CN115803428A - 一种利用干细胞规模化培养装置高效制备外泌体的方法 - Google Patents

一种利用干细胞规模化培养装置高效制备外泌体的方法 Download PDF

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CN115803428A
CN115803428A CN202180046761.9A CN202180046761A CN115803428A CN 115803428 A CN115803428 A CN 115803428A CN 202180046761 A CN202180046761 A CN 202180046761A CN 115803428 A CN115803428 A CN 115803428A
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culture
reaction kettle
pipeline
cells
stem cell
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陈海佳
陈东煌
李学家
崔梓豪
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Guangdong Guoke Cell Technology Co ltd
Guangzhou Sailaila Biological Gene Engineering Co ltd
Guangzhou Saliai StemCell Science and Technology Co Ltd
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Guangdong Guoke Cell Technology Co ltd
Guangzhou Sailaila Biological Gene Engineering Co ltd
Guangzhou Saliai StemCell Science and Technology Co Ltd
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Publication of CN115803428A publication Critical patent/CN115803428A/zh
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    • C—CHEMISTRY; METALLURGY
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    • C12M1/00—Apparatus for enzymology or microbiology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/04—Apparatus for enzymology or microbiology with gas introduction means
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    • C12M1/00—Apparatus for enzymology or microbiology
    • C12M1/36—Apparatus for enzymology or microbiology including condition or time responsive control, e.g. automatically controlled fermentors
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    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues

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Abstract

本申请提供了一种利用干细胞规模化培养装置高效制备外泌体的方法。本申请外泌体的制备采用干细胞规模化培养装置进行，该干细胞规模化培养装置包括反应釜、培养微环境调控系统、实时观察拍照系统、气体输送装置、细胞瓶与储液瓶。该方法与常规培养箱二维培养相比外泌体的产量较高，从而提高外泌体制备的效率，并且可同时扩展应用于其他组织来源的间充质干细胞及其他细胞的外泌体制备。

Description

PCT国内申请，说明书已公开。

Claims (10)

  1. PCT国内申请，权利要求书已公开。
CN202180046761.9A 2021-05-18 2021-05-18 一种利用干细胞规模化培养装置高效制备外泌体的方法 Pending CN115803428A (zh)

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PCT/CN2021/094292 WO2022241633A1 (zh) 2021-05-18 2021-05-18 一种利用干细胞规模化培养装置高效制备外泌体的方法

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CN115803428A true CN115803428A (zh) 2023-03-14

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CN115786107A (zh) * 2022-12-14 2023-03-14 深圳市寰宇生物科技有限公司 一种用于外泌体的高效制备装置及其方法

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CN102061260B (zh) * 2010-12-01 2013-03-20 汪华 一种医用细胞自动化生产装置及其生产方法
EP3137590A1 (en) * 2014-04-28 2017-03-08 VivaBioCell SpA Automated cell culturing and harvesting device
CN205874455U (zh) * 2016-08-03 2017-01-11 苏州大学附属第二医院 细胞间接共培养装置
CN111363680B (zh) * 2018-12-26 2023-10-13 浙江大学 一种用于外泌体分泌、分离和收集的脉冲搅拌式生物反应器
CN109468274A (zh) * 2018-12-29 2019-03-15 深圳市旷逸生物科技有限公司 一种利用细胞工厂制备临床级脐带间充质干细胞的工艺

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