CN115814011B

CN115814011B - Heart-clearing, sleep-aiding and intelligence-improving medicinal pillow and preparation method thereof

Info

Publication number: CN115814011B
Application number: CN202211605255.1A
Authority: CN
Inventors: 杨桂芹; 李烁; 王红柳; 朱华; 耿宁果; 杨梅; 王晓静; 孙莉琼; 唐晓清; 金巾; 吉萍
Original assignee: Nanjing Agricultural University
Current assignee: Nanjing Agricultural University
Priority date: 2022-12-14
Filing date: 2022-12-14
Publication date: 2023-11-03
Anticipated expiration: 2042-12-14
Also published as: CN115814011A

Abstract

The invention discloses a heart-fire clearing and sleep-aiding and intelligence-improving medicinal pillow and a preparation method thereof, wherein a traditional Chinese medicinal composition in the medicinal pillow comprises poria with hostwood, polygala tenuifolia, grassleaf sweetflag rhizome, dragon bone, oyster shell, mother-of-pearl, stir-fried wild jujube seed, tuber fleeceflower stem, glossy privet fruit, eclipta, chinese magnoliavine fruit, flos albiziae, rose, coptis chinensis, cinnamon, rush, nutgrass galingale rhizome, gardenia, costustoot, frankincense, biota oriental arborvitae and mulberry leaf. The medicine pillow has the functions of clearing away heart fire, soothing the nerves, improving sleep quality, strengthening brain and improving intelligence; meanwhile, the medicine has the characteristics of safety, reliability, low use cost, long medicine effect period and convenience in carrying, is suitable for people of all ages, and has wide application prospect.

Description

Heart-clearing, sleep-aiding and intelligence-improving medicinal pillow and preparation method thereof

Technical Field

The invention relates to a medicine pillow and a preparation method thereof, in particular to a heart-clearing sleep-aiding and intelligence-improving medicine pillow and a preparation method thereof.

Background

The health status of a person can be divided into three states, healthy, sub-healthy and disease. According to a global investigation by the World Health Organization (WHO), people in a healthy state account for 5% of the general population, people in a diseased state account for 20% of the general population, and less than 25% of the total population, more than 75% of the people are in a transitional state between healthy and diseased, also referred to as a "sub-healthy state". Sub-health is reversible, and if it is in sub-health state for a long period, it can develop into sub-clinical state and further into disease state.

Insomnia is the most common symptom of sub-health status, which is a functional disorder caused by imbalance of brain function balance, and is a status that is often difficult to fall asleep, easy to wake after sleeping, or wake early, caused by disorder of sleep rhythm caused by reasons related to modern life style. Questionnaire analysis, wherein most people are insomniac with symptoms such as dysphoria, dreaminess, drowsiness, lazy after waking, memory decline and the like, and the biological clock is disturbed due to irregular long-term work and rest, and the circadian rhythm is regulated and controlled to be unbalanced; the study and research pressure is great, and the thinking is that liver qi cannot reach smoothly, and the gallbladder fire disturbs the heart and the fire disturbs the mind.

At present, the modern medicine has the treatment principle of 'taking on demand' and 'intermittent small dosage' of hypnotic sedative. However, the study shows that the sedative hypnotic drug used for a long time is easy to generate various adverse reactions such as dependence, tolerance, withdrawal symptoms, rebound effect and the like, thereby becoming a cause for inducing insomnia. The modern medicine pillow has the advantages of multiple functions, complex using steps, single medicine flavor, low universality and the like, and needs to be further improved.

Disclosure of Invention

The invention aims to: the invention aims to provide a heart-fire clearing, sleep-aiding and intelligence-improving medicinal pillow which is beneficial to improving sleep quality; the invention further aims at providing a preparation method of the heart-clearing, sleep-aiding and intelligence-improving medicinal pillow.

The technical scheme is as follows: the invention relates to a heart-clearing, sleep-aiding and intelligence-improving medicinal pillow, which comprises a pillowcase and a pillow core, wherein the pillow core is detachably connected with the pillowcase, and the pillow core contains a medicinal bag of traditional Chinese medicines. The traditional Chinese medicinal materials in the traditional Chinese medicinal bag comprise the following components in mass:

25-32 parts of poria with hostwood, 5-12 parts of polygala tenuifolia, 5-12 parts of rhizoma acori graminei, 25-32 parts of polygonum multiflorum, 25-32 parts of dragon bone, 25-32 parts of mother-of-pearl, 25-32 parts of oyster, 25-32 parts of spina date seed, 15-22 parts of glossy privet fruit, 15-22 parts of eclipta alba, 15-22 parts of schisandra chinensis, 10-18 parts of albizia flower, 10-18 parts of rose, 5-12 parts of coptis chinensis, 1-8 parts of cinnamon, 5-12 parts of rush, 5-12 parts of rhizoma cyperi, 5-12 parts of gardenia, 5-12 parts of elecampane, 1-8 parts of frankincense, 10-18 parts of biota orientalis and 10-18 parts of mulberry leaf.

Wherein, the main drugs mainly have the following properties and effects:

poria with pine needle: sweet, light and flat, enter heart and spleen meridians. Calm heart, tranquilize mind and induce diuresis. Is used for treating palpitation due to heart deficiency, insomnia, amnesia, and convulsion.

Radix polygalae: bitter and pungent, warm. It enters heart, kidney and lung meridians. Tranquillization and intelligence development, transportation of heart and kidney, phlegm elimination and detumescence. Can be used for treating insomnia, dreaminess, amnesia, palpitation, absentmindedness, expectoration, pyocutaneous disease, swelling and toxin, and breast swelling and pain caused by heart-kidney disharmony.

Rhizoma Acori Graminei: pungent and bitter, warm. It enters heart and stomach meridians. Inducing resuscitation, eliminating phlegm, refreshing mind, improving intelligence, eliminating dampness and stimulating appetite. Can be used for treating unconsciousness, epilepsy, amnesia, insomnia, tinnitus, deafness, gastric distention, hunger, and dysentery.

Caulis Polygoni Multiflori: sweet and flat. It enters heart and liver meridians. Nourishing blood, tranquillizing, dispelling wind and dredging collaterals. Can be used for treating insomnia, dreaminess, blood deficiency, body pain, rheumatalgia, and skin pruritus.

Keel: sweet, astringent and flat, it enters heart, liver and kidney meridians. Calm and calm the mind, calm the liver and calm the yang, astringe and arrest the flow of astringents. Is mainly used for treating restlessness of heart-mind, palpitation, insomnia, convulsive epilepsy, mania, dizziness due to liver yang, etc.

Mother of pearl: salty and cold. Enter liver and heart meridians. Pacify liver and subdue yang, tranquilize and calm convulsion, improve eyesight and remove nebula. Can be used for treating headache, dizziness, palpitation, insomnia, conjunctival congestion, nebula, and blurred vision.

Oyster: salty and slightly cold. It enters liver, gallbladder and kidney meridians. The medicine has the functions of relieving the weight, tranquilizing the mind, suppressing yang, tonifying yin, softening hardness and dissipating nodulation. Can be used for treating palpitation, insomnia, dizziness, tinnitus, scrofula, phlegm nodule, and abdominal mass. Calcined oyster shell, concha Ostreae, has astringents to induce astringency, and is effective in relieving hyperacidity and pain. Can be used for treating spontaneous perspiration, night sweat, spermatorrhea, metrorrhagia, leukorrhagia, and stomach ache.

Semen Ziziphi Spinosae: sweet and flat. Enter heart and liver meridian. Nourish the heart and tranquilize the mind, and arrest sweating. Can be used for treating insomnia, palpitation, spontaneous perspiration, night sweat, etc.

Fructus ligustri lucidi: sweet, bitter and cool. It enters liver and kidney meridians. Nourishing liver and kidney, improving eyesight and blackening hair. Can be used for treating deficiency of liver-yin and kidney-yin, dizziness, tinnitus, soreness of waist and knees, premature gray hair, dim eyesight, internal heat, diabetes, hectic fever, and bone steaming.

Ecliptae herba: sweet, sour and cold. It enters kidney and liver meridians. Nourishing liver and kidney, cooling blood and stopping bleeding. Can be used for treating deficiency of liver-yin and kidney-yin, odontoseisis, premature gray hair, dizziness, tinnitus, soreness of waist and knees, hematemesis due to yin deficiency and blood heat, epistaxis, hematuria, bloody dysentery, metrorrhagia, hemorrhage due to traumatic hemorrhage.

Chinese magnoliavine fruit: acid, sweet, warm. It enters lung, heart and kidney meridians. Astringe and astringe, replenish qi and promote the production of body fluid, tonify kidney and calm heart. Can be used for treating chronic cough, deficiency asthma, nocturnal emission, enuresis, frequent urination, chronic diarrhea, spontaneous perspiration, night sweat, body fluid consumption, thirst, internal heat, diabetes, palpitation, and insomnia.

Albizia flower: sweet and flat. It enters heart and liver meridians. Resolving depression and tranquillizing. Can be used for treating mental disorder, depression and insomnia.

Rose: sweet, slightly bitter and warm. Enter liver and spleen meridians. Promoting qi circulation, resolving stagnation, regulating blood, and relieving pain. Can be used for treating pain of liver and stomach, anorexia, nausea, menoxenia, traumatic injury.

Coptis root: bitter and cold. It enters heart, spleen, stomach, liver, gall bladder and large intestine meridians. Clear heat and dry dampness, purge fire and detoxify. Can be used for treating damp-heat distention and fullness, emesis, acid regurgitation, dysentery, jaundice, high fever unconsciousness, excessive heart fire, vexation, insomnia, palpitation, etc.

Cinnamon: pungent, sweet and hot. It enters kidney, spleen, heart and liver meridians. Tonifying fire and invigorating yang, inducing fire to return to the original form, dispelling cold and relieving pain, and warming and activating meridians. Can be used for treating sexual impotence, cold womb, cold pain of waist and knee, asthma due to kidney deficiency, deficient yang floating up, giddiness, conjunctival congestion, cold pain of heart and abdomen, vomiting and diarrhea due to deficiency cold, cold hernia, abdominal pain, dysmenorrhea, and amenorrhea.

And (3) rhizoma cyperi: pungent, slightly bitter, slightly sweet, and flat. It enters liver, spleen and triple energizer meridians. Liver soothing, qi stagnation relieving, qi regulating, middle energizer regulating, menstruation regulating and pain relieving. Can be used for treating liver depression, qi stagnation, chest and hypochondrium distending pain, hernia pain, breast distending pain, spleen and stomach qi stagnation, epigastric fullness and distention pain, menoxenia, amenorrhea and dysmenorrhea.

Gardenia flower: bitter and cold. It enters heart, lung and triple energizer meridians. Purging pathogenic fire, relieving restlessness, clearing heat, promoting diuresis, cooling blood, and removing toxic substances; external use detumescence and pain relieving. Can be used for treating febrile vexation, jaundice due to damp-heat, stranguria, hematemesis, conjunctival congestion, swelling and pain, and pyocutaneous disease due to pathogenic fire; it is indicated for sprain, contusion and pain.

Radix aucklandiae: pungent and bitter, warm. It enters spleen, stomach, large intestine, triple energizer and gallbladder meridians. Promoting qi circulation, relieving pain, invigorating spleen, and resolving food stagnation. Can be used for treating chest and hypochondrium pain, abdominal distention, diarrhea, dyspepsia, anorexia. Stewing the banksia rose with the intestine to check diarrhea. Can be used for treating diarrhea and abdominal pain.

Mastic gum: pungent and bitter, warm. It enters heart, liver and spleen meridians. Promoting blood circulation, relieving pain, detumescence and promoting granulation. Can be used for treating chest pain, pain in stomach, dysmenorrhea, amenorrhea, puerperal stagnation, abdominal mass, abdominal pain, rheumatalgia, spasm of tendons and vessels, traumatic injury, carbuncle, swelling and sore.

Biota orientalis leaf: bitter, astringent and cold. It enters lung, liver and spleen meridians. Cool blood, stop bleeding, resolve phlegm, stop cough, promote hair growth and blacken hair. Can be used for treating hematemesis, epistaxis, hemoptysis, hematochezia, metrorrhagia, cough due to lung heat, blood heat, alopecia, and premature gray hair.

Mulberry leaf: sweet, bitter and cold. It enters lung and liver meridians. Dispelling wind-heat, clearing lung-heat, moistening dryness, removing liver-fire, and improving eyesight. Is used for treating wind-heat type common cold, lung heat type cough, dizziness and headache, conjunctival congestion and dizziness.

Preferably, the traditional Chinese medicinal materials comprise the following components in parts by mass:

28-32 parts of poria with hostwood, 8-12 parts of polygala tenuifolia, 8-12 parts of rhizoma acori graminei, 28-32 parts of vine of multiflower knotweed, 28-32 parts of dragon bone, 28-32 parts of mother-of-pearl, 28-32 parts of oyster, 28-32 parts of spina date seed, 18-22 parts of glossy privet fruit, 18-22 parts of eclipta, 18-22 parts of schisandra chinensis, 12-18 parts of albizia flower, 12-18 parts of rose, 8-12 parts of coptis chinensis, 3-8 parts of cinnamon, 8-12 parts of rush, 8-12 parts of rhizoma cyperi, 8-12 parts of gardenia, 8-12 parts of elecampane, 3-8 parts of frankincense, 13-18 parts of biota orientalis and 13-18 parts of mulberry leaf.

30 parts of poria with hostwood, 10 parts of polygala tenuifolia, 10 parts of grassleaf sweetflag rhizome, 30 parts of tuber fleeceflower stem, 30 parts of dragon bone, 30 parts of mother-of-pearl, 30 parts of oyster, 30 parts of spina date seed, 20 parts of glossy privet fruit, 20 parts of eclipta alba, 20 parts of shizandra berry, 15 parts of albizia flower, 15 parts of rose flower, 10 parts of coptis chinensis, 5 parts of cinnamon, 10 parts of rush, 10 parts of nutgrass galingale rhizome, 10 parts of gardenia, 10 parts of costustoot, 5 parts of frankincense, 15 parts of biota orientalis leaves and 15 parts of mulberry leaf.

Preferably, the pillow core further comprises buckwheat hulls as a filler. The buckwheat hull has the effects of inducing resuscitation with aromatics, tranquilizing, invigorating spleen, improving intelligence, etc., and can enhance massage on head and neck acupoints, and has auxiliary effects on insomnia, dizziness, cervical spondylosis, etc.

Preferably, the pillowcase is made of pure cotton cloth, has good hygroscopicity and air permeability, is soft and comfortable, and resists worm eating.

The preparation method of the medicine pillow comprises the following steps:

(1) Grinding the traditional Chinese medicine components into powder respectively and uniformly mixing according to the mass ratio;

(2) Processing the above raw materials, pulverizing, sieving, mixing, packaging into bags according to standard dosage, and sewing according to the rule of longitudinal and transverse lines or Chinese character 'Hui';

(3) Filling the Chinese medicinal bag into pillow core;

(4) Sealing the pillow core according to buckwheat husk;

(5) The pillow core is filled into a pillowcase, and the pillowcase is sealed to obtain the heart-clearing sleep-aiding and intelligence-improving medicinal pillow.

Preferably, the particle size of the powder in step (2) is 50-200 mesh; the mass ratio of the traditional Chinese medicine powder to the buckwheat husks in the step (4) is 5:1.

The using method of the heart-clearing, sleep-aiding and intelligence-improving medicinal pillow comprises the following steps of: the medicated pillow can be used at night, and the pillow core can be replaced every 1-3 months.

The beneficial effects are that: compared with the prior art, the invention has the following remarkable advantages: (1) The medicine pillow can effectively improve sleep quality, and the heart-clearing, sleep-aiding and intelligence-improving medicine pillow is provided with the traditional Chinese medicine bag in the pillow core, wherein the medicine pillow takes the intelligent soup, the Jiaotai pill and the two-to-one pill as the basic prescription, and the tranquilizer (dragon bone, oyster and mother pearl) and the heart-nourishing and tranquilizer (spina date seed and vine of multiflower knotweed) are used for assisting the basic prescription to play a role in tranquilization and stabilization; the flos albiziae and the flos rosae rugosae assist the second pill to play a role in tonifying liver and kidney; the rush, the gardenia and the schisandra chinensis assist Jiaotai pills play roles of clearing heart fire, relieving restlessness and calming heart and tranquilizing mind; the costustoot, the nutgrass galingale rhizome and the frankincense can play the role of the costustoot, the costustoot and the frankincense, and the fragrance is moderate; the cacumen biotae and the Sang Sheneng play roles in clearing lung and preventing and curing alopecia, and the above medicines cooperate to guide the five viscera and the six viscera, so as to achieve the purposes of tonifying kidney and liver, strengthening spleen and benefiting intelligence, clearing heart fire and tranquillizing, and ensure sleeping quality; (2) The traditional Chinese medicine composition adopts the external treatment method of traditional Chinese medicine, and the composition is optimized, so that the traditional Chinese medicine composition has the advantages of multiple components, multiple targets and multiple directions, has definite efficacy, is suitable for the life rhythm of modern people, is convenient to use, has small toxic and side effects, is easier to accept by people, and is widely applicable to people.

Drawings

FIG. 1 is a graph showing the effect of drug pillow on 5-HT content in brain tissue of insomnia mice;

FIG. 2 is a graph showing the effect of drug pillow on TNF- α content in serum of insomnia mice.

Detailed Description

The technical scheme of the invention is further described below with reference to specific embodiments.

Example 1

The traditional Chinese medicinal materials in the traditional Chinese medicinal bag comprise the following components: 25g of poria with hostwood, 5g of polygala tenuifolia, 5g of grassleaf sweetflag rhizome, 25g of fleece-flower stem, 25g of dragon bone, 25g of mother-of-pearl, 25g of oyster, 25g of wild jujube seed, 15g of glossy privet fruit, 15g of eclipta alba, 15g of shizandra berry, 10g of albizia flower, 10g of rose, 5g of coptis chinensis, 1g of cinnamon, 5g of rush, 5g of nutgrass galingale rhizome, 5g of gardenia, 5g of costustoot, 1g of frankincense, 10g of biota orientalis and 10g of mulberry leaf.

The preparation method of the medicine pillow comprises the following steps:

(2) Processing the above raw materials, pulverizing, sieving, mixing, packaging into bags according to standard dosage, and sewing according to the rule of longitudinal and transverse lines or Chinese character 'Hui'; the granularity of the powder is 50-200 meshes;

(3) Adding traditional Chinese medicine bag and buckwheat husk (the mass ratio of the medicine powder to the buckwheat husk is 5:1) into the pillow core, and sealing the pillow core;

(4) Filling the pillow core into a pillowcase, and sealing the pillowcase to obtain the sleep-aiding medicinal pillow.

The medicated pillow can be used at night, and the medicated pillow core can be replaced every 1 month.

Example 2

The tranquilization and sleep-aiding drug pillow in the embodiment 2 is different from the tranquilization and sleep-aiding drug pillow in the embodiment 1 only in that the content of each component in the traditional Chinese medicine bag is different, and the other components are the same;

28g of poria with hostwood, 8g of polygala tenuifolia, 8g of grassleaf sweetflag rhizome, 28g of fleece-flower stem, 28g of dragon bone, 28g of mother-of-pearl, 28g of oyster, 28g of spina date seed, 18g of glossy privet fruit, 18g of eclipta alba, 18g of shizandra berry, 12g of albizia flower, 12g of rose, 8g of coptis chinensis, 3g of cinnamon, 8g of rush, 8g of nutgrass galingale rhizome, 8g of gardenia, 8g of costustoot, 3g of frankincense, 13g of biota orientalis and 13g of mulberry leaf.

Example 3

The tranquilization and sleep-aiding drug pillow in the embodiment 3 is different from the tranquilization and sleep-aiding drug pillow in the embodiment 1 only in that the content of each component in the traditional Chinese medicine bag is different, and the other components are the same;

30g of poria with hostwood, 10g of polygala tenuifolia, 10g of grassleaf sweetflag rhizome, 30g of fleece-flower stem, 30g of dragon bone, 30g of mother-of-pearl, 30g of oyster, 30g of wild jujube seed, 20g of glossy privet fruit, 20g of eclipta alba, 20g of shizandra berry, 15g of albizia flower, 15g of rose, 10g of coptis chinensis, 5g of cinnamon, 10g of rush, 10g of nutgrass galingale rhizome, 10g of gardenia, 10g of costustoot, 5g of frankincense, 15g of biota orientalis and 15g of mulberry leaf.

Example 4

The tranquilization and sleep-aiding drug pillow in the embodiment 4 is different from the tranquilization and sleep-aiding drug pillow in the embodiment 1 only in that the content of each component in the traditional Chinese medicine bag is different, and the other components are the same;

32g of poria with hostwood, 12g of polygala tenuifolia, 12g of grassleaf sweetflag rhizome, 32g of fleece-flower stem, 32g of dragon bone, 32g of mother-of-pearl, 32g of oyster, 32g of wild jujube seed, 22g of glossy privet fruit, 22g of eclipta, 22g of shizandra berry, 18g of albizia flower, 18g of rose, 12g of coptis chinensis, 8g of cinnamon, 12g of rush, 12g of nutgrass galingale rhizome, 12g of gardenia, 12g of costustoot, 8g of frankincense, 18g of biota orientalis and 18g of mulberry leaf.

Test experiment 1: solid phase microextraction-mass spectrometry analysis of volatile components of heart-clearing, sleep-aiding and intelligence-improving compound

Weighing medicinal materials with prescription amount according to the compound proportion of the example 3, crushing, uniformly mixing, and sieving with a 40-mesh sieve to obtain compound mixed powder. And (3) placing 1.0g of the compound mixed powder into a 20mL headspace bottle, performing GC-MS analysis, detecting to obtain a total ion flow graph of the compound, adopting a standard spectrum library NIST library (2017) to search and identify, combining a retention index to perform qualitative analysis on volatile flavor components in the compound, and adopting a peak area normalization method to perform quantitative analysis.

Results: the HS-SPME-GC-MS method identified 75 volatile components altogether. The higher content is cinnamaldehyde (13.68%), asarone (29.30%), beta-asarone (9.18%), etc.

TABLE 1 solid phase microextraction extraction of sleep-aiding Chinese herbal Compound volatile Components

By virtue of the volatile oil characteristics of the alpha-asarone and the beta-asarone, the alpha-asarone and the beta-asarone can quickly penetrate through the blood brain barrier from blood to enter the brain to exert pharmacological activity, and the beta-asarone can be widely distributed in cortex, cerebellum, brain stem and hippocampus. Research shows that they have potential therapeutic advantages for nervous system diseases, such as insomnia, mild cognitive impairment and the like. Glutamate, the major neurotransmitter responsible for excitotoxicity, has a key effect in both nervous system diseases, insomnia and cognitive dysfunction, and alpha-asarone can regulate Ca ²⁺ Concentration, for glutamate receptor (AMPA, NMDA), transporter GLT-1, and controlled release of glutamate synaptorin protein (SYNI) to maintain glutamate concentration, alpha-asarone can also act directly on insomnia direct related protein CYP2D6. The alpha-asarone and the beta-asarone can regulate the excitatory neurotransmitter and the inhibitory neurotransmitter in the brain and maintain the balance level of the two. At the same time, the two can also play a role in inhibiting the expression of inflammatory factors, and the beta-asarone can reduce Abeta _25-35 The induced neuroblastoma inflammatory factors TNF-alpha, IL-1 beta and IL-6 levels reduce the inflammatory response. Alpha-asarone subtractionLight inflammation is the reduction of production of the inflammatory factors TNF- α, IL-1β by inhibiting NF- κB signaling pathways. The cinnamon volatile oil has anxiolytic and antidepressant effects on mice, and trans-cinnamic aldehyde is a main volatile component in cinnamon, and can participate in the antidepressant process through a 5-hydroxytryptamine energy receptor and an adrenergic receptor. Meanwhile, in vitro experimental researches show that the cinnamaldehyde can obviously inhibit the expression of proinflammatory cytokines IL-1 beta and TNF-alpha and the generation of NO, and can be used for treating neurodegenerative diseases closely related to neuroinflammation. The tunicarene is a main volatile component in the schisandra volatile oil, can strengthen the excitation and inhibition process of human cerebral cortex, and improve the sleep of people. Experimental study shows that the cinnamon can remarkably promote the synthesis of 5-hydroxytryptamine, epinephrine, dopamine and cerebral nerve neurotrophic factors, and increase the secretion of monoamine neurotransmitters and the sensitivity of the monoamine neurotransmitters, so that the HPA axis dysfunction is improved. And cinnamaldehyde can alleviate symptoms of mice with systemic inflammatory response syndrome, and reduce the levels of NO, TNF-alpha and peritoneal IL-10. The volatile components of cortex Cinnamomi can play neuroprotective role in Hippocampus and multiple pathological links by interaction with central monoaminergic nerve, inhibiting inflammatory reaction, etc.

Test experiment 2: ulaise subthreshold dose synergistic sleep experiments

Mice were successfully model-built and randomly screened for PCPA insomnia (blank, model, low, medium, high, n=10), and were dosed 9.1 times as much as adults according to 380g of drug used in example 3 of 60kg of daily drug pillow according to the body surface area method calculated from the human dose and experimental animal dose conversion of "pharmacological experimental methodology", so that the mice were dosed 57.63g/kg. 2 times (115.27 g/kg) of the equivalent dose of the drug pillow formula of the mice and the human is set as the medium dose of the experiment, 4 times of the equivalent dose is set as the high dose (230.53 g/kg), and the experimental intervention treatment is started on the next day. The control group does not need any treatment, and the crushed and packed medicine cores are paved above the squirrel cage in the high-medium-low dose group, and the using time of the medicine cores is 20:00-8:00 in the next day. Mice were tested for various indicators after 30d of continuous dosing.

Table 2 influence of drug pillow on sleep of insomnia mice (n=10)

Note that: compared with blank group, the P is less than 0.05

Compared with the blank control, the drug pillow high, medium and low dose groups can obviously shorten the sleep latency period (P < 0.05) of the aconite sugar of the mice and improve the sleep rate of the mice.

Test experiment 3: influence of the drug pillow Compound on the 5-HT, TNF-alpha content in brain tissue of insomnia mice

As shown in fig. 1, the 5-HT content in the brain tissue of mice in the model group was significantly reduced compared to the normal group; the drug pillow example 3 showed a significant increase in 5-HT levels in the high, medium and low dose groups compared to the model group.

As shown in fig. 2, TNF- α content was significantly elevated in the serum of the model group compared to the normal group. Drug pillow example 3 the TNF-alpha content in the serum of the high, medium and low dose groups was significantly reduced (P < 0.05).

The experimental research results show that the invention can shorten the sleep latency of the insomnia mice induced by the uliose, improve the sleep rate of the insomnia mice, increase the 5-HT level in the brain tissues of the mice and reduce the TNF-alpha level in serum.

Test experiment 4: 50 subjects with sleep quality degradation problems were selected, aged 18-55 years, and divided into 4 test groups and 1 control group by random number method. Giving sleep knowledge to the control group, and teaching and only filling a blank control drug pillow filled with filling cotton; the test group underwent sleep knowledge education while the drug pillow treatments in examples 1 to 4 were administered respectively. Patients were assessed for sleep quality using PSQI prior to and 4 weeks after group entry.

PSQI data analysis result statistics: the collected 50 test subjects are counted according to the PSQI value distribution, and the 5 test groups after the administration are counted in detail according to the PSQI value distribution, as shown in the following table 3; the sleep efficiency values of the 50 collected test subjects were counted, and the results are shown in table 4 below; and the sleep efficiency values of the 5 test groups were counted in detail as shown in table 5 below. Sleep efficiency value calculation formula: sleep efficiency value = (time to get up-time to get up)/sleep time × 100%.

Table 3 5 test groups distributed according to the PSQI values

Table 4 sleep efficiency value distribution for 50 subjects

Table 55 test group sleep efficiency value distribution

The PSQI is used for evaluating the sleep quality of the patient after the drug pillow is applied, the result shows that the total effective rate is 88.0%, the P is less than 0.05 compared with the PSQI before treatment, and the difference is statistically significant. The invention can effectively prolong the sleeping time of the insomnia patient, improve the sleeping efficiency and improve the sleeping disorder factors to a certain extent.

In order to ensure that the present invention has a good implementation effect, the present invention is used according to the best embodiment (i.e., embodiment 3), and the following cases after use by users are listed:

case one

Tian Mou women, 24 years old, study life, insomnia 1 month. Difficulty in falling asleep, lying at 11 o 'clock at night, feeling restlessness and disordered brain before sleeping, falling asleep after 12 o' clock, poor daytime spirit, difficulty in concentrating energy, dry mouth, bad smell in mouth, distention in stomach, pale tongue, thin coating and weak pulse. The heart-clearing sleep-aiding and intelligence-improving medicinal pillow is used, after 4 weeks, the sleep is well carried out with the diagnosis, the energy is full in the daytime during the follow-up visit, and the symptoms of dysphoria and discomfort in the stomach are relieved. After 2 months of continuous use, the test PSQI values tended to be normal.

Case two

Wang Mou A female, 20 years old, a college student with the Gramineae, insomnia for 3 years, a habit of staying up at night, 1-2 points of sleep, dreaminess, fatigue feeling in the daytime, poor memory, sweaty palm, cold and cool feet, pale tongue, white coating and weak pulse. The pillow can improve sleeping habit, and can be used as heart-clearing and sleep-aiding and intelligence-improving pillow, and after 2 weeks, the pillow can be used for improving sleep with the help of the patient, so that learning efficiency is improved, and physical quality is obviously improved. After 4 weeks, the patients have good sleep and are full of energy. After 2 months of continuous use, the test PSQI values tended to be normal.

Case three

Sun Mou Male, 39 years old, university professor, sleep early at night, wake for more than 1 year. The brain is in an excited state after long-term stay up, sleep shallowly and dreaminess, 1-2 or 4-5 points at night are easy to wake, and can not fall asleep after waking up or sleep without sinking, and the brain has the symptoms of night sleep, dark complexion, red tongue with purple smell, white and greasy coating and deep and thin pulse. Plain is prone to oral ulcers. The heart-clearing sleep-aiding and intelligence-improving medicine pillow is used, the sleep is gradually changed after 1 week and 2 weeks, the sleep quality is improved after 4 weeks, and the face color is white and moist. After 2 months of continuous use, the test PSQI value increases. The oral ulcer does not recur after 3 months of follow-up, and the patient feels comfortable.

Claims

1. An application of a traditional Chinese medicine composition for reducing TNF-alpha and increasing 5-HT by utilizing volatile components in preparing a sleep-aiding medicine pillow comprises a pillow case and a pillow core, wherein the pillow core contains traditional Chinese medicine bags, and the application is characterized in that: the traditional Chinese medicine package comprises the following components in parts by mass: 25-32 parts of poria with hostwood, 5-12 parts of polygala tenuifolia, 5-12 parts of rhizoma acori graminei, 25-32 parts of polygonum multiflorum, 25-32 parts of dragon bone, 25-32 parts of mother-of-pearl, 25-32 parts of oyster, 25-32 parts of spina date seed, 15-22 parts of glossy privet fruit, 15-22 parts of eclipta alba, 15-22 parts of schisandra chinensis, 10-18 parts of albizia flower, 10-18 parts of rose, 5-12 parts of coptis chinensis, 1-8 parts of cinnamon, 5-12 parts of rush, 5-12 parts of rhizoma cyperi, 5-12 parts of gardenia, 5-12 parts of elecampane, 1-8 parts of frankincense, 10-18 parts of biota orientalis and 10-18 parts of mulberry leaf.

2. The use of a traditional Chinese medicine composition for reducing TNF- α and increasing 5-HT by volatile components in the preparation of a sleep-aiding pillow according to claim 1, characterized in that: the traditional Chinese medicine package comprises the following components in parts by mass: 28-32 parts of poria with hostwood, 8-12 parts of polygala tenuifolia, 8-12 parts of rhizoma acori graminei, 28-32 parts of vine of multiflower knotweed, 28-32 parts of dragon bone, 28-32 parts of mother-of-pearl, 28-32 parts of oyster, 28-32 parts of spina date seed, 18-22 parts of glossy privet fruit, 18-22 parts of eclipta, 18-22 parts of schisandra chinensis, 12-18 parts of albizia flower, 12-18 parts of rose, 8-12 parts of coptis chinensis, 3-8 parts of cinnamon, 8-12 parts of rush, 8-12 parts of rhizoma cyperi, 8-12 parts of gardenia, 8-12 parts of elecampane, 3-8 parts of frankincense, 13-18 parts of biota orientalis and 13-18 parts of mulberry leaf.

3. The use of a traditional Chinese medicine composition for reducing TNF- α and increasing 5-HT by volatile components in the preparation of a sleep-aiding pillow according to claim 1, characterized in that: the pillow core also contains buckwheat husk as filler.

4. The use of a traditional Chinese medicine composition for reducing TNF- α and increasing 5-HT by volatile components in the preparation of a sleep-aiding pillow according to claim 1, characterized in that: the pillowcase is cloth with pure cotton texture, and has good hygroscopicity, air permeability, softness, comfort and insect resistance.

5. The use of a traditional Chinese medicine composition for reducing TNF- α and increasing 5-HT by volatile components in the preparation of a sleep-aiding pillow according to claim 1, characterized in that: the preparation method of the sleep-aiding medicinal pillow comprises the following steps of:

(1) The medicine pillow filler component according to claim 1, wherein each raw medicine is weighed according to parts by weight;

(2) Pulverizing the above materials into powder, packaging into a medicinal bag, and sealing to obtain a medicated bag;

(3) Adding Chinese medicinal bag and buckwheat hull into the pillow core, and sealing the pillow core;

6. The use of a traditional Chinese medicine composition for reducing TNF- α and increasing 5-HT by volatile ingredients in the preparation of a sleep-aiding pillow according to claim 5, wherein: the particle size of the powder in the step (2) is 50-200 meshes.