CN115639163A - Hemoglobin rapid detection device based on intelligent mobile terminal and application thereof - Google Patents
Hemoglobin rapid detection device based on intelligent mobile terminal and application thereof Download PDFInfo
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- CN115639163A CN115639163A CN202211107876.7A CN202211107876A CN115639163A CN 115639163 A CN115639163 A CN 115639163A CN 202211107876 A CN202211107876 A CN 202211107876A CN 115639163 A CN115639163 A CN 115639163A
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Abstract
The invention discloses a hemoglobin rapid detection device based on an intelligent mobile terminal and application thereof, wherein the hemoglobin rapid detection device comprises a shell, a hemoglobin detection chip and the intelligent mobile terminal, wherein a light source and a battery are arranged in the shell; a reading area made of transparent materials is arranged on the hemoglobin detection chip, a micro-groove for storing a hemoglobin detection reagent is arranged in the reading area, and a blood sample can enter the micro-groove through the siphon action at the opening of the micro-groove and react with the hemoglobin detection reagent; be equipped with chip inserted hole and intelligent Mobile terminal standing groove on the shell, install the application that is used for reading and takes notes incident light and transmitted light intensity in the intelligent Mobile terminal, the application can calculate hemoglobin concentration according to the standard curve that predetermines to show on the intelligent Mobile terminal screen. The detection device is portable, simple and quick to operate, high in accuracy and capable of being applied to anemia diagnosis and treatment effect evaluation in primary medical institutions or in households.
Description
Technical Field
The invention relates to the technical field of biochemical detection equipment, in particular to a hemoglobin rapid detection device based on an intelligent mobile terminal and application thereof.
Background
Anemia, defined as a decrease in circulating red blood cell or hemoglobin (Hgb) concentration, is one of the most common diseases. Hemoglobin is a main index for diagnosing anemia, and for people with high anemia, regular detection of hemoglobin has important significance for anemia screening and treatment effect evaluation. At present, the clinical hemoglobin detection needs professional large-scale equipment and operators, and cannot be used in primary medical institutions and households. Therefore, there is a need to develop a new detection device which is simple and fast to operate, has high accuracy, and can be used for diagnosing anemia and evaluating the treatment effect in primary medical institutions or at home.
Disclosure of Invention
In view of the above disadvantages of the prior art, the present invention is directed to a hemoglobin rapid detection device based on an intelligent mobile terminal and an application thereof.
In order to achieve the above and other related objects, the present invention provides a hemoglobin rapid detection apparatus based on an intelligent mobile terminal of an intelligent mobile terminal, which has the following basic scheme:
the detection device comprises a shell, a hemoglobin detection chip and an intelligent mobile terminal, wherein a light source and a battery are installed in the shell, and the battery is used for supplying power to the light source;
the hemoglobin detection chip is provided with a reading area, the reading area is made of transparent materials, the reading area is provided with a microgroove, a hemoglobin detection reagent is stored in the microgroove, and a blood sample can enter the microgroove through the siphoning action at the notch of the microgroove and react with the hemoglobin detection reagent;
the shell is provided with a chip insertion hole and an intelligent mobile terminal placing groove, and the chip insertion hole is positioned above the intelligent mobile terminal placing groove; the intelligent mobile terminal is internally provided with an ambient light sensor, the light source is positioned above the chip insertion hole and used for emitting light to the reading area of the hemoglobin detection chip, and when the intelligent mobile terminal is placed in the placing groove, the light emitted by the light source is aligned with the ambient light sensor internally arranged in the intelligent mobile terminal;
the intelligent mobile terminal is internally provided with an application program for reading and recording incident light and transmitted light intensity, and the application program can calculate the concentration of hemoglobin according to a preset standard curve and display the concentration on a screen of the intelligent mobile terminal.
The working principle of the basic scheme is as follows:
firstly, adding a blood sample into a microgroove of a reading area of a hemoglobin detection chip, wherein two specific modes are as follows: 1. contacting the notch of the micro-groove with fingertip blood, and dripping a blood sample to the notch of the micro-groove by using a pipettor; by adopting the two modes, after the blood sample is dripped into the opening of the micro-groove, the blood sample can be sucked into the micro-groove under the siphon action, the hemoglobin detection reagent stored in the micro-groove cracks blood cells, and a series of chemical reactions occur to enable hemoglobin to generate a specific absorption peak; placing the intelligent mobile terminal in the placing groove, aligning an ambient light sensor with the light source, turning on the light source, reading and recording the intensity of the excitation light; inserting the reading area of the hemoglobin detection chip into the chip insertion opening, wherein the reading area is made of transparent materials, so that light rays emitted by the light source can penetrate through the reading area, and the intelligent mobile terminal can read and record the transmitted light intensity; and finally, calculating the concentration of the hemoglobin by an application program installed in the intelligent mobile terminal according to a preset standard curve, and displaying the concentration of the hemoglobin on a screen of the intelligent mobile terminal, so that the detection result of the hemoglobin is simply and quickly obtained.
The method for making the standard curve is well known to those skilled in the art, and specifically comprises the following steps: according to incident light I 0 And transmitting light I, calculating absorbance through the Lambert-beer law, and fitting a linear regression equation of the concentration of the hemoglobin and the absorbance to obtain a standard curve.
The application program mentioned in the invention can be written by itself, and can download the existing application program, such as phyhox.
Optionally, the preparation method of the hemoglobin detection reagent in the micro-chamber comprises: mixing a red blood cell lysate and a hemoglobin biochemical analysis reagent to prepare a hemoglobin detection solution, injecting the hemoglobin detection solution into a micro-groove through the siphoning action of the micro-groove, drying the solution by a vacuum freeze drying technology, and storing the dried solution in the micro-groove, wherein the red blood cell lysate is selected from any one of SDS, NP-40, tritonX-100 and sodium deoxycholate, and the hemoglobin biochemical analysis reagent is a detection reagent used in any one of an azide methemoglobin (HiN 3) determination method, a bromohexadecyltrimethylamine (CTAB) hemoglobin determination method and an alkali-hydroxyhemoglobin (AHD 575) determination method; more preferably, the hemoglobin detection solution comprises: 500ng/mL of deoxybilirubin, 300ng/mL of sodium nitrite, 300ng/mL of sodium azide and 250ng/mL of Triton-X.
Optionally, the micro-groove can contain 5-50 microliter of liquid, and the depth of the micro-groove is 50-100 micrometers.
Optionally, the transparent material is selected from any one of polymethyl methacrylate, polystyrene and polycarbonate.
Optionally, the hemoglobin detecting chip is of a strip card type structure, one end of the chip is a reading area, and the other end of the chip is a holding area. The handheld area is arranged, so that the hemoglobin detection chip can be conveniently and manually inserted into the chip insertion opening.
Optionally, the light source is selected from any one of a laser, an LED lamp, and a white light source processed by a grating and a filter.
Optionally, the housing is made of a material that is opaque to light to avoid interference from ambient light.
Optionally, a switch is disposed on the housing, and the switch is used for controlling the light source to be turned on and off.
Optionally, the detection device further comprises a voltage regulator for regulating the voltage of the battery. Typically, the voltage is regulated to the rated voltage of the lithium battery, such as 3.7V.
Optionally, the battery is a disposable lithium battery or a rechargeable lithium battery.
Optionally, the detection apparatus further includes a charging module, the charging module is installed in the housing, and the charging module is connected to the rechargeable lithium battery so as to charge the rechargeable lithium battery.
Optionally, the smart mobile terminal includes, but is not limited to, mobile applications such as a smartphone, a tablet computer, and a notebook computer, which are commonly available in the market, and is equipped with an ambient light sensor, and the hemoglobin detection chip can be read by the ambient light sensor to detect absorbance.
The invention also provides a hemoglobin detection method, which adopts the device to detect the concentration of hemoglobin.
As mentioned above, the hemoglobin rapid detection device based on the intelligent mobile terminal and the application thereof have the following beneficial effects:
the hemoglobin detection device based on the intelligent mobile terminal has the advantages of portability, simplicity and rapidness in operation, convenience in use, high accuracy and the like, and can be applied to anemia diagnosis and treatment effect evaluation in basic medical institutions or in households.
Drawings
Fig. 1 is a schematic view of an overall structure of a hemoglobin rapid detection apparatus based on an intelligent mobile terminal according to an embodiment of the present invention.
Fig. 2 is a schematic diagram illustrating an internal structure of a housing of a hemoglobin rapid detection apparatus based on an intelligent mobile terminal according to an embodiment of the present invention.
Fig. 3 is a partial cross-sectional view of a hemoglobin rapid detection apparatus based on an intelligent mobile terminal according to an embodiment of the present invention.
Fig. 4 is a schematic perspective view illustrating a hemoglobin detecting chip of a hemoglobin rapid detection apparatus based on an intelligent mobile terminal according to an embodiment of the present invention.
Fig. 5 is a diagram illustrating a size structure of a hemoglobin detection chip of a hemoglobin rapid detection apparatus based on an intelligent mobile terminal according to an embodiment of the present invention.
FIG. 6 is a standard graph in example 1 of the present invention.
Description of reference numerals:
the intelligent mobile terminal comprises a shell 1, a chip insertion port 101, an intelligent mobile terminal placing groove 102, a hemoglobin detection chip 2, a reading area 201, a microgroove 2011, a handheld area 202, an intelligent mobile terminal 3, a light source 4, a battery 5, a switch 6, a voltage regulator 7 and a charging module 8.
Detailed Description
The following embodiments of the present invention are provided by way of specific examples, and other advantages and effects of the present invention will be readily apparent to those skilled in the art from the disclosure herein. The invention is capable of other and different embodiments and of being practiced or of being carried out in various ways, and its several details are capable of modification in various respects, all without departing from the spirit and scope of the present invention.
It should be noted that the drawings provided in the present embodiment are only for illustrating the basic idea of the present invention, and the components related to the present invention are only shown in the drawings rather than drawn according to the number, shape and size of the components in actual implementation, and the type, quantity and proportion of the components in actual implementation may be changed freely, and the layout of the components may be more complicated. The structures, the proportions, the sizes, and the like shown in the drawings attached to the present specification are only used for matching with the disclosure of the present specification, so as to be understood and read by those skilled in the art, and are not used for limiting the conditions under which the present invention can be implemented, so that the present invention has no technical essence, and any structural modification, changes of the proportion relation, or adjustment of the size, should fall within the scope of the technical disclosure of the present invention without affecting the efficacy and the achievable purpose of the present invention. In addition, the terms "upper", "lower", "left", "right", "middle" and "one" used in the present specification are used for clarity of description, and are not intended to limit the scope of the present invention, and the relative relationship between the terms and the terms may be changed or adjusted without substantial change in the technical content.
As shown in fig. 1, fig. 2 and fig. 3, an embodiment of the present invention provides an intelligent mobile terminal-based hemoglobin rapid detection apparatus, including a housing 1, a hemoglobin detection chip 2 and an intelligent mobile terminal 3, wherein a light source 4 and a battery 5 are installed in the housing 1, and the battery 5 is used for supplying power to the light source 4.
As shown in fig. 2, a reading area 201 is provided on the hemoglobin detection chip 2, the reading area 201 is made of a transparent material, the reading area 201 is provided with a microgroove 2011, a hemoglobin detection reagent is stored in the microgroove 2011, and a blood sample can enter the microgroove 2011 through siphon action at the notch of the microgroove 2011 to react with the hemoglobin detection reagent.
The shell 1 is provided with a chip insertion hole 101 and an intelligent mobile terminal placing groove 102, and the chip insertion hole 101 is positioned above the intelligent mobile terminal placing groove 102; the intelligent mobile terminal 3 is internally provided with an ambient light sensor, the light source 4 is positioned above the chip insertion hole 101 and used for emitting light to the reading area 201 of the hemoglobin detection chip 2, and when the intelligent mobile terminal 3 is placed in the placing groove, the light emitted by the light source 4 is aligned with the ambient light sensor internally arranged in the intelligent mobile terminal 3.
An application program for reading and recording incident light and transmitted light intensity is installed in the intelligent mobile terminal 3, and the application program can calculate hemoglobin concentration according to a preset standard curve and display the hemoglobin concentration on a screen of the intelligent mobile terminal 3.
The method for making the standard curve mentioned in the embodiment of the invention is well known by the technicians in the field, and specifically comprises the following steps: and calculating the absorbance through the Lambert-beer law according to the incident light I0 and the transmitted light I, and then fitting a linear regression equation of the hemoglobin concentration and the absorbance to obtain a standard curve.
The application program in the mentioned embodiment of the present invention can be written by itself, and can download the existing application program, such as phyhox.
Further, the preparation method of the hemoglobin detection reagent in the micro-chamber 2011 includes: mixing the erythrocyte lysate and the hemoglobin biochemical analysis reagent to prepare a hemoglobin detection solution, injecting the hemoglobin detection solution into the microgroove 2011 under the siphoning action of the microgroove 2011, drying the hemoglobin detection solution by a vacuum freeze drying technology, and storing the dried hemoglobin detection solution in the microgroove 2011; the prepared hemoglobin detection chip 2 can be put into a vessel with a drying bag for storage for later use. The erythrocyte lysate is selected from any one of SDS, NP-40, tritonX-100 and sodium deoxycholate, and the hemoglobin biochemical analysis reagent is a detection reagent used in any one of azide methemoglobin (HiN 3) determination method, bromohexadecyltrimethylamine (CTAB) hemoglobin determination method and alkali-hydroxyhemoglobin (AHD 575) determination method. For example, hemoglobin detection solutions include: 500ng/mL of deoxybilirubin, 300ng/mL of sodium nitrite, 300ng/mL of sodium azide and 250ng/mL of Triton-X. The erythrocyte lysate and the hemoglobin biochemical analysis reagent adopted by the embodiment of the invention belong to reagents used in a general erythrocyte lysis method and a hemoglobin determination method in the field, and can be adjusted at will by a person skilled in the art according to actual conditions, and are not limited to the formula of the hemoglobin detection solution.
Further, the hemoglobin detecting chip 2 is a strip card structure, one end of which is a reading area 201, and the other end of which is a holding area 202. The holding section 202 is provided to facilitate the holding of the hemoglobin detecting chip 2 into the chip insertion port 101. The micro grooves 2011 can hold 5-50 microliters of liquid, and the depth of the micro grooves 2011 is 50-100 micrometers. Specifically, the chip may be fabricated with reference to the dimensions shown in fig. 5, but is not limited thereto.
Further, the transparent material used for the reading area 201 of the hemoglobin detecting chip 2 can be selected from any one of polymethyl methacrylate, polystyrene and polycarbonate. The hemoglobin detection chip 2 can be manufactured by injection molding technology.
Further, the light source 4 should correspond to the maximum absorption peak of the hemoglobin detection reagent, and a laser, an LED lamp, or a white light source 4 processed by a grating and a filter may be selected.
Further, the housing 1 should be made of a light-tight material, such as a black material, or a light-tight plastic, to avoid interference from ambient light. The shell 1 model of the hemoglobin rapid detection device is designed according to the position of the intelligent mobile terminal 3 environment light sensor, the hemoglobin rapid detection device is manufactured by using a 3D printing technology, and then electronic elements such as a light source 4, a battery 5 and a switch 6 are sequentially installed to construct the complete hemoglobin rapid detection device.
Further, a switch 6 is disposed on the housing 1, and the light source 4, the battery 5, the switch 6, etc. form a series circuit, so as to control the turning on and off of the light source 4 through the switch 6, and the switch 6 may be a push switch 6.
Further, the detection device further comprises a voltage regulator 7, and the voltage regulator 7 is used for regulating the voltage of the battery 5. Typically, the voltage is regulated to the rated voltage of the lithium battery 5, such as 3.7V.
Further, the battery 5 is a disposable lithium battery 5 or a rechargeable lithium battery 5. If the rechargeable lithium battery 5 is adopted, the detection device further comprises a charging module 8, the charging module 8 is installed in the shell 1, and the charging module 8 is connected with the rechargeable lithium battery 5 so as to charge the rechargeable lithium battery 5.
Further, the smart mobile terminal 3 includes but is not limited to mobile applications such as smart phones, tablet computers, and notebooks that are commonly available in the market, and is installed with an ambient light sensor, and can read the absorbance of the hemoglobin detection chip 2 through the ambient light sensor.
The hemoglobin rapid detection device provided by the embodiment of the invention is suitable for detecting hemoglobin in venous blood and fingertip blood, the working principle is that an ambient light sensor of an intelligent mobile terminal 3 is utilized to read the absorbance of a hemoglobin detection chip 2, and the specific use method is as follows:
firstly, a blood sample is added into a microgroove 2011 of a reading area 201 of a hemoglobin detection chip 2, and two specific modes are provided: 1. contacting the notch of the microgroove 2011 with fingertip blood, and dripping a blood sample to the notch of the microgroove 2011 by using a pipette; by adopting the two modes, after the blood sample is dripped to the notch of the microgroove 2011, the blood sample can be sucked into the microgroove 2011 under the siphon action, the hemoglobin detection reagent stored in the microgroove 2011 enables blood cells to be cracked, and a series of chemical reactions occur to enable hemoglobin to generate a specific absorption peak; placing the intelligent mobile terminal 3 in a placing groove, aligning an ambient light sensor with the light source 4, turning on the light source 4, reading and recording the intensity of excitation light; then, the reading area 201 of the hemoglobin detection chip 2 is inserted into the chip insertion port 101, and since the reading area 201 is made of transparent material, the light emitted by the light source 4 can penetrate through the reading area 201, and the intelligent mobile terminal 3 can read the intensity of the transmitted light and record the intensity of the transmitted light; and finally, calculating the concentration of the hemoglobin by an application program installed in the intelligent mobile terminal 3 according to a preset standard curve, and displaying the concentration of the hemoglobin on a screen of the intelligent mobile terminal 3, so that the detection result of the hemoglobin is simply and quickly obtained.
Another embodiment of the present invention further provides a hemoglobin detection method, which uses the apparatus provided in the above embodiment to detect hemoglobin concentration, so as to diagnose whether anemia exists. The sensitivity of the method for diagnosing anemia provided by the embodiment of the invention is more than 95%, and the specificity is more than 96%.
The following lists embodiments of detecting the hemoglobin concentration of an actual blood sample by using the hemoglobin rapid detection device based on the smart mobile terminal in the above embodiments. It should be understood that the following examples are illustrative only and are not to be construed as limiting the scope of the invention, and that numerous insubstantial modifications and adaptations of the invention as described above will now occur to those skilled in the art. The specific process parameters and the like of the following examples are also only one example of suitable ranges, i.e., those skilled in the art can select the appropriate ranges through the description herein, and are not limited to the specific values exemplified below.
Example 1
Detecting hemoglobin concentration in venous blood
(1) Manufacturing a hemoglobin detection chip of polystyrene by an injection molding technology, wherein the thickness of a microgroove in a reading area of the chip is 100 micrometers;
(2) A hemoglobin detection solution was prepared, wherein 1mL of the solution contained 500ng of deoxycholic acid, 300ng of sodium nitrite, 300ng of sodium azide, and 250ng of Triton-X100.
(3) Immersing the hemoglobin detection chip into a hemoglobin detection solution, wherein about 10 microliters of the hemoglobin detection solution enters a microgroove of the hemoglobin detection chip under the siphon action, and then taking the hemoglobin detection chip out of the solution and placing the chip into a vacuum freeze dryer for processing. And finally, placing the hemoglobin detection chip in a vessel with a drying bag for storage for later use.
(4) A model of hemoglobin detection equipment is designed according to the position of an ambient light sensor of the smart phone, the model is manufactured by using a 3D printing technology, and then electronic elements such as LED lamp beads, a battery, a switch and a voltage regulator are sequentially installed to construct a complete hemoglobin rapid detection device.
(5) When hemoglobin is detected, firstly, a switch of the detection device is turned on, then, a mobile phone application program is operated, and at the moment, the application program can read exciting light I 0 。
(6) A venous blood sample stored in an EDTA anticoagulant tube is taken, after being uniformly mixed, 10 microliter of blood sample is added into a notch of a chip micro-groove and then enters the micro-groove under the siphon action, a hemoglobin detection reagent stored in the micro-groove can crack blood cells, and a series of chemical reactions occur to enable hemoglobin to generate a specific absorption peak.
(7) For 1min, insert the chip into the chip insert port on the detection device, at this time, the application program will record the transmitted light intensity I, and finally follow the built-in standard curve (as shown in fig. 6, y =0.00748x +0.106, r +) 2 = 0.099), and displayed on the screen of the mobile phone.
The foregoing embodiments are merely illustrative of the principles and utilities of the present invention and are not intended to limit the invention. Any person skilled in the art can modify or change the above-mentioned embodiments without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and scope of the present invention as defined in the appended claims.
Claims (10)
1. A hemoglobin rapid detection device based on an intelligent mobile terminal is characterized by comprising a shell, a hemoglobin detection chip and the intelligent mobile terminal, wherein a light source and a battery are installed in the shell, and the battery is used for supplying power to the light source; the hemoglobin detection chip is provided with a reading area, the reading area is made of transparent materials, the reading area is provided with a micro-groove, a hemoglobin detection reagent is stored in the micro-groove, and a blood sample can enter the micro-groove through the siphoning action at the opening of the micro-groove and react with the hemoglobin detection reagent;
the shell is provided with a chip insertion hole and an intelligent mobile terminal placing groove, and the chip insertion hole is positioned above the intelligent mobile terminal placing groove; the intelligent mobile terminal is internally provided with an ambient light sensor, the light source is positioned above the chip insertion hole and used for emitting light to the reading area of the hemoglobin detection chip, and when the intelligent mobile terminal is placed in the placing groove, the light emitted by the light source is aligned with the ambient light sensor arranged in the intelligent mobile terminal;
an application program for reading and recording incident light and transmitted light intensity is installed in the intelligent mobile terminal, and the application program can calculate hemoglobin concentration according to a preset standard curve and display the hemoglobin concentration on a screen of the intelligent mobile terminal.
2. The hemoglobin rapid detection device based on intelligent mobile terminal of claim 1, characterized in that: the preparation method of the hemoglobin detection reagent in the microgrooves comprises the following steps: mixing a red blood cell lysate and a hemoglobin biochemical analysis reagent to prepare a hemoglobin detection solution, injecting the hemoglobin detection solution into a micro-tank, drying the hemoglobin detection solution by a vacuum freeze drying technology, and storing the dried hemoglobin detection solution in the micro-tank, wherein the red blood cell lysate is selected from any one of SDS, NP-40, tritonX-100 and sodium deoxycholate, and the hemoglobin biochemical analysis reagent is a detection reagent used in any one of an azide methemoglobin determination method, a bromohexadecyltrimethylamine hemoglobin determination method and an alkali-hydroxyhemoglobin determination method.
3. The hemoglobin rapid detection device based on the intelligent mobile terminal of claim 1, characterized in that: the micro-groove can contain 5-50 microliter of liquid, and the depth of the micro-groove is 50-100 micrometers.
4. The hemoglobin rapid detection device based on intelligent mobile terminal of claim 1, characterized in that: the transparent material is selected from any one of polymethyl methacrylate, polystyrene and polycarbonate.
5. The hemoglobin rapid detection device based on intelligent mobile terminal of claim 1, characterized in that: the hemoglobin detection chip is of a strip card type structure, one end of the chip is a reading area, and the other end of the chip is a handheld area.
6. The hemoglobin rapid detection device based on intelligent mobile terminal of claim 1, characterized in that: the light source is selected from any one of a laser, an LED lamp and a white light source processed by a grating and a light filter.
7. The hemoglobin rapid detection device based on the intelligent mobile terminal of claim 1, characterized in that: the housing is made of a material that is opaque to light.
8. The hemoglobin rapid detection device based on intelligent mobile terminal of claim 1, characterized in that: the shell is provided with a switch, and the switch is used for controlling the on and off of the light source.
9. The hemoglobin rapid detection device based on intelligent mobile terminal of claim 1, characterized in that: the intelligent mobile terminal is selected from any one of a smart phone, a tablet computer and a notebook computer.
10. A method for hemoglobin detection for non-disease diagnosis or treatment purposes, comprising: the hemoglobin concentration is measured using the device of any one of claims 1 to 9.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211107876.7A CN115639163A (en) | 2022-09-09 | 2022-09-09 | Hemoglobin rapid detection device based on intelligent mobile terminal and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211107876.7A CN115639163A (en) | 2022-09-09 | 2022-09-09 | Hemoglobin rapid detection device based on intelligent mobile terminal and application thereof |
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| CN115639163A true CN115639163A (en) | 2023-01-24 |
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| CN202211107876.7A Pending CN115639163A (en) | 2022-09-09 | 2022-09-09 | Hemoglobin rapid detection device based on intelligent mobile terminal and application thereof |
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