CN110301567A - A kind of red acid slurry Siraitia grosvenorii throat-clearing throat-moistening beverage and preparation method thereof - Google Patents
A kind of red acid slurry Siraitia grosvenorii throat-clearing throat-moistening beverage and preparation method thereof Download PDFInfo
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- CN110301567A CN110301567A CN201910649271.2A CN201910649271A CN110301567A CN 110301567 A CN110301567 A CN 110301567A CN 201910649271 A CN201910649271 A CN 201910649271A CN 110301567 A CN110301567 A CN 110301567A
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Mycology (AREA)
- Medicines Containing Plant Substances (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention belongs to technical field of beverage preparation, disclose a kind of red acid slurry Siraitia grosvenorii throat-clearing throat-moistening beverage and preparation method thereof, select fresh Loquat-Shaped Cake, pyrus nivalis is homogenized, and to Siraitia grosvenorii best in quality, honeysuckle, peppermint, reed root, dried orange peel, Schisandra chinensis crushes after being dried, smashed powder proportionally adds 10 times of water progress hot dippings twice to mention, filtering, and then above-mentioned food materials effective component is extracted, gained extracting solution 4000rpm centrifugation 15min takes clear liquid, Loquat-Shaped Cake after homogenate is added, pyrus nivalis and tremella juice, filtrate after centrifugation, red acid berry juice carries out compounding cold fermentation after-ripening in proportion, sterilization packaging after allotment.The present invention develops the throat-clearing throat-moistening beverage using red acid berry as primary raw material, the bacteriostasis for improving beverage is handled by process optimization, it improves using red acid berry as the stability of the throat-clearing throat-moistening beverage of primary raw material, product has preferable Social benefit and economic benefit.
Description
Technical field
The invention belongs to technical field of beverage preparation more particularly to a kind of red acid slurry Siraitia grosvenorii throat-clearing throat-moistening beverage and its systems
Preparation Method.
Background technique
Currently, the immediate prior art:
Red acid berry also known as red Miss are the health wild fruits of medicine food two, and fruit clothing, cauline leaf are extremely bitter, and herb can be used as medicine,
Fruit can be eaten raw, rich in citric acid, oxalic acid, Vc, carotenoid composition, physalin and wintercherry sterol A, B etc., modern times doctor
It learns research shows that red Miss can be with clearing heat and eliminating phlegm, reducing pharyngeal swelling.
Loquat-Shaped Cake Chinese ancient name reed tangerine, also known as golden ball, Lu Zhi, the various nutrients rich in needed by human body, are nutrition
Health fruit can treat lung heat cough and asthma, spit inverse, polydipsia, moistening lung to lower qi.
Pyrus nivalis, it is medicinal to control wind-heat, moistening lung, the cool heart, dissolving phlegm, fall fire, removing toxic substances.Medical research proves that pears truly have moistening lung
Clear dry, relieving cough and reducing sputum, nourishing blood and promoting granulation effect.
Siraitia grosvenorii is one of the dual-purpose of drug and food material that country ratifies in the first batch, and primary efficacy is can be relieving cough and reducing sputum, and there are also clear
Pyrolysis heat, preventing phlegm from forming and stopping coughing, cool blood, which relax, bone, clearing lung-heat ease constipation and to promote the production of body fluid to quench thirst and other effects.
Dandelion alias grandmother's fourth, is mainly used for treating furunculosis swelling and pain, and female red, pharynx pain can be clearing heat and detoxicating, and dissipating bind is swollen clearly,
And it can inducing diuresis for treating strangurtia.
Reed root controls lung heat, cough, lung carbuncle pyemesis.Orange peel is also known as dried orange peel, citrus chachiensis hortorum, has regulating qi-flowing for strengthening spleen, eliminating dampness and eliminating phlegm
The effect of.
The diseases such as influenza, headache, hot eyes, body heat, throat, gum swelling and pain are controlled " lunar caustic grass " in peppermint, popular name.
Honeysuckle also known as be honeysuckle, have the effect of resisting pathogenic microbes and anti-inflammatory.
Mulberry leaf are a kind of dual-purpose of drug and food resources, have dispelling wind and heat from the body, clearing away the lungheat and moisturizing, clear liver and improve vision, dizziness headache, hot eyes dusk
Flower and other effects.
Schisandra chinensis has the effect of astringency inducing, nourishing generate fluid, kidney calming.Radix Glycyrrhizae is invigorated the spleen and benefited qi, clearing heat and detoxicating, eliminating the phlegm
Cough-relieving, relieving spasm to stop pain, coordinating the drug actions of a prescription.In recent years due to environmental degradation, air pollution, or since rhythm of life is accelerated, work is pressed
The excessive drinking and smoking of the reasons formation such as power increase, irregular, tired, nervous, passive protective physical fitness decline of living for a long time, all may
Lead to the appearance of laryngopharyngitis.
Clinical observation shows that sphagitis is up to 87% or more in crowd's disease incidence.Have become the general of puzzlement people's daily life
All over one of property disease.
In conclusion problem of the existing technology is:
In the prior art, health wild fruit of the red acid berry as medicine food two, has clearing heat and eliminating phlegm, the function of reducing pharyngeal swelling
Effect.But its deep processing and utilization is less, is mostly sold with fresh and dried and food materials raw material.Therefore, red acid berry has utilization rate lower；And
And existing beverage cannot promote red acid berry, Loquat-Shaped Cake and the high added value of pyrus nivalis, industrial upgrading；Existing drink and production process
It is cumbersome, it is inconvenient for operation.
Summary of the invention
In view of the problems of the existing technology, the present invention provides a kind of red acid slurry Siraitia grosvenorii throat-clearing throat-moistening beverage and its systems
Preparation Method.The present invention is based on traditional Chinese medicines, will have effects that the dual-purpose of drug and food sheet of antibacterial anti-inflammatory, dispelling wind and heat, inducing diuresis for treating strangurtia
Careless plant develops the composite health care beverage with throat-clearing throat-moistening function by formulation optimization
The invention is realized in this way a kind of red acid berry is the throat-clearing throat-moistening beverage of primary raw material, based on the red acid berry
The throat-clearing throat-moistening beverage of raw material is wanted to be made of major ingredient and auxiliary material；
The major ingredient is made of loquat Juice, snow pear juice, tremella juice and red acid berry juice；
The auxiliary material is made of honeysuckle juice, arhat juice, mint juice, Schisandra chinensis juice, dried orange peel juice, pharagmitis rhizoma juice；
The major ingredient, auxiliary material each component press percentage are as follows: red acid berry juice 50~60%, loquat Juice 8~12%, tremella
Juice 12~18%, snow pear juice 8~12%, arhat juice 1~3%, honeysuckle juice 2~5%, mint juice 1~3%, dried orange peel juice 0.5~
1.5%, Schisandra chinensis juice 0.5~1.5%, pharagmitis rhizoma juice 1~3%.
Another object of the present invention is to provide a kind of clearing that red acid berry as described in claim 1 is primary raw material profits
The preparation method of larynx beverage, the red acid berry are that the preparation method progress raw material of the throat-clearing throat-moistening beverage of primary raw material is located in advance
It is filling after reason, infusion, filtering, secondary extraction filtering, merging filtrate, ratio optimization, centrifugation, cold fermentation, after-ripening, sterilizing, it obtains
It is the throat-clearing throat-moistening beverage product of primary raw material to red acid berry.
Further, carrying out pretreated method to raw material includes:
The pretreatment of major ingredient: selecting fresh Loquat-Shaped Cake, pyrus nivalis, tremella, and 20% lemon juice and 2~3% foods are added in Loquat-Shaped Cake
Salting liquid impregnates 10min and carries out color protection, and the Loquat-Shaped Cake and pyrus nivalis after color protection are homogenized in the ratio of 1:1, filtering；
The pretreatment of auxiliary material: Siraitia grosvenorii best in quality, honeysuckle, peppermint, reed root, dried orange peel, Schisandra chinensis is selected to be dried, powder
It is broken；
Further, progress infusion, filtering, secondary extraction are filtered, in merging filtrate step, are specifically included: by smashed arhat
Fruit, honeysuckle, peppermint, reed root, dried orange peel, Schisandra chinensis are proportionally added into 10 times of boiling boiling 10min respectively, filter, filter residue adds 10 times
Water, 60~70 DEG C of extraction 10min, merging filtrate after filtering.
Further, it carries out ratio optimization, in centrifugation step, specifically include: by resulting fruit juice, arhat juice, honeysuckle
Juice, mint juice, pharagmitis rhizoma juice, dried orange peel juice, Schisandra chinensis juice carry out 4000r and are centrifuged 15min；
By fruit juice, arhat juice, honeysuckle juice, mint juice, pharagmitis rhizoma juice, dried orange peel juice, Schisandra chinensis juice, red acid berry juice by not year-on-year
Example is compounded.
Further, the method for carrying out cold fermentation includes:
0.1%~0.2% lactobacillus plantarum will be accessed after the sterilization of product after compounding, in 30 DEG C of incubators fermentation 48h.
Further, the method for carrying out after-ripening includes: 72h~96h under the conditions of the product after allotment is carried out 4~8 DEG C of low temperature
Save after-ripening.
Further, after being crushed in infusion, 30~45min is boiled；
Add 10 times of water, 60~70 DEG C of extraction 10min in leaching process in proportion；
Infusion, the resulting filtrate of extraction merge simultaneously.
The throat-clearing throat-moistening drink that the red acid berry is primary raw material is utilized another object of the present invention is to provide a kind of
Expect the red acid berry beverage of preparation.
In conclusion advantages of the present invention and good effect are as follows:
Select fresh Loquat-Shaped Cake, pyrus nivalis is homogenized, and to Siraitia grosvenorii best in quality, honeysuckle, peppermint, reed root, dried orange peel,
Schisandra chinensis crushes after being dried, and smashed powder proportionally adds 10 times of water progress hot dippings twice to mention, filter, and then will be upper
It states food materials effective component to extract, gained extracting solution 4000rpm centrifugation 15min takes clear liquid, Loquat-Shaped Cake, snow after homogenate is added
Filtrate, red acid berry juice after pears and tremella juice, centrifugation carry out compounding cold fermentation after-ripening, sterilization packaging after allotment in proportion.
The present invention develops the throat-clearing throat-moistening beverage using red acid berry as primary raw material, and the suppression for improving beverage is handled by process optimization
Bacterium ability is improved using red acid berry as the stability of the throat-clearing throat-moistening beverage of primary raw material, and product sensory quality is preferable, color
Golden yellow, fragrant and sweet with loquat snow pear juice, the unique flavor of red acid berry juice, mouthfeel is full, refrigerant sour-sweet, and has clearing
The value of throat soothing is a kind of ideal throat soothing beverage, has preferable Social benefit and economic benefit.
And it is provided by the invention using red acid berry as the preparation method of the throat-clearing throat-moistening beverage of primary raw material, with loquat
Fruit, pyrus nivalis, red acid slurry are primary raw material, and honeysuckle, Siraitia grosvenorii, peppermint, reed root, Schisandra chinensis, dried orange peel (can also add as needed
Appropriate Radix Glycyrrhizae, mulberry leaf, juice of dandelion) it is auxiliary material, it is prepared after pretreatment, infusion, filtering, centrifugation, compounding, extracts skill
Art is fully extracted the effective component in Chinese medicine；The rate of deposition for changing filtrate using centrifugation technique, improves the stabilization of beverage
Property, reduce rate of deposition；Health wild fruit of the red acid berry as medicine food two, has clearing heat and eliminating phlegm, the function of reducing pharyngeal swelling
Effect.But its deep processing and utilization is less, is mostly sold with fresh and dried and food materials raw material.Therefore, obtain red acid berry effective efficiency at
On the basis of point, developing more novel products is one of the important channel for improving red acid berry utilization rate.Loquat-Shaped Cake unique flavor,
Moderately sour and sweet, and the nutriment with a variety of needed by human body.Nutritional ingredient rich in loquat fruits has treatment
Lung heat cough and asthma spits the functions such as inverse, polydipsia, moistening lung to lower qi.Pyrus nivalis has effects that clear dry, the relieving cough and reducing sputum, nourishing blood and promoting granulation of moistening lung.This
Invention is using red acid berry, Loquat-Shaped Cake and pyrus nivalis as primary raw material, and present invention process is simple, easy to operate, not only develops
A kind of throat-clearing throat-moistening beverage, and promote red acid berry, Loquat-Shaped Cake and the development and utilization of the high added value of pyrus nivalis；Boosting is red simultaneously
Wintercherry fruit, Loquat-Shaped Cake and pyrus nivalis industrial upgrading.
Detailed description of the invention
Fig. 1 is red acid slurry Siraitia grosvenorii throat-clearing throat-moistening method for preparing beverage flow chart provided in an embodiment of the present invention.
Fig. 2 is the biocidal property lab diagram of red acid slurry Siraitia grosvenorii throat-clearing throat-moistening beverage provided in an embodiment of the present invention.In figure:
(a) biocidal property experiment one.(b) biocidal property experiment two.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
In the prior art, health wild fruit of the red acid berry as medicine food two, has clearing heat and eliminating phlegm, reducing pharyngeal swelling
The effect of.But its deep processing and utilization is less, is mostly sold with fresh and dried and food materials raw material.Therefore, red acid berry have utilization rate compared with
It is low；And existing beverage cannot promote red acid berry, Loquat-Shaped Cake and the high added value of pyrus nivalis, industrial upgrading；Existing beverage system
Standby technique is cumbersome, inconvenient for operation.
To solve the above problems, the present invention is described in detail with reference to the accompanying drawing.
Red acid slurry Siraitia grosvenorii throat-clearing throat-moistening beverage provided in an embodiment of the present invention is made of major ingredient and auxiliary material；
The major ingredient is made of loquat Juice, snow pear juice, tremella juice and red acid berry juice；
The auxiliary material is by honeysuckle juice, arhat juice, mint juice, Schisandra chinensis juice, succus liquiritiae, dried orange peel juice, juice of dandelion, pharagmitis rhizoma juice
And white mulberry vein-juice composition；
The each component percentage of the major ingredient, auxiliary material are as follows: red acid berry juice 50~60%, loquat Juice 8~12%, tremella juice
12~18%, snow pear juice 8~12%, arhat juice 1~3%, honeysuckle juice 2~5%, mint juice 1~3%, dried orange peel juice 0.5~1.5%,
Schisandra chinensis juice 0.5~1.5%, pharagmitis rhizoma juice 1~3%.
As shown in Figure 1, red acid provided in an embodiment of the present invention starches Siraitia grosvenorii throat-clearing throat-moistening drink and production process process are as follows:
(1) secondary extraction filtering → (5) merging filtrate → (6) proportion in pretreatment of raw material → (2) infusion → (3) filtering → (4) is excellent
Change → (7) centrifugation → (8) packaging → (9) finished product.
The preparation method of red acid slurry Siraitia grosvenorii throat-clearing throat-moistening beverage provided in an embodiment of the present invention specifically includes:
(1) raw material are pre-processed:
Major ingredient: selecting fresh Loquat-Shaped Cake, pyrus nivalis, while 20% lemon juice and 1% salt solution immersion 10min being added in Loquat-Shaped Cake
Color protection is carried out, the Loquat-Shaped Cake and pyrus nivalis after color protection are homogenized in the ratio of 1:1, filtering.
Auxiliary material: Chinese medicine best in quality is selected to be dried, crushed；
(2) smashed Chinese medicine is proportionally added into 10 times of boiling boiling 10min, filtered, filter residue adds 10 times of water, 60~70 DEG C of extractions
10min, merging filtrate after filtering；
(3) step (1), the resulting fruit juice of step (2), decoction are subjected to 4000r and are centrifuged 15min；
(4) fruit juice, decoction, red acid berry wine are compounded in proportion；
(5) product after allotment is subjected to cooling preservation processing.
(6) filling after sterilizing both to obtain this product.
Among the embodiment, used key instrument and equipment are as follows:
Thermostat water bath: DK-98-Π: JMS-240 type；The limited public affairs of PPT-B6000 type electronic balance Foochow China's will scientific instrument
Department；SIGMA 3K15 state China's centrifuge Beijing Five continents east development in science and technology Co., Ltd；High speed Universal pulverizer: the north FW-100
Jing Shi bright Medical Instruments Co., Ltd forever；Nine sun of refiner；SIGMA 3K15 state, Five continents east, China's centrifuge Beijing science and technology
Development Co., Ltd；BCD-570WPCJ refrigerator Hefei Meiling Co. Ltd.；Microplate spectrophotometer Tencan
Infinite M200 type Shanghai Christian Dior Biotechnology Co., Ltd；Autoclave sterilizer: MLS-3750 type, Sanyo's electricity strain formula
Commercial firm；DHP constant incubator Shanghai laboratory apparatus Co., Ltd., Factory；Safe and sound company, aseptic operating platform Su Jing group；Microplate reader:
SM100 Shanghai Yongchuang Medical Instrument Co., Ltd.；It is known that how a variety of materials proportion it is improper if, Jiu Huiji
The earth influences mouthfeel, the appearance of final products, how to determine optimal material proportion, is measured according to following method.
Based on test of many times of the invention, the ratio of each component presses percentage successively are as follows: major ingredient: Loquat-Shaped Cake
Juice 55%, red acid berry wine 15%.
Auxiliary material: arhat juice 10%, honeysuckle juice 5%, mint juice 5%, mixing decoction 5%, pharagmitis rhizoma juice 5%.
Under aforementioned proportion, the product can have certain satisfaction in appearance, color and mouthfeel,
In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, the present invention is carried out
It is further described.
Application effect of the invention is explained in detail below with reference to sensory evaluation and related experiment.
Better invention product in order to obtain designs different technology conditions knot by the key technology in the optimization present invention
The method for closing sensory evaluation determines optimal case study on implementation, and the specific method is as follows: it is trained experienced to choose 15 Majors of Food
Sensory evaluation composition of personnel judges group, to this beverage carry out sensory evaluation, then count color (20%), fragrance (20%),
The total score of tissue morphology (20%), flavour (40%), as sensory evaluation index score, standards of grading following table, table one are commented for sense organ
Price card is quasi-.
(1) measurement of stability
In centrifuge tube, configured drink sample 10ml is accurately added, is then centrifuged 15min in 3000r/min centrifuge again,
Top solution is discarded, bottom precipitation weight is weighed, calculates contents of precipitate using following formula.
Rate of deposition=×100%。
As a result: rate of deposition 5%.
(2) ratio optimization
By L9(34) Orthogonal Experiment and Design requirement, it is 100ml with total dosage that fruit juice dosage, wine juice dosage are added by different proportion
Benchmark the results are shown in Table two.
(3) determination of optimum formula
Fruit juice is the main component for making composite beverage have refrigerant mouthfeel, and honeysuckle juice has light Chinese medicine fragrance, should not add
Excessively, adding excessive bitter taste can be obvious, while can also cover other tastes, and wine juice and mixing decoction addition are proper, adjustable
Tart flavour and bitter taste are saved, keeps mouthfeel more balanced.With arhat juice, mixing decoction, fruit juice, pharagmitis rhizoma juice, mint juice, honeysuckle juice,
The percentage of wine juice additive amount is factor level, is carried out using orthogonal test, factor level table is shown in Table three.
(4) plant bacteriostatic activity test, if Fig. 2 is red acid slurry Siraitia grosvenorii throat-clearing throat-moistening beverage provided in an embodiment of the present invention
Biocidal property lab diagram.In figure :(a) biocidal property experiment one.(b) biocidal property experiment two.It specifically includes:
Test the preparation of extracting solution:
By Schisandra chinensis, dried orange peel, Radix Glycyrrhizae, dandelion, honeysuckle, reed root, peppermint pulverization process, picks new fresh mulberry leaf and be placed in baking oven
Pulverization process is taken out in 80 DEG C of flash bakings.The above raw material weighs 2g respectively and is placed in a beaker, and 10 times of distilled water immersions are added
30min boils 10min, cools down, and filters, then filter residue is added 10 times of water, boils 10min, filters, and 2 filtrates is merged, heating is steamed
It boils to 2ml, it is cooling, lOmin is centrifuged with 4000r/min, obtains clear liquid.It is settled to 2ml, obtains the raw material extracting solution of 1g/ml.By ammonia
Section penicillin is made into human injection's concentration of 0.01 g/ml, and antibiotic control group is made.
The preparation of solid medium:
1000ml beef extract~peptone solid medium (staphylococcus aureus) is prepared, prepares ratio are as follows: beef extract 3.0g
Peptone 10.Og, 15~20g of agar, sodium chloride 5.0g and water 1000mL are adjusted to pH 7.0~7.2, stir up to clarification,
121 DEG C of sterilizing 15min, are down between 70 DEG C and 80 DEG C to temperature, are poured into sterile petri dish while hot, form thickness about after cooling
For the agar plate of 4mm；1000mlLB solid medium is prepared, prepares ratio are as follows: beef extract 3.0g, peptone 10.0g, yeast
Cream 5.0g, sodium chloride 5.0g, glucose 10.0g, 15~20g of agar powder, stirring is until clarification, 121 DEG C of sterilizing 15min, to temperature
Degree is down between 70 DEG C and 80 DEG C, is poured into sterile petri dish while hot, is formed the agar plate that thickness is about 4mm after cooling.
The preparation of fluid nutrient medium:
100ml beef extract-peptone fluid nutrient medium (staphylococcus aureus) is prepared, prepares ratio are as follows: beef extract 0.3g, egg
White peptone 1.0g, sodium chloride 0.5g.Stirring is until clarification, 121 DEG C of sterilizing 15min；100mlLB fluid nutrient medium is prepared, ratio is prepared
Example are as follows: beef extract 0.3g, peptone 1.0g, yeast extract 0.5g, 0.5g sodium chloride, glucose 1.0g.Stirring is up to uniform, and 121
DEG C sterilizing 15min.
The Escherichia coli 4.0ml of the staphylococcus aureus that Spawn incubation takes -80 DEG C of refrigerators to save and 4 DEG C of preservations, connects
Kind is in fluid nutrient medium, 37 DEG C of 150r shaking table culture 12h, using this as mother liquor.
The measurement of bacteriostasis:
Using filter paper enzyme, filter paper is broken into 6mm disk with punch, filter paper is placed on the sample of 1 g/ml after high pressure sterilization
30min is impregnated in solution.Every ware is coated with 0.5 mL bacterium solution with sterile triangle spreading rod after inverted plate.Filter paper drains more after impregnating well
Remaining test solution, is placed on the culture medium for coating bacterium solution with aseptic nipper.Every ware is in symmetrically placed 4 disks of "+" shape, and every kind of bacterium is parallel
Make four wares, be placed in 37 degree, in constant incubator culture for 24 hours, after culture, in graduated scale test media inhibition zone it is straight
Diameter.
Antibiotic is taken to compare: penicillin (0.01 g/mL), by beverage raw material honeysuckle, Schisandra chinensis, Radix Glycyrrhizae, the mulberry of extraction
Leaf, dandelion, dried orange peel, reed root and peppermint extracting solution, respectively with the concentration of 1 g/ml respectively to staphylococcus aureus and large intestine bar
Bacterium carries out filter paper enzyme and measures bacteriostasis, as a result as shown in Table 4.
As shown in Table 4, generally most of material has bacteriostasis to staphylococcus aureus and Escherichia coli, to gold
For staphylococcus aureus, fungistatic effect to weak from putting in order by force are as follows: benzyl penicillin T.GT.GT peppermint > Schisandra chinensis > Radix Glycyrrhizae > gold and silver
Flower > reed root > dandelion > dried orange peel > mulberry leaf.For Escherichia coli, fungistatic effect to weak from putting in order by force are as follows: mould
Plain > Schisandra chinensis > peppermint > dandelion > dried orange peel > honeysuckle > Radix Glycyrrhizae > mulberry leaf > reed root.Total fungistatic effect is from by force to weak sequence arrangement
Are as follows: benzyl penicillin T.GT.GT peppermint > Schisandra chinensis > honeysuckle > Radix Glycyrrhizae > dandelion > dried orange peel > reed root > mulberry leaf.
(5) measurement of minimal inhibitory concentration (MIC)
Using improved liquid diluting tube method, the test tube of 7 sterilizings is taken, the fluid nutrient medium of 1 mL dissolution is added in every pipe, then
It takes 2 mL of sterile test solution of 0.1g/mL that the 1st test tube is added to mix, inhales 2mL the 2nd test tube is added and mix, dilution
To the 6th test tube, this pipe discards 2 mL, and the 7th test tube is as blank control pipe, so that respectively obtaining concentration is 100,50,25,
12.5,6.25,3.125 the concentration gradient of mg/mL.Aseptically every pipe is added 0.05 mL of bacterium solution and mixes, and is placed in 37 degree
Culture in constant incubator take the time that control tube has the significant growth of bacterium as the most suitable time of observation and evaluation MIC, with drug containing
Measuring a pipe that is low and being visible by naked eyes bacterial growth is MIC, as a result as shown in table five, six.
Table six: minimal inhibitory concentration (MIC) of each raw material to Escherichia coli
According to five table six of table it is found that for staphylococcus aureus, the MIC of Schisandra chinensis is 100mg/ml, and the MIC of honeysuckle is
100mg/ml.For Escherichia coli, the MIC of honeysuckle is 25mg/ml, and the MIC of peppermint is 100mg/ml.It is all one group of "+"
Illustrate that the MIC of this raw material is greater than 100mg/ml.As can be seen from the table, staphylococcus aureus is less than to Escherichia coli MIC,
Prove that each raw material is stronger than staphylococcus aureus to the rejection ability of Escherichia coli.
The present invention will be further described below with reference to examples.
Case study on implementation one:
The preparation method of red acid provided in an embodiment of the present invention slurry Siraitia grosvenorii throat-clearing throat-moistening beverage includes:
Step 1 pre-processes raw material:
Major ingredient: selecting fresh Loquat-Shaped Cake, pyrus nivalis, tremella, and 20% lemon juice is added in Loquat-Shaped Cake and 1% salt solution impregnates
10min carries out color protection, and the Loquat-Shaped Cake and pyrus nivalis after color protection are homogenized in the ratio of 1:1, filtering.
Auxiliary material: Siraitia grosvenorii best in quality, honeysuckle, peppermint, reed root, dried orange peel, Schisandra chinensis is selected to be dried, crushed；
Selected material rate are as follows: red acid berry juice 54%, loquat Juice 10%, tremella juice 15%, snow pear juice 10%, arhat juice 2%,
Honeysuckle juice 3%, mint juice 2%, dried orange peel juice 1%, Schisandra chinensis juice 1%, pharagmitis rhizoma juice 2%
Smashed Siraitia grosvenorii, honeysuckle, peppermint, reed root, dried orange peel, Schisandra chinensis are proportionally added into 10 times of water by step 2 respectively
10min is boiled, is filtered, filter residue adds 10 times of water, 60~70 DEG C of extraction 10min, merging filtrate after filtering；
Step 3, will Step 1: the resulting fruit juice of step 2, arhat juice, honeysuckle juice, mint juice, pharagmitis rhizoma juice, dried orange peel juice,
Schisandra chinensis juice carries out 4000r and is centrifuged 15min；
Step 4, by fruit juice, arhat juice, honeysuckle juice, mint juice, pharagmitis rhizoma juice, dried orange peel juice, Schisandra chinensis juice, red acid berry juice
It is compounded by different proportion；
Step 5 will access 0.1% lactobacillus plantarum after the sterilization of the product after allotment, in 30 DEG C of incubators fermentation 48h;.
Step 6,72h~96h saves after-ripening under the conditions of the product after allotment is carried out 4~8 DEG C of low temperature.
Case study on implementation two:
The preparation method of red acid provided in an embodiment of the present invention slurry Siraitia grosvenorii throat-clearing throat-moistening beverage includes:
Step 1 pre-processes raw material:
Major ingredient: selecting fresh Loquat-Shaped Cake, pyrus nivalis, tremella, and 20% lemon juice is added in Loquat-Shaped Cake and 1% salt solution impregnates
10min carries out color protection, and the Loquat-Shaped Cake and pyrus nivalis after color protection are homogenized in the ratio of 1:2, filtering.
Selected material rate are as follows: red acid berry juice 54%, loquat Juice 5%, tremella juice 20%, snow pear juice 10%, arhat juice
3%, honeysuckle juice 3%, mint juice 1%, dried orange peel juice 1%, Schisandra chinensis juice 2%, pharagmitis rhizoma juice 1%
Auxiliary material: Siraitia grosvenorii best in quality, honeysuckle, peppermint, reed root, dried orange peel, Schisandra chinensis is selected to be dried, crushed；
Smashed Siraitia grosvenorii, honeysuckle, peppermint, reed root, dried orange peel, Schisandra chinensis are proportionally added into 10 times of water by step 2 respectively
10min is boiled, is filtered, filter residue adds 10 times of water, 60~70 DEG C of extraction 10min, merging filtrate after filtering；
Step 3, will Step 1: the resulting fruit juice of step 2, arhat juice, honeysuckle juice, mint juice, pharagmitis rhizoma juice, dried orange peel juice,
Schisandra chinensis juice carries out 4000r and is centrifuged 15min；
Step 4, by fruit juice, arhat juice, honeysuckle juice, mint juice, pharagmitis rhizoma juice, dried orange peel juice, Schisandra chinensis juice, red acid berry juice
It is compounded by different proportion；
Step 5 will access 0.1% lactobacillus plantarum after the sterilization of the product after allotment, in 30 DEG C of incubators fermentation 48h;.
Step 6,72h~96h saves after-ripening under the conditions of the product after allotment is carried out 4~8 DEG C of low temperature.
Case study on implementation three:
The preparation method of red acid provided in an embodiment of the present invention slurry Siraitia grosvenorii throat-clearing throat-moistening beverage includes:
Step 1 pre-processes raw material:
Major ingredient: selecting fresh Loquat-Shaped Cake, pyrus nivalis, tremella, and 20% lemon juice is added in Loquat-Shaped Cake and 1% salt solution impregnates
10min carries out color protection, and the Loquat-Shaped Cake and pyrus nivalis after color protection are homogenized in the ratio of 2:3, filtering.
Selected material rate are as follows: red acid berry juice 49%, loquat Juice 8%, tremella juice 20%, snow pear juice 12%, arhat juice
3%, honeysuckle juice 3%, mint juice 1%, dried orange peel juice 1%, Schisandra chinensis juice 2%, pharagmitis rhizoma juice 1%.
Auxiliary material: Siraitia grosvenorii best in quality, honeysuckle, peppermint, reed root, dried orange peel, Schisandra chinensis is selected to be dried, crushed；
Smashed Siraitia grosvenorii, honeysuckle, peppermint, reed root, dried orange peel, Schisandra chinensis are proportionally added into 10 times of water by step 2 respectively
10min is boiled, is filtered, filter residue adds 10 times of water, 60~70 DEG C of extraction 10min, merging filtrate after filtering；
Step 3, will Step 1: the resulting fruit juice of step 2, arhat juice, honeysuckle juice, mint juice, pharagmitis rhizoma juice, dried orange peel juice,
Schisandra chinensis juice carries out 4000r and is centrifuged 15min；
Step 4, by fruit juice, arhat juice, honeysuckle juice, mint juice, pharagmitis rhizoma juice, dried orange peel juice, Schisandra chinensis juice, red acid berry juice
It is compounded by different proportion；
Step 5 will access 0.1% lactobacillus plantarum after the sterilization of the product after allotment, in 30 DEG C of incubators fermentation 48h;.
Step 6,72h~96h saves after-ripening under the conditions of the product after allotment is carried out 4~8 DEG C of low temperature.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in essence of the invention
Made any modifications, equivalent replacements, and improvements etc., should all be included in the protection scope of the present invention within mind and principle.
Claims (9)
1. the throat-clearing throat-moistening beverage that a kind of red acid berry is primary raw material, which is characterized in that the red acid berry is primary raw material
Throat-clearing throat-moistening beverage be made of major ingredient and auxiliary material；
The major ingredient is made of loquat Juice, snow pear juice, tremella juice and red acid berry juice；
The auxiliary material is made of honeysuckle juice, arhat juice, mint juice, Schisandra chinensis juice, dried orange peel juice and pharagmitis rhizoma juice；
The each component percentage of the major ingredient, auxiliary material are as follows: red acid berry juice 50~60%, loquat Juice 8~12%, tremella juice
12~18%, snow pear juice 8~12%, arhat juice 1~3%, honeysuckle juice 2~5%, mint juice 1~3%, dried orange peel juice 0.5~1.5%,
Schisandra chinensis juice 0.5~1.5% and pharagmitis rhizoma juice 1~3%.
2. a kind of red acid berry as described in claim 1 is the preparation method of the throat-clearing throat-moistening beverage of primary raw material, feature exists
In the red acid berry is that the preparation method of the throat-clearing throat-moistening beverage of primary raw material carries out pretreatment of raw material, infusion, filtering, two
Filling after secondary extraction filtering, merging filtrate, ratio optimization, centrifugation, cold fermentation, after-ripening, sterilizing, it is main for obtaining red acid berry
The throat-clearing throat-moistening beverage product of raw material.
3. the preparation method that red acid berry as claimed in claim 2 is the throat-clearing throat-moistening beverage of primary raw material, which is characterized in that
Carrying out pretreated method to raw material includes:
The pretreatment of major ingredient: selecting fresh Loquat-Shaped Cake, pyrus nivalis, tremella, and 20% lemon juice and 2~3% foods are added in Loquat-Shaped Cake
Salting liquid impregnates 10min and carries out color protection, and the Loquat-Shaped Cake and pyrus nivalis after color protection are homogenized in the ratio of 1:1, filtering；
The pretreatment of auxiliary material: Siraitia grosvenorii best in quality, honeysuckle, peppermint, reed root, dried orange peel, Schisandra chinensis is selected to be dried, powder
It is broken.
4. the preparation method that red acid berry as claimed in claim 2 is the throat-clearing throat-moistening beverage of primary raw material, which is characterized in that
Carry out infusion, filtering, secondary extraction are filtered, in merging filtrate step, specifically include: by smashed Siraitia grosvenorii, honeysuckle, thin
Lotus, reed root, dried orange peel, Schisandra chinensis are proportionally added into 10 times of boiling boiling 10min respectively, filter, and filter residue adds 10 times of water, 60~70 DEG C of leachings
Mention 10min, merging filtrate after filtering.
5. the preparation method that red acid berry as claimed in claim 2 is the throat-clearing throat-moistening beverage of primary raw material, which is characterized in that
In progress ratio optimization, centrifugation step, specifically include: by resulting fruit juice, arhat juice, honeysuckle juice, mint juice, reed root
Juice, dried orange peel juice, Schisandra chinensis juice carry out 4000r and are centrifuged 15min；
By fruit juice, arhat juice, honeysuckle juice, mint juice, pharagmitis rhizoma juice, dried orange peel juice, Schisandra chinensis juice, red acid berry juice by not year-on-year
Example is compounded.
6. the preparation method that red acid berry as claimed in claim 2 is the throat-clearing throat-moistening beverage of primary raw material, which is characterized in that
Carry out cold fermentation method include:
0.1%~0.2% lactobacillus plantarum will be accessed after the sterilization of product after compounding, in 30 DEG C of incubators fermentation 48h.
7. the preparation method that red acid berry as claimed in claim 2 is the throat-clearing throat-moistening beverage of primary raw material, which is characterized in that
The method for carrying out after-ripening includes: 72h~96h preservation after-ripening under the conditions of the product after allotment is carried out 4~8 DEG C of low temperature.
8. the preparation method that red acid berry as claimed in claim 4 is the throat-clearing throat-moistening beverage of primary raw material, which is characterized in that
After being crushed in infusion, 30~45min is boiled；
Add 10 times of water, 60~70 DEG C of extraction 10min in leaching process in proportion；
Infusion, the resulting filtrate of extraction merge simultaneously.
9. a kind of red acid berry prepared using the throat-clearing throat-moistening beverage that red acid berry described in claim 1 is primary raw material.
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