CN115820706B - 半乳糖-1-磷酸尿苷酰基转移酶突变体及其在制备l-赖氨酸中的应用 - Google Patents

半乳糖-1-磷酸尿苷酰基转移酶突变体及其在制备l-赖氨酸中的应用

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Publication number
CN115820706B
CN115820706B CN202211706015.0A CN202211706015A CN115820706B CN 115820706 B CN115820706 B CN 115820706B CN 202211706015 A CN202211706015 A CN 202211706015A CN 115820706 B CN115820706 B CN 115820706B
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CN
China
Prior art keywords
recombinant
lysine
biological cell
ncgl2002
seq
Prior art date
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Active
Application number
CN202211706015.0A
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English (en)
Chinese (zh)
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CN115820706A (zh
Inventor
周晓群
孟刚
魏爱英
赵春光
马风勇
张英
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ningxia Eppen Biotech Co Ltd
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Ningxia Eppen Biotech Co Ltd
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Application filed by Ningxia Eppen Biotech Co Ltd filed Critical Ningxia Eppen Biotech Co Ltd
Priority to CN202211706015.0A priority Critical patent/CN115820706B/zh
Publication of CN115820706A publication Critical patent/CN115820706A/zh
Priority to EP23908881.8A priority patent/EP4644555A1/en
Priority to PCT/CN2023/084971 priority patent/WO2024138928A1/zh
Priority to JP2025538710A priority patent/JP2026501022A/ja
Application granted granted Critical
Publication of CN115820706B publication Critical patent/CN115820706B/zh
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/0701—UTP--hexose-1-phosphate uridylyltransferase (2.7.7.10)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/15—Corynebacterium

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202211706015.0A 2022-12-29 2022-12-29 半乳糖-1-磷酸尿苷酰基转移酶突变体及其在制备l-赖氨酸中的应用 Active CN115820706B (zh)

Priority Applications (4)

Application Number Priority Date Filing Date Title
CN202211706015.0A CN115820706B (zh) 2022-12-29 2022-12-29 半乳糖-1-磷酸尿苷酰基转移酶突变体及其在制备l-赖氨酸中的应用
EP23908881.8A EP4644555A1 (en) 2022-12-29 2023-03-30 Galactose-1-phosphate uridyltransferase mutant and use thereof in preparation of l-lysine
PCT/CN2023/084971 WO2024138928A1 (zh) 2022-12-29 2023-03-30 半乳糖-1-磷酸尿苷酰基转移酶突变体及其在制备l-赖氨酸中的应用
JP2025538710A JP2026501022A (ja) 2022-12-29 2023-03-30 ガラクトース－１－リン酸ウリジルトランスフェラーゼ変異体及びそのｌ－リジンの製造における使用

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202211706015.0A CN115820706B (zh) 2022-12-29 2022-12-29 半乳糖-1-磷酸尿苷酰基转移酶突变体及其在制备l-赖氨酸中的应用

Publications (2)

Publication Number Publication Date
CN115820706A CN115820706A (zh) 2023-03-21
CN115820706B true CN115820706B (zh) 2025-07-29

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Country Status (4)

Country Link
EP (1) EP4644555A1 (https=)
JP (1) JP2026501022A (https=)
CN (1) CN115820706B (https=)
WO (1) WO2024138928A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115820706B (zh) * 2022-12-29 2025-07-29 宁夏伊品生物科技股份有限公司 半乳糖-1-磷酸尿苷酰基转移酶突变体及其在制备l-赖氨酸中的应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110869504A (zh) * 2017-06-07 2020-03-06 齐默尔根公司 来自谷氨酸棒状杆菌的启动子及其在调节辅助基因表达中的用途
CN114835783A (zh) * 2022-06-01 2022-08-02 宁夏伊品生物科技股份有限公司 NCgl2747基因突变体及其在制备L-赖氨酸中的应用

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100789271B1 (ko) * 2005-11-30 2008-01-02 씨제이 주식회사 Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용하여 ｌ-라이신을 생산하는 방법
KR100838038B1 (ko) * 2006-12-29 2008-06-12 씨제이제일제당 (주) Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용한 ｌ-라이신 생산 방법
KR100838035B1 (ko) * 2006-12-29 2008-06-12 씨제이제일제당 (주) Ｌ-라이신 생산능이 향상된 코리네박테리움 속 미생물 및그를 이용한 ｌ-라이신 생산 방법
CN105950524A (zh) * 2016-04-27 2016-09-21 齐鲁工业大学 一种高产l-赖氨酸的谷氨酸棒状杆菌工程菌的构建方法
CN114605509B (zh) * 2022-03-14 2024-06-04 宁夏伊品生物科技股份有限公司 Yh66_01475蛋白及其编码基因在调控细菌精氨酸产量中的应用
CN115820706B (zh) * 2022-12-29 2025-07-29 宁夏伊品生物科技股份有限公司 半乳糖-1-磷酸尿苷酰基转移酶突变体及其在制备l-赖氨酸中的应用

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110869504A (zh) * 2017-06-07 2020-03-06 齐默尔根公司 来自谷氨酸棒状杆菌的启动子及其在调节辅助基因表达中的用途
CN114835783A (zh) * 2022-06-01 2022-08-02 宁夏伊品生物科技股份有限公司 NCgl2747基因突变体及其在制备L-赖氨酸中的应用

Also Published As

Publication number Publication date
WO2024138928A1 (zh) 2024-07-04
CN115820706A (zh) 2023-03-21
JP2026501022A (ja) 2026-01-13
EP4644555A1 (en) 2025-11-05

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