CN115669656A - Application of ocimene in preparation of honeycomb beetle attractant - Google Patents

Application of ocimene in preparation of honeycomb beetle attractant Download PDF

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Publication number
CN115669656A
CN115669656A CN202211320977.2A CN202211320977A CN115669656A CN 115669656 A CN115669656 A CN 115669656A CN 202211320977 A CN202211320977 A CN 202211320977A CN 115669656 A CN115669656 A CN 115669656A
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China
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ocimene
bee
attractant
beetle
beetles
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CN202211320977.2A
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CN115669656B (en
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赵红霞
李良斌
武丽仙
许雅静
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Institute of Zoology of Guangdong Academy of Sciences
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Institute of Zoology of Guangdong Academy of Sciences
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Abstract

The invention discloses an application of ocimene in preparing a honeycomb beetle attractant. The ocimene provided by the invention is used as a component of the honeycomb beetle attractant, has the characteristics of simplicity and convenience in operation, wide effective concentration range, high fault tolerance rate, high attraction efficiency and the like, and can obviously reduce the use of chemical pesticides in bee pest control and solve the problem of pesticide residue of bee products by popularization and application. The invention has important value in biological control of the Beatles nidae and has wide economic, social and ecological values.

Description

Application of ocimene in preparation of honeycomb beetle attractant
Technical Field
The invention belongs to the field of pest control, and particularly relates to an application of ocimene in preparation of a beetle nidus attractant.
Background
The bees are one of important beneficial insects in the world, and have important agricultural value, ecological value and economic value. In modern agriculture, the bee industry is praised as the wing of agriculture, and can provide a series of green and healthy foods such as honey, royal jelly, bee pollen, propolis and the like for human beings, thereby providing considerable economic benefit for beekeepers. Meanwhile, bee pollination plays an important role in plant flowering and fruit bearing, and evidence indicates that two thirds of human food is related to bee pollination. The honeybee industry is affected by factors including habitat reduction, pest and disease damage, and pesticide action. The pest and disease damage is an important factor for reducing the number of bees, so that the pest and disease damage prevention and control of the bees are an important prerequisite for the healthy development of the bee industry.
Beetles are bee hives parasites native to the southern sahara region of africa and have become an important problem in the apiculture industry and in wild bee colonies through the expansion of the bee product trade. The honeycomb beetles feed on honey, pollen and bee larvae, and even can be induced to lure worker bees of the bees to feed the bees; it can penetrate honeycomb to destroy wax comb, resulting in fermentation of pollen and honey, and further result in honeycomb structure destruction, which can cause collapse of bee colony and bee escape. In 2017, the honeycomb beetles are found for the first time in the Shanshen City of Guangdong province in China, and have invaded the four provinces of Guangdong, guangxi, yunnan and Hainan province in China to date. Causing serious losses to the local bee-keeping industry and agriculture of four provinces. At present, china does not form a good control strategy for the small honeycomb beetles. The control is mainly carried out by using pesticides, but the use of pesticides easily has negative effects on bees and bee products.
Therefore, the development of the attractant which has an attraction effect on the pests is a prevention and control means for effectively controlling the pests and reducing the use of pesticides. Has important reference significance for enriching the control technology of the honeycomb beetles in China. Plays a key role in green production of bee products and realization of rich economic value and ecological value of bees.
Disclosure of Invention
The invention aims to provide application of ocimene in preparation of a honeycomb beetle attractant, wherein the ocimene can effectively attract adult honeycomb beetles, control the quantity of pests, reduce the use of chemical pesticides and ensure the health of bees and the safety of bee products.
In order to achieve the purpose, the invention provides application of ocimene in preparing a honeycomb beetle attractant.
Preferably, the efficacy component of the bee nest beetle attractant is a ocimene single component.
Preferably, the bee nest beetle attractant is a solution containing the efficacy component, the solvent of the solution being a volatile solvent.
Preferably, the concentration of ocimene in the solution is 10-100 mg/mL.
More preferably, the concentration of ocimene in the solution is 50 to 100mg/mL.
Preferably, the efficacy component of the beetle bee hive attractant consists of ocimene and methyl linoleate.
More preferably, the honeycomb beetle attractant contains 50-100 mg/mL of ocimene and 100mg/mL of methyl linoleate.
The invention also provides a method for attracting the beetles in the honeycomb, which is to load the ocimene or the small beetle attractant containing the ocimene on a carrier and attract the small beetles in the honeycomb by using the ocimene.
Preferably, the carrier is a container or an adsorbate.
Preferably, the carrier loaded with the ocimene or the ocimene-containing bee hive beetle attractant is placed at the entrance of the beehive or in the beehive, and traps are arranged to trap or kill the bee hive beetles.
The ocimene provided by the invention is used as a component of the honeycomb beetle attractant, has the characteristics of simplicity and convenience in operation, wide effective concentration range, high fault tolerance rate, high attracting efficiency and the like, and can be popularized and applied to obviously reduce the use of chemical pesticides in bee pest control and solve the problem of pesticide residue of bee products. The invention has important value in biological control of the Beehmeria xylostella and has wide economic, social and ecological values.
Drawings
FIG. 1 is a schematic view of a Y-type olfactometer for the behavioral determination of Beatles nidae.
FIG. 2 shows the EAG measurement of bee larva pheromone (methyl palmitate) by Bethania nidulans, S in the abscissa represents n-hexane control, and the number represents the concentration (mg/mL) of the compound to be measured.
FIG. 3 shows the EAG measurement of Beatles nidulans on bee larva pheromone (ethyl oleate), S in the abscissa represents n-hexane control, and the number represents the concentration (mg/mL) of the compound to be measured.
FIG. 4 shows the EAG measurement of Beatles nidulans on bee larva pheromone (ocimene), S in the abscissa represents n-hexane control, and the number represents the concentration (mg/mL) of the compound to be measured.
FIG. 5 shows the EAG measurement of bee larva pheromone (methyl linoleate) by Beatles honeycomb, S in the abscissa represents n-hexane control, and the number represents the concentration (mg/mL) of the compound to be measured.
FIG. 6 is a statistical graph of the behavioral response of beetles bee hives to bee larva pheromone (ocimene 100 mg/mL).
FIG. 7 is a statistical graph showing the behavioral response of Beatles nidae to bee larva pheromone (methyl linoleate 100 mg/mL).
Detailed Description
The following examples are intended to be further limiting and are not intended to be limiting.
The information on the semiochemicals used in the following examples is shown in table 1:
TABLE 1 test information Compound information
Numbering Name of Chinese English name Manufacturer(s) CAS
1 Palmitic acid methyl ester Methyl palmitate Aladdin 112-39-0
2 Oleic acid ethyl ester Ethyl oleate Aladdin 111-62-6
3 Ocimum basilicum Oocimene Aladdin 13877-91-3
4 Linoleic acid methyl ester Methyl linoleate Aladdin 112-63-0
Example 1: antennal potential (EAG) response to bee larva pheromone
(1) Female and male tentacles of 5-7 days old honeycomb beetles are cut down along the base part by using a scalpel, the tip part is cut off by 0.2mm, the tentacles are connected with two silver electrodes of the EAG by using conductive adhesive, and then the electrodes are inserted into an EAG probe.
(2) The tip of the Pasteur tube was inserted into a small hole (diameter: 2.5mm, distance: 11cm from the outlet of the gas stream of the steel tube) on a steel tube (diameter: 8mm, length: 14 cm) through which a continuous gas stream was passed.
(3) The continuous gas flow rate in the steel tube was 4mL/s, the wide end of the stimulation tube was connected to the hose, and the gas flow rate in the hose was also 4mL/s at the separation. The stimulation time was 500ms and the stimulation interval was 30s.
(4) And after the EAG signal is stable, recording the response value of the antenna potential. All the information compounds to be tested use n-hexane as a solvent, a filter paper strip dropwise added with 10 mu L of n-hexane is used as a contrast, each antenna is firstly contacted with the contrast, then the test is sequentially carried out according to the concentration of the information compounds to be tested from low to high, and finally the contrast is tested once again. The stimulation time is 0.5s, the interval time between two times of stimulation is more than or equal to 30s, and the number of the female beetle adults and the male beetle adults of the honeycomb are respectively more than or equal to 15.
(5) Relative antennal potential value = (reaction value of sample to be tested)/(absolute value of difference of two controls/2) × 100%
(6) And (3) measuring the antennal potential of the bee larva pheromone.
Statistical analysis As shown in FIGS. 2, 3, 4 and 5, the female and male hive beetles have stronger electrophysiological reactivity to ocimene and methyl linoleate, which are higher than the reactivity of methyl palmitate and ethyl oleate.
Example 2: the attraction of a specific concentration of a one-component semiochemical solution to beetles was determined.
The experimental method comprises the following steps: dissolving certain information compound in n-hexane to prepare single-component information compound solution with specific concentration, and using Y-type olfactometer as measuring device. As shown in FIG. 1, the Y-type tube has a diameter of 5mm, the main arm and the branch arm are both 5cm long, and both sides are connected with a Pasteur tube by rubber hoses respectively, and the Pasteur tube is connected with a flow meter, a distilled water humidifying bottle, an activated carbon air purifying bottle and an air pump in sequence by the rubber hoses. At 1cm 2 Qualitative filter paper is used as a reagent carrier, and 10 mu L of to-be-detected information compound solution is dripped; using n-hexane as solvent as control, filtering with n-hexane filter paper and the information compoundThe filter papers were placed in two pasteur tubes respectively, and the air flow rate was adjusted to 0.8L/min. The beetles were activated by a halogen lamp placed 30cm above the Y-tube. In the experiment, the honeycomb beetles are introduced from the pipe orifice of the main arm of the Y-shaped pipe, timing is started, selection is made at the bifurcation part of the Y-shaped pipe, the insects go to different branch arms, each insect records for 2min, climbs to 2.5cm of a certain branch arm and stays for more than 30s, the selection is recorded as the selection, and otherwise, the selection is regarded as no selection. And (3) replacing a new Y-shaped pipe for every 5 adult insects tested and changing the position of the Y-shaped pipe to eliminate the possible influence of the position of a pipe arm and residual smell on behaviors, and cleaning and airing the replaced Y-shaped pipe by using methanol for later use. Each group tested 20 adults, which were repeated 3 times.
Preparing tested information compounds (ocimene and methyl linoleate) into single-component compound solutions with the concentrations of 100mg of compound/mL of n-hexane, 50mg of compound/mL of n-hexane and 10mg of compound/mL of n-hexane, and then respectively operating according to the methods to determine the behavior reaction of the beetles on the tested information compounds.
Statistical analysis results are shown in FIGS. 6 and 7, where the right side is the n-hexane control, the left side is the test semiochemical, and the abscissa indicates the number of beetle heads selecting the odor.
The test results show that: octrelene at a concentration of 100mg/mL had a very significant attraction to both female and male beetles nidus vespae compared to the control (n-hexane). Methyl linoleate at a concentration of 100mg/mL had only a very significant attracting effect on male beetles nidus vespae. Octrelene with the concentration of 100mg/mL has extremely remarkable attraction effect on both male and female honeycomb beetles. Octrelene at a concentration of 50mg/mL had a significant attractive effect on Beatles nidulans. The ethyl linoleate at a concentration of 50mg/mL had no attraction effect on both males and females.

Claims (10)

1. Application of ocimene in preparing bee nest beetle attractant is provided.
2. The use as claimed in claim 1 wherein the efficacy component of the beetle attractant is a sole component of ocimene.
3. The use as claimed in claim 1 or claim 2 wherein the beetle bee attractant is a solution containing ocimene, the solvent of the solution being a volatile solvent.
4. The use according to claim 3, wherein the concentration of ocimene in the solution is 10-100 mg/mL.
5. The use according to claim 4, wherein the concentration of ocimene in the solution is 50-100 mg/mL.
6. The use as claimed in claim 1 wherein the efficacy component of the beetle bee attractant consists of ocimene and methyl linoleate.
7. The use as claimed in claim 6, wherein the beetle bee hive attractant comprises 50-100 mg/mL ocimene and 100mg/mL methyl linoleate.
8. A method for attracting small beetles in nidus Vespae is characterized in that ocimene or an attractant containing ocimene for small beetles in nidus Vespae is loaded on a carrier, and the ocimene is used for attracting the small beetles in nidus Vespae.
9. The method of claim 8, wherein the support is a container or an adsorbate.
10. The method as claimed in claim 8 or 9, wherein the carrier loaded with ocimene or ocimene-containing beetle attractant is placed at the entrance of the beehive or inside the beehive, and a trap is provided to trap or kill the beetles.
CN202211320977.2A 2022-10-26 2022-10-26 Application of ocimene in preparation of small honeycomb beetles attractant Active CN115669656B (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116473057A (en) * 2023-06-15 2023-07-25 广东省林业科学研究院 Application of ethyl oleate in attracting solenopsis invicta

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109907075A (en) * 2019-03-12 2019-06-21 广东省生物资源应用研究所 A kind of trapping composition and application method for preventing and treating the small beetle of beehive

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109907075A (en) * 2019-03-12 2019-06-21 广东省生物资源应用研究所 A kind of trapping composition and application method for preventing and treating the small beetle of beehive

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
金涛;钟义海;林玉英;彭正强;韩文素;高景林;: "防治新入侵害虫蜂巢小甲虫杀虫剂的室内筛选", 环境昆虫学报, no. 03 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116473057A (en) * 2023-06-15 2023-07-25 广东省林业科学研究院 Application of ethyl oleate in attracting solenopsis invicta
CN116473057B (en) * 2023-06-15 2023-09-08 广东省林业科学研究院 Application of ethyl oleate in attracting solenopsis invicta

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