CN103901025A - Color detection device and method of portable paper-base micro fluidic chip - Google Patents
Color detection device and method of portable paper-base micro fluidic chip Download PDFInfo
- Publication number
- CN103901025A CN103901025A CN201210579935.0A CN201210579935A CN103901025A CN 103901025 A CN103901025 A CN 103901025A CN 201210579935 A CN201210579935 A CN 201210579935A CN 103901025 A CN103901025 A CN 103901025A
- Authority
- CN
- China
- Prior art keywords
- chip
- color detection
- detection device
- signal
- papery
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/77—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
- G01N21/78—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a change of colour
Abstract
The invention relates to a color detection device and method of a portable paper-base micro fluidic chip; the paper-base chip is placed in a chip card slot, the chip card slot is arranged in the device; a LED light source emitted by a light emitter irradiates a reaction region of the paper-base chip to reflect out reflected light; a color detection sensor is connected with a signal amplifying unit, is used for receiving the reflected light, and converts a light signal into an electrical signal to sent into the digital amplifier unit for signal amplification processing; a digital-to-analog conversion unit is connected with the signal amplifying unit and a programmable controller, is used for receiving an amplified digital electrical signal, and converts the digital electrical signal into an analog electrical signal to sent into the programmable controller for data processing. The color detection device is a new microfluidic device designed on the basis of the principle of light reflection on paper, is used for colorimetric analysis of biochemical reaction related color to provide quantitative analysis data, and can be widely used in the fields of family disease rapid diagnosis, on-site instant detection and the like.
Description
Technical field
The present invention relates to a kind of refill sheet technology of preparing, specifically a kind of portable paper substrate micro-fluidic chip color detection apparatus and method.
Background technology
Micro-fluidic refill chip technology is the study hotspot of current instrumental analysis, this technology is mainly take analytical chemistry and biological chemistry as basis, be applied to the various fields such as chemical analysis, environmental monitoring, immunoassay, medical diagnosis on disease, cell analysis, and sampling technique is as a guardian technique in micro-fluidic chip platform, a large amount of concerns of this area researchist are received.
At present, health care worker lacks the common disease that enough equipment is diagnosed.Therefore,, in the environment of resource-constrained, the development of low cost diagnostic techniques, makes suitable equipment portable and can load bearing of home medical applications demonstrate more and more important potentiality and status.These equipment volume are little, low price, and do not need very strong professional knowledge just can use, make unreachable health care originally become possibility.In traditional monitoring technology, the storage of sampling and transportation, cost is high, monitoring, higher technical ability and rich experience, be also faced with similar problem.Therefore, a kind of simple, fast, portable environmental monitoring technology is to win numerous awards and accolades.Micro-fluid experiment chamber chip is as promising and powerful platform, demonstrate huge potentiality, this technology is mainly take analytical chemistry and biological chemistry as basis, be applied to the various fields such as chemical analysis, environmental monitoring, immunoassay, medical diagnosis on disease, cell analysis, received a large amount of concerns of this area researchist.In one piece of nearest paper, the development of micro-fluidic chip technology is very rapidly according to these attractive functions, as low price, simple to operate, light and handy, transportation, and be easy to use.This is point of care diagnostic and the Real-Time Monitoring of field environment and the problem of environmental monitoring that is easy to touch, and promotes development.
At traditional test strip, result is inadequate often qualitatively so only to provide " Yes/No ".People need quantitative information more accurately.To the diagnosis of personal illness, a kind of mode is the checkout equipment that uses mobile phone, camera or scanner, and the paper after chemistry or biological respinse is taken pictures, and then converts obtained image to gray scale, and software is by the value calculating.But this method has obvious shortcoming, when data conversion treatment process is loaded down with trivial details, in family autodiagnosis, need checked people to there is good professional knowledge.The more important thing is, the light in environment can affect the Stability and veracity of detection.
Summary of the invention
For the deficiencies in the prior art, the invention provides a kind of portable paper substrate micro-fluidic chip color detection apparatus and method, it is microfluidic device design new on the basis of the light reflection principle of paper, the color that biochemical reaction is relevant is carried out to colorimetric analysis, the analysis data of quantification are provided, can be widely used in the field such as family disease quick diagnosis and on-the-spot instant detection.
The technical scheme that the present invention adopted is for achieving the above object: a kind of portable paper substrate micro-fluidic chip color detection device, comprises papery chip, core card groove, color detection sensor, optical transmitting set, digital amplifying unit, D/A conversion unit, Programmable Logic Controller; Described papery chip is placed in described core card groove, and core card groove is placed in described pick-up unit inside; The LED irradiation that described optical transmitting set sends is at the conversion zone of described papery chip; Described color detection sensor connects described signal amplification unit, for receiving the LED light of described papery chip reflection, and light signal is converted to and sends to described digital amplifying unit after electric signal and carry out signal and amplify and process; Described D/A conversion unit connects described signal amplification unit and described Programmable Logic Controller, for receiving the digital electric signal after amplification, and digital electric signal is converted to analog electrical signal sends to described Programmable Logic Controller and carries out data processing.
Described papery chip be filter paper or nitrocellulose paper make with the identical hard paper of core card groove shape.
Described core card groove comprises upper strata cover plate and bottom cover plate.
The conversion zone of described papery chip is the region that adds developer or biochemical reagents on papery chip, and this region is hydrophilic region.
Described upper strata cover plate and bottom cover plate are and core card groove shape is coincide lighttight hard material sheet, wherein arranges circular port in the conversion zone position of the corresponding papery chip of bottom cover plate, makes LED light source be irradiated to the conversion zone of papery chip.
Described optical transmitting set is light emitting diode.
Also comprise the USB interface for reading out data or charging.
Also comprise rechargeable battery, without external power supply in the situation that, provide electric power to device.
Described pick-up unit also comprises LCDs, connects described Programmable Logic Controller, is touch-screen, for the data that receive in real time and demonstration Programmable Logic Controller sends.
A kind of portable paper substrate micro-fluidic chip color detection method, comprises the following steps:
After splashing into testing liquid, the conversion zone of papery chip packs core card groove into, and by core card groove insertion apparatus inside;
The conversion zone back reflection that the LED light source that optical transmitting set sends is radiated at papery chip goes out reflected light;
Color detection sensor receives reflected light, and light signal is converted to and sends to digital amplifying unit after electric signal and carry out signal and amplify and process, D/A conversion unit receives the digital electric signal after amplification, and digital electric signal is converted to after analog electrical signal sends to Programmable Logic Controller and calculates and is exported in LCDs.
The present invention has following beneficial effect and advantage:
1. native system has been owing to having utilized totally enclosed test environment and stable LED light source, therefore detection signal is stable, and the interference of incidental surround lighting hardly;
2. native system device is of portable form, and reagent consumption is few, cheap, and more repeatably results can be provided.
Accompanying drawing explanation
Fig. 1 is hardware structure diagram of the present invention, wherein: 1, papery chip, 2, upper strata cover plate, 3, lower floor's cover plate, 4, optical transmitting set;
Fig. 2 is that this device is sample real-time quantitative detection collection of illustrative plates to albumen (BSA);
Fig. 3 is that this device is that sample real-time quantitative detects collection of illustrative plates to glucose;
Fig. 4 is that this device detects collection of illustrative plates take ferrous ion as sample real-time quantitative.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in further detail.
It mainly comprises a kind of paper substrate micro-fluidic chip color detection device of handheld portable and support chip: USB interface and the rechargeable battery of papery chip, core card groove, color detection sensor, optical transmitting set, digital amplifying unit, D/A conversion unit, Programmable Logic Controller, LCDs, exchanges data and charging.
Papery chip is produced to hydrophobic and hydrophilic region by printer wax spray or alternate manner, realize the pattern picture design of chip, in advance fixing developer or biochemical reagents in conversion zone, when dripping after sample, this region can produce color, on chip can there is chemistry or biological chromogenic reaction in the determinand of variable concentrations under the effect of its corresponding chromogenic reagent, the depth and the testing concentration of its chromogenic reaction are linear, this device detects this reaction by color detection sensor, and real-time quantitative analysis result can be provided.
The conversion zone of papery chip is hydrophilic region, and the region outside the conversion zone of papery chip is hydrophobic region, prevents the sample diffusion dripping.Papery chip is produced to hydrophobic and hydrophilic region by printer wax spray or alternate manner.
This device papery chip is inserted into device inside by core card groove, and whole testing environment has been totally enclosed, can not be subject to the impact of extraneous light and other condition.Adopt LED(light emitting diode) as detection light source (can have multiple), its illumination of launching is reflected after being mapped on papery chip, its intensity level can be by the quantitative perception of color detection sensor, then its numerical value 6 is shown in its intensity level in the LCDs that its device carries by controlled programmable device after by digital amplifying circuit and digital-to-analog conversion, and rechargeable battery part can be implemented real-time power supply to it.Whole testing environment has been totally enclosed, can not be subject to the impact of extraneous light and other condition.LED light source is real stable, so detection signal is stable.
The Programmable Logic Controller that this device is equipped with, can implement device in full-automatic collection and the processing of all data, thereby provide corresponding linear equation and can provide the quantitative result of unknown sample.
This device is equipped with USB interface, can reading out data and charging.
This paper substrate micro-fluidic chip color detection device, has that portability (hand-held), reagent consumption are low, with low cost, easy operating, it is characterized in that being mainly used in the field such as family disease quick diagnosis and on-the-spot instant detection.
This device and support chip thereof can carry out portability and quantitatively detect having the chemistry of color reaction and living things system (sample).
Embodiment 1: the making of micro-fluidic chip
Papery micro-fluidic chip is by papery chip (filter paper, nitrocellulose paper or other material) produce hydrophobic and hydrophilic region by printer wax spray or alternate manner, realize the pattern picture design of chip, in advance fixing developer or biochemical reagents in conversion zone, when dripping after sample, this region can produce color, the depth of color is relevant with test substance concentration, this refill sheet is inserted from card slot entrance, utilize the color detection sensor of its outfit to detect the reflected light of the conversion zone of refill sheet, can be convenient and swift, stablize effectively target substance is carried out qualitative and quantitatively detected.
Embodiment 2: the real-time quantitative of this device take albumen (BSA) as object detects research
In the reaction region to be measured of the micro-fluidic papery chip described in embodiment 1, add the citrate buffer solution (pH=1.8) of the 0.3M of 3 μ L, then add the bromophenol blue solution of 3 μ L in the above, after air drying, add 3 μ L bovine serum albumin(BSA) (BSA) solution to be measured until solution above at application of sample place, center.The papery chip color detection device that papery chip is inserted to portable (hand-held) formula, question response, after 2 minutes, reads record to measured value.Its quantitative result as shown in Figure 2, can carry out quantitative accurately to the bovine serum albumin(BSA) (BSA) within the scope of 5-50 μ M.The method utilizes the determinand of variable concentrations on chip that chemistry or biological chromogenic reaction can occur under the effect of its corresponding chromogenic reagent, and the depth and the testing concentration of its chromogenic reaction are linear.
Embodiment 3: the real-time quantitative of this device take glucose (glucose) as object detects research
In the reaction region to be measured of the micro-fluidic papery chip described in embodiment 1, drip the liquor kalii iodide (0.6M) of 3 μ L, then drip 3 horseradish peroxidases of μ L and the solution of glucose oxidase in the above, after solution is dry, drip the glucose (glucose) of 3 μ L in the above, papery chip is inserted to the papery chip color detection device of portable (hand-held) formula, after question response 2 minutes, measured value is read to record.Its quantitative result as shown in Figure 3, can carry out accurate quantitative analysis to the glucose (glucose) within the scope of 10-100 μ M.
Embodiment 4: the real-time quantitative that this device is object to divalent metal ferric ion detects research
Prepare acetate buffer, by sodium acetate trihydrate and glacial acetic acid constant volume, in 100 milliliters of pure water, its ultimate density is 6M(pH value=4.4).Contain 1 of 0.01M, the reagent of 10-phenanthroline by this buffer preparation.In the reaction region to be measured of the micro-fluidic papery chip described in embodiment 1, drip this 1,10-phenanthroline 3 μ L, and air is dry, drips the ferric ion sample of 3 μ L in the above, question response, after 2 minutes, reads record to measured value.As shown in Figure 4, can quantitatively detect ferric ion.
Claims (10)
1. a portable paper substrate micro-fluidic chip color detection device, is characterized in that: comprise papery chip, core card groove, color detection sensor, optical transmitting set, digital amplifying unit, D/A conversion unit, Programmable Logic Controller; Described papery chip is placed in described core card groove, and core card groove is placed in described pick-up unit inside; The LED irradiation that described optical transmitting set sends is at the conversion zone of described papery chip; Described color detection sensor connects described signal amplification unit, for receiving the LED light of described papery chip reflection, and light signal is converted to and sends to described digital amplifying unit after electric signal and carry out signal and amplify and process; Described D/A conversion unit connects described signal amplification unit and described Programmable Logic Controller, for receiving the digital electric signal after amplification, and digital electric signal is converted to analog electrical signal sends to described Programmable Logic Controller and carries out data processing.
2. the portable paper substrate micro-fluidic chip of one according to claim 1 color detection device, is characterized in that: described papery chip be filter paper or nitrocellulose paper make with the identical hard paper of core card groove shape.
3. the portable paper substrate micro-fluidic chip of one according to claim 1 and 2 color detection device, is characterized in that: described core card groove comprises upper strata cover plate and bottom cover plate.
4. the portable paper substrate micro-fluidic chip of one according to claim 1 color detection device, is characterized in that: the conversion zone of described papery chip is the region that adds developer or biochemical reagents on papery chip, and this region is hydrophilic region.
5. the portable paper substrate micro-fluidic chip of one according to claim 3 color detection device, it is characterized in that: described upper strata cover plate and bottom cover plate are and the identical lighttight hard material sheet of core card groove shape, wherein in the conversion zone position of the corresponding papery chip of bottom cover plate, circular port is set, makes LED light source be irradiated to the conversion zone of papery chip.
6. the portable paper substrate micro-fluidic chip of one according to claim 1 color detection device, is characterized in that: described optical transmitting set is light emitting diode.
7. the portable paper substrate micro-fluidic chip of one according to claim 1 color detection device, is characterized in that: also comprise the USB interface for reading out data or charging.
8. the portable paper substrate micro-fluidic chip of one according to claim 1 color detection device, is characterized in that: also comprise rechargeable battery, without external power supply in the situation that, provide electric power to device.
9. the portable paper substrate micro-fluidic chip of one according to claim 1 color detection device, it is characterized in that: described pick-up unit also comprises LCDs, connecting described Programmable Logic Controller, is touch-screen, for the data that receive in real time and demonstration Programmable Logic Controller sends.
10. a portable paper substrate micro-fluidic chip color detection method, is characterized in that, comprises the following steps:
After splashing into testing liquid, the conversion zone of papery chip packs core card groove into, and by core card groove insertion apparatus inside;
The conversion zone back reflection that the LED light source that optical transmitting set sends is radiated at papery chip goes out reflected light;
Color detection sensor receives reflected light, and light signal is converted to and sends to digital amplifying unit after electric signal and carry out signal and amplify and process, D/A conversion unit receives the digital electric signal after amplification, and digital electric signal is converted to after analog electrical signal sends to Programmable Logic Controller and calculates and is exported in LCDs.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210579935.0A CN103901025A (en) | 2012-12-27 | 2012-12-27 | Color detection device and method of portable paper-base micro fluidic chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210579935.0A CN103901025A (en) | 2012-12-27 | 2012-12-27 | Color detection device and method of portable paper-base micro fluidic chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103901025A true CN103901025A (en) | 2014-07-02 |
Family
ID=50992486
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210579935.0A Pending CN103901025A (en) | 2012-12-27 | 2012-12-27 | Color detection device and method of portable paper-base micro fluidic chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103901025A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104215758A (en) * | 2014-09-20 | 2014-12-17 | 桂林理工大学 | Instrument-free quantitative analysis method for fast detecting test paper, and application of instrument-free quantitative analysis method |
| CN105116041A (en) * | 2015-09-14 | 2015-12-02 | 青岛大学 | Portable microfluidic chip electrophoresis device and application thereof |
| CN107817244A (en) * | 2016-09-09 | 2018-03-20 | 美敦力公司 | Fluid sensor card |
| CN110320158A (en) * | 2018-03-30 | 2019-10-11 | 国际商业机器公司 | Mobile chemical analysis |
| CN111172025A (en) * | 2020-01-14 | 2020-05-19 | 中国科学院烟台海岸带研究所 | Compound micro-fluidic chip device of hydrogen sulfide activity detection based on cell |
| US11498069B2 (en) | 2020-08-31 | 2022-11-15 | International Business Machines Corporation | Rapid testing device for wastewater analysis |
| US11679186B2 (en) | 2016-09-09 | 2023-06-20 | Mozarc Medical Us Llc | Peritoneal dialysis fluid testing system |
| US11759557B2 (en) | 2011-04-29 | 2023-09-19 | Mozarc Medical Us Llc | Adaptive system for blood fluid removal |
| US11850344B2 (en) | 2021-08-11 | 2023-12-26 | Mozarc Medical Us Llc | Gas bubble sensor |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1566931A (en) * | 2003-06-23 | 2005-01-19 | 昆明云大生物技术有限公司 | Immunity detection and test paper scrip chroma quantitative determination instrument and detection method employed thereby |
| JP3738357B2 (en) * | 2002-08-05 | 2006-01-25 | 株式会社創成電子 | Portable sample analyzer |
| CN101614672A (en) * | 2009-07-21 | 2009-12-30 | 北京智云达科技有限公司 | A kind of portable extendible test paper analytical approach and equipment thereof |
| CN101769925A (en) * | 2009-12-22 | 2010-07-07 | 王继华 | Method and system for intelligently identifying and reading immunity-chromatography test strip and application thereof |
| CN101915832A (en) * | 2010-07-29 | 2010-12-15 | 陈晓汀 | Colloidal gold and double-flow enzyme-linked immunosorbent rapid detection all-in-one machine |
-
2012
- 2012-12-27 CN CN201210579935.0A patent/CN103901025A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3738357B2 (en) * | 2002-08-05 | 2006-01-25 | 株式会社創成電子 | Portable sample analyzer |
| CN1566931A (en) * | 2003-06-23 | 2005-01-19 | 昆明云大生物技术有限公司 | Immunity detection and test paper scrip chroma quantitative determination instrument and detection method employed thereby |
| CN101614672A (en) * | 2009-07-21 | 2009-12-30 | 北京智云达科技有限公司 | A kind of portable extendible test paper analytical approach and equipment thereof |
| CN101769925A (en) * | 2009-12-22 | 2010-07-07 | 王继华 | Method and system for intelligently identifying and reading immunity-chromatography test strip and application thereof |
| CN101915832A (en) * | 2010-07-29 | 2010-12-15 | 陈晓汀 | Colloidal gold and double-flow enzyme-linked immunosorbent rapid detection all-in-one machine |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11759557B2 (en) | 2011-04-29 | 2023-09-19 | Mozarc Medical Us Llc | Adaptive system for blood fluid removal |
| CN104215758A (en) * | 2014-09-20 | 2014-12-17 | 桂林理工大学 | Instrument-free quantitative analysis method for fast detecting test paper, and application of instrument-free quantitative analysis method |
| CN105116041A (en) * | 2015-09-14 | 2015-12-02 | 青岛大学 | Portable microfluidic chip electrophoresis device and application thereof |
| CN105116041B (en) * | 2015-09-14 | 2018-02-13 | 青岛大学 | A kind of portable micro-fluid control chip electrophoretic detection means and its application |
| CN107817244A (en) * | 2016-09-09 | 2018-03-20 | 美敦力公司 | Fluid sensor card |
| US11679186B2 (en) | 2016-09-09 | 2023-06-20 | Mozarc Medical Us Llc | Peritoneal dialysis fluid testing system |
| CN110320158A (en) * | 2018-03-30 | 2019-10-11 | 国际商业机器公司 | Mobile chemical analysis |
| US11060968B2 (en) | 2018-03-30 | 2021-07-13 | International Business Machines Corporation | Mobile chemical analysis |
| CN111172025A (en) * | 2020-01-14 | 2020-05-19 | 中国科学院烟台海岸带研究所 | Compound micro-fluidic chip device of hydrogen sulfide activity detection based on cell |
| CN111172025B (en) * | 2020-01-14 | 2022-11-15 | 中国科学院烟台海岸带研究所 | Compound micro-fluidic chip device of hydrogen sulfide activity detection based on cell |
| US11498069B2 (en) | 2020-08-31 | 2022-11-15 | International Business Machines Corporation | Rapid testing device for wastewater analysis |
| US11850344B2 (en) | 2021-08-11 | 2023-12-26 | Mozarc Medical Us Llc | Gas bubble sensor |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103901025A (en) | Color detection device and method of portable paper-base micro fluidic chip | |
| Chen et al. | Application of smartphone-based spectroscopy to biosample analysis: A review | |
| Kanchi et al. | Smartphone based bioanalytical and diagnosis applications: A review | |
| Liu et al. | Point-of-care testing based on smartphone: The current state-of-the-art (2017–2018) | |
| Yang et al. | Detection platforms for point-of-care testing based on colorimetric, luminescent and magnetic assays: A review | |
| US10921259B2 (en) | Method and apparatus for analyte measurement | |
| CN106062544B (en) | Quantification of assay changes | |
| US9901923B2 (en) | Mobile molecular diagnostics system with wireless communication | |
| Xu et al. | Advances in smartphone-based point-of-care diagnostics | |
| Kwon et al. | Medical diagnostics with mobile devices: Comparison of intrinsic and extrinsic sensing | |
| US9222875B2 (en) | Hand-held scanner systems and methods for reading point of care test results | |
| CN103901189B (en) | Immune detection micro-fluidic chip fluorescent quantitation automatic detection device | |
| CA2870648C (en) | Device for performing an enzyme-based diagnostic test and methods for use thereof | |
| US20130273563A1 (en) | Device for performing an enzyme-based diagnostic test and methods for use thereof | |
| Roda et al. | Smartphone-based biosensors for bioanalytics: A critical review | |
| US20220268758A1 (en) | Modular hand-held point of care testing system | |
| JP7184879B2 (en) | Smart pellets for sample testing | |
| CN110455786A (en) | One kind being based on CeO2@SnS2Promote the preparation method of luminol Electrochemiluminescsensor sensor | |
| CN110441295A (en) | One kind is based on ferritin encapsulation Ir (ppy)3Biosensor preparation method | |
| Boehle et al. | Single board computing system for automated colorimetric analysis on low-cost analytical devices | |
| JP2005257652A (en) | Detecting apparatus and analyzing method for biological sample | |
| CN115287176A (en) | Two-step isothermal nucleic acid amplification detection microfluidic device | |
| CN111190004B (en) | Instant detection system of immunochromatography test strip | |
| JP6011246B2 (en) | Fluorescence measurement method, fluorescence measurement kit, and fluorimeter | |
| Zhang et al. | The role played by sensors consisting of smartphone and black box in analytical chemistry: Increase the achievability |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20140702 |