CN115667519A - 含i型聚酮合酶基因的质粒的制备方法 - Google Patents

含i型聚酮合酶基因的质粒的制备方法 Download PDF

Info

Publication number
CN115667519A
CN115667519A CN202180037678.5A CN202180037678A CN115667519A CN 115667519 A CN115667519 A CN 115667519A CN 202180037678 A CN202180037678 A CN 202180037678A CN 115667519 A CN115667519 A CN 115667519A
Authority
CN
China
Prior art keywords
dna
pks
plasmid
encoding
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180037678.5A
Other languages
English (en)
Chinese (zh)
Inventor
大卫·利普斯
海斯·韦尔卡雷吉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Spiber Inc
Original Assignee
Spiber Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spiber Inc filed Critical Spiber Inc
Publication of CN115667519A publication Critical patent/CN115667519A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20Bacteria; Culture media therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/11DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52Genes encoding for enzymes or proenzymes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1025Acyltransferases (2.3)
    • C12N9/1029Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y203/00Acyltransferases (2.3)
    • C12Y203/01Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C12Y203/010946-Deoxyerythronolide-B synthase (2.3.1.94)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/04Actinomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07Bacillus
    • C12R2001/125Bacillus subtilis ; Hay bacillus; Grass bacillus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/185Escherichia
    • C12R2001/19Escherichia coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12RINDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00Microorganisms ; Processes using microorganisms
    • C12R2001/01Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465Streptomyces

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202180037678.5A 2020-05-26 2021-05-25 含i型聚酮合酶基因的质粒的制备方法 Pending CN115667519A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2020-091798 2020-05-26
JP2020091798 2020-05-26
PCT/JP2021/019819 WO2021241584A1 (ja) 2020-05-26 2021-05-25 I型ポリケチドシンターゼ遺伝子を含むプラスミドの調製方法

Publications (1)

Publication Number Publication Date
CN115667519A true CN115667519A (zh) 2023-01-31

Family

ID=78744095

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180037678.5A Pending CN115667519A (zh) 2020-05-26 2021-05-25 含i型聚酮合酶基因的质粒的制备方法

Country Status (5)

Country Link
US (1) US20230174997A1 (https=)
EP (1) EP4159861A4 (https=)
JP (1) JPWO2021241584A1 (https=)
CN (1) CN115667519A (https=)
WO (1) WO2021241584A1 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115698297A (zh) * 2020-05-26 2023-02-03 丝芭博株式会社 多模块生物合成酶基因组合文库的制备方法

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117280036A (zh) * 2021-05-25 2023-12-22 丝芭博株式会社 质粒的制备方法及质粒

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1292031A (zh) * 1998-02-26 2001-04-18 诺沃诺尔迪斯克生物技术有限公司 在芽孢杆菌属细胞中生产多肽的方法
CN106170547A (zh) * 2014-01-21 2016-11-30 高效基因设计技术研究协会 单元dna组合物的制备方法及dna连结体的制作方法
CN115698297A (zh) * 2020-05-26 2023-02-03 丝芭博株式会社 多模块生物合成酶基因组合文库的制备方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2618699C (en) 2005-08-11 2012-10-02 J. Craig Venter Institute, Inc. In vitro recombination method
US20100291633A1 (en) 2007-09-03 2010-11-18 Thorsten Selmer Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases
AU2009214435C1 (en) 2008-02-15 2014-07-17 Synthetic Genomics, Inc. Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
CN103060362A (zh) * 2012-12-03 2013-04-24 复旦大学 一种基于位点特异性重组的抗生素生物合成基因簇的克隆方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1292031A (zh) * 1998-02-26 2001-04-18 诺沃诺尔迪斯克生物技术有限公司 在芽孢杆菌属细胞中生产多肽的方法
CN106170547A (zh) * 2014-01-21 2016-11-30 高效基因设计技术研究协会 单元dna组合物的制备方法及dna连结体的制作方法
CN115698297A (zh) * 2020-05-26 2023-02-03 丝芭博株式会社 多模块生物合成酶基因组合文库的制备方法

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JANA KUMPFMÜLLER等: "Production of the polyketide 6-deoxyerythronolide B in the heterologous host Bacillus subtilis", APPL MICROBIOL BIOTECHNOL, vol. 100, 2 October 2015 (2015-10-02), pages 1214 *
柘植谦尔等: "枯草菌による遺伝子集積法のOGAB法による長鎖DNA合成", 生物工学, vol. 93, 25 September 2015 (2015-09-25), pages 528 - 529 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115698297A (zh) * 2020-05-26 2023-02-03 丝芭博株式会社 多模块生物合成酶基因组合文库的制备方法

Also Published As

Publication number Publication date
WO2021241584A1 (ja) 2021-12-02
US20230174997A1 (en) 2023-06-08
EP4159861A4 (en) 2024-06-05
EP4159861A1 (en) 2023-04-05
JPWO2021241584A1 (https=) 2021-12-02

Similar Documents

Publication Publication Date Title
Rodriguez et al. Rapid engineering of polyketide overproduction by gene transfer to industrially optimized strains
EP4159862A1 (en) Method for preparing combinatorial library of multi-modular biosynthetic enzyme gene
JPH09224687A (ja) ポリケチドシンターゼ遺伝子
JPH09224686A (ja) プラテノリドシンターゼ遺伝子
US20030087405A1 (en) Products encoded by a methymycin/pikromycin biosynthetic gene cluster
CN112080454B (zh) 一株产柔红霉素的工程链霉菌及其构建方法
CN109486848A (zh) 一种含有多杀菌素多操纵子人工基因簇质粒的构建方法与应用
Wu et al. Toward improvement of erythromycin A production in an industrial Saccharopolyspora erythraea strain via facilitation of genetic manipulation with an artificial attB site for specific recombination
Gao et al. Challenges of functional expression of complex polyketide biosynthetic gene clusters
CN115667519A (zh) 含i型聚酮合酶基因的质粒的制备方法
Li et al. Genome-based rational engineering of Actinoplanes deccanensis for improving fidaxomicin production and genetic stability
HUT54416A (en) Process for utilizing placespecific integrating function of fag fi-c-31
CN103183729B (zh) 谷氏菌素生物合成相关蛋白系统及其编码基因簇与应用
WO2024062992A1 (ja) Dna配列のスクリーニング方法
Wilkinson et al. Biocatalysis in pharmaceutical preparation and alteration
CN101892186B (zh) 产表柔红霉素的天蓝淡红链霉菌的基因工程菌及其制备方法
CN101892185B (zh) 产表柔红霉素的天蓝淡红链霉菌基因工程菌及其制备方法
JP7370121B2 (ja) プラスミドの製造方法及びプラスミド
CN116426454A (zh) 一种提高阿维菌素B1a组分效价和比例的基因工程方法
He et al. dTSR enables efficient improvement of heterologous production of spinosad in Saccharopolyspora erythraea
WO2004015088A2 (en) Transposon-based transformation system
KR100636653B1 (ko) 신규한 올리보실 피크로마이신 유도체 및 그 제조방법
KR100649394B1 (ko) 신규한 올리보실 메티마이신 유도체 및 그 제조방법
CN113462704B (zh) 一种植物细胞分裂素狭霉素的生物合成基因簇及其生物材料以及在合成狭霉素中的应用
JP4633519B2 (ja) ミデカマイシン高生産菌

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination