CN111337683B - Portable HIV detection device - Google Patents

Portable HIV detection device Download PDF

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Publication number
CN111337683B
CN111337683B CN202010212883.8A CN202010212883A CN111337683B CN 111337683 B CN111337683 B CN 111337683B CN 202010212883 A CN202010212883 A CN 202010212883A CN 111337683 B CN111337683 B CN 111337683B
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China
Prior art keywords
hiv
side wall
clamping groove
portable
detection device
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Active
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CN202010212883.8A
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Chinese (zh)
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CN111337683A (en
Inventor
邱宪波
辛培
王东
张晓磊
乔杉
朱家君
李双双
杨殿龙
杨文博
董晓斌
李益民
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Beijing University of Chemical Technology
Beijing WanTai Biological Pharmacy Enterprise Co Ltd
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Beijing University of Chemical Technology
Beijing WanTai Biological Pharmacy Enterprise Co Ltd
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Priority to CN202010212883.8A priority Critical patent/CN111337683B/en
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/585—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with a particulate label, e.g. coloured latex
    • G01N33/587—Nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • G01N33/56988—HIV or HTLV
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/15—Retroviridae, e.g. bovine leukaemia virus, feline leukaemia virus, feline leukaemia virus, human T-cell leukaemia-lymphoma virus
    • G01N2333/155—Lentiviridae, e.g. visna-maedi virus, equine infectious virus, FIV, SIV
    • G01N2333/16—HIV-1, HIV-2

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Pathology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Virology (AREA)
  • Nanotechnology (AREA)
  • AIDS & HIV (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

The present disclosure relates to a portable HIV detection device, comprising: a detection box (1) with a hollow inner cavity; the clamping groove (2) is arranged in the hollow inner cavity and is configured to receive an HIV urine colloidal gold immunochromatographic strip (A) to be tested; the shooting unit (3) is arranged in the hollow inner cavity, is positioned at the opposite side of the clamping groove (2), and is configured to acquire an image of the HIV urine colloidal gold immunochromatography strip (A) in the clamping groove (2) and perform image processing; the controller is arranged in the hollow inner cavity, is in signal connection with the shooting unit (3), and is configured to receive image processing data provided by the shooting unit (3) and perform qualitative judgment; the hollow inner cavity can form a closed darkroom for the shooting unit (3) to collect images, and a paper strip inserting port (16) is arranged at the position, corresponding to the clamping groove (2), of the outer wall of the detection box (1). The embodiment of the disclosure has compact structure and occupies less space.

Description

Portable HIV detection device
Technical Field
The present disclosure relates to the field of medical detection, and in particular, to a portable human immunodeficiency virus (Human Immunodeficiency Virus, HIV for short) detection device.
Background
The current mainstream detection method of HIV comprises nucleic acid detection and immunodetection, wherein the nucleic acid detection is used for detecting HIV virus, and has high detection sensitivity; the immunodetection is used for detecting HIV antibodies, and the detection sensitivity is low, but the immunodetection is more convenient to implement. Whether nucleic acid or immunoassay, the test sample is primarily blood.
When a blood sample is used for HIV detection, besides the need for specialized personnel to complete blood drawing operations, a plurality of complicated operation steps are involved, and often the blood sample needs to be completed by specialized personnel through the operation of a plurality of detection instruments in specialized medical institutions such as hospitals. Thus, such HIV detection methods relying on blood as a detection sample are difficult to develop in a rapid environment (e.g., community or home) on site.
In order to improve the HIV detection efficiency and effectively control the epidemic of the acquired immunodeficiency syndrome (namely AIDS), the development of the HIV rapid detection method aiming at urine or saliva samples has important practical significance.
This facilitates the transfer of HIV detection from a medical facility to a non-medical environment as urine or saliva samples are obtained in a non-invasive manner. In some related art, rapid detection Of HIV urine test strips is achieved by lateral flow chromatography techniques on site Of Point-Of-Care Testing (POCT for short).
Disclosure of Invention
According to research, the equipment for detecting the HIV urine detection paper strip in the related technology occupies a large space, has high cost and is not beneficial to popularization and portable use of the detection device.
In view of the foregoing, embodiments of the present disclosure provide a portable HIV detection device that occupies less space.
In one aspect of the present disclosure, there is provided a portable HIV detection device comprising:
the detection box is provided with a hollow inner cavity;
the clamping groove is arranged in the hollow inner cavity and is configured to receive an HIV urine colloidal gold immunochromatographic strip to be tested;
the shooting unit is arranged in the hollow inner cavity, is positioned at the opposite side of the clamping groove and is configured to collect images of the HIV urine colloidal gold immunochromatographic strip in the clamping groove and perform image processing;
the controller is arranged in the hollow inner cavity, is in signal connection with the shooting unit and is configured to receive the image processing data provided by the shooting unit and perform qualitative judgment;
the hollow inner cavity can form a closed darkroom for the shooting unit to acquire images, and a paper strip inserting port is arranged at the position, corresponding to the clamping groove, of the outer wall of the detection box.
In some embodiments, the card slot is fixed on an inner side surface of a first side wall of the detection box, and the paper strip insertion slot is positioned on a second side wall of the detection box adjacent to the first side wall and connected with the first side wall.
In some embodiments, the card slot comprises a rectangular frame comprising:
the accommodating cavity is configured to accommodate the HIV urine colloidal gold immunochromatographic strip and limit the HIV urine colloidal gold immunochromatographic strip in the vertical direction of the first side wall;
a first opening disposed adjacent to the paper strip insertion opening and communicating between the accommodation chamber and the paper strip insertion opening; and
and the second opening is arranged towards the shooting unit and is communicated with the accommodating cavity along the vertical direction of the second side wall.
In some embodiments, the rectangular frame further comprises:
a third opening located at least one side of the accommodation chamber in a direction parallel to the first and second side walls and communicating with the accommodation chamber;
the clamping groove further comprises:
an elastic limiting piece, which is positioned at the third opening and is configured to limit the HIV urine colloidal gold immunochromatographic strip in the accommodating cavity in a direction parallel to the first side wall and the second side wall; and
the touch switch is connected with the elastic limiting piece and is in signal connection with the controller, and is configured to determine whether the HIV urine colloidal gold immunochromatographic strip is in place or not through movement of the elastic limiting piece, and send an in-place signal to the controller.
In some embodiments, the photographing unit includes:
the first circuit board is configured to process the acquired image through a pre-stored image processing algorithm;
the camera is arranged on the surface of the first circuit board adjacent to one side of the clamping groove, is connected with the controller through signals and is configured to collect images of the HIV urine colloidal gold immunochromatographic strip in the clamping groove; and
the backlight plate is provided with a mounting hole matched with the outline shape and the size of the lens of the camera, sleeved on the lens of the camera through the mounting hole and connected with the first circuit board and is configured to provide illumination light for the camera during image acquisition.
In some embodiments, the portable HIV detection device further includes a display screen, disposed on a side of the first circuit board away from the card slot, and in signal connection with the controller, configured to display a qualitative determination result of the HIV urine colloidal gold immunochromatographic strip;
the detection box is far away from the third side wall of draw-in groove one side be equipped with the through-hole of display screen shape and size matching, first circuit board with the display screen is all fixed to be set up the inboard surface of third side wall, the display screen is at least partly inlayed in the through-hole, and to the outside of third side wall exposes.
In some embodiments, the portable HIV detection device further comprises: a power supply module located within the hollow interior cavity configured to supply power to the photographing unit and the controller; the power supply module comprises a battery bin for accommodating a battery, and the battery bin is positioned on the same side of the clamping groove and the shooting unit and supports the surface, adjacent to one side of the clamping groove, of the first circuit board in a propping mode.
In some embodiments, the portable HIV detection device further comprises:
the fixing frame is fixedly arranged in the hollow inner cavity and is positioned at one side of the shooting unit far away from the battery compartment, and the fixing frame is configured to support the surface of the first circuit board, which is adjacent to one side of the clamping groove, together with the battery compartment in a propping way and limit the sliding of the backlight board.
In some embodiments, the controller comprises:
the singlechip control board is fixed on the inner side surface of a fourth side wall of the detection box through a plurality of connecting blocks, a preset gap is formed between the singlechip control board and the fourth side wall, and the fourth side wall is adjacent to a first side wall used for setting the clamping groove in the detection box and a third side wall used for setting the shooting unit;
in some embodiments, the portable HIV detection device further comprises:
the radio frequency identification module is arranged on the single chip microcomputer control board and is positioned between the single chip microcomputer control board and the inner side surface of the fourth side wall, the radio frequency identification module is electrically connected with the single chip microcomputer control board and is configured to read data in a radio frequency identification card of a person to be detected, so that the single chip microcomputer control board can conduct qualitative judgment according to the read data and image processing data provided by the shooting unit.
In some embodiments, the single chip microcomputer control board includes a bluetooth module configured to communicate with the intelligent mobile terminal over a bluetooth wireless link.
In some embodiments, the portable HIV detection device further comprises:
the fixing frame is fixedly arranged in the hollow inner cavity, is positioned at one side of the shooting unit, which is far away from the battery compartment, is connected with the single-chip microcomputer control board in a clamping mode, and is configured to support the surface, adjacent to one side of the clamping groove, of the first circuit board together with the battery compartment in a propping mode, and limit the backlight board and the single-chip microcomputer control board to slide.
In some embodiments, the power module further comprises a USB power line, the controller is configured to power the battery compartment when the USB power line is powered up, and to power the battery compartment when the USB power line is not powered up.
Therefore, according to the embodiment of the disclosure, the clamping groove for receiving the HIV urine colloidal gold immunochromatographic strip, the shooting unit for acquiring and analyzing the image of the strip in the clamping groove and the controller for performing qualitative judgment are arranged in the hollow inner cavity of the detection box, so that the clamping groove and the shooting unit are oppositely arranged in the closed dark room formed in the hollow inner cavity, and the strip inserting port communicated with the clamping groove is arranged on the outer wall of the detection box.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments of the disclosure and together with the description, serve to explain the principles of the disclosure.
The disclosure may be more clearly understood from the following detailed description taken in conjunction with the accompanying drawings in which:
FIG. 1 is a schematic external structural view of some embodiments of a portable HIV detection device according to the present disclosure;
FIG. 2 is a schematic diagram of an internal mounting structure of some embodiments of a portable HIV detection device according to the present disclosure;
FIG. 3 is an exploded schematic view of the internal structure of some embodiments of a portable HIV detection device according to the present disclosure;
FIG. 4 is a schematic diagram of the mounting structure of a card slot in some embodiments of a portable HIV detection device according to the present disclosure;
FIG. 5 is a schematic view of a portion of the structure of a camera unit in some embodiments of a portable HIV detection device according to the present disclosure;
fig. 6 is a schematic structural view of a backlight plate in some embodiments of a portable HIV detection device according to the present disclosure.
It should be understood that the dimensions of the various elements shown in the figures are not drawn to actual scale. Further, the same or similar reference numerals denote the same or similar members.
Detailed Description
Various exemplary embodiments of the present disclosure will now be described in detail with reference to the accompanying drawings. The description of the exemplary embodiments is merely illustrative, and is in no way intended to limit the disclosure, its application, or uses. The present disclosure may be embodied in many different forms and is not limited to the embodiments described herein. These embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art. It should be noted that: the relative arrangement of parts and steps, the composition of materials, numerical expressions and numerical values set forth in these embodiments should be construed as exemplary only and not limiting unless otherwise specifically stated.
The terms "first," "second," and the like, as used in this disclosure, do not denote any order, quantity, or importance, but rather are used to distinguish one element from another. The word "comprising" or "comprises" and the like means that elements preceding the word encompass the elements recited after the word, and not exclude the possibility of also encompassing other elements. "upper", "lower", "left", "right", etc. are used merely to indicate relative positional relationships, which may also be changed when the absolute position of the object to be described is changed.
In this disclosure, when a particular device is described as being located between a first device and a second device, there may or may not be an intervening device between the particular device and either the first device or the second device. When it is described that a particular device is connected to other devices, the particular device may be directly connected to the other devices without intervening devices, or may be directly connected to the other devices without intervening devices.
All terms (including technical or scientific terms) used in this disclosure have the same meaning as commonly understood by one of ordinary skill in the art to which this disclosure belongs, unless specifically defined otherwise. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the relevant art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.
Techniques, methods, and apparatus known to one of ordinary skill in the relevant art may not be discussed in detail, but are intended to be part of the specification where appropriate.
As shown in fig. 1, is a schematic diagram of the external structure of some embodiments of a portable HIV detection device according to the present disclosure. Referring to fig. 1, in combination with fig. 2-6, in some embodiments, a portable HIV detection device includes: a detection box 1, a clamping groove 2, a shooting unit 3 and a controller. The cartridge 1 has a hollow interior. The clamping groove 2 is arranged in the hollow inner cavity and is configured to receive the HIV urine colloidal gold immunochromatographic strip A to be tested. The shooting unit 3 is arranged in the hollow inner cavity, is positioned at the opposite side of the clamping groove 2, and is configured to collect and process images of the HIV urine colloidal gold immunochromatography strip A in the clamping groove 2. The photographing unit 3 may acquire the gray values of the detection line, the control line, and the background area of the strip a through a self-contained image processing algorithm, and provide these data as raw detection data to the controller.
The controller is arranged in the hollow inner cavity, is in signal connection with the shooting unit 3, and is configured to receive the image processing data provided by the shooting unit 3 and perform qualitative judgment based on a lateral flow analysis technology. The controller may implement a qualitative determination process using existing lateral flow chromatography techniques, which are not described here in detail, given that lateral flow chromatography techniques are not part of the present disclosure. The hollow inner cavity can form a closed darkroom for the shooting unit 3 to collect images, and a paper strip inserting port 16 is arranged at the position of the outer wall of the detection box 1 corresponding to the clamping groove 2.
According to the embodiment, the clamping groove for receiving the HIV urine colloidal gold immunochromatographic strip, the shooting unit for collecting and analyzing the image of the strip in the clamping groove and the controller for qualitative judgment are arranged in the hollow inner cavity of the detection box, so that the clamping groove and the shooting unit are oppositely arranged in the closed darkroom formed in the hollow inner cavity, the strip inserting port communicated with the clamping groove is arranged on the outer wall of the detection box, and the clamping groove, the shooting unit and the controller are arranged in the compact space of the detection box, so that the size of the device is effectively reduced, the occupied space is reduced, and the portability is improved.
Referring to fig. 1 and 2, in some embodiments, the cartridge 1 may be a square or rectangular box made of an opaque material, such as plastic or metal. The hollow inner cavity of the detection box 1 can form a closed darkroom to prevent light outside the detection box 1 from entering the hollow inner cavity, thereby preventing light leakage from adversely affecting the image acquisition of the shooting unit 3.
In fig. 2, the card slot 2 is fixed on the inner surface of the first side wall 11 of the detection box 1, and the paper strip insertion slot 16 is located on the second side wall 12 of the detection box 1 adjacent to the first side wall 11 and connected with the first side wall 11. Thus, an operator can conveniently insert the HIV urine colloidal gold immunochromatographic strip A from the strip inserting port 16 into the clamping groove 2 directly, and the position for effective image acquisition is reached. And the draw-in groove is fixed at the inboard surface of first lateral wall 11 for the male HIV urine colloidal gold immunochromatographic strip A can support under the spacing effect of draw-in groove on the inboard surface of first lateral wall 11, thereby can keep the position stability of strip when image acquisition, avoid the strip to become flexible in the draw-in groove and influence image acquisition effect.
Referring to fig. 3 and 4, in some embodiments, the card slot 2 includes a rectangular frame 21. The rectangular frame 21 includes: a receiving cavity, a first opening 22 and a second opening 23. The accommodating cavity is configured to accommodate the HIV urine colloidal gold immunochromatographic strip a and limit the HIV urine colloidal gold immunochromatographic strip a in a vertical direction of the first side wall 11. The first opening 22 is disposed adjacent to the paper strip insertion opening 16 and communicates between the receiving chamber and the paper strip insertion opening 16. The second opening 23 is provided toward the photographing unit 3 and communicates with the accommodating chamber in a vertical direction of the second sidewall 12. So that the photographing unit 3 can clearly photograph the image of the HIV urine colloidal gold immunochromatographic strip a in the accommodation chamber through the second opening.
In addition to the limiting effect on the HIV urine colloidal gold immunochromatographic strip a in the vertical direction of the first side wall 11, in order to prevent the HIV urine colloidal gold immunochromatographic strip a from loosening in other directions, referring to fig. 3 and 4, in some embodiments, the card slot 2 further includes an elastic limiting member 24. The elastic stopper 24 is configured to stop the HIV-urine colloidal gold immunochromatographic strip a in the accommodation chamber in a direction parallel to the first side wall 11 and the second side wall 12.
In fig. 3 and 4, the rectangular frame 21 further includes: and a third opening which is located at least one side of the accommodation chamber in a direction parallel to the first side wall 11 and the second side wall 12 and communicates with the accommodation chamber. The elastic limiting member 24 can be disposed at the third opening, so as to prevent the HIV urine colloidal gold immunochromatographic strip a from interfering with the entry and exit of the HIV urine colloidal gold immunochromatographic strip a, and can apply lateral extrusion force after the HIV urine colloidal gold immunochromatographic strip a enters, so as to prevent the HIV urine colloidal gold immunochromatographic strip a from shaking or falling out. In some embodiments, the rectangular frame 21 has two opposite third openings, and an elastic limiting member is disposed in each third opening, so as to obtain a more stable paper strip limiting effect.
Referring to fig. 4, in some embodiments, the card slot 2 may further include a touch switch 25. The touch switch 25 may be connected to the elastic limiting member 24 and is in signal connection with the controller. It can determine whether the HIV urine colloidal gold immunochromatographic strip A is in place by the movement of the elastic limiting member 24, and send an in-place signal to the controller. Therefore, the controller can timely know the in-place condition of the HIV urine colloidal gold immunochromatographic strip A, so as to determine whether test preparation is finished.
In fig. 4, the elastic limiting member 24 may include a metal sheet bent at an angle (for example, 120 ° to 150 °), one end may be fixed to the outside of the rectangular frame 21, and the other end may be close to or abut against the touch switch 25 at a side away from the rectangular frame 21. The bent part between the two ends of the elastic limiting piece 24 enters the third opening, and can be abutted against the side edge of the HIV urine colloidal gold immunochromatographic strip A when the strip A is inserted into the accommodating cavity.
Referring to fig. 2 to 3 and 5 to 6, in some embodiments, the photographing unit 3 includes: a first circuit board 31, a camera head 32, and a backlight 34. The first circuit board 31, the camera head 32 and the backlight 34 are compactly integrated together, so that less space can be occupied, and power consumption is lower, thereby having longer working time.
The camera 32 is disposed on a surface of the first circuit board 31 adjacent to one side of the card slot 2, and is connected with the controller through a signal, and is configured to collect images of the HIV urine colloidal gold immunochromatographic strip a in the card slot 2. The first circuit board 31 can process the acquired image by a pre-stored image processing algorithm. The relative position of the camera 32 and the clamping groove 2 is fixed, and when the camera 32 is installed, the focal length of the camera 32 can be set to be a numerical value capable of clearly shooting the surface of the paper strip.
The backlight 34 may employ a uniform green or white light source. In some embodiments, the backlight 34 specifically includes an LED light source, a circuit board, a light guide plate, a reflective film, a diffusion film, a light shielding film, and the like. Light emitted from the LED light source is emitted to the direction in which the card slot 2 is located via the light guide plate and the diffusion film. The backlight 34 can provide illumination to the camera head 32 during image acquisition so that a clearer image is acquired by the camera head 32.
In fig. 3, the backlight 34 has a mounting hole 34a that matches the lens outline shape and size of the camera head 32. The backlight plate 34 may be sleeved on the lens of the camera 32 through the mounting hole 34a, and may be connected to the first circuit board 31 through a wire 34 b. The backlight plate 34 sleeved on the lens can be closer to the surface of the detected paper strip, so that shadows generated in the illumination process can be effectively eliminated, and the detection effect is ensured.
Referring to fig. 2 and 3, in some embodiments, the portable HIV detection device further includes: and a display screen 8. The display 8 may be a liquid crystal display, an organic light emitting diode display, or the like. The display screen 8 is disposed on a side of the first circuit board 31 away from the card slot 2, and is in signal connection with the controller, and configured to display a qualitative judgment result of the HIV urine colloidal gold immunochromatographic strip a.
In fig. 1 and 3, the third side wall 13 of the cartridge 1 on the side remote from the card slot 2 is provided with a through hole 15 matching the shape and size of the display screen 8. The first circuit board 31 and the display screen 8 are fixedly disposed on the inner side surface of the third side wall 13, and the display screen 8 is at least partially embedded in the through hole 15 and is exposed to the outside of the third side wall 13. Since the display 8 itself is thin and it is attached to the third side wall 13 and partially embedded in the through hole 15, it occupies only a small amount of space inside the cartridge 1 in its thickness direction. In addition, the display screen 8 can also form an abutting support in surface contact with the first circuit board 31 of the photographing unit 3, and the stability of the installation of the display screen 8 is improved.
Referring to fig. 2 and 3, in some embodiments, the portable HIV detection device further includes: a power supply module 4 positioned in the hollow inner cavity. The power supply module 4 is capable of supplying power to the photographing unit 3 and the controller. To improve portability, the power supply module 4 may include a battery compartment 41 for accommodating a battery. The battery compartment 41 may be disposed on the same side of the card slot 2 and the photographing unit 3, and may support the surface of the first circuit board 31 adjacent to the card slot 2. A cover plate 42 may be provided on the outside of the battery compartment 41 to effect closure of the battery compartment 41, and a replaceable battery 43 (e.g., an alkaline battery) or a rechargeable battery (e.g., a lithium battery) may be provided inside the battery compartment 41.
In other embodiments, the power module 4 may further include other power lines, such as a USB power line, to implement dual or multiple power management functions. The controller may stop the power supply to the battery compartment 41 when the USB power supply line is connected, and may supply the power to the battery compartment 41 when the USB power supply line is not connected. Thus, the portable HIV detection device can be more flexible to use and is suitable for various different power supply environments.
In fig. 2, the portable HIV detection device may further include: and the fixing frame 7 is fixedly arranged in the hollow inner cavity. The fixing frame 7 may be located at a side of the photographing unit 3 away from the battery compartment 41, and is configured to support the surface of the first circuit board 31 adjacent to the side of the card slot 2 together with the battery compartment 41, and limit the sliding of the backlight board 34 together with the battery compartment 41. In this way, the battery compartment 41 and the fixing frame 7 are fully utilized to effectively fix the components in the shooting unit 3, and the internal space occupied by the special fixing structure of the shooting unit 3 is omitted.
Referring to fig. 2 and 3, in some embodiments, the controller includes: and a single chip microcomputer control board 51. The single chip microcomputer control board 51 may be fixed on the inner side surface of the fourth side wall 14 of the detection box 1 through a plurality of connection blocks, and a preset gap is formed between the single chip microcomputer control board and the fourth side wall 14. In fig. 2, the four corners of the single-chip microcomputer control board 51 may be provided with connection blocks 52a, 52b, 52c, and 52d, respectively. Four screw holes 17 are correspondingly arranged on the fourth side wall 14 at positions corresponding to the connecting blocks, and the singlechip control board 51 can be reliably fixed on the inner side surface of the fourth side wall 14 through connecting pieces such as screws. The fourth side wall 14 here adjoins both the first side wall 11 and the third side wall 13.
Referring to fig. 2, in some embodiments, the portable HIV detection device further includes a radio frequency identification (Radio Frequency Identification, RFID for short) module 6. The RFID module 6 may be disposed on the single chip microcomputer control board 51 and located between the single chip microcomputer control board 51 and the inner side surface of the fourth side wall 14. The RFID module 6 is disposed between the single-chip microcomputer control board 51 and the fourth side wall 14, occupies less space in the vertical direction of the fourth side wall 14, and is closer to the fourth side wall 14, so that signals are more sensitive when the RFID card is identified.
The RFID module 6 is electrically connected to the single-chip microcomputer control board 51, and is configured to read data in the RFID card of the subject, so that the single-chip microcomputer control board 51 performs qualitative judgment according to the read data and the image acquired by the shooting unit 3. For example, different detection standards may be required for different types and batches of test strips. Accordingly, the RFID module 6 can read the data of the RFID card to determine the proper detection standard so as to meet the detection requirements of different types of paper strips to be detected.
In fig. 3, the fixing frame may also be clamped with the single-chip microcomputer control board 51. The fixing frame can be provided with a groove at one side adjacent to the fourth side wall 14 for clamping the end part of the singlechip control board 51 adjacent to one side of the shooting unit, so that at least partial overlapping of the fixing frame and the singlechip control board 51 in the thickness direction can be realized, and the size of the internal space is further reduced. The fixing frame 7 and the battery compartment 41 can support the surface of the first circuit board 31 adjacent to the side of the card slot 2 together, and limit the backlight 34 and the single-chip microcomputer control board 51 from sliding.
In some embodiments, the single-chip microcomputer control board 51 includes a bluetooth module configured to communicate with the intelligent mobile terminal via a bluetooth wireless link. When the singlechip control board 51 obtains the detection result of qualitative judgment according to the image acquired by the shooting unit 3, the detection result can be transmitted to an intelligent mobile terminal (such as a smart phone) in real time through a Bluetooth module and further transmitted to a remote medical monitoring center according to the requirement.
In fig. 1, a button 18a and a button 18b may be provided on the outside of the cartridge 1. Button 18a may be used for power on and off of the device and button 18b may be used to detect the start of the process. After the button 18b is pressed, the singlechip control board 51 can issue a control instruction to the photographing unit 3 to control the camera to take a photograph. The singlechip control board 51 can display the qualitative detection result through the liquid crystal screen 8 through image processing and analysis and judgment, and send the qualitative detection result to the intelligent mobile terminal through the Bluetooth module.
With reference to fig. 1-6 and the various embodiments described above, the operation of some embodiments of the portable HIV detection device will be described.
The operator can first open the button 18a and bring the RFID card close to the RFID detection area (i.e. the fourth side wall 14), read the information stored in the RFID card by means of the RFID module and display it on the display screen 8. Next, the operator can insert the fully reacted HIV urine colloidal gold immunochromatographic strip a into the card slot 2 via the strip insertion slot 16. When the paper strip is inserted in place, the touch switch 25 can send an in-place signal to the singlechip control board 51. The singlechip control board 51 can display prompt messages such as 'paper fully inserted' through a display screen so as to indicate that the preparation work before the test is finished at the moment.
The operator may press button 18b to initiate the detection process. In the detection process, the camera 32 collects the image information of the HIV urine colloidal gold immunochromatographic strip a, then obtains raw data such as gray values of a strip detection line, a control line and a background area through an image processing algorithm of a driving chip in the camera module, and sends the data to the singlechip control board 51. The singlechip control board 51 performs qualitative judgment according to the data, and displays the detection result of the qualitative judgment through the display screen 8, and simultaneously transmits the detection result to the smart phone of the operator through the Bluetooth module so that the operator can check the detection result through the smart phone. The process of detecting a plurality of strips may be performed by repeating the above steps.
By way of illustration of the above-described embodiments of the portable HIV detection device of the present disclosure, at least one of the above-described embodiments of the present disclosure has the following advantages:
1. the portable HIV detection device can realize the rapid qualitative detection of HIV by combining the HIV colloidal gold immunochromatography strip with a urine sample, thereby providing a non-invasive HIV rapid detection matching instrument for the HIV detection prevention and control of basic medical institutions, communities and even families;
2. for operators, the portable HIV detection device can realize convenient detection operation through fewer keys, and professional training is not required for the operators;
3. the portable HIV detection device is compact in structural design, greatly reduces the size of the device, supports handheld use, and remarkably reduces the instrument cost while ensuring the detection effect through the integration of an optimized optical-mechanical-electrical integrated system;
4. the detection result of the portable HIV detection device can be displayed through the display screen of the detection box, the detection result transmitted through the Bluetooth module can be checked through the smart phone, and the detection structure can be further transmitted to a specialized medical monitoring data center, so that efficient real-time HIV prevention and control monitoring is realized.
Thus, various embodiments of the present disclosure have been described in detail. In order to avoid obscuring the concepts of the present disclosure, some details known in the art are not described. How to implement the solutions disclosed herein will be fully apparent to those skilled in the art from the above description.
Although some specific embodiments of the present disclosure have been described in detail by way of example, it should be understood by those skilled in the art that the above examples are for illustration only and are not intended to limit the scope of the present disclosure. It will be understood by those skilled in the art that the foregoing embodiments may be modified and equivalents substituted for elements thereof without departing from the scope and spirit of the disclosure. The scope of the present disclosure is defined by the appended claims.

Claims (10)

1. A portable HIV detection device, comprising:
a detection box (1) with a hollow inner cavity;
the clamping groove (2) is arranged in the hollow inner cavity and is configured to receive an HIV urine colloidal gold immunochromatographic strip (A) to be tested;
the shooting unit (3) is arranged in the hollow inner cavity, is positioned at the opposite side of the clamping groove (2), and is configured to acquire an image of the HIV urine colloidal gold immunochromatography strip (A) in the clamping groove (2) and perform image processing;
the controller is arranged in the hollow inner cavity, is in signal connection with the shooting unit (3), and is configured to receive image processing data provided by the shooting unit (3) and perform qualitative judgment;
the hollow inner cavity can form a closed darkroom for the shooting unit (3) to collect images, and a paper strip inserting port (16) is arranged at the position of the outer wall of the detection box (1) corresponding to the clamping groove (2);
wherein the photographing unit (3) includes:
a first circuit board (31) configured to process the acquired image by a pre-stored image processing algorithm;
the camera (32) is arranged on the surface of the first circuit board (31) adjacent to one side of the clamping groove (2), is connected with the controller through signals and is configured to collect images of HIV urine colloidal gold immunochromatography strips (A) in the clamping groove (2); and
a backlight plate (34) having a mounting hole (34 a) matching the outer contour shape and size of the lens of the camera (32), the backlight plate (34) being sleeved on the lens of the camera (32) through the mounting hole (34 a) and connected with the first circuit board (31) and configured to provide illumination light to the camera (32) during image acquisition;
wherein the portable HIV detection device further comprises:
a power supply module (4) located within the hollow interior cavity and configured to supply power to the shooting unit (3) and the controller; wherein the power supply module (4) comprises a battery compartment (41) for accommodating a battery; and
the fixing frame (7) is fixedly arranged in the hollow inner cavity and is positioned at one side of the shooting unit (3) far away from the battery compartment (41), and is configured to support the surface of the first circuit board (31) adjacent to one side of the clamping groove (2) together with the battery compartment (41) in a propping mode and limit the backlight board (34) to slide.
2. The portable HIV detection device according to claim 1, wherein the card slot (2) is fixed to an inner side surface of a first side wall (11) of the detection case (1), and the paper strip insertion slot (16) is located on a second side wall (12) of the detection case (1) adjacent to the first side wall (11) and in a portion connected to the first side wall (11).
3. Portable HIV detection device according to claim 2, characterized in that the card slot (2) comprises a rectangular frame (21), the rectangular frame (21) comprising:
the accommodating cavity is configured to accommodate the HIV urine colloidal gold immunochromatographic strip (A) and limit the HIV urine colloidal gold immunochromatographic strip (A) in the vertical direction of the first side wall (11);
a first opening (22) disposed adjacent to the paper strip insertion opening (16) and communicating between the accommodation chamber and the paper strip insertion opening (16); and
and a second opening (23) which is provided toward the photographing unit (3) and communicates with the accommodation chamber in a vertical direction of the second side wall (12).
4. A portable HIV detection device according to claim 3, wherein the rectangular frame (21) further comprises:
a third opening located on at least one side of the accommodation chamber in a direction parallel to the first side wall (11) and the second side wall (12), and communicating with the accommodation chamber;
the clamping groove (2) further comprises:
an elastic limiting piece (24) is positioned at the third opening and is configured to limit the HIV urine colloidal gold immunochromatographic strip (A) in the containing cavity in a direction parallel to the first side wall (11) and the second side wall (12); and
the touch switch (25) is connected with the elastic limiting piece (24) and is in signal connection with the controller, and is configured to determine whether the HIV urine colloidal gold immunochromatographic strip (A) is in place or not through the movement of the elastic limiting piece (24) and send an in-place signal to the controller.
5. The portable HIV detection device of claim 1, further comprising: the display screen (8) is arranged on one side, far away from the clamping groove (2), of the first circuit board (31), is connected with the controller through signals and is configured to display a qualitative judgment result of the HIV urine colloidal gold immunochromatography strip (A);
the detection box (1) is far away from a third side wall (13) on one side of the clamping groove (2) and is provided with a through hole (15) matched with the display screen (8) in shape and size, the first circuit board (31) and the display screen (8) are fixedly arranged on the inner side surface of the third side wall (13), and the display screen (8) is at least partially embedded in the through hole (15) and is exposed to the outer side of the third side wall (13).
6. The portable HIV detection device of claim 1, wherein the controller includes:
the single chip microcomputer control board (51) is fixed on the inner side surface of a fourth side wall (14) of the detection box (1) through a plurality of connecting blocks, a preset gap is formed between the fourth side wall and the fourth side wall (14), and the fourth side wall (14) is adjacent to a first side wall (11) used for setting the clamping groove (2) and a third side wall (13) used for setting the shooting unit (3) in the detection box (1).
7. The portable HIV detection device of claim 6, further comprising:
the radio frequency identification module (6) is arranged on the single chip microcomputer control board (51) and is positioned between the single chip microcomputer control board (51) and the inner side surface of the fourth side wall (14), the radio frequency identification module (6) is electrically connected with the single chip microcomputer control board (51) and is configured to read data in a radio frequency identification card of a person to be detected, so that the single chip microcomputer control board (51) can conduct qualitative judgment according to the read data and image processing data provided by the shooting unit (3).
8. The portable HIV detection device according to claim 6, wherein the single chip microcomputer control board (51) includes a bluetooth module configured to communicate with the intelligent mobile terminal over a bluetooth wireless link.
9. The portable HIV detection device according to claim 6, wherein the mount (7) is clamped to the single-chip microcomputer control board (51) and is configured to limit sliding of the backlight board (34) and the single-chip microcomputer control board (51).
10. The portable HIV detection device according to claim 1, wherein the power module (4) further comprises a USB power line, the controller being configured to deactivate the battery compartment (41) when the USB power line is powered up and to deactivate the battery compartment (41) when the USB power line is not powered up.
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