CN115806888A - 一种同时生产倍半萜和初级代谢产物的重组微生物及其应用 - Google Patents
一种同时生产倍半萜和初级代谢产物的重组微生物及其应用 Download PDFInfo
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Abstract
本发明提供了一种同时生产倍半萜和初级代谢产物的重组微生物及其应用，所述初级代谢产物包括泛酸、角鲨烯、脂肪酸和维生素B2中的至少一种；所述菌株含有甲羟戊酸途径、β‑法尼烯合成基因和初级代谢产物合成的相关基因；所述甲羟戊酸途径的相关基因包括ERG10、ERG13、tHMG1、ERG12、ERG8、MVD1、IDI1和ERG20基因，拷贝数为ERG10：ERG13：tHMG1：ERG12：ERG8：MVD1：IDI1：ERG20＝2：2：X：2：2：2：2：2，X为≥1的整数；所述β‑法尼烯合成基因包括bFS，序列如SEQ ID NO.1所示。本发明在生产倍半萜化合物的同时实现初级代谢产物高产。
Description
技术领域
本发明涉及生物工程技术领域，特别涉及一种同时生产倍半萜和初级代谢产物的重组微生物及其应用。
背景技术
倍半萜化合物，是一类由三个异戊二烯单元组成的萜类化合物，广泛分布于植物、微生物，昆虫和各种海洋生物中。随着微生物技术的发展，研究人员实现了一些倍半萜化合物在微生物中的高效合成。倍半萜化合物为挥发性产物，其生物合成过程中常采用双向萃取发酵，使得目标产物会积累在胞外侧的油层中，在工业生产过程中收集油层即可实现目标倍半萜化合物的收集，菌液和菌体则会直接废弃，这将会产生较多的工业废物。在产生溶于油相的胞外产物的同时，生产胞内或胞外非脂溶性产物，可以实现合理利用发酵后的菌液和菌体，有利于工业上绿色经济、节能生产。
初级代谢产物是指微生物通过代谢活动所产生的、自身生长和繁殖所必需的物质，如氨基酸、核苷酸、多糖、脂类、维生素、泛酸、角鲨烯和脂肪酸等。通过初级代谢，能使营养物转化为结构物质、具生理活性物质或为生长提供能量，因此初级代谢产物，通常都是机体生存必不可少的物质，只要在这些物质的合成过程的某个环节上发生障碍，轻则引起生长停止，重则导致机体发生突变或死亡，是一种基本代谢类型。
现有技术中同时生产倍半萜和初级代谢产物的微生物，由于可能存在竞争抑制，难以两者同时高产。
因此，如何有必要开发一种同时高产倍半萜和初级代谢产物的重组微生物。
发明内容
本发明目的是提供一种同时生产倍半萜和初级代谢产物的重组微生物及其应用，在生产倍半萜化合物的同时，可以实现初级代谢产物高产。
在本发明的第一方面，提供了一种同时生产倍半萜和初级代谢产物的菌株，所述菌株含有甲羟戊酸途径、部分或全部经过密码子优化的倍半萜的合成基因和初级代谢产物合成的相关基因；所述初级代谢产物包括泛酸、角鲨烯、脂肪酸和维生素B2中的至少一种；所述倍半萜包括β-法尼烯；
所述的甲羟戊酸途径的相关基因包括ERG10、ERG13、tHMG1、ERG12、ERG8、MVD1、IDI1和ERG20基因；所述甲羟戊酸途径中基因的拷贝数为ERG10：ERG13：tHMG1：ERG12：ERG8：MVD1：IDI1：ERG20＝2：2：X：2：2：2：2：2，X为≥1的整数；
所述β-法尼烯合成基因包括bFS，所述的bFS基因为洋甘菊来源的法尼烯合酶基础上包含F11S、M35T、T319S、I434T、I460V突变，其核苷酸序列如SEQ ID NO.1所示。
进一步地，所述泛酸合成的相关基因包括ilv2、ilv6、ilv5、ilv3、Ecm31p、pan5、pan6和PAND基因；所述的PAND基因经过密码子优化，其核苷酸序列如SEQ ID NO.2所示。
进一步地，所述同时生产倍半萜和所述泛酸的菌株包括：
将质粒pPT1、pPT2和pXZ162共转化至酿酒酵母JVA139获得的菌株JFS1；
或者将所述菌株JFS1经过GAL80基因的敲除，获得的菌株JFS2；
或者将质粒pPT1、pPT3和pXZ162共转化至酿酒酵母JVA139获得的菌株JFS3；
或者将所述菌株JFS3经过GAL80基因的敲除，获得的菌株JFS4；
其中，
所述质粒pPT1为以pMD19T为骨架载体，启动子为pGAL1、pGAL2、pGAL7和pGAL10，包含ilv2、ilv6、ilv5和ilv3基因，所述质粒pPT1的核苷酸序列如SEQ ID NO.3所示；
所述质粒pPT2为以pMD19T为骨架载体，启动子为pGAL1、pGAL2、pGAL7和pGAL10，包含Ecm31p、pan5、pan6和PAND基因，所述质粒pPT2的核苷酸序列如SEQ ID NO.4所示；
所述质粒pPT3为以pMD19T为骨架载体，启动子为pGAL1、pGAL7和pGAL10，包含Ecm31p、pan5和pan6基因，所述质粒pPT3的核苷酸序列如SEQ ID NO.5所示；
所述质粒pXZ162的核苷酸序列如SEQ ID NO.6所示。
进一步地，所述角鲨烯合成的相关基因包括POS5、ADH2、ACS2、ALD6和ERG9基因。
进一步地，所述同时生产倍半萜和所述角鲨烯的菌株包括：
将质粒pXZ171、pXZ159线性化后和pXZ161共转化至酿酒酵母JVA139获得的菌株JSX1；后将质粒pSX01线性化后和pXZ162共转化至所述菌株JSX1获得的菌株JSX2；
或者将所述菌株JSX2经过GAL80基因的敲除，获得的菌株JSX3；
其中，
所述质粒pXZ171以pMD19T为骨架载体，启动子为pGAL1、pGAL7和pGAL10，包含ADH2、ACS2和ALD6基因，所述质粒pXZ171的核苷酸序列如SEQ ID NO.7所示；
所述质粒pXZ159以pMD19T为骨架载体，启动子为pGAL1，包含POS5基因，所述质粒pXZ159的核苷酸序列如SEQ ID NO.8所示；
所述质粒pSX01以pMD19T为骨架载体，启动子为pHXT1，包含ERG9基因，所述质粒pSX01的核苷酸序列如SEQ ID NO.9所示；
所述质粒pXZ161的核苷酸序列如SEQ ID NO.10所示。
进一步地，所述脂肪酸合成的相关基因包括ACC1、FAS1和FAS2基因，并采用葡萄糖诱导型启动子pHXT1和大肠杆菌来源的截短信号肽的硫解酶tesA。
进一步地，所述同时生产倍半萜和所述脂肪酸的菌株包括：
将质粒pZF01、pZF02和pZF03分别线性化后和pZF04共转化至酿酒酵母JVA139获得的菌株JZF01；
或者将所述菌株JZF01经过GAL80基因的敲除，获得的菌株JZF02；
其中，
所述质粒pZF01以pMD19T为骨架载体，启动子为pHXT1，包含tesA基因，所述质粒pZF01的核苷酸序列如SEQ ID NO.11所示；
所述质粒pZF02以pMD19T为骨架载体，启动子为pHXT1，包含ACC1基因，所述质粒pZF02的核苷酸序列如SEQ ID NO.12所示；
所述质粒pZF03以pMD19T为骨架载体，启动子为pHXT1，包含FAS1和FAS2基因，所述质粒pZF03的核苷酸序列如SEQ ID NO.13所示；
所述质粒pZF04的核苷酸序列如SEQ ID NO.14所示。
进一步地，所述维生素B2合成的相关基因包括RIB1、RIB7、RIB2、RIB3、RIB4和RIB5基因。
进一步地，所述同时生产倍半萜和所述维生素B2的菌株包括：
将质粒pVB1和pVB2分别线性化后和pXZ162共转化至酿酒酵母JVA139获得的菌株JVB01；
或者将所述菌株JVB01经过GAL80基因的敲除，获得的菌株JVB02；
其中，
所述质粒pVB1以pMD19T为骨架载体，启动子为pGAL1、pGAL7和pGAL10，包含RIB1、RIB7和RIB2基因，所述质粒pVB1的核苷酸序列如SEQ ID NO.15所示；
所述质粒pVB2以pMD19T为骨架载体，启动子为pGAL1、pGAL7和pGAL10，包含RIB3、RIB4和RIB5基因，所述质粒pVB2的核苷酸序列如SEQ ID NO.16所示；
所述质粒pXZ162的核苷酸序列如SEQ ID NO.6所示。
在本发明的第二方面，提供了所述的同时生产倍半萜和初级代谢产物的菌在生产倍半萜和初级代谢产物中的应用。将所述的菌株种子液接入培养基中进行真核表达得到倍半萜和初级代谢产物。
本发明实施例中的一个或多个技术方案，至少具有如下技术效果或优点：
本发明提供的一种同时生产倍半萜和初级代谢产物的重组微生物及其应用，在生产倍半萜化合物的同时，可以实现初级代谢产物高产的重组微生物。其中，重组微生物加强了甲羟戊酸途径，甲羟戊酸途径中基因的拷贝数为ERG10：ERG13：tHMG1：ERG12：ERG8：MVD1：IDI1：ERG20＝2：2：X：2：2：2：2：2，X为大于等于1的整数。其中，ERG10为编码乙酰乙酰辅酶A硫解酶的基因，ERG13为编码HMG-CoA合酶的基因，tHMG1为编码HMG-CoA还原酶的基因，ERG12为编码甲羟戊酸激酶的基因，ERG8为编码甲羟戊酸-5-磷酸激酶的基因，MVD1为编码甲羟戊酸焦磷酸脱羧酶的基因，IDI1为编码异戊二烯焦磷酸异构酶的基因，ERG20为编码法尼烯焦磷酸合酶的基因。在菌株中导入法尼烯合成基因bFS实现法尼烯的高产。在高产法尼烯的菌株基础上，进一步加强菌株初级代谢产物角鲨烯或维生素B2或泛酸或脂肪酸的高产。
附图说明
为了更清楚地说明本发明实施例中的技术方案，下面将对实施例描述中所需要使用的附图作一简单地介绍，显而易见地，下面描述中的附图是本发明的一些实施例，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其它的附图。
图1为质粒pPT1的图谱；
图2为质粒pPT2的图谱；
图3为质粒pPT3的图谱；
图4为质粒pXZ162的图谱；
图5为质粒pXZ171的图谱；
图6为质粒pXZ159的图谱；
图7为质粒pXZ161的图谱；
图8为质粒pSX01的图谱；
图9为质粒pZF01的图谱；
图10为质粒pZF02的图谱；
图11为质粒pZF03的图谱；
图12为质粒pZF04的图谱；
图13为质粒pVB1的图谱；
图14为质粒pVB2的图谱；
图15为质粒pZY600的图谱；
图16为质粒pZY413的图谱；
图17为质粒pZY412L的图谱；
图18为质粒pZY414的图谱；
图19为质粒pZY900的图谱；
图20为质粒pBFS45的图谱。
具体实施方式
下文将结合具体实施方式和实施例，具体阐述本发明，本发明的优点和各种效果将由此更加清楚地呈现。本领域技术人员应理解，这些具体实施方式和实施例是用于说明本发明，而非限制本发明。
在整个说明书中，除非另有特别说明，本文使用的术语应理解为如本领域中通常所使用的含义。因此，除非另有定义，本文使用的所有技术和科学术语具有与本发明所属领域技术人员的一般理解相同的含义。若存在矛盾，本说明书优先。
除非另有特别说明，本发明中用到的各种原材料、试剂、仪器和设备等，均可通过市场购买获得或者可通过现有方法获得。
本申请实施例的技术方案为解决上述技术问题，总体思路如下：
根据本发明实施例一种典型的实施方式，提供同时生产倍半萜和初级代谢产物的菌株，
所述菌株含有甲羟戊酸途径的相关基因、部分或全部经过密码子优化的倍半萜的合成基因和初级代谢产物合成的相关基因；所述初级代谢产物包括泛酸、角鲨烯、脂肪酸和维生素B2中的至少一种；所述倍半萜包括β-法尼烯；
所述的甲羟戊酸途径的相关基因包括ERG10、ERG13、tHMG1、ERG12、ERG8、MVD1、IDI1和ERG20基因；所述甲羟戊酸途径中基因的拷贝数为ERG10：ERG13：tHMG1：ERG12：ERG8：MVD1：IDI1：ERG20＝2：2：X：2：2：2：2：2，X为≥1的整数；
所述β-法尼烯合成基因包括bFS，所述的bFS基因为洋甘菊来源的法尼烯合酶基础上包含F11S、M35T、T319S、I434T、I460V突变，其核苷酸序列如SEQ ID NO.1所示。
所述ERG10、ERG13、tHMG1、ERG12、ERG8、MVD1、IDI1、ERG20在NCBI中的Accession/GENE id见下表1。
表1-甲羟戊酸途径的相关基因
| 基因 | 全称 | Accession/GENE id |
| ERG10 | 乙酰乙酰辅酶A硫解酶的基因 | 856079 |
| ERG13 | HMG-CoA合酶的基因 | 854913 |
| tHMG1 | HMG-CoA还原酶的基因 | 854900，截去4-1659bp |
| ERG12 | 甲羟戊酸激酶的基因 | NM_001182715.1 |
| ERG8 | 甲羟戊酸-5-磷酸激酶的基因 | CP046093.1，689693..691048 |
| MVD1 | 甲羟戊酸焦磷酸脱羧酶的基因 | NM_001183220.1 |
| IDI1 | 异戊二烯焦磷酸异构酶的基因 | NM_001183931.1 |
| ERG20 | 法尼烯焦磷酸合酶的基因 | 853272 |
本发明实施例的重组微生物加强了甲羟戊酸途径，甲羟戊酸途径中基因的拷贝数为ERG10：ERG13：tHMG1：ERG12：ERG8：MVD1：IDI1：ERG20＝2：2：X：2：2：2：2：2，X为大于等于1的整数。其中，ERG10为编码乙酰乙酰辅酶A硫解酶的基因，ERG13为编码HMG-CoA合酶的基因，tHMG1为编码HMG-CoA还原酶的基因，ERG12为编码甲羟戊酸激酶的基因，ERG8为编码甲羟戊酸-5-磷酸激酶的基因，MVD1为编码甲羟戊酸焦磷酸脱羧酶的基因，IDI1为编码异戊二烯焦磷酸异构酶的基因，ERG20为编码法尼烯焦磷酸合酶的基因。
上述ERG10：ERG13：tHMG1：ERG12：ERG8：MVD1：IDI1：ERG20＝2：2：X：2：2：2：2：2，X为大于等于1的整数的原因：上述拷贝数的比例能够加强甲羟戊酸途径，使得最大限度的提供前体供应；
在菌株中导入法尼烯合成基因bFS，所述的bFS基因为洋甘菊来源的法尼烯合酶基础上包含F11S、M35T、T319S、I434T、I460V突变，其核苷酸序列如SEQ ID NO.1所示，突变后能够实现法尼烯的高产。
同时在所述高产法尼烯的菌株基础上，进一步加强菌株初级代谢产物角泛酸、角鲨烯、脂肪酸和维生素B2中的至少一种的高产。
作为一种具体的实施方式，所述同时生产倍半萜和初级代谢产物的菌株为同时生产倍半萜和所述泛酸的菌株；其中，
所述泛酸合成的相关基因包括ilv2、ilv6、ilv5、ilv3、Ecm31p、pan5、pan6和PAND基因；所述的PAND基因经过密码子优化，其核苷酸序列如SEQ ID NO.2所示。
所述同时生产倍半萜和所述泛酸的菌株包括：
将质粒pPT1、pPT2和pXZ162共转化至酿酒酵母JVA139获得的菌株JFS1；
或者将所述菌株JFS1经过GAL80基因的敲除，获得的菌株JFS2；
或者将质粒pPT1、pPT3和pXZ162共转化至酿酒酵母JVA139获得的菌株JFS3；
或者将所述菌株JFS3经过GAL80基因的敲除，获得的菌株JFS4；
其中，
如图1所示，所述质粒pPT1为以pMD19T为骨架载体，启动子为pGAL1、pGAL2、pGAL7和pGAL10，包含ilv2、ilv6、ilv5和ilv3基因，所述质粒pPT1的核苷酸序列如SEQ ID NO.3所示；
如图2所示，所述质粒pPT2为以pMD19T为骨架载体，启动子为pGAL1、pGAL2、pGAL7和pGAL10，包含Ecm31p、pan5、pan6和PAND基因，所述质粒pPT2的核苷酸序列如SEQ ID NO.4所示；
如图3所示，所述质粒pPT3为以pMD19T为骨架载体、启动子为pGAL1、pGAL7、pGAL10、包含Ecm31p、pan5和pan6基因，所述质粒pPT3的核苷酸序列如SEQ ID NO.5所示；
如图4所示，所述质粒pXZ162的核苷酸序列如SEQ ID NO.6所示。所述质粒pXZ162为靶向rDNA位点的gRNA质粒，用于引导Cas9蛋白切割靶向位点，实现目标基因向目标位点的整合。
表2-泛酸合成的相关基因
上述技术方案中，菌株JFS1、JFS2、JFS3和JFS4能够生产泛酸的原理和能够高产的原因：在野生型酿酒酵母中，其为泛酸缺陷菌株，需要补充泛酸以维持其正常生长，目前还未见报道在酿酒酵母中实现泛酸的高效合成；本发明通过过表达泛酸合成途径的限速酶(具体见表2)以及引入异源的大肠杆菌来源的经过酿酒酵母密码子优化后合成的基因天冬氨酸-1-脱羧酶(Aspartate 1-decarboxylase，PAND)以提供充足的前体，可以实现在酿酒酵母菌株中泛酸的高效合成。
其中，菌株JFS2和JFS4由于敲除了其GAL80基因，敲除Gal80后，菌株可以实现在不添加半乳糖诱导的情况下合成倍半萜和高产目标初级代谢产物，可以减少实验流程，减小发酵成本。
作为一种具体的实施方式，所述同时生产倍半萜和初级代谢产物的菌株为同时生产倍半萜和所述角鲨烯的菌株；其中，
所述角鲨烯合成的相关基因包括POS5、ADH2、ACS2、ALD6和ERG9基因。
所述同时生产倍半萜和所述角鲨烯的菌株包括：
将质粒pXZ171、pXZ159线性化后和pXZ161共转化至酿酒酵母JVA139获得的菌株JSX1；后将质粒pSX01线性化后和pXZ162共转化至所述菌株JSX1获得的菌株JSX2；
或者将所述菌株JSX2经过GAL80基因的敲除，获得的菌株JSX3；
其中，
如图5所示，所述质粒pXZ171以pMD19T为骨架载体，启动子为pGAL1、pGAL7和pGAL10，包含ADH2、ACS2和ALD6基因，所述质粒pXZ171的核苷酸序列如SEQ ID NO.7所示；
如图6所示，所述质粒pXZ159以pMD19T为骨架载体，启动子为pGAL1，包含POS5基因，所述质粒pXZ159的核苷酸序列如SEQ ID NO.8所示；
如图8所示，所述质粒pSX01以pMD19T为骨架载体，启动子为pHXT1，包含ERG9基因，所述质粒pSX01的核苷酸序列如SEQ ID NO.9所示；
如图7所示，所述质粒pXZ161的核苷酸序列如SEQ ID NO.10所示。所述质粒pXZ161为靶向DPP1和exg1位点的gRNA质粒，用于引导Cas9蛋白切割靶向位点，实现目标基因向目标位点的整合。
表3-角鲨烯合成的相关基因
| 基因 | 全称 | Accession/GENE id |
| POS5 | NADH激酶 | 855913 |
| ADH2 | 乙醇脱氢酶 | 855349 |
| ACS2 | 乙酰辅酶A合成酶 | 850846 |
| ALD6 | 乙醛脱氢酶 | 856044 |
| ERG9 | 角鲨烯合酶 | 856597 |
上述技术方案中，菌株JSX2和JSX3能够生产角鲨烯的原理和能够高产的原因：在高效合成法尼烯的菌株中，会竞争角鲨烯的合成前体FPP，因此难以实现两种化合物的同时高效合成，在本发明中，进一步加强MVA途径共同前体乙酰辅酶A的供应，使用诱导型启动子pHXT1控制ERG9的过表达，通过时空交错，在前期高效合成角鲨烯，后期高效合成法尼烯，从而可以实现两种化合物的高效合成；
其中，菌株JSX3由于敲除了其GAL80基因，敲除Gal80后，菌株可以实现在不添加半乳糖诱导的情况下合成倍半萜和高产目标初级代谢产物，可以减少实验流程，减小发酵成本。
作为一种具体的实施方式，所述同时生产倍半萜和初级代谢产物的菌株为同时生产倍半萜和所述脂肪酸的菌株，其中，
所述脂肪酸合成的相关基因包括ACC1、FAS1和FAS2基因，并采用葡萄糖诱导型启动子pHXT1和大肠杆菌来源的截短信号肽的硫解酶tesA。
所述同时生产倍半萜和所述脂肪酸的菌株包括：
将质粒pZF01、pZF02和pZF03分别线性化后和pZF04共转化至酿酒酵母JVA139获得的菌株JZF01；
或者将所述菌株JZF01经过GAL80基因的敲除，获得的菌株JZF02；
其中，
如图9所示，所述质粒pZF01以pMD19T为骨架载体，启动子为pHXT1，包含tesA基因，所述质粒pZF01的核苷酸序列如SEQ ID NO.11所示；
如图10所示，所述质粒pZF02以pMD19T为骨架载体，启动子为pHXT1，包含ACC1基因，所述质粒pZF02的核苷酸序列如SEQ ID NO.12所示；
如图11所示，所述质粒pZF03以pMD19T为骨架载体，启动子为pHXT1，包含FAS1和FAS2基因，所述质粒pZF03的核苷酸序列如SEQ ID NO.13所示；
如图12所示，所述质粒pZF04的核苷酸序列如SEQ ID NO.14所示。所述质粒pZF04为靶向POX1、FAA1、FAA4位点的gRNA质粒，用于引导Cas9蛋白切割靶向位点，实现目标基因向目标位点的整合。
表4-脂肪酸合成的相关基因
上述技术方案中，菌株JZF01和JZF02能够生产脂肪酸的原理和能够高产的原因：在高效合成法尼烯的菌株中，会竞争脂肪酸的合成前体乙酰辅酶A，因此难以实现两种化合物的同时高效合成，在本发明中，使用诱导型启动子pHXT1控制脂肪酸合成基因(如表4中的ACC1、FAS1和FAS2)的过表达，通过时空交错，在前期高效合成脂肪酸，后期高效合成法尼烯，从而可以实现两种化合物的高效合成；同时，通过质粒pZF04敲除脂肪酸降解相关基因酰基CoA合成酶FAA1，FAA4，β-氧化酶POX1进一步提高脂肪酸的产量；
其中，菌株JZF02由于敲除了其GAL80基因，敲除Gal80后，菌株可以实现在不添加半乳糖诱导的情况下合成倍半萜和高产目标初级代谢产物，可以减少实验流程，减小发酵成本。
作为一种具体的实施方式，所述同时生产倍半萜和初级代谢产物的菌株为同时生产倍半萜和所述维生素B2的菌株，其中，
所述维生素B2合成的相关基因包括RIB1、RIB7、RIB2、RIB3、RIB4和RIB5基因。
所述同时生产倍半萜和所述维生素B2的菌株包括：
将质粒pVB1和pVB2分别线性化后和pXZ162共转化至酿酒酵母JVA139获得的菌株JVB01；
或者将所述菌株JVB01经过GAL80基因的敲除，获得的菌株JVB02；
其中，
如图13所示，所述质粒pVB1以pMD19T为骨架载体，启动子为pGAL1、pGAL7和pGAL10，包含RIB1、RIB7和RIB2基因，所述质粒pVB1的核苷酸序列如SEQ ID NO.15所示；
如图14所示，所述质粒pVB2以pMD19T为骨架载体，启动子为pGAL1、pGAL7和pGAL10，包含RIB3、RIB4和RIB5基因，所述质粒pVB2的核苷酸序列如SEQ ID NO.16所示；
如图4所示，所述质粒pXZ162的核苷酸序列如SEQ ID NO.6所示。所述质粒pXZ162为靶向rDNA位点的gRNA质粒，用于引导Cas9蛋白切割靶向位点，实现目标基因向目标位点的整合。
表5-维生素B2合成的相关基因
上述技术方案中，菌株JVB01和JVB02能够生产维生素B2的原理和能够高产的原因：目前还未见报道在酿酒酵母中实现维生素B2的高效合成。本发明在此通过过表达维生素B2合成途径的限速酶(如表5所示)，在高效合成法尼烯的菌株基础上可以实现维生素B2的高效合成。
其中，菌株JVB02由于敲除了其GAL80基因，敲除Gal80后，菌株可以实现在不添加半乳糖诱导的情况下合成倍半萜和高产目标初级代谢产物，可以减少实验流程，减小发酵成本。
在其他实施方式中，所述初级代谢产物包括泛酸、角鲨烯、脂肪酸和维生素B2中的2-4种，同时生产倍半萜、泛酸、角鲨烯、脂肪酸和维生素B2的微生物也可包含在本发明的保护范围内。
下面将结合实施例及实验数据对本申请的一种同时生产倍半萜和初级代谢产物的重组微生物及其应用进行详细说明。
实施例1、底盘酵母菌株所需载体构建
(1)质粒pZY600
质粒pZY600的作用是整合Cas9蛋白，以便后续使用Crispr-cas9基因编辑技术进行菌株改造。质粒pZY600的相关特征：△ChrXII-2：Hygr_pTEF1_Cas9_tCYC1(图15所示)，其中，pTEF1_Cas9_tCYC1指的是表达TEF1启动子控制表达的Cas9，终止子为cyc1，筛选标记为Hygr即潮霉素；ΔchrXII-2指的是该表达框插入到的染色体的位置。
质粒pZY600的具体构建过程：(1)以质粒pZY403(所述质粒pZY403的构建过程见下方)为模板，用引物G6001-F和G6001-R通过PCR扩增得到片段G6001(用于整合Cas9基因的ChrXII-2位点同源左、右臂，引入NotI酶切位点，含有氨苄抗性与ura标签的质粒骨架)；(2)以pZY403为模板，用引物G6002-F和G6002-R通过PCR扩增得到片段G6002(潮霉素抗性基因及其启动子TEF终止子TEF)；(3)以p43802(addgene Plasmid#43802)为模板，用引物G6003-F和G6003-R通过PCR扩增得到片段G6003(基因Cas9及其启动子TEF1终止子CYC1)。(3)通过DNA assemble(酵母组装)的方法将以上片段在酿酒酵母体内重组构建pZY600，然后在大肠杆菌内扩增，酶切验证以及测序正确后，得到pZY600。NotI酶切线性化后得到带有目的基因的片段，其序列如SEQ ID NO.17所示。
其中，所述质粒pZY403的构建为：(1)以酵母30000B基因组为模板，用引物Z4031-F和Z4031-R通过PCR扩增得到片段Z4031(用于整合ERG13基因的ChrXII-2位点同源左臂)；(2)以酵母30000B基因组为模板，用引物Z4032-F和Z4032-R通过PCR扩增得到片段Z4032(启动子)；(3)以酵母30000B基因组为模板，用引物Z4033-F和Z4033-R通过PCR扩增得到片段Z4033(ERG13基因)；(4)以酵母30000B基因组为模板，用引物Z4034-F和Z4034-R通过PCR扩增得到片段Z4034(终止子)；(5)以质粒pZY402(详见CN 111019850 A)为模板，用引物Z4035-F和Z4035-R通过PCR扩增得到片段Z4035(筛选标记)；(6)以酵母30000B基因组为模板，用引物Z4036-F和Z4036-R通过PCR扩增得到片段Z4036(用于整合ERG13基因的ChrXII-2位点同源右臂)；(7)以pZY402(详见CN 111019850 A)为模板，用引物Z4037-F和Z4037-R通过PCR扩增得到片段Z4037(引入NotI酶切位点，含有氨苄抗性与ura标签的质粒骨架)；(8)通过DNA assemble(酵母组装)的方法将以上片段在酿酒酵母体内重组构建pZY403，然后在大肠杆菌内扩增，酶切验证以及测序正确后，得到pZY403。
构建上述质粒所用各引物序列见表6：
表6-质粒pZY600构建过程中所需引物
(2)质粒pZY413和gRNA质粒pZY607
质粒pZY413的作用是过表达MVA途径中的相关基因，为后续进行基因筛选提供一个高效的FPP前体合成底盘菌株。质粒pZY413的相关特征：△ChrXI-3：ERG8_pGAL1pGAL10_tHMG1_pGAL7_ERG12(图16)，用启动子GAL1、GAL7、GAL10分别控制表达基因ERG8、ERG12、THMG1，插入的染色体位点为ChrXI-3。
质粒pZY413的具体构建过程：(1)以质粒pZY402(详见CN 111019850 A)为模板，用引物4131-F和4121-R通过PCR扩增得到片段4131(用于整合ERG8、tHMG1、ERG12基因的同源右臂，引入NotI酶切位点以及含有氨苄抗性与ura标签的质粒骨架的一部分)；(2)以pZY402(详见CN 111019850 A)为模板，用引物4122-F和4132-R通过PCR扩增得到片段4132(含有氨苄抗性与ura标签的质粒骨架的剩余部分，用于整合ERG8、tHMG1、ERG12基因的ChrXI-3位点同源右臂的一部分，引入NotI酶切位点)；(3)以pZY402为模板，用引物4133-F和4133-R通过PCR扩增得到片段4133(用于整合ERG8、tHMG1、ERG12基因的ChrXI-3位点同源右臂的剩余部分，目的基因ERG8、tHMG1、ERG12及其启动子终止子的一部分)；(4)以pZY402为模板，用引物4134-F和4134-R通过PCR扩增得到片段4134(目的基因ERG8、tHMG1、ERG12及其启动子终止子的剩余部分)。(5)通过DNA assemble的方法将以上片段在酿酒酵母体内重组构建pZY413，然后在大肠杆菌内扩增，酶切验证以及测序正确后，得到pZY413。NotI酶切线性化后得到带有目的基因的片段，其序列如SEQ ID NO.18所示。
gRNA质粒pZY607是靶向ChrXI-3位点的gRNA质粒，用于Crispr-Cas9基因编辑技术中靶点识别切割酿酒酵母基因组DNA的工具质粒，其在质粒p43803(http://www.addgene.org/43803/)的基础上用atatgtctctaattttggaa替换了guide-RNA序列。具体构建过程为：(1)以质粒p43803为模板，用引物6071-F和G6031-R通过PCR扩增得到片段6071(用于靶向识别的20bp靶点序列、gRNAscafold、终止子SUP4以及含有氨苄抗性与ura标签的质粒骨架的一部分)；(2)以p43803为模板，用引物G6032-F和6072-R通过PCR扩增得到片段6072(含有氨苄抗性与ura标签的质粒骨架的剩余部分，用于靶向识别的20bp靶点序列以及启动子SNR52)。(3)通过DNAassemble的方法将以上片段在酿酒酵母体内重组构建pZY607。
构建上述质粒所用各引物序列见下表7：
表7-质粒pZY413和gRNA质粒pZY607构建过程所需引物
(3)质粒pZY412L和gRNA质粒pZY606
质粒pZY412L的作用是过表达MVA途径中的相关基因，为后续进行基因筛选提供一个高效的FPP前体合成底盘菌株。质粒pZY412L的相关特征：△ChrXII-4：IDI1_pGAL1pGAL10_ERG10_pGAL7_MVD1(图17)，用启动子GAL1、GAL7、GAL10分别控制表达基因IDI1、MVD1、ERG10，插入的染色体位点为ChrXII-4。
所述质粒pZY412L的具体构建过程：(1)用引物412-F和412-R通过PCR无模板变性退火得到片段pZY412 SPD(一段无意义的DNA序列，用于替换pZY410(详见CN 111019850 A)中的g418抗性基因，含有SalI和SpeI酶切位点的粘性末端)，此无模板变性退火PCR的反应体系为10μL，其中引物分别为4.5μL，10×T4 DNA ligase buffer为1μL，设置以下PCR程序：95℃，5min；降落PCR，95℃，1min，-1℃/cycle，70cycle；25℃，5min；12℃，∞(该片段经无模板变性退火PCR后取出可于-40℃暂存)。(2)用SalI和SpeI酶切pZY401(详见CN 111019850A)后胶回收获得12835bp的大片段pZY412 VPD(含有目的基因MVD1、ERG10、IDI1以及氨苄抗性与ura筛选标记的载体片段)。(3)将片段pZY412 SPD与片段pZY412 VPD按照摩尔比3:1的比例用T4 DNA连接酶在16℃条件下过夜酶连(约20h)，酶连产物转入大肠杆菌DH10B感受态细胞扩增，酶切验证以及测序正确后，得到pZY412L的质粒。NotI酶切线性化后得到带有目的基因的片段，其序列如SEQ ID NO.19所示。
gRNA质粒pZY606是靶向Chr XII-4位点的gRNA质粒，用于Crispr-Cas9基因编辑技术中靶点识别切割酿酒酵母基因组DNA的工具质粒，其在质粒p43803(http://www.addgene.org/43803/)的基础上用gcttcaagaattgagtaaac替换了guide-RNA序列。具体构建过程为：(1)以质粒p43803为模板，用引物6061-F和G6031-R通过PCR扩增得到片段6061(用于靶向识别的20bp靶点序列、gRNA scafold、终止子SUP4以及含有氨苄抗性与ura标签的质粒骨架的一部分)；(2)以p43803为模板，用引物G6032-F和6062-R通过PCR扩增得到片段6062(含有氨苄抗性与ura标签的质粒骨架的剩余部分，用于靶向识别的20bp靶点序列以及启动子SNR52)。(3)通过DNA assemble的方法将以上片段在酿酒酵母体内重组构建pZY606。
构建上述质粒所用各引物序列见下表8：
表8-质粒pZY412L和gRNA质粒pZY606构建过程所需引物
(4)质粒pZY414和gRNA质粒pZY608
质粒pZY414的作用是过表达MVA途径中的相关基因，为后续进行基因筛选提供一个高效的FPP前体合成底盘菌株。质粒pZY414的相关特征：△ChrX-3：pGAL1_ERG13_pGAL10_tHMG1(图18)，用启动子GAL1、GAL10分别控制表达基因ERG13、THMG1，插入的染色体位点为ChrX-3。
所述质粒pZY414的具体构建过程：(1)以酿酒酵母菌株CEN.PK2-1D(30000B)基因组为模板，用引物4141-F和4141-R通过PCR扩增得到片段4141(用于整合ERG13、tHMG1基因的ChrX-3位点同源右臂，引入NotI酶切位点)；(2)以质粒pRS426(ATCC87333)为模板，用引物4142-F和4142-R通过PCR扩增得到片段4142(含有氨苄抗性与ura标签的质粒骨架)；(3)以30000B基因组为模板，用引物4143-F和4143-R通过PCR扩增得到片段4143(用于整合ERG13、tHMG1基因的ChrX-3位点同源右臂，引入NotI酶切位点)；(4)以pZY410(详见CN111019850 A)为模板，用引物4144-F和4144-R通过PCR扩增得到片段4144(目的基因ERG13、tHMG1及其启动子终止子)。(4)通过DNA assemble的方法将以上片段在酿酒酵母体内重组构建pZY414，然后在大肠杆菌内扩增，酶切验证以及测序正确后，得到pZY414的质粒。NotI酶切线性化后得到带有目的基因的片段，其序列如SEQ ID NO.20所示。
所述gRNA质粒pZY608是靶向ChrX-3位点的gRNA质粒，用于Crispr-Cas9基因编辑技术中靶点识别切割酿酒酵母基因组DNA的工具质粒，其在质粒p43803的基础上用ctaatgtgtccgcgtttcta替换了guide-RNA序列。具体构建过程为：(1)以质粒p43803(http://www.addgene.org/43803/)为模板，用引物6081-F和G6031-R通过PCR扩增得到片段6081(用于靶向识别的20bp靶点序列、gRNA scafold、终止子SUP4以及含有氨苄抗性与ura标签的质粒骨架的一部分)；(2)以p43803为模板，用引物G6032-F和6082-R通过PCR扩增得到片段6082(含有氨苄抗性与ura标签的质粒骨架的剩余部分，用于靶向识别的20bp靶点序列以及启动子SNR52)。(3)通过DNA assemble的方法将以上片段在酿酒酵母体内重组构建pZY608，然后在大肠杆菌内扩增，酶切验证以及测序正确后，得到pZY608。
构建上述质粒所用各引物序列见下表9：
表9-质粒pZY414和gRNA质粒pZY608构建过程所需引物
实施例2、底盘酵母菌株构建
1、将实施例1获得的质粒pZY600线性化后片段转入酿酒酵母菌株(可以为包含CEN.PK2-1D，CEN.PK2-1C，BY4742，BY4741，CEN.PK113-5D等酿酒酵母菌株，本发明实施例具体选用CEN.PK2-1D)，根据同源重组来整合到染色体上，其整合位点为ChrXII-2，筛选标记为潮霉素(Hygr)，至此成功构建背景菌株酿酒酵母JCR1。
2、将实施例1获得的质粒pZY413线性化后片段，与实施例1获得的gRNA质粒pZY607一同转化至所述酿酒酵母JCR1，筛选标记为尿嘧啶(URA3)，5-FOA反筛去除质粒后获得酿酒酵母JCR23。
3、将实施例1获得的质粒pZY414线性化后片段，与实施例1获得的gRNA质粒pZY608一同转化至所述酿酒酵母JCR23，筛选标记为尿嘧啶(URA3)，5-FOA反筛去除质粒后获得酿酒酵母JCR25。
4、将实施例1获得的质粒pZY412L线性化后片段，与实施例1获得的gRNA质粒pZY606一同转化至所述酿酒酵母JCR25，筛选标记为尿嘧啶(URA3)，5-FOA反筛去除质粒后获得酿酒酵母菌株JCR27。
表10
实施例3、法尼烯合酶的表达载体的构建
1、质粒pZY900
质粒pZY900的相关特征：△LEU2：LEU2(URA3)_TCYC1_LacZ_pGAL10pGAL1_ERG20_tERG20(图19)，用启动子GAL1、GAL10分别控制表达基因ERG20、LacZ，筛选标记为Leu2，插入的染色体位点为Leu2。
所述质粒pZY900具体构建过程：(1)以酵母S288c基因组为模板，900-1F/1R、900-2F/2R、900-6F/6R、900-7F/7R分别扩增获得片段9001(Leu2的左同源臂)、9002(终止子tTDH2)、9006(基因ERG20与终止子tERG20)、9007(Leu2右臂)；(2)以酵母30000B的基因组为模板，用引物900-3F/3R、900-5F/5R分别扩增获得片段9003(终止子tCYC1)和9005(启动子pGAL1和Pgal10)；(3)以引物900-8F/8R扩增获得质粒骨架(引入MssI酶切位点，筛选标记)。(4)通过DNA assemble(酵母组装)的方法将以上片段在酿酒酵母体内重组构建pZY900，然后在大肠杆菌内扩增，酶切验证以及测序正确后，得到pZY900，序列如SEQ ID NO.11所示。构建上述质粒所用各引物序列见下表11：
表11-质粒pZY900构建所需引物
2、质粒pBFS45
按照酿酒酵母密码子优化的含F11S、M35T、T319S、I434T、I460V突变的Mac-bFS，在野生型的洋甘菊来源的法尼烯合酶(Accession：AIG92847.1)的基础上引入了突变，其核苷酸序列如SEQ ID NO.1所示；
构建质粒pBFS1(含野生型的法尼烯合酶)的过程如下：(1)用引物pBFS1-1F/pBFS1-1R从Mac-bFS的核苷酸序列扩增获得片段。(2)随后Golden Gate assembly的方法克隆到pZY900获得质粒pBFS1。
构建质粒pBFS45(图20)的过程如下：(1)用引物pBFS1-1F/pBFS45-6R从F11S、M35T、T319S、I434T、I460V突变的Mac-bFS的核苷酸序列扩增获得片段。(2)随后通过重叠延伸PCR将这些片段连接起来，再通过Golden Gate assembly的方法克隆到pZY900获得质粒pBFS45，所述质粒pBFS45编码区(即经过密码子优化bFS基因)的核苷酸序列如SEQ ID NO.1所示。构建质粒pBFS45所用引物序列见下表12：
表12-质粒pBFS45构建所需引物
3、质粒pBFS45-P4
(1)用表13所示的引物pAFS1-P2-1F/R、pAFS1-P2-3F/pBFS45-P4-3R、pBFS45-P4-5F/pBFS45-P4-5R、pBFS45-P4-7F/pAFS1-P2-7R、pAFS1-P2-8F/R分别从酿酒酵母菌株CEN.PK2-1D扩增获得Ura3 left arm、tCYC1、pGAL10-pGAL1、tPGK1、Ura3 right arm。
(2)用表13所示的引物pAFS1-P2-2F/R从pRS423(ATCC87327)扩增获得His3marker；
(3)用表13所示的引物pBFS45-P4-4F/R、pBFS45-P4-6F/R从上述步骤构建获得的质粒pBFS45中扩增获得Mac-bFS(F11S，M35T，T319S，I434T，I460V)；
(4)用引物pAFS1-P2-9F/R从pRS426扩增获得质粒骨架；
(5)随后将所述步骤获得的这些片段组装，获得质粒pBFS45-P4。
4、质粒pBFS45-P7
(1)用表13所示的引物pAFS1-P5-1F/R、pAFS1-P5-3F/pBFS45-P7-3R、pBFS45-P7-5F/R、pBFS45-P7-7F/pAFS1-P5-7R、pAFS1-P5-8F/R分别从酿酒酵母菌株CEN.PK2-1D扩增获得YPRCdelta15 left arm、tCYC1、pGAL10-pGAL1、tPGK1、YPRCdelta15 right arm。
(2)用表13所示的引物pAFS1-P5-2F/R从pRS424扩增获得Trp1 marker；
(3)用表13所示的引物pBFS45-P7-4F/R、pBFS45-P7-6F/R从pBFS45扩增获得Mac-bFS(F11S，M35T，T319S，I434T，I460V)；
(4)用表13所示的引物pAFS1-P5-9F/R从pRS426扩增获得质粒骨架，随后这些片段组装获得质粒pBFS45-P7。构建上述质粒所用引物序列见下表13：
表13-质粒pBFS45-P4和pBFS45-P7构建所需引物
实施例4、β-法尼烯高产菌株的构建
1、用Mssi线性化实施例3所构建的质粒pBFS45、pBFS1，回收带有目的基因的片段，转化至酿酒酵母JCR27，根据同源重组来整合到染色体上，其整合位点为LEU2，筛选标记为亮氨酸，构建菌株JVA122、JVA94。经过摇瓶发酵，菌株JVA94产量为203mg/L，菌株JVA122产量为437mg/L。表明法尼烯合酶的突变对于产量的提升有重要作用。
2、线性化实施例3所构建的质粒pBFS45-P4，回收带有目的基因的片段，转化至酿酒酵母JVA122，根据同源重组来整合到染色体上，其整合位点为URA3，筛选标记为组氨酸，构建菌株JVA129。
3、线性化实施例3所构建的pBFS45-P7，回收带有目的基因的片段，转化至酿酒酵母JVA129，根据同源重组来整合到染色体上，其整合位点为YPRCdelta15，筛选标记为色氨酸，构建菌株JVA139。
4、将所述菌株JVA122、JVA129和JVA139分别经过摇瓶发酵，这些菌株都能展现出良好得性能，β-法尼烯产量能达到较高水平，产量范围为790mg/L。
实施例5、生产β-法尼烯同时高产泛酸合成载体的构建
本发明实施例涉及到的相关基因包括：酵母本底的基因乙酰乳酸合酶(Acetolactate synthase，ilv2)；乙酰乳酸合酶调节亚基(Acetolactate synthase smallsubunit，ilv6)；异构酶(Ketol-acid reductoisomerase，ilv5)；二羟酸脱水酶(Dihydroxy-acid dehydratase，ilv3)；羟甲基转移酶(3-methyl-2-oxobutanoatehydroxymethyl transferase，Ecm31p)；酮泛解酸还原酶(Ketopantoate reductase,pan5)；D-泛酸合酶(pantothenate synthase，pan6)；大肠杆菌来源的经过酿酒酵母密码子优化后合成的基因天冬氨酸-1-脱羧酶(Aspartate 1-decarboxylase，PAND)。
1、质粒pXZ162(如图4所示)的构建：
用引物1621-F/R从pKlURA3 100(具体来源见下方内容)扩增获得pSNR52_gRNA.rDNA,KlURA3。再通过Golden Gate assembly的方法克隆到pCAS(具体来源见下方内容)获得质粒pXZ162。其中，所述pKlURA3 100、pCAS来源：A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomyces cerevisiae；
表14-质粒pXZ162构建所需引物
| 引物-pXZ162 | 序列(5’-3’) |
| 1621-F | aaaggtctcagatctccaaagtgacaggtgccccgttttagagctagaaatagcaagtt(SEQ ID NO.125) |
| 1621-R | aaaggtctcaaaactctagactttttcgatgatgtagtttc(SEQ ID NO.126) |
2、质粒pPT1(如图1所示)的构建：
(1)用引物1471-F/1471-R从酵母S288C基因组扩增获得rDNA left arm，(2)用引物1472-F/pPT1-2R从酵母基因组30000B扩增获得tCYC1。(3)用引物pPT1-3F/3R从30000B扩增获得Ilv2。(4)用引物pPT1-4F/4R从30000B扩增获得pGAL1-pGAL10。(5)用引物pPT1-5F/5R从30000B扩增获得Ilv5。(6)用引物pPT1-6F/6R从30000B扩增获得tGAL10-pGAL7。(7)用引物pPT1-7F/7R从30000B扩增获得Ilv3。(8)用引物pPT1-8F/pPT2-8R从30000B扩增获得tADH1。(9)用引物pPT2-9F/pPT1-9R从30000B扩增获得pGAL2。(10)用引物pPT1-10F/pPT1-10R从30000B扩增获得Ilv6。(11)用引物pPT1-11F/pPT2-11R从30000B扩增获得tERG20。(12)用引物pPT2-12F/1474-R从酵母S288C基因组扩增获得rDNA Right arm。(13)通过重叠延伸PCR将所述片段连接成一个片段，随后和pMD19T(可购买自TAKARA公司#D102A)通过gibson组装获得质粒pPT1。
表15-质粒pPT1构建所需引物
3、质粒pPT2(如图2所示)的构建：
(1)用引物1471-F/1471-R从酵母S288C基因组扩增获得rDNA left arm，(2)用引物1472-F/pPT3-2R从30000B扩增获得tCYC1。(3)用引物pPT3-3F/3R从30000B扩增获得Ecm31P。(4)用引物pPT3-4F/4R从30000B扩增获得pGAL1-pGAL10。(5)用引物pPT3-5F/5R从30000B扩增获得Pan5。(6)用引物pPT3-6F/6R从30000B扩增获得tGAL10-pGAL7。(7)用引物pPT3-7F/7R从30000B扩增获得Pan6。(8)用引物pPT3-8F/pPT2-8R从30000B扩增获得tADH1。(9)用引物pPT2-9F/pPT2-9R从30000B扩增获得pGAL2。(10)用引物pPT2-10F/pPT2-10R从PanD-Sc扩增获得PanD。(11)用引物pPT2-11F/11R从30000B扩增获得tERG20。(12)用引物pPT2-12F/1474-R从S288C扩增获得rDNA Right arm。(13)通过重叠延伸PCR将片段连接成一个片段，随后和pMD19T通过gibson组装获得质粒pPT2。
表16-质粒pPT2构建所需引物
4、质粒pPT3(如图3所示)的构建：
(1)用引物1471-F/1471-R从S288C扩增获得rDNA left arm，(2)用引物1472-F/pPT3-2R从30000B扩增获得tCYC1。(3)用引物pPT3-3F/3R从30000B扩增获得Ecm31P。(4)用引物pPT3-4F/4R从30000B扩增获得pGAL1-pGAL10。(5)用引物pPT3-5F/5R从30000B扩增获得Pan5。(6)用引物pPT3-6F/6R从30000B扩增获得tGAL10-pGAL7。(7)用引物pPT3-7F/7R从30000B扩增获得Pan6。(8)用引物pPT3-8F/8R从30000B扩增获得tADH1。(9)用引物pPT3-9F/1474-R从S288C扩增获得rDNA Right arm。(10)通过重叠延伸PCR将片段连接成一个片段，随后和pMD19T通过gibson组装获得质粒pPT3。
表17-质粒pPT3构建所需引物
实施例6、同时生产β-法尼烯和高产泛酸菌株的构建
1、用Mssi线性化实施例5构建所得质粒pPT1、pPT2，回收带有目的基因的片段，和实施例5构建所得的质粒pXZ162共转化至酿酒酵母JVA139，通过crispr-cas9基因编辑实现多位点整合到染色体上，其整合位点为rDNA，筛选标记为尿嘧啶，构建菌株JFS1。
2、将所述菌株JFS1，进行GAL80基因的敲除(敲除盒pZY521的构建：以酵母30000B的基因组为模板，使用引物5201-F/5211-R扩增获得GAL80的左同源臂；以pZY900为模板，使用引物5212-F/5212-R扩增获得筛选标记URA3；使用引物5213-F/5203-R扩增获得GAL80的右同源臂)以消除发酵过程中半乳糖的使用，构建获得菌株JFS2。
3、用Mssi线性化质粒pPT1、pPT3，回收带有目的基因的片段，和pXZ162共转化至酿酒酵母JVA139，通过crispr-cas9基因编辑实现多位点整合到染色体上，其整合位点为rDNA，筛选标记为尿嘧啶，构建菌株JFS3。
4、菌株JFS3，进行GAL80基因的敲除(同上)，构建获得菌株JFS4。
上述引物序列见下表18：
表18
| 引物 | 序列(5’-3’) |
| 5201-F | caatggtctaggtagtggcattcg(SEQ ID NO.193) |
| 5211-R | cgactcactatagggcgaattgggtacgacgggagtggaaagaacgg(SEQ ID NO.194) |
| 5212-F | tcccgttctttccactcccgtcgtacccaattcgccctatagtgag(SEQ ID NO.195) |
| 5212-R | gccaagcacagggcaagatgctttcacagcttgtctgtaagcgga(SEQ ID NO.196) |
| 5213-F | gcatccgcttacagacaagctgtgaaagcatcttgccctgtgctt(SEQ ID NO.197) |
| 5203-R | gagaccaccaagaatacagaagctattat(SEQ ID NO.198) |
实施例7、同时生产β-法尼烯和泛酸菌株的发酵罐发酵
参照文献(van Hoek,P.；de Hulster,E.；van Di jken,J.P.；Pronk,J.T.Fermentative capacity in high-cell-density fed-batch cultures of baker’syeast.Biotechnol.Bioeng.2000,68,517-523.)中所记载的发酵培养基，对实施例6所构建的菌株JFS2进行分批补料发酵，在发酵过程中添加覆盖剂以实现原位萃取，覆盖剂可为正十二烷、肉豆蔻酸异丙酯、棕榈酸异丙酯、液体石蜡、白油。发酵过程控制溶氧在20％以上，pH为5，葡萄糖浓度为1-2g/L，乙醇浓度为5g/L以下。最终在发酵罐上，菌株法尼烯产量为35g/L，泛酸产量为1.5g/L。
同样的方法对实施例6所构建的JFS4进行发酵，区别在于发酵中添加终浓度为15g/L的beta-丙氨酸。最终在发酵罐上，菌株法尼烯产量为45g/L，泛酸产量为6g/L。
实施例8、同时高产角鲨烯合成载体的构建
本发明实施例涉及到的相关基因包括：酵母本底的NADH激酶(POS5)、乙醇脱氢酶(ADH2)、乙酰辅酶A合成酶(ACS2)、乙醛脱氢酶(ALD6)，角鲨烯合酶(ERG9)
1、质粒pXZ159(如图6所示)的构建过程：
(1)以酿酒酵母菌株CEN.PK2-1D基因组为模板，用引物1591-F和1591-R通过PCR扩增得到片段1591(含EXG1位点左同源臂)，(2)用引物1593-F和1593-R通过PCR扩增得到片段1593(含POS5)；(3)用引物1595-F和1595-R通过PCR扩增得到片段1595(含EXG1位点右同源臂)；(4)以pRS426(ATCC87333)为模板，用引物1592-F和1592-R通过PCR扩增得到片段1592(含GAL1启动子)，(5)用引物1594-F和1594-R通过PCR扩增得到片段1594(含CYC1终止子)；(6)使用Gibson组装方法将片段与pMD19T载体进行组装，大肠杆菌转化，酶切验证和测序正确后得到得到质粒pXZ159。
2、质粒pXZ161(如图7所示)的构建过程：
pXZ161是靶向DPP1和EXG1位点的gRNA质粒，用于Crispr-Cas9基因编辑技术中靶点识别切割酿酒酵母基因组DNA的工具质粒。(1)以pstgRNA质粒(A gRNA-tRNA array forCRISPR-Cas9 based rapid multiplexed genome editing in Saccharomycescerevisiae)为模板，用引物1611-F和1611-R通过PCR扩增得到片段1611(含DPP1位点gRNA，gRNA scaffold，tGly和部分EXG1位点gRNA)；(2)以pKlURA3 100质粒(所述pKlURA3 100的来源：A gRNA-tRNA array for CRISPR-Cas9 based rapid multiplexed genome editingin Saccharomyces cerevisiae；)为模板，用引物1612-F和1612-R通过PCR扩增得到片段1612(含EXG1位点gRNA，gRNA scaffold，SUF17终止子和URA筛选标签)；(3)使用GoldenGate组装方法将片段与pCas9载体进行组装，大肠杆菌转化，酶切验证和测序正确后得到质粒pXZ161。
3、质粒pXZ171(如图5所示)的构建过程：
(1)以酿酒酵母菌株CEN.PK2-1D基因组为模板，用引物1711-F和1711-R通过PCR扩增得到片段1711(含DPP1位点左同源臂)，(2)用引物1712-F和1712-R通过PCR扩增得到片段1712(含GAL1启动子)，(3)用引物1713-F和1713-R通过PCR扩增得到片段1713(含ADH2和ADH2终止子)，(4)用引物1714-F和1714-R通过PCR扩增得到片段1714(含GAL10启动子)，(5)用引物1715-F和1715-R通过PCR扩增得到片段1715(含ACS2)，(6)用引物1716-F和1716-R通过PCR扩增得到片段1716(含GAL10终止子和GAL7启动子)，(7)用引物1717-F和1717-R通过PCR扩增得到片段1717(含ALD6)，(8)用引物1719-F和1719-R通过PCR扩增得到片段1719(含DPP1位点右同源臂)；(9)以pRS426(ATCC87333)为模板，用引物1718-F和1718-R通过PCR扩增得到片段1718(含CYC1终止子)；(10)以片段1712、片段1713和片段1714为模板，用引物1712-F和1714-R通过OE-PCR得到片段17110；(11)以片段1715和片段1716为模板，用引物1715-F和1716-R通过OE-PCR得到片段17111；(12)以片段1717和片段1718为模板，用引物1717-F和1718-R通过OE-PCR得到片段17112；(13)使用Gibson组装方法将片段1711，17110，17111，17112，1719与pMD19T载体进行组装，大肠杆菌转化，酶切验证和测序正确后得到得到质粒pXZ171。
4、质粒pSX01(如图8所示)的构建过程：
(1)以酿酒酵母菌株CEN.PK2-1D基因组为模板，用1471-F/pSX01-1R从S288C扩增获得rDNA left arm。(2)用pSX01-2F/R从酵母S288C基因组扩增获得pHXT1。(3)用pSX01-3F/R从S288C扩增获得ERG9。(4)用pSX01-4F/pPT2-11R从30000B扩增获得tERG20。(5)用pPT2-12F/1474-R从酵母S288C基因组扩增获得rDNA RIGHT arm。(6)将上述片段通过重叠延伸PCR连接，随后和pMD19T载体进行组装，大肠杆菌转化，酶切验证和测序正确后得到得到质粒pSX01。
表19-实施例8中载体构建所需引物
实施例9、同时生产β-法尼烯和高产角鲨烯菌株的构建
1、用Mssi线性化质粒pXZ171、pXZ159，回收带有目的基因的片段，和pXZ161共转化至酿酒酵母JVA139，通过crispr-cas9基因编辑实现多位点整合到染色体上，其整合位点为DPP1和exg1，筛选标记为尿嘧啶，构建菌株JSX1。
2、用Mssi线性化质粒pSX01，回收带有目的基因的片段，和pXZ162共转化至酿酒酵母JSX1，通过crispr-cas9基因编辑实现多位点整合到染色体上，其整合位点为rDNA，筛选标记为尿嘧啶，构建菌株JSX2。
3、将所述菌株JSX2，进行GAL80基因的敲除(同上)，构建获得菌株JSX3。
实施例10、同时生产β-法尼烯和高产角鲨烯菌株的发酵罐发酵
参照文献(van Hoek,P.；de Hulster,E.；van Di jken,J.P.；Pronk,J.T.Fermentative capacity in high-cell-density fed-batch cultures of baker’syeast.Biotechnol.Bioeng.2000,68,517-523.)中所记载的发酵培养基，对实施例9所构建的菌株JSX3进行分批补料发酵，在发酵过程中添加覆盖剂以实现原位萃取，覆盖剂可为正十二烷、肉豆蔻酸异丙酯、棕榈酸异丙酯、液体石蜡、白油。发酵过程控制溶氧在20％以上，pH为5，葡萄糖浓度为1-2g/L。最终在发酵罐上，菌株法尼烯产量为37g/L，角鲨烯产量为7.5g/L。
实施例11、同时生产β-法尼烯和高产脂肪酸所需质粒的构建
本发明实施例涉及到的相关基因包括：敲除脂肪酸降解相关基因酰基CoA合成酶FAA1，FAA4，β-氧化酶POX1，过表达本底乙酰辅酶A羧化酶ACC1、脂肪酸合成酶复合体FAS1和FAS2，大肠杆菌来源的截短信号肽的硫解酶tesA。
本发明实施例涉及到的原理：同时进行法尼烯和脂肪酸的生产，两者合成均会竞争底物乙酰辅酶A，使得法尼烯的产量明显下降。所以构建质粒，使用葡萄糖诱导型启动子pHXT1实现在发酵前期开启脂肪酸的合成，发酵后期实现法尼烯的合成。
1、质粒pZF01(如图9所示)构建过程：
(1)以S288C基因组为模板，用pZF01-1F/1R从S288C扩增获得FAA1 left arm。(2)用pZF01-2F/2R从S288C扩增获得pHXT1。(3)用pZF01-3F/3R从MG1655基因组扩增获得截短的tesA。(4)用pZF01-4F/4R从30000B扩增获得tERG20。(5)用pZF01-5F/5R从S288C扩增获得FAA1 RIGHT arm。(6)将上述片段通过重叠延伸PCR连接，随后和pMD19T载体进行组装，大肠杆菌转化，酶切验证和测序正确后得到得到质粒pZF01。
表20-质粒pZF01构建所需引物
2、质粒pZF02(如图10所示)构建过程：
(1)以S288C基因组为模板，用pZF02-1F/1R从S288C扩增获得FAA4 left arm。(2)用pZF02-2F/2R从S288C扩增获得pHXT1。(3)用pZF02-3F/3R从S288C扩增获得ACC1。(4)用pZF02-4F/4R从30000B扩增获得tERG20。(4)用pZF02-5F/5R从S288C扩增获得FAA4 RIGHTarm。(5)将上述片段通过重叠延伸PCR连接，随后和pMD19T载体进行组装，大肠杆菌转化，酶切验证和测序正确后得到得到质粒pZF02。
表21-质粒pZF02构建所需引物
| 引物 | 序列(5’-3’) |
| pZF02-1F | gatcctctagagattgtttaaacctcggccttcgttcatctc(SEQ ID NO.251) |
| pZF02-1R | tattccagatgagacctgcatttgatgcgtacttcttagttttt(SEQ ID NO.252) |
| pZF02-2F | agaagtacgcatcaaatgcaggtctcatctggaatat(SEQ ID NO.253) |
| pZF02-2R | gaataagctttcttcgctcatgattttacgtatatcaactagttg(SEQ ID NO.254) |
| pZF02-3F | gatatacgtaaaatcatgagcgaagaaagcttattcg(SEQ ID NO.255) |
| pZF02-3R | cgattagcgttagttttatttcaaagtcttcaacaatttttct(SEQ ID NO.256) |
| pZF02-4F | gaagactttgaaataaaactaacgctaatcgataaaacattag(SEQ ID NO.257) |
| pZF02-4R | gtaaaaaactatgtcttcctttaaaaaaaatccttggactagtcacg(SEQ ID NO.258) |
| pZF02-5F | aggattttttttaaaggaagacatagttttttactttccc(SEQ ID NO.259) |
| pZF02-5R | ctgcaggtcgacgatgtttaaacagtcataaattcaaatatcccgtt(SEQ ID NO.260) |
3、质粒pZF03(如图11所示)构建过程：
(1)基因合成XhoI-pHXT1-pHXT1-AvrII；(2)以S288C基因组为模板，用pZF03-1F/1R从S288C扩增获得POX1 left arm。(3)用pZF03-2F/2R从S288C扩增获得tACT1。(4)用pZF03-3F/3R从S288C扩增获得FAS2。(5)用pZF03-4F/4R从S288C扩增获得FAS1。(6)用pZF03-5F/5R从30000B扩增获得tPGK1。(7)用pZF03-6F/6R从S288C扩增获得POX1 rightarm。(8)用pZF03-7F/7R从pMD19T扩增获得载体骨架。(9)将上述片段通过重叠延伸PCR连接，随后和上述基因合成获得的XhoI-pHXT1-pHXT1-AvrII进行酶切酶连，大肠杆菌转化，酶切验证和测序正确后得到得到质粒pZF03。
表22-质粒pZF03构建所需引物
| 引物 | 序列(5’-3’) |
| pZF03-1F | gatcctctagagattgcggccgcgtcaaagcactaatacattgggg(SEQ ID NO.261) |
| pZF03-1R | tttatccattggaccgtgtaatcgcaatactaatttattatattttcttttt(SEQ ID NO.262) |
| pZF03-2F | taaattagtattgcgattacacggtccaatggataaa(SEQ ID NO.263) |
| pZF03-2R | ccgtttctactaagaaatagtctctgcttttgtgcgcgt(SEQ ID NO.264) |
| pZF03-3F | gcgcacaaaagcagagactatttcttagtagaaacggcgac(SEQ ID NO.265) |
| pZF03-3R | aaatcctcgagatgaagccggaagttgagc(SEQ ID NO.266) |
| pZF03-4F | cgtaaaatccctaggatggacgcttactccacaag(SEQ ID NO.267) |
| pZF03-4R | gatttcaattcaattcaatttaggattgttcatacttttcccag(SEQ ID NO.268) |
| pZF03-5F | aacaatcctaaattgaattgaattgaaatcgatagatca(SEQ ID NO.269) |
| pZF03-5R | gaaaacaggaaacctctacaacgaacgcagaattttcgag(SEQ ID NO.270) |
| pZF03-6F | gaaaattctgcgttcgttgtagaggtttcctgttttcct(SEQ ID NO.271) |
| pZF03-6R | gcctgcaggtcgacgatgcggccgctggtaaatgtagtcatgtcattgattc(SEQ ID NO.272) |
| pZF03-7F | catttaccagcggccgcatcgtcgacctgcaggca(SEQ ID NO.273) |
| pZF03-7R | gtgctttgacgcggccgcaatctctagaggatccccgg(SEQ ID NO.274) |
4、质粒pZF04(如图12所示)构建过程：
pZF04是靶向FAA1、FAA4和POX1位点的gRNA质粒，用于Crispr-Cas9基因编辑技术中靶点识别切割酿酒酵母基因组DNA的工具质粒。(1)以pstgRNA质粒(A gRNA-tRNA arrayfor CRISPR-Cas9 based rapid multiplexed genome editing in Saccharomycescerevisiae)为模板，用引物pZF04-1F和pZF04-1R通过PCR扩增得到含POX1位点gRNA，gRNAscaffold，tGly和部分FAA1位点gRNA)；(2)以pstgRNA质粒为模板，用引物pZF04-2F和pZF04-2R通过PCR扩增得到含部分FAA1位点gRNA，gRNA scaffold，tGly和部分FAA4位点gRNA)；(3)以pKlURA3 100质粒(所述pKlURA3 100的来源：A gRNA-tRNA array forCRISPR-Cas9based rapid multiplexed genome editing in Saccharomycescerevisiae；)为模板，用引物pZF04-3F和pZF04-3R通过PCR扩增得到片段含FAA4位点gRNA，gRNA scaffold，SUF17终止子和URA筛选标签)；(4)使用Golden Gate组装方法将片段与pCas9载体进行组装，大肠杆菌转化，酶切验证和测序正确后得到质粒pZF04。
表23-质粒pZF04构建过程所需引物
| 引物 | 序列(5’-3’) |
| pZF04-1F | aaaggtctcagatccacatttttagagtcatcccgttttagagctagaaatagcaagtt(SEQ ID NO.275) |
| pZF04-1R | aaaggtctcacctgtcctcagatgcgcaagcccggaatcgaaccggg(SEQ ID NO.276) |
| pZF04-2F | aaaggtctcacaggaggcaaaggttttagagctagaaatagcaagtta(SEQ ID NO.277) |
| pZF04-2R | aaaggtctcatcttcaggattctgcgcaagcccggaatcgaaccggg(SEQ ID NO.278) |
| pZF04-3F | aaaggtctcaaagaaactgctggttttagagctagaaatagcaagtta(SEQ ID NO.279) |
| pZF04-3R | aaaggtctcaaaactctagactttttcgatgatgtagtttc(SEQ ID NO.280) |
实施例12、同时生产β-法尼烯和高产脂肪酸菌株的构建
1、用Mssi线性化质粒pZF01、pZF02，NotI线性化质粒pZF03，回收带有目的基因的片段，和pZF04共转化至酿酒酵母JVA139，通过crispr-cas9基因编辑实现多位点整合到染色体上，其整合位点为POX1、FAA1、FAA4，筛选标记为尿嘧啶，构建菌株JZF01。
2、将所述菌株JZF01，进行GAL80基因的敲除(敲除盒pZY521)以消除发酵过程中半乳糖的使用，构建获得菌株JZF02。
实施例13、法尼烯和脂肪酸分时空合成菌株的发酵罐发酵
参照文献(van Hoek,P.；de Hulster,E.；van Di jken,J.P.；Pronk,J.T.Fermentative capacity in high-cell-density fed-batch cultures of baker’syeast.Biotechnol.Bioeng.2000,68,517-523.)中所记载的发酵培养基，对实施例12所构建的菌株JZF02进行分批补料发酵，在发酵过程中添加覆盖剂以实现原位萃取，覆盖剂可为正十二烷、肉豆蔻酸异丙酯、棕榈酸异丙酯、液体石蜡、白油。发酵过程控制pH为5。调控葡萄糖浓度使得在发酵前期积累脂肪酸，发酵后期积累法尼烯。最终菌株法尼烯产量为43g/L，自由脂肪酸产量为6.8g/L。
实施例14、同时生产β-法尼烯和维生素B2所需质粒的构建
1、质粒pVB1(如图13所示)的构建过程：
(1)用引物1471-F/1471-R从S288C扩增获得rDNA left arm，(2)用引物1472-F/pVB1-2R从30000B扩增获得tCYC1。用引物pVB1-3F/3R从30000B扩增获得RIB1。(3)用引物pVB1-4F/4R从30000B扩增获得pGAL1-pGAL10。(4)用引物pVB1-5F/5R从30000B扩增获得RIB7。(5)用引物pVB1-6F/6R从30000B扩增获得tGAL10-pGAL7。(6)用引物pVB1-7F/7R从30000B扩增获得RIB2。(7)用引物pVB1-8F/8R从30000B扩增获得tADH1。(8)用引物pVB1-9F/1474-R从S288C扩增获得rDNA Right arm。(9)通过重叠延伸PCR将片段连接成一个片段，随后和pMD19T通过gibson组装获得质粒pVB1。
表24-质粒pVB1构建过程所需引物
2、质粒pVB2(如图14所示)构建过程：
(1)用引物1471-F/1471-R从S288C扩增获得rDNA left arm，(2)用引物1472-F/pVB2-2R从30000B扩增获得tCYC1。(3)用引物pVB2-3F/3R从30000B扩增获得RIB3。(4)用引物pVB2-4F/4R从30000B扩增获得pGAL1-pGAL10。(5)用引物pVB2-5F/5R从30000B扩增获得RIB4。(6)用引物pVB2-6F/6R从30000B扩增获得tGAL10-pGAL7。(7)用引物pVB2-7F/7R从30000B扩增获得RIB5。(8)用引物pVB2-8F/8R从30000B扩增获得tADH1。(9)用引物pVB2-9F/1474-R从S288C扩增获得rDNA Right arm。(10)通过重叠延伸PCR将片段连接成一个片段，随后和pMD19T通过gibson组装获得质粒pVB2。
表25-质粒pVB2构建过程所需引物
实施例15、同时生产β-法尼烯和维生素B2所需菌株的构建
1、用Mssi线性化质粒pVB1、pVB2,回收带有目的基因的片段，和pXZ162共转化至酿酒酵母JVA139，通过crispr-cas9基因编辑实现多位点整合到染色体上，其整合位点为rDNA，筛选标记为尿嘧啶，构建菌株JVB01。
2、将所述菌株JVB01，进行GAL80基因的敲除(敲除盒pZY521)以消除发酵过程中半乳糖的使用，构建获得菌株JVB02。
实施例16、法尼烯和维生素B2合成菌株的发酵罐发酵
参照文献(van Hoek,P.；de Hulster,E.；van Di jken,J.P.；Pronk,J.T.Fermentative capacity in high-cell-density fed-batch cultures of baker’syeast.Biotechnol.Bioeng.2000,68,517-523.)中所记载的发酵培养基，对实施例15所构建的菌株JVB02进行分批补料发酵，在发酵过程中添加覆盖剂以实现原位萃取，覆盖剂可为正十二烷、肉豆蔻酸异丙酯、棕榈酸异丙酯、液体石蜡、白油。发酵过程控制pH为5。菌株在生产法尼烯的同时可以高产维生素B2。最终菌株法尼烯产量为37g/L，维生素B2产量为2.5g/L。
上述实施例为本发明较佳的实施方式，但本发明的实施方式并不受上述实施例的限制，其他的任何未背离本发明的精神实质与原理下所作的改变、修饰、替代、组合、简化，均应为等效的置换方式，都包含在本发明的保护范围之内。
最后，还需要说明的是，术语“包括”、“包含”或者其任何其他变体意在涵盖非排他性的包含，从而使得包括一系列要素的过程、方法、物品或者设备不仅包括那些要素，而且还包括没有明确列出的其他要素，或者是还包括为这种过程、方法、物品或者设备所固有的要素。
尽管已描述了本发明的优选实施例，但本领域内的技术人员一旦得知了基本创造性概念，则可对这些实施例作出另外的变更和修改。所以，所附权利要求意欲解释为包括优选实施例以及落入本发明范围的所有变更和修改。
显然，本领域的技术人员可以对本发明进行各种改动和变型而不脱离本发明的精神和范围。这样，倘若本发明的这些修改和变型属于本发明权利要求及其等同技术的范围之内，则本发明也意图包含这些改动和变型在内。
序列表
<110> 武汉大学
<120> 一种同时生产倍半萜和初级代谢产物的重组微生物及其应用
<160> 317
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1725
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 1
atgtctacta tccctgtttc cagtgtttct tcctctagtt ctgcctctcc attggtattg 60
gatgataaat tatctaccaa gcaagatgtt gtcagacaca ccaccaactt ttctgcttct 120
atctggggtg accaattcct aacataccac gaaccagaag atttggtcat caagaagcaa 180
caagttgaac aattgaagga agaagttaag aaggaattga tggctatcaa gggttctaac 240
gacccacaac aacacatcaa attaatggaa ttgatcgact ctgttcaaag attgggtatc 300
gcttaccatt tcgaagaaga aattgaagaa gctttgcaac acattcacgt tacttatggt 360
gaacactggg ttgacaagga aaacttgcaa tccgtcagct tgtggttcag attactaaga 420
caacaaggtt tcaacgtttc ttccggtgtt ttcaaggact acatggacga aaaaggtaac 480
ttcaaggaat ctttgtgtaa cgatgctcaa ggtatcttgg ctttgtacga agctgcttac 540
atgagagttg aaggtgaaac cagattggac aaggctttgg aattcaccaa ggttcatttg 600
gatatcattt ctaaggatcc atcttgtgat tcttacttga gaactcgtgt tcaccaagcc 660
ctgaaacaac cattaagaag aagattggcc agaatcgaag cattacacta catgccagtc 720
taccaacaag actcttctca caacgaagtt ttactaaagt tggctaaggt cgatttctct 780
gttttgcaat ctatgcacaa gaaagaattg tcacatatct gtaagtggtg gaaggacttg 840
gatttgcaaa acaagttgcc atacgttcgt gacagagtcg ttgaaggtta cttctggatt 900
ttgtccatct actacgaacc tcaacatgcc agaaccagaa tgttcttgat gaagtcttgt 960
atgtggcttg ttgttttgga tgacactttt gacaattacg gtacctacga agagttggaa 1020
attttcgctc aagctgtcga aagatggagt atttcatgca tggatatgtt gccagaatat 1080
atgaaattga tttaccaaga attagttaac ttgcacgtcg acatggaaga atccttggaa 1140
aaggaaggta agacttacca aatccattac gtcaaggaaa tggccaagga attggtccgt 1200
aactacttgg tcgaagcccg ttggttgaag gagggttaca tgccaacctt agaagaatac 1260
atgtccgtat ccatggttac cggtacttac ggtttgatga ctgctagatc ctacgttggt 1320
agagacgaca ttgtcactga agacactttc aaatgggttt cttcttaccc accaatcgtc 1380
aaggcttcct gtgttattgt ccgtctgatg gacgatattg tctcccacaa ggaagaacaa 1440
gaaagaggtc acgttgcttc ttccattgaa tgttacagca aggaatctgg tgctaccgaa 1500
gaagaagctt gtgaatacat ctcctccaag gtcgaagacg cttggaaggt catcaacaga 1560
gaatcattaa gaccaactgc tgttccattc ccattgttga tgccagctat taatttggcc 1620
agaatgtgtg aagtcttata ctctgtcaac gatggtttca ctcacgctga aggcgacatg 1680
aagtcctata tgaagtccta ctttgtccac ccaatggtca tttga 1725
<210> 2
<211> 381
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 2
atgattagaa ccatgttgca aggaaagtta catagggtaa aggttactca cgccgatttg 60
cattatgagg gttcctgtgc tattgatcag gatttcctag acgctgcagg cattttagaa 120
aacgaagcga ttgacatctg gaatgttacc aatggtaaga gatttagtac atatgccatc 180
gctgcagaaa gaggcagcag aattatctca gtgaatggag ctgccgcgca ttgtgcttct 240
gttggcgata ttgtaattat agcatcattc gttactatgc cagacgaaga agctagaaca 300
tggagaccga atgtcgctta ctttgaaggt gataatgaaa tgaagagaac tgctaaagct 360
ataccagtcc aggttgcttg a 381
<210> 3
<211> 12416
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 3
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420
cctctagaga ttgtttaaac tttcctctaa tcaggttcca ccaaacagat accccggtgt 480
ttcacggaat ggtacgtttg atatcgctga tttgagagga ggttacactt gaagaatcac 540
agtcttgcga ccggctattc aacaaggcat tcccccaagt ttgaattctt tgaaatagat 600
tgctattagc tagtaatcca ccaaatcctt cgctgctcac caatggaatc gcaagatgcc 660
cacgatgaga ctgttcaggt taaacgcaaa agaaacacac tctgggaatt tcttcccaaa 720
ttgtatctct caatacgcat caacccatgt caattaaaca cgctgtatag agactaggca 780
gatctgacga tcacctagcg actctctcca ccgtttgacg aggccattta caaaaacata 840
acgaacgaca agcctactcg aattcgtttc caaactcttt tcgaacttgt cttcaactgc 900
tttcgcatga agtacctccc aactactttt cctcacactt gtactccatg actaaacccc 960
ccctcccatt acaaactaaa atcttacttt tattttcttt tgccctctct gtcgctctgc 1020
cttaactacg tatttctcgc cgagaaaaac ttcaatttaa gctattctcc aaaaatctta 1080
gcgtatattt tttttccaaa gtgagcaaat taaagccttc gagcgtccca aaaccttctc 1140
aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ttgtacagaa aaaaaagaaa 1200
aatttgaaat ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac 1260
ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg ggggagggcg 1320
tgaatgtaag cgtgacataa ctaattacat gatatcgaca aaggaaaagg ggcctgttca 1380
gtgcttaccg cctgtacgct tatgacgtaa ttcagtctgt tgtctttcaa cttctgggtc 1440
aaaatttatg aactcgtcta gaccgctacc acctgccacc attggcaaaa caggaacttt 1500
tttatcaact tccacttcaa gcaaaactgg gcccttggta gaaacgaatt ctttcaactt 1560
agcgtccaat tcctcttgct tcttgactct taaacctttt aaacccatag cctccgctag 1620
ttttatgaaa tcagggttca attgatgtgt gtgggaataa cgatgttcgt agaacaggga 1680
ttgccattga gtaaccatac cttgctcttc attgttcaaa atcaaaatct tcactggagt 1740
accagcttga acggcagaac tcaattccgt tagagtcatg ttaaaggatg cgtcaccatc 1800
aatgtcaata accaaagatt ctggctttgc aacttgagca ccgatggcgg cagggagacc 1860
gtaacccatc gtacctaaac cacctgatgt gatgaaagta tgtggatttc tccatgtcca 1920
gtgttgagca gcccacattt gatgttgccc cacacccgtt gtaacaatga catgtcttcc 1980
tgtgtcgttg gcaaccttgg atagtttctt tataaccgtc tgtggtttaa ttttagatcc 2040
tggagtctcc tccatataag cgtatgggta ttccttcttc catttattta tttgagcaaa 2100
ccattcagac ctctccttaa ctgggaaaat ctttgacatc attttgccca gattggtcgt 2160
agcatcacct tccactgcta tttgagtttg aacaaccttg tttatgtttt ttggactaac 2220
ctcgaaatga ataataccac ctctaccctc ggcagctgca cgacgagctt ctggagcgaa 2280
tttagaaata ttaccagtga cacggtcgtc gaatctagca ccaactgcaa ttatcaagtc 2340
ggcattttgc actgccaggt tggcagtagc acaaccgtgc ataccaagca tatccaatga 2400
ttttggatct tcttggtcga atgaacctaa accttgtaaa gtagtggtga caggtatttg 2460
agcacggtca cttaattctt ttagtaatct tggaccatct gcatggttta aaataccagc 2520
accgacgtat aagacaggtt tctttgccaa gttgatcaaa tctgctgctt tattgatact 2580
ttgcatgaca aattcatctt gtgcgcgact ggttaattgg tttagtgcgt ttgatggaag 2640
agttgttttt gttggaattg gatttcttaa gatagctgct gtaacatcct ttggtaaatc 2700
gaccaagact ggtcccggtc taccgctcgt ggcaatttca aaagcctcgt taatacgcaa 2760
tggcaattct tccacggact tgaccatgac attccatttc gtacaagatc tagaaatacc 2820
aacgacgtca gcctcttgga aagcatcagt accgatagca ctagttggga cttgccctgt 2880
aaagacaacc attggaatcc cgtctgcaaa ggcatctgcc attggagtaa cgacattggt 2940
ggcacctggc ccagaagtaa ccaagacaac acctggttta ccagaagctc tggcgtagcc 3000
ttctgccatg tgaccggcac cttgttcgtg ttttggaaga acgaagttga atttatcact 3060
gttatgaatg gcatcgtaaa caggtaggat agcaccacct ggataaccaa atacagtatc 3120
aacgttttgt ctggacatca tttcgttaaa tatttgacca ccagttaaac cgacgaaaga 3180
ggtatccatg tcaggctcag cgcgtagttt cttagccaat tttgaaggtt cagcgggctg 3240
ttctaatgga tcaacattga aacttggagc aggctctggc cttttagagg ctggtaatgg 3300
agacgcactg taataacggg aagacgaact ataaaagcgc tgcgcgagag ctactgatct 3360
catggcaggt gtgttgcggt atgctatatg ttgaaagcaa cgcttaatag cgaagttttt 3420
tagcgtagat tgtctgatca ttatagtttt ttctccttga cgttaaagta tagaggtata 3480
ttaacaattt tttgttgata cttttatgac atttgaataa gaagtaatac aaaccgaaaa 3540
tgttgaaagt attagttaaa gtggttatgc agcttttgca tttatatatc tgttaataga 3600
tcaaaaatca tcgcttcgct gattaattac cccagaaata aggctaaaaa actaatcgca 3660
ttattatcct atggttgtta atttgattcg ttgatttgaa ggtttgtggg gccaggttac 3720
tgccaatttt tcctcttcat aaccataaaa gctagtattg tagaatcttt attgttcgga 3780
gcagtgcggc gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga ccaggacgca 3840
cggaggagag tcttccgtcg gagggctgtc gcccgctcgg cggcttctaa tccgtacttc 3900
aatatagcaa tgagcagtta agcgtattac tgaaagttcc aaagagaagg tttttttagg 3960
ctaagataat ggggctcttt acatttccac aacatataag taagattaga tatggatatg 4020
tatatggtgg tattgccatg taatatgatt attaaacttc tttgcgtcca tccaaaaaaa 4080
aagtaagaat ttttgaaaat tcaatataaa tgttgagaac tcaagccgcc agattgatct 4140
gcaactcccg tgtcatcact gctaagagaa cctttgcttt ggccacccgt gctgctgctt 4200
acagcagacc agctgcccgt ttcgttaagc caatgatcac tacccgtggt ttgaagcaaa 4260
tcaacttcgg tggtactgtt gaaaccgtct acgaaagagc tgactggcca agagaaaagt 4320
tgttggacta cttcaagaac gacacttttg ctttgatcgg ttacggttcc caaggttacg 4380
gtcaaggttt gaacttgaga gacaacggtt tgaacgttat cattggtgtc cgtaaagatg 4440
gtgcttcttg gaaggctgcc atcgaagacg gttgggttcc aggcaagaac ttgttcactg 4500
ttgaagatgc tatcaagaga ggtagttacg ttatgaactt gttgtccgat gccgctcaat 4560
cagaaacctg gcctgctatc aagccattgt tgaccaaggg taagactttg tacttctccc 4620
acggtttctc cccagtcttc aaggacttga ctcacgttga accaccaaag gacttagatg 4680
ttatcttggt tgctccaaag ggttccggta gaactgtcag atctttgttc aaggaaggtc 4740
gtggtattaa ctcttcttac gccgtctgga acgatgtcac cggtaaggct cacgaaaagg 4800
cccaagcttt ggccgttgcc attggttccg gttacgttta ccaaaccact ttcgaaagag 4860
aagtcaactc tgacttgtac ggtgaaagag gttgtttaat gggtggtatc cacggtatgt 4920
tcttggctca atacgacgtc ttgagagaaa acggtcactc cccatctgaa gctttcaacg 4980
aaaccgtcga agaagctacc caatctctat acccattgat cggtaagtac ggtatggatt 5040
acatgtacga tgcttgttcc accaccgcca gaagaggtgc tttggactgg tacccaatct 5100
tcaagaatgc tttgaagcct gttttccaag acttgtacga atctaccaag aacggtaccg 5160
aaaccaagag atctttggaa ttcaactctc aacctgacta cagagaaaag ctagaaaagg 5220
aattagacac catcagaaac atggaaatct ggaaggttgg taaggaagtc agaaagttga 5280
gaccagaaaa ccaataattt gccagcttac tatccttctt gaaaatatgc actctatatc 5340
ttttagttct taattgcaac acatagattt gctgtataac gaattttatg ctatttttta 5400
aatttggagt tcagtgataa aagtgtcaca gcgaatttcc tcacatgtag ggaccgaatt 5460
gtttacaagt tctctgtacc accatggaga catcaaagat tgaaaatcta tggaaagata 5520
tggacggtag caacaagaat atagcacgag ccgcgaagtt catttcgtta cttttgatat 5580
cgctcacaac tattgcgaag cgcttcagtg aaaaaatcat aaggaaaagt tgtaaatatt 5640
attggtagta ttcgtttggt aaagtagagg gggtaatttt tcccctttat tttgttcata 5700
cattcttaaa ttgctttgcc tctccttttg gaaagctata cttcggagca ctgttgagcg 5760
aaggctcatt agatatattt tctgtcattt tccttaaccc aaaaataagg gaaagggtcc 5820
aaaaagcgct cggacaactg ttgaccgtga tccgaaggac tggctataca gtgttcacaa 5880
aatagccaag ctgaaaataa tgtgtagcta tgttcagtta gtttggctag caaagatata 5940
aaagcaggtc ggaaatattt atgggcatta ttatgcagag catcaacatg ataaaaaaaa 6000
acagttgaat attccctcaa aaatgggctt gttaacgaaa gttgctacat ctagacaatt 6060
ctctacaacg agatgcgttg caaagaagct caacaagtac tcgtatatca tcactgaacc 6120
taagggccaa ggtgcgtccc aggccatgct ttatgccacc ggtttcaaga aggaagattt 6180
caagaagcct caagtcgggg ttggttcctg ttggtggtcc ggtaacccat gtaacatgca 6240
tctattggac ttgaataaca gatgttctca atccattgaa aaagcgggtt tgaaagctat 6300
gcagttcaac accatcggtg tttcagacgg tatctctatg ggtactaaag gtatgagata 6360
ctcgttacaa agtagagaaa tcattgcaga ctcctttgaa accatcatga tggcacaaca 6420
ctacgatgct aacatcgcca tcccatcatg tgacaaaaac atgcccggtg tcatgatggc 6480
catgggtaga cataacagac cttccatcat ggtatatggt ggtactatct tgcccggtca 6540
tccaacatgt ggttcttcga agatctctaa aaacatcgat atcgtctctg cgttccaatc 6600
ctacggtgaa tatatttcca agcaattcac tgaagaagaa agagaagatg ttgtggaaca 6660
tgcatgccca ggtcctggtt cttgtggtgg tatgtatact gccaacacaa tggcttctgc 6720
cgctgaagtg ctaggtttga ccattccaaa ctcctcttcc ttcccagccg tttccaagga 6780
gaagttagct gagtgtgaca acattggtga atacatcaag aagacaatgg aattgggtat 6840
tttacctcgt gatatcctca caaaagaggc ttttgaaaac gccattactt atgtcgttgc 6900
aaccggtggg tccactaatg ctgttttgca tttggtggct gttgctcact ctgcgggtgt 6960
caagttgtca ccagatgatt tccaaagaat cagtgatact acaccattga tcggtgactt 7020
caaaccttct ggtaaatacg tcatggccga tttgattaac gttggtggta cccaatctgt 7080
gattaagtat ctatatgaaa acaacatgtt gcacggtaac acaatgactg ttaccggtga 7140
cactttggca gaacgtgcaa agaaagcacc aagcctacct gaaggacaag agattattaa 7200
gccactctcc cacccaatca aggccaacgg tcacttgcaa attctgtacg gttcattggc 7260
accaggtgga gctgtgggta aaattaccgg taaggaaggt acttacttca agggtagagc 7320
acgtgtgttc gaagaggaag gtgcctttat tgaagccttg gaaagaggtg aaatcaagaa 7380
gggtgaaaaa accgttgttg ttatcagata tgaaggtcca agaggtgcac caggtatgcc 7440
tgaaatgcta aagccttcct ctgctctgat gggttacggt ttgggtaaag atgttgcatt 7500
gttgactgat ggtagattct ctggtggttc tcacgggttc ttaatcggcc acattgttcc 7560
cgaagccgct gaaggtggtc ctatcgggtt ggtcagagac ggcgatgaga ttatcattga 7620
tgctgataat aacaagattg acctattagt ctctgataag gaaatggctc aacgtaaaca 7680
aagttgggtt gcacctccac ctcgttacac aagaggtact ctatccaagt atgctaagtt 7740
ggtttccaac gcttccaacg gttgtgtttt agatgcttga gcgaatttct tatgatttat 7800
gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc 7860
ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct 7920
ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccgggg ttgcaatttc 7980
tttttctatt agtagctaaa aatgggtcac gtgatctata ttcgaaaggg gcggttgcct 8040
caggaaggca ccggcggtct ttcgtccgtg cggagatatc tgcgccgttc aggggtccat 8100
gtgccttgga cgatattaag gcagaaggca gtatcggggc ggatcactcc gaaccgagat 8160
tagttaagcc cttcccatct caagatgggg agcaaatggc attatactcc tgctagaaag 8220
ttaactgtgc acatattctt aaattataca acattctgga gagctattgt tcaaaaaaca 8280
aacatttcgc aggctaaaat gtggagatag gataagtttt gtagacatat ataaacaatc 8340
agtaattgga ttgaaaattt ggtgttgtga attgctcttc attatgcacc ttattcaatt 8400
atcatcaaga atagtaatag ttaagtaaac acaagattaa cataataaaa aaaataattc 8460
tttcataatg ctgagatcgt tattgcaaag cggccaccgc agggtggttg cttcttcatg 8520
tgctaccatg gtgcgttgca gttcctcgtc gacctccgcg ttggcgtaca agcagatgca 8580
cagacacgca acaagacctc ccttgcccac actagacact ccttcctgga atgccaacag 8640
tgccgtttca tccatcattt acgaaacacc agcgccttct cgtcaaccaa gaaaacagca 8700
tgtcttgaac tgtttggtgc aaaacgaacc cggtgtcttg tccagagtct cgggtacgtt 8760
agctgccaga ggctttaaca tcgattcgtt ggtcgtgtgc aacaccgagg tcaaagacct 8820
aagtagaatg accattgttt tgcaagggca agatggcgta gtcgaacaag cacgcagaca 8880
aatcgaagac ttggtccccg tctacgccgt cctagactat accaattctg agatcatcaa 8940
aagagagcta gtgatggcca gaatctctct attgggtact gaatacttcg aagacctact 9000
attgcaccac cacacttcca ccaatgctgg cgccgctgac tcccaagaat tggtcgccga 9060
aatcagagaa aagcaattcc accctgccaa cttgcccgcc agtgaggtat taaggttgaa 9120
gcacgagcat ttgaacgata tcaccaactt gaccaacaac tttggaggtc gtgtcgtcga 9180
catcagcgaa acaagctgta ttgtggagtt gtctgcaaaa cccacacgta tctctgcctt 9240
cttgaagttg gtcgagccat tcggtgtcct agagtgtgca agaagcggta tgatggcatt 9300
gccaagaact cctttgaaga caagcaccga ggaagctgcc gacgaagacg aaaagatcag 9360
cgaaatcgtc gacatttccc aactaccacc tggttagaac taacgctaat cgataaaaca 9420
ttagatttca aactagataa ggaccatgta taagaactat atacttccaa tataatatag 9480
tataagcttt aagatagtat ctctcgatct accgttccac gtgactagtc caaggatttt 9540
ttttaacagg tgccccgggt aacccagttc ctcactattt tttactgcgg aagcggaagc 9600
ggaaaatacg gaaacgcgcg ggaacataca aaacatacaa aatatacctt tctcacacaa 9660
gaaatatatg ctacttgcaa aatatcatac caaaaaactt ttcacaaccg aaaccaaaac 9720
caacggatat catacattac actaccacca ttcaaacttt actactatcc tcccttcagt 9780
ttcccttttt ctgccttttt cggtgacgga aatacgcttc agagacccta aagggaaatc 9840
catgccataa caggaaagta acatcccaat gcggactata ccaccccacc acactcctac 9900
caataacggt aactattcta tgttttctta ctcctatgtc tattcatctt tcatctgact 9960
acctaatact atgcaaaaat gtaaaatcat cacacaaaac ataaacaatc aaaatcagcc 10020
atttccgcac cttttcctct gtccactttc aaccgtccct ccaaatgtaa aatggcctat 10080
cggaatacat tttctacatc ctaactacta taaaacaacc tttagactta cgtttgctac 10140
tctcatggtt taaacatcgt cgacctgcag gcatgcaagc ttggcgtaat catggtcata 10200
gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 10260
cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 10320
ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 10380
acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 10440
gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 10500
gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 10560
ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 10620
cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 10680
ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 10740
taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 10800
ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 10860
ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 10920
aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 10980
tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac 11040
agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 11100
ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 11160
tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 11220
tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 11280
cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 11340
aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 11400
atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 11460
cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 11520
tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 11580
atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 11640
taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 11700
tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 11760
gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 11820
cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 11880
cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 11940
gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 12000
aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 12060
accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 12120
ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 12180
gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 12240
aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 12300
taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 12360
cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc 12416
<210> 4
<211> 9866
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 4
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420
cctctagaga ttgtttaaac tttcctctaa tcaggttcca ccaaacagat accccggtgt 480
ttcacggaat ggtacgtttg atatcgctga tttgagagga ggttacactt gaagaatcac 540
agtcttgcga ccggctattc aacaaggcat tcccccaagt ttgaattctt tgaaatagat 600
tgctattagc tagtaatcca ccaaatcctt cgctgctcac caatggaatc gcaagatgcc 660
cacgatgaga ctgttcaggt taaacgcaaa agaaacacac tctgggaatt tcttcccaaa 720
ttgtatctct caatacgcat caacccatgt caattaaaca cgctgtatag agactaggca 780
gatctgacga tcacctagcg actctctcca ccgtttgacg aggccattta caaaaacata 840
acgaacgaca agcctactcg aattcgtttc caaactcttt tcgaacttgt cttcaactgc 900
tttcgcatga agtacctccc aactactttt cctcacactt gtactccatg actaaacccc 960
ccctcccatt acaaactaaa atcttacttt tattttcttt tgccctctct gtcgctctgc 1020
cttaactacg tatttctcgc cgagaaaaac ttcaatttaa gctattctcc aaaaatctta 1080
gcgtatattt tttttccaaa gtgagcaaat taaagccttc gagcgtccca aaaccttctc 1140
aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ttgtacagaa aaaaaagaaa 1200
aatttgaaat ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac 1260
ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg ggggagggcg 1320
tgaatgtaag cgtgacataa ctaattacat gatatcgaca aaggaaaagg ggcctgttta 1380
cttttcgtta attgaagaga ggaattcatt ccataagtcc tccttaactt tgaaagtatg 1440
agtacctctc tcaggaaatg tcctatcctc tacacttgca atgtactcct tcaagccctg 1500
agtggcaata tctgtcatat ttacagcttg cttcacaaat tttgggacag aatcgccttg 1560
catcccgaga aggtcagata taactagaac ttgtccactg gtaccgttac ctgcaccgat 1620
acctattgtt ggtactgaga gtttagatgt tatgaactga gccatcttat gggggacaca 1680
ttcaatcagg atagaccaac agccaatttt ttgtagttgc atggcggttt catacagctc 1740
ctgcatttgt aatagacttt tgctaccttg cactttataa cctccaagtg aatgaacttt 1800
ttggggagtt agtcctatat gagccataac aggtataccc cgggagcaca gttcttcaat 1860
aaatttcatt gcatatttgt ctttggtgta agaaccgact tcaactttga ctgaggtgac 1920
tttactgtcc aatttcatga tatctatggc attcttcaag ccatcagata tgcccgattc 1980
aaacgtaccg aatggcatgt ctaccactac catggaagaa ccttcagctc tgcacacaga 2040
ggcaacatga tatttaaact cattcaagga tagtgtaatt gtgctatcgt accccaatga 2100
agtcatcgcc aaagaatctc cgactaagag taaatcacaa ttagccttgt taacccatgt 2160
tgcagtaatg aagtcatacg cagtacacat cgacaaaggc gtaccagtaa aatatttgtt 2220
cctgatatcc tgaatcgtat tatatttgac aacgtttttt gctgtagaaa aaaatcgctt 2280
agaggaggtg cataattgtc ttttcattat attcattata gttttttctc cttgacgtta 2340
aagtatagag gtatattaac aattttttgt tgatactttt atgacatttg aataagaagt 2400
aatacaaacc gaaaatgttg aaagtattag ttaaagtggt tatgcagctt ttgcatttat 2460
atatctgtta atagatcaaa aatcatcgct tcgctgatta attaccccag aaataaggct 2520
aaaaaactaa tcgcattatt atcctatggt tgttaatttg attcgttgat ttgaaggttt 2580
gtggggccag gttactgcca atttttcctc ttcataacca taaaagctag tattgtagaa 2640
tctttattgt tcggagcagt gcggcgcgag gcacatctgc gtttcaggaa cgcgaccggt 2700
gaagaccagg acgcacggag gagagtcttc cgtcggaggg ctgtcgcccg ctcggcggct 2760
tctaatccgt acttcaatat agcaatgagc agttaagcgt attactgaaa gttccaaaga 2820
gaaggttttt ttaggctaag ataatggggc tctttacatt tccacaacat ataagtaaga 2880
ttagatatgg atatgtatat ggtggtattg ccatgtaata tgattattaa acttctttgc 2940
gtccatccaa aaaaaaagta agaatttttg aaaattcaat ataaatgact gcaccacaca 3000
gaagtactat tcatattctt gggttgggtg ccatgggtac cgttttggca gttgaccttt 3060
taagatttac caatgcccta gttgtgccct tatttagatc acaagaaaga cttgcacaat 3120
ttcagaagac aaatgggaac aacatttcga ttcgcaaact ttatttggaa ggctcgccaa 3180
tattttctta tccagtggag aaatgcgaat gccctgaaac tttctctaaa aaacctattg 3240
acaacttagt cgtcactaca aagacttacc aaaccaaaga agcactagct ccttatttgc 3300
cttatattaa taaaaataca aacttaattt tgattcagaa tggactgggc gttttagaac 3360
tattgagaga agaaatcttt acagattcaa aaaacagacc acatttattt cagggtgtca 3420
tttctcatgg cgtttatcag gataaagcag gagttttcaa tcatgctggg tgggctggta 3480
tgaaaattgc caaattgcct tggactgagg aagaaatgat tcaaaaaaaa tccgtagtcg 3540
aggacgatgc agccaataac tcattagtaa aattattaac agaaccaaaa tttgccaagg 3600
aatttggaat agaacattcc acttatcaag aaatgctctt tggtcaatta tttaaatttt 3660
tggttaatgc atgcatgaat ccagttacgg ctattctaga ttgtgtcaat ggtgagatga 3720
aggccagttg cggtccagtt ttcacatcta ttattgacga gtgtttacaa atattgagag 3780
ttgcttatag gccattgttt caataccacg aaaaatacag cggtaatgaa gaatacccag 3840
aaatggatgt caatgcagta ctgacaaccg ataacatggt ctccgaagtc actagaattg 3900
gctgtgacat caattctagg aacagtagtt caatgagaca agacactttg ttcttaagag 3960
acattgaaat tgagtatatc aatggctatg tcgtcaaatt ggctgataac ttaaatctag 4020
acccaaactg ctgcaaggtt aataaaacta tcggagaatt ggccactatg agattagcat 4080
tgaacaggtc gagaagtatc aatggtgact ggagaaagga ctgatttgcc agcttactat 4140
ccttcttgaa aatatgcact ctatatcttt tagttcttaa ttgcaacaca tagatttgct 4200
gtataacgaa ttttatgcta ttttttaaat ttggagttca gtgataaaag tgtcacagcg 4260
aatttcctca catgtaggga ccgaattgtt tacaagttct ctgtaccacc atggagacat 4320
caaagattga aaatctatgg aaagatatgg acggtagcaa caagaatata gcacgagccg 4380
cgaagttcat ttcgttactt ttgatatcgc tcacaactat tgcgaagcgc ttcagtgaaa 4440
aaatcataag gaaaagttgt aaatattatt ggtagtattc gtttggtaaa gtagaggggg 4500
taatttttcc cctttatttt gttcatacat tcttaaattg ctttgcctct ccttttggaa 4560
agctatactt cggagcactg ttgagcgaag gctcattaga tatattttct gtcattttcc 4620
ttaacccaaa aataagggaa agggtccaaa aagcgctcgg acaactgttg accgtgatcc 4680
gaaggactgg ctatacagtg ttcacaaaat agccaagctg aaaataatgt gtagctatgt 4740
tcagttagtt tggctagcaa agatataaaa gcaggtcgga aatatttatg ggcattatta 4800
tgcagagcat caacatgata aaaaaaaaca gttgaatatt ccctcaaaaa tgaaaatctt 4860
ccatactgtc gaagaagttg ttcaatggag aacacaggag ctgagggaaa ctagatttag 4920
agaaactatt gggttcgttc ccacaatggg ttgcctgcat tcgggtcacg ctagtttgat 4980
ctcgcagtct gtgaaggaaa acacctatac tgtggtcagt atatttgtaa atccctccca 5040
gtttgcgcca acggaagatc tagataacta tcctcgaact ttgccagacg acatcaaatt 5100
gcttgagtcg ttgaaggtgg atgttctatt tgctcctaat gcacacgtga tgtatccaca 5160
gggaattccg ctcgacatag aagagcagaa aggccctttt gttagtgttc ttggattgag 5220
tgaaaaatta gaggggaaga cgagacctaa cttctttagg ggcgtggcaa ctgtcgtgac 5280
taaactattc aatatcgtta tggcggatgt ggcttatttt gggcagaagg acattcaaca 5340
gttcattgtt ttacagtgta tggtggacga actgtttgtt aatacaaggc tacaaatgat 5400
gcctattgta agaaacaata atggactggc tctgagtagt agaaacaaat atctttgtcc 5460
agagtcttta aagatctctg aaaaccttta ccgcgggctg aaagctgcgg aaaatgctat 5520
taggagacta gcaccagggg gacgtctctc cagatcagaa atcatcgata ctgtgactca 5580
aatatgggca ccctacgttg attcccacga tttcaaaatc gactatgttt ccttagcaga 5640
ttttaagact cttgatgaac tctccgatgt tgaaaacacc agcgaacagc agccaatagt 5700
cattagttgt gctgtatacg tgactgaccg cgaaaaaccc gatacggtcg tcagactaat 5760
agataacatc gttatttaag cgaatttctt atgatttatg atttttatta ttaaataagt 5820
tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt 5880
cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt 5940
cgctcttatt gaccacacct ctaccggggt tgcaatttct ttttctatta gtagctaaaa 6000
atgggtcacg tgatctatat tcgaaagggg cggttgcctc aggaaggcac cggcggtctt 6060
tcgtccgtgc ggagatatct gcgccgttca ggggtccatg tgccttggac gatattaagg 6120
cagaaggcag tatcggggcg gatcactccg aaccgagatt agttaagccc ttcccatctc 6180
aagatgggga gcaaatggca ttatactcct gctagaaagt taactgtgca catattctta 6240
aattatacaa cattctggag agctattgtt caaaaaacaa acatttcgca ggctaaaatg 6300
tggagatagg ataagttttg tagacatata taaacaatca gtaattggat tgaaaatttg 6360
gtgttgtgaa ttgctcttca ttatgcacct tattcaatta tcatcaagaa tagtaatagt 6420
taagtaaaca caagattaac ataataaaaa aaataattct ttcataatga ttagaaccat 6480
gttgcaagga aagttacata gggtaaaggt tactcacgcc gatttgcatt atgagggttc 6540
ctgtgctatt gatcaggatt tcctagacgc tgcaggcatt ttagaaaacg aagcgattga 6600
catctggaat gttaccaatg gtaagagatt tagtacatat gccatcgctg cagaaagagg 6660
cagcagaatt atctcagtga atggagctgc cgcgcattgt gcttctgttg gcgatattgt 6720
aattatagca tcattcgtta ctatgccaga cgaagaagct agaacatgga gaccgaatgt 6780
cgcttacttt gaaggtgata atgaaatgaa gagaactgct aaagctatac cagtccaggt 6840
tgcttgaaac taacgctaat cgataaaaca ttagatttca aactagataa ggaccatgta 6900
taagaactat atacttccaa tataatatag tataagcttt aagatagtat ctctcgatct 6960
accgttccac gtgactagtc caaggatttt ttttaacagg tgccccgggt aacccagttc 7020
ctcactattt tttactgcgg aagcggaagc ggaaaatacg gaaacgcgcg ggaacataca 7080
aaacatacaa aatatacctt tctcacacaa gaaatatatg ctacttgcaa aatatcatac 7140
caaaaaactt ttcacaaccg aaaccaaaac caacggatat catacattac actaccacca 7200
ttcaaacttt actactatcc tcccttcagt ttcccttttt ctgccttttt cggtgacgga 7260
aatacgcttc agagacccta aagggaaatc catgccataa caggaaagta acatcccaat 7320
gcggactata ccaccccacc acactcctac caataacggt aactattcta tgttttctta 7380
ctcctatgtc tattcatctt tcatctgact acctaatact atgcaaaaat gtaaaatcat 7440
cacacaaaac ataaacaatc aaaatcagcc atttccgcac cttttcctct gtccactttc 7500
aaccgtccct ccaaatgtaa aatggcctat cggaatacat tttctacatc ctaactacta 7560
taaaacaacc tttagactta cgtttgctac tctcatggtt taaacatcgt cgacctgcag 7620
gcatgcaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 7680
cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg 7740
agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 7800
gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 7860
gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 7920
ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 7980
aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 8040
ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 8100
gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 8160
cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 8220
gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 8280
tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 8340
cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 8400
cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 8460
gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc 8520
agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 8580
cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 8640
tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 8700
tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 8760
ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 8820
cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 8880
cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 8940
accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 9000
ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 9060
ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 9120
tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 9180
acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 9240
tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 9300
actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 9360
ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 9420
aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 9480
ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 9540
cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 9600
aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 9660
actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 9720
cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 9780
ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 9840
taggcgtatc acgaggccct ttcgtc 9866
<210> 5
<211> 8837
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 5
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420
cctctagaga ttgtttaaac tttcctctaa tcaggttcca ccaaacagat accccggtgt 480
ttcacggaat ggtacgtttg atatcgctga tttgagagga ggttacactt gaagaatcac 540
agtcttgcga ccggctattc aacaaggcat tcccccaagt ttgaattctt tgaaatagat 600
tgctattagc tagtaatcca ccaaatcctt cgctgctcac caatggaatc gcaagatgcc 660
cacgatgaga ctgttcaggt taaacgcaaa agaaacacac tctgggaatt tcttcccaaa 720
ttgtatctct caatacgcat caacccatgt caattaaaca cgctgtatag agactaggca 780
gatctgacga tcacctagcg actctctcca ccgtttgacg aggccattta caaaaacata 840
acgaacgaca agcctactcg aattcgtttc caaactcttt tcgaacttgt cttcaactgc 900
tttcgcatga agtacctccc aactactttt cctcacactt gtactccatg actaaacccc 960
ccctcccatt acaaactaaa atcttacttt tattttcttt tgccctctct gtcgctctgc 1020
cttaactacg tatttctcgc cgagaaaaac ttcaatttaa gctattctcc aaaaatctta 1080
gcgtatattt tttttccaaa gtgagcaaat taaagccttc gagcgtccca aaaccttctc 1140
aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ttgtacagaa aaaaaagaaa 1200
aatttgaaat ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac 1260
ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg ggggagggcg 1320
tgaatgtaag cgtgacataa ctaattacat gatatcgaca aaggaaaagg ggcctgttta 1380
cttttcgtta attgaagaga ggaattcatt ccataagtcc tccttaactt tgaaagtatg 1440
agtacctctc tcaggaaatg tcctatcctc tacacttgca atgtactcct tcaagccctg 1500
agtggcaata tctgtcatat ttacagcttg cttcacaaat tttgggacag aatcgccttg 1560
catcccgaga aggtcagata taactagaac ttgtccactg gtaccgttac ctgcaccgat 1620
acctattgtt ggtactgaga gtttagatgt tatgaactga gccatcttat gggggacaca 1680
ttcaatcagg atagaccaac agccaatttt ttgtagttgc atggcggttt catacagctc 1740
ctgcatttgt aatagacttt tgctaccttg cactttataa cctccaagtg aatgaacttt 1800
ttggggagtt agtcctatat gagccataac aggtataccc cgggagcaca gttcttcaat 1860
aaatttcatt gcatatttgt ctttggtgta agaaccgact tcaactttga ctgaggtgac 1920
tttactgtcc aatttcatga tatctatggc attcttcaag ccatcagata tgcccgattc 1980
aaacgtaccg aatggcatgt ctaccactac catggaagaa ccttcagctc tgcacacaga 2040
ggcaacatga tatttaaact cattcaagga tagtgtaatt gtgctatcgt accccaatga 2100
agtcatcgcc aaagaatctc cgactaagag taaatcacaa ttagccttgt taacccatgt 2160
tgcagtaatg aagtcatacg cagtacacat cgacaaaggc gtaccagtaa aatatttgtt 2220
cctgatatcc tgaatcgtat tatatttgac aacgtttttt gctgtagaaa aaaatcgctt 2280
agaggaggtg cataattgtc ttttcattat attcattata gttttttctc cttgacgtta 2340
aagtatagag gtatattaac aattttttgt tgatactttt atgacatttg aataagaagt 2400
aatacaaacc gaaaatgttg aaagtattag ttaaagtggt tatgcagctt ttgcatttat 2460
atatctgtta atagatcaaa aatcatcgct tcgctgatta attaccccag aaataaggct 2520
aaaaaactaa tcgcattatt atcctatggt tgttaatttg attcgttgat ttgaaggttt 2580
gtggggccag gttactgcca atttttcctc ttcataacca taaaagctag tattgtagaa 2640
tctttattgt tcggagcagt gcggcgcgag gcacatctgc gtttcaggaa cgcgaccggt 2700
gaagaccagg acgcacggag gagagtcttc cgtcggaggg ctgtcgcccg ctcggcggct 2760
tctaatccgt acttcaatat agcaatgagc agttaagcgt attactgaaa gttccaaaga 2820
gaaggttttt ttaggctaag ataatggggc tctttacatt tccacaacat ataagtaaga 2880
ttagatatgg atatgtatat ggtggtattg ccatgtaata tgattattaa acttctttgc 2940
gtccatccaa aaaaaaagta agaatttttg aaaattcaat ataaatgact gcaccacaca 3000
gaagtactat tcatattctt gggttgggtg ccatgggtac cgttttggca gttgaccttt 3060
taagatttac caatgcccta gttgtgccct tatttagatc acaagaaaga cttgcacaat 3120
ttcagaagac aaatgggaac aacatttcga ttcgcaaact ttatttggaa ggctcgccaa 3180
tattttctta tccagtggag aaatgcgaat gccctgaaac tttctctaaa aaacctattg 3240
acaacttagt cgtcactaca aagacttacc aaaccaaaga agcactagct ccttatttgc 3300
cttatattaa taaaaataca aacttaattt tgattcagaa tggactgggc gttttagaac 3360
tattgagaga agaaatcttt acagattcaa aaaacagacc acatttattt cagggtgtca 3420
tttctcatgg cgtttatcag gataaagcag gagttttcaa tcatgctggg tgggctggta 3480
tgaaaattgc caaattgcct tggactgagg aagaaatgat tcaaaaaaaa tccgtagtcg 3540
aggacgatgc agccaataac tcattagtaa aattattaac agaaccaaaa tttgccaagg 3600
aatttggaat agaacattcc acttatcaag aaatgctctt tggtcaatta tttaaatttt 3660
tggttaatgc atgcatgaat ccagttacgg ctattctaga ttgtgtcaat ggtgagatga 3720
aggccagttg cggtccagtt ttcacatcta ttattgacga gtgtttacaa atattgagag 3780
ttgcttatag gccattgttt caataccacg aaaaatacag cggtaatgaa gaatacccag 3840
aaatggatgt caatgcagta ctgacaaccg ataacatggt ctccgaagtc actagaattg 3900
gctgtgacat caattctagg aacagtagtt caatgagaca agacactttg ttcttaagag 3960
acattgaaat tgagtatatc aatggctatg tcgtcaaatt ggctgataac ttaaatctag 4020
acccaaactg ctgcaaggtt aataaaacta tcggagaatt ggccactatg agattagcat 4080
tgaacaggtc gagaagtatc aatggtgact ggagaaagga ctgatttgcc agcttactat 4140
ccttcttgaa aatatgcact ctatatcttt tagttcttaa ttgcaacaca tagatttgct 4200
gtataacgaa ttttatgcta ttttttaaat ttggagttca gtgataaaag tgtcacagcg 4260
aatttcctca catgtaggga ccgaattgtt tacaagttct ctgtaccacc atggagacat 4320
caaagattga aaatctatgg aaagatatgg acggtagcaa caagaatata gcacgagccg 4380
cgaagttcat ttcgttactt ttgatatcgc tcacaactat tgcgaagcgc ttcagtgaaa 4440
aaatcataag gaaaagttgt aaatattatt ggtagtattc gtttggtaaa gtagaggggg 4500
taatttttcc cctttatttt gttcatacat tcttaaattg ctttgcctct ccttttggaa 4560
agctatactt cggagcactg ttgagcgaag gctcattaga tatattttct gtcattttcc 4620
ttaacccaaa aataagggaa agggtccaaa aagcgctcgg acaactgttg accgtgatcc 4680
gaaggactgg ctatacagtg ttcacaaaat agccaagctg aaaataatgt gtagctatgt 4740
tcagttagtt tggctagcaa agatataaaa gcaggtcgga aatatttatg ggcattatta 4800
tgcagagcat caacatgata aaaaaaaaca gttgaatatt ccctcaaaaa tgaaaatctt 4860
ccatactgtc gaagaagttg ttcaatggag aacacaggag ctgagggaaa ctagatttag 4920
agaaactatt gggttcgttc ccacaatggg ttgcctgcat tcgggtcacg ctagtttgat 4980
ctcgcagtct gtgaaggaaa acacctatac tgtggtcagt atatttgtaa atccctccca 5040
gtttgcgcca acggaagatc tagataacta tcctcgaact ttgccagacg acatcaaatt 5100
gcttgagtcg ttgaaggtgg atgttctatt tgctcctaat gcacacgtga tgtatccaca 5160
gggaattccg ctcgacatag aagagcagaa aggccctttt gttagtgttc ttggattgag 5220
tgaaaaatta gaggggaaga cgagacctaa cttctttagg ggcgtggcaa ctgtcgtgac 5280
taaactattc aatatcgtta tggcggatgt ggcttatttt gggcagaagg acattcaaca 5340
gttcattgtt ttacagtgta tggtggacga actgtttgtt aatacaaggc tacaaatgat 5400
gcctattgta agaaacaata atggactggc tctgagtagt agaaacaaat atctttgtcc 5460
agagtcttta aagatctctg aaaaccttta ccgcgggctg aaagctgcgg aaaatgctat 5520
taggagacta gcaccagggg gacgtctctc cagatcagaa atcatcgata ctgtgactca 5580
aatatgggca ccctacgttg attcccacga tttcaaaatc gactatgttt ccttagcaga 5640
ttttaagact cttgatgaac tctccgatgt tgaaaacacc agcgaacagc agccaatagt 5700
cattagttgt gctgtatacg tgactgaccg cgaaaaaccc gatacggtcg tcagactaat 5760
agataacatc gttatttaag cgaatttctt atgatttatg atttttatta ttaaataagt 5820
tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt 5880
cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt 5940
cgctcttatt gaccacacct ctaccggcag gtgccccggg taacccagtt cctcactatt 6000
ttttactgcg gaagcggaag cggaaaatac ggaaacgcgc gggaacatac aaaacataca 6060
aaatatacct ttctcacaca agaaatatat gctacttgca aaatatcata ccaaaaaact 6120
tttcacaacc gaaaccaaaa ccaacggata tcatacatta cactaccacc attcaaactt 6180
tactactatc ctcccttcag tttccctttt tctgcctttt tcggtgacgg aaatacgctt 6240
cagagaccct aaagggaaat ccatgccata acaggaaagt aacatcccaa tgcggactat 6300
accaccccac cacactccta ccaataacgg taactattct atgttttctt actcctatgt 6360
ctattcatct ttcatctgac tacctaatac tatgcaaaaa tgtaaaatca tcacacaaaa 6420
cataaacaat caaaatcagc catttccgca ccttttcctc tgtccacttt caaccgtccc 6480
tccaaatgta aaatggccta tcggaataca ttttctacat cctaactact ataaaacaac 6540
ctttagactt acgtttgcta ctctcatggt ttaaacatcg tcgacctgca ggcatgcaag 6600
cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 6660
acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 6720
actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 6780
gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 6840
cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 6900
tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 6960
gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 7020
ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 7080
aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 7140
tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 7200
ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 7260
gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 7320
tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 7380
caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 7440
ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 7500
cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 7560
ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 7620
cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 7680
gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 7740
aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 7800
acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 7860
gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 7920
cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 7980
cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 8040
tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 8100
cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 8160
gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 8220
cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 8280
ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 8340
gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 8400
taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 8460
gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 8520
acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 8580
aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 8640
cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 8700
atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 8760
gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 8820
cacgaggccc tttcgtc 8837
<210> 6
<211> 9575
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 6
tgatccaata tcaaaggaaa tgatagcatt gaaggatgag actaatccaa ttgaggagtg 60
gcagcatata gaacagctaa agggtagtgc tgaaggaagc atacgatacc ccgcatggaa 120
tgggataata tcacaggagg tactagacta cctttcatcc tacataaata gacgcatata 180
agtacgcatt taagcataaa cacgcactat gccgttcttc tcatgtatat atatatacag 240
gcaacacgca gatataggtg cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc 300
attttcggaa gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa gttcctattc 360
tctagaaagt ataggaactt cagagcgctt ttgaaaacca aaagcgctct gaagacgcac 420
tttcaaaaaa ccaaaaacgc accggactgt aacgagctac taaaatattg cgaataccgc 480
ttccacaaac attgctcaaa agtatctctt tgctatatat ctctgtgcta tatccctata 540
taacctaccc atccaccttt cgctccttga acttgcatct aaactcgacc tctacatttt 600
ttatgtttat ctctagtatt actctttaga caaaaaaatt gtagtaagaa ctattcatag 660
agtgaatcga aaacaatacg aaaatgtaaa catttcctat acgtagtata tagagacaaa 720
atagaagaaa ccgttcataa ttttctgacc aatgaagaat catcaacgct atcactttct 780
gttcacaaag tatgcgcaat ccacatcggt atagaatata atcggggatg cctttatctt 840
gaaaaaatgc acccgcagct tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt 900
tttttatgga agagaaaata gacaccaaag tagccttctt ctaaccttaa cggacctaca 960
gtgcaaaaag ttatcaagag actgcattat agagcgcaca aaggagaaaa aaagtaatct 1020
aagatgcttt gttagaaaaa tagcgctctc gggatgcatt tttgtagaac aaaaaagaag 1080
tatagattct ttgttggtaa aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc 1140
agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa 1200
tgaagcacag attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa 1260
aatgcagctc agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttctac 1320
aaaatgaagc acagatgctt cgttcaggtg gcacttttcg gggaaatgtg cgcggaaccc 1380
ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 1440
gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 1500
cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 1560
tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 1620
tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 1680
cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 1740
tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 1800
agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 1860
ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 1920
ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 1980
aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 2040
gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 2100
tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 2160
ttgctgataa atctggagcc ggtgagcgtg ggagccgcgg tatcattgca gcactggggc 2220
cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 2280
atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 2340
cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 2400
ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 2460
cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 2520
ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 2580
tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 2640
taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 2700
caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 2760
agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 2820
gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 2880
gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 2940
ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 3000
acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 3060
tgtgatgctc gtcagggggg cggagcctat ggaaagcggc cgcacaatct ttgaaaagat 3120
aatgtatgat tatgctttca ctcatattta tacagaaact tgatgttttc tttcgagtat 3180
atacaaggtg attacatgta cgtttgaagt acaactctag attttgtagt gccctcttgg 3240
gctagcggta aaggtgcgca ttttttcaca ccctacaatg ttctgttcaa aagattttgg 3300
tcaaacgctg tagaagtgaa agttggtgcg catgtttcgg cgttcgaaac ttctccgcag 3360
tgaaagataa atgatctcca aagtgacagg tgccccgttt tagagctaga aatagcaagt 3420
taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gctttttttc 3480
ttttttgtcc accaccaaaa tgtcaatttt tttgccattt tctaaatagt tttcttcact 3540
ctacaagaga tatcacgatt gtttgctttc gatattggat ccatctaaag tcatttcaac 3600
tatctaagat gtggttgtga ttgggccatt ttgtgaaagc cagtacgcca gcgtcaatac 3660
actcccgtca attagttgca ccatgtccac aaaatcatat accagtagag ctgagactca 3720
tgcaagtccg gttgcatcga aacttttacg tttaatggat gaaaagaaga ccaatttgtg 3780
tgcttctctt gacgttcgtt cgactgatga gctattgaaa cttgttgaaa cgttgggtcc 3840
atacatttgc cttttgaaaa cacacgttga tatcttggat gatttcagtt atgagggtac 3900
tgtcgttcca ttgaaagcat tggcagagaa atacaagttc ttgatatttg aggacagaaa 3960
attcgccgat atcggtaaca cagtcaaatt acaatataca tcgggcgttt accgtatcgc 4020
agaatggtct gatatcacca acgcccacgg ggttactggt gctggtattg ttgctggctt 4080
gaaacaaggt gcgcaagagg tcaccaaaga accaagggga ttattgatgc ttgctgaatt 4140
gtcttccaag ggttctctag cacacggtga atatactaag ggtaccgttg atattgcaaa 4200
gagtgataaa gatttcgtta ttgggttcat tgctcagaac gatatgggag gaagagaaga 4260
agggtttgat tggctaatca tgaccccagg tgtaggttta gacgacaaag gcgatgcatt 4320
gggtcagcag tacagaaccg tcgacgaagt tgtaagtggt ggatcagata tcatcattgt 4380
tggcagagga cttttcgcca agggtagaga tcctaaggtt gaaggtgaaa gatacagaaa 4440
tgctggatgg gaagcgtacc aaaagagaat cagcgctccc cattaattat acaggaaact 4500
taatagaaca aatcacatat ttaatctaat agccacctgc attggcacgg tgcaacacta 4560
cttcaacttc atcttacaaa aagatcacgt gatctgttgt attgaactga aaattttttg 4620
tttgcttctc tctctctctc tttcattatg tgagatttaa aaaccagaaa ctacatcatc 4680
gaaaaagtct agagttttag agctagaaat agcaagttaa aataaggcta gtccgttatc 4740
aacttgaaaa agtggcaccg agtcggtgct ttttttattt tttgtcacta ttgcatgccg 4800
gtagaggtgt ggtcaataag agcgacctca tgctatacct gagaaagcaa cctgacctac 4860
aggaaagagt tactcaagaa taagaatttt cgttttaaaa cctaagagtc actttaaaat 4920
ttgtatacac ttattttttt tataacttat ttaataataa aaatcataaa tcataagaaa 4980
ttcgcctcga gtcagacctt tctcttcttt tttggagggt cacctcctag ctgactcaaa 5040
tcaatgcgtg tttcataaag accagtgatg gattgatgga taagagtggc atctaaaact 5100
tcttttgtag acgtatatcg tttacgatca attgttgtat caaaatattt aaaagcagcg 5160
ggagctccaa gattcgtcaa cgtaaataaa tgaataatat tttctgcttg ttcacgtatt 5220
ggtttgtctc tatgtttgtt atatgcacta agaactttat ctaaattggc atctgctaaa 5280
ataacacgct tagaaaattc actgatttgc tcaataatct catctaaata atgcttatgc 5340
tgctccacaa acaattgttt ttgttcgtta tcttctggac tacccttcaa cttttcataa 5400
tgactagcta aatataaaaa attcacatat ttgcttggca gagccagctc atttcctttt 5460
tgtaattctc cggcactagc cagcatccgt ttacgaccgt tttctaactc aaaaagacta 5520
tatttaggta gtttaatgat taagtctttt ttaacttcct tatatccttt agcttctaaa 5580
aagtcaatcg gatttttttc aaaggaactt ctttccataa ttgtgatccc tagtaactct 5640
ttaacggatt ttaacttctt cgatttccct ttttccacct tagcaaccac taggactgaa 5700
taagctaccg ttggactatc aaaaccacca tatttttttg gatcccagtc ttttttacga 5760
gcaataagct tgtccgaatt tctttttggt aaaattgact ccttggagaa tccgcctgtc 5820
tgtacttctg ttttcttgac aatattgact tggggcatgg acaatacttt gcgcactgtg 5880
gcaaaatctc gccctttatc ccagacaatt tctccagttt ccccattagt ttcgattaga 5940
gggcgtttgc gaatctctcc atttgcaagt gtaatttctg ttttgaagaa gttcatgata 6000
ttagagtaaa agaaatattt tgcggttgct ttgcctattt cttgctcaga cttagcaatc 6060
attttacgaa catcataaac tttataatca ccatagacaa actccgattc aagttttgga 6120
tatttcttaa tcaaagcagt tccaacgacg gcatttagat acgcatcatg ggcatgatgg 6180
taattgttaa tctcacgtac tttatagaat tggaaatctt ttcggaagtc agaaactaat 6240
ttagatttta aggtaatcac tttaacctct cgaataagtt tatcattttc atcgtattta 6300
gtattcatgc gactatccaa aatttgtgcc acatgcttag tgatttggcg agtttcaacc 6360
aattggcgtt tgataaaacc agctttatca agttcactca aacctccacg ttcagctttc 6420
gttaaattat caaacttacg ttgagtgatt aacttggcgt ttagaagttg tctccaatag 6480
tttttcatct ttttgactac ttcttcactt ggaacgttat ccgatttacc acgattttta 6540
tcagaacgcg ttaagacctt attgtctatt gaatcgtctt taaggaaact ttgtggaaca 6600
atgtgatcga catcataatc acttaaacga ttaatatcta attcttggtc cacatacatg 6660
tctcttccat tttggagata atagagatag agcttttcat tttgcaattg agtattttca 6720
acaggatgct ctttaagaat ctgacttcct aattctttga taccttcttc gattcgtttc 6780
atacgctctc gcgaattttt ctggcccttt tgagttgtct gattttcacg tgccatttca 6840
ataacgatat tttctggctt atgccgcccc attactttga ccaattcatc aacaactttt 6900
acagtctgta aaataccttt tttaatagca gggctaccag ctaaatttgc aatatgttca 6960
tgtaaactat cgccttgtcc agacacttgt gctttttgaa tgtcttcttt aaatgtcaaa 7020
ctatcatcat ggatcagctg cataaaattg cgattggcaa aaccatctga tttcaaaaaa 7080
tctaatattg ttttgccaga ttgcttatcc ctaataccat taatcaattt tcgagacaaa 7140
cgtccccaac cagtataacg gcgacgttta agctgtttca tcaccttatc atcaaagagg 7200
tgagcatatg ttttaagtct ttcctcaatc atctccctat cttcaaataa ggtcaatgtt 7260
aaaacaatat cctctaagat atcttcattt tcttcattat ccaaaaaatc tttatcttta 7320
ataattttta gcaaatcatg gtaggtacct aatgaagcat taaatctatc ttcaactcct 7380
gaaatttcaa cactatcaaa acattctatt tttttgaaat aatcttcttt taattgctta 7440
acggttactt ttcgatttgt tttgaagagt aaatcaacaa tggctttctt ctgttcacct 7500
gaaagaaatg ctggttttcg cattccttca gtaacatatt tgacctttgt caattcgtta 7560
taaaccgtaa aatactcata aagcaaacta tgttttggta gtactttttc atttggaaga 7620
tttttatcaa agtttgtcat gcgttcaata aatgattgag ctgaagcacc tttatcgaca 7680
acttcttcaa aattccatgg ggtaattgtt tcttcagact tccgagtcat ccatgcaaaa 7740
cgactattgc cacgcgccaa tggaccaaca taataaggaa ttcgaaaagt caagattttt 7800
tcaatcttct cacgattgtc ttttaaaaat ggataaaagt cttcttgtct tctcaaaata 7860
gcatgcagct cacccaagtg aatttgatgg gtaatagagc cgttgtcaaa ggtccgttgc 7920
ttgcgcagca aatcttcacg atttagtttc accaataatt cctcagtacc atccattttt 7980
tctaaaattg gtttgataaa tttataaaat tcttcttggc tagctccccc atcaatataa 8040
cctgcatatc cgttttttga ttgatcaaaa aagatttctt tatacttttc tggaagttgt 8100
tgtcgaacta aagcttttaa aagagtcaag tcttgatgat gttcatcgta gcgtttaatc 8160
attgaagctg ataggggagc cttagttatt tcagtattta ctcttaggat atctgaaagt 8220
aaaatagcat ctgataaatt cttagctgcc aaaaacaaat cagcatattg atctccaatt 8280
tgcgccaata aattatctaa atcatcatcg taagtatctt ttgaaagctg taatttagca 8340
tcttctgcca aatcaaaatt tgatttaaaa ttaggggtca aacccaatga caaagcaatg 8400
agattcccaa ataagccatt tttcttctca ccggggagct gagcaatgag attttctaat 8460
cgtcttgatt tactcaatcg tgcagaaaga atcgctttag catctactcc acttgcgtta 8520
atagggtttt cttcaaataa ttgattgtag gtttgtacca actggataaa tagtttgtcc 8580
acatcactat tatcaggatt taaatctccc tcaatcaaaa aatgaccacg aaacttaatc 8640
atatgcgcta aggccaaata gattaagcgc aaatccgctt tataagtaga atctaccaat 8700
ttttttcgca gatgatagat agttggatat ttctcatgat aagcaacttc atctactata 8760
tttccaaaaa taggatgacg ttcatgcttc ttgtcttctt ccaccaaaaa agactcttca 8820
agtcgatgaa agaaactatc atctactttc gccatctcat ttgaaaaaat ctcctgtaga 8880
taacaaatac gattcttccg acgtgtatac cttctacgag ctgtccgttt gagacgagtc 8940
gcttccgctg tctctccact gtcaaataaa agagccccta taagattttt tttgatactg 9000
tggcggtctg tatttcccag aaccttgaac tttttagacg gaaccttata ttcatcagtg 9060
atcaccgccc atccgacgct atttgtgccg atatctaagc ctattgagta tttcttatcg 9120
acctttctct tcttttttgg aggtttgtca tcgtcatctt tataatccat ctagaaaact 9180
tagattagat tgctatgctt tctttctaat gagcaagaag taaaaaaagt tgtaatagaa 9240
caagaaaaat gaaactgaaa cttgagaaat tgaagaccgt ttattaactt aaatatcaat 9300
gggaggtcat cgaaagagaa aaaaatcaaa aaaaaaattt tcaagaaaaa gaaacgtgat 9360
aaaaattttt attgcctttt tcgacgaaga aaaagaaacg aggcggcctc ttttttcttt 9420
tccaaacctt tagtacgggt aattaacgac accctagagg aagaaagagg ggaaatttag 9480
tatgctgtgc ttgggtgttt tgaagtggta cggcgatgcg cggagtccga gaaaatctgg 9540
aagagtaaaa aaggagtaga aacattttga agatc 9575
<210> 7
<211> 11509
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 7
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420
cctctagaga ttgtttaaac ctttttttta ttgtttcctg ttgtttttct ctttcttcct 480
tttctaattg ttctttagtt tctttgattc tttgtaatcg atcatcttcc aaggactgtg 540
tatttaccac attttgaaat tcgtccagtt tttcttgagt aaaagatgta atttcaaata 600
taatctcttg gcctcgggag gtgttgtgga ttttcttaaa ttcacttttc agcatttgca 660
attgactatc cattacattt tgtacatttt tgaattctat ttctggagcg gtataaggat 720
acattggggt catcgcaaaa tgtaatgtta tagaagattc aaccggctct ttgtcaacag 780
atcgaagcgt aatttcgaat ataatctgtg gctgcttatc ccagctagac tttcttttag 840
ttaagtcagt aaagtcatcc atataaatgg aacgtatcgc ttcaagttcg ttgcactgta 900
tttcataata ttgatctaaa gtgagatgac tcaatgacat ttatacatag tatgtgttaa 960
ggggaacgga aaattattga aaaaaaaaat caatgcttat aaaatcttaa aagacaacga 1020
ggctttccaa caatgtaaag attgaaaatt tagtgtattt aatgtccttt tgtaatgaaa 1080
ataacttctt gtaagaaaga agatagagat aaatgtaaaa gtaaatttgc ttaaaaagat 1140
tctaggctgt ttatagattg attacaccag gttcttatct caaaaatcag ctactcaact 1200
gcttcctaag aaactaccta catcactata gtccaactta aaacaaataa taaacttcta 1260
aggctttcgt gtaaagtgat gttgggattg tccgatgaaa taacaagcag gcccttgcac 1320
gtcaagatta atttgaataa agaagtaaca tgtcatcttt atgtaatcgc tgttattcca 1380
tacagaacaa taaatacgta tatttcgtat gtcatgtgga gtatatattc ttttttattc 1440
cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc 1500
ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa 1560
caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc 1620
tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat 1680
tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt 1740
aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg 1800
gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc 1860
tctatacttt aacgtcaagg agatgtctat tccagaaact caaaaagcca ttatcttcta 1920
cgaatccaac ggcaagttgg agcataagga tatcccagtt ccaaagccaa agcccaacga 1980
attgttaatc aacgtcaagt actctggtgt ctgccacacc gatttgcacg cttggcatgg 2040
tgactggcca ttgccaacta agttaccatt agttggtggt cacgaaggtg ccggtgtcgt 2100
tgtcggcatg ggtgaaaacg ttaagggctg gaagatcggt gactacgccg gtatcaaatg 2160
gttgaacggt tcttgtatgg cctgtgaata ctgtgaattg ggtaacgaat ccaactgtcc 2220
tcacgctgac ttgtctggtt acacccacga cggttctttc caagaatacg ctaccgctga 2280
cgctgttcaa gccgctcaca ttcctcaagg tactgacttg gctgaagtcg cgccaatctt 2340
gtgtgctggt atcaccgtat acaaggcttt gaagtctgcc aacttgagag caggccactg 2400
ggcggccatt tctggtgctg ctggtggtct aggttctttg gctgttcaat atgctaaggc 2460
gatgggttac agagtcttag gtattgatgg tggtccagga aaggaagaat tgtttacctc 2520
gctcggtggt gaagtattca tcgacttcac caaagagaag gacattgtta gcgcagtcgt 2580
taaggctacc aacggcggtg cccacggtat catcaatgtt tccgtttccg aagccgctat 2640
cgaagcttct accagatact gtagggcgaa cggtactgtt gtcttggttg gtttgccagc 2700
cggtgcaaag tgctcctctg atgtcttcaa ccacgttgtc aagtctatct ccattgtcgg 2760
ctcttacgtg gggaacagag ctgataccag agaagcctta gatttctttg ccagaggtct 2820
agtcaagtct ccaataaagg tagttggctt atccagttta ccagaaattt acgaaaagat 2880
ggagaagggc caaattgctg gtagatacgt tgttgacact tctaaataag cggatctctt 2940
atgtctttac gatttatagt tttcattatc aagtatgcct atattagtat atagcatctt 3000
tagatgacag tgttcgaagt ttcacgaata aaagataata ttctactttt tgctcccacc 3060
gcgtttgcta gcacgagtga acaccatccc tcgcctgtga gttgtaccca ttcctctaaa 3120
ctgtagacat ggtagcttca gcagtgttcg ttatgtacgg catcctccaa caaacagtcg 3180
gttatagttt gtcctgctcc tctgaatcgt ctccctcgat atttctcatg gccaggttac 3240
tgccaatttt tcctcttcat aaccataaaa gctagtattg tagaatcttt attgttcgga 3300
gcagtgcggc gcgaggcaca tctgcgtttc aggaacgcga ccggtgaaga cgaggacgca 3360
cggaggagag tcttccgtcg gagggctgtc gcccgctcgg cggcttctaa tccgtacttc 3420
aatatagcaa tgagcagtta agcgtattac tgaaagttcc aaagagaagg tttttttagg 3480
ctaagataat ggggctcttt acatttccac aacatataag taagattaga tatggatatg 3540
tatatggtgg taatgccatg taatatgatt attaaacttc tttgcgtcca tccaaaaaaa 3600
aagtaagaat ttttgaaaat tcaatataaa tgacaatcaa ggaacataaa gtagtttatg 3660
aagctcacaa cgtaaaggct cttaaggctc ctcaacattt ttacaacagc caacccggca 3720
agggttacgt tactgatatg caacattatc aagaaatgta tcaacaatct atcaatgagc 3780
cagaaaaatt ctttgataag atggctaagg aatacttgca ttgggatgct ccatacacca 3840
aagttcaatc tggttcattg aacaatggtg atgttgcatg gtttttgaac ggtaaattga 3900
atgcatcata caattgtgtt gacagacatg cctttgctaa tcccgacaag ccagctttga 3960
tctatgaagc tgatgacgaa tccgacaaca aaatcatcac atttggtgaa ttactcagaa 4020
aagtttccca aatcgctggt gtcttaaaaa gctggggcgt taagaaaggt gacacagtgg 4080
ctatctattt gccaatgatt ccagaagcgg tcattgctat gttggctgtg gctcgtattg 4140
gtgctattca ctctgttgtc tttgctgggt tctccgctgg ttcgttgaaa gatcgtgtcg 4200
ttgacgctaa ttctaaagtg gtcatcactt gtgatgaagg taaaagaggt ggtaagacca 4260
tcaacactaa aaaaattgtt gacgaaggtt tgaacggagt cgatttggtt tcccgtatct 4320
tggttttcca aagaactggt actgaaggta ttccaatgaa ggccggtaga gattactggt 4380
ggcatgagga ggccgctaag cagagaactt acctacctcc tgtttcatgt gacgctgaag 4440
atcctctatt tttattatac acttccggtt ccactggttc tccaaagggt gtcgttcaca 4500
ctacaggtgg ttatttatta ggtgccgctt taacaactag atacgttttt gatattcacc 4560
cagaagatgt tctcttcact gccggtgacg tcggctggat cacgggtcac acctatgctc 4620
tatatggtcc attaaccttg ggtaccgcct caataatttt cgaatccact cctgcctacc 4680
cagattatgg tagatattgg agaattatcc aacgtcacaa ggctacccat ttctatgtgg 4740
ctccaactgc tttaagatta atcaaacgtg taggtgaagc cgaaattgcc aaatatgaca 4800
cttcctcatt acgtgtcttg ggttccgtcg gtgaaccaat ctctccagac ttatgggaat 4860
ggtatcatga aaaagtgggt aacaaaaact gtgtcatttg tgacactatg tggcaaacag 4920
agtctggttc tcatttaatt gctcctttgg caggtgctgt cccaacaaaa cctggttctg 4980
ctaccgtgcc attctttggt attaacgctt gtatcattga ccctgttaca ggtgtggaat 5040
tagaaggtaa tgatgtcgaa ggtgtccttg ccgttaaatc accatggcca tcaatggcta 5100
gatctgtttg gaaccaccac gaccgttaca tggatactta cttgaaacct tatcctggtc 5160
actatttcac aggtgatggt gctggtagag atcatgatgg ttactactgg atcaggggta 5220
gagttgacga cgttgtaaat gtttccggtc atagattatc cacatcagaa attgaagcat 5280
ctatctcaaa tcacgaaaac gtctcggaag ctgctgttgt cggtattcca gatgaattga 5340
ccggtcaaac cgtcgttgca tatgtttccc taaaagatgg ttatctacaa aacaacgcta 5400
ctgaaggtga tgcagaacac atcacaccag ataatttacg tagagaattg atcttacaag 5460
ttaggggtga gattggtcct ttcgcctcac caaaaaccat tattctagtt agagatctac 5520
caagaacaag gtcaggaaag attatgagaa gagttctaag aaaggttgct tctaacgaag 5580
ccgaacagct aggtgaccta actactttgg ccaacccaga agttgtacct gccatcattt 5640
ctgctgtaga gaaccaattt ttctctcaaa aaaagaaata atttgccagc ttactatcct 5700
tcttgaaaat atgcactcta tatcttttag ttcttaattg caacacatag atttgctgta 5760
taacgaattt tatgctattt tttaaatttg gagttcagtg ataaaagtgt cacagcgaat 5820
ttcctcacat gtagggaccg aattgtttac aagttctctg taccaccatg gagacatcaa 5880
aaattgaaaa tctatggaaa gatatggacg gtagcaacaa gaatatagca cgagccgcgg 5940
agttcatttc gttacttttg atatcactca caactattgc gaagcgcttc agtgaaaaaa 6000
tcataaggaa aagttgtaaa tattattggt agtattcgtt tggtaaagta gagggggtaa 6060
tttttcccct ttattttgtt catacattct taaattgctt tgcctctcct tttggaaagc 6120
tatacttcgg agcactgttg agcgaaggct cattagatat attttctgtc attttcctta 6180
acccaaaaat aagggaaagg gtccaaaaag cgctcggaca actgttgacc gtgatccgaa 6240
ggactggcta tacagtgttc acaaaatagc caagctgaaa ataatgtgta gctatgttca 6300
gttagtttgg ctagcaaaga tataaaagca ggtcggaaat atttatgggc attattatgc 6360
agagcatcaa catgataaaa aaaaacagtt gaatattccc tcaaaaatga ctaagctaca 6420
ctttgacact gctgaaccag tcaagatcac acttccaaat ggtttgacat acgagcaacc 6480
aaccggtcta ttcattaaca acaagtttat gaaagctcaa gacggtaaga cctatcccgt 6540
cgaagatcct tccactgaaa acaccgtttg tgaggtctct tctgccacca ctgaagatgt 6600
tgaatatgct atcgaatgtg ccgaccgtgc tttccacgac actgaatggg ctacccaaga 6660
cccaagagaa agaggccgtc tactaagtaa gttggctgac gaattggaaa gccaaattga 6720
cttggtttct tccattgaag ctttggacaa tggtaaaact ttggccttag cccgtgggga 6780
tgttaccatt gcaatcaact gtctaagaga tgctgctgcc tatgccgaca aagtcaacgg 6840
tagaacaatc aacaccggtg acggctacat gaacttcacc accttagagc caatcggtgt 6900
ctgtggtcaa attattccat ggaactttcc aataatgatg ttggcttgga agatcgcccc 6960
agcattggcc atgggtaacg tctgtatctt gaaacccgct gctgtcacac ctttaaatgc 7020
cctatacttt gcttctttat gtaagaaggt tggtattcca gctggtgtcg tcaacatcgt 7080
tccaggtcct ggtagaactg ttggtgctgc tttgaccaac gacccaagaa tcagaaagct 7140
ggcttttacc ggttctacag aagtcggtaa gagtgttgct gtcgactctt ctgaatctaa 7200
cttgaagaaa atcactttgg aactaggtgg taagtccgcc catttggtct ttgacgatgc 7260
taacattaag aagactttac caaatctagt aaacggtatt ttcaagaacg ctggtcaaat 7320
ttgttcctct ggttctagaa tttacgttca agaaggtatt tacgacgaac tattggctgc 7380
tttcaaggct tacttggaaa ccgaaatcaa agttggtaat ccatttgaca aggctaactt 7440
ccaaggtgct atcactaacc gtcaacaatt cgacacaatt atgaactaca tcgatatcgg 7500
taagaaagaa ggcgccaaga tcttaactgg tggcgaaaaa gttggtgaca agggttactt 7560
catcagacca accgttttct acgatgttaa tgaagacatg agaattgtta aggaagaaat 7620
ttttggacca gttgtcactg tcgcaaagtt caagacttta gaagaaggtg tcgaaatggc 7680
taacagctct gaattcggtc taggttctgg tatcgaaaca gaatctttga gcacaggttt 7740
gaaggtggcc aagatgttga aggccggtac cgtctggatc aacacataca acgattttga 7800
ctccagagtt ccattcggtg gtgttaagca atctggttac ggtagagaaa tgggtgaaga 7860
agtctaccat gcatacactg aagtaaaagc tgtcagaatt aagttgtaaa aaaaaccccg 7920
gattctagaa ctagtggatc ccccgggctg caggaattcg atatcaagct tatcgatacc 7980
gtcgacctcg agtcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat 8040
ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt 8100
ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt 8160
acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac 8220
gctcgaaggc tttaatttgc tttggtcgtt tgctatgatt taattctgat tctttgcctt 8280
taacaattga taacgtgccc tttattgaac tgctaactac tcgaaaaatt ggacacaatt 8340
cagtttatag caggtgtcct ttaagtgatg tttgctgatt tccgaactgc gcgtatgtta 8400
gtggacccta gataacagtt tattccctaa cgtttctaga taatatatga caaaaatata 8460
caaccgatcg acaaattatt tctaaaatac aaaaaagtta gtcgtgaggt tatccaacgt 8520
tgggtttcta tctatatata aaagctaatc accaaacaat tgagaatcaa cgccgataac 8580
atgatgtaca attatagcag tatacgccag gcgtgctgca tttattcttt accctgtcat 8640
cgttgacgaa tacttttttt taaagggatc gcactctaac cacctgaaag gtactatctg 8700
aaaaagtgtt gactaaaaac agaagcgcct taataatagt gcactactgg aaaactatat 8760
ttaatgtaaa aaagaaacta cattgaatga aatcgaaaag tataatttct ctccgtacct 8820
gtgactgata caatgtaacg atgtagacta agaataagat tctcacttaa aagctgctat 8880
ttcaactgat tatctgcaga gtatgcgatc acaagacaga tcacaactgt gtttatcgga 8940
cataatgcta tatttttgct gcgccagaaa tcgagtacta gtttttaaat atttgttcta 9000
aacggtcaaa cttttcgtaa aaaatgctat aagccgtgtt gtccctttat gaagagctgc 9060
ttcttcactt gtaaatagcg aacaaataaa gaaaacaatc tgctacctct ttgttaaagt 9120
tgaccgcctt gaagagaact gcaagtctct gaaagtttta gctgaatatt gtcacctctt 9180
ttaggattaa cagtaagcat atttttttct ttgagattcg gaagtaaaat accctcggcg 9240
gtttaaacat cgtcgacctg caggcatgca agcttggcgt aatcatggtc atagctgttt 9300
cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag 9360
tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg 9420
cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg 9480
gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc 9540
tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 9600
acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 9660
aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 9720
cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 9780
gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 9840
tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 9900
tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 9960
cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 10020
gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 10080
ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 10140
ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 10200
ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 10260
agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 10320
aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 10380
atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 10440
tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 10500
tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 10560
tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 10620
gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 10680
tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 10740
ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 10800
gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 10860
aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 10920
ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 10980
tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 11040
ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 11100
aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 11160
ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 11220
ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 11280
agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt 11340
tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa 11400
ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt 11460
atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtc 11509
<210> 8
<211> 7257
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 8
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420
cctctagaga ttgtttaaac tatcatccac tgcacccact attgtgctgt cagaattagg 480
catcccagac ctcgtggtcg gcgcttccgg cggctcaaga atcactacga gtgttttaca 540
aacaattgtg aggacgtact ggtataacat gcctatattg gaaacgatag cctatccacg 600
tatacaccac cagttactgc ccgaccgcat agaactggaa agcttcccca tgatcggtaa 660
agccgtacta agcactctca aggaaatggg atatacgatg aaagaagtat ttcccaagag 720
cgtagttaat gctatacgca atgtcagggg agaatggcat gcggtgagtg actattggag 780
aaaaagagga atctcctccg tatactaaga tgtggggtaa gaatggtact gtaatatagt 840
tcaagataat agtacgtaat gtagggagcc tgcttctgtg gtatataaat aaatactggg 900
cttgcgttta tttttaaaga atcaatattt acttttcatt aacaaagggc atacgccctt 960
aaaacaaaac aaaaaatcca gatcatgcga gctttgcctt tttcagtaag tttccctacc 1020
ttgcagccga gacgcgactt cggaacgatg ccagcatacg tatttaccat tattaaaaaa 1080
atctgatggc ttgatactgc gcgtttcttt ttatagcatt aatacgcaca catacgcgca 1140
ttgttgttta ctataaataa accgtgggga cctgacccag tcatcctccc tgtgtttaca 1200
gtgcggtgca cacgagcctg agacaagccc gtaaccaggc gcgttgccgc aaatacagag 1260
gcgccccaga caacaccgca gtgtgaagca ctgtcaattt aaaaccgtgg cttgctggag 1320
atgcccagac caaccctgtt gggtttttct ctcgagcacg ccgttataat tttagcgtgt 1380
tccgtacctg tgtgcacatc aataagcggt gtaacaaact tgaacttgcc atctcatatc 1440
gtcatatgag cagttgcaga gaaaggcact ttaaataaaa aggcgtggat gataaaaaat 1500
gtatataagt tggatggatt tttgggaaaa agtaatgttt ttgcagacgt tttaaatact 1560
ccctcccttt tcttagtaat ttttattatg tattgactaa gtcaaaaata actatagaaa 1620
actaaagttt acgagaggac ccaaaagttt tgaataacac gtgcctttga ttttttgttt 1680
actttctttt tctagttaat taccaactaa acggattaga agccgccgag cgggtgacag 1740
ccctccgaag gaagactctc ctccgtgcgt cctcgtcttc accggtcgcg ttcctgaaac 1800
gcagatgtgc ctcgcgccgc actgctccga acaataaaga ttctacaata ctagctttta 1860
tggttatgaa gaggaaaaat tggcagtaac ctggccccac aaaccttcaa atgaacgaat 1920
caaattaaca accataggat gataatgcga ttagtttttt agccttattt ctggggtaat 1980
taatcagcga agcgatgatt tttgatctat taacagatat ataaatgcaa aaactgcata 2040
accactttaa ctaatacttt caacattttc ggtttgtatt acttcttatt caaatgtaat 2100
aaaagtatca acaaaaaatt gttaatatac ctctatactt taacgtcaag gagatgtttg 2160
tcagggttaa attgaataaa ccagtaaaat ggtataggtt ctatagtacg ttggattcac 2220
attccctaaa gttacagagc ggctcgaagt ttgtaaaaat aaagccagta aataacttga 2280
ggagtagttc atcagcagat ttcgtgtccc caccaaattc caaattacaa tctttaatct 2340
ggcagaaccc tttacaaaat gtttatataa ctaaaaaacc atggactcca tccacaagag 2400
aagcgatggt tgaattcata actcatttac atgagtcata ccccgaggtg aacgtcattg 2460
ttcaacccga tgtggcagaa gaaatttccc aggatttcaa atctcctttg gagaatgatc 2520
ccaaccgacc tcatatactt tatactggtc ctgaacaaga tatcgtaaac agaacagact 2580
tattggtgac attgggaggt gatgggacta ttttacacgg cgtatcaatg ttcggaaata 2640
cgcaagttcc tccggtttta gcatttgctc tgggcactct gggctttcta ttaccgtttg 2700
attttaagga gcataaaaag gtctttcagg aagtaatcag ctctagagcc aaatgtttgc 2760
atagaacacg gctagaatgt catttgaaaa aaaaggatag caactcatct attgtgaccc 2820
atgctatgaa tgacatattc ttacataggg gtaattcccc tcatctcact aacctggaca 2880
ttttcattga tggggaattt ttgacaagaa cgacagcaga tggtgttgca ttggccactc 2940
caacgggttc cacagcatat tcattatcag caggtggatc tattgtttcc ccattagtcc 3000
ctgctatttt aatgacacca atttgtcctc gctctttgtc attccgacca ctgattttgc 3060
ctcattcatc ccacattagg ataaagatag gttccaaatt gaaccaaaaa ccagtcaaca 3120
gtgtggtaaa actttctgtt gatggtattc ctcaacagga tttagatgtt ggtgatgaaa 3180
tttatgttat aaatgaggtc ggcactatat acatagatgg tactcagctt ccgacgacaa 3240
gaaaaactga aaatgacttt aataattcaa aaaagcctaa aaggtcaggg atttattgtg 3300
tcgccaagac cgagaatgac tggattagag gaatcaatga acttttagga ttcaattcta 3360
gctttaggct gaccaagaga cagactgata atgattaaaa aaaaccccgg attctagaac 3420
tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga 3480
gtcatgtaat tagttatgtc acgcttacat tcacgccctc cccccacatc cgctctaacc 3540
gaaaaggaag gagttagaca acctgaagtc taggtcccta tttatttttt tatagttatg 3600
ttagtattaa gaacgttatt tatatttcaa atttttcttt tttttctgta cagacgcgtg 3660
tacgcatgta acattatact gaaaaccttg cttgagaagg ttttgggacg ctcgaaggct 3720
ttaatttgcg tcgccctcag tccgctcatt ttagctgaat tttctaatgt tatttttcat 3780
cagcaaaact taacagaacg ttaatttatc tacccctttt agttcattat ctctttttta 3840
tccaacattt tacagagatc tctcacttaa gtctaagtaa agacattatt ttatatggta 3900
cacttataga atatacgata ataataataa aaactatgta acataacctt cagaatttaa 3960
tattagtttc ctttttacct cattgcacta ataaaaaaat tctacagaat ctccgaaaaa 4020
gaaaatccag cttactcttt ttgttttctt cttcacacgt gagcttttcc gccggcatac 4080
gttccgttcc gtgtcgtctt gcataaaatt tccgaatcac atgttcgtaa aacaaccgga 4140
agtgccccga atataaagtc aattctcacc gctgttgtaa ctggagcttt aaggtgttat 4200
ctaaggaagg ataaaagaac ttaaacaaca acaaacaaaa aattaagtta caatgccagc 4260
attattaaaa agattattgt ttcaagtggg tcctcatcca aacgaaagaa cattcacctt 4320
atcctctgtt tcaactgatg ggcactatat ttccttgaga ccatttgtta agccaagcgg 4380
tgatgagtta tcttttcctt tcgaatgggc ctttgccggt acaaacgaaa cagttaaagc 4440
taatgatcaa ggaaacggtg tcgttactca agatttcaat ttctggttgg atacaaatgt 4500
gtacttgaac gttccaaaca cccatcgtgg cgaagtgaac accacttgga aaaattggga 4560
ttctggttgt gtcgaggaaa caggcgctgt ttacccattc ggtgccgaca aagaaagcgt 4620
ctctttcaga gaattgtggc aaccagttga cccatcaaga gaagatctag tcatcgtctc 4680
accaaacaat gagaagttct cgtcgaatgc taggtcaatt gtcctcaaag ttactgacga 4740
agcttatgat ggtttggtta ttgttattgg tagatggatt caagggtttt tgtcccaaaa 4800
gaataataac actattgaag gcttgaactt catcagatta cttgaaaaag attcaggtaa 4860
atctgagttc ttattaagct acggtaagga agtaaacaaa attccacaaa gctacgaaaa 4920
tttgaagaaa ggttccactg taaccagcaa tgggttgaac tgggaagtta ttgaatatca 4980
cgcttaatgt ttaaacatcg tcgacctgca ggcatgcaag cttggcgtaa tcatggtcat 5040
agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa 5100
gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc 5160
gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc 5220
aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact 5280
cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac 5340
ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa 5400
aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg 5460
acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa 5520
gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc 5580
ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac 5640
gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac 5700
cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg 5760
taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt 5820
atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa 5880
cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 5940
cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 6000
ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 6060
ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 6120
tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 6180
aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 6240
tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 6300
gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 6360
atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 6420
tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 6480
ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 6540
ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 6600
tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 6660
ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 6720
ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 6780
tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 6840
gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 6900
taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 6960
cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 7020
agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 7080
gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 7140
ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 7200
ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc 7257
<210> 9
<211> 6581
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 9
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420
cctctagaga ttgtttaaac tttcctctaa tcaggttcca ccaaacagat accccggtgt 480
ttcacggaat ggtacgtttg atatcgctga tttgagagga ggttacactt gaagaatcac 540
agtcttgcga ccggctattc aacaaggcat tcccccaagt ttgaattctt tgaaatagat 600
tgctattagc tagtaatcca ccaaatcctt cgctgctcac caatggaatc gcaagatgcc 660
cacgatgaga ctgttcaggt taaacgcaaa agaaacacac tctgggaatt tcttcccaaa 720
ttgtatctct caatacgcat caacccatgt caattaaaca cgctgtatag agactaggca 780
gatctgacga tcacctagcg actctctcca ccgtttgacg aggccattta caaaaacata 840
acgaacgaca agcctactcg aattcgtttc caaactcttt tcgaacttgt cttcaactgc 900
tttcgcatga agtacctccc aactactttt cctcacactt gtactccatg actaaacccc 960
ccctcccatt acaaactaaa atcttacttt tattttcttt tgccctctct gtcgctctgc 1020
cttaactacg tatttctcgc cgagaaaaac ttcaatttaa gctattctcc aaaaatctta 1080
gcgtatattt tttttccaaa gtgatgcagg tctcatctgg aatataattc ccccctcctg 1140
aagcaaattt ttcctttgag ccggaatttt tgatattccg agttcttttt ttccattcgc 1200
ggaggttatt ccattcctaa acgagtggcc acaatgaaac ttcaattcat atcgaccgac 1260
tatttttctc cgaaccaaaa aaatagcagg gcgagattgg agctgcggaa aaaagaggaa 1320
aaaatttttt cgtagttttc ttgtgcaaat tagggtgtaa ggtttctagg gcttattggt 1380
tcaagcagaa gagacaacaa ttgtaggtcc taaattcaag gcggatgtaa ggagtattgg 1440
tttcgaaagt ttttccgaag cggcatggca gggactactt gcgcatgcgc tcggattatc 1500
ttcatttttg cttgcaaaaa cgtagaatca tggtaaatta catgaagaat tctctttttt 1560
tttttttttt tttttttttt acctctaaag agtgttgacc aactgaaaaa acccttcttc 1620
aagagagtta aactaagact aaccatcata acttccaagg aattaatcga tatcttgcac 1680
tcctgatttt tcttcaaaga gacagcgcaa aggattatga cactgttgca ttgagtcaaa 1740
agtttttccg aagtgaccca gtgctctttt tttttttccg tgaaggactg acaaatatgc 1800
gcacaagatc caatacgtaa tggaaattcg gaaaaactag gaagaaatgc tgcagggcat 1860
tgccgtgccg atcttttgtc tttcagatat atgagaaaaa gaatattcat caagtgctga 1920
tagaagaata ccactcatat gacgtgggca gaagacagca aacgtaaaca tgagctgctg 1980
cgacatttga tggcttttat ccgacaagcc aggaaactcc accattatct aatgtagcaa 2040
aatatttctt aacacccgaa gttgcgtgtc cccctcacgt ttttaatcat ttgaattagt 2100
atattgaaat tatatataaa ggcaacaatg tccccataat caattccatc tggggtctca 2160
tgttctttcc ccaccttaaa atctataaag atatcataat cgtcaactag ttgatatacg 2220
taaaatcatg ggaaagctat tacaattggc attgcatccg gtcgagatga aggcagcttt 2280
gaagctgaag ttttgcagaa caccgctatt ctccatctat gatcagtcca cgtctccata 2340
tctcttgcac tgtttcgaac tgttgaactt gacctccaga tcgtttgctg ctgtgatcag 2400
agagctgcat ccagaattga gaaactgtgt tactctcttt tatttgattt taagggcttt 2460
ggataccatc gaagacgata tgtccatcga acacgatttg aaaattgact tgttgcgtca 2520
cttccacgag aaattgttgt taactaaatg gagtttcgac ggaaatgccc ccgatgtgaa 2580
ggacagagcc gttttgacag atttcgaatc gattcttatt gaattccaca aattgaaacc 2640
agaatatcaa gaagtcatca aggagatcac cgagaaaatg ggtaatggta tggccgacta 2700
catcttagat gaaaattaca acttgaatgg gttgcaaacc gtccacgact acgacgtgta 2760
ctgtcactac gtagctggtt tggtcggtga tggtttgacc cgtttgattg tcattgccaa 2820
gtttgccaac gaatctttgt attctaatga gcaattgtat gaaagcatgg gtcttttcct 2880
acaaaaaacc aacatcatca gagattacaa tgaagatttg gtcgatggta gatccttctg 2940
gcccaaggaa atctggtcac aatacgctcc tcagttgaag gacttcatga aacctgaaaa 3000
cgaacaactg gggttggact gtataaacca cctcgtctta aacgcattga gtcatgttat 3060
cgatgtgttg acttatttgg ccggtatcca cgagcaatcc actttccaat tttgtgccat 3120
tccccaagtt atggccattg caaccttggc tttggtattc aacaaccgtg aagtgctaca 3180
tggcaatgta aagattcgta agggtactac ctgctattta attttgaaat caaggacttt 3240
gcgtggctgt gtcgagattt ttgactatta cttacgtgat atcaaatcta aattggctgt 3300
gcaagatcca aatttcttaa aattgaacat tcaaatctcc aagatcgaac agtttatgga 3360
agaaatgtac caggataaat tacctcctaa cgtgaagcca aatgaaactc caattttctt 3420
gaaagttaaa gaaagatcca gatacgatga tgaattggtt ccaacccaac aagaagaaga 3480
gtacaagttc aatatggttt tatctatcat cttgtccgtt cttcttgggt tttattatat 3540
atacacttta cacagagcgt gaaactaacg ctaatcgata aaacattaga tttcaaacta 3600
gataaggacc atgtataaga actatatact tccaatataa tatagtataa gctttaagat 3660
agtatctctc gatctaccgt tccacgtgac tagtccaagg atttttttta acaggtgccc 3720
cgggtaaccc agttcctcac tattttttac tgcggaagcg gaagcggaaa atacggaaac 3780
gcgcgggaac atacaaaaca tacaaaatat acctttctca cacaagaaat atatgctact 3840
tgcaaaatat cataccaaaa aacttttcac aaccgaaacc aaaaccaacg gatatcatac 3900
attacactac caccattcaa actttactac tatcctccct tcagtttccc tttttctgcc 3960
tttttcggtg acggaaatac gcttcagaga ccctaaaggg aaatccatgc cataacagga 4020
aagtaacatc ccaatgcgga ctataccacc ccaccacact cctaccaata acggtaacta 4080
ttctatgttt tcttactcct atgtctattc atctttcatc tgactaccta atactatgca 4140
aaaatgtaaa atcatcacac aaaacataaa caatcaaaat cagccatttc cgcacctttt 4200
cctctgtcca ctttcaaccg tccctccaaa tgtaaaatgg cctatcggaa tacattttct 4260
acatcctaac tactataaaa caacctttag acttacgttt gctactctca tggtttaaac 4320
atcgtcgacc tgcaggcatg caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg 4380
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 4440
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 4500
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 4560
cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 4620
tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 4680
aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 4740
aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 4800
tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 4860
ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 4920
cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 4980
ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 5040
ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 5100
gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 5160
agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg 5220
cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 5280
aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 5340
aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 5400
ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 5460
aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 5520
ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 5580
agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 5640
cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 5700
ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 5760
gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 5820
cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 5880
cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 5940
ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 6000
catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 6060
tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 6120
ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct 6180
catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 6240
cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 6300
cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 6360
acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 6420
ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 6480
tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 6540
attaacctat aaaaataggc gtatcacgag gccctttcgt c 6581
<210> 10
<211> 9748
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 10
tgatccaata tcaaaggaaa tgatagcatt gaaggatgag actaatccaa ttgaggagtg 60
gcagcatata gaacagctaa agggtagtgc tgaaggaagc atacgatacc ccgcatggaa 120
tgggataata tcacaggagg tactagacta cctttcatcc tacataaata gacgcatata 180
agtacgcatt taagcataaa cacgcactat gccgttcttc tcatgtatat atatatacag 240
gcaacacgca gatataggtg cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc 300
attttcggaa gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa gttcctattc 360
tctagaaagt ataggaactt cagagcgctt ttgaaaacca aaagcgctct gaagacgcac 420
tttcaaaaaa ccaaaaacgc accggactgt aacgagctac taaaatattg cgaataccgc 480
ttccacaaac attgctcaaa agtatctctt tgctatatat ctctgtgcta tatccctata 540
taacctaccc atccaccttt cgctccttga acttgcatct aaactcgacc tctacatttt 600
ttatgtttat ctctagtatt actctttaga caaaaaaatt gtagtaagaa ctattcatag 660
agtgaatcga aaacaatacg aaaatgtaaa catttcctat acgtagtata tagagacaaa 720
atagaagaaa ccgttcataa ttttctgacc aatgaagaat catcaacgct atcactttct 780
gttcacaaag tatgcgcaat ccacatcggt atagaatata atcggggatg cctttatctt 840
gaaaaaatgc acccgcagct tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt 900
tttttatgga agagaaaata gacaccaaag tagccttctt ctaaccttaa cggacctaca 960
gtgcaaaaag ttatcaagag actgcattat agagcgcaca aaggagaaaa aaagtaatct 1020
aagatgcttt gttagaaaaa tagcgctctc gggatgcatt tttgtagaac aaaaaagaag 1080
tatagattct ttgttggtaa aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc 1140
agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa 1200
tgaagcacag attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa 1260
aatgcagctc agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttctac 1320
aaaatgaagc acagatgctt cgttcaggtg gcacttttcg gggaaatgtg cgcggaaccc 1380
ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 1440
gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 1500
cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 1560
tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 1620
tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 1680
cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 1740
tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 1800
agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 1860
ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 1920
ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 1980
aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 2040
gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 2100
tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 2160
ttgctgataa atctggagcc ggtgagcgtg ggagccgcgg tatcattgca gcactggggc 2220
cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 2280
atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 2340
cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 2400
ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 2460
cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 2520
ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 2580
tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 2640
taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 2700
caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 2760
agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 2820
gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 2880
gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 2940
ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 3000
acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 3060
tgtgatgctc gtcagggggg cggagcctat ggaaagcggc cgcacaatct ttgaaaagat 3120
aatgtatgat tatgctttca ctcatattta tacagaaact tgatgttttc tttcgagtat 3180
atacaaggtg attacatgta cgtttgaagt acaactctag attttgtagt gccctcttgg 3240
gctagcggta aaggtgcgca ttttttcaca ccctacaatg ttctgttcaa aagattttgg 3300
tcaaacgctg tagaagtgaa agttggtgcg catgtttcgg cgttcgaaac ttctccgcag 3360
tgaaagataa atgatcatca atggtttgaa cggaaggttt tagagctaga aatagcaagt 3420
taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcaaacaagc 3480
gcaagtggtt tagtggtaaa atccaacgtt gccatcgttg ggcccccggt tcgattccgg 3540
gcttgcgcac atattgaaaa aatactctgg ttttagagct agaaatagca agttaaaata 3600
aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttcttttttg 3660
tccaccacca aaatgtcaat ttttttgcca ttttctaaat agttttcttc actctacaag 3720
agatatcacg attgtttgct ttcgatattg gatccatcta aagtcatttc aactatctaa 3780
gatgtggttg tgattgggcc attttgtgaa agccagtacg ccagcgtcaa tacactcccg 3840
tcaattagtt gcaccatgtc cacaaaatca tataccagta gagctgagac tcatgcaagt 3900
ccggttgcat cgaaactttt acgtttaatg gatgaaaaga agaccaattt gtgtgcttct 3960
cttgacgttc gttcgactga tgagctattg aaacttgttg aaacgttggg tccatacatt 4020
tgccttttga aaacacacgt tgatatcttg gatgatttca gttatgaggg tactgtcgtt 4080
ccattgaaag cattggcaga gaaatacaag ttcttgatat ttgaggacag aaaattcgcc 4140
gatatcggta acacagtcaa attacaatat acatcgggcg tttaccgtat cgcagaatgg 4200
tctgatatca ccaacgccca cggggttact ggtgctggta ttgttgctgg cttgaaacaa 4260
ggtgcgcaag aggtcaccaa agaaccaagg ggattattga tgcttgctga attgtcttcc 4320
aagggttctc tagcacacgg tgaatatact aagggtaccg ttgatattgc aaagagtgat 4380
aaagatttcg ttattgggtt cattgctcag aacgatatgg gaggaagaga agaagggttt 4440
gattggctaa tcatgacccc aggtgtaggt ttagacgaca aaggcgatgc attgggtcag 4500
cagtacagaa ccgtcgacga agttgtaagt ggtggatcag atatcatcat tgttggcaga 4560
ggacttttcg ccaagggtag agatcctaag gttgaaggtg aaagatacag aaatgctgga 4620
tgggaagcgt accaaaagag aatcagcgct ccccattaat tatacaggaa acttaataga 4680
acaaatcaca tatttaatct aatagccacc tgcattggca cggtgcaaca ctacttcaac 4740
ttcatcttac aaaaagatca cgtgatctgt tgtattgaac tgaaaatttt ttgtttgctt 4800
ctctctctct ctctttcatt atgtgagatt taaaaaccag aaactacatc atcgaaaaag 4860
tctagagttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga 4920
aaaagtggca ccgagtcggt gcttttttta ttttttgtca ctattgcatg ccggtagagg 4980
tgtggtcaat aagagcgacc tcatgctata cctgagaaag caacctgacc tacaggaaag 5040
agttactcaa gaataagaat tttcgtttta aaacctaaga gtcactttaa aatttgtata 5100
cacttatttt ttttataact tatttaataa taaaaatcat aaatcataag aaattcgcct 5160
cgagtcagac ctttctcttc ttttttggag ggtcacctcc tagctgactc aaatcaatgc 5220
gtgtttcata aagaccagtg atggattgat ggataagagt ggcatctaaa acttcttttg 5280
tagacgtata tcgtttacga tcaattgttg tatcaaaata tttaaaagca gcgggagctc 5340
caagattcgt caacgtaaat aaatgaataa tattttctgc ttgttcacgt attggtttgt 5400
ctctatgttt gttatatgca ctaagaactt tatctaaatt ggcatctgct aaaataacac 5460
gcttagaaaa ttcactgatt tgctcaataa tctcatctaa ataatgctta tgctgctcca 5520
caaacaattg tttttgttcg ttatcttctg gactaccctt caacttttca taatgactag 5580
ctaaatataa aaaattcaca tatttgcttg gcagagccag ctcatttcct ttttgtaatt 5640
ctccggcact agccagcatc cgtttacgac cgttttctaa ctcaaaaaga ctatatttag 5700
gtagtttaat gattaagtct tttttaactt ccttatatcc tttagcttct aaaaagtcaa 5760
tcggattttt ttcaaaggaa cttctttcca taattgtgat ccctagtaac tctttaacgg 5820
attttaactt cttcgatttc cctttttcca ccttagcaac cactaggact gaataagcta 5880
ccgttggact atcaaaacca ccatattttt ttggatccca gtctttttta cgagcaataa 5940
gcttgtccga atttcttttt ggtaaaattg actccttgga gaatccgcct gtctgtactt 6000
ctgttttctt gacaatattg acttggggca tggacaatac tttgcgcact gtggcaaaat 6060
ctcgcccttt atcccagaca atttctccag tttccccatt agtttcgatt agagggcgtt 6120
tgcgaatctc tccatttgca agtgtaattt ctgttttgaa gaagttcatg atattagagt 6180
aaaagaaata ttttgcggtt gctttgccta tttcttgctc agacttagca atcattttac 6240
gaacatcata aactttataa tcaccataga caaactccga ttcaagtttt ggatatttct 6300
taatcaaagc agttccaacg acggcattta gatacgcatc atgggcatga tggtaattgt 6360
taatctcacg tactttatag aattggaaat cttttcggaa gtcagaaact aatttagatt 6420
ttaaggtaat cactttaacc tctcgaataa gtttatcatt ttcatcgtat ttagtattca 6480
tgcgactatc caaaatttgt gccacatgct tagtgatttg gcgagtttca accaattggc 6540
gtttgataaa accagcttta tcaagttcac tcaaacctcc acgttcagct ttcgttaaat 6600
tatcaaactt acgttgagtg attaacttgg cgtttagaag ttgtctccaa tagtttttca 6660
tctttttgac tacttcttca cttggaacgt tatccgattt accacgattt ttatcagaac 6720
gcgttaagac cttattgtct attgaatcgt ctttaaggaa actttgtgga acaatgtgat 6780
cgacatcata atcacttaaa cgattaatat ctaattcttg gtccacatac atgtctcttc 6840
cattttggag ataatagaga tagagctttt cattttgcaa ttgagtattt tcaacaggat 6900
gctctttaag aatctgactt cctaattctt tgataccttc ttcgattcgt ttcatacgct 6960
ctcgcgaatt tttctggccc ttttgagttg tctgattttc acgtgccatt tcaataacga 7020
tattttctgg cttatgccgc cccattactt tgaccaattc atcaacaact tttacagtct 7080
gtaaaatacc ttttttaata gcagggctac cagctaaatt tgcaatatgt tcatgtaaac 7140
tatcgccttg tccagacact tgtgcttttt gaatgtcttc tttaaatgtc aaactatcat 7200
catggatcag ctgcataaaa ttgcgattgg caaaaccatc tgatttcaaa aaatctaata 7260
ttgttttgcc agattgctta tccctaatac cattaatcaa ttttcgagac aaacgtcccc 7320
aaccagtata acggcgacgt ttaagctgtt tcatcacctt atcatcaaag aggtgagcat 7380
atgttttaag tctttcctca atcatctccc tatcttcaaa taaggtcaat gttaaaacaa 7440
tatcctctaa gatatcttca ttttcttcat tatccaaaaa atctttatct ttaataattt 7500
ttagcaaatc atggtaggta cctaatgaag cattaaatct atcttcaact cctgaaattt 7560
caacactatc aaaacattct atttttttga aataatcttc ttttaattgc ttaacggtta 7620
cttttcgatt tgttttgaag agtaaatcaa caatggcttt cttctgttca cctgaaagaa 7680
atgctggttt tcgcattcct tcagtaacat atttgacctt tgtcaattcg ttataaaccg 7740
taaaatactc ataaagcaaa ctatgttttg gtagtacttt ttcatttgga agatttttat 7800
caaagtttgt catgcgttca ataaatgatt gagctgaagc acctttatcg acaacttctt 7860
caaaattcca tggggtaatt gtttcttcag acttccgagt catccatgca aaacgactat 7920
tgccacgcgc caatggacca acataataag gaattcgaaa agtcaagatt ttttcaatct 7980
tctcacgatt gtcttttaaa aatggataaa agtcttcttg tcttctcaaa atagcatgca 8040
gctcacccaa gtgaatttga tgggtaatag agccgttgtc aaaggtccgt tgcttgcgca 8100
gcaaatcttc acgatttagt ttcaccaata attcctcagt accatccatt ttttctaaaa 8160
ttggtttgat aaatttataa aattcttctt ggctagctcc cccatcaata taacctgcat 8220
atccgttttt tgattgatca aaaaagattt ctttatactt ttctggaagt tgttgtcgaa 8280
ctaaagcttt taaaagagtc aagtcttgat gatgttcatc gtagcgttta atcattgaag 8340
ctgatagggg agccttagtt atttcagtat ttactcttag gatatctgaa agtaaaatag 8400
catctgataa attcttagct gccaaaaaca aatcagcata ttgatctcca atttgcgcca 8460
ataaattatc taaatcatca tcgtaagtat cttttgaaag ctgtaattta gcatcttctg 8520
ccaaatcaaa atttgattta aaattagggg tcaaacccaa tgacaaagca atgagattcc 8580
caaataagcc atttttcttc tcaccgggga gctgagcaat gagattttct aatcgtcttg 8640
atttactcaa tcgtgcagaa agaatcgctt tagcatctac tccacttgcg ttaatagggt 8700
tttcttcaaa taattgattg taggtttgta ccaactggat aaatagtttg tccacatcac 8760
tattatcagg atttaaatct ccctcaatca aaaaatgacc acgaaactta atcatatgcg 8820
ctaaggccaa atagattaag cgcaaatccg ctttataagt agaatctacc aatttttttc 8880
gcagatgata gatagttgga tatttctcat gataagcaac ttcatctact atatttccaa 8940
aaataggatg acgttcatgc ttcttgtctt cttccaccaa aaaagactct tcaagtcgat 9000
gaaagaaact atcatctact ttcgccatct catttgaaaa aatctcctgt agataacaaa 9060
tacgattctt ccgacgtgta taccttctac gagctgtccg tttgagacga gtcgcttccg 9120
ctgtctctcc actgtcaaat aaaagagccc ctataagatt ttttttgata ctgtggcggt 9180
ctgtatttcc cagaaccttg aactttttag acggaacctt atattcatca gtgatcaccg 9240
cccatccgac gctatttgtg ccgatatcta agcctattga gtatttctta tcgacctttc 9300
tcttcttttt tggaggtttg tcatcgtcat ctttataatc catctagaaa acttagatta 9360
gattgctatg ctttctttct aatgagcaag aagtaaaaaa agttgtaata gaacaagaaa 9420
aatgaaactg aaacttgaga aattgaagac cgtttattaa cttaaatatc aatgggaggt 9480
catcgaaaga gaaaaaaatc aaaaaaaaaa ttttcaagaa aaagaaacgt gataaaaatt 9540
tttattgcct ttttcgacga agaaaaagaa acgaggcggc ctcttttttc ttttccaaac 9600
ctttagtacg ggtaattaac gacaccctag aggaagaaag aggggaaatt tagtatgctg 9660
tgcttgggtg ttttgaagtg gtacggcgat gcgcggagtc cgagaaaatc tggaagagta 9720
aaaaaggagt agaaacattt tgaagatc 9748
<210> 11
<211> 5173
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 11
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420
cctctagaga ttgtttaaac tcaggagaac ttccctgtgc atacgggaca gcagagatag 480
gctgttctcg cgcgtttttt tttttttttt gctttttttg gttcctttcg cggatatcgt 540
attatcgata ttacaacaaa aatgtttgga gatctccttc gctgtcttta agtaaaaggc 600
tttaaccgct tatttttctc tctttttttc cttttacccc tcacttgtcg tgagacaatt 660
gtagtatatt tagtaagtat agaaagttct tgttgttaaa aaactcgtta ggatacaata 720
aaaactagaa caaacacaaa agacaaaaaa agacaacaat tgcaggtctc atctggaata 780
taattccccc ctcctgaagc aaatttttcc tttgagccgg aatttttgat attccgagtt 840
ctttttttcc attcgcggag gttattccat tcctaaacga gtggccacaa tgaaacttca 900
attcatatcg accgactatt tttctccgaa ccaaaaaaat agcagggcga gattggagct 960
gcggaaaaaa gaggaaaaaa ttttttcgta gttttcttgt gcaaattagg gtgtaaggtt 1020
tctagggctt attggttcaa gcagaagaga caacaattgt aggtcctaaa ttcaaggcgg 1080
atgtaaggag tattggtttc gaaagttttt ccgaagcggc atggcaggga ctacttgcgc 1140
atgcgctcgg attatcttca tttttgcttg caaaaacgta gaatcatggt aaattacatg 1200
aagaattctc tttttttttt tttttttttt ttttttacct ctaaagagtg ttgaccaact 1260
gaaaaaaccc ttcttcaaga gagttaaact aagactaacc atcataactt ccaaggaatt 1320
aatcgatatc ttgcactcct gatttttctt caaagagaca gcgcaaagga ttatgacact 1380
gttgcattga gtcaaaagtt tttccgaagt gacccagtgc tctttttttt tttccgtgaa 1440
ggactgacaa atatgcgcac aagatccaat acgtaatgga aattcggaaa aactaggaag 1500
aaatgctgca gggcattgcc gtgccgatct tttgtctttc agatatatga gaaaaagaat 1560
attcatcaag tgctgataga agaataccac tcatatgacg tgggcagaag acagcaaacg 1620
taaacatgag ctgctgcgac atttgatggc ttttatccga caagccagga aactccacca 1680
ttatctaatg tagcaaaata tttcttaaca cccgaagttg cgtgtccccc tcacgttttt 1740
aatcatttga attagtatat tgaaattata tataaaggca acaatgtccc cataatcaat 1800
tccatctggg gtctcatgtt ctttccccac cttaaaatct ataaagatat cataatcgtc 1860
aactagttga tatacgtaaa atcatggcgg acacgttatt gattctgggt gatagcctga 1920
gcgccgggta tcgaatgtct gccagcgcgg cctggcctgc cttgttgaat gataagtggc 1980
agagtaaaac gtcggtagtt aatgccagca tcagcggcga cacctcgcaa caaggactgg 2040
cgcgccttcc ggctctgctg aaacagcatc agccgcgttg ggtgctggtt gaactgggcg 2100
gcaatgacgg tttgcgtggt tttcagccac agcaaaccga gcaaacgctg cgccagattt 2160
tgcaggatgt caaagccgcc aacgctgaac cattgttaat gcaaatacgt ctgcctgcaa 2220
actatggtcg ccgttataat gaagccttta gcgccattta ccccaaactc gccaaagagt 2280
ttgatgttcc gctgctgccc ttttttatgg aagaggtcta cctcaagcca caatggatgc 2340
aggatgacgg tattcatccc aaccgcgacg cccagccgtt tattgccgac tggatggcga 2400
agcagttgca gcctttagta aatcatgact cataaaacta acgctaatcg ataaaacatt 2460
agatttcaaa ctagataagg accatgtata agaactatat acttccaata taatatagta 2520
taagctttaa gatagtatct ctcgatctac cgttccacgt gactagtcca aggatttttt 2580
ttaatggatc aacatttcca tgataggaaa gcctcatcat actaaagcac tttttcagtt 2640
ttttgcttta gaactgctac caattataaa atagaatgca caggttatct tgtttggttt 2700
gttattatcc ttttttcttc tttaccagtc atatcacctc attcacgtat gttcgtaatt 2760
tataatgtta aaaatggcat atatagatat atttatgaac atcctaaaaa ataactaaat 2820
ttttctacat ttcaaaaaag gtaacgttga ctgccgcatg gcagtctaat taggagctta 2880
gtgatacata tatatatata tatagtttaa acatcgtcga cctgcaggca tgcaagcttg 2940
gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 3000
aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 3060
acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 3120
cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 3180
tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 3240
tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 3300
gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 3360
aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 3420
ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 3480
gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 3540
ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 3600
ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 3660
cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 3720
attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 3780
ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 3840
aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 3900
gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 3960
tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 4020
ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 4080
taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 4140
atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 4200
actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 4260
cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 4320
agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 4380
gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 4440
gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 4500
gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 4560
gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 4620
cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 4680
ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 4740
accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 4800
aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 4860
aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 4920
caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 4980
ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 5040
gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 5100
cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 5160
aggccctttc gtc 5173
<210> 12
<211> 11283
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 12
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420
cctctagaga ttgtttaaac ctcggccttc gttcatctcg ttctttctac tcttagccct 480
catctttctc ttctgcattc ttttcacctc attaagaaca ttcgcggcgc cgctttaccc 540
caacaaaaac agcccatctt ttgttctacc tctttcagat aaggctcaac agatagacaa 600
taactgatca ttctttcttt tgtttgcaac ccctctgagt tgacataaaa gcgagaatac 660
aaaagaacca gcattagtaa cacatcattt ttttctctgt tcttcactat ttcttgaaaa 720
actaagaagt acgcatcaaa tgcaggtctc atctggaata taattccccc ctcctgaagc 780
aaatttttcc tttgagccgg aatttttgat attccgagtt ctttttttcc attcgcggag 840
gttattccat tcctaaacga gtggccacaa tgaaacttca attcatatcg accgactatt 900
tttctccgaa ccaaaaaaat agcagggcga gattggagct gcggaaaaaa gaggaaaaaa 960
ttttttcgta gttttcttgt gcaaattagg gtgtaaggtt tctagggctt attggttcaa 1020
gcagaagaga caacaattgt aggtcctaaa ttcaaggcgg atgtaaggag tattggtttc 1080
gaaagttttt ccgaagcggc atggcaggga ctacttgcgc atgcgctcgg attatcttca 1140
tttttgcttg caaaaacgta gaatcatggt aaattacatg aagaattctc tttttttttt 1200
tttttttttt ttttttacct ctaaagagtg ttgaccaact gaaaaaaccc ttcttcaaga 1260
gagttaaact aagactaacc atcataactt ccaaggaatt aatcgatatc ttgcactcct 1320
gatttttctt caaagagaca gcgcaaagga ttatgacact gttgcattga gtcaaaagtt 1380
tttccgaagt gacccagtgc tctttttttt tttccgtgaa ggactgacaa atatgcgcac 1440
aagatccaat acgtaatgga aattcggaaa aactaggaag aaatgctgca gggcattgcc 1500
gtgccgatct tttgtctttc agatatatga gaaaaagaat attcatcaag tgctgataga 1560
agaataccac tcatatgacg tgggcagaag acagcaaacg taaacatgag ctgctgcgac 1620
atttgatggc ttttatccga caagccagga aactccacca ttatctaatg tagcaaaata 1680
tttcttaaca cccgaagttg cgtgtccccc tcacgttttt aatcatttga attagtatat 1740
tgaaattata tataaaggca acaatgtccc cataatcaat tccatctggg gtctcatgtt 1800
ctttccccac cttaaaatct ataaagatat cataatcgtc aactagttga tatacgtaaa 1860
atcatgagcg aagaaagctt attcgagtct tctccacaga agatggagta cgaaattaca 1920
aactactcag aaagacatac agaacttcca ggtcatttca ttggcctcaa tacagtagat 1980
aaactagagg agtccccgtt aagggacttt gttaagagtc acggtggtca cacggtcata 2040
tccaagatcc tgatagcaaa taatggtatt gccgccgtga aagaaattag atccgtcaga 2100
aaatgggcat acgagacgtt cggcgatgac agaaccgtcc aattcgtcgc catggccacc 2160
ccagaagatc tggaggccaa cgcagaatat atccgtatgg ccgatcaata cattgaagtg 2220
ccaggtggta ctaataataa caactacgct aacgtagact tgatcgtaga catcgccgaa 2280
agagcagacg tagacgccgt atgggctggc tggggtcacg cctccgagaa tccactattg 2340
cctgaaaaat tgtcccagtc taagaggaaa gtcatcttta ttgggcctcc aggtaacgcc 2400
atgaggtctt taggtgataa aatctcctct accattgtcg ctcaaagtgc taaagtccca 2460
tgtattccat ggtctggtac cggtgttgac accgttcacg tggacgagaa aaccggtctg 2520
gtctctgtcg acgatgacat ctatcaaaag ggttgttgta cctctcctga agatggttta 2580
caaaaggcca agcgtattgg ttttcctgtc atgattaagg catccgaagg tggtggtggt 2640
aaaggtatca gacaagttga acgtgaagaa gatttcatcg ctttatacca ccaggcagcc 2700
aacgaaattc caggctcccc cattttcatc atgaagttgg ccggtagagc gcgtcacttg 2760
gaagttcaac tgctagcaga tcagtacggt acaaatattt ccttgttcgg tagagactgt 2820
tccgttcaga gacgtcatca aaaaattatc gaagaagcac cagttacaat tgccaaggct 2880
gaaacatttc acgagatgga aaaggctgcc gtcagactgg ggaaactagt cggttatgtc 2940
tctgccggta ccgtggagta tctatattct catgatgatg gaaaattcta ctttttagaa 3000
ttgaacccaa gattacaagt cgagcatcca acaacggaaa tggtctccgg tgttaactta 3060
cctgcagctc aattacaaat cgctatgggt atccctatgc atagaataag tgacattaga 3120
actttatatg gtatgaatcc tcattctgcc tcagaaatcg atttcgaatt caaaactcaa 3180
gatgccacca agaaacaaag aagacctatt ccaaagggtc attgtaccgc ttgtcgtatc 3240
acatcagaag atccaaacga tggattcaag ccatcgggtg gtactttgca tgaactaaac 3300
ttccgttctt cctctaatgt ttggggttac ttctccgtgg gtaacaatgg taatattcac 3360
tccttttcgg actctcagtt cggccatatt tttgcttttg gtgaaaatag acaagcttcc 3420
aggaaacaca tggttgttgc cctgaaggaa ttgtccatta ggggtgattt cagaactact 3480
gtggaatact tgatcaaact tttggaaact gaagatttcg aggataacac tattaccacc 3540
ggttggttgg acgatttgat tactcataaa atgaccgctg aaaagcctga tccaactctt 3600
gccgtcattt gcggtgccgc tacaaaggct ttcttagcat ctgaagaagc ccgccacaag 3660
tatatcgaat ccttacaaaa gggacaagtt ctatctaaag acctactgca aactatgttc 3720
cctgtagatt ttatccatga gggtaaaaga tacaagttca ccgtagctaa atccggtaat 3780
gaccgttaca cattatttat caatggttct aaatgtgata tcatactgcg tcaactatct 3840
gatggtggtc ttttgattgc cataggcggt aaatcgcata ccatctattg gaaagaagaa 3900
gttgctgcta caagattatc cgttgactct atgactactt tgttggaagt tgaaaacgat 3960
ccaacccagt tgcgtactcc atcccctggt aaattggtta aattcttggt ggaaaatggt 4020
gaacacatta tcaagggcca accatatgca gaaattgaag ttatgaaaat gcaaatgcct 4080
ttggtttctc aagaaaatgg tatcgtccag ttattaaagc aacctggttc taccattgtt 4140
gcaggtgata tcatggctat tatgactctt gacgatccat ccaaggtcaa gcacgctcta 4200
ccatttgaag gtatgctgcc agattttggt tctccagtta tcgaaggaac caaacctgcc 4260
tataaattca agtcattagt gtctactttg gaaaacattt tgaagggtta tgacaaccaa 4320
gttattatga acgcttcctt gcaacaattg atagaggttt tgagaaatcc aaaactgcct 4380
tactcagaat ggaaactaca catctctgct ttacattcaa gattgcctgc taagctagat 4440
gaacaaatgg aagagttagt tgcacgttct ttgagacgtg gtgctgtttt cccagctaga 4500
caattaagta aattgattga tatggccgtg aagaatcctg aatacaaccc cgacaaattg 4560
ctgggcgccg tcgtggaacc attggcggat attgctcata agtactctaa cgggttagaa 4620
gcccatgaac attctatatt tgtccatttc ttggaagaat attacgaagt tgaaaagtta 4680
ttcaatggtc caaatgttcg tgaggaaaat atcattctga aattgcgtga tgaaaaccct 4740
aaagatctag ataaagttgc gctaactgtt ttgtctcatt cgaaagtttc agcgaagaat 4800
aacctgatcc tagctatctt gaaacattat caaccattgt gcaagttatc ttctaaagtt 4860
tctgccattt tctctactcc tctacaacat attgttgaac tagaatctaa ggctaccgct 4920
aaggtcgctc tacaagcaag agaaattttg attcaaggcg ctttaccttc ggtcaaggaa 4980
agaactgaac aaattgaaca tatcttaaaa tcctctgttg tgaaggttgc ctatggctca 5040
tccaatccaa agcgctctga accagatttg aatatcttga aggacttgat cgattctaat 5100
tacgttgtgt tcgatgtttt acttcaattc ctaacccatc aagacccagt tgtgactgct 5160
gcagctgctc aagtctatat tcgtcgtgct tatcgtgctt acaccatagg agatattaga 5220
gttcacgaag gtgtcacagt tccaattgtt gaatggaaat tccaactacc ttcagctgcg 5280
ttctccacct ttccaactgt taaatctaaa atgggtatga acagggctgt ttctgtttca 5340
gatttgtcat atgttgcaaa cagtcagtca tctccgttaa gagaaggtat tttgatggct 5400
gtggatcatt tagatgatgt tgatgaaatt ttgtcacaaa gtttggaagt tattcctcgt 5460
caccaatctt cttctaacgg acctgctcct gatcgttctg gtagctccgc atcgttgagt 5520
aatgttgcta atgtttgtgt tgcttctaca gaaggtttcg aatctgaaga ggaaattttg 5580
gtaaggttga gagaaatttt ggatttgaat aagcaggaat taatcaatgc ttctatccgt 5640
cgtatcacat ttatgttcgg ttttaaagat gggtcttatc caaagtatta tacttttaac 5700
ggtccaaatt ataacgaaaa tgaaacaatt cgtcacattg agccggcttt ggccttccaa 5760
ctggaattag gaagattgtc caacttcaac attaaaccaa ttttcactga taatagaaac 5820
atccatgtct acgaagctgt tagtaagact tctccattgg ataagagatt ctttacaaga 5880
ggtattatta gaacgggtca tatccgtgat gacatttcta ttcaagaata tctgacttct 5940
gaagctaaca gattgatgag tgatatattg gataatttag aagtcaccga cacttcaaat 6000
tctgatttga atcatatctt catcaacttc attgcggtgt ttgatatctc tccagaagat 6060
gtcgaagccg ccttcggtgg tttcttagaa agatttggta agagattgtt gagattgcgt 6120
gtttcttctg ccgaaattag aatcatcatc aaagatcctc aaacaggtgc cccagtacca 6180
ttgcgtgcct tgatcaataa cgtttctggt tatgttatca aaacagaaat gtacaccgaa 6240
gtcaagaacg caaaaggtga atgggtattt aagtctttgg gtaaacctgg atccatgcat 6300
ttaagaccta ttgctactcc ttaccctgtt aaggaatggt tgcaaccaaa acgttataag 6360
gcacacttga tgggtaccac atatgtctat gacttcccag aattattccg ccaagcatcg 6420
tcatcccaat ggaaaaattt ctctgcagat gttaagttaa cagatgattt ctttatttcc 6480
aacgagttga ttgaagatga aaacggcgaa ttaactgagg tggaaagaga acctggtgcc 6540
aacgctattg gtatggttgc ctttaagatt actgtaaaga ctcctgaata tccaagaggc 6600
cgtcaatttg ttgttgttgc taacgatatc acattcaaga tcggttcctt tggtccacaa 6660
gaagacgaat tcttcaataa ggttactgaa tatgctagaa agcgtggtat cccaagaatt 6720
tacttggctg caaactcagg tgccagaatt ggtatggctg aagagattgt tccactattt 6780
caagttgcat ggaatgatgc tgccaatccg gacaagggct tccaatactt atacttaaca 6840
agtgaaggta tggaaacttt aaagaaattt gacaaagaaa attctgttct cactgaacgt 6900
actgttataa acggtgaaga aagatttgtc atcaagacaa ttattggttc tgaagatggg 6960
ttaggtgtcg aatgtctacg tggatctggt ttaattgctg gtgcaacgtc aagggcttac 7020
cacgatatct tcactatcac cttagtcact tgtagatccg tcggtatcgg tgcttatttg 7080
gttcgtttgg gtcaaagagc tattcaggtc gaaggccagc caattatttt aactggtgct 7140
cctgcaatca acaaaatgct gggtagagaa gtttatactt ctaacttaca attgggtggt 7200
actcaaatca tgtataacaa cggtgtttca catttgactg ctgttgacga tttagctggt 7260
gtagagaaga ttgttgaatg gatgtcttat gttccagcca agcgtaatat gccagttcct 7320
atcttggaaa ctaaagacac atgggataga ccagttgatt tcactccaac taatgatgaa 7380
acttacgatg taagatggat gattgaaggt cgtgagactg aaagtggatt tgaatatggt 7440
ttgtttgata aagggtcttt ctttgaaact ttgtcaggat gggccaaagg tgttgtcgtt 7500
ggtagagccc gtcttggtgg tattccactg ggtgttattg gtgttgaaac aagaactgtc 7560
gagaacttga ttcctgctga tccagctaat ccaaatagtg ctgaaacatt aattcaagaa 7620
cctggtcaag tttggcatcc aaactccgcc ttcaagactg ctcaagctat caatgacttt 7680
aacaacggtg aacaattgcc aatgatgatt ttggccaact ggagaggttt ctctggtggt 7740
caacgtgata tgttcaacga agtcttgaag tatggttcgt ttattgttga cgcattggtg 7800
gattacaaac aaccaattat tatctatatc ccacctaccg gtgaactaag aggtggttca 7860
tgggttgttg tcgatccaac tatcaacgct gaccaaatgg aaatgtatgc cgacgtcaac 7920
gctagagctg gtgttttgga accacaaggt atggttggta tcaagttccg tagagaaaaa 7980
ttgctggaca ccatgaacag attggatgac aagtacagag aattgagatc tcaattatcc 8040
aacaagagtt tggctccaga agtacatcag caaatatcca agcaattagc tgatcgtgag 8100
agagaactat tgccaattta cggacaaatc agtcttcaat ttgctgattt gcacgatagg 8160
tcttcacgta tggtggccaa gggtgttatt tctaaggaac tggaatggac cgaggcacgt 8220
cgtttcttct tctggagatt gagaagaaga ttgaacgaag aatatttgat taaaaggttg 8280
agccatcagg taggcgaagc atcaagatta gaaaagatcg caagaattag atcgtggtac 8340
cctgcttcag tggaccatga agatgatagg caagtcgcaa catggattga agaaaactac 8400
aaaactttgg acgataaact aaagggtttg aaattagagt cattcgctca agacttagct 8460
aaaaagatca gaagcgacca tgacaatgct attgatggat tatctgaagt tatcaagatg 8520
ttatctaccg atgataaaga aaaattgttg aagactttga aataaaacta acgctaatcg 8580
ataaaacatt agatttcaaa ctagataagg accatgtata agaactatat acttccaata 8640
taatatagta taagctttaa gatagtatct ctcgatctac cgttccacgt gactagtcca 8700
aggatttttt ttaaaggaag acatagtttt ttactttccc ccctgccctt cataaacact 8760
acgtttcatt ttctaagagc atcaatttgc aaacacctga actacttgca caatacatag 8820
tttcttctcc tacagataca gttaataatt aataaataac attacacata aacatgttta 8880
agtttcattt cagcaagaca agattgacat tattaagcgt gatctaaact atgaatttct 8940
cccgggtatg aaactacatt ctactattga ttttaataga tgagcgatta aacgggatat 9000
ttgaatttat gactgtttaa acatcgtcga cctgcaggca tgcaagcttg gcgtaatcat 9060
ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 9120
ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 9180
cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 9240
tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 9300
ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 9360
taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 9420
agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 9480
cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 9540
tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 9600
tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 9660
gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 9720
acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 9780
acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 9840
cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 9900
gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 9960
gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 10020
agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 10080
ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 10140
ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 10200
atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 10260
tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 10320
gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 10380
ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 10440
caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 10500
cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 10560
cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 10620
cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 10680
agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 10740
tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 10800
agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 10860
atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 10920
ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 10980
cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 11040
caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 11100
attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 11160
agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct 11220
aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc 11280
gtc 11283
<210> 13
<211> 17748
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 13
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420
cctctagaga ttgcggccgc gtcaaagcac taatacattg gggctttaag agtgtttata 480
aaggtctaac ctgtaaaaat tatttaaaca acttgaacag gccttaaagt tttcctcatt 540
ccgctcatca tcactaatat tgctctccgt ttttgaatac acacttgaca ctaataagta 600
tcacagaaaa aaagaaaata taataaatta gtattgcgat tacacggtcc aatggataaa 660
cattttttat caacactatg atatataaat ataatagttt ttcgtatata tattcctttt 720
tttggtcaat ttttgaaatt ttcgtagaaa agggagagac aaaacacatt atatcaatga 780
aaacgtacaa aaagtagata aagtcagtgc ttaaacacgt cttttcctta aaaatacttt 840
attattttta ttttattgag agggtggttt aaaaatagaa atagagagag aggtacatac 900
ataaacatac gcgcacaaaa gcagagacta tttcttagta gaaacggcga ccgcgacagc 960
ttggaggtca tcgtgagaaa tagatacctt cacatcggta acaccagctt cttcggcagc 1020
ctttttggcg ttaccgtgca gttcaacggc tggagcgttt ttgttaacgc gtacgatttc 1080
gatgtctttc aatgcagcac caccgcctaa ggacttgacg cctaaggact tgaaaacagc 1140
ctctttggcg gaccatgtcc cagcgaaaga gctttgcaca ctaggctgcg cgctgcagta 1200
ctctatttct tgcggggtga aattgcgctc gataaaagta tcattttcaa cgttgatgct 1260
agtgattaat tcaacatcta caccgacgcc accgttagag actttctcct tggtcatgtt 1320
ttcaatcatc ttggcagttt caatggtgtt agcattgatg taactgtcct tgctttggat 1380
gtttttagag ttgaaagtca aggagcctga tttcttatcc ttagatacac gggctaatgg 1440
gtccaagtaa acatcctctt ccaattcatc agtgtatgga gcatgctctt tacttacgaa 1500
caacttgttg tagatcatac cattatggaa gaatttgtag gcacttttct ctctggcact 1560
aaccttggcg acatactcgt tgtatctgtc ttcagtgata gcaccgtata agtagtctgg 1620
atgaaccaca atagcttgac cacccttttg accaaaaccg aaagaagtga tggacacggc 1680
tctgacaccg tcggtcttta aagtcttgga tgggtacaag acgtattcaa attgctccaa 1740
gatcttatcc acgttatcag cgttacggtt acctggaata ataccactgt ttagaatttg 1800
caaagcacca ttcatcatcc atgcaccagc agcacccttt ggatgaccag tcaagaactt 1860
ttggaaaaca ccaatgacgg gattaccttc agatctaccc aaatgcttca tcatttcatt 1920
aattgtggca gattcgttct tgtcattagc ctttgtggat gtaccgtgga atgaagcgac 1980
acccaagtca tcaatagtta aaccgtaagt agccagtgct cctctcaatg gagcaatacg 2040
tgggtccctc ttgtagaagt cgttacccca ttgttgttgt gcagctctca attgactttc 2100
agcttcgttg tggatttctc tggtacgttc aagtaagaac tcgttttggt cttcgcttgg 2160
aatttcttcg gcctccaact tcaaagcttc caattcgttt tctacccaat ctttaatctg 2220
agcttcacga gtaaccaatt ggcgctttct gtacttcatg ttcaagtttg gtgaagcata 2280
cttaacactg gagtggtgtt cacgagcagt ggttaaaata cccttacctg gagctggcac 2340
agatctacca atcttatcgg tggcggtagc agccatggca acaataccgt aaattggcac 2400
acccatcttc aaagctaaat cagcttgcat gatgatttga ataccagcac cttgagcttc 2460
cataaaaccg ttacgggtag tggtggcagg tctggacatt tccgctgggg tacgaccatg 2520
ttcaaattct tccaaagtgt tggaagtggc cttcatgtta ccgaactcaa aggagccttc 2580
ttcttggaaa tcatcgtaac caccgacaat acagattcta gccttaccag acaagatggt 2640
ttctacacca atgtcaacag attccacgga tgtggcacag gcaccaacag gtgtcttgat 2700
tggaccagat gaggaaatca acaacatatt aacccaagcg gacatggtgt tgataaatga 2760
ttcttgtaaa atatcatttt ggacaggctc atccttgaaa cggtccttaa acataccacg 2820
taaggcagaa acaccaccca taccagaacc agaacagtta ccaacctcag aaacatgtac 2880
gtatttgtac atttcgtatg ggtcggtgat accagatgca ataaatgctt ccacaacaga 2940
gaccaaaacg aataatgtga ttgggtcaac ctgagaaatg atatcatcag agataccata 3000
agtcttagca ttccaaccag ttggaatttg acctgcaacc aaacggtcaa atctcaaagc 3060
ctttggaatg tataaagtgg caccctttag taacttaaca gagtactctc ctgtttctgg 3120
gatttcgaag atatccactt tgtcaccatg ttggtgttta aattgttcgg cagtttcctt 3180
cgaagcctca aatggttcca agtcttcttc gacaatgact tcttgaatca tttccttctt 3240
ttctgggttg taaccattga ataactctgg ttcgatcaat ctgataccac tgtgttctag 3300
gattgatgtt tcatacttgg ccttaacgtc cttgtcatca actggttctt ttgttttgga 3360
atcaacccaa ccagtgtatg gacgaccctt caaattaccg ttatggtatg aaatgaagcc 3420
cataatccag gccatttcaa cgcaaccttc caacgaaaat tcaccaaaag cttccatttc 3480
ccatcttgtt ctggccgaac cccatgggcc gacttcagca aaaccggtaa ctacaataac 3540
tctttccaaa tccaacaaac cttcaagctc agcgggagca atttgtttaa cctgtttgta 3600
tggtttcaat tctgggaagt ccagttgaat gttagctctt ggttgaattt cgacttgagc 3660
atatgcagca tcagcgctat tgccattgac aaccttatgc tccaaagcag tttcgatgga 3720
aactgccttt ctaacttcag aagtttcaac caactcttta cgcaatttag cagtgaattc 3780
cttcaattca ggaacaaatt gcaaaccacc attcaagtca gccataacag gtgatttttg 3840
gcacaattct acgacttctg gagtcaatag acccaataag ttgaaagcca tttccttttg 3900
agagaaagta cgaacaccca tcttttcaat gccttcagca atgatgttat tagcgctcat 3960
taaaccagta cctctagtcc aaccaataat agcaccgcaa acggttaatt gattggccca 4020
ggattcagag tgccatctgt tgaacaaagt ttccaaagac aacttggatt ctgaatacat 4080
accatcacca ccgaaagtac catggtttgg agacattggt agaatgactt gagctggtct 4140
tgtttcaata cctcttgcag atttttgctt cttgacacaa cccatcattc ttaagatatt 4200
ggtcaacatg attctatgag caaattcaga cttagaatca atatgttcta attcaatacc 4260
ttgttctgga atggccgcga atggaataat agcatctaga tcccaaccta aaccaccatt 4320
cttttcagtg tcgtagataa attcaatcaa agcttcaacg tcttgcttag aaccttggtt 4380
gaatggaaca acaatcaaag tagaaccctt agcaccatat ttggcgtaaa tggattggta 4440
gtagtctgta acttgcttag agaaacgaga ggtggtaaca acaaccttag caccaccttg 4500
taacaaacct tgcaagactt cagcaccaat agaaccctta ccagcaccag tgatcaagac 4560
gtatttgtcc ttgaaggtga caccgttgaa ggcagccttt tctaaaccat ctaagaaaag 4620
agaagacaat tggcggtcat atttccaatc tccggcagga gtcttctttc tcaaatgtaa 4680
gaacggaata gtttctcttg gaatagttga agataccgtc ttagaaatgg tagatttgtt 4740
tggcaaagaa gcaacttctt tggtggaatc tttgtccaaa gcatcctcaa catccatacc 4800
ggccaattgg gttgtttgag aaggatcgat ttctttggag ctttccaaga acttcatcaa 4860
atcactatat agtttttcaa attcaaccct ggtggagctg gaaatatctt gtttatcagc 4920
ttgagcactg attgccttgt aaacacgagt caaatcctct tcaatagtag gttgagattc 4980
tttggtgatt ggaccaccaa gggccatttc ttgtacgtat tgagataatt tcctaacctt 5040
ttctcttggc tcttctgagt atgtaatgtt accgttcttg tcaatagcag tttttggacc 5100
ggtaggctta gcaacatctt tgtaaactgg atcaacatcc aaaacttgtt tacagttttc 5160
aatcaactgc tcaccaagag ttttaaccaa ttgatagttt tcaccttttg tttcatcagt 5220
gttagagata tggtattcca tgaatttaat caaagcatcg ttagatctgt tcatgatatt 5280
gatagcttca ctaacaacct ctctatcaac gtttttcaag acaccatgaa ttatctcaaa 5340
gtataatgat aataaagatt gtttagccca gttccaggaa gaatcgaagg ttctggcctt 5400
ttttctagag aaagaagtag caacaccgtt aacaaagaat tcacctaatt cggcattcaa 5460
gtaatccaac tgagcttgaa gttcagcaac agtgtccttt tctttcaaga actttctttc 5520
accgttatcc aagtccattt ttagataacg agccaatact tgcagttgtt gacgcgccaa 5580
aaccttgtgg tctttggtta tttcttccag agcgccagca tcgatcatag ctgcaccggc 5640
agcagcacct gcaccggcag caccactagc gctagcagct gatgataagt caacaccaac 5700
aatggaagcg tatttttgag ccatggagtc caagaaagcc ttggcatcag cttcagaacc 5760
tagacgagca gcaggctcgt tagataaagc taccaaaagg acaccatctt gtctaccaga 5820
tggtagtccc cagcgagttt gtaagtattt tctagcgaca gtaatagtaa acccaccagg 5880
catcttagat gagattaatc ttgataataa cgaggaagat tgcttaccca atgctccaga 5940
gaaggtatct tggaaagttt ctgccaattc ttctaatgga gtttcttctg gtttttcagg 6000
agtagtacca aattctttac ctaaatcacc caaaatttca ttttggactg tagatttacc 6060
accgaccaag tctttgattg tcttggacat tggaatggaa tctaacgact tcttcaactt 6120
gtgagcaacc aaaacgtgca acaataggga agccttgaca ggttcatcgg caatctcagc 6180
agctgctgca gctggggctg ctgccgcgac gggagctggg gctgctgctg caggagcagg 6240
agcactagca gctggagttg gagcaggagc ttcttcctta gcgggctctt cctttgcagc 6300
tagttcggat ggatctgggg tataataaat ctctttggca tccttcgaat agcataagat 6360
ttctctatgt aaagacagag cagcatcgta agattcgtat ttattcttca aggttctttg 6420
agccatccca gccaaagttg gagaaggacc gatttcaaca accctttcag tgttaaaatc 6480
cttcaaaaaa acatcttgag tttcaatcca tctcacagga gaggcaaatt gataagctaa 6540
caattcagtt agcaaaatat gagctaattc ttgctcaact tccggcttca tctcgaggat 6600
tttacgtata tcaactagtt gacgattatg atatctttat agattttaag gtggggaaag 6660
aacatgagac cccagatgga attgattatg gggacattgt tgcctttata tataatttca 6720
atatactaat tcaaatgatt aaaaacgtga gggggacacg caacttcggg tgttaagaaa 6780
tattttgcta cattagataa tggtggagtt tcctggcttg tcggataaaa gccatcaaat 6840
gtcgcagcag ctcatgttta cgtttgctgt cttctgccca cgtcatatga gtggtattct 6900
tctatcagca cttgatgaat attctttttc tcatatatct gaaagacaaa agatcggcac 6960
ggcaatgccc tgcagcattt cttcctagtt tttccgaatt tccattacgt attggatctt 7020
gtgcgcatat ttgtcagtcc ttcacggaaa aaaaaaaaga gcactgggtc acttcggaaa 7080
aacttttgac tcaatgcaac agtgtcataa tcctttgcgc tgtctctttg aagaaaaatc 7140
aggagtgcaa gatatcgatt aattccttgg aagttatgat ggttagtctt agtttaactc 7200
tcttgaagaa gggttttttc agttggtcaa cactctttag aggtaaaaaa aaaaaaaaaa 7260
aaaaaaaaaa gagaattctt catgtaattt accatgattc tacgtttttg caagcaaaaa 7320
tgaagataat ccgagcgcat gcgcaagtag tccctgccat gccgcttcgg aaaaactttc 7380
gaaaccaata ctccttacat ccgccttgaa tttaggacct acaattgttg tctcttctgc 7440
ttgaaccaat aagccctaga aaccttacac cctaatttgc acaagaaaac tacgaaaaaa 7500
ttttttcctc ttttttccgc agctccaatc tcgccctgct atttttttgg ttcggagaaa 7560
aatagtcggt cgatatgaat tgaagtttca ttgtggccac tcgtttagga atggaataac 7620
ctccgcgaat ggaaaaaaag aactcggaat atcaaaaatt ccggctcaaa ggaaaaattt 7680
gcttcaggag gggggaatta tattccagat gagacctgca tgcaggtctc atctggaata 7740
taattccccc ctcctgaagc aaatttttcc tttgagccgg aatttttgat attccgagtt 7800
ctttttttcc attcgcggag gttattccat tcctaaacga gtggccacaa tgaaacttca 7860
attcatatcg accgactatt tttctccgaa ccaaaaaaat agcagggcga gattggagct 7920
gcggaaaaaa gaggaaaaaa ttttttcgta gttttcttgt gcaaattagg gtgtaaggtt 7980
tctagggctt attggttcaa gcagaagaga caacaattgt aggtcctaaa ttcaaggcgg 8040
atgtaaggag tattggtttc gaaagttttt ccgaagcggc atggcaggga ctacttgcgc 8100
atgcgctcgg attatcttca tttttgcttg caaaaacgta gaatcatggt aaattacatg 8160
aagaattctc tttttttttt tttttttttt ttttttacct ctaaagagtg ttgaccaact 8220
gaaaaaaccc ttcttcaaga gagttaaact aagactaacc atcataactt ccaaggaatt 8280
aatcgatatc ttgcactcct gatttttctt caaagagaca gcgcaaagga ttatgacact 8340
gttgcattga gtcaaaagtt tttccgaagt gacccagtgc tctttttttt tttccgtgaa 8400
ggactgacaa atatgcgcac aagatccaat acgtaatgga aattcggaaa aactaggaag 8460
aaatgctgca gggcattgcc gtgccgatct tttgtctttc agatatatga gaaaaagaat 8520
attcatcaag tgctgataga agaataccac tcatatgacg tgggcagaag acagcaaacg 8580
taaacatgag ctgctgcgac atttgatggc ttttatccga caagccagga aactccacca 8640
ttatctaatg tagcaaaata tttcttaaca cccgaagttg cgtgtccccc tcacgttttt 8700
aatcatttga attagtatat tgaaattata tataaaggca acaatgtccc cataatcaat 8760
tccatctggg gtctcatgtt ctttccccac cttaaaatct ataaagatat cataatcgtc 8820
aactagttga tatacgtaaa atccctagga tggacgctta ctccacaaga ccattaaccc 8880
tatctcacgg ttctttagag cacgtgcttc tggtaccaac cgcttcattt ttcattgctt 8940
cgcaattaca agaacaattt aataaaattt tgcccgaacc cactgaaggg tttgctgcag 9000
atgacgagcc taccacacct gctgaactag tggggaaatt ccttggctac gtatcttctc 9060
tagtcgaacc ttccaaggtc ggtcaattcg atcaggtctt gaacctttgc ttaacagaat 9120
ttgaaaactg ttatttagaa ggcaatgaca ttcacgcctt ggctgctaaa ctattacagg 9180
aaaacgacac aactttagtg aagactaaag aactaattaa aaattatatt accgccagaa 9240
taatggctaa gagaccattt gacaaaaaat ccaactctgc tctttttagg gccgtcggcg 9300
agggtaacgc acaattggta gccattttcg gtggtcaagg taacaccgac gactactttg 9360
aagaattgcg tgatctatat caaacttatc atgtcttagt gggagattta atcaagttct 9420
ccgctgaaac tttaagtgaa ctgattagaa ctactttaga tgctgaaaaa gtctttactc 9480
aaggtttaaa catattggaa tggttggaga acccttcaaa taccccagac aaggactatt 9540
tactttccat tccaatttca tgccccttaa ttggtgtcat tcaattggct cactacgtag 9600
ttactgccaa gcttttgggt ttcactccag gtgagttaag atcttactta aaaggtgcta 9660
caggtcactc tcaaggtttg gttactgctg tcgccatagc tgagacggat tcctgggaat 9720
ccttcttcgt ctccgtaaga aaagcaatta ctgtattatt cttcatcggt gttcgttgtt 9780
acgaagcata cccaaacact tccctaccac catccatctt ggaagattcc ttggaaaaca 9840
atgaaggtgt tccatctcca atgttgtcca tttccaatct aactcaagaa caagttcaag 9900
actatgtaaa taagactaac tctcatttgc cagctggtaa acaagttgaa atttctctag 9960
tcaatggtgc gaagaatcta gtcgtatcgg gcccaccaca atcattatat ggtttaaact 10020
tgactttaag aaaggccaag gccccatctg gactggatca atcaagaatc ccattcagcg 10080
aaagaaaatt gaagttctcc aataggttct tacctgttgc atcaccattc cattcccatc 10140
tattggttcc agcttcagat ttgattaaca aagacttagt caaaaacaat gtcagcttta 10200
acgctaaaga tattcaaatc cccgtttacg acacttttga tggttcagat ctaagagtcc 10260
tttcaggttc catttccgag agaatcgtcg actgcatcat tagattacct gtcaaatggg 10320
aaactactac acaattcaaa gccacccaca tattagactt tggtccaggt ggagcttccg 10380
gtttaggtgt tttaacccat cgtaataaag atggtactgg tgttcgtgtt atcgttgccg 10440
gtactctcga cattaaccca gatgatgatt acggattcaa gcaagaaatc tttgatgtta 10500
ctagtaatgg tttgaagaaa aatccaaact ggttggaaga ataccatcca aaattaatta 10560
agaacaaatc aggcaaaatt tttgtcgaaa caaaattttc taaattaatc ggtagaccac 10620
ctttattggt tcctggtatg acaccatgta ctgtttctcc agatttcgta gctgctacca 10680
caaatgctgg ttataccatt gagttggccg gtggtggtta cttttccgca gcaggtatga 10740
ccgccgctat tgattctgtg gtttctcaga tagaaaaggg tagtaccttc ggtatcaact 10800
tgatctacgt caatccattt atgttacaat ggggtattcc attaatcaag gaactaagaa 10860
gcaaaggtta tccaattcaa ttcttgacca ttggtgctgg tgtcccatca ttggaagttg 10920
ctagtgaata catagagaca ttaggtttga agtacttggg tttgaaacca ggttccattg 10980
atgctatttc gcaagttata aacattgcta aagcacatcc aaacttccca atagctttac 11040
aatggaccgg tggtagaggt ggtggtcatc attctttcga agatgcccac actccaatgt 11100
tacaaatgta ctccaagatt agaagacatc caaacattat gttgatattc ggttctggtt 11160
tcggttctgc tgatgacact tacccatact taaccggtga atggtccaca aaattcgatt 11220
atccaccaat gccattcgat ggtttcctat ttggttcgag ggtcatgatt gctaaggaag 11280
ttaaaacttc tcctgatgct aagaagtgta ttgctgcttg tactggtgtt cctgatgata 11340
aatgggaaca aacctacaag aagccaactg gtggtattgt cactgttcgc tctgaaatgg 11400
gtgaaccaat tcacaaaatt gccactcgtg gtgttatgct atggaaggaa ttcgacgaaa 11460
ccatcttcaa cttaccaaag aataagttgg taccaacttt ggaagcaaag agagattaca 11520
ttatctcaag attgaacgcc gatttccaaa aaccatggtt tgctaccgtc aacggtcaag 11580
cccgtgacct agccacaatg acatacgaag aagttgcaaa gagattggtg gaattaatgt 11640
tcatcagatc taccaactct tggtttgatg tcacatggag aacctttact ggtgatttcc 11700
tacgtcgtgt cgaagaacgt ttcactaaaa gtaagacatt gtctttaatc caatcctatt 11760
ctctactaga caagcctgat gaagctattg aaaaagtatt taatgcttat cctgccgcta 11820
gggaacagtt cttgaatgcg caagatattg atcacttttt gagcatgtgt caaaatccaa 11880
tgcaaaaacc agtgcctttt gttccagttt tggatcgtag attcgagatt tttttcaaaa 11940
aagattcgtt atggcaatct gagcacttgg aagccgtcgt cgaccaagac gttcaaagaa 12000
catgtatcct acatggacct gttgcagcac aattcactaa agtcatcgat gaaccaatta 12060
agagcattat ggatggtatt cacgatggtc acatcaaaaa gttactacat caatattacg 12120
gtgacgatga gtcaaagatt ccagcagttg agtactttgg tggtgaaagc cctgtagacg 12180
tacaaagtca agttgattct tcctctgtat ctgaagactc agctgttttt aaggcaacat 12240
cctctactga tgaagaaagc tggtttaagg ctttggcggg atccgaaatt aactggagac 12300
atgcaagttt cttatgttcc tttatcactc aagataaaat gtttgtttct aacccaatta 12360
gaaaagtttt caagccaagc caaggaatgg ttgttgagat ttccaacggc aatacttctt 12420
caaagactgt tgtcactctt tcagaacctg ttcaaggtga attgaaacca actgttattt 12480
tgaagttgtt gaaggagaac ataatccaaa tggaaatgat tgagaacaga actatggatg 12540
gtaagcccgt cagcttgcca ttgttgtaca acttcaaccc agataatggt tttgctccaa 12600
tctctgaagt tatggaggac agaaaccaaa gaattaagga aatgtactgg aaattatgga 12660
ttgatgagcc tttcaatttg gactttgacc caagagatgt cattaagggc aaagatttcg 12720
agatcaccgc taaagaagtt tatgacttta cacacgctgt tggaaacaat tgtgaagact 12780
tcgtttctag acctgataga acgatgttgg ccccaatgga ctttgctatt gttgtcggat 12840
ggagagccat catcaaggcc attttcccta atacggtcga tggtgactta ttgaagttgg 12900
ttcatttgtc taacggctac aagatgattc ctggcgctaa gccactgcaa gttggtgatg 12960
ttgtttcaac tactgctgtt attgaatctg tcgtcaacca acctacagga aagattgtcg 13020
atgtggtagg tacattatcg agaaatggca agcctgtcat ggaagtcacc tcctcattct 13080
tctacagagg caactatact gactttgaaa acactttcca aaagactgtt gaacctgttt 13140
atcaaatgca catcaaaact tctaaagata tagctgtctt gcgctctaag gagtggttcc 13200
aattggacga tgaagacttc gatctgttaa acaaaacttt gactttcgaa actgaaactg 13260
aagttacttt caagaatgct aacatcttct cttcagtgaa atgttttggc ccaattaaag 13320
ttgaattgcc aaccaaagaa accgtggaga tcggtattgt cgattacgaa gccggtgcct 13380
ctcacggtaa ccctgttgtt gatttcttga agagaaacgg ttccacattg gaacaaaagg 13440
tcaatctaga aaatcctatt ccaattgcag tacttgattc gtacactcca agtaccaacg 13500
aaccatacgc tagagtttct ggtgatttga atccaattca cgtttcacgt cattttgcct 13560
cttacgcaaa cttgccaggt actatcacgc acggtatgtt ttcttctgct tccgtccgtg 13620
ctttgattga aaactgggct gctgacagtg tttcatccag ggtacgtggc tacacttgtc 13680
aatttgttga catggttttg cctaacactg ctttgaaaac atcgattcaa catgttggta 13740
tgatcaatgg tagaaaattg ataaagtttg aaactagaaa tgaagatgac gttgtagttt 13800
tgactggtga agccgaaatt gaacaacctg ttactacctt cgttttcact ggtcaaggtt 13860
cacaagaaca aggtatgggt atggacttat acaaaacttc taaagctgct caagatgttt 13920
ggaatagagc tgacaaccat ttcaaggaca cttatggttt ctctatctta gacattgtca 13980
ttaacaaccc agttaactta acaattcact tcggtggtga aaagggtaag aggatcagag 14040
aaaactattc tgctatgatc tttgagacta tcgtggatgg aaaattgaag actgaaaaaa 14100
ttttcaagga aattaatgag cacagtactt cttacacatt tagatctgaa aaaggtttat 14160
tgtctgctac tcaatttaca caaccagctt taactttgat ggaaaaagct gctttcgaag 14220
acttgaaatc taaaggtttg atcccagccg atgctacttt tgctggtcac tctttaggtg 14280
agtatgctgc tttggcctct ttggctgatg ttatgtctat cgaatcttta gttgaagttg 14340
tgttctacag aggtatgact atgcaagttg ctgttccaag agatgagttg ggcagatcca 14400
actatggtat gattgccatt aacccaggta gagtcgctgc atcattctct caagaagctt 14460
tgcaatatgt tgttgagaga gttggtaaga gaaccggctg gttggttgaa atcgtcaact 14520
acaacgttga aaaccaacaa tatgttgcag ctggtgatct aagagcttta gacaccgtta 14580
ccaatgttct aaacttcatc aaattacaaa aaattgatat tattgaacta caaaagtcct 14640
tatctttgga agaagttgaa ggtcatttgt ttgagatcat tgacgaagct tccaagaaat 14700
ctgctgtcaa gcctcgccca cttaaattgg agagaggttt tgcttgtatc ccattagttg 14760
gtatttctgt tcctttccat tccacctact tgatgaatgg tgttaaacca ttcaagagtt 14820
tcttgaagaa gaatatcata aaagaaaatg tgaaggttgc tagattggcc ggaaagtaca 14880
ttccaaactt gactgcaaaa ccattccagg ttactaagga atatttccag gacgtttatg 14940
atttgactgg ctccgaacct atcaaggaaa tcatcgacaa ctgggaaaag tatgaacaat 15000
cctaaattga attgaattga aatcgataga tcaatttttt tcttttctct ttccccatcc 15060
tttacgctaa aataatagtt tattttattt tttgaatatt ttttatttat atacgtatat 15120
atagactatt atttatcttt taatgattat taagattttt attaaaaaaa attacgctcc 15180
tcttttaatg cctttatgca gttttttttc ccattcgata tttctatgtt cgggttcagc 15240
gtattttaag tttaataact cgaaaattct gcgttcgttg tagaggtttc ctgttttcct 15300
tcgaaccctc tgttttgcga cttttgtttc aattcaacta gtgtcgccaa gttttaacaa 15360
aaagttacaa aatcctagtg agaggccatc ttatgtgcat aacggtactc tctatctatt 15420
tacatatcta atactattca cataactatg acgaatcaat gacatgacta catttaccag 15480
cggccgcatc gtcgacctgc aggcatgcaa gcttggcgta atcatggtca tagctgtttc 15540
ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 15600
gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 15660
ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 15720
ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 15780
cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 15840
cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 15900
accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 15960
acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 16020
cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 16080
acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 16140
atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 16200
agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 16260
acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 16320
gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 16380
gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 16440
gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 16500
gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 16560
acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 16620
tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 16680
ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 16740
catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 16800
ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 16860
caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 16920
ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 16980
tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 17040
cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 17100
aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 17160
tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 17220
gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 17280
cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 17340
aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 17400
tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 17460
tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 17520
gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 17580
atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 17640
taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 17700
tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc 17748
<210> 14
<211> 9921
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 14
tgatccaata tcaaaggaaa tgatagcatt gaaggatgag actaatccaa ttgaggagtg 60
gcagcatata gaacagctaa agggtagtgc tgaaggaagc atacgatacc ccgcatggaa 120
tgggataata tcacaggagg tactagacta cctttcatcc tacataaata gacgcatata 180
agtacgcatt taagcataaa cacgcactat gccgttcttc tcatgtatat atatatacag 240
gcaacacgca gatataggtg cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc 300
attttcggaa gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa gttcctattc 360
tctagaaagt ataggaactt cagagcgctt ttgaaaacca aaagcgctct gaagacgcac 420
tttcaaaaaa ccaaaaacgc accggactgt aacgagctac taaaatattg cgaataccgc 480
ttccacaaac attgctcaaa agtatctctt tgctatatat ctctgtgcta tatccctata 540
taacctaccc atccaccttt cgctccttga acttgcatct aaactcgacc tctacatttt 600
ttatgtttat ctctagtatt actctttaga caaaaaaatt gtagtaagaa ctattcatag 660
agtgaatcga aaacaatacg aaaatgtaaa catttcctat acgtagtata tagagacaaa 720
atagaagaaa ccgttcataa ttttctgacc aatgaagaat catcaacgct atcactttct 780
gttcacaaag tatgcgcaat ccacatcggt atagaatata atcggggatg cctttatctt 840
gaaaaaatgc acccgcagct tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt 900
tttttatgga agagaaaata gacaccaaag tagccttctt ctaaccttaa cggacctaca 960
gtgcaaaaag ttatcaagag actgcattat agagcgcaca aaggagaaaa aaagtaatct 1020
aagatgcttt gttagaaaaa tagcgctctc gggatgcatt tttgtagaac aaaaaagaag 1080
tatagattct ttgttggtaa aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc 1140
agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa 1200
tgaagcacag attcttcgtt ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa 1260
aatgcagctc agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttctac 1320
aaaatgaagc acagatgctt cgttcaggtg gcacttttcg gggaaatgtg cgcggaaccc 1380
ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct 1440
gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg 1500
cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg 1560
tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc 1620
tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca 1680
cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac 1740
tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa 1800
agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg 1860
ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt 1920
ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg 1980
aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc 2040
gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga 2100
tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta 2160
ttgctgataa atctggagcc ggtgagcgtg ggagccgcgg tatcattgca gcactggggc 2220
cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg 2280
atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt 2340
cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa 2400
ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt 2460
cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt 2520
ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt 2580
tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga 2640
taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag 2700
caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata 2760
agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg 2820
gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga 2880
gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca 2940
ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa 3000
acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt 3060
tgtgatgctc gtcagggggg cggagcctat ggaaagcggc cgcacaatct ttgaaaagat 3120
aatgtatgat tatgctttca ctcatattta tacagaaact tgatgttttc tttcgagtat 3180
atacaaggtg attacatgta cgtttgaagt acaactctag attttgtagt gccctcttgg 3240
gctagcggta aaggtgcgca ttttttcaca ccctacaatg ttctgttcaa aagattttgg 3300
tcaaacgctg tagaagtgaa agttggtgcg catgtttcgg cgttcgaaac ttctccgcag 3360
tgaaagataa atgatccaca tttttagagt catcccgttt tagagctaga aatagcaagt 3420
taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcaaacaagc 3480
gcaagtggtt tagtggtaaa atccaacgtt gccatcgttg ggcccccggt tcgattccgg 3540
gcttgcgcat ctgaggacag gaggcaaagg ttttagagct agaaatagca agttaaaata 3600
aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcaaaca agcgcaagtg 3660
gtttagtggt aaaatccaac gttgccatcg ttgggccccc ggttcgattc cgggcttgcg 3720
cagaatcctg aagaaactgc tggttttaga gctagaaata gcaagttaaa ataaggctag 3780
tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttctttt ttgtccacca 3840
ccaaaatgtc aatttttttg ccattttcta aatagttttc ttcactctac aagagatatc 3900
acgattgttt gctttcgata ttggatccat ctaaagtcat ttcaactatc taagatgtgg 3960
ttgtgattgg gccattttgt gaaagccagt acgccagcgt caatacactc ccgtcaatta 4020
gttgcaccat gtccacaaaa tcatatacca gtagagctga gactcatgca agtccggttg 4080
catcgaaact tttacgttta atggatgaaa agaagaccaa tttgtgtgct tctcttgacg 4140
ttcgttcgac tgatgagcta ttgaaacttg ttgaaacgtt gggtccatac atttgccttt 4200
tgaaaacaca cgttgatatc ttggatgatt tcagttatga gggtactgtc gttccattga 4260
aagcattggc agagaaatac aagttcttga tatttgagga cagaaaattc gccgatatcg 4320
gtaacacagt caaattacaa tatacatcgg gcgtttaccg tatcgcagaa tggtctgata 4380
tcaccaacgc ccacggggtt actggtgctg gtattgttgc tggcttgaaa caaggtgcgc 4440
aagaggtcac caaagaacca aggggattat tgatgcttgc tgaattgtct tccaagggtt 4500
ctctagcaca cggtgaatat actaagggta ccgttgatat tgcaaagagt gataaagatt 4560
tcgttattgg gttcattgct cagaacgata tgggaggaag agaagaaggg tttgattggc 4620
taatcatgac cccaggtgta ggtttagacg acaaaggcga tgcattgggt cagcagtaca 4680
gaaccgtcga cgaagttgta agtggtggat cagatatcat cattgttggc agaggacttt 4740
tcgccaaggg tagagatcct aaggttgaag gtgaaagata cagaaatgct ggatgggaag 4800
cgtaccaaaa gagaatcagc gctccccatt aattatacag gaaacttaat agaacaaatc 4860
acatatttaa tctaatagcc acctgcattg gcacggtgca acactacttc aacttcatct 4920
tacaaaaaga tcacgtgatc tgttgtattg aactgaaaat tttttgtttg cttctctctc 4980
tctctctttc attatgtgag atttaaaaac cagaaactac atcatcgaaa aagtctagag 5040
ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg 5100
gcaccgagtc ggtgcttttt ttattttttg tcactattgc atgccggtag aggtgtggtc 5160
aataagagcg acctcatgct atacctgaga aagcaacctg acctacagga aagagttact 5220
caagaataag aattttcgtt ttaaaaccta agagtcactt taaaatttgt atacacttat 5280
tttttttata acttatttaa taataaaaat cataaatcat aagaaattcg cctcgagtca 5340
gacctttctc ttcttttttg gagggtcacc tcctagctga ctcaaatcaa tgcgtgtttc 5400
ataaagacca gtgatggatt gatggataag agtggcatct aaaacttctt ttgtagacgt 5460
atatcgttta cgatcaattg ttgtatcaaa atatttaaaa gcagcgggag ctccaagatt 5520
cgtcaacgta aataaatgaa taatattttc tgcttgttca cgtattggtt tgtctctatg 5580
tttgttatat gcactaagaa ctttatctaa attggcatct gctaaaataa cacgcttaga 5640
aaattcactg atttgctcaa taatctcatc taaataatgc ttatgctgct ccacaaacaa 5700
ttgtttttgt tcgttatctt ctggactacc cttcaacttt tcataatgac tagctaaata 5760
taaaaaattc acatatttgc ttggcagagc cagctcattt cctttttgta attctccggc 5820
actagccagc atccgtttac gaccgttttc taactcaaaa agactatatt taggtagttt 5880
aatgattaag tcttttttaa cttccttata tcctttagct tctaaaaagt caatcggatt 5940
tttttcaaag gaacttcttt ccataattgt gatccctagt aactctttaa cggattttaa 6000
cttcttcgat ttcccttttt ccaccttagc aaccactagg actgaataag ctaccgttgg 6060
actatcaaaa ccaccatatt tttttggatc ccagtctttt ttacgagcaa taagcttgtc 6120
cgaatttctt tttggtaaaa ttgactcctt ggagaatccg cctgtctgta cttctgtttt 6180
cttgacaata ttgacttggg gcatggacaa tactttgcgc actgtggcaa aatctcgccc 6240
tttatcccag acaatttctc cagtttcccc attagtttcg attagagggc gtttgcgaat 6300
ctctccattt gcaagtgtaa tttctgtttt gaagaagttc atgatattag agtaaaagaa 6360
atattttgcg gttgctttgc ctatttcttg ctcagactta gcaatcattt tacgaacatc 6420
ataaacttta taatcaccat agacaaactc cgattcaagt tttggatatt tcttaatcaa 6480
agcagttcca acgacggcat ttagatacgc atcatgggca tgatggtaat tgttaatctc 6540
acgtacttta tagaattgga aatcttttcg gaagtcagaa actaatttag attttaaggt 6600
aatcacttta acctctcgaa taagtttatc attttcatcg tatttagtat tcatgcgact 6660
atccaaaatt tgtgccacat gcttagtgat ttggcgagtt tcaaccaatt ggcgtttgat 6720
aaaaccagct ttatcaagtt cactcaaacc tccacgttca gctttcgtta aattatcaaa 6780
cttacgttga gtgattaact tggcgtttag aagttgtctc caatagtttt tcatcttttt 6840
gactacttct tcacttggaa cgttatccga tttaccacga tttttatcag aacgcgttaa 6900
gaccttattg tctattgaat cgtctttaag gaaactttgt ggaacaatgt gatcgacatc 6960
ataatcactt aaacgattaa tatctaattc ttggtccaca tacatgtctc ttccattttg 7020
gagataatag agatagagct tttcattttg caattgagta ttttcaacag gatgctcttt 7080
aagaatctga cttcctaatt ctttgatacc ttcttcgatt cgtttcatac gctctcgcga 7140
atttttctgg cccttttgag ttgtctgatt ttcacgtgcc atttcaataa cgatattttc 7200
tggcttatgc cgccccatta ctttgaccaa ttcatcaaca acttttacag tctgtaaaat 7260
acctttttta atagcagggc taccagctaa atttgcaata tgttcatgta aactatcgcc 7320
ttgtccagac acttgtgctt tttgaatgtc ttctttaaat gtcaaactat catcatggat 7380
cagctgcata aaattgcgat tggcaaaacc atctgatttc aaaaaatcta atattgtttt 7440
gccagattgc ttatccctaa taccattaat caattttcga gacaaacgtc cccaaccagt 7500
ataacggcga cgtttaagct gtttcatcac cttatcatca aagaggtgag catatgtttt 7560
aagtctttcc tcaatcatct ccctatcttc aaataaggtc aatgttaaaa caatatcctc 7620
taagatatct tcattttctt cattatccaa aaaatcttta tctttaataa tttttagcaa 7680
atcatggtag gtacctaatg aagcattaaa tctatcttca actcctgaaa tttcaacact 7740
atcaaaacat tctatttttt tgaaataatc ttcttttaat tgcttaacgg ttacttttcg 7800
atttgttttg aagagtaaat caacaatggc tttcttctgt tcacctgaaa gaaatgctgg 7860
ttttcgcatt ccttcagtaa catatttgac ctttgtcaat tcgttataaa ccgtaaaata 7920
ctcataaagc aaactatgtt ttggtagtac tttttcattt ggaagatttt tatcaaagtt 7980
tgtcatgcgt tcaataaatg attgagctga agcaccttta tcgacaactt cttcaaaatt 8040
ccatggggta attgtttctt cagacttccg agtcatccat gcaaaacgac tattgccacg 8100
cgccaatgga ccaacataat aaggaattcg aaaagtcaag attttttcaa tcttctcacg 8160
attgtctttt aaaaatggat aaaagtcttc ttgtcttctc aaaatagcat gcagctcacc 8220
caagtgaatt tgatgggtaa tagagccgtt gtcaaaggtc cgttgcttgc gcagcaaatc 8280
ttcacgattt agtttcacca ataattcctc agtaccatcc attttttcta aaattggttt 8340
gataaattta taaaattctt cttggctagc tcccccatca atataacctg catatccgtt 8400
ttttgattga tcaaaaaaga tttctttata cttttctgga agttgttgtc gaactaaagc 8460
ttttaaaaga gtcaagtctt gatgatgttc atcgtagcgt ttaatcattg aagctgatag 8520
gggagcctta gttatttcag tatttactct taggatatct gaaagtaaaa tagcatctga 8580
taaattctta gctgccaaaa acaaatcagc atattgatct ccaatttgcg ccaataaatt 8640
atctaaatca tcatcgtaag tatcttttga aagctgtaat ttagcatctt ctgccaaatc 8700
aaaatttgat ttaaaattag gggtcaaacc caatgacaaa gcaatgagat tcccaaataa 8760
gccatttttc ttctcaccgg ggagctgagc aatgagattt tctaatcgtc ttgatttact 8820
caatcgtgca gaaagaatcg ctttagcatc tactccactt gcgttaatag ggttttcttc 8880
aaataattga ttgtaggttt gtaccaactg gataaatagt ttgtccacat cactattatc 8940
aggatttaaa tctccctcaa tcaaaaaatg accacgaaac ttaatcatat gcgctaaggc 9000
caaatagatt aagcgcaaat ccgctttata agtagaatct accaattttt ttcgcagatg 9060
atagatagtt ggatatttct catgataagc aacttcatct actatatttc caaaaatagg 9120
atgacgttca tgcttcttgt cttcttccac caaaaaagac tcttcaagtc gatgaaagaa 9180
actatcatct actttcgcca tctcatttga aaaaatctcc tgtagataac aaatacgatt 9240
cttccgacgt gtataccttc tacgagctgt ccgtttgaga cgagtcgctt ccgctgtctc 9300
tccactgtca aataaaagag cccctataag attttttttg atactgtggc ggtctgtatt 9360
tcccagaacc ttgaactttt tagacggaac cttatattca tcagtgatca ccgcccatcc 9420
gacgctattt gtgccgatat ctaagcctat tgagtatttc ttatcgacct ttctcttctt 9480
ttttggaggt ttgtcatcgt catctttata atccatctag aaaacttaga ttagattgct 9540
atgctttctt tctaatgagc aagaagtaaa aaaagttgta atagaacaag aaaaatgaaa 9600
ctgaaacttg agaaattgaa gaccgtttat taacttaaat atcaatggga ggtcatcgaa 9660
agagaaaaaa atcaaaaaaa aaattttcaa gaaaaagaaa cgtgataaaa atttttattg 9720
cctttttcga cgaagaaaaa gaaacgaggc ggcctctttt ttcttttcca aacctttagt 9780
acgggtaatt aacgacaccc tagaggaaga aagaggggaa atttagtatg ctgtgcttgg 9840
gtgttttgaa gtggtacggc gatgcgcgga gtccgagaaa atctggaaga gtaaaaaagg 9900
agtagaaaca ttttgaagat c 9921
<210> 15
<211> 9377
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 15
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420
cctctagaga ttgtttaaac tttcctctaa tcaggttcca ccaaacagat accccggtgt 480
ttcacggaat ggtacgtttg atatcgctga tttgagagga ggttacactt gaagaatcac 540
agtcttgcga ccggctattc aacaaggcat tcccccaagt ttgaattctt tgaaatagat 600
tgctattagc tagtaatcca ccaaatcctt cgctgctcac caatggaatc gcaagatgcc 660
cacgatgaga ctgttcaggt taaacgcaaa agaaacacac tctgggaatt tcttcccaaa 720
ttgtatctct caatacgcat caacccatgt caattaaaca cgctgtatag agactaggca 780
gatctgacga tcacctagcg actctctcca ccgtttgacg aggccattta caaaaacata 840
acgaacgaca agcctactcg aattcgtttc caaactcttt tcgaacttgt cttcaactgc 900
tttcgcatga agtacctccc aactactttt cctcacactt gtactccatg actaaacccc 960
ccctcccatt acaaactaaa atcttacttt tattttcttt tgccctctct gtcgctctgc 1020
cttaactacg tatttctcgc cgagaaaaac ttcaatttaa gctattctcc aaaaatctta 1080
gcgtatattt tttttccaaa gtgagcaaat taaagccttc gagcgtccca aaaccttctc 1140
aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ttgtacagaa aaaaaagaaa 1200
aatttgaaat ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac 1260
ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg ggggagggcg 1320
tgaatgtaag cgtgacataa ctaattacat gatatcgaca aaggaaaagg ggcctgttta 1380
tattgccagc gtcgatgttg atgacaacgc agagttcata cggttttggt tttgggcttc 1440
acttgtctca gtaggttgag ggtttgtatg aagtttcaga ggctccgtta gtaaatgacc 1500
cattctttct atcttggtcc tgagataacc ttctatctcc ttggagtcta tgccttcact 1560
ggagtttgtc cagtgtatgg gtaccatagg cactcgttca acgcacttaa ggtaaggcgc 1620
atgatctacc tgttttatct tttccgggtt atttgtcagc agcctgacat taccgatgcc 1680
aagatccagc aaaatagcct tacccagcga gaagtcccta gcatccacgg gatgtttcag 1740
cattaagttg gcctgcactg tgtcagcacc taagtcttgc aggttgtagg ccttgagttt 1800
ctcacctaac ccgatgccac gaccctcttg tcttagatac acgataacac catggccgtt 1860
tccacctttg atgttgcttg tgggttcgtg gtcgcaagcg ataagcctac cggccctatc 1920
gaattgttca ccacaatcac aacgggcgct ccatgcgttt tcaccggtgt aacattcaga 1980
atggatccgt accagcgtgt cgttatgggc gtcccatgta gtggctttgg aagctaataa 2040
ctcacctgta ctatcatcaa actccagagc taatccgagt ctatcgtctt cgtctgccac 2100
agttctgccg ggatacagtt tgccaatata agcgcccctg atcattctat cttgttgcgt 2160
ctcgcactgt cttctacgga atagcgagcg cgaccgtatg tcttcaccaa acacaatggc 2220
tagatgttcc ttgttgtccc tgttgttact gtaaagatgt aaaaagatat ccggaccctg 2280
tgtggttggg atacgagctc ttgcgacaca ttgtactaga ggcaagccgc catcgccgtt 2340
cctgccatca cccgtaccac taacctcgta tttgctgcta tcctgtttac tgttgtcgta 2400
gttatctatg gtcattatag ttttttctcc ttgacgttaa agtatagagg tatattaaca 2460
attttttgtt gatactttta tgacatttga ataagaagta atacaaaccg aaaatgttga 2520
aagtattagt taaagtggtt atgcagcttt tgcatttata tatctgttaa tagatcaaaa 2580
atcatcgctt cgctgattaa ttaccccaga aataaggcta aaaaactaat cgcattatta 2640
tcctatggtt gttaatttga ttcgttgatt tgaaggtttg tggggccagg ttactgccaa 2700
tttttcctct tcataaccat aaaagctagt attgtagaat ctttattgtt cggagcagtg 2760
cggcgcgagg cacatctgcg tttcaggaac gcgaccggtg aagaccagga cgcacggagg 2820
agagtcttcc gtcggagggc tgtcgcccgc tcggcggctt ctaatccgta cttcaatata 2880
gcaatgagca gttaagcgta ttactgaaag ttccaaagag aaggtttttt taggctaaga 2940
taatggggct ctttacattt ccacaacata taagtaagat tagatatgga tatgtatatg 3000
gtggtattgc catgtaatat gattattaaa cttctttgcg tccatccaaa aaaaaagtaa 3060
gaatttttga aaattcaata taaatgtctt tgacaccact gtgtgaagat ttaccacaat 3120
ttctgcaaaa ctatctaccg aatgctggtc aaacggaaaa taccattgtg ccctttgtca 3180
cactaactta tgctcaatcg ctcgacgcga gagtatctag gggccctgga gtgaggacta 3240
caatttcaca tcccgagacc aaaacaatga cgcattattt gagacatcat cacgatggaa 3300
tactcgtagg aagtggaaca gtgctagctg ataatcctgg attgaattgt aaatggggtc 3360
ccgatccggc tgcaaattcc ccaaggccaa taataataga tacaaagcaa aagtggcgat 3420
ttgatggttc aaaaatgcaa gaacttttta ttaaacgaca gggtaagccg ccaatcgttg 3480
ttgtcacaag tgagcccatt ataaaagaac aacatgtaga ctacgcaatt tgtccaataa 3540
atgatactac gaaattggtc gattggaaga aattgtttga gatattaaaa gaagaattca 3600
atataaggtc agtaatggtt gaaggaggtg ccaatgtaat aaatcagttg ttgctgagga 3660
gcgatattgt caacagtctt ataataacta ttggatcaac atttctgggt agctcaggca 3720
ccgaagttag cccaccccaa acagtaaatt taaaggatat gtcatggtgg aagggcatta 3780
ccgatgtggt gctttgtgcg agactggccg atgactaatt tgccagctta ctatccttct 3840
tgaaaatatg cactctatat cttttagttc ttaattgcaa cacatagatt tgctgtataa 3900
cgaattttat gctatttttt aaatttggag ttcagtgata aaagtgtcac agcgaatttc 3960
ctcacatgta gggaccgaat tgtttacaag ttctctgtac caccatggag acatcaaaga 4020
ttgaaaatct atggaaagat atggacggta gcaacaagaa tatagcacga gccgcgaagt 4080
tcatttcgtt acttttgata tcgctcacaa ctattgcgaa gcgcttcagt gaaaaaatca 4140
taaggaaaag ttgtaaatat tattggtagt attcgtttgg taaagtagag ggggtaattt 4200
ttccccttta ttttgttcat acattcttaa attgctttgc ctctcctttt ggaaagctat 4260
acttcggagc actgttgagc gaaggctcat tagatatatt ttctgtcatt ttccttaacc 4320
caaaaataag ggaaagggtc caaaaagcgc tcggacaact gttgaccgtg atccgaagga 4380
ctggctatac agtgttcaca aaatagccaa gctgaaaata atgtgtagct atgttcagtt 4440
agtttggcta gcaaagatat aaaagcaggt cggaaatatt tatgggcatt attatgcaga 4500
gcatcaacat gataaaaaaa aacagttgaa tattccctca aaaatggagg actctaataa 4560
tgaagccagc gatgacttca acaacttatt aaacaaagaa attgagtctg caaaggaagt 4620
gaagttaaga aaattcgcga acaggaataa taataggaac gaaaacagtt ccaaagtcaa 4680
agatgcgagt ggattcagac tgagagttat tcaaaccgat gggcacaaaa ctaaaaagac 4740
agaccctgat tatgaagtga caattgatgg acccttaagg aagattgagc cttatttttt 4800
cacttataaa actttctgta aggagaggtg gagagatcgt aagttggtgg atgtattcgt 4860
gagcgaattc agggatcgag agcccagcta ttattccaaa accattgcag agggaaaagt 4920
atacttaaac gatgaacctg caaaccttga taccatcatt cgtgacggtg atctgatcac 4980
acataaagta catcgacatg aaccgccagt cacatctaaa ccaatagata ttgtgtttga 5040
agacgaagat atccttgtca ttgataaacc cagcagtatt ccagtgcacc caacgggcag 5100
atacagattc aataccatta caaaaatgct agaaagacag ctaggttatt cagtacatcc 5160
atgtaataga ctagacaagc caactagtgg actaatgttt ttagccaaga ctccattagg 5220
tgcagataga atgggagacc aaatgaaagc gagggaagtc accaaagaat acgtagcccg 5280
cgtgaagggc gagttcccta taggtatagt agaggtagat aagcctgtca gatctgttaa 5340
ccctaaagtt gcactaaacg ctgtttgcga gatgagcgat gaaaacgcta aacatgccaa 5400
gaccgttttc caaagggtta gctatgatgg acagacgagt attgtaaagt gtaaaccatt 5460
gacaggtaga actcaccaaa taagagtcca tttgcagtac ttgggatttc ccatagcaaa 5520
tgaccctata tactcgaacc cagatatctg gggtccagat ttgggccgcg gtggacttca 5580
aaactatgat gacatcgttc tgaaactaga cgctattggc aagactaatc ctgcagagag 5640
ctggattcat cctcatagcg agggtgaata cttgcttggc cgtcagtgcg aagaatgcga 5700
ggctgaaatg tacacagatc ccggtactaa tgatctcgac ctctggctgc atgccttccg 5760
gtacgagtca ttggaaagaa attcggatac gcaaaaacct ctctggagtt atagaacaaa 5820
ataccctgaa tgggccttag agccacatcg ccgatatatg gaaatggccg ttaaggaagc 5880
tggtaagtgt ggcccgacaa agactgcttt tagtgttggt gccgttcttg ttcatggaac 5940
tcaagtactt gccacaggat attcaagaga gctaccagga aacactcatg cagaacagtg 6000
tgctttgata aagtactcgc agttacaccc gaactgtccc actatagtcc ctatgggaac 6060
agtgctctat acaaccatgg aaccctgttc cttcaggcta agtggcaatg agccttgctg 6120
cgatagaatc ctggccaccc aaggtgccat tggtactgtc ttcgtggggg ttatggagcc 6180
cgatacattt gttaaaaaca atacaagttt gaacaagctg gaatcgcacg gtgtgaacta 6240
catacaaata ccaggctacg aggaggagtg caccatcatt gccttcaaag gccacgataa 6300
tagtgacgac aaagcttagg cgaatttctt atgatttatg atttttatta ttaaataagt 6360
tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa aacgaaaatt 6420
cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat agcatgaggt 6480
cgctcttatt gaccacacct ctaccggcag gtgccccggg taacccagtt cctcactatt 6540
ttttactgcg gaagcggaag cggaaaatac ggaaacgcgc gggaacatac aaaacataca 6600
aaatatacct ttctcacaca agaaatatat gctacttgca aaatatcata ccaaaaaact 6660
tttcacaacc gaaaccaaaa ccaacggata tcatacatta cactaccacc attcaaactt 6720
tactactatc ctcccttcag tttccctttt tctgcctttt tcggtgacgg aaatacgctt 6780
cagagaccct aaagggaaat ccatgccata acaggaaagt aacatcccaa tgcggactat 6840
accaccccac cacactccta ccaataacgg taactattct atgttttctt actcctatgt 6900
ctattcatct ttcatctgac tacctaatac tatgcaaaaa tgtaaaatca tcacacaaaa 6960
cataaacaat caaaatcagc catttccgca ccttttcctc tgtccacttt caaccgtccc 7020
tccaaatgta aaatggccta tcggaataca ttttctacat cctaactact ataaaacaac 7080
ctttagactt acgtttgcta ctctcatggt ttaaacatcg tcgacctgca ggcatgcaag 7140
cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 7200
acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 7260
actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 7320
gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 7380
cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 7440
tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 7500
gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 7560
ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 7620
aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 7680
tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 7740
ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 7800
gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 7860
tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 7920
caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 7980
ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt 8040
cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 8100
ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 8160
cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 8220
gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 8280
aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 8340
acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 8400
gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 8460
cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 8520
cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 8580
tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 8640
cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 8700
gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 8760
cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 8820
ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 8880
gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 8940
taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 9000
gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 9060
acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 9120
aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 9180
cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 9240
atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 9300
gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 9360
cacgaggccc tttcgtc 9377
<210> 16
<211> 7682
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 16
tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 60
cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 120
ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 180
accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 240
attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 300
tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 360
tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccggggat 420
cctctagaga ttgtttaaac tttcctctaa tcaggttcca ccaaacagat accccggtgt 480
ttcacggaat ggtacgtttg atatcgctga tttgagagga ggttacactt gaagaatcac 540
agtcttgcga ccggctattc aacaaggcat tcccccaagt ttgaattctt tgaaatagat 600
tgctattagc tagtaatcca ccaaatcctt cgctgctcac caatggaatc gcaagatgcc 660
cacgatgaga ctgttcaggt taaacgcaaa agaaacacac tctgggaatt tcttcccaaa 720
ttgtatctct caatacgcat caacccatgt caattaaaca cgctgtatag agactaggca 780
gatctgacga tcacctagcg actctctcca ccgtttgacg aggccattta caaaaacata 840
acgaacgaca agcctactcg aattcgtttc caaactcttt tcgaacttgt cttcaactgc 900
tttcgcatga agtacctccc aactactttt cctcacactt gtactccatg actaaacccc 960
ccctcccatt acaaactaaa atcttacttt tattttcttt tgccctctct gtcgctctgc 1020
cttaactacg tatttctcgc cgagaaaaac ttcaatttaa gctattctcc aaaaatctta 1080
gcgtatattt tttttccaaa gtgagcaaat taaagccttc gagcgtccca aaaccttctc 1140
aagcaaggtt ttcagtataa tgttacatgc gtacacgcgt ttgtacagaa aaaaaagaaa 1200
aatttgaaat ataaataacg ttcttaatac taacataact ataaaaaaat aaatagggac 1260
ctagacttca ggttgtctaa ctccttcctt ttcggttaga gcggatgtgg ggggagggcg 1320
tgaatgtaag cgtgacataa ctaattacat gatatcgaca aaggaaaagg ggcctgttta 1380
tttcttcaaa tattgggcca attcttcgat ggagatcaaa ggaatgccat gtttcttacc 1440
aaacgcttgg cagtcattta atctcatcat agttccttgt tcgtcatcgt taaccaattc 1500
gccaataaca gcgacgggac ttagtccact tagtttacac aaatcgacac cggcctcagt 1560
gtggcctctt ctctgcaaaa caccgccatc agcggctctc aagggacaga tgtgccctgg 1620
ttttaaaaat gattttggcg tagaggaaga gtctgcaaga gccctacaag tcatcgacct 1680
gtcgtgagca gaaatacctg tggtagtgcc ttgggctaca tctacagtta ttgtgtatgc 1740
agttccatgc ctgtcgtcat cattggattc aaacttcatg cctgttctca gtagtggaag 1800
gtccaactta tcagcaatgg cattcgtcat aggtgcacag acgtagcctg aggaatggcg 1860
tacaagaaag gccatttgtt cagtgctgac attttcagca gcgcaaataa gatcaccttc 1920
attttcacga ccggcatcgt ccataacaat tacaaactta ttctgcttga agtgttctat 1980
agcttgatca attggtgtaa acattatagt tttttctcct tgacgttaaa gtatagaggt 2040
atattaacaa ttttttgttg atacttttat gacatttgaa taagaagtaa tacaaaccga 2100
aaatgttgaa agtattagtt aaagtggtta tgcagctttt gcatttatat atctgttaat 2160
agatcaaaaa tcatcgcttc gctgattaat taccccagaa ataaggctaa aaaactaatc 2220
gcattattat cctatggttg ttaatttgat tcgttgattt gaaggtttgt ggggccaggt 2280
tactgccaat ttttcctctt cataaccata aaagctagta ttgtagaatc tttattgttc 2340
ggagcagtgc ggcgcgaggc acatctgcgt ttcaggaacg cgaccggtga agaccaggac 2400
gcacggagga gagtcttccg tcggagggct gtcgcccgct cggcggcttc taatccgtac 2460
ttcaatatag caatgagcag ttaagcgtat tactgaaagt tccaaagaga aggttttttt 2520
aggctaagat aatggggctc tttacatttc cacaacatat aagtaagatt agatatggat 2580
atgtatatgg tggtattgcc atgtaatatg attattaaac ttctttgcgt ccatccaaaa 2640
aaaaagtaag aatttttgaa aattcaatat aaatggcagt taaaggatta ggcaaaccag 2700
atcaagttta tgacggttcc aaaatcaggg tcggtatcat tcatgcccgt tggaaccgtg 2760
tcattattga cgctcttgtg aaaggtgcta ttgaaagaat ggcttccctt ggtgtcgagg 2820
aaaataacat aataattgaa actgttcctg gatcctatga attaccttgg gggacaaaaa 2880
ggtttgttga cagacaagcg aagttgggaa agccgctaga tgttgtaatt cctattggtg 2940
tacttatcaa aggtagtaca atgcactttg aatacatttc agattccacc actcacgcat 3000
tgatgaactt acaagaaaaa gtcgacatgc ccgtcatttt tggcctctta acttgcatga 3060
ctgaagaaca agccctggcc agagctggca tcgatgaagc gcactccatg cacaaccatg 3120
gtgaagattg gggtgctgca gcagtggaaa tggctgttaa gttcggtaaa aatgcttttt 3180
gatttgccag cttactatcc ttcttgaaaa tatgcactct atatctttta gttcttaatt 3240
gcaacacata gatttgctgt ataacgaatt ttatgctatt ttttaaattt ggagttcagt 3300
gataaaagtg tcacagcgaa tttcctcaca tgtagggacc gaattgttta caagttctct 3360
gtaccaccat ggagacatca aagattgaaa atctatggaa agatatggac ggtagcaaca 3420
agaatatagc acgagccgcg aagttcattt cgttactttt gatatcgctc acaactattg 3480
cgaagcgctt cagtgaaaaa atcataagga aaagttgtaa atattattgg tagtattcgt 3540
ttggtaaagt agagggggta atttttcccc tttattttgt tcatacattc ttaaattgct 3600
ttgcctctcc ttttggaaag ctatacttcg gagcactgtt gagcgaaggc tcattagata 3660
tattttctgt cattttcctt aacccaaaaa taagggaaag ggtccaaaaa gcgctcggac 3720
aactgttgac cgtgatccga aggactggct atacagtgtt cacaaaatag ccaagctgaa 3780
aataatgtgt agctatgttc agttagtttg gctagcaaag atataaaagc aggtcggaaa 3840
tatttatggg cattattatg cagagcatca acatgataaa aaaaaacagt tgaatattcc 3900
ctcaaaaatg tttactggta ttgtagaatg catggggact gttttggaaa acaacccata 3960
tgatgactct gaaagtggag gtcaaggagt ttctattact attggcaatg cggggagtat 4020
tctcaccgat tgtcacgttg gagattcaat agccgtaaat ggggtatgcc ttactgtgac 4080
cgagtttaat aacgactcct tcaaagttgg gatatcacca gaaactataa aacgaagtaa 4140
tgtcgcttcc tggattcaag gcacccaggt caacttggag agagcggtat ctcaagacgt 4200
taggttcggt ggtcattatg tacagggtca cgtagacact gttgctaata ttgtctcaag 4260
aagacctgag gggaattcaa ttatttttgg gtttcagtta agagatcaag agtactttaa 4320
atacatagta gaaaagggat tcatttgtat agatggaact tccttgacca taatcaaggt 4380
tgacccactt tcgcaaggtg gagccttcta tattagtatg ataaagcaca cccaagacaa 4440
tgttatcatg cctttgaaga aaattggcga cgaggttaat attgaagtag atttgactgg 4500
gaagattatt gagaagcaaa ttctattaac gttggaaaac caaatatcaa agaaagatag 4560
tactttgaat actatgatct caaacattat cgaggagaag gttagaaact acctaaataa 4620
ataagcgaat ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag 4680
tgtatacaaa ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa 4740
ctctttcctg taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca 4800
cacctctacc ggcaggtgcc ccgggtaacc cagttcctca ctatttttta ctgcggaagc 4860
ggaagcggaa aatacggaaa cgcgcgggaa catacaaaac atacaaaata tacctttctc 4920
acacaagaaa tatatgctac ttgcaaaata tcataccaaa aaacttttca caaccgaaac 4980
caaaaccaac ggatatcata cattacacta ccaccattca aactttacta ctatcctccc 5040
ttcagtttcc ctttttctgc ctttttcggt gacggaaata cgcttcagag accctaaagg 5100
gaaatccatg ccataacagg aaagtaacat cccaatgcgg actataccac cccaccacac 5160
tcctaccaat aacggtaact attctatgtt ttcttactcc tatgtctatt catctttcat 5220
ctgactacct aatactatgc aaaaatgtaa aatcatcaca caaaacataa acaatcaaaa 5280
tcagccattt ccgcaccttt tcctctgtcc actttcaacc gtccctccaa atgtaaaatg 5340
gcctatcgga atacattttc tacatcctaa ctactataaa acaaccttta gacttacgtt 5400
tgctactctc atggtttaaa catcgtcgac ctgcaggcat gcaagcttgg cgtaatcatg 5460
gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc 5520
cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc 5580
gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat 5640
cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac 5700
tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt 5760
aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca 5820
gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc 5880
ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact 5940
ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct 6000
gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag 6060
ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca 6120
cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa 6180
cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc 6240
gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag 6300
aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg 6360
tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca 6420
gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc 6480
tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag 6540
gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata 6600
tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat 6660
ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg 6720
ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc 6780
tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc 6840
aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc 6900
gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc 6960
gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc 7020
ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa 7080
gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat 7140
gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata 7200
gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca 7260
tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag 7320
gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc 7380
agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc 7440
aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata 7500
ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 7560
gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 7620
agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 7680
tc 7682
<210> 17
<211> 9263
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 17
ggccgcgaca ttgaactctt ggccgactct tctgatgagg ctttcgaaaa atctactttc 60
attaacacaa ttgagttcat tgtgcacgat atttgggtcg aaacgttgat aaagaacata 120
aacttattac aaatgtttga tgcagatttg aaatgctaca atgacaagta cataatttgc 180
aagctaaagg gtcagtatcc ctccacaaat atagtggaca taatgtgccg tttgaagcat 240
ttttccaatt ctattttgga aactttcaaa ttcggaatcg agctaaaaga acaagatcaa 300
tgccacaata gaaacactat tatcaactac gttcttttca gtagagtatt ttcaacgatt 360
gttttagaga ttcaaaaatg ctttatcttg atcgttaagt tcatgtattc tgtcaatttt 420
ttagaaaaat tttccgatga agtatttttg tcatttattg aaattctcat aaaaattgtc 480
tttgagcacc agattccgca gttatttttg ggaattgatg aaattattca actctggtta 540
aagaacaatg aaggaaaacg gcaacaactc ttgagcgctt ggtgcaacgg aactgttcaa 600
gatatgaagc aaagccaaca aagagagtcc tcaaacgcag aatctgaatc aatcacttca 660
agtacagaag aggacgaaga aggcctgcaa ttcaataaat gggatgtgat tgaaccattt 720
attgataata tcaaggcttt gaatcaatca aaatcccata tgtgacgcag cgataaaacc 780
gcggcatgca aacatctaca caattagcaa gggcaatcca tattttgtct tttcgcgccc 840
tggaaaggcc taagtaatgt cgtaaacgca ttctatctgt acttcaactc tcctctgtgc 900
attggtttgt gcaaatcaca ttttacgata ctgccagata tatgcaaaaa gagaaaacca 960
agggaccaga acaaagcaaa attacgatat tcttcgaatt ccttcgtgct tgactaagac 1020
aaagggatgg acgtagcgat ttttagcggg ccaagaactg gttccgaaaa agcacaggta 1080
caccgaaccc tcagctaagg agggacagca ccgatgcgga aggacaaact ttctttttgc 1140
ctatcacagt atcttatcga gctaactatt ttcgacacac atgaaaaagc agaaatatta 1200
acgaaaaaga aaagaaagac catgtcatgt acgggcaatc agaatctgta acaagcgcca 1260
tttttttttc tgtatcgggc cctccttact gctctccttc cgtgtaacgc gttatggctg 1320
caggtcgaca acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtct 1380
agagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac atggaggccc 1440
agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc 1500
cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat tttgatggcc 1560
gcacggcgcg aagcaaaaat tacggctcct cgctgcagac ctgcgagcag ggaaacgctc 1620
ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt 1680
aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg ctaggataca 1740
gttctcacat cacatccgaa cataaacaac catgggtaaa aagcctgaac tcaccgcgac 1800
gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga tgcagctctc 1860
ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat atgtcctgcg 1920
ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc actttgcatc 1980
ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga gcctgaccta 2040
ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa ccgaactgcc 2100
cgctgttctg cagccggtcg cggaggcaat ggatgcgatc gctgcggccg atcttagcca 2160
gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta catggcgtga 2220
tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga tggacgacac 2280
cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg aggactgccc 2340
cgaagtccgg cacctcgtgc acgcggattt cggctccaac aatgtcctga cggacaatgg 2400
ccgcataaca gcggtcattg actggagcga ggcgatgttc ggggattccc aatacgaggt 2460
cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga cgcgctactt 2520
cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata tgctccgcat 2580
tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg cagcttgggc 2640
gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc gtacacaaat 2700
cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg ccgatagtgg 2760
aaaccgacgc cccagcactc gtccgagggc aaaggaataa tcagtactga caataaaaag 2820
attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt tgttctattt 2880
taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg cccagatgcg 2940
aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct ggtcgctata 3000
ctgctgtcga ttcgatacta acgagctcat agcttcaaaa tgtttctact ccttttttac 3060
tcttccagat tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag 3120
catactaaat ttcccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt 3180
tggaaaagaa aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat 3240
ttttatcacg tttctttttc ttgaaaattt tttttttgat ttttttctct ttcgatgacc 3300
tcccattgat atttaagtta ataaacggtc ttcaatttct caagtttcag tttcattttt 3360
cttgttctat tacaactttt tttacttctt gctcattaga aagaaagcat agcaatctaa 3420
tctaagtttt ctagaactag tggatccccc gggaaaaatg gacaagaagt actccattgg 3480
gctcgatatc ggcacaaaca gcgtcggttg ggccgtcatt acggacgagt acaaggtgcc 3540
gagcaaaaaa ttcaaagttc tgggcaatac cgatcgccac agcataaaga agaacctcat 3600
tggcgccctc ctgttcgact ccggggagac ggccgaagcc acgcggctca aaagaacagc 3660
acggcgcaga tatacccgca gaaagaatcg gatctgctac ctgcaggaga tctttagtaa 3720
tgagatggct aaggtggatg actctttctt ccataggctg gaggagtcct ttttggtgga 3780
ggaggataaa aagcacgagc gccacccaat ctttggcaat atcgtggacg aggtggcgta 3840
ccatgaaaag tacccaacca tatatcatct gaggaagaag cttgtagaca gtactgataa 3900
ggctgacttg cggttgatct atctcgcgct ggcgcatatg atcaaatttc ggggacactt 3960
cctcatcgag ggggacctga acccagacaa cagcgatgtc gacaaactct ttatccaact 4020
ggttcagact tacaatcagc ttttcgaaga gaacccgatc aacgcatccg gagttgacgc 4080
caaagcaatc ctgagcgcta ggctgtccaa atcccggcgg ctcgaaaacc tcatcgcaca 4140
gctccctggg gagaagaaga acggcctgtt tggtaatctt atcgccctgt cactcgggct 4200
gacccccaac tttaaatcta acttcgacct ggccgaagat gccaagcttc aactgagcaa 4260
agacacctac gatgatgatc tcgacaatct gctggcccag atcggcgacc agtacgcaga 4320
cctttttttg gcggcaaaga acctgtcaga cgccattctg ctgagtgata ttctgcgagt 4380
gaacacggag atcaccaaag ctccgctgag cgctagtatg atcaagcgct atgatgagca 4440
ccaccaagac ttgactttgc tgaaggccct tgtcagacag caactgcctg agaagtacaa 4500
ggaaattttc ttcgatcagt ctaaaaatgg ctacgccgga tacattgacg gcggagcaag 4560
ccaggaggaa ttttacaaat ttattaagcc catcttggaa aaaatggacg gcaccgagga 4620
gctgctggta aagcttaaca gagaagatct gttgcgcaaa cagcgcactt tcgacaatgg 4680
aagcatcccc caccagattc acctgggcga actgcacgct atcctcaggc ggcaagagga 4740
tttctacccc tttttgaaag ataacaggga aaagattgag aaaatcctca catttcggat 4800
accctactat gtaggccccc tcgcccgggg aaattccaga ttcgcgtgga tgactcgcaa 4860
atcagaagag accatcactc cctggaactt cgaggaagtc gtggataagg gggcctctgc 4920
ccagtccttc atcgaaagga tgactaactt tgataaaaat ctgcctaacg aaaaggtgct 4980
tcctaaacac tctctgctgt acgagtactt cacagtttat aacgagctca ccaaggtcaa 5040
atacgtcaca gaagggatga gaaagccagc attcctgtct ggagagcaga agaaagctat 5100
cgtggacctc ctcttcaaga cgaaccggaa agttaccgtg aaacagctca aagaagacta 5160
tttcaaaaag attgaatgtt tcgactctgt tgaaatcagc ggagtggagg atcgcttcaa 5220
cgcatccctg ggaacgtatc acgatctcct gaaaatcatt aaagacaagg acttcctgga 5280
caatgaggag aacgaggaca ttcttgagga cattgtcctc acccttacgt tgtttgaaga 5340
tagggagatg attgaagaac gcttgaaaac ttacgctcat ctcttcgacg acaaagtcat 5400
gaaacagctc aagaggcgcc gatatacagg atgggggcgg ctgtcaagaa aactgatcaa 5460
tgggatccga gacaagcaga gtggaaagac aatcctggat tttcttaagt ccgatggatt 5520
tgccaaccgg aacttcatgc agttgatcca tgatgactct ctcaccttta aggaggacat 5580
ccagaaagca caagtttctg gccaggggga cagtcttcac gagcacatcg ctaatcttgc 5640
aggtagccca gctatcaaaa agggaatact gcagaccgtt aaggtcgtgg atgaactcgt 5700
caaagtaatg ggaaggcata agcccgagaa tatcgttatc gagatggccc gagagaacca 5760
aactacccag aagggacaga agaacagtag ggaaaggatg aagaggattg aagagggtat 5820
aaaagaactg gggtcccaaa tccttaagga acacccagtt gaaaacaccc agcttcagaa 5880
tgagaagctc tacctgtact acctgcagaa cggcagggac atgtacgtgg atcaggaact 5940
ggacatcaat cggctctccg actacgacgt ggatcatatc gtgccccagt cttttctcaa 6000
agatgattct attgataata aagtgttgac aagatccgat aaaaatagag ggaagagtga 6060
taacgtcccc tcagaagaag ttgtcaagaa aatgaaaaat tattggcggc agctgctgaa 6120
cgccaaactg atcacacaac ggaagttcga taatctgact aaggctgaac gaggtggcct 6180
gtctgagttg gataaagccg gcttcatcaa aaggcagctt gttgagacac gccagatcac 6240
caagcacgtg gcccaaattc tcgattcacg catgaacacc aagtacgatg aaaatgacaa 6300
actgattcga gaggtgaaag ttattactct gaagtctaag ctggtctcag atttcagaaa 6360
ggactttcag ttttataagg tgagagagat caacaattac caccatgcgc atgatgccta 6420
cctgaatgca gtggtaggca ctgcacttat caaaaaatat cccaagcttg aatctgaatt 6480
tgtttacgga gactataaag tgtacgatgt taggaaaatg atcgcaaagt ctgagcagga 6540
aataggcaag gccaccgcta agtacttctt ttacagcaat attatgaatt ttttcaagac 6600
cgagattaca ctggccaatg gagagattcg gaagcgacca cttatcgaaa caaacggaga 6660
aacaggagaa atcgtgtggg acaagggtag ggatttcgcg acagtccgga aggtcctgtc 6720
catgccgcag gtgaacatcg ttaaaaagac cgaagtacag accggaggct tctccaagga 6780
aagtatcctc ccgaaaagga acagcgacaa gctgatcgca cgcaaaaaag attgggaccc 6840
caagaaatac ggcggattcg attctcctac agtcgcttac agtgtactgg ttgtggccaa 6900
agtggagaaa gggaagtcta aaaaactcaa aagcgtcaag gaactgctgg gcatcacaat 6960
catggagcga tcaagcttcg aaaaaaaccc catcgacttt ctcgaggcga aaggatataa 7020
agaggtcaaa aaagacctca tcattaagct tcccaagtac tctctctttg agcttgaaaa 7080
cggccggaaa cgaatgctcg ctagtgcggg cgagctgcag aaaggtaacg agctggcact 7140
gccctctaaa tacgttaatt tcttgtatct ggccagccac tatgaaaagc tcaaagggtc 7200
tcccgaagat aatgagcaga agcagctgtt cgtggaacaa cacaaacact accttgatga 7260
gatcatcgag caaataagcg aattctccaa aagagtgatc ctcgccgacg ctaacctcga 7320
taaggtgctt tctgcttaca ataagcacag ggataagccc atcagggagc aggcagaaaa 7380
cattatccac ttgtttactc tgaccaactt gggcgcgcct gcagccttca agtacttcga 7440
caccaccata gacagaaagc ggtacacctc tacaaaggag gtcctggacg ccacactgat 7500
tcatcagtca attacggggc tctatgaaac aagaatcgac ctctctcagc tcggtggaga 7560
cagcagggct gaccccaaga agaagaggaa ggtgtgatct cttctcgagt catgtaatta 7620
gttatgtcac gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga 7680
gttagacaac ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga 7740
acgttattta tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac 7800
attatactga aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcccg 7860
ccatccagtg tcgaaaacga gctctcgaga acccttaata taacttcgta taatgtatgc 7920
tatacgaagt tattaggtga tatcagatcc actagtggct actatcggcg actctctcga 7980
aatttttctt aacgcgtcct tgtactgcgt ctaacgcttt tgccacttgg atttctatta 8040
taggaaatag tctcacttac tgggcgacga attttcgcgt tttgatgaag cacaggaaga 8100
atttcttttt tttttggctt cttctggttc cgttttttac gcgcacaaat ctaaaaaaag 8160
aaataattat aacctagtct cgaaaatttt catcgatcca ttcgttcctt tttttcgatt 8220
ttttcagatc aaaattcttg tttctttctt tgtcttagtt tatattaaaa gatattttga 8280
ttttactcct gaactattta ttctttctaa gaaggccaga acactacagc tgttttaacc 8340
gactacgaag ttctccattc tcgaacacta gccttcattt accaaacagg aactagcgta 8400
tatcattagt ccttattcga aaagagattg gtagatattt attgtagttt gtgagaagga 8460
gaaaatactg tcattggact gatagttaga ggacattaac ctctcttacg ttcgctcaaa 8520
aaaattaaaa taagcaagta gctgaaatca agtctttcat acaacggtca gaccatgaac 8580
actgatcaac aaccttatca gggccaaacg gactataccc agggaccagg taacgggcaa 8640
agtcaggaac aagactatga ccaatatggc cagcctttgt atccttcaca agctgatggt 8700
tactacgatc caaatgtcgc tgctggtact gaagctgata tgtatggtca acaaccacca 8760
aacgagtctt acgaccaaga ctacacaaac ggtgaatact atggtcaacc gccaaatatg 8820
gctgctcaag acggtgaaaa cttctcggat tttagcagtt acggccctcc tggaacacct 8880
ggatatgata gctatggtgg tcagtatacc gcttctcaaa tgagttatgg agaaccaaat 8940
tcgtcgggta cctcgactcc aatttacggt aattatgacc caaatgctat cgctatggct 9000
ttgccaaatg aaccttatcc cgcttggact gctgactctc aatctcccgt ttcgatcgag 9060
caaatcgaag atatctttat tgatttgacc aacagactcg ggttccaaag agactccatg 9120
agaaatatgt ttgatcattt tatggttctc ttggactcta ggtcctcgag aatgtctcct 9180
gatcaagctt tactatcttt acatgccgac tacattggtg gcgatactgc taactataaa 9240
aaatggtatt ttgctgctca ggc 9263
<210> 18
<211> 8424
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 18
ggccgcgcag agaatcctcg ggtttgtaaa ccatgttata gttaaacccc gatattacac 60
cattaaaatc atttctaaat atgcggtcgt cgatgatcag ctgcagggaa atgccaaaat 120
atggacccca tagaggtctt ggcttgttca tatcgttggg tcgatttctg ttcccagtgt 180
tatttggttg aatgggaaca gtcgcgtctt gccccatctt gtctctgccg ctttcgtcga 240
atctgacttt gcaattgacg ttgcatatca acgtgccgga tcccggaata gcgtgatagt 300
ctactcctgt tagggcatgt tgtgtttgta cgacctggtt ttgccacatt tgcagaaaaa 360
ctgttgcttg cgcgaagggg gtagcattaa atattattct gcagttgttt ggattaaaaa 420
gctgtacgaa ttgggccaat ttgttggagt ccggatcatc caaatgcgct aatatcttct 480
tcgtaaatgt ggccgttatt tgtgcttgat ttgcgtcatt cattaccata gtattactat 540
tggtgttcat atttctgtta ttactgttat tagtttggcg ttttgcctga cgatcagcct 600
ggtggggcca agtatatact ataaaaacag gttagttact tgctctatgc gtttgcgcat 660
cctcttttta cttttttttt ttcagtaaag cctaagcata aatcgtttta tacgtacgac 720
acgttcaact tttcttggtt agtagtggca atctctgcaa tacatacagg gagtcatggt 780
ctatcatctt gtccaatcaa agaagcatcg gttcagatcg agcaaactgt agggagaaag 840
gaaagtagaa atgcagagtg tgctatatgt ccaatctcgg ttttgtagtt tggatgtcat 900
tagagatcta ccacccaacc ggctgctttc atgtggaaca gaaaagaaat cggggcgctt 960
cctcttctgt attcctttaa ttaacgtttt tattcagcca tctaaccatc atacccccat 1020
acggtaacaa aacctcttct aagaaaagaa gtctctgctc ctccgccatc ttatttttat 1080
tcgctgcgcg cgtttattgt cgcatcgcta gccagcaaaa agttggttgc ctttttttac 1140
ctaaaaaaga cacatctaac tgattagttt tccgttttag gatattgacg ccaagcgtgc 1200
gtctgattgc aaattaaagc cttcgagcgt cccaaaacct tctcaagcaa ggttttcagt 1260
ataatgttac atgcgtacac gcgtttgtac agaaaaaaaa gaaaaatttg aaatataaat 1320
aacgttctta atactaacat aactataaaa aaataaatag ggacctagac ttcaggttgt 1380
ctaactcctt ccttttcggt tagagcggat gtggggggag ggcgtgaatg taagcgtgac 1440
ataactaatt acatgatatc gacaaaggaa aaggggcctg tttatttatc aagataagtt 1500
tccggatctt tttctttcct aacaccccag tcagcctgag ttacatccag ccattgaacc 1560
ttagaaaatc ttttgtcatc agcggtttga gccctaagat caacatcttg cttagcaatc 1620
actgcaatgg cgtcataacc accagcacca ggtattaagc aagtaagaac tccttttaag 1680
gtctggcaat catccaataa gctagtttgt acgggaggtt cgatatcggc accagattct 1740
ttagttattt ttctaaagga acgtctaatt gtggcaactg catctctaac ttctgtgatc 1800
tcaggatact tttgacaggt acagtcattc ctctcaagag actcaaatat ctgatcgctg 1860
taatcgtcat gagtctcgtg taagcgatct agtttagata gtccatccat aaatctagaa 1920
tttgcatgat cgagttctgt atatattttc aagctttccg gcatatgcga atcataccaa 1980
ttttttacct tctggaccag ttttactgtt tctgaaccat tcttaatatc gcccatccat 2040
aaagttaatc ccgaaggtaa atggttactt ttaatcgtta tattccagtc ttcttcatta 2100
accaaatgcg ccagtttact gccgtaagta gcacttccaa tatctggcaa attagagatt 2160
aatgcgggtg ggaatcttct atatctgata gatccatatg ctgccgccgc tacatcaaac 2220
ccgcttccaa ttttaccctg agcttgacaa tgagcaactt gtgataaatt atgaataact 2280
tctctatatt tgtctacatt attttccagg tccgatacaa aaaaggaggc caaagctgta 2340
gttaaaactg tgactaaacc tgccgaggag cccagccctg ttttgggaac ttcttcaatt 2400
ctgtgcgaat gaaaactcaa tcttctgttg ccacgatgtt cggtaacgct gtcctcctga 2460
gaatggtagg catcatcaga gaaaatatca ataacgaaca agtttctatt gcagtagtcg 2520
tccatgttag gcttaaagta gctaaatacg ttagcgataa ctttttcaat gaaagggttc 2580
ttagatccgc ctatcgaaac aggaatgaag ccagttttag gacttatatg gtacagccac 2640
tccccatctt taaattgttt acttttcaca cgcacttcaa acttatcaga ctcttgcaat 2700
gaaccgtaag gatgggctac agcatgcatt cttgccgata atccgactac aaatgcttca 2760
tatttcggat ctaaaactaa atatccacca gctagtaacg ctttccctgg ggcactgaag 2820
gctctcaact ctgacattat agttttttct ccttgacgtt aaagtataga ggtatattaa 2880
caattttttg ttgatacttt tatgacattt gaataagaag taatacaaac cgaaaatgtt 2940
gaaagtatta gttaaagtgg ttatgcagct tttgcattta tatatctgtt aatagatcaa 3000
aaatcatcgc ttcgctgatt aattacccca gaaataaggc taaaaaacta atcgcattat 3060
tatcctatgg ttgttaattt gattcgttga tttgaaggtt tgtggggcca ggttactgcc 3120
aatttttcct cttcataacc ataaaagcta gtattgtaga atctttattg ttcggagcag 3180
tgcggcgcga ggcacatctg cgtttcagga acgcgaccgg tgaagaccag gacgcacgga 3240
ggagagtctt ccgtcggagg gctgtcgccc gctcggcggc ttctaatccg tacttcaata 3300
tagcaatgag cagttaagcg tattactgaa agttccaaag agaaggtttt tttaggctaa 3360
gataatgggg ctctttacat ttccacaaca tataagtaag attagatatg gatatgtata 3420
tggtggtatt gccatgtaat atgattatta aacttctttg cgtccatcca aaaaaaaagt 3480
aagaattttt gaaaattcaa tataaatggt tttaaccaat aaaacagtca tttctggatc 3540
gaaagtcaaa agtttatcat ctgcgcaatc gagctcatca ggaccttcat catctagtga 3600
ggaagatgat tcccgcgata ttgaaagctt ggataagaaa atacgtcctt tagaagaatt 3660
agaagcatta ttaagtagtg gaaatacaaa acaattgaag aacaaagagg tcgctgcctt 3720
ggttattcac ggtaagttac ctttgtacgc tttggagaaa aaattaggtg atactacgag 3780
agcggttgcg gtacgtagga aggctctttc aattttggca gaagctcctg tattagcatc 3840
tgatcgttta ccatataaaa attatgacta cgaccgcgta tttggcgctt gttgtgaaaa 3900
tgttataggt tacatgcctt tgcccgttgg tgttataggc cccttggtta tcgatggtac 3960
atcttatcat ataccaatgg caactacaga gggttgtttg gtagcttctg ccatgcgtgg 4020
ctgtaaggca atcaatgctg gcggtggtgc aacaactgtt ttaactaagg atggtatgac 4080
aagaggccca gtagtccgtt tcccaacttt gaaaagatct ggtgcctgta agatatggtt 4140
agactcagaa gagggacaaa acgcaattaa aaaagctttt aactctacat caagatttgc 4200
acgtctgcaa catattcaaa cttgtctagc aggagattta ctcttcatga gatttagaac 4260
aactactggt gacgcaatgg gtatgaatat gatttctaaa ggtgtcgaat actcattaaa 4320
gcaaatggta gaagagtatg gctgggaaga tatggaggtt gtctccgttt ctggtaacta 4380
ctgtaccgac aaaaaaccag ctgccatcaa ctggatcgaa ggtcgtggta agagtgtcgt 4440
cgcagaagct actattcctg gtgatgttgt cagaaaagtg ttaaaaagtg atgtttccgc 4500
attggttgag ttgaacattg ctaagaattt ggttggatct gcaatggctg ggtctgttgg 4560
tggatttaac gcacatgcag ctaatttagt gacagctgtt ttcttggcat taggacaaga 4620
tcctgcacaa aatgttgaaa gttccaactg tataacattg atgaaagaag tggacggtga 4680
tttgagaatt tccgtatcca tgccatccat cgaagtaggt accatcggtg gtggtactgt 4740
tctagaacca caaggtgcca tgttggactt attaggtgta agaggcccgc atgctaccgc 4800
tcctggtacc aacgcacgtc aattagcaag aatagttgcc tgtgccgtct tggcaggtga 4860
attatcctta tgtgctgccc tagcagccgg ccatttggtt caaagtcata tgacccacaa 4920
caggaaacct gctgaaccaa caaaacctaa caatttggac gccactgata taaatcgttt 4980
gaaagatggg tccgtcacct gcattaaatc ctaatttgcc agcttactat ccttcttgaa 5040
aatatgcact ctatatcttt tagttcttaa ttgcaacaca tagatttgct gtataacgaa 5100
ttttatgcta ttttttaaat ttggagttca gtgataaaag tgtcacagcg aatttcctca 5160
catgtaggga ccgaattgtt tacaagttct ctgtaccacc atggagacat caaagattga 5220
aaatctatgg aaagatatgg acggtagcaa caagaatata gcacgagccg cgaagttcat 5280
ttcgttactt ttgatatcgc tcacaactat tgcgaagcgc ttcagtgaaa aaatcataag 5340
gaaaagttgt aaatattatt ggtagtattc gtttggtaaa gtagaggggg taatttttcc 5400
cctttatttt gttcatacat tcttaaattg ctttgcctct ccttttggaa agctatactt 5460
cggagcactg ttgagcgaag gctcattaga tatattttct gtcattttcc ttaacccaaa 5520
aataagggaa agggtccaaa aagcgctcgg acaactgttg accgtgatcc gaaggactgg 5580
ctatacagtg ttcacaaaat agccaagctg aaaataatgt gtagctatgt tcagttagtt 5640
tggctagcaa agatataaaa gcaggtcgga aatatttatg ggcattatta tgcagagcat 5700
caacatgata aaaaaaaaca gttgaatatt ccctcaaaaa tgtcattacc gttcttaact 5760
tctgcaccgg gaaaggttat tatttttggt gaacactctg ctgtgtacaa caagcctgcc 5820
gtcgctgcta gtgtgtctgc gttgagaacc tacctgctaa taagcgagtc atctgcacca 5880
gatactattg aattggactt cccggacatt agctttaatc ataagtggtc catcaatgat 5940
ttcaatgcca tcaccgagga tcaagtaaac tcccaaaaat tggccaaggc tcaacaagcc 6000
accgatggct tgtctcagga actcgttagt cttttggatc cgttgttagc tcaactatcc 6060
gaatccttcc actaccatgc agcgttttgt ttcctgtata tgtttgtttg cctatgcccc 6120
catgccaaga atattaagtt ttctttaaag tctactttac ccatcggtgc tgggttgggc 6180
tcaagcgcct ctatttctgt atcactggcc ttagctatgg cctacttggg ggggttaata 6240
ggatctaatg acttggaaaa gctgtcagaa aacgataagc atatagtgaa tcaatgggcc 6300
ttcataggtg aaaagtgtat tcacggtacc ccttcaggaa tagataacgc tgtggccact 6360
tatggtaatg ccctgctatt tgaaaaagac tcacataatg gaacaataaa cacaaacaat 6420
tttaagttct tagatgattt cccagccatt ccaatgatcc taacctatac tagaattcca 6480
aggtctacaa aagatcttgt tgctcgcgtt cgtgtgttgg tcaccgagaa atttcctgaa 6540
gttatgaagc caattctaga tgccatgggt gaatgtgccc tacaaggctt agagatcatg 6600
actaagttaa gtaaatgtaa aggcaccgat gacgaggctg tagaaactaa taatgaactg 6660
tatgaacaac tattggaatt gataagaata aatcatggac tgcttgtctc aatcggtgtt 6720
tctcatcctg gattagaact tattaaaaat ctgagcgatg atttgagaat tggctccaca 6780
aaacttaccg gtgctggtgg cggcggttgc tctttgactt tgttacgaag agacattact 6840
caagagcaaa ttgacagctt caaaaagaaa ttgcaagatg attttagtta cgagacattt 6900
gaaacagact tgggtgggac tggctgctgt ttgttaagcg caaaaaattt gaataaagat 6960
cttaaaatca aatccctagt attccaatta tttgaaaata aaactaccac aaagcaacaa 7020
attgacgatc tattattgcc aggaaacacg aatttaccat ggacttcata agcgaatttc 7080
ttatgattta tgatttttat tattaaataa gttataaaaa aaataagtgt atacaaattt 7140
taaagtgact cttaggtttt aaaacgaaaa ttcttattct tgagtaactc tttcctgtag 7200
gtcaggttgc tttctcaggt atagcatgag gtcgctctta ttgaccacac ctctaccggt 7260
tacgtggatt gagccagcaa tacagatcat tattaaactg ttttgtacat gatgttagta 7320
tataatcgta aagcttttct aatatgtata ccttatacat ggaactccac agaacttgca 7380
aacataccaa aaatccttta ttcttgttca ctcattttac atcaaaaaat aatatttcag 7440
ttattaagga aaataaaaaa atagattaga gaagcatttt gaagaaatag tatattcttt 7500
tattgaacct aagagcgtga tatttttact cgaaataaaa tacgaaaaat ctatacactc 7560
atctttccga ctactattgg ctcctgctca aaaaaagagg gaaaaaaagc tccaaaattc 7620
tatcttttcc tatcgctcct gtcctatcct tattacgttc attactattt taatactatc 7680
cattctttta ttttcagtct aaaaaaaaca tttctcataa cgggaaaagc aaaaaaatgt 7740
caagcttata catcaaaaca ccactgcatg cattatctgc tggtccggat tctcatgcaa 7800
atagctccta ttatgacaat cttttattgc cttcattttc caatttatcc agtaatatca 7860
gcaggaacaa tatcacaacc gataacaata taaattcagc aagtcctagg aagtattcgt 7920
ttcattcgct caatgtgtcg ccaattcttt ccccaatatc tttggctaac gaaatacttg 7980
gaaaaaaatc aaatactgca ccagcatcgc cacatcatat ggattacaat ccaatttcct 8040
cattaacacc aggcaattca ccagaattca acaaggcaag cttatctcag attagtttta 8100
caaatccatt gaactatggg tctggcttag gtttttcctc taattcacaa cctcgactac 8160
cattgttaga caggctatcg tccgtctctt tatctaagag accggagcgc ccacaacaaa 8220
gcctaccatc actaaggcat ctgcaattat tacccagtcc tcttttacaa gaaaacgcag 8280
cacgcttccc tgatacatca aaacgtacat ctaattggaa aactgatctt acccattggt 8340
gtaaggatac aaactatcaa gactatgtta aaatacgcga agaagtggct catttcaagc 8400
cattaagtat acccaactta acgc 8424
<210> 19
<211> 7396
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 19
ggccgcgact caatgacttc gggatctaat tcagcataaa taatctcttc tccctcgcca 60
gcttcagcta caatcttgcc tctggggtcc accactatag agtggccata tgcatggtaa 120
gaactctgta gattccttgc gggagaacat aacatcacgt acacttggtt gtcaactgcc 180
ctgcttcttg ccagtaaatg ccaatgcaag ggccctgtga cagtgttgaa tgcactaggg 240
tagatcatgg caaacgcacc cttgcgtgca cttagcattg cgagttccgg aaacctcatg 300
tcatagcata tacctacgcc aaacttacca tatttcgtgt caatggtagt agacttctct 360
ccagggctca aggtttcact ttcatggaac gatataccgt taggaatgtc tacatcgaaa 420
agatggacct tccgatgctt gtcaatcaac ttgccatcct cgttgaaaat tatagaggtg 480
ttgtagattt tgtctgtctt tggatccaat tccggaattg ttcctcctac caagatgatt 540
ttaaacttat tggctaaatt agacagaaat tgaactgacg tcgaaggctc tttcggattg 600
ataacttctg aatactttct aaactgatca gtggagtacg gagaattgaa gcattctggc 660
aatacgacca acttggtatc cggctgttcc ttcatagccc tttcaatgaa cgttgcagcc 720
ctttgaagat tggccatttt gtcaggactc gagcctgaca gttggaccaa cgcaacttta 780
attttttgtg aaagaatctt cgaagcactc atactggcga tcttcacgcc ctcctgctat 840
tacaaaagct gtgtttttac aagaatcaaa ttaagttagc aagatatcat acaacattat 900
tgataatttc aatatcgtgt tcgtacctga tgacgtatct gtgcattgat aaggcccgca 960
tggtttcaga aagcagagcg gaacgattcc aaattagtgg ccttgtgctt tgcatgtcaa 1020
ttgtgttacc ttcagctcgt ggatttgttt tatcaataca cagtctacag tcaagnnnnn 1080
nnnnntatca aattttgcgt tcgagcgtat aaaatagccg ctgtagctac ttaagttcct 1140
gttcagccgg tagaggtgtg gtcaataaga gcgacctcat gctatacctg agaaagcaac 1200
ctgacctaca ggaaagagtt actcaagaat aagaattttc gttttaaaac ctaagagtca 1260
ctttaaaatt tgtatacact tatttttttt ataacttatt taataataaa aatcataaat 1320
cataagaaat tcgcttattc ctttggtaga ccagtctttg cgtcaatcaa agattcgttt 1380
gtttcttgtg ggcctgaacc gacttgagtt aaaatcactc tggcaacatc cttttgcaac 1440
tcaagatcca attcacgtgc agtaaagtta gatgattcaa attgatggtt gaaagcctca 1500
agctgctcag tagtaaattt cttgtcccat ccaggaacag agccaaacaa tttatagata 1560
aatgcaaaga gtttcgactc attttcagct aagtagtaca acacagcatt tggacctgca 1620
tcaaacgtgt atgcaacgat tgtttctccg taaaactgat taatggtgtg gcaccaactg 1680
atgatacgct tggaagtgtc attcatgtag aatattggag ggaaagagtc caaacatgtg 1740
gcatggaaag agttggaatc catcattgtt tcctttgcaa aggtggcgaa atctttttca 1800
acaatggctt tacgcatgac ttcaaatctc tttggtacga catgttcaat tctttcttta 1860
aatagttcgg aggttgccac ggtcaattgc ataccctgag tggaactcac atccttttta 1920
atatcgctga caactaggac acaagctttc atctgaggcc agtcagagct gtctgcgatt 1980
tgtactgcca tggaatcatg accatcttca gcttttccca tttcccaggc cacgtatccg 2040
ccaaacaacg atctacaagc tgaaccagac ccctttcttg ctattctaga tatttctgaa 2100
gttgactgtg gtaattggta taacttagca attgcagaga ccaatgcagc aaagccagca 2160
gcggaggaag ctaaaccagc tgctgtagga aagttatttt cggagacaat gtggagtttc 2220
cattgagata atgtgggcaa tgaggcgtcc ttcgattcca tttcctttct taattggcgt 2280
aggtcgcgca gacaattttg agttctttca ttgtcgatgc tgtgtggttc tccatttaac 2340
cacaaagtgt cgcgttcaaa ctcaggtgca gtagccgcag aggtcaacgt tctgaggtca 2400
tcttgcgata aagtcactga tatggacgaa ttggtgggca gattcaactt cgtgtccctt 2460
ttcccccaat acttaagggt tgcgatgttg acgggtgcgg taacggatgc tgtgtaaacg 2520
gtcatttttg agggaatatt caactgtttt tttttatcat gttgatgctc tgcataataa 2580
tgcccataaa tatttccgac ctgcttttat atctttgcta gccaaactaa ctgaacatag 2640
ctacacatta ttttcagctt ggctattttg tgaacactgt atagccagtc cttcggatca 2700
cggtcaacag ttgtccgagc gctttttgga ccctttccct tatttttggg ttaaggaaaa 2760
tgacagaaaa tatatctaat gagccttcgc tcaacagtgc tccgaagtat agctttccaa 2820
aaggagaggc aaagcaattt aagaatgtat gaacaaaata aaggggaaaa attaccccct 2880
ctactttacc aaacgaatac taccaataat atttacaact tttccttatg attttttcac 2940
tgaagcgctt cgcaatagtt gtgagcgata tcaaaagtaa cgaaatgaac ttcgcggctc 3000
gtgctatatt cttgttgcta ccgtccatat ctttccatag attttcaatc tttgatgtct 3060
ccatggtggt acagagaact tgtaaacaat tcggtcccta catgtgagga aattcgctgt 3120
gacactttta tcactgaact ccaaatttaa aaaatagcat aaaattcgtt atacagcaaa 3180
tctatgtgtt gcaattaaga actaaaagat atagagtgca tattttcaag aaggatagta 3240
agctggcaaa tcatatcttt tcaatgacaa tagaggaagc accaccacca ccattacaaa 3300
tggcggcaac accgatctta cctccttctt gctgtaagat ggatagcagt gtaacaacca 3360
ctctagcacc agaacaaccc aatgggtgac ctagagcaac agcaccacca tatacattaa 3420
ccttagatgg gtctagcttc aaaatcttag tgttcaccaa accgacaacc gaaaaggctt 3480
cattgaattc aaagtaatca acagaattga tgtcttcgat gccagcatgt ttcaaagcct 3540
ttggaactgc aagagatgga gcccatgtaa aatcagctgg ttgatgagcg gcctcacccc 3600
aacctttgat aatagccaaa ggcttcaaat tcttttcctt caaaactttt tcggaaacca 3660
agatgacggc tgcagcacca tcgttgattg gagaagcgtt agcggcagta acagtaccgt 3720
tttctttttg gaaaacagtc cttgcagatc tcaatttttc aacgtgtaat ctagcaggtt 3780
cctcgtcctt cgtgacttga gtatcaggct tacctctaaa tcccttaatg gtaacaggta 3840
caatttcatt gtcgaattta ccttcctttt gagatttttg agatttttgg taggattcga 3900
tggcaaaatt gtcttgttgt tctctagtaa tatcccaatc acgggcacac ttttctgcgt 3960
gtacacccat ggctagacca tcgtacgcat cgttcaaccc atctctttcg acaccatcaa 4020
caagaacagt ttggccaaat ttggcacccg cacgggctgc tggcatgtag tatggtgcgt 4080
tagtcataga ttcacaacca ccagctacga caacatcagc attaccacat ttgatggatt 4140
gagcacccaa aatgattgcc ttcatagcgg atgcacagac cttgttaact gtgcttgcaa 4200
cgatatgatt actcaaaccg gcagccaaag caacttgtct ggccggagct tggcccaaat 4260
tggcagaaag aacgttacca aaaataattt cgtcaaaatc cttggatgca tccaattctg 4320
gaaccttagc caaggcgcct tttaaagcaa cagcacccaa ttccactgct gtcttggagg 4380
atagagaacc ctggaatgaa ccaattgggg ttctggcagt cgatacaatg taaacgttct 4440
gagacattta tattgaattt tcaaaaattc ttactttttt tttggatgga cgcaaagaag 4500
tttaataatc atattacatg gcaataccac catatacata tccatatcta atcttactta 4560
tatgttgtgg aaatgtaaag agccccatta tcttagccta aaaaaacctt ctctttggaa 4620
ctttcagtaa tacgcttaac tgctcattgc tatattgaag tacggattag aagccgccga 4680
gcgggcgaca gccctccgac ggaagactct cctccgtgcg tcctggtctt caccggtcgc 4740
gttcctgaaa cgcagatgtg cctcgcgccg cactgctccg aacaataaag attctacaat 4800
actagctttt atggttatga agaggaaaaa ttggcagtaa cctggcccca caaaccttca 4860
aatcaacgaa tcaaattaac aaccatagga taataatgcg attagttttt tagccttatt 4920
tctggggtaa ttaatcagcg aagcgatgat ttttgatcta ttaacagata tataaatgca 4980
aaagctgcat aaccacttta actaatactt tcaacatttt cggtttgtat tacttcttat 5040
tcaaatgtca taaaagtatc aacaaaaaat tgttaatata cctctatact ttaacgtcaa 5100
ggagaaaaaa ctataatgac tgccgacaac aatagtatgc cccatggtgc agtatctagt 5160
tacgccaaat tagtgcaaaa ccaaacacct gaagacattt tggaagagtt tcctgaaatt 5220
attccattac aacaaagacc taatacccga tctagtgaga cgtcaaatga cgaaagcgga 5280
gaaacatgtt tttctggtca tgatgaggag caaattaagt taatgaatga aaattgtatt 5340
gttttggatt gggacgataa tgctattggt gccggtacca agaaagtttg tcatttaatg 5400
gaaaatattg aaaagggttt actacatcgt gcattctccg tctttatttt caatgaacaa 5460
ggtgaattac ttttacaaca aagagccact gaaaaaataa ctttccctga tctttggact 5520
aacacatgct gctctcatcc actatgtatt gatgacgaat taggtttgaa gggtaagcta 5580
gacgataaga ttaagggcgc tattactgcg gcggtgagaa aactagatca tgaattaggt 5640
attccagaag atgaaactaa gacaaggggt aagtttcact ttttaaacag aatccattac 5700
atggcaccaa gcaatgaacc atggggtgaa catgaaattg attacatcct attttataag 5760
atcaacgcta aagaaaactt gactgtcaac ccaaacgtca atgaagttag agacttcaaa 5820
tgggtttcac caaatgattt gaaaactatg tttgctgacc caagttacaa gtttacgcct 5880
tggtttaaga ttatttgcga gaattactta ttcaactggt gggagcaatt agatgacctt 5940
tctgaagtgg aaaatgacag gcaaattcat agaatgctat aaacaggccc cttttccttt 6000
gtcgatatca tgtaattagt tatgtcacgc ttacattcac gccctccccc cacatccgct 6060
ctaaccgaaa aggaaggagt tagacaacct gaagtctagg tccctattta tttttttata 6120
gttatgttag tattaagaac gttatttata tttcaaattt ttcttttttt tctgtacaaa 6180
cgcgtgtacg catgtaacat tatactgaaa accttgcttg agaaggtttt gggacgctcg 6240
aaggctttaa tttgcacgct gcaggtcgac aacccttaat ataggtgata tcagatccac 6300
tagtggcaat tccccattag agtcaaataa aaggatgcct cacggaggta tgttacccgc 6360
gctatttcac atggctcatt gaattagagg tggaatttgg tgtaccctcc cctcctcatc 6420
tgatgaagta gtgatccgac aattcttaaa agttgtagac attactttta ccaccaacta 6480
agttgtattt atattgctac ccttatcctt ttatatctaa ctagcgctca taaggttggg 6540
gcaatactaa aactgtgttc ttattcaact cattaaatac gtggcagtac gtaccctatt 6600
agaaacaata ggaaacagca gagtcggaag aagccaaatg ccagatttga agtccaaaac 6660
cttgtcaagc caatctttgg gagcggctat tcctccagaa attgtgtacc aaatacttac 6720
ataccagttt agggatttgt taagaaatga ccatccaggt acagcagaaa agtttaacga 6780
aaacttaact acatttgtca aaagtaattt aactgttaat aagacattct cccatatttg 6840
tcaagttctc atttatagat attgtaattt aactaccgca aagagatttc acggtctttt 6900
acagacttta aagggaaata gatgcctttg caataagatt gaagtagccg atttccaaga 6960
attaacgtct attggactgg ggaggtccag tgagatgaat aaaatgatta aaaatctaac 7020
taacgaaact cttcttgagt ttctaatgct cacaaaagca aacttaaggg aattcttagc 7080
atgtgaaaat attcaagacg atttagacga taatatcata aagtatattc tatctccaga 7140
aaaagtcttg agtgtagtgg atttctgtgg ctgttcaggt accactttca cagaaagttt 7200
tattaaggcc ctggataaat actaccctaa taaatcgatt gatcagtacc ggctggaacc 7260
tattcaacaa aattatcaga ttacctgctt aggcctaaat gattgtattg atctaccatc 7320
acacgttctt tggaagatct taaaaatgtt accagaacta caaaaacttg atttatctca 7380
tacctccatt gatggc 7396
<210> 20
<211> 6365
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 20
ggccgcctca aattacacac atacagatag gtcacatcat agaggtgata atgttttggg 60
tgatgcctct ttaggcgatt cgtttaattc aatgccagat tttactccaa gaaatttctt 120
gatacctaca ttaaaaaaat cacgggaaaa gttcgagcct catctatcca atccaaacca 180
acattttgga aatgtaacct tctaataatt tgtcatttga ctgacgaatc gttaggcaca 240
gttatatagt tatatacaca tagacataca tatgttcgca tatatattat attactatgc 300
attcacctaa atagctactg ctctattaat acagtgcttt tttgatggct ttaatttaac 360
ttattagaca ttactttgcg tttattcccc tccaatatat ttttatgttt gtttttcttg 420
aacattttat gatttcttgc tcttggcggc tactgatttt gttaagcaac tcatcaagag 480
gtcaaaaaaa gtagaacgag atctttgtgt tcggttaccc ggctcagatc ctaacttggt 540
atgtttattc gtataagtta ctgttgtcca caggcaatac tctgcagaaa attaaaacgg 600
cattaatgct aggacaacca gaattgttac tactgtatgt gcgatagttg ataactgcaa 660
cattatgccc ggtatattct caaaaaaccc tattactgca tacgaagaaa tcgcaagaga 720
aatctttcgg tttggaaaag ctcactgtga ggttccttgg agccaatagt aatacagcac 780
aatccaagga aaaatctggc ctatatgcaa ggaaggagag atagtcaaaa gcattctttc 840
ccctagaagt tggtgcatat atggcatcgt taaaacatat tacccccaaa atttcttctc 900
taaacgatgt gcttggcctt tgttttggtt tttgatgtcg gtcgtttgag gccccttgcg 960
gaaaatcgag atcgccgaat ggcacgcgag ggaagggaaa taaggtttaa aggcactgaa 1020
acaataggca agaagtaggc gagagccgac atacgagagc ttcattgctc ctacatttat 1080
cggatggcgc acatagctaa aaaaggaaga ttttcactca taagggaaag gaaccgcatg 1140
actcaagaga agcatgtggt ttttgagttt ttttcgttga attttcaggt aaagctcaat 1200
agttatgaca attacaacaa cagaattctt tctatatatg cacgaacttg taatatggaa 1260
gaaattatga cgtacaaact ataaagtaaa tattttacgt aacacatggt gctgttgtgc 1320
ttctttttca agagaatacc aatgacgtat gactaagttt aggatttaat gcaggtgacg 1380
gacccatctt tcaaacgatt tatatcagtg gcgtccaaat tgttaggttt tgttggttca 1440
gcaggtttcc tgttgtgggt catatgactt tgaaccaaat ggccggctgc tagggcagca 1500
cataaggata attcacctgc caagacggca caggcaacta ttcttgctaa ttgacgtgcg 1560
ttggtaccag gagcggtagc atgcgggcct cttacaccta ataagtccaa catggcacct 1620
tgtggttcta gaacagtacc accaccgatg gtacctactt cgatggatgg catggatacg 1680
gaaattctca aatcaccgtc cacttctttc atcaatgtta tacagttgga actttcaaca 1740
ttttgtgcag gatcttgtcc taatgccaag aaaacagctg tcactaaatt agctgcatgt 1800
gcgttaaatc caccaacaga cccagccatt gcagatccaa ccaaattctt agcaatgttc 1860
aactcaacca atgcggaaac atcacttttt aacacttttc tgacaacatc accaggaata 1920
gtagcttctg cgacgacact cttaccacga ccttcgatcc agttgatggc agctggtttt 1980
ttgtcggtac agtagttacc agaaacggag acaacctcca tatcttccca gccatactct 2040
tctaccattt gctttaatga gtattcgaca cctttagaaa tcatattcat acccattgcg 2100
tcaccagtag ttgttctaaa tctcatgaag agtaaatctc ctgctagaca agtttgaata 2160
tgttgcagac gtgcaaatct tgatgtagag ttaaaagctt ttttaattgc gttttgtccc 2220
tcttctgagt ctaaccatat cttacaggca ccagatcttt tcaaagttgg gaaacggact 2280
actgggcctc ttgtcatacc atccttagtt aaaacagttg ttgcaccacc gccagcattg 2340
attgccttac agccacgcat ggcagaagct accaaacaac cctctgtagt tgccattggt 2400
atatgataag atgtaccatc gataaccaag gggcctataa caccaacggg caaaggcatg 2460
taacctataa cattttcaca acaagcgcca aatacgcggt cgtagtcata atttttatat 2520
ggtaaacgat cagatgctaa tacaggagct tctgccaaaa ttgaaagagc cttcctacgt 2580
accgcaaccg ctctcgtagt atcacctaat tttttctcca aagcgtacaa aggtaactta 2640
ccgtgaataa ccaaggcagc gacctctttg ttcttcaatt gttttgtatt tccactactt 2700
aataatgctt ctaattcttc taaaggacgt attttcttat ccaagctttc aatatcgcgg 2760
gaatcatctt cctcactaga tgatgaaggt cctgatgagc tcgattgcgc agatgataaa 2820
cttttgactt tcgatccaga aatgactgtt ttattggtta aaaccattta tattgaattt 2880
tcaaaaattc ttactttttt tttggatgga cgcaaagaag tttaataatc atattacatg 2940
gcaataccac catatacata tccatatcta atcttactta tatgttgtgg aaatgtaaag 3000
agccccatta tcttagccta aaaaaacctt ctctttggaa ctttcagtaa tacgcttaac 3060
tgctcattgc tatattgaag tacggattag aagccgccga gcgggcgaca gccctccgac 3120
ggaagactct cctccgtgcg tcctggtctt caccggtcgc gttcctgaaa cgcagatgtg 3180
cctcgcgccg cactgctccg aacaataaag attctacaat actagctttt atggttatga 3240
agaggaaaaa ttggcagtaa cctggcccca caaaccttca aatcaacgaa tcaaattaac 3300
aaccatagga taataatgcg attagttttt tagccttatt tctggggtaa ttaatcagcg 3360
aagcgatgat ttttgatcta ttaacagata tataaatgca aaagctgcat aaccacttta 3420
actaatactt tcaacatttt cggtttgtat tacttcttat tcaaatgtca taaaagtatc 3480
aacaaaaaat tgttaatata cctctatact ttaacgtcaa ggagaaaaaa ctataatgaa 3540
actctcaact aaactttgtt ggtgtggtat taaaggaaga cttaggccgc aaaagcaaca 3600
acaattacac aatacaaact tgcaaatgac tgaactaaaa aaacaaaaga ccgctgaaca 3660
aaaaaccaga cctcaaaatg tcggtattaa aggtatccaa atttacatcc caactcaatg 3720
tgtcaaccaa tctgagctag agaaatttga tggcgtttct caaggtaaat acacaattgg 3780
tctgggccaa accaacatgt cttttgtcaa tgacagagaa gatatctact cgatgtccct 3840
aactgttttg tctaagttga tcaagagtta caacatcgac accaacaaaa ttggtagatt 3900
agaagtcggt actgaaactc tgattgacaa gtccaagtct gtcaagtctg tcttgatgca 3960
attgtttggt gaaaacactg acgtcgaagg tattgacacg cttaatgcct gttacggtgg 4020
taccaacgcg ttgttcaact ctttgaactg gattgaatct aacgcatggg atggtagaga 4080
cgccattgta gtttgcggtg atattgccat ctacgataag ggtgccgcaa gaccaaccgg 4140
tggtgccggt actgttgcta tgtggatcgg tcctgatgct ccaattgtat ttgactctgt 4200
aagagcttct tacatggaac acgcctacga tttttacaag ccagatttca ccagcgaata 4260
tccttacgtc gatggtcatt tttcattaac ttgttacgtc aaggctcttg atcaagttta 4320
caagagttat tccaagaagg ctatttctaa agggttggtt agcgatcccg ctggttcgga 4380
tgctttgaac gttttgaaat atttcgacta caacgttttc catgttccaa cctgtaaatt 4440
ggtcacaaaa tcatacggta gattactata taacgatttc agagccaatc ctcaattgtt 4500
cccagaagtt gacgccgaat tagctactcg cgattatgac gaatctttaa ccgataagaa 4560
cattgaaaaa acttttgtta atgttgctaa gccattccac aaagagagag ttgcccaatc 4620
tttgattgtt ccaacaaaca caggtaacat gtacaccgca tctgtttatg ccgcctttgc 4680
atctctatta aactatgttg gatctgacga cttacaaggc aagcgtgttg gtttattttc 4740
ttacggttcc ggtttagctg catctctata ttcttgcaaa attgttggtg acgtccaaca 4800
tattatcaag gaattagata ttactaacaa attagccaag agaatcaccg aaactccaaa 4860
ggattacgaa gctgccatcg aattgagaga aaatgcccat ttgaagaaga acttcaaacc 4920
tcaaggttcc attgagcatt tgcaaagtgg tgtttactac ttgaccaaca tcgatgacaa 4980
atttagaaga tcttacgatg ttaaaaaata atcatgtaat tagttatgtc acgcttacat 5040
tcacgccctc cccccacatc cgctctaacc gaaaaggaag gagttagaca acctgaagtc 5100
taggtcccta tttatttttt tatagttatg ttagtattaa gaacgttatt tatatttcaa 5160
atttttcttt tttttctgta cagacgcgtg tacgcatgta acattatact gaaaaccttg 5220
cttgagaagg ttttgggacg ctcgaaggct ttaatttgcc cacttttcaa tgaaacggat 5280
attgatatgc tagtaaaagg acgagctcaa gagcgaaaat ataagtaaag aattcgagtg 5340
cacttgtctc catgcagcaa gatttcatat gagtcttttt tatcttttta ctttttacat 5400
tacacgatat gcactttatg aaaatttaac gaggttggaa gccggataat caaccaaaat 5460
caggcacgaa ggcacactcg tatatgcatg ttgttgaaac tctgttacgc tgaactaaca 5520
atcacacatg tagaggtcac cgggaaaagt tgcgacccca tggaaggtcg atctcttcgt 5580
ttggctttgc ttggctggcg gcattgcgct tcttcgctta tacccgtctc ttgacgctcg 5640
agctcgttca ttgagatacc tttattcttg cacattttct ggcttttttc gctactcggg 5700
tacatgtaat catgcacaca gaaggtgctg tagggtgaaa gttcctttgt gctgtcgttt 5760
gtttttaatg ccaaactttc cggtgatcaa taaccacctc tttttccttc aggaaacctt 5820
attattgttc ttggatagta ctaggaagta tataaggaac ctcgattttg gtattgcacg 5880
gctatacaca tctaagaaac tttgtataaa aggtggctac cctattcata gcttgatatc 5940
aataggccat ctcatcactt tttattgaaa aggaaaggag ggaaatatat ctgattcaaa 6000
ttacttgttt gcttctcttt aagacaaaag catagataat ttcagcgtgg aacgccggaa 6060
taagattggt accctcgtca gaaagttaca aataccgctt catcttcaaa atgacttcac 6120
cggaatcact atcttctcgt catatcaggc aaggaaggac atacacaacc acagacaagg 6180
tcatatcgcg gtcgtcgtcg tactcatcta atagttcaat atctaaagat tacggcgatc 6240
acacaccctt gtccgtcagc agtgcagctt cagagacatt accctcacct cagtatatgc 6300
cgataaggac attcaataca atgcctacag ctggcccaac gcctttacat ttatttcaaa 6360
atggc 6365
<210> 21
<211> 58
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 21
gcttgagaag gttttgggac gctcgaaggc tttaatttgc ccgccatcca gtgtcgaa 58
<210> 22
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 22
cattatacga agttatatta agggttgtcg acctgcagcc ataacgcgtt acacggaag 59
<210> 23
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 23
cgggccctcc ttactgctct ccttccgtgt aacgcgttat ggctgcaggt cgacaaccc 59
<210> 24
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 24
agtaaaaaag gagtagaaac attttgaagc tatgagctcg ttagtatcga atcgacagc 59
<210> 25
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 25
ggtcgctata ctgctgtcga ttcgatacta acgagctcat agcttcaaaa tgtttctac 59
<210> 26
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 26
aagggttctc gagagctcgt tttcgacact ggatggcggg caaattaaag ccttcgagc 59
<210> 27
<211> 38
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 27
gggaacaaaa gcgcggccgc gacattgaac tcttggcc 38
<210> 28
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 28
tccaaaaaaa aagtaagaat ttttgaaaat tcaatataac ataacgcgtt acacggaag 59
<210> 29
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 29
ccttccgtgt aacgcgttat gttatattga attttcaaaa attcttactt tttttttgg 59
<210> 30
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 30
gtttagttga gagtttcatt atagtttttt ctccttgacg ttaa 44
<210> 31
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 31
caaggagaaa aaactataat gaaactctca actaaacttt gttg 44
<210> 32
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 32
gacataacta attacatgat tattttttaa catcgtaaga tcttcta 47
<210> 33
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 33
ttacgatgtt aaaaaataat catgtaatta gttatgtcac gcttac 46
<210> 34
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 34
attatacgaa gttatattaa gggttgtcga cctgcagcgc aaattaaagc cttcgagcg 59
<210> 35
<211> 58
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 35
gcttgagaag gttttgggac gctcgaaggc tttaatttgc gctgcaggtc gacaaccc 58
<210> 36
<211> 58
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 36
taagaaaaat ttcgagagag tcgccgatag tagccactag tggatctgat atcaccta 58
<210> 37
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 37
ctatacgaag ttattaggtg atatcagatc cactagtggc tactatcggc gactctctc 59
<210> 38
<211> 55
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 38
gagacggtca cagcttgtct gtgcggccgc ctgagcagca aaataccatt tttta 55
<210> 39
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 39
tggtattttg ctgctcaggc ggccgcacag acaagctgtg accgtc 46
<210> 40
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 40
ccaagagttc aatgtcgcgg ccgcgctttt gttcccttta gtgag 45
<210> 41
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 41
catgaggtcg ctcttattga ccacacctct accggttacg tggattgagc cagcaatac 59
<210> 42
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 42
aaggtggatg ggtaggttat atag 24
<210> 43
<211> 27
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 43
gtatctcttt gctatatatc tctgtgc 27
<210> 44
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 44
caatagtaat actatggtaa tgaatgacgc 30
<210> 45
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 45
cttcttcgta aatgtggccg ttat 24
<210> 46
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 46
cgtacaaagg taacttaccg tga 23
<210> 47
<211> 23
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 47
caattgaaga acaaagaggt cgc 23
<210> 48
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 48
gtttaataat gatctgtatt gctggctcaa tccacgtaac cggtagaggt gtggtcaat 59
<210> 49
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 49
gataaaggca tccccgatta tattc 25
<210> 50
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 50
cacaaagtat gcgcaatcc 19
<210> 51
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 51
taaatgatca tatgtctcta attttggaag ttttagagct agaaatagca agttaaaat 59
<210> 52
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 52
tttctagctc taaaacttcc aaaattagag acatatgatc atttatcttt cactgcgga 59
<210> 53
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 53
tcgacaaccc ttaatatagg tgatatcaga tcca 34
<210> 54
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 54
ctagtggatc tgatatcacc tatattaagg gttg 34
<210> 55
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 55
gataaaggca tccccgatta tattc 25
<210> 56
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 56
cacaaagtat gcgcaatcc 19
<210> 57
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 57
taaatgatcg cttcaagaat tgagtaaacg ttttagagct agaaatagca agttaaaat 59
<210> 58
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 58
tttctagctc taaaacgttt actcaattct tgaagcgatc atttatcttt cactgcgga 59
<210> 59
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 59
aaggttttgg gacgctcgaa ggctttaatt tgcccacttt tcaatgaaac ggatattga 59
<210> 60
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 60
gccattcagg ctgcgcaact gttgcggccg ccattttgaa ataaatgtaa aggcgttgg 59
<210> 61
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 61
gcccaacgcc tttacattta tttcaaaatg gcggccgcaa cagttgcgca gcctgaatg 59
<210> 62
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 62
cctatctgta tgtgtgtaat ttgaggcggc cgctactaga gctccagctt ttgttcc 57
<210> 63
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 63
ggaacaaaag ctggagctct agtagcggcc gcctcaaatt acacacatac agataggtc 59
<210> 64
<211> 58
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 64
ctatgtgcgc catccgataa atgtaggagc aatgaagctc tcgtatgtcg gctctcgc 58
<210> 65
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 65
taggcaagaa gtaggcgaga gccgacatac gagagcttca ttgctcctac atttatcgg 59
<210> 66
<211> 58
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 66
actagcatat caatatccgt ttcattgaaa agtgggcaaa ttaaagcctt cgagcgtc 58
<210> 67
<211> 25
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 67
gataaaggca tccccgatta tattc 25
<210> 68
<211> 19
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 68
cacaaagtat gcgcaatcc 19
<210> 69
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 69
taaatgatcc taatgtgtcc gcgtttctag ttttagagct agaaatagca agttaaaat 59
<210> 70
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 70
tttctagctc taaaactaga aacgcggaca cattaggatc atttatcttt cactgcgga 59
<210> 71
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 71
actaaaggga acaaaagctg gagctctagt agtttaaaca taacgagaac acacagggg 59
<210> 72
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 72
cattaaagta acttaaggag ttaaatttaa gcaaggattt tcttaacttc ttc 53
<210> 73
<211> 56
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 73
gaagttaaga aaatccttgc ttaaatttaa ctccttaagt tactttaatg atttag 56
<210> 74
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 74
tcgaaggctt taatttgcgc gaaaagccaa ttagtgtgat a 41
<210> 75
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 75
tagtatcaca ctaattggct tttcgcgcaa attaaagcct tcgagc 46
<210> 76
<211> 49
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 76
gggacgcgcc ctgtagcggc tgaggtctca acaggcccct tttcctttg 49
<210> 77
<211> 55
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 77
catgatatcg acaaaggaaa aggggcctgt tgagacctca gccgctacag ggcgc 55
<210> 78
<211> 52
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 78
gaatttttga aaattcaata taaatgtgag accaccatga ttacgccaag cg 52
<210> 79
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 79
taatcatggt ggtctcacat ttatattgaa ttttcaaaaa ttcttacttt ttttttg 57
<210> 80
<211> 62
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 80
atctctctct cctaatttct ttttctgaag ccattatagt tttttctcct tgacgttaaa 60
gt 62
<210> 81
<211> 50
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 81
ttaacgtcaa ggagaaaaaa ctataatggc ttcagaaaaa gaaattagga 50
<210> 82
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 82
atgtacaaat atcataaaaa aagagaatct ttttaaaaaa aatccttgga ctagtcacg 59
<210> 83
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 83
actagtccaa ggattttttt taaaaagatt ctcttttttt atgatatttg tacataaac 59
<210> 84
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 84
gcgccattcg ccattcaggc tgcgcaactg ttgtttaaac gacaacgacc aagctcaca 59
<210> 85
<211> 50
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 85
gatgtgagct tggtcgttgt cgtttaaaca acagttgcgc agcctgaatg 50
<210> 86
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 86
cgatagcgcc cctgtgtgtt ctcgttatgt ttaaactact agagctccag cttttgttc 59
<210> 87
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 87
aaaggtctca ctgttcaaat gaccattggg tggacaaag 39
<210> 88
<211> 58
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 88
aaaggtctca aatgtctact atccctgttt ccagtgtttc ttcctctagt tctgcctc 58
<210> 89
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 89
attaaccctc actaaaggga acaaaagcgt ttaaacacgc agataattcc aggtatttt 59
<210> 90
<211> 54
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 90
aatacgactc actatagggc gaattgggta ccttcgtttc ctgcaggttt ttgt 54
<210> 91
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 91
caaaaacctg caggaaacga aggtacccaa ttcgccctat agtgag 46
<210> 92
<211> 50
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 92
gttttgggac gctcgaaggc tttaatttgc tcacagcttg tctgtaagcg 50
<210> 93
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 93
ttgtctgctc ccggcatccg cttacagaca agctgtgagc aaattaaagc cttcgagcg 59
<210> 94
<211> 42
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 94
gtccacccaa tggtcatttg aacaggcccc ttttcctttg tc 42
<210> 95
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 95
taattacatg atatcgacaa aggaaaaggg gcctgttcaa atgaccattg ggtggac 57
<210> 96
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 96
gaatttttga aaattcaata taaatgtcta ctatccctgt ttcca 45
<210> 97
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 97
cagggatagt agacatttat attgaatttt caaaaattct tacttttttt ttg 53
<210> 98
<211> 49
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 98
ggaaacaggg atagtagaca ttatagtttt ttctccttga cgttaaagt 49
<210> 99
<211> 55
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 99
ctctatactt taacgtcaag gagaaaaaac tataatgtct actatccctg tttcc 55
<210> 100
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 100
aaattgatct atcgatttca attcaattca attcaaatga ccattgggtg gac 53
<210> 101
<211> 49
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 101
ccacccaatg gtcatttgaa ttgaattgaa ttgaaatcga tagatcaat 49
<210> 102
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 102
ttgaagctct aatttgtgag tttagtatac atgcatttac aacgaacgca gaattttcg 59
<210> 103
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 103
gtttaataac tcgaaaattc tgcgttcgtt gtaaatgcat gtatactaaa ctcacaaat 59
<210> 104
<211> 50
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 104
gacggtcaca gcttgtctgt gtttaaaccg tttaagggca aatgtactct 50
<210> 105
<211> 50
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 105
agagtacatt tgcccttaaa cggtttaaac acagacaagc tgtgaccgtc 50
<210> 106
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 106
tgcttcaaaa tacctggaat tatctgcgtg tttaaacgct tttgttccct ttagtgagg 59
<210> 107
<211> 48
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 107
ctaaagggaa caaaagcgcg gccgcggcaa tttggtacaa aaatcacg 48
<210> 108
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 108
ctatattata tatatagtaa tgtcgttttt gcgaaaccct atgctc 46
<210> 109
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 109
gagcataggg tttcgcaaaa acgacattac tatatatata atatag 46
<210> 110
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 110
gctcgaaggc tttaatttgc cctgatgcgg tattttctcc 40
<210> 111
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 111
ggagaaaata ccgcatcagg gcaaattaaa gccttcgagc g 41
<210> 112
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 112
ctttgtccac ccaatggtca tttgaacagg ccccttttcc tttgtc 46
<210> 113
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 113
atcgacaaag gaaaaggggc ctgttcaaat gaccattggg tggac 45
<210> 114
<211> 51
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 114
gtaagaattt ttgaaaattc aatataaatg tctactatcc ctgtttccag t 51
<210> 115
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 115
cagggatagt agacatttat attgaatttt caaaaattct tacttttttt ttg 53
<210> 116
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 116
cactggaaac agggatagta gacattatag ttttttctcc ttgacgttaa agt 53
<210> 117
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 117
cgtcaaggag aaaaaactat aatgtctact atccctgttt ccagtg 46
<210> 118
<211> 48
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 118
gatctatcga tttcaattca attcaattca aatgaccatt gggtggac 48
<210> 119
<211> 49
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 119
ccacccaatg gtcatttgaa ttgaattgaa ttgaaatcga tagatcaat 49
<210> 120
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 120
gctcatcccg accttccatt aacgaacgca gaattttcga g 41
<210> 121
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 121
ctcgaaaatt ctgcgttcgt taatggaagg tcgggatgag c 41
<210> 122
<211> 52
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 122
agacggtcac agcttgtctg tgcggccgcg cttctaataa accgatgaac gc 52
<210> 123
<211> 52
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 123
gcgttcatcg gtttattaga agcgcggccg cacagacaag ctgtgaccgt ct 52
<210> 124
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 124
ttttgtacca aattgccgcg gccgcgcttt tgttcccttt agtgagg 47
<210> 125
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 125
aaaggtctca gatctccaaa gtgacaggtg ccccgtttta gagctagaaa tagcaagtt 59
<210> 126
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 126
aaaggtctca aaactctaga ctttttcgat gatgtagttt c 41
<210> 127
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 127
gctcggtacc cggggatcct ctagagattg tttaaacttt cctctaatca ggttccacc 59
<210> 128
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 128
ggttttggga cgctcgaagg ctttaatttg ctcactttgg aaaaaaaata tacgctaag 59
<210> 129
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 129
tctccaaaaa tcttagcgta tatttttttt ccaaagtgag caaattaaag ccttcgagc 59
<210> 130
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 130
cgtacaggcg gtaagcactg aacaggcccc ttttccttt 39
<210> 131
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 131
caaaggaaaa ggggcctgtt cagtgcttac cgcctgtac 39
<210> 132
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 132
acgtcaagga gaaaaaacta taatgatcag acaatctacg ctaaa 45
<210> 133
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 133
gcgtagattg tctgatcatt atagtttttt ctccttgacg ttaaag 46
<210> 134
<211> 50
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 134
ggcttgagtt ctcaacattt atattgaatt ttcaaaaatt cttacttttt 50
<210> 135
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 135
ttttgaaaat tcaatataaa tgttgagaac tcaagccgcc 40
<210> 136
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 136
ggatagtaag ctggcaaatt attggttttc tggtctcaac 40
<210> 137
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 137
gaccagaaaa ccaataattt gccagcttac tatccttctt 40
<210> 138
<211> 48
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 138
caactttcgt taacaagccc atttttgagg gaatattcaa ctgttttt 48
<210> 139
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 139
aacagttgaa tattccctca aaaatgggct tgttaacgaa agttgc 46
<210> 140
<211> 50
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 140
aatcataaat cataagaaat tcgctcaagc atctaaaaca caaccgttgg 50
<210> 141
<211> 48
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 141
cggttgtgtt ttagatgctt gagcgaattt cttatgattt atgatttt 48
<210> 142
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 142
taatagaaaa agaaattgca accccggtag aggtgtggtc aataa 45
<210> 143
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 143
gaccacacct ctaccggggt tgcaatttct ttttctatta gta 43
<210> 144
<211> 56
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 144
caataacgat ctcagcatta tgaaagaatt atttttttta ttatgttaat cttgtg 56
<210> 145
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 145
ataaaaaaaa taattctttc ataatgctga gatcgttatt gca 43
<210> 146
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 146
tcgattagcg ttagttctaa ccaggtggta gttgggaaat gtcg 44
<210> 147
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 147
aactaccacc tggttagaac taacgctaat cgataaaaca t 41
<210> 148
<211> 56
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 148
gtgaggaact gggttacccg gggcacctgt taaaaaaaat ccttggacta gtcacg 56
<210> 149
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 149
caaggatttt ttttaacagg tgccccgggt aacc 34
<210> 150
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 150
ccaagcttgc atgcctgcag gtcgacgatg tttaaaccat gagagtagca aacgtaagt 59
<210> 151
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 151
gctcggtacc cggggatcct ctagagattg tttaaacttt cctctaatca ggttccacc 59
<210> 152
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 152
ggttttggga cgctcgaagg ctttaatttg ctcactttgg aaaaaaaata tacgctaag 59
<210> 153
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 153
tctccaaaaa tcttagcgta tatttttttt ccaaagtgag caaattaaag ccttcgagc 59
<210> 154
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 154
ctcttcaatt aacgaaaagt aaacaggccc cttttccttt 40
<210> 155
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 155
caaaggaaaa ggggcctgtt tacttttcgt taattgaaga gaggaat 47
<210> 156
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 156
caaggagaaa aaactataat gaatataatg aaaagacaat tatgcac 47
<210> 157
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 157
tcttttcatt atattcatta tagttttttc tccttgacgt taa 43
<210> 158
<211> 58
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 158
atagtacttc tgtgtggtgc agtcatttat attgaatttt caaaaattct tacttttt 58
<210> 159
<211> 52
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 159
taagaatttt tgaaaattca atataaatga ctgcaccaca cagaagtact at 52
<210> 160
<211> 52
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 160
ttttcaagaa ggatagtaag ctggcaaatc agtcctttct ccagtcacca tt 52
<210> 161
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 161
tgactggaga aaggactgat ttgccagctt actatccttc 40
<210> 162
<211> 50
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 162
tcgacagtat ggaagatttt catttttgag ggaatattca actgtttttt 50
<210> 163
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 163
gttgaatatt ccctcaaaaa tgaaaatctt ccatactgtc gaag 44
<210> 164
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 164
atcataaatc ataagaaatt cgcttaaata acgatgttat ctattagtct gac 53
<210> 165
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 165
tagataacat cgttatttaa gcgaatttct tatgatttat gatttt 46
<210> 166
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 166
taatagaaaa agaaattgca accccggtag aggtgtggtc aataa 45
<210> 167
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 167
gaccacacct ctaccggggt tgcaatttct ttttctatta gta 43
<210> 168
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 168
gcaacatggt tctaatcatt atgaaagaat tatttttttt attatgttaa tcttgtg 57
<210> 169
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 169
aaaaaataat tctttcataa tgattagaac catgttgcaa g 41
<210> 170
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 170
tttatcgatt agcgttagtt tcaagcaacc tggactggta tag 43
<210> 171
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 171
caggttgctt gaaactaacg ctaatcgata aaaca 35
<210> 172
<211> 56
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 172
gtgaggaact gggttacccg gggcacctgt taaaaaaaat ccttggacta gtcacg 56
<210> 173
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 173
caaggatttt ttttaacagg tgccccgggt aacc 34
<210> 174
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 174
ccaagcttgc atgcctgcag gtcgacgatg tttaaaccat gagagtagca aacgtaagt 59
<210> 175
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 175
gctcggtacc cggggatcct ctagagattg tttaaacttt cctctaatca ggttccacc 59
<210> 176
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 176
ggttttggga cgctcgaagg ctttaatttg ctcactttgg aaaaaaaata tacgctaag 59
<210> 177
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 177
tctccaaaaa tcttagcgta tatttttttt ccaaagtgag caaattaaag ccttcgagc 59
<210> 178
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 178
ctcttcaatt aacgaaaagt aaacaggccc cttttccttt 40
<210> 179
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 179
caaaggaaaa ggggcctgtt tacttttcgt taattgaaga gaggaat 47
<210> 180
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 180
caaggagaaa aaactataat gaatataatg aaaagacaat tatgcac 47
<210> 181
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 181
tcttttcatt atattcatta tagttttttc tccttgacgt taa 43
<210> 182
<211> 58
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 182
atagtacttc tgtgtggtgc agtcatttat attgaatttt caaaaattct tacttttt 58
<210> 183
<211> 52
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 183
taagaatttt tgaaaattca atataaatga ctgcaccaca cagaagtact at 52
<210> 184
<211> 52
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 184
ttttcaagaa ggatagtaag ctggcaaatc agtcctttct ccagtcacca tt 52
<210> 185
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 185
tgactggaga aaggactgat ttgccagctt actatccttc 40
<210> 186
<211> 50
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 186
tcgacagtat ggaagatttt catttttgag ggaatattca actgtttttt 50
<210> 187
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 187
gttgaatatt ccctcaaaaa tgaaaatctt ccatactgtc gaag 44
<210> 188
<211> 53
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 188
atcataaatc ataagaaatt cgcttaaata acgatgttat ctattagtct gac 53
<210> 189
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 189
tagataacat cgttatttaa gcgaatttct tatgatttat gatttt 46
<210> 190
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 190
aactgggtta cccggggcac ctgccggtag aggtgtggtc aataa 45
<210> 191
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 191
gaccacacct ctaccggcag gtgccccggg taacc 35
<210> 192
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 192
ccaagcttgc atgcctgcag gtcgacgatg tttaaaccat gagagtagca aacgtaagt 59
<210> 193
<211> 24
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 193
caatggtcta ggtagtggca ttcg 24
<210> 194
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 194
cgactcacta tagggcgaat tgggtacgac gggagtggaa agaacgg 47
<210> 195
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 195
tcccgttctt tccactcccg tcgtacccaa ttcgccctat agtgag 46
<210> 196
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 196
gccaagcaca gggcaagatg ctttcacagc ttgtctgtaa gcgga 45
<210> 197
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 197
gcatccgctt acagacaagc tgtgaaagca tcttgccctg tgctt 45
<210> 198
<211> 29
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 198
gagaccacca agaatacaga agctattat 29
<210> 199
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 199
gctcggtacc cggggatcct ctagagattg tttaaacttt cctctaatca ggttccacc 59
<210> 200
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 200
cagatgagac ctgcatcact ttggaaaaaa aatatacgc 39
<210> 201
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 201
atattttttt tccaaagtga tgcaggtctc atctggaata taattc 46
<210> 202
<211> 42
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 202
taatagcttt cccatgattt tacgtatatc aactagttga cg 42
<210> 203
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 203
caactagttg atatacgtaa aatcatggga aagctattac aattggc 47
<210> 204
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 204
gttttatcga ttagcgttag tttcacgctc tgtgtaaagt gtat 44
<210> 205
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 205
cactttacac agagcgtgaa actaacgcta atcgataaaa catt 44
<210> 206
<211> 56
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 206
gtgaggaact gggttacccg gggcacctgt taaaaaaaat ccttggacta gtcacg 56
<210> 207
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 207
caaggatttt ttttaacagg tgccccgggt aacc 34
<210> 208
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 208
ccaagcttgc atgcctgcag gtcgacgatg tttaaaccat gagagtagca aacgtaagt 59
<210> 209
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 209
cgagctcggt acccggggat cctctagaga ttgtttaaac tatcatccac tgcacccac 59
<210> 210
<211> 57
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 210
gctcggcggc ttctaatccg tttagttggt aattaactag aaaaagaaag taaacaa 57
<210> 211
<211> 38
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 211
ctagttaatt accaactaaa cggattagaa gccgccga 38
<210> 212
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 212
aatttaaccc tgacaaacat ctccttgacg ttaaagtata gagg 44
<210> 213
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 213
tatactttaa cgtcaaggag atgtttgtca gggttaaatt gaat 44
<210> 214
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 214
tctagaatcc ggggtttttt ttaatcatta tcagtctgtc tcttggt 47
<210> 215
<211> 42
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 215
gacagactga taatgattaa aaaaaacccc ggattctaga ac 42
<210> 216
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 216
atgagcggac tgagggcgac gcaaattaaa gccttcgagc 40
<210> 217
<211> 38
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 217
gctcgaaggc tttaatttgc gtcgccctca gtccgctc 38
<210> 218
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 218
gcttgcatgc ctgcaggtcg acgatgttta aacattaagc gtgatattca ataacttcc 59
<210> 219
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 219
aaaggtctca gatcatcaat ggtttgaacg gaaggtttta gagctagaaa tagcaagtt 59
<210> 220
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 220
aaaggtctca ttttcaatat gtgcgcaagc ccggaatcg 39
<210> 221
<211> 48
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 221
aaaggtctca aaaaaatact ctggttttag agctagaaat agcaagtt 48
<210> 222
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 222
aaaggtctca aaactctaga ctttttcgat gatgtagttt c 41
<210> 223
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 223
cccggggatc ctctagagat tgtttaaacc ttttttttat tgtttcctgt tgtttttct 59
<210> 224
<211> 52
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 224
gctcggcggc ttctaatccg gaataaaaaa gaatatatac tccacatgac at 52
<210> 225
<211> 38
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 225
gtatatattc ttttttattc cggattagaa gccgccga 38
<210> 226
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 226
tgagtttctg gaatagacat ctccttgacg ttaaagtata gagg 44
<210> 227
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 227
tatactttaa cgtcaaggag atgtctattc cagaaactca aaaagc 46
<210> 228
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 228
aaattggcag taacctggcc atgagaaata tcgagggaga cga 43
<210> 229
<211> 38
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 229
tctccctcga tatttctcat ggccaggtta ctgccaat 38
<210> 230
<211> 58
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 230
ttatgttcct tgattgtcat ttatattgaa ttttcaaaaa ttcttacttt ttttttgg 58
<210> 231
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 231
tttttgaaaa ttcaatataa atgacaatca aggaacataa agtagt 46
<210> 232
<211> 52
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 232
aaggatagta agctggcaaa ttatttcttt ttttgagaga aaaattggtt ct 52
<210> 233
<211> 42
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 233
tctctcaaaa aaagaaataa tttgccagct tactatcctt ct 42
<210> 234
<211> 55
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 234
agcagtgtca aagtgtagct tagtcatttt tgagggaata ttcaactgtt ttttt 55
<210> 235
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 235
agttgaatat tccctcaaaa atgactaagc tacactttga cac 43
<210> 236
<211> 50
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 236
tctagaatcc ggggtttttt ttacaactta attctgacag cttttacttc 50
<210> 237
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 237
ctgtcagaat taagttgtaa aaaaaacccc ggattctaga acta 44
<210> 238
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 238
aatcatagca aacgaccaaa gcaaattaaa gccttcgagc 40
<210> 239
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 239
gctcgaaggc tttaatttgc tttggtcgtt tgctatgatt taattct 47
<210> 240
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 240
gcaggtcgac gatgtttaaa ccgccgaggg tattttactt c 41
<210> 241
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 241
gatcctctag agattgttta aactcaggag aacttccctg tgcata 46
<210> 242
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 242
tattccagat gagacctgca attgttgtct ttttttgtct tttgt 45
<210> 243
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 243
agacaaaaaa agacaacaat tgcaggtctc atctggaata 40
<210> 244
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 244
tcaataacgt gtccgccatg attttacgta tatcaactag ttgacg 46
<210> 245
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 245
gttgatatac gtaaaatcat ggcggacacg ttattga 37
<210> 246
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 246
ttatcgatta gcgttagttt tatgagtcat gatttactaa aggctg 46
<210> 247
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 247
gtaaatcatg actcataaaa ctaacgctaa tcgataaaac 40
<210> 248
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 248
catggaaatg ttgatccatt aaaaaaaatc cttggactag tcacg 45
<210> 249
<211> 32
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 249
gatttttttt aatggatcaa catttccatg at 32
<210> 250
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 250
tgcaggtcga cgatgtttaa actatatata tatatatatg tatcactaag ctcctaatt 59
<210> 251
<211> 42
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 251
gatcctctag agattgttta aacctcggcc ttcgttcatc tc 42
<210> 252
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 252
tattccagat gagacctgca tttgatgcgt acttcttagt tttt 44
<210> 253
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 253
agaagtacgc atcaaatgca ggtctcatct ggaatat 37
<210> 254
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 254
gaataagctt tcttcgctca tgattttacg tatatcaact agttg 45
<210> 255
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 255
gatatacgta aaatcatgag cgaagaaagc ttattcg 37
<210> 256
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 256
cgattagcgt tagttttatt tcaaagtctt caacaatttt tct 43
<210> 257
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 257
gaagactttg aaataaaact aacgctaatc gataaaacat tag 43
<210> 258
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 258
gtaaaaaact atgtcttcct ttaaaaaaaa tccttggact agtcacg 47
<210> 259
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 259
aggatttttt ttaaaggaag acatagtttt ttactttccc 40
<210> 260
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 260
ctgcaggtcg acgatgttta aacagtcata aattcaaata tcccgtt 47
<210> 261
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 261
gatcctctag agattgcggc cgcgtcaaag cactaataca ttgggg 46
<210> 262
<211> 52
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 262
tttatccatt ggaccgtgta atcgcaatac taatttatta tattttcttt tt 52
<210> 263
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 263
taaattagta ttgcgattac acggtccaat ggataaa 37
<210> 264
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 264
ccgtttctac taagaaatag tctctgcttt tgtgcgcgt 39
<210> 265
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 265
gcgcacaaaa gcagagacta tttcttagta gaaacggcga c 41
<210> 266
<211> 30
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 266
aaatcctcga gatgaagccg gaagttgagc 30
<210> 267
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 267
cgtaaaatcc ctaggatgga cgcttactcc acaag 35
<210> 268
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 268
gatttcaatt caattcaatt taggattgtt catacttttc ccag 44
<210> 269
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 269
aacaatccta aattgaattg aattgaaatc gatagatca 39
<210> 270
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 270
gaaaacagga aacctctaca acgaacgcag aattttcgag 40
<210> 271
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 271
gaaaattctg cgttcgttgt agaggtttcc tgttttcct 39
<210> 272
<211> 52
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 272
gcctgcaggt cgacgatgcg gccgctggta aatgtagtca tgtcattgat tc 52
<210> 273
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 273
catttaccag cggccgcatc gtcgacctgc aggca 35
<210> 274
<211> 38
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 274
gtgctttgac gcggccgcaa tctctagagg atccccgg 38
<210> 275
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 275
aaaggtctca gatccacatt tttagagtca tcccgtttta gagctagaaa tagcaagtt 59
<210> 276
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 276
aaaggtctca cctgtcctca gatgcgcaag cccggaatcg aaccggg 47
<210> 277
<211> 48
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 277
aaaggtctca caggaggcaa aggttttaga gctagaaata gcaagtta 48
<210> 278
<211> 47
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 278
aaaggtctca tcttcaggat tctgcgcaag cccggaatcg aaccggg 47
<210> 279
<211> 48
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 279
aaaggtctca aagaaactgc tggttttaga gctagaaata gcaagtta 48
<210> 280
<211> 41
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 280
aaaggtctca aaactctaga ctttttcgat gatgtagttt c 41
<210> 281
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 281
gctcggtacc cggggatcct ctagagattg tttaaacttt cctctaatca ggttccacc 59
<210> 282
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 282
ggttttggga cgctcgaagg ctttaatttg ctcactttgg aaaaaaaata tacgctaag 59
<210> 283
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 283
tctccaaaaa tcttagcgta tatttttttt ccaaagtgag caaattaaag ccttcgagc 59
<210> 284
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 284
acatcgacgc tggcaatata aacaggcccc ttttccttt 39
<210> 285
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 285
gaaaaggggc ctgtttatat tgccagcgtc gatg 34
<210> 286
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 286
aacgtcaagg agaaaaaact ataatgacca tagataacta cgacaa 46
<210> 287
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 287
agttatctat ggtcattata gttttttctc cttgacgtta 40
<210> 288
<211> 51
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 288
acagtggtgt caaagacatt tatattgaat tttcaaaaat tcttactttt t 51
<210> 289
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 289
aaattcaata taaatgtctt tgacaccact gtgtg 35
<210> 290
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 290
agaaggatag taagctggca aattagtcat cggccagtct cgcac 45
<210> 291
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 291
cgagactggc cgatgactaa tttgccagct tactatcctt cttgaa 46
<210> 292
<211> 42
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 292
tattagagtc ctccattttt gagggaatat tcaactgttt tt 42
<210> 293
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 293
tattccctca aaaatggagg actctaataa tgaagcc 37
<210> 294
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 294
tcataagaaa ttcgcctaag ctttgtcgtc actattatc 39
<210> 295
<211> 43
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 295
gtgacgacaa agcttaggcg aatttcttat gatttatgat ttt 43
<210> 296
<211> 33
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 296
cccggggcac ctgccggtag aggtgtggtc aat 33
<210> 297
<211> 34
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 297
accacacctc taccggcagg tgccccgggt aacc 34
<210> 298
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 298
ccaagcttgc atgcctgcag gtcgacgatg tttaaaccat gagagtagca aacgtaagt 59
<210> 299
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 299
gctcggtacc cggggatcct ctagagattg tttaaacttt cctctaatca ggttccacc 59
<210> 300
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 300
ggttttggga cgctcgaagg ctttaatttg ctcactttgg aaaaaaaata tacgctaag 59
<210> 301
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 301
tctccaaaaa tcttagcgta tatttttttt ccaaagtgag caaattaaag ccttcgagc 59
<210> 302
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 302
cccaatattt gaagaaataa acaggcccct tttcctttgt 40
<210> 303
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 303
aggaaaaggg gcctgtttat ttcttcaaat attgggcca 39
<210> 304
<211> 42
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 304
gtcaaggaga aaaaactata atgtttacac caattgatca ag 42
<210> 305
<211> 44
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 305
tgatcaattg gtgtaaacat tatagttttt tctccttgac gtta 44
<210> 306
<211> 48
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 306
atcctttaac tgccatttat attgaatttt caaaaattct tacttttt 48
<210> 307
<211> 51
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 307
aaagtaagaa tttttgaaaa ttcaatataa atggcagtta aaggattagg c 51
<210> 308
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 308
atagtaagct ggcaaatcaa aaagcatttt taccgaact 39
<210> 309
<211> 45
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 309
cggtaaaaat gctttttgat ttgccagctt actatccttc ttgaa 45
<210> 310
<211> 39
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 310
taccagtaaa catttttgag ggaatattca actgttttt 39
<210> 311
<211> 37
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 311
ccctcaaaaa tgtttactgg tattgtagaa tgcatgg 37
<210> 312
<211> 46
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 312
atcataagaa attcgcttat ttatttaggt agtttctaac cttctc 46
<210> 313
<211> 48
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 313
gttagaaact acctaaataa ataagcgaat ttcttatgat ttatgatt 48
<210> 314
<211> 40
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 314
gttacccggg gcacctgccg gtagaggtgt ggtcaataag 40
<210> 315
<211> 35
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 315
gaccacacct ctaccggcag gtgccccggg taacc 35
<210> 316
<211> 59
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 316
ccaagcttgc atgcctgcag gtcgacgatg tttaaaccat gagagtagca aacgtaagt 59
<210> 317
<211> 58
<212> DNA
<213> 人工序列(Artificial Sequence)
<400> 317
aaaggtctca aatgtctact atccctgttt ccagtgtttc tttctctagt tctgcctc 58
Claims (10)
1.一种同时生产倍半萜和初级代谢产物的菌株，其特征在于，所述菌株含有甲羟戊酸途径的相关基因、部分或全部经过密码子优化的倍半萜的合成基因和初级代谢产物合成的相关基因；所述初级代谢产物包括泛酸、角鲨烯、脂肪酸和维生素B2中的至少一种；所述倍半萜包括β-法尼烯；
所述的甲羟戊酸途径的相关基因包括ERG10、ERG13、tHMG1、ERG12、ERG8、MVD1、IDI1和ERG20基因；所述甲羟戊酸途径中基因的拷贝数为ERG10：ERG13：tHMG1：ERG12：ERG8：MVD1：IDI1：ERG20＝2：2：X：2：2：2：2：2，X为≥1的整数；
所述β-法尼烯合成基因包括bFS，所述的bFS基因含有F11S、M35T、T319S、I434T、I460V突变，其核苷酸序列如SEQ ID NO.1所示。
2.根据权利要求1所述的同时生产倍半萜和初级代谢产物的菌株，其特征在于，所述泛酸合成的相关基因包括ilv2、ilv6、ilv5、ilv3、Ecm31p、pan5、pan6和PAND基因；所述的PAND基因经过密码子优化，其核苷酸序列如SEQ ID NO.2所示。
3.根据权利要求1或2所述的同时生产倍半萜和初级代谢产物的菌株，其特征在于，所述同时生产倍半萜和所述泛酸的菌株包括：
将质粒pPT1、pPT2和pXZ162共转化至酿酒酵母JVA139获得的菌株JFS1；
或者将所述菌株JFS1经过GAL80基因的敲除，获得的菌株JFS2；
或者将质粒pPT1、pPT3和pXZ162共转化至酿酒酵母JVA139获得的菌株JFS3；
或者将所述菌株JFS3经过GAL80基因的敲除，获得的菌株JFS4；
其中，
所述质粒pPT1为以pMD19T为骨架载体，启动子为pGAL1、pGAL2、pGAL7和pGAL10，包含ilv2、ilv6、ilv5和ilv3基因，所述质粒pPT1的核苷酸序列如SEQ ID NO.3所示；
所述质粒pPT2为以pMD19T为骨架载体，启动子为pGAL1、pGAL2、pGAL7和pGAL10，包含Ecm31p、pan5、pan6和PAND基因，所述质粒pPT2的核苷酸序列如SEQ ID NO.4所示；
所述质粒pPT3为以pMD19T为骨架载体、启动子为pGAL1、pGAL7、pGAL10、包含Ecm31p、pan5和pan6基因，所述质粒pPT3的核苷酸序列如SEQ ID NO.5所示；
所述质粒pXZ162的核苷酸序列如SEQ ID NO.6所示。
4.根据权利要求1所述的同时生产倍半萜和初级代谢产物的菌株，其特征在于，所述角鲨烯合成的相关基因包括POS5、ADH2、ACS2、ALD6和ERG9基因。
5.根据权利要求1或4所述的同时生产倍半萜和初级代谢产物的菌株，其特征在于，所述同时生产倍半萜和所述角鲨烯的菌株包括：
将质粒pXZ171、pXZ159线性化后和pXZ161共转化至酿酒酵母JVA139获得的菌株JSX1；后将质粒pSX01线性化后和pXZ162共转化至所述菌株JSX1获得的菌株JSX2；
或者将所述菌株JSX2经过GAL80基因的敲除，获得的菌株JSX3；
其中，
所述质粒pXZ171以pMD19T为骨架载体，启动子为pGAL1、pGAL7和pGAL10，包含ADH2、ACS2和ALD6基因，所述质粒pXZ171的核苷酸序列如SEQ ID NO.7所示；
所述质粒pXZ159以pMD19T为骨架载体，启动子为pGAL1，包含POS5基因，所述质粒pXZ159的核苷酸序列如SEQ ID NO.8所示；
所述质粒pSX01以pMD19T为骨架载体，启动子为pHXT1，包含ERG9基因，所述质粒pSX01的核苷酸序列如SEQ ID NO.9所示；
所述质粒pXZ161的核苷酸序列如SEQ ID NO.10所示。
6.根据权利要求1所述的同时生产倍半萜和初级代谢产物的菌株，其特征在于，所述脂肪酸合成的相关基因包括ACC1、FAS1和FAS2基因，并采用葡萄糖诱导型启动子pHXT1和大肠杆菌来源的截短信号肽的硫解酶tesA。
7.根据权利要求1或6所述的同时生产倍半萜和初级代谢产物的菌株，其特征在于，所述同时生产倍半萜和所述脂肪酸的菌株包括：
将质粒pZF01、pZF02和pZF03分别线性化后和pZF04共转化至酿酒酵母JVA139获得的菌株JZF01；
或者将所述菌株JZF01经过GAL80基因的敲除，获得的菌株JZF02；
其中，
所述质粒pZF01以pMD19T为骨架载体，启动子为pHXT1，包含tesA基因，所述质粒pZF01的核苷酸序列如SEQ ID NO.11所示；
所述质粒pZF02以pMD19T为骨架载体，启动子为pHXT1，包含ACC1基因，所述质粒pZF02的核苷酸序列如SEQ ID NO.12所示；
所述质粒pZF03以pMD19T为骨架载体，启动子为pHXT1，包含FAS1和FAS2基因，所述质粒pZF03的核苷酸序列如SEQ ID NO.13所示；
所述质粒pZF04的核苷酸序列如SEQ ID NO.14所示。
8.根据权利要求1所述的同时生产倍半萜和初级代谢产物的菌株，其特征在于，所述维生素B2合成的相关基因包括RIB1、RIB7、RIB2、RIB3、RIB4和RIB5基因。
9.根据权利要求1或8所述的同时生产倍半萜和初级代谢产物的菌株，其特征在于，所述同时生产倍半萜和所述维生素B2的菌株包括：
将质粒pVB1和pVB2分别线性化后和pXZ162共转化至酿酒酵母JVA139获得的菌株JVB01；
或者将所述菌株JVB01经过GAL80基因的敲除，获得的菌株JVB02；
其中，
所述质粒pVB1以pMD19T为骨架载体，启动子为pGAL1、pGAL7和pGAL10，包含RIB1、RIB7和RIB2基因，所述质粒pVB1的核苷酸序列如SEQ ID NO.15所示；
所述质粒pVB2以pMD19T为骨架载体，启动子为pGAL1、pGAL7和pGAL10，包含RIB3、RIB4和RIB5基因，所述质粒pVB2的核苷酸序列如SEQ ID NO.16所示；
所述质粒pXZ162的核苷酸序列如SEQ ID NO.6所示。
10.一种权利要求1-9任一项所述的同时生产倍半萜和初级代谢产物的菌在生产倍半萜和初级代谢产物中的应用。
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