CN115667519A - 含i型聚酮合酶基因的质粒的制备方法 - Google Patents
含i型聚酮合酶基因的质粒的制备方法 Download PDFInfo
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- CN115667519A CN115667519A CN202180037678.5A CN202180037678A CN115667519A CN 115667519 A CN115667519 A CN 115667519A CN 202180037678 A CN202180037678 A CN 202180037678A CN 115667519 A CN115667519 A CN 115667519A
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- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N9/10—Transferases (2.)
- C12N9/1025—Acyltransferases (2.3)
- C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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- C12Y203/00—Acyltransferases (2.3)
- C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
- C12Y203/01094—6-Deoxyerythronolide-B synthase (2.3.1.94)
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
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Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2020-091798 | 2020-05-26 | ||
| JP2020091798 | 2020-05-26 | ||
| PCT/JP2021/019819 WO2021241584A1 (ja) | 2020-05-26 | 2021-05-25 | I型ポリケチドシンターゼ遺伝子を含むプラスミドの調製方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115667519A true CN115667519A (zh) | 2023-01-31 |
Family
ID=78744095
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202180037678.5A Pending CN115667519A (zh) | 2020-05-26 | 2021-05-25 | 含i型聚酮合酶基因的质粒的制备方法 |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20230174997A1 (https=) |
| EP (1) | EP4159861A4 (https=) |
| JP (1) | JPWO2021241584A1 (https=) |
| CN (1) | CN115667519A (https=) |
| WO (1) | WO2021241584A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115698297A (zh) * | 2020-05-26 | 2023-02-03 | 丝芭博株式会社 | 多模块生物合成酶基因组合文库的制备方法 |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN117280036A (zh) * | 2021-05-25 | 2023-12-22 | 丝芭博株式会社 | 质粒的制备方法及质粒 |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1292031A (zh) * | 1998-02-26 | 2001-04-18 | 诺沃诺尔迪斯克生物技术有限公司 | 在芽孢杆菌属细胞中生产多肽的方法 |
| CN106170547A (zh) * | 2014-01-21 | 2016-11-30 | 高效基因设计技术研究协会 | 单元dna组合物的制备方法及dna连结体的制作方法 |
| CN115698297A (zh) * | 2020-05-26 | 2023-02-03 | 丝芭博株式会社 | 多模块生物合成酶基因组合文库的制备方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2618699C (en) | 2005-08-11 | 2012-10-02 | J. Craig Venter Institute, Inc. | In vitro recombination method |
| US20100291633A1 (en) | 2007-09-03 | 2010-11-18 | Thorsten Selmer | Method of cloning at least one nucleic acid molecule of interest using type iis restriction endonucleases, and corresponding cloning vectors, kits and system using type iis restriction endonucleases |
| AU2009214435C1 (en) | 2008-02-15 | 2014-07-17 | Synthetic Genomics, Inc. | Methods for in vitro joining and combinatorial assembly of nucleic acid molecules |
| CN103060362A (zh) * | 2012-12-03 | 2013-04-24 | 复旦大学 | 一种基于位点特异性重组的抗生素生物合成基因簇的克隆方法 |
-
2021
- 2021-05-25 US US17/926,204 patent/US20230174997A1/en active Pending
- 2021-05-25 EP EP21814439.2A patent/EP4159861A4/en active Pending
- 2021-05-25 CN CN202180037678.5A patent/CN115667519A/zh active Pending
- 2021-05-25 WO PCT/JP2021/019819 patent/WO2021241584A1/ja not_active Ceased
- 2021-05-25 JP JP2022526587A patent/JPWO2021241584A1/ja active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1292031A (zh) * | 1998-02-26 | 2001-04-18 | 诺沃诺尔迪斯克生物技术有限公司 | 在芽孢杆菌属细胞中生产多肽的方法 |
| CN106170547A (zh) * | 2014-01-21 | 2016-11-30 | 高效基因设计技术研究协会 | 单元dna组合物的制备方法及dna连结体的制作方法 |
| CN115698297A (zh) * | 2020-05-26 | 2023-02-03 | 丝芭博株式会社 | 多模块生物合成酶基因组合文库的制备方法 |
Non-Patent Citations (2)
| Title |
|---|
| JANA KUMPFMÜLLER等: "Production of the polyketide 6-deoxyerythronolide B in the heterologous host Bacillus subtilis", APPL MICROBIOL BIOTECHNOL, vol. 100, 2 October 2015 (2015-10-02), pages 1214 * |
| 柘植谦尔等: "枯草菌による遺伝子集積法のOGAB法による長鎖DNA合成", 生物工学, vol. 93, 25 September 2015 (2015-09-25), pages 528 - 529 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115698297A (zh) * | 2020-05-26 | 2023-02-03 | 丝芭博株式会社 | 多模块生物合成酶基因组合文库的制备方法 |
Also Published As
| Publication number | Publication date |
|---|---|
| WO2021241584A1 (ja) | 2021-12-02 |
| US20230174997A1 (en) | 2023-06-08 |
| EP4159861A4 (en) | 2024-06-05 |
| EP4159861A1 (en) | 2023-04-05 |
| JPWO2021241584A1 (https=) | 2021-12-02 |
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