Background
The disposable sanitary articles generally directly contact with human bodies and are disposable articles, including masks, tissues, sanitary articles for women in menstrual periods and the like, generally have strict disinfection standards on the market of the articles, and ensure the safety of normal use.
Most of the existing disinfection modes for disposable sanitary products adopt ethylene oxide and ionizing radiation disinfection methods to carry out sanitary disinfection and sterilization treatment on packaged and completely sealed finished products, and the packaged and completely sealed finished products reach the national standard of disposable sanitary products.
The disinfection principle of the ethylene oxide disinfection method is as follows: the ethylene oxide can kill most pathogenic microorganisms including bacterial propagules, viruses and fungi, and remove the pathogenic microorganisms attached to the sanitary products; the penetration force to the sterilized articles is strong, the sterilized articles can reach the deep part of the articles, and cannot be damaged in penetration, but ethylene oxide sterilization has certain residual property, and the residual quantity of ethylene oxide can be enabled to be less than or equal to 250 microgram/g generally by long-time analysis (basically more than or equal to 14 days), so that the safety standard is reached;
the principle of ionizing radiation disinfection is a method of using ionizing radiation to kill pathogens (including viruses) so as to eliminate the toxicity of the pathogens, and the bacterial proteins are subjected to photolysis and denaturation mainly through the sterilization effect of ultraviolet rays, so that bacteria die.
Relatively speaking, the disinfection treatment by adopting the disinfectant is more economical and applicable, generally, most of the traditional disinfectants adopt hydrogen peroxide as a main disinfection component, which can play a good disinfection and killing role on most bacteria, but hydrogen peroxide can not inactivate hydrophilic viruses (poliovirus) without lipid envelopes, so that the disinfection of practical disposable articles has certain defects, and the hydrogen peroxide with higher concentration can bring certain damage to the products, can cause fading and oxidation of part of the products, and has poor disinfection effect when the concentration is low, thus the disinfection can not be widely applied to disinfection of disposable articles.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a disinfection method of disposable sanitary products, which effectively ensures the disinfection efficiency and disinfection effect of the sanitary products, reduces the analysis time after disinfection and improves the overall production efficiency of the disposable sanitary products.
In order to achieve the purpose, the technical scheme of the invention is realized by the following technical scheme:
a method of sterilizing a disposable hygiene article, the method comprising the steps of:
(1) Raw material disinfection: the raw materials of the disposable sanitary article are primarily disinfected before processing;
(2) And (3) purifying a workshop: processing the disposable sanitary articles by using a dust-free workshop, and regularly disinfecting the workshop;
(3) Direct disinfection of finished products: directly spraying disinfectant to sterilize after the product is formed;
(4) Stacking and analyzing: preheating the sterilized products by hot air, directly stacking and packaging the products, and then conveying the products to a warehouse for analysis, namely finishing the whole sterilization;
preferably, the disinfectant is prepared by directly mixing a plurality of components, wherein the first component contains benzalkonium chloride as an active ingredient, the second component contains hydrogen peroxide as an active ingredient, and the third component contains diacetin.
Preferably, the raw material disinfection process adopts an ultraviolet lamp to carry out dry irradiation disinfection, and the disinfection time is 6-10h.
Preferably, the dust-free workshop is sterilized at regular time by spraying a disinfectant and irradiating the disinfectant with ultraviolet light for 2 to 4 hours.
Preferably, the dust-free plant is sterilized at regular intervals of 4 to 7 days.
Preferably, the disinfectant is mixed by the following components in percentage by mass: 48-49% of the first component, 48-49% of the second component and 2-4% of the third component, wherein the mixing mode is that the second component and the third component are mixed for 3-5min, then the first component is added, the first component is uniformly stirred by a nonmetal stirring rod, and when the disinfectant is used, the disinfectant and purified water are mixed and diluted according to the proportion of 1: 1-4.
Preferably, the concentration of benzalkonium chloride in the first component of the disinfectant is 3.2-13.2%, the content of hydrogen peroxide in the second component is 77.6-79.5 g/L, and the content of diacetin in the third component is 99.8%.
Preferably, the disinfectant is sprayed in an atomizing mode, and the size of atomized particles is 10-15 microns.
Preferably, the preheating temperature of hot air after the product is sterilized is 45-55 ℃, and the preheating time is 5-8min.
Preferably, the time for resolution after disinfection is 7-9h.
Preferably, the disinfection method is used for disinfecting disposable masks, paper towels and sanitary products for women during menstrual period.
The invention provides a method for disinfecting disposable sanitary articles, which has the advantages compared with the prior art that:
(1) According to the invention, raw materials and a processing workshop of the disposable sanitary product are disinfected, so that the disposable sanitary product is ensured to contact and breed bacteria as little as possible in the processing process, the subsequent disinfection efficiency is improved, and the safety of the whole processing process is ensured;
(2) The benzalkonium chloride, the hydrogen peroxide and the diacetin are mixed to prepare the disinfectant, so that staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, aspergillus niger and poliomyelitis virus can be effectively killed, the overall disinfection efficiency is improved, meanwhile, the volatilization of the disinfectant can be accelerated while the disinfection efficiency is improved through atomization disinfection and hot air preheating, the residue is reduced, the resolution time after disinfection is further reduced, and the overall processing efficiency of the disposable hygienic product is improved;
(3) The invention has the advantages of economic construction cost of production environment, convenient maintenance mode and low overall disinfection time, greatly reduces time cost and improves production efficiency.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention are clearly and completely described below in conjunction with the embodiments of the present invention, and it is obvious that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
preparing a disinfectant:
(1) Benzalkonium chloride with the concentration of 5% is set as a first component, a solution with the hydrogen peroxide content of 78.5g/L is set as a second component, and diacetin with the purity of 99.8% is set as a third component;
(2) Preparing the following components in percentage by weight: 48% of first component, 49% of second component and 3% of third component;
(3) Mixing according to the mass percentage: mixing the second component and the third component uniformly for 5min, adding the first component, and stirring uniformly by using a glass rod to obtain a disinfectant;
example 2:
taking a sanitary pad as an example:
(1) Diluting a disinfectant: the disinfectant prepared in the above example 1 is diluted by adding 2 times of purified water, and the disinfectant prepared in the example 1 is used within 8h of mixing;
(2) Before the sanitary pad is processed in a factory, the raw materials are placed in a disinfection chamber, and an ultraviolet lamp is adopted for dry irradiation disinfection for 8 hours;
(3) Selecting a dust-free workshop as a workshop for processing the sanitary pad, spraying diluted disinfectant to the workshop once every 7d, irradiating for 2 hours by using ultraviolet light after spraying is finished, and producing the sanitary pad on a full-servo production line;
(3) Processing the disinfected sanitary pad raw materials in the disinfected dust-free workshop, arranging a disinfection spray gun at the outlet of a finished product, atomizing the diluted disinfectant into particles with the size of 10-15 mu m to disinfect the sanitary pad, wherein the dosage of the diluted disinfectant sprayed on each sanitary pad is 0.1-0.2g;
(4) The sanitary pad sprayed with the disinfectant is preheated for 6min by adopting hot air at 50 ℃, then the machine table folds and packages the product, the product is transported to a warehouse for analysis and disinfection for 8h after being packaged, and then the whole disinfection treatment is completed, and the product can be transported out of a factory.
Comparative example 1:
taking a sanitary pad as an example:
selecting the same raw materials as in example 1, processing in a common dust-free workshop, folding and maintaining the standards after the processing is finished, then feeding the raw materials into an ethylene oxide disinfection cabinet, disinfecting for 6 hours, taking out the raw materials, ventilating and resolving for 14 days, and finishing the whole disinfection.
And (3) detection:
the standard according to GB15979-2002 hygienic Standard for Disposable sanitary articles is shown in Table 1 below:
table 1: sanitary index for women's menstrual period sanitary article
1. The sanitary pads sterilized in the above example 2 and comparative example 1 were tested for microorganisms and the ethylene oxide residue was measured, and the results are shown in the following table 2:
TABLE 2
2. The antibacterial effect of the sanitary pad sterilized in the above example 2 is detected according to the appendix C5 of GB15979-2002 (taking staphylococcus aureus as an example), and the specific detection effect is shown in the following table 3:
TABLE 3
3. The disinfectant prepared in example 1 was tested for its effect of killing poliovirus:
measuring the amount of poliomyelitis in the sample before and after the action of the disinfectant by using a cell infection method; determining the infection titer of each group of viruses by taking cytopathic effect as a judgment index, and calculating the inactivation rate of the disinfectant on poliomyelitis, wherein the specific operation mode is as follows:
s1, taking out cryopreserved test host cells from liquid nitrogen, quickly melting the cells in warm water at 37 ℃, washing the cells twice by using a cell maintenance solution, transferring the cells into a culture bottle with 10ml of complete culture medium, observing the growth condition of the cells day by day, and when the cells grow to be full of a monolayer, using the cells for a disinfection test;
s2, taking out the poliomyelitis-1 strain frozen at low temperature, thawing in water bath at 37 ℃, diluting by 10 times with a cell maintenance solution, then inoculating into a cell bottle containing a full monolayer of cells, placing in a 37 ℃ incubator, adsorbing and growing with the cells, observing lesions day by day, harvesting the viruses when 3/4 of the cells are diseased, taking out the culture solution during harvesting, crushing the host cells by using ultrasonic waves or repeated freezing and thawing, centrifuging as soon as possible, subpackaging the supernatant containing the viruses into sterile centrifuge tubes (1.5 ml) according to 1.0ml per tube, and freezing and preserving at-80 ℃ for later use;
s3, taking the disinfectant prepared in the embodiment 1, adding 2 times of volume of purified water for dilution, and then carrying out water bath at 20 +/-1 ℃ for later use;
s4, mixing 100 mu l of organic interfering substance with 100 mu l of virus stock solution, acting in a water bath at 20 +/-1 ℃ for 5min, adding 0.8ml of disinfectant, immediately mixing uniformly, timing, acting for a specified time, immediately taking out 0.1ml, adding a neutralizer and uniformly mixing;
s5, replacing a disinfectant with deionized water to set a positive control group, adding a poliomyelitis suspension for experiment and culture in the same step as the experiment group, and observing the growth condition of poliomyelitis;
s6, using a complete culture medium without poliomyelitis as a negative control, and observing whether the used culture medium is polluted or not and whether cells grow well or not;
s7, respectively adopting an end point dilution method to carry out virus titer determination on each group;
specifically, the operation steps of the end point dilution method are as follows: firstly, carrying out 10-fold serial dilution on a sample to be titrated by using a cell maintenance culture solution, then titrating the amount of viruses remained in each dilution sample on a 96-well culture plate, wherein each dilution is 4 wells (each well should be full of a monolayer of host cells), placing the culture plate at 37 ℃ for 1-2 h to ensure that all the residual viruses are adsorbed on the cells, taking out the culture plate, replacing the cell maintenance culture solution, continuously placing the culture plate in a carbon dioxide incubator (37 ℃, 5-inch CO2) for culture, observing cytopathic effect under a microscope day by day, continuously observing for 3d, observing and recording the cytopathic effect hole by hole, wherein the virus infection titer is calculated by an end point dilution method: expressed as half cell infectious dose (TCID 50);
the detection shows that the killing rate of the disinfectant on viruses after 1min of contact is more than or equal to 99.9 percent.
It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.