instrument screen), concentrating the filtrate to 3 times of crude drug amount, adding 95% ethanol, precipitating with ethanol, filtering with filter paper, concentrating, recovering ethanol, and making into solution with solid content of 1.5%.
Example 2: preparation of sweet wormwood extract
Extracting herba Artemisiae Annuae 75g with deionized water 25 times by microwave for 1 time, microwave extracting for 45min at 35 deg.C and power of 0.60kw, centrifuging (centrifuging at 5000rpm for 10 min), filtering, concentrating the filtrate to 2 times of crude drug amount, adding 95% ethanol, precipitating with ethanol, centrifuging (centrifuging at 5000rpm for 10 min), filtering, concentrating, recovering ethanol, loading into large pores (D-101, chinese medicinal reagent), eluting, recovering ethanol, and preparing into 5.5% solid solution.
Example 3: preparation of sweet wormwood extract
Extracting herba Artemisiae Annuae 125g with 70% ethanol 20 times under ultrasonic for 2 times, ultrasonic treating for 40min each time (ultrasonic at room temperature, ultrasonic treating for 6s, and gap 2 s), centrifuging (centrifuging at 5000rpm for 10 min), filtering, concentrating the filtrate, recovering ethanol, and making into solution with solid content of 2.5%.
Example 4: preparation of sweet wormwood extract
Extracting herba Artemisiae Annuae 200g with 10 times deionized water for 2 times, filtering with screen (20 mesh, shanghai Yichang instrument screen factory), concentrating the filtrate to 1 time crude drug amount, adding a certain amount of 95% ethanol for precipitating, filtering with filter paper, concentrating and recovering ethanol, and concentrating with 50nm ceramic membrane (Xiamen Fumei technology) to obtain solution containing 4.5% of solid.
Example 5: preparation of sweet wormwood extract
Extracting herba Artemisiae Annuae 250g with 6 times of 50% ethanol under reflux at 60 deg.C for 1 time, extracting for 1.5 hr, centrifuging (5000 rpm for 10 min), filtering, adding deionized water and glycerol into the filtrate, wherein glycerol is 50%, and preparing into 3.5% solution with deionized water.
Example 6: keratinocyte-based protein content detection of capsaicin receptor (TRPV 1)
1. The testing principle is as follows:
the soothing efficacy of the test sample is evaluated by measuring the protein content of capsaicin receptor (TRPV 1) based on keratinocytes. After the test substance acts, the TRPV1 is inhibited, which can show that the test substance achieves the effect of relieving by inhibiting the TRPV 1.
2. Testing materials:
keratinocytes Ep21072702 (Guangdong Boxi), KC2500 (Guangdong Boxi), PBS (Solibao), MTT (Sigma), DMSO (Sigma), paraformaldehyde (national medicine), TRPV1 antibody (Abcam).
CO2 incubator (Thermo, 150I), clean bench (SW-CJ-1F, antai Suzhou), inverted microscope (Olympus, CKX 41), fluorescence microscope (Leica, DM 2500).
3. And (3) testing a system:
4. the test method comprises the following steps:
1) Cell inoculation: by 1.8X 10 5 The density of the cells/well was inoculated with keratinocytes in 6-well plates, incubator (37 ℃, 5% CO) 2 ) And incubated overnight.
2) Administration: according to the table test system, when the cell plating rate in the 6-hole plate reaches 40% -50%, the medicine is administered in groups, the medicine dose in each hole is 2mL, and each group is provided with 3 multiple holes. After completion of administration, the 6-well plate was placed in an incubator (37 ℃, 5% 2 ) InIncubation was carried out for 24h.
3) TRPV1 detection: fixing with 4% paraformaldehyde for 30min, performing immunofluorescence detection of capsaicin receptor (TRPV 1), taking pictures under microscope, collecting pictures, and analyzing.
4) And (3) statistical analysis of results: the results are expressed as Mean ± SD using GraphPad Prism mapping. The comparison between groups was performed by statistical analysis of t-test. P <0.05 was considered to have significant differences, and P <0.01 was considered to have very significant differences.
5. Test results
Based on the test protocol, TRPV1 detection was performed according to the immunofluorescence protocol, and the detection results are shown in fig. 1. FIG. 1 shows the result of TRPV1 immunofluorescence, wherein green fluorescence represents the content of the target protein "TRPV1", and the higher the fluorescence intensity, the higher the protein content.
The following table shows the results of TRPV1 protein content detection:
TABLE 1
Remarking: when the statistical analysis is carried out by a t-test method, compared with the BC group, the significance is represented by #, the P-value is less than 0.05 and is represented by #, and the P-value is less than 0.01 and is represented by #; significance was expressed as x, P-value <0.05 as x, P-value <0.01 as x, compared to NC group.
The experimental result shows that compared with the BC group, the TRPV1 protein content of the NC group is obviously increased, which indicates that the stimulation condition of the test is effective. Compared with the NC group, the content of TRPV1 protein of the artemisia apiacea extract in the concentrations of 0.6% (v/v), 0.3% (v/v) and 0.1% (v/v) is greatly reduced (p < 0.01) in example 4, which shows that the artemisia apiacea extract has a very significant inhibition effect on the TRPV1 in 3 concentrations.
The sweet wormwood herb extract can be used as an intermediate raw material or an efficacy additive for preparing medicines, health-care foods or skin external preparations, and the skin external preparations are preferably cosmetic compositions, including but not limited to products in dosage forms of face cream, milky lotion, gel, cosmetic water, essence, facial mask, eye cream, aerosol (cleaning foam), spray, shower gel, facial cleanser and the like. Examples 1-5 the weight percentage of the artemisia apiacea extract in the skin external preparation may be 0.0001-20% (w/w), preferably 0.001-10% (w/w), more preferably 0.001-5% (w/w), and most preferably 0.01-5% (w/w).
The following are specific examples of the application of the Artemisia annua extract obtained in examples 1-5 to skin external preparations, and the formulation and preparation method of these preparations. The specific application examples are as follows:
application example 1: preparation of capsules
Taking the solid solution of the sweet wormwood herb extract prepared in the example 1, concentrating the solid solution into thick paste, adding maltodextrin, uniformly mixing, granulating, and encapsulating, wherein each capsule contains 0.2g of the solid solution, and preparing into 1000 capsules.
Application example 2: preparation of Chinese medicinal preparation
The solid solution of the artemisia apiacea extract prepared in the example 2 is processed by a membrane (200 nm ceramic membrane) at room temperature, the molecular weight cut-off is more than 30000, and the solution passing through the membrane (the molecular weight is less than 30000) is stored for standby. Filtering the solution with molecular weight less than 30000 with nanofiltration membrane at room temperature, and retaining the concentrated solution with molecular weight more than 300 for use. And (3) carrying out sterile freeze drying on the concentrated solution with the molecular weight of more than 300 to prepare freeze-dried powder. The freeze-dried powder is prepared into capsules and powder injections to obtain a target product, namely a traditional Chinese medicine preparation prepared from the sweet wormwood herb extract.
Application example 3: preparation of face cream
Application example 4: preparation of the emulsion
Application example 5: preparation of jelly
Application example 6: preparation of astringent
Application example 7: preparation of essence
Example 8 should be mentioned: preparation of facial mask
Application example 9: preparation of eye cream
Application example 10: preparation of an aerosol (cleaning foam)
Application example 11: preparation of the spray
Application example 12: preparation of shower gel
Application example 13: preparation of facial cleanser
Claims (10)
1. The application of the sweet wormwood herb extract in the inhibition of TRPV1 protein is disclosed, wherein the sweet wormwood herb extract is prepared by adopting a solvent extraction method.
2. The use as claimed in claim 1, wherein the Artemisia annua extract is extracted with water as solvent.
3. The use as claimed in claim 1, wherein the Artemisia annua extract is extracted with ethanol with a volume concentration of 40-100% as a solvent.
4. The use as claimed in claim 1, wherein the extraction process of the artemisia annua extract is assisted by a method selected from the group consisting of: microwave, ultrasonic and reflux.
5. The use as claimed in claim 1, wherein the artemisia apiacea extract is used at a concentration of 0.0001-20 wt%.
6. The application of the sweet wormwood herb extract in preparing products with TRPV1 protein inhibition effect, wherein the products are medicines, health-care foods or skin external preparations, and the sweet wormwood herb extract is prepared by adopting a solvent extraction method.
7. The use as claimed in claim 6, wherein the Artemisia annua extract is extracted with water as solvent.
8. The use as claimed in claim 6, wherein the Artemisia annua extract is extracted with ethanol with a volume concentration of 40-100% as solvent.
9. The use as claimed in claim 6, wherein the extraction process of Artemisia annua extract is assisted by a method selected from the group consisting of: microwave, ultrasonic and reflux.
10. The use as claimed in claim 6, wherein the Artemisia annua extract is used at a concentration of 0.0001-20 wt%.
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| CN109939105A (en) * | 2017-12-20 | 2019-06-28 | 隐纱化妆品有限公司 | The purposes of qinghaosu and its derivative |
| CN111925979A (en) * | 2020-08-14 | 2020-11-13 | 贵州省人民医院 | Construction method of animal and cell model for improving acne inflammation of roses by artemisinin |
| CN113855718A (en) * | 2021-09-30 | 2021-12-31 | 上海家化联合股份有限公司 | Artemisia apiacea extract and application thereof |
| CN115607493A (en) * | 2022-10-19 | 2023-01-17 | 广州伽能生物科技有限公司 | Itching-relieving and soothing composition and preparation method thereof |
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2022
- 2022-12-26 CN CN202211676162.8A patent/CN115844939A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109939105A (en) * | 2017-12-20 | 2019-06-28 | 隐纱化妆品有限公司 | The purposes of qinghaosu and its derivative |
| CN108478489A (en) * | 2018-05-18 | 2018-09-04 | 上海家化联合股份有限公司 | The moisturizing purposes of Artemisinin and the dermal compositions containing Artemisinin |
| CN111925979A (en) * | 2020-08-14 | 2020-11-13 | 贵州省人民医院 | Construction method of animal and cell model for improving acne inflammation of roses by artemisinin |
| CN113855718A (en) * | 2021-09-30 | 2021-12-31 | 上海家化联合股份有限公司 | Artemisia apiacea extract and application thereof |
| CN115607493A (en) * | 2022-10-19 | 2023-01-17 | 广州伽能生物科技有限公司 | Itching-relieving and soothing composition and preparation method thereof |
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