CN115812886A - Medicinal and edible selenium-rich composite solid beverage and preparation method thereof - Google Patents
Medicinal and edible selenium-rich composite solid beverage and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a selenium-rich composite solid beverage with homology of medicine and food and a preparation method thereof, wherein the selenium-rich composite solid beverage comprises the following raw materials in parts by weight: 6-15 parts of selenium-enriched bitter gourd, 6-15 parts of selenium-enriched rhizoma polygonati, 6-15 parts of selenium-enriched radix puerariae, 15-25 parts of selenium-enriched pawpaw, 6-15 parts of selenium-enriched cabbage, 6-15 parts of selenium-enriched broccoli and 10-20 parts of green tea, and meanwhile, the solid powder, namely the selenium-enriched composite solid beverage, is prepared by combining a modern preparation process and carrying out extraction, filtration, decoloration, concentration, crushing and packaging, and has better effects of oxidation resistance, inflammation diminishing and pain relieving.
Description
Technical Field
The invention belongs to the technical field of beverages, and particularly relates to a selenium-rich composite solid beverage with homology of medicine and food and a preparation method thereof.
Background
Selenium is one of the most important trace elements in a human body, the selenium in the human body usually plays a biological function in the form of selenoprotein, different selenoproteins have different physiological functions, the selenoprotein plays an important role in the aspects of diminishing inflammation, easing pain, regulating immunity, removing free radicals, preventing and resisting cancers, delaying body aging, antagonizing heavy metals and the like, simultaneously the selenoprotein also has the prevention and health care effects of resisting oxidation, resisting aging, improving the immunity of the human body and the like, and enjoys the reputation of 'fire species of life', 'long-life elements' and the like. Anti-oxidation is an important step in preventing aging, and free radicals or oxidants generated by the body decompose cells and tissues, affect metabolic functions, and cause various health problems. If too many oxidative free radicals can be eliminated, many of the associated diseases caused by free radicals can be prevented. Such as common cancers, arteriosclerosis, diabetes, cataract, cardiovascular disease, senile dementia, arthritis, etc., which are considered to be associated with free radicals. The antioxidant can effectively inhibit the oxidation reaction of free radicals, and is widely applied in clinical and medical fields.
However, artificially synthesized antioxidants bring people with if they are used for long-term treatment of human bodyThe toxic and side effects of the selenium-enriched compound can greatly influence the body health of people, and conflict with the concepts of health preservation, health care and the like advocated by people at present, the antioxidant substances of the human body are synthesized by the antioxidant substances, are also supplied by foods, and can cause the reduction of the immunity of the human body due to the long-term selenium deficiency, so that certain viruses and germs can invade the body to induce keshan disease, kashin-Beck disease and the like. According to WHO, the recommended selenium intake of the adult is 55 mu g.d -1 Less than 40. Mu.g.d -1 I.e. determined to be insufficient selenium intake. According to the results of 13 provinces of China general survey, more than 7 hundred million people have the situation of insufficient selenium intake.
Food is an important source of selenium intake for people. Therefore, the development of selenium-rich products can improve the selenium intake through daily diet, and has important significance for improving the health level of the resident population in the selenium-deficient areas. With the development of social economy and the enhancement of health consciousness of people, selenium-rich products represented by functional foods are more and more popular among people, and the development and utilization of selenium resources are in the wake of unprecedented enthusiasm.
Disclosure of Invention
Aiming at the defects, the invention aims to provide the selenium-rich composite solid beverage with homology of medicine and food and the preparation method thereof.
In order to realize the purpose, the invention adopts the following technical scheme:
the selenium-rich composite solid beverage with homology of medicine and food comprises the following raw materials in parts by weight:
6-15 parts of selenium-rich bitter gourd, 6-15 parts of selenium-rich rhizoma polygonati, 6-15 parts of selenium-rich radix puerariae, 15-25 parts of selenium-rich pawpaw, 6-15 parts of selenium-rich cabbage, 6-15 parts of selenium-rich broccoli and 10-20 parts of green tea.
Further, the selenium-rich composite solid beverage comprises the following raw materials in parts by weight:
6 parts of selenium-rich bitter gourd, 8 parts of selenium-rich rhizoma polygonati, 10 parts of selenium-rich radix puerariae, 10 parts of selenium-rich pawpaw, 10 parts of selenium-rich cabbage, 15 parts of selenium-rich broccoli and 20 parts of green tea.
Further, a preparation method of the selenium-rich composite solid beverage with homology of medicine and food, the method is characterized by comprising the following specific steps:
1) Weighing: weighing cleaned and dried selenium-rich bitter gourd, selenium-rich rhizoma polygonati, selenium-rich radix puerariae, selenium-rich pawpaw, selenium-rich cabbage, selenium-rich broccoli and green tea according to parts by weight;
2) Crushing raw materials: respectively crushing the selenium-rich bitter gourd, the selenium-rich rhizoma polygonati, the selenium-rich radix puerariae, the selenium-rich pawpaw, the selenium-rich cabbage, the selenium-rich broccoli and the green tea weighed in the step 1) into small powder particles in a sterile room, and sieving the small powder particles by a sieve of 100-200 meshes to obtain mixed powder of the raw materials;
3) Extraction: adding 95% edible ethanol into the mixed powder obtained in the step 2), soaking and extracting for 8 hours, combining the filtrates after three times of extraction, and filtering by using a 100-mesh filter bag packed centrifuge to obtain a filtrate I;
4) And (3) decoloring: decoloring the filtrate I obtained in the step 3) by using an activated carbon column to obtain a filtrate II;
5) And (3) concentrating: vacuum-pumping and concentrating the filtrate II obtained in the step 4) until the relative density is 1.08-1.22, and then cooling the filtrate II at room temperature in a sterile state to obtain extract dry paste;
6) Crushing the dry paste: crushing the dry extract paste in the step 5), and sieving the crushed dry extract paste with a 100-mesh sieve to obtain solid powder, namely the selenium-rich composite solid beverage.
Further, the dosage of the 95% edible ethanol in the step 3) is 95% of the mass of the edible ethanol: mixed powder mass = 12;
the vacuum concentration in the step 5) is carried out under the conditions that the temperature is 70 ℃ and the vacuum degree is-0.06 Mpa.
The selenium-enriched composite solid beverage with homology of medicine and food has scientific formula collocation, has the synergistic interaction effect among the components, and further explains the technical scheme of the invention by combining the components of the formula:
the selenium-rich balsam pear, the selenium-rich rhizoma polygonati, the selenium-rich radix puerariae, the selenium-rich pawpaw, the selenium-rich cabbage, the selenium-rich broccoli, the green tea and other medicinal and edible components in the invention have the functions of oxidation resistance, inflammation diminishing and the like.
Selenium-rich bitter gourd: gan Weigu is cold and cool, and active ingredients include triterpenes, alkaloids, steroid compounds, organic acids and the like.
Selenium-rich rhizoma polygonati: the production period from seedling raising to harvesting is seven years, the nature and taste are sweet and mild, and the sealwort is rich in steroid saponin, sealwort polysaccharide and sealwort oligosaccharide, can tonify qi and nourish yin, invigorate spleen and moisten lung, and has the functions of enhancing immune function, resisting aging, resisting anoxia, resisting fatigue and the like.
Selenium-rich radix puerariae: the production period from seedling raising to harvesting is three years, the tea is a traditional bulk Chinese medicinal material and a plant resource used as both medicine and food in China, the tea is rich in starch and isoflavone substances, and the isoflavone substances can reduce vascular resistance, improve brain and coronary circulation, reduce myocardial oxygen consumption and the like, so the tea has the effects of relieving muscles and allaying fever, promoting fluid production, promoting eruption, invigorating yang, relieving cough and the like.
And (3) selenium-rich pawpaw: the production cycle from seedling raising to harvesting is five years, the tea has warm and sour properties, calms the liver and harmonizes the stomach, has strong oxidation resistance, helps the organism to repair tissues, eliminates toxic substances, and enhances the immunity of the human body.
Selenium-rich cabbage: has rich antioxidant components, and can enhance the detoxification capability of enzyme system in vivo, neutralize the damage of toxin to DNA, and prevent cancer cell metastasis.
Selenium-rich broccoli: is rich in vitamin C, is a strong antioxidant component, can identify and remove free radicals in vivo, ensure human health and delay aging, and has the effects of maintaining beauty, keeping young, resisting aging, lowering blood pressure, enhancing immunity of organism, etc.
Green tea: the oxidative polymerization component containing polyphenols and catechin not only can increase the antioxidant effect of the composition, but also can harmonize fragrance.
By adopting the scheme, the invention has the following advantages:
1. according to the invention, according to the theory of homology of medicine and food, seven medicinal and edible homologous substances, namely the selenium-rich balsam pear, the selenium-rich rhizoma polygonati, the selenium-rich radix puerariae, the selenium-rich pawpaw, the selenium-rich cabbage, the selenium-rich broccoli and the green tea are subjected to compatibility and formulation, and meanwhile, the modern preparation process is combined, and the solid powder, namely the selenium-rich composite solid beverage, is prepared by extraction, filtration, decoloration, concentration, crushing and packaging. In addition, the selenium-rich composite solid beverage has the advantages of simple preparation process, wide raw material source and relatively low equipment requirement, and is convenient for large-scale industrial production.
2. The selenium-enriched composite solid beverage disclosed by the invention is extracted by edible ethanol in the preparation process, so that the effective components in the formula can be fully released, the biological activity is improved, and the solid powder has better effects of resisting oxidation, diminishing inflammation and relieving pain.
3. The seven medicinal and edible substances in the invention are low in sugar content, are suitable for low-sugar requirements of the market on products, and all contain rich antioxidant active substances. Through in vitro experimental evaluation, the selenium-rich composite solid beverage prepared by the process has obvious antioxidant, anti-inflammatory and analgesic activities.
4. The selenium-rich composite solid beverage is 10g per bag, the bag is opened and poured into a water cup, 200mL of hot water with the temperature of 70 ℃ is added, and the selenium-rich composite solid beverage can be drunk after being stirred to be completely dissolved and is convenient to carry and eat.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the invention may be realized and attained by the means of the instrumentalities and combinations particularly pointed out hereinafter.
Drawings
FIG. 1 is a diagram of DPPH free radical scavenging ability of selenium-enriched composite solid beverage.
FIG. 2 is a selenium-enriched composite solid beverage pair H 2 O 2 Morphology of induced LO2 cells.
FIG. 3 is a graph of fluorescence intensity measurement of active oxygen in selenium-enriched composite solid beverage.
FIG. 4 is a graph of the acetic acid writhing effect of the selenium-enriched compound solid beverage compound.
Detailed Description
The present invention will now be described in detail with reference to the drawings and examples, which are not intended to limit the invention in any way, except as specifically stated, reagents, methods and apparatus are conventional in the art.
Example 1: preparation of selenium-rich composite solid beverage with homology of medicine and food
The method comprises the following specific steps:
1) Weighing: weighing, cleaning and drying raw materials, namely 6 parts of selenium-enriched bitter gourd, 10 parts of selenium-enriched rhizoma polygonati, 10 parts of selenium-enriched radix puerariae, 15 parts of selenium-enriched pawpaw, 8 parts of selenium-enriched cabbage, 8 parts of selenium-enriched broccoli and 15 parts of green tea, in parts by weight;
2) Crushing raw materials: respectively crushing the raw materials weighed in the step 1) into small powder particles in a sterile room, and sieving the small powder particles by a 100-200-mesh sieve to obtain mixed powder of the raw materials, wherein the sterile and clean states are kept;
3) Extraction: adding 95% edible ethanol into the mixed powder in the step 2), soaking and extracting for 8 hours, wherein the dosage of the ethanol is 95% of the mass of the edible ethanol: extracting the mixed powder for three times, combining the filtrates, and filtering by using a 100-mesh filter bag centrifuge to obtain a filtrate I, wherein the mass of the mixed powder is = 12;
4) And (3) decoloring: decoloring the filtrate I obtained in the step 3) by using an activated carbon column, and further filtering to obtain a filtrate II;
5) And (3) concentrating: vacuum-pumping and concentrating the filtrate II obtained in the step 4) until the relative density is 1.12, wherein the concentration condition is that the temperature is 70 ℃, the vacuum degree is-0.06 Mpa, and then placing the filtrate II at room temperature and cooling the filtrate II in a sterile state to obtain dry paste of the extract;
6) Crushing the dry paste: crushing the dry extract paste in the step 5), and sieving the crushed dry extract paste with a 100-mesh sieve to obtain solid powder, namely the selenium-rich composite solid beverage;
7) Packaging: packaging the solid powder obtained in the step 6) by using food grade plastics under vacuum condition, wherein each bag is packaged by 10g.
Example 2: preparation of selenium-rich composite solid beverage with homology of medicine and food
The method comprises the following specific steps:
1) Weighing: weighing, cleaning and drying raw materials according to parts by weight, namely 6 parts of selenium-rich bitter gourd, 8 parts of selenium-rich rhizoma polygonati, 10 parts of selenium-rich radix puerariae, 10 parts of selenium-rich pawpaw, 10 parts of selenium-rich cabbage, 15 parts of selenium-rich broccoli and 20 parts of green tea;
2) Crushing raw materials: respectively crushing the raw materials weighed in the step 1) into small powder particles in a sterile room, and sieving the small powder particles by a 100-200-mesh sieve to obtain mixed powder of the raw materials, wherein the sterile and clean states are kept;
3) Extraction: adding 95% edible ethanol into the mixed powder in the step 2), soaking and extracting for 8 hours, wherein the dosage of the ethanol is 95% of the mass of the edible ethanol: extracting the mixed powder for three times, combining the filtrates, and filtering by using a 100-mesh filter bag centrifuge to obtain a filtrate I, wherein the mass of the mixed powder is = 12;
4) And (3) decoloring: decoloring the filtrate I obtained in the step 3) by using an activated carbon column, and further filtering to obtain a filtrate II;
5) Concentration: vacuum-pumping and concentrating the filtrate II obtained in the step 4) until the relative density is 1.12, wherein the concentration condition is that the temperature is 70 ℃, the vacuum degree is-0.06 Mpa, and then placing the filtrate II at room temperature and cooling the filtrate II in a sterile state to obtain dry paste of the extract;
6) Crushing the dry paste: crushing the dry extract in the step 5), and sieving the crushed dry extract with a 100-mesh sieve to obtain solid powder, namely the selenium-rich composite solid beverage;
7) Packaging: packaging the solid powder obtained in the step 6) by using food grade plastics under vacuum condition, wherein each bag is packaged by 10g.
Example 3: quality evaluation of selenium-rich composite solid beverage
1. Experimental Material
The selenium-enriched composite solid beverage prepared under the conditions of the embodiment 2.
2. Experimental methods
Appearance evaluation: the selenium-enriched composite solid beverage in the embodiment 2 is taken for visual observation;
determination of solubility: taking 10g of the selenium-rich composite solid beverage powder in the embodiment 2, adding 200mL of hot water at 70 ℃, stirring for 3min by using a glass rod, and immediately observing that soluble powder is completely dissolved or slightly turbid;
determination of dissolution time: 10g of the selenium-enriched composite solid beverage powder obtained in example 2 was added to 100mL of water at 25 ℃ and gently stirred with a glass rod, and the time required for complete dissolution was recorded.
3. Results of the experiment
The selenium-enriched composite solid beverage is light yellow in appearance, loose in form, free of agglomeration, uniform in powder thickness, proper in particle size, good in color and luster and good in overall impression;
when the solubility is measured, the water can be quickly and completely dissolved and dispersed in hot water with the temperature of more than 70 ℃, the measured dissolving time is less than 40s, the solution is light yellow to yellow in color, and the water has no precipitation and layering phenomena after standing, has the fragrance of raw materials, sweet taste and slightly sour taste, and is safe to eat.
Example 4: bacterium detection of selenium-rich composite solid beverage
1. Experimental materials
The selenium-enriched composite solid beverage prepared under the conditions of the example 2.
2. Experimental methods
And (4) determining the total number of colonies by a reference national standard GB 47889.2-2010 colony count determination method, namely a plate culture method. 10g of the selenium-enriched compound solid beverage prepared under the conditions of example 2 is filled into a sterile homogenizing bag containing 100mL of physiological saline to prepare a sample solution of 1. According to the principle design that each living bacterium can grow a colony, 1mL of 1: 10. 1, 100, 1. Meanwhile, 1mL of the blank diluent is sucked and added into 3 sterile plates to serve as a blank control, and 1mL of the mixed standard bacterial liquid is sucked and added into 3 sterile plates to serve as a positive control. Adding agar culture medium, mixing, culturing at 37 deg.C for 48 hr, and counting viable bacteria according to the number of cultured colonies.
3. Results of the experiment
The total colony count (CFU/g) of the selenium-rich composite solid beverage is less than or equal to 1000, the coliform group (MPN/g) is less than or equal to 0.4, the mould (CFU/g) is less than or equal to 25, the microzyme (CFU/g) is less than or equal to 25, and the salmonella, shigella and staphylococcus aureus are detected.
Example 5: determination of DPPH free radical scavenging ability of selenium-enriched composite solid beverage
1. Experimental Material
The selenium-enriched composite solid beverage prepared under the conditions of the example 2.
2. Experimental methods
Accurately weighing selenium-rich composite solid beverage powder samples, preparing solutions with concentration gradients of 50 mu g/mL, 100 mu g/mL, 200 mu g/mL, 500 mu g/mL, 1000 mu g/mL and 2000 mu g/mL by using absolute ethyl alcohol, taking 100 mu L of sample solution with a certain concentration, adding 100 mu L of 0.2M DPPH solution, immediately mixing uniformly, placing in the dark at room temperature for 30min, and measuring the absorbance value at the wavelength of 517 nm. The smaller the light value, the stronger the radical scavenging ability is indicated. The blank group was prepared by using absolute ethanol instead of the sample solution, and vitamin C was used as a positive control, and the clearance rate was calculated by the following formula for each sample in triplicate:
clearance (%) = [1- (a) s -A sb )/A b ]×100
A b Blank absorbance, A S Absorbance of the DPPH solution after sample addition, A sb The absorbance of the sample itself (without the addition of DPPH solution).
3. Results of the experiment
Through experimental statistics, the DPPH free radical scavenging capacity of each dose group of the compound solid beverage is shown in Table 1. The result shows that the DPPH clearance rate of the positive control vitamin C reaches more than 90 percent when the concentration of the positive control vitamin C is 2.5 mu g/mL. The DPPH clearance of the sample of the compound solid beverage is 32.28 percent at 50 mu g/mL, and the DPPH clearance is increased along with the increase of the concentration of the sample at 50-1000 mu g/mL. The DPPH clearance rate is stabilized to be more than 90 percent between 1000 and 2000 mu g/mL. The selenium-rich composite solid beverage has certain DPPH free radical scavenging capacity and shows certain dose-effect relationship, and the selenium-rich composite solid beverage has good antioxidation.
TABLE 1 DPPH radical scavenging Table for each dose group of composite solid beverages
Example 6: selenium-rich composite solid beverage pair H 2 O 2 Induction of LO 2 Influence of cell morphology
1. Experimental Material
The selenium-enriched composite solid beverage prepared under the conditions of the example 2.
2. Experimental methods
Experimental grouping conditions: blank control group, H 2 O 2 A modeling group, an NAC positive control group and a sample group to be determined. The concentration of the sample to be measured is 50 mug/mL, 30 mug/mL, 20 mug/mL, 15 mug/mL, 10 mug/mL and 5 mug/mL;
collecting LO2 cells in logarithmic growth phase and good growth state, digesting, counting, inoculating into 24-well plate with cell number of 0.4 × 10 5 Adding RPMI-1640 complete cell culture medium to 500. Mu.L, and culturing in a cell culture box for 24 hr;
the stock samples were diluted with medium to the concentration of the sample to be assayed (50, 30, 20, 15, 10 and 5. Mu.g/mL), 500. Mu.L of sample was added to each well, and the 24-well plate was placed further in the cell incubator for 24H (blank, H) 2 O 2 The mould making group is only added with culture medium; NAC positive control group plus 4mM NAC);
remove drug and NAC, wash twice with PBS, add H to all groups 2 O 2 Acting for 30min, discarding H 2 O 2 Washed twice with PBS, stained with 10. Mu.M DCFH-DA for 30min in each well, protected from light at room temperature, washed 2 times with 300. Mu.L PBS in each well, aspirated, and photographed under a fluorescent microscope (magnification 200X).
3. Results of the experiment
The results are shown in FIG. 2, which is detailed belowSpent in cell H 2 O 2 (1 mM) treatment for 30min in molding 2 O 2 The fluorescence intensity of the molding group is obviously stronger than that of the blank control group, which indicates that the molding is successful. The fluorescence intensity of each sample group to be measured is obviously lower than that of the model group and is close to that of the blank control group and the NAC positive control group, which shows that the selenium-enriched composite solid beverage with the fluorescence intensities of 50 mug/mL, 30 mug/mL, 20 mug/mL, 15 mug/mL, 10 mug/mL and 5 mug/mL has obvious antioxidant activity.
Example 7: selenium-rich composite solid beverage pair H 2 O 2 Induction of LO 2 Influence of intracellular reactive oxygen species
1. Experimental materials
Selenium-enriched composite solid beverage prepared under the conditions of example 2
2. Experimental methods
Experimental grouping conditions: blank control group, H 2 O 2 A modeling group, an NAC positive control group and a sample group to be determined. The concentration of the sample to be measured is 20 mug/mL, 15 mug/mL, 10 mug/mL and 5 mug/mL;
detecting the fluorescence intensity of active oxygen in the cells by a fluorescence microplate reader:
collecting LO2 cells in logarithmic growth phase and good growth state, digesting, counting, inoculating into 24-well plate with cell number of 0.4 × 10 5 Culturing for 24 hours;
the stock solution was diluted with the medium to the concentration of the sample to be assayed, 500. Mu.L of the sample was added to each well, and the 24-well plate was further placed in a cell incubator for 24 hours (blank control, H) 2 O 2 The mould making group is only added with culture medium; NAC positive control group plus 4mM NAC);
removing the drug and NAC, washing with PBS twice, staining with DCFH-DA dye (final concentration 10 μ M) for 30min, and washing with PBS 2 times;
mu.L of lysate (DMSO: PBS = 9:1) was added to each well of 24-well plate, and incubated for 10min on a light-shielding shaker at room temperature;
adding the lysis solution in the 24-well plate into 3 wells of a 96-well plate with a black frame transparent bottom respectively, wherein each well is 100 mu L;
the fluorescence intensity was measured by a fluorescent microplate reader under the conditions of 488nm excitation wavelength and 520nm emission wavelength.
3. Results of the experiment
The results are shown in FIG. 3, cell depletion H 2 O 2 Treating for 30min in the molding process (1 mM) 2 O 2 The fluorescence intensity of the molding group is obviously stronger than that of the blank control group, which indicates that the molding is successful. The fluorescence intensity of each sample group to be measured is obviously lower than that of the model group and is close to that of the blank control group and the NAC positive control group, which shows that the selenium-enriched composite solid beverage with the concentration of 20 mug/mL, 15 mug/mL, 10 mug/mL and 5 mug/mL can reverse H 2 O 2 Inducing ROS in LO2 cell to rise, and having obvious antioxidant activity
Example 8: anti-inflammatory analgesic activity of selenium-rich composite solid beverage
1. Experimental Material
The selenium-enriched composite solid beverage prepared under the conditions of the embodiment 1 and the embodiment 2.
2. Experimental methods
Experimental animals:
KM mice, male and female halves, standard of cleaning grade, total 100 mice, body weight about 18-22g, randomly divided into ten groups of 10 mice each. Three dose groups of blank control group (0.9% NaCl solution), positive control group (morphine solution) and compound solid beverage are set. Three dose groups of the composite solid beverage obtained in the above examples 1 and 2 are respectively marked as A1, A2, A3, B1, B2 and B3;
sample source and preparation:
high dose groups (A1 and B1): the compound solid beverage particles are diluted by adding normal saline, the content of crude drugs is 25mg/mL, and the administration dosage is 250mg/Kg.
Medium dose group (A2 and B2): the compound solid beverage particles are diluted by adding normal saline, the content of crude drugs is equivalent to 5mg/mL, and the administration dosage is equivalent to 50mg/Kg.
Low dose group (A3 and B3): the compound solid beverage particles are diluted by adding normal saline, the content of crude drugs is 1mg/mL, and the administration dosage is 10mg/Kg.
Positive control groups (M1 to M4): the positive drug is morphine hydrochloride solution, and the concentration gradients are respectively set to be 5mg/Kg, 1mg/Kg, 0.2mg/Kg and 0.04mg/Kg.
Acetic acid-induced writhing experiments in mice:
mice were fasted for 12h before the experiment, and then each group of mice was gavaged at 0.7mL/20g body weight, with the model control group given an equal volume of distilled water. 30min after administration, 0.2mL of 0.6% acetic acid solution is injected into the abdominal cavity, the times of writhing within 30min are observed and recorded, and the analgesic rate is calculated.
Mice will show a more pronounced writhing response in pain models, while active ingredients with analgesic effect will reduce writhing frequency. The mice have the behaviors of abdominal indent, trunk and hind limb extension, hip rising and the like, which is called a complete writhing reaction.
The blank control group is 0.9% NaCl solution, and the positive drug group is morphine solution. Each mouse was independently observed in an iron mesh cage, and after 30 minutes of intraperitoneal injection of a different dose of the sample solution to be measured, 0.8% v/v acetic acid solution (0.1 ml/10 g) was intraperitoneally injected, and the number of writhing of the mouse was recorded for 30 minutes, and 10 random mice were used for each set of experiments to conduct repetitive experiments.
Analgesic rate = [ (blank control group writhing times-administration group writhing times)/blank control group writhing times ] × 100%
3. Results of the experiment
As shown in fig. 4 and table 2, the experimental groups were counted by GraphPad Prism, and the dose groups of the selenium-enriched compound solid beverages obtained in examples 1 and 2 had significant analgesic effects in the acetic acid-induced writhing experiment in mice compared to the normal model group. The effect of the high-dose group of the compound solid is equivalent to that of morphine in the positive control group, the analgesic inhibition rate of the high-dose group in example 1 is 92.3%, and the analgesic inhibition rate of the high-dose group in example 2 is 97.7%, which indicates that the selenium-enriched compound solid beverage has obvious anti-inflammatory and analgesic effects.
TABLE 1 results of analgesic experiments in each experimental group (X. + -. S, n = 10)
| Group of | Dosage to be administered | Number of times of body twisting | Inhibition rate |
| Model set | 0.1mL/10g | 43.1±1.5 | - |
| Positive control group M1 | 5mg/ |
0*** | 100％ |
| Positive control group M2 | 1mg/Kg | 9.4±1.6*** | 78.2％ |
| Positive control group M3 | 0.2mg/Kg | 13.8±1.9*** | 68.1％ |
| Positive control group M4 | 0.04mg/Kg | 19.8±2.2*** | 54.1％ |
| Compound high dose group A1 | 250mg/Kg | 3.3±2.1*** | 92.3％ |
| Compound middle dose group A2 | 50mg/Kg | 10.5±0.7** | 75.6％ |
| Compound low dose group A3 | 10mg/Kg | 14.0±2.2*** | 67.5％ |
| Compound high dose group B1 | 250mg/Kg | 1.0±0.0*** | 97.7％ |
| Compound middle dose group B2 | 50mg/Kg | 6.4±0.9*** | 85.2％ |
| Compound low dose group B3 | 10mg/Kg | 11.5±3.6** | 73.3％ |
Comparing to model group, p <0.001, p <0.01.
Finally, the description is as follows: although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art will understand that various changes, modifications and substitutions can be made without departing from the spirit and scope of the invention as defined by the appended claims. Any modification, equivalent replacement, or improvement made without departing from the spirit and principle of the present invention shall fall within the protection scope of the present invention.
Claims (4)
1. The selenium-rich composite solid beverage with homology of medicine and food is characterized by comprising the following raw materials in parts by weight:
6-15 parts of selenium-rich bitter gourd, 6-15 parts of selenium-rich rhizoma polygonati, 6-15 parts of selenium-rich radix puerariae, 15-25 parts of selenium-rich pawpaw, 6-15 parts of selenium-rich cabbage, 6-15 parts of selenium-rich broccoli and 10-20 parts of green tea.
2. The selenium-rich composite solid beverage as both medicine and food according to claim 1, wherein the selenium-rich composite solid beverage comprises the following raw materials in parts by weight:
6 parts of selenium-rich bitter gourd, 8 parts of selenium-rich rhizoma polygonati, 10 parts of selenium-rich radix puerariae, 10 parts of selenium-rich pawpaw, 10 parts of selenium-rich cabbage, 15 parts of selenium-rich broccoli and 20 parts of green tea.
3. The preparation method of the selenium-enriched composite solid beverage with homology of medicine and food according to any one of claims 1-2, which is characterized by comprising the following specific steps:
1) Weighing: weighing cleaned and dried selenium-rich bitter gourd, selenium-rich rhizoma polygonati, selenium-rich radix puerariae, selenium-rich pawpaw, selenium-rich cabbage, selenium-rich broccoli and green tea according to parts by weight;
2) Crushing raw materials: respectively crushing the selenium-rich bitter gourd, the selenium-rich rhizoma polygonati, the selenium-rich radix puerariae, the selenium-rich pawpaw, the selenium-rich cabbage, the selenium-rich broccoli and the green tea weighed in the step 1) into small powder in a sterile room, and sieving the small powder with a 100-200-mesh sieve to obtain mixed powder of the raw materials;
3) Extraction: adding 95% edible ethanol into the mixed powder obtained in the step 2), soaking and extracting for 8 hours, combining the filtrates after three times of extraction, and filtering by using a 100-mesh filter bag packed centrifuge to obtain a filtrate I;
4) And (3) decoloring: decoloring the filtrate I obtained in the step 3) by using an activated carbon column to obtain a filtrate II;
5) Concentration: vacuum-pumping and concentrating the filtrate II obtained in the step 4) until the relative density is 1.08-1.22, and then cooling the filtrate II at room temperature in a sterile state to obtain extract dry paste;
6) Crushing the dry paste: crushing the dry extract paste in the step 5), and sieving the crushed dry extract paste with a 100-mesh sieve to obtain solid powder, namely the selenium-rich composite solid beverage.
4. The preparation method of the selenium-enriched composite solid beverage with homology of medicine and food according to claim 3, wherein the amount of the 95% edible ethanol in the step 3) is 95% by mass of the edible ethanol: mixed powder mass = 12;
the vacuum concentration in the step 5) is carried out under the conditions that the temperature is 70 ℃ and the vacuum degree is-0.06 Mpa.
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