CN115634726A

CN115634726A - Pathological tissue manufacturing equipment

Info

Publication number: CN115634726A
Application number: CN202211660157.8A
Authority: CN
Inventors: 请求不公布姓名
Original assignee: Tianjin Mingrui Pharmaceutical Technology Co ltd
Current assignee: Tianjin Mingrui Pharmaceutical Technology Co ltd
Priority date: 2022-12-23
Filing date: 2022-12-23
Publication date: 2023-01-24
Anticipated expiration: 2042-12-23
Also published as: CN115634726B

Abstract

The invention discloses a pathological tissue preparation device, which is characterized by comprising: a tissue chamber in which test tubes are disposed; a first reagent bin in which a first reagent bottle is disposed; a first conduit connecting the first reagent bottle to the test tube in the tissue cartridge; a first pump disposed on the first conduit for delivering reagent in the first reagent bottle into the test tube; an air duct, one end of which is connected with the test tube and the other end of which is communicated with the outside air of the test tube; and a control device electrically connected to the first pump.

Description

Pathological tissue manufacturing equipment

Technical Field

The application relates to a pathological tissue manufacturing device, in particular to a thermostat for manufacturing pathological tissues.

Background

The pathological tissue preparation technology is widely applied to medical clinics and scientific researches. The pathological tissue is usually prepared by dehydrating, transparentizing, and waxing a tissue specimen to prepare a tissue wax block. Hospitals are required to detect large amounts of pathological tissue each day. The time and quality of pathological tissue preparation are related to temperature, type of pathological tissue and size of pathological tissue. Tissue preparation has temperature requirements, and the quality and safety of tabletting can be affected by overhigh or overlow temperature.

Currently, pathological tissue preparation is usually performed in a large-capacity device. In this apparatus, the pathological tissues are produced in a batch at the same time, there is a lack of flexibility, and the space occupied by the production of the pathological tissues is too large. Moreover, regardless of the number of pathological tissues to be prepared, a large amount of reagents are required to be used and consumed in one experiment, resulting in high preparation cost of pathological specimens. In addition, pathological tissues of different types and sizes are placed in the same experimental environment for pathological tissue preparation, so that the quality of pathological specimens cannot be accurately controlled. The pathological tissue manufacturing process is basically semi-automatic, and still needs to be carried out manually, thereby occupying a large amount of manpower and time and having lower pathological tissue manufacturing efficiency. Moreover, the number of pathological examinations increases year by year, and higher requirements are put on the number and time of tissue production. Therefore, it is urgently needed to improve the flexibility, automation degree, production efficiency and production quality of pathological tissue production.

Disclosure of Invention

In order to solve the problems of the prior art, the present invention provides a pathological tissue preparation apparatus, comprising: a tissue chamber in which test tubes are disposed; a first reagent bin in which a first reagent bottle is disposed; a first conduit connecting the first reagent bottle to the test tube in the tissue cartridge; a first pump disposed on the first conduit for delivering reagent in the first reagent bottle into the test tube; an air duct, one end of which is connected with the test tube and the other end of which is communicated with the outside air of the test tube; and a control device electrically connected to the first pump.

In particular, the pathological tissue preparation device is an incubator. Preferably, the first conduit is connected to the test tube at the bottom of the test tube and/or the vent conduit is connected to the test tube at the top of the test tube.

Preferably, one or more tube sets are provided in the tissue cartridge, each tube set comprising one tube or a plurality of tubes connected in series, wherein the first conduit has at one end thereof one or more first tube-end branch conduits connected respectively to the respective one or more tube sets.

Preferably, the pathological tissue preparation apparatus further comprises a first reagent buffer bottle connected to the one or more tube sets by a first top conduit, wherein the first top conduit has at one end remote from the first reagent buffer bottle a respective one or more first top branch conduits connected to the one or more tube sets, respectively. Advantageously, the first reagent buffer bottle is located in the tissue magazine or the first reagent magazine. The first reagent buffer bottle is configured to draw reagent from the test tube and buffer the reagent therein, for example, by a pump, or to push the reagent buffered therein into the test tube, thereby achieving recycling of the reagent. Preferably, the first reagent buffer bottle can be located on the air vent line. That is, the first top conduit is a section of the vent conduit and the first reagent buffer bottle may be formed by thickening a portion of the vent conduit. At this point, the exhaust duct also serves to buffer the reagent and only occupies one port at the top of the test tube (when the exhaust duct is a separate component from the first reagent buffer bottle, both are connected to the test tube through two ports).

Advantageously, said airway tube is selected from a group having a respective one or more airway branch tubes connected respectively to said one or more tube sets. Alternatively, the pathological tissue preparation apparatus has respective one or more airway tubes connected to the one or more tube sets, respectively.

In a preferred embodiment, a plurality of test tubes are disposed in the tissue cartridge, and the first conduit has a plurality of first test tube end branch conduits connected to the plurality of test tubes, respectively, at one end thereof. Preferably, the airway tube has a plurality of airway branch tubes at one end of the tissue cartridge connected to the plurality of test tubes, respectively. Alternatively, the pathological tissue preparation apparatus has a plurality of airway tubes connected to the plurality of test tubes, respectively.

In one variant, a plurality of tube sets, each comprising one tube or a plurality of tubes connected in series, are provided in the tissue magazine, wherein the first conduit has, at one end located in the tissue magazine, a plurality of first tube-end branch conduits respectively connected to the plurality of tube sets. Preferably, the airway tube has a plurality of airway branch tubes at one end of the tissue cartridge connected to the plurality of tube sets, respectively. Alternatively, the pathological tissue preparation apparatus has a plurality of airway tubes connected to the plurality of tube groups, respectively.

In particular, when the test tube group comprises a plurality of test tubes connected in series, said plurality of test tubes are arranged in the tissue magazine in an up-down direction. Preferably, the first tube-end branch duct is connected to the bottommost duct of the plurality of tubes arranged one above the other and/or the vent branch duct or the vent duct is connected to the topmost duct of the plurality of tubes arranged one above the other.

In one embodiment, a plurality of first reagent bottles are disposed in the first reagent magazine, and the first conduit has a plurality of first reagent end branch conduits at one end of the first reagent magazine connected to the plurality of first reagent bottles, respectively.

In one embodiment, a plurality of first reagent bottles are provided in the first reagent magazine, and the pathological tissue preparation apparatus has a plurality of first conduits connected from the test tubes in the tissue magazine to the plurality of first reagent bottles, respectively. Advantageously, said plurality of first conduits converge to said test tube via a flow diverter. Preferably, the flow divider is a multi-split pipe joint. Preferably, the flow splitter is integrally formed with the plurality of first conduits. Alternatively, the flow splitter is formed separately from the plurality of first conduits.

Preferably, one or more of the plurality of first tube-end branch conduits and the plurality of vent branch conduits is provided with an on-off valve. Alternatively, the plurality of first-tube-end branch conduits are connected to the common first conduit via a multi-way valve. Advantageously, an air pump is provided on one or more of said plurality of airway branch conduits.

Preferably, one or more of the plurality of first tube-end branch conduits, the plurality of airway conduits, is provided with an on-off valve.

Preferably, an on-off valve is disposed on one or more of the plurality of first reagent-end branch conduits.

Preferably, the pathological tissue preparation apparatus further includes a first return conduit having one end connected to the test tube and the other end connected to the first reagent bottle. The first return conduit is configured to return reagent in the test tube to the first reagent bottle, thereby enabling recycling of the reagent.

Preferably, the pathological tissue preparation apparatus further includes a return tube having one end connected to the test tube and the other end connected to the first reagent bottle.

In an advantageous embodiment, said pathological tissue preparation device further comprises: a second reagent bin in which a second reagent bottle is disposed; a second conduit connecting the second reagent bottle to the test tube in the tissue cartridge; a second pump disposed on the second conduit for delivering reagent in the second reagent bottle into the test tube.

Preferably, one or more tube sets are provided in the tissue cartridge, each tube set comprising one tube or a plurality of tubes connected in series, wherein the first and second conduits converge at one end of the tissue cartridge to a common conduit, the common conduit being connected to the respective tube set or tube sets via one or more branch conduits.

Preferably, one or more groups of tubes are provided in the tissue magazine, each group of tubes comprising one tube or a plurality of tubes connected in series, at least one tube of the one or more groups of tubes receiving reagent via only one inlet at the bottom of the tube and/or at least one tube of the one or more groups of tubes discharging reagent via only one outlet at the top of the tube.

Preferably, one or more tube sets each including one or a plurality of tubes connected in series are provided in the tissue magazine, and the pathological tissue preparation apparatus further includes a second reagent buffer bottle connected to the one or more tube sets through a second top conduit, wherein the second top conduit has, at an end remote from the second reagent buffer bottle, a corresponding one or more second top branch conduits respectively connected to the one or more tube sets. Advantageously, the second reagent buffer bottle is located in the tissue magazine or in the second reagent magazine. The second reagent buffer bottle is configured to draw reagent from the test tube and buffer the reagent therein, for example, by a pump, or push the reagent buffered therein into the test tube, thereby achieving recycling of the reagent. When there are first reagent bottle of keeping in and second reagent bottle of keeping in simultaneously, both are connected to the test tube through two ports to can realize the keeping in to different reagents. One of the first and second reagent buffer bottles may be located in the vent tube and occupy the same port at the top of the test tube as the vent tube. Preferably, an on-off valve is provided on the first and/or second conduit and/or the airway tube.

Preferably, a liquid level detector is provided on the airway tube and/or the first and/or second tubes.

Preferably, the pathological tissue preparation apparatus further comprises: the reagent box comprises a plurality of reagent bins, wherein one or more reagent bottles are arranged in each reagent bin; a reagent cartridge conduit connecting one or more reagent bottles in the reagent cartridge to the test tubes in the tissue cartridge, wherein the number of reagent cartridge conduits is greater than or equal to the number of reagent cartridges, such that each reagent cartridge is configured with at least one reagent cartridge conduit; a reagent cartridge conduit pump disposed on the reagent cartridge conduit for delivering reagent from one or more reagent bottles in the reagent cartridge into the test tube. Preferably, a temperature regulating device is arranged in the reagent cabin and used for maintaining the temperature in the reagent cabin. The maintenance temperatures set in the plurality of reagent containers may be the same or different.

Preferably, the pathological tissue preparation apparatus further comprises: a plurality of reagent temporary storage bottles for temporarily storing the reagent flowing out of the top of the test tube; a plurality of reagent bottle pipe of keeping in, it is used for connecting test tube and a plurality of reagent bottle of keeping in. Preferably, the buffer bottle is arranged in the tissue chamber, the working temperature of which varies with the temperature of the tissue chamber. Preferably, a portion of the buffer bottles is arranged in the tissue magazine and a portion of the buffer bottles is arranged in the reagent magazine. Preferably, the number of the reagent buffer bottles is the same as the number of the reagent bins, and the reagent buffer bottles are arranged in the corresponding reagent bins. In the experiment process, the reagent flowing into each reagent temporary storage bottle when temporarily storing the reagent is the reagent stored in the corresponding reagent bin.

Preferably, the pathological tissue preparation apparatus further includes: a plurality of return conduits, it is during to be made for the reagent in the test tube to flow into corresponding reagent bottle, return conduit one end is connected the top of test tube in the tissue storehouse, the reagent bottle in the reagent storehouse is connected to the other end, the degree of depth that return conduit stretches into the test tube can be adjusted according to the in-service use demand, and likewise, the degree of depth that return conduit stretches into the reagent bottle also can be adjusted according to the in-service use demand.

Preferably, the plurality of return conduits are each provided with an on-off valve, which is communicatively connected to the control device.

Preferably, when a plurality of return conduits are simultaneously connected to one test tube, a multi-inlet-one-outlet structure such as a multi-port valve may be used for the connection. Similarly, when a plurality of return conduits are connected to one reagent bottle at the same time, a multi-port valve or the like may be used for connection. Furthermore, when the plurality of return conduits are matched with the plurality of reagent bottles, the two ends of the plurality of return conduits can be connected by adopting a multi-inlet and one-outlet structure or a multi-inlet and multi-outlet structure such as a multi-way valve.

Preferably, the pathological tissue preparation device further comprises a housing, and the tissue chamber, the first reagent chamber and the second reagent chamber are all arranged in the housing.

Preferably, one or more tube sets are provided in the tissue cartridge, each tube set comprising one tube or a plurality of tubes connected in series, wherein the first conduit has at one end located in the tissue cartridge a respective one or more first tube-end branch conduits connected to the respective one or more tube sets, respectively, and the second conduit has at one end located in the tissue cartridge a respective one or more second tube-end branch conduits connected to the respective one or more tube sets, respectively.

Preferably, a plurality of first reagent bottles are provided in the first reagent compartment, and the first conduit has a plurality of first reagent end branch conduits connected to the plurality of first reagent bottles, respectively, at one end located in the first reagent compartment, and a plurality of second reagent bottles are provided in the second reagent compartment, and the second conduit has a plurality of second reagent end branch conduits connected to the plurality of second reagent bottles, respectively, at one end located in the second reagent compartment.

Preferably, a plurality of first reagent bottles are provided in the first reagent magazine, and the pathological tissue preparation apparatus has a plurality of first conduits respectively connected to the plurality of first reagent bottles from test tubes in the tissue magazine, and a plurality of second reagent bottles are provided in the second reagent magazine, and the pathological tissue preparation apparatus has a plurality of second conduits respectively connected to the plurality of second reagent bottles from test tubes in the tissue magazine. Advantageously, the first plurality of conduits is collected via a first splitter into one conduit connected to the test tube and/or the second plurality of conduits is collected via a second splitter into one conduit connected to the test tube. Alternatively, the plurality of first conduits and the plurality of second conduits are collected via a first splitter into one conduit connected to the test tube. Preferably, the first flow divider and/or the second flow divider are one-to-many pipe joints. Preferably, the first flow splitter is integrally formed with the plurality of first conduits and/or the second flow splitter is integrally formed with the plurality of second conduits. Alternatively, the first flow splitter is formed separately from the plurality of first conduits and/or the second flow splitter is formed separately from the plurality of second conduits.

Preferably, one or more of the plurality of first tube-end branch conduits and the plurality of second tube-end branch conduits are provided with a switch valve. Additionally or alternatively, a pump is disposed on one or more of the first plurality of tube end branch conduits, the second plurality of tube end branch conduits.

Preferably, an on-off valve is disposed on one or more of the plurality of first reagent end branch conduits and the plurality of second reagent end branch conduits.

Advantageously, the pathological tissue preparation apparatus further comprises a second return conduit connected at one end to the test tube and at the other end to the second reagent bottle. The second return conduit is configured to return the reagent in the test tube to the second reagent bottle, thereby enabling recycling of the reagent.

In a preferred embodiment, the pathological tissue preparation device comprises a plurality of reagent bins, one or more reagent bottles are stored in the reagent bins, the reagent bottles provide reagents for test tubes in the tissue bins, and the reagent bottles and the test tubes in the tissue bins can be connected through a conduit or a combination of a branch of the reagent conduit and the conduit.

Preferably, one or more of the plurality of reagent bins comprises a temperature control device, the temperature control device maintains the temperature in the reagent bin at a preset temperature, the preset temperature is adjustable, and the respective preset temperatures of the plurality of reagent bins can be the same or different. Preferably, the temperature control device may be a temperature detection device, a temperature adjustment device, wherein the temperature adjustment device may be a combination of a heating device and a passive cooling device (e.g. a fan), a combination of a heating device and an active cooling device (e.g. a semiconductor cooling plate, a cooling system including a compressor and a condenser), or a combination of a heating device, a passive cooling device and an active cooling device.

In a preferred embodiment, the pathological tissue preparation apparatus further comprises a temperature regulation device and a temperature sensor for detecting the internal temperature of the tissue compartment, wherein the temperature regulation device comprises a soaking device arranged inside the tissue compartment, and the soaking device is used for enabling the temperature of different positions inside the tissue compartment to be consistent; and/or the temperature regulating device comprises refrigeration equipment arranged inside the tissue bin, and the refrigeration equipment is used for cooling the tissue bin; and/or the temperature regulating device comprises a heating device arranged inside the tissue cabin, and the heating device is used for heating the tissue cabin. Preferably, the heating device is one or more of a heating wire and a heating bulb, and the refrigerating device is one or more of a semiconductor refrigerating sheet, a cooling fan, a heat exchanger and a liquid cooling device.

In another preferred embodiment, the pathological tissue preparation apparatus further includes a temperature regulation device including a liquid bath cabin provided in the tissue cabin, and a temperature sensor for detecting an internal temperature of the tissue cabin, wherein the pathological tissue preparation apparatus further includes a first reservoir for supplying a temperature regulation liquid having a first temperature range to the liquid bath cabin. Advantageously, the temperature regulation device comprises a soaking device arranged inside the tissue cabin, the soaking device being used for enabling the temperatures of different positions inside the tissue cabin to be consistent; and/or the temperature regulating device comprises refrigeration equipment arranged inside the tissue cabin, and the refrigeration equipment is used for cooling the tissue cabin; and/or the temperature regulating device comprises a heating device arranged inside the tissue cabin, and the heating device is used for heating the tissue cabin. Advantageously, the temperature regulation liquid used in the liquid bath compartment is returned to the first compartment directly or via a recovery compartment for receiving the used temperature regulation liquid from the liquid bath compartment and restoring the temperature of the received temperature regulation liquid to the first temperature range.

Advantageously, the pathological tissue preparation apparatus further comprises a second reservoir for supplying the liquid bath reservoir with a temperature-regulating liquid having a second temperature range, wherein the first reservoir and the second reservoir are connected to the liquid bath reservoir via one inlet of the liquid bath reservoir or are connected to the liquid bath reservoir via two inlets of the liquid bath reservoir, respectively. Preferably, the first temperature range is 62-66 ℃ and/or the second temperature range is 20-45 ℃. In particular, the second temperature range is room temperature. Advantageously, the temperature regulating liquid used in the liquid bath compartment is returned to the respective first or second reservoir.

Preferably, the pathological tissue preparation apparatus further includes a recovery bin for receiving the used temperature-regulating liquid from the liquid bath bin, restoring the temperature of the received temperature-regulating liquid to the first temperature range or the second temperature range, and returning the temperature-restored temperature-regulating liquid to the corresponding first bin or the second bin. Advantageously, on one or more of the lines between the first and the liquid bath bin, the second and the liquid bath bin, the line between the liquid bath bin and the recovery bin, the line between the recovery bin and the first bin and the line between the recovery bin and the second bin, a switching valve and/or a pump is provided.

Alternatively, the pathological tissue preparation apparatus further comprises: a first recovery tank for receiving the used temperature regulation liquid of the first temperature range from the liquid bath tank, recovering the temperature of the received temperature regulation liquid to the first temperature range, and returning the temperature-recovered temperature regulation liquid to the first storage tank; and a second recovery compartment for receiving the used temperature regulation liquid of the second temperature range from the liquid bath compartment, restoring the temperature of the received temperature regulation liquid to the second temperature range, and returning the temperature-restored temperature regulation liquid to the second storage compartment, wherein the first recovery compartment and the second recovery compartment are communicated to the liquid bath compartment via one outlet of the liquid bath compartment or are respectively communicated to the liquid bath compartment via two outlets of the liquid bath compartment. Advantageously, on one or more of the lines between the first and the liquid bath bin, the lines between the second and the liquid bath bin, the lines between the liquid bath bin and the first recovery bin, the lines between the liquid bath bin and the second recovery bin, the lines between the first and the second recovery bin, and the lines between the second and the second bin, there are provided on-off valves and/or pumps. Preferably, one or more of the first storage bin, the second storage bin, the first recovery bin and the second recovery bin is provided with a storage bin temperature control module and a temperature sensor, the storage bin temperature control module and the temperature sensor are electrically connected with the control device, wherein the storage bin temperature control device comprises a heating device and/or a cooling device. Advantageously, the heating device is one or more of a heating wire, a heating bulb, a temperature equalizing turbine and a temperature equalizing fan. Advantageously, the cooling device is one or more of a cooling fin, a cooling fan, a heat exchanger, a liquid cooling device, a temperature equalizing turbine and a temperature equalizing fan.

Advantageously, the recycling bin is connected to the first storage bin and the second storage bin by two water return pumps, wherein the water return pumps are electrically connected to the control device.

Advantageously, the first storage bin is connected with the first recovery bin through a first water return pump, the second storage bin is connected with the second recovery bin through a second water return pump, and the first water return pump and the second water return pump are electrically connected with the control device.

Advantageously, the internal space of the tissue compartment forms the liquid bath compartment and the test tube is immersed in the temperature-regulating liquid of the liquid bath compartment. Alternatively, the outer shell of the tissue cartridge has spaced apart inner and outer layers, the space defined between the inner and outer layers forming the liquid bath cartridge. Advantageously, the temperature regulating liquid is water and the liquid bath compartment is a water bath compartment.

Advantageously, the pathological tissue preparation apparatus further comprises a temperature regulation device comprising a liquid bath compartment disposed within the tissue compartment, and a temperature sensor for detecting the internal temperature of the tissue compartment, wherein the pathological tissue preparation apparatus further comprises a plurality of reservoirs for supplying temperature regulation liquid having a plurality of temperature ranges to the liquid bath compartment. Advantageously, the pathological tissue preparation apparatus further comprises a plurality of recovery bins for receiving used temperature regulation liquid from the liquid bath bin, restoring the temperature of the received temperature regulation liquid to one of the plurality of temperature ranges, and returning the temperature-restored temperature regulation liquid to a corresponding one of the plurality of bins.

Preferably, the plurality of temperature ranges includes three temperature ranges, respectively 60-66 ℃,40-50 ℃,20-30 ℃.

In another preferred embodiment, the pathological tissue preparation apparatus comprises a plurality of tissue bins, a plurality of first tubes, and a plurality of airway tubes, wherein one or more test tubes are disposed in each tissue bin, and wherein the one or more test tubes in each tissue bin are connected to the first reagent bottle through a corresponding first tube, and are in air communication with the exterior of the test tubes through a corresponding one of the airway tubes.

In a further preferred embodiment, the pathological tissue preparation apparatus comprises a plurality of tissue silos, a plurality of first conduits, a plurality of second conduits and a plurality of airway conduits, wherein each tissue silo is provided with one or more test tubes therein, and wherein the one or more test tubes in each tissue silo are connected to the first reagent bottle through a corresponding first conduit, to the second reagent bottle through a corresponding second conduit, and are in air communication with the exterior of the test tubes through a corresponding one of the airway conduits.

Preferably, the plurality of tissue bins have a corresponding plurality of liquid bath bins, and the pathological tissue preparation device further comprises a plurality of bins for supplying a temperature regulating liquid having a plurality of temperature ranges to the plurality of liquid bath bins. Preferably, the pathological tissue preparation apparatus further includes one or more recovery tanks for receiving the used temperature-regulating liquid from each of the plurality of liquid bath tanks, restoring the temperature of the received temperature-regulating liquid to one of the plurality of temperature ranges, and returning the temperature-restored temperature-regulating liquid to a corresponding one of the plurality of tanks.

Preferably, the first conduit has a tube inner diameter greater than a tube inner diameter of the second conduit. Advantageously or alternatively, the first conduit and the airway tube have the same tube internal diameter. Advantageously, the outer wall of one or more of the first conduit, the second conduit, the aeration conduit is provided with an insulating layer.

Advantageously, a temperature control device is arranged in the first reagent bin and/or the second reagent bin, and the temperature control device is electrically connected with the control device. Advantageously, the on-off valve is a solenoid valve.

Advantageously, the opening degree of the on-off valve can be adjusted. Additionally or alternatively, the flow rate of the first pump and/or the second pump may be adjustable.

Advantageously, an air pump is arranged on the ventilation catheter, and the air pump is electrically connected with the control device. The air pump has the functions of pressurization and decompression. Advantageously, the flow rate of the air pump is adjustable.

Preferably, the liquid bath cabin is provided with an air outlet which is connected with an air outlet switch valve or an air pump, and the air outlet switch valve or the air pump is electrically connected with the control device.

Preferably, all or part of the on-off valves are solenoid valves. Advantageously, the entire on-off valve or a part of the on-off valves are on-off valves with adjustable opening degree.

The application provides pathological tissue preparation equipment, a serial communication port, pathological tissue preparation equipment includes: a tissue chamber in which test tubes are disposed; a first reagent bin in which a first reagent bottle is disposed; a first conduit connecting the first reagent bottle to the test tube in the tissue cartridge; a first pump disposed on the first conduit for delivering reagent in the first reagent bottle into the test tube; a first return conduit having one end connected to the test tube and the other end connected to the first reagent bottle; and a control device electrically connected to the first pump.

Preferably, the first reagent bottle adopts a sealing structure, the first conduit and the first return conduit extend into the first reagent bottle, and the test tube and the first reagent bottle form a sealed experimental environment through the first conduit and the first return conduit.

Preferably, the pathological tissue preparation apparatus further comprises: a second reagent bin in which a second reagent bottle is disposed; a second conduit connecting the second reagent bottle to the test tube in the tissue cartridge; a second return conduit having one end connected to the test tube and the other end connected to the second reagent bottle; a second pump disposed on the second conduit for delivering reagent in the second reagent bottle into the test tube.

Preferably, the first reagent bottle adopts a sealing structure, the first conduit and the first return conduit extend into the first reagent bottle, when the first pump works, the second pump does not work, and the test tube and the first reagent bottle form a flowable sealed experimental environment through the first conduit and the first return conduit; and/or the second reagent bottle adopts a sealing structure, the second guide pipe and the second return guide pipe extend into the second reagent bottle, when the second pump works, the first pump does not work, and the test tube and the second reagent bottle form a flowable sealed experimental environment through the second guide pipe and the second return guide pipe.

Preferably, a plurality of first reagent bottles are arranged in the first reagent bin, wherein at least one first reagent bottle adopts a sealing structure; the pathological tissue preparation device is provided with a plurality of first conduits which are respectively connected to the plurality of first reagent bottles from the test tubes in the tissue bin, the plurality of first conduits are converged to the test tubes through a shunt, and the shunt can independently control the connection and disconnection of any first conduit; the test tubes are distributed to the plurality of first return conduits via return diverters that can individually control the make and break of any first return conduit.

The pathological tissue preparation device has the following beneficial effects that:

1. in the invention, the first pump is used for adjusting the entering and exiting of the reagent, and the reagent is automatically supplied to the test tube of the tissue bin, so that the experimental environment is established and the experiment is completed. The reagent amount of the required reagent in the test tube is small, the experimental environment is small, the required reagent amount is greatly reduced when the experiment is carried out in a small experimental environment, and the reagent amount can be controlled through the first pump. In one case, the first pump can control the entering and exiting of the reagent, realize the transition of the experimental environment, and can control the time of reagent infiltration.

2. The temperature in the tissue bins is monitored by temperature sensors, and the conversion of different experimental environments is realized by different reagents in one reagent bin or a plurality of reagent bins. In one case, an automatic dehydration and/or transparentization and/or waxing treatment and/or a wax block making process can be realized by the control device, so that the degree of automation of pathological tissues is improved, and the making quality is improved. Under a condition, through air pump and a plurality of pump mutually supporting, the business turn over speed of having accelerated reagent returns the original way of reagent for reagent can reuse, has practiced thrift reagent, has improved experimental efficiency.

3. The tissue bin is connected with the reagent bottles in the two reagent bins through the guide pipe and the switch valve, and the connection between the reagent bottles and the tissue bin is controlled through the switch valve, so that the liquid in the reagent bottles can be selected conveniently during the control experiment. The opening size of the switch valve can be adjusted, and the flow rate of the liquid in the conduit can also be adjusted. In addition, the pump speed of the first pump and the second pump can be adjusted, so that the flow rate of the liquid in the guide pipe can also be adjusted.

4. The air pump is arranged to control the pressure in the test tube so as to form negative pressure or high pressure in the test tube, so that the reagent in the reagent bottle can enter the test tube or return to the reagent bottle, the reagent can flow in the test tube in an up-and-down circulating manner, the sample is made in the fluid, the quality and the efficiency are improved, and the reagent can be reused.

5. Set up a plurality of pumps, first pipe (perhaps, first pipe and second pipe), the pipe of ventilating in same tissue storehouse for set up multiunit experimental environment (a plurality of test tubes/multiunit test tube cluster) in same tissue storehouse, make a large amount of the pathology experiments of the same type go on simultaneously, improved pathology experimental efficiency. A plurality of pumps also can receive the control of the control unit alone, and a plurality of pathology experiments are gone on under the same temperature, but the step of multiunit pathology experiment under same ambient temperature is different, also can experiment in same tissue storehouse, and then has improved pathology experimental efficiency, has increased the variety of pathology experiment in the same tissue storehouse.

6. The scheme of a plurality of tissue bins can make different pathological tissues respectively carry out automatic dehydration and/or transparent and/or wax-dipping treatment processes in a plurality of bins, each tissue bin uses corresponding manufacturing parameters, the environment in each tissue bin is controlled through the control device, the inflow and outflow of reagents are controlled, a plurality of pathological tissues are processed in parallel, the pathological tissues are made into individuation, the manufacturing quality is improved, and the pathological tissue processing efficiency is improved. Thus, the pathological tissue can be highly customized and the manufacturing time is flexible.

7. In the scheme of the first storage bin and the recovery bin, the liquid bath bin is adopted to shorten the temperature rise time in the tissue bin/the liquid bath bin, the heating equipment is arranged to further shorten the temperature rise time in the tissue bin, the manufacturing quality is improved, and the manufacturing safety is ensured. Set up low-temperature water to combine liquid bath storehouse and recovery storehouse, shortened the inside cooling time in tissue storehouse, improved work efficiency. And the refrigeration equipment is arranged, so that the cooling speed is accelerated when the tissue bin is cooled.

8. Set up temperature control equipment in first warehouse, second warehouse, recovery storehouse, liquid bath storehouse, first reagent storehouse, second reagent storehouse, be convenient for control experimental environment, be convenient for carry out the temperature to the experimental environment of difference and prepare.

Drawings

Fig. 1 shows a schematic view of a first embodiment of a pathological tissue preparation device according to the invention;

fig. 2 shows a schematic view of a second embodiment of the pathological tissue preparation device according to the invention and its variants;

figures 3, 4 and 5 show schematic views of further variants of a second embodiment of the pathological tissue preparation device according to the invention, in which the control means are not shown;

figure 6 shows a schematic view of a third embodiment of a pathological tissue preparation device according to the invention;

figures 7 and 8 show schematic views of different variants of a third embodiment of a pathological tissue preparation apparatus according to the invention, in which the control device is not shown;

fig. 9 shows a schematic view of a fourth embodiment of a pathological tissue preparation device according to the invention, in which the control means are not shown;

fig. 10 shows a schematic view of a fifth embodiment of a pathological tissue preparation device according to the invention, in which the control means and the reagent cartridge are not shown;

fig. 11 shows a schematic view of a sixth embodiment of a pathological tissue preparation device according to the invention, in which the control means are not shown;

fig. 12 shows a seventh embodiment of the pathological tissue preparation device according to the invention, in which only the tissue and reagent cartridges are shown;

fig. 13 shows a schematic view of an eighth embodiment of the pathological tissue preparation device according to the invention.

Detailed Description

To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It should be understood that the criss-crossing conduits, lines or circuits shown in the various figures exclude connection or communication with each other.

Fig. 1 shows a schematic view of a first embodiment of a pathological tissue preparation device according to the invention. The pathological tissue preparation device, in particular an incubator, comprises a tissue chamber 2, a first reagent chamber 3 and a control device 5. The test tube 21 is placed in the tissue chamber 2, and the test specimen 10 is placed in the test tube 21. The tube may be a disposable, replaceable consumable. The actual product shape of the test tube may be similar to a drip cup used in an intravenous set. The first reagent chamber 3 is disposed with a first reagent bottle 31, and the first reagent bottle 31 is connected with the test tube 21 in the tissue chamber 2 through a first conduit 61. A first pump 81 is provided in the first conduit 61, and the liquid (reagent) in the first reagent bottle 31 is transferred to the test tube 21 by the first pump 81. The first pump 81 may return the reagent in the test tube 21 to the first reagent bottle 31. The first pump 81 may be a liquid pump. The test tube 21 is also connected to the outside environment by an air duct 71, on which air pump 85 can be arranged on the air duct 71. The first pump 81 and the air pump 85 are each electrically connected (i.e., communicatively connected) to the control device 5 and are operated under control, thereby controlling the reagent in the test tube 21 and completing the experiment.

Fig. 2 shows a schematic view of a second embodiment of the pathological tissue preparation device according to the invention and its variants. On the basis of the above embodiment, a second reagent magazine 4 in which another reagent bottle (i.e., the second reagent bottle 41) is placed is further provided in the pathological tissue preparation apparatus. The second reagent bottle 41 is connected to the test tube 21 in the tissue chamber 2 via a second conduit 62. A second pump 82 is provided on the second conduit 62 for delivering reagent into or out of the test tubes 21 in the tissue cartridge 2. The same reagent can be placed in the first reagent bottle 31 and the second reagent bottle 41. The two reagent bottles (i.e. the first reagent bottle 31 and the second reagent bottle 41) can also contain different reagents to meet different experimental environment requirements, so that the reagents in the reagent bins do not need to be adjusted in the experimental process.

In a variation of the second embodiment, the first conduit 61, the second conduit 62 and/or the airway conduit 71 are provided with a first switch valve 91, a second switch valve 92, a fifth switch valve 95 for controlling the liquid transport within each conduit and thus the entry of different reagents into the tissue chamber 2 at different stages of the experiment. The first pump 81, the second pump 82, and the air pump 85 control inflow and outflow of the respective reagents. A level detector (not shown) is provided on the vent conduit 71 and/or a level detector (not shown) is provided on the first and

second conduits

61, 62, which are communicatively connected to the control device 5 to detect the level of liquid in the test tube 21.

When the reagent is supplied to the cuvette 21, the air pump 85 is in an exhaust state or an idle state, and the first pump 81 or the second pump 82 is adjusted to be in a liquid supply state. When the liquid level detector (liquid sensor) detects liquid, the air pump 85 and the first pump 81 or the second pump 82 are stopped. When it is necessary to replace the reagent, the air pump 85 is turned on to supply air, the first pump 81 or the second pump 82 is rotated in the reverse direction or in an idling state, and the air pump 85 supplies air to the test tube 21 through the air vent tube 71 to discharge the liquid out of the test tube 21 (at this time, the test tube 21 is sealed by a cap, not shown). When the liquid level detector of the first conduit 61 or the second conduit 62 detects that the liquid level has fallen below the predetermined position, the air pump 85 and the first pump 81 or the second pump 82 are stopped. Then, the steps at the time of reagent input are repeated, and a replacement reagent is input into the test tube 21.

Fig. 3 shows a schematic view of another variant of the second embodiment of the pathological tissue preparation device according to the invention. On the basis of the above embodiment, the test tube ends of the first conduit 61 and the second conduit 62 may be collected into a common conduit to be connected to the test tube 21. At this time, the reagents in the first and

second reagent bottles

31 and 41 can be selected to enter the test tube 21 by the first and

second switching valves

91 and 92 and/or the first and

second pumps

81 and 82.

In a further variant of the second embodiment shown in fig. 4, the pathological tissue preparation apparatus also has a first return conduit 63 and a second return conduit 64 connected between the test tube 21 and the first and

second reagent bottles

31 and 41, respectively. The first return conduit 63 and the second return conduit 64 are used for returning the reagent in the test tube 21 to the corresponding first reagent bottle 31 and the second reagent bottle 41, so that the recycling of the reagent is realized. The first return conduit 63 and the second return conduit 64 may have on-off valves and/or pumps. Similarly, in the first embodiment shown in fig. 1, a first return conduit 63 may also be provided between the test tube 21 and the first reagent bottle 31.

Meanwhile, the length of the first return conduit 63 extending into the test tube 21 can be adjusted according to actual use requirements; the length of the first return conduit 63 extending into the corresponding first reagent bottle 31 and second reagent bottle 41 can also be adjusted according to the actual use requirement. For example, when the reagent flows through the test tube 21 and requires two-way circulation, the first return conduit 63 extends into the first reagent bottle 31 and the second reagent bottle 41 for a long length, the first return conduit 63 extends into the bottoms of the first reagent bottle 31 and the second reagent bottle 41 almost, and in combination with the use of the first pump 81, the reagent can enter the test tube 21 from the first conduit 61 and also enter the test tube 21 from the first return conduit 63. When the reagent flows through the test tube 21 and requires one-way circulation, the first return conduit 63 extends into the first reagent bottle 31 and the second reagent bottle 41 for a short length, and the reagent liquid level in the first reagent bottle 31 and the second reagent bottle 41 does not exceed the bottom of the first return conduit 63. At this time, when the first pump 81 is operated in the forward direction, the reagent is continuously introduced into the test tube 21 from the first reagent bottle 31 and the second reagent bottle 41 through the first conduit 61, and is returned to the corresponding first reagent bottle 31 and second reagent bottle 41 from the first return conduit 63, so as to realize the recycling of the reagent. When the first pump 81 is operated in reverse, the first return conduit 63 sucks air from the first and

second reagent bottles

31 and 41, and the reagent is returned to the respective first and

second reagent bottles

31 and 41 through the first conduit 61.

In a further variant of the second embodiment shown in fig. 5, a first reagent buffer bottle 65 and a second reagent buffer bottle 66 are also provided in the pathological tissue preparation device. The first reagent buffer bottle 65 and the second reagent buffer bottle 66 are connected to the test tube 21 via a first top conduit and a second top conduit, respectively, for buffering the reagent flowing out of the test tube 21 therein, or pushing the reagent buffered therein into the test tube 21, thereby realizing the recycling of the reagent. For example, a first reagent buffer bottle 65 is used to buffer the reagent originally from the first reagent bottle 31, and a second reagent buffer bottle 66 is used to buffer the reagent originally from the second reagent bottle 41. One of the first reagent buffer bottle 65 and the second reagent buffer bottle 66 can be located on the vent line 71. Similarly, in the first embodiment shown in fig. 1, a first reagent buffer bottle 65 can be provided which is connected to the test tube 21 via a first top line. The reagent buffer bottles are located in the tissue magazine 2 (as shown in fig. 5) and can also be located in the corresponding reagent magazine. A fill level detector (not shown) is arranged in the first reagent buffer bottle, the second reagent buffer bottle or on the line for venting corresponding to the first reagent buffer bottle, the second reagent buffer bottle, which is communicatively connected to the control device 5 in order to detect the fill level of the first reagent buffer bottle, the second reagent buffer bottle or the line for venting.

Fig. 6 shows a schematic view of a third embodiment of the pathological tissue preparation device according to the invention. On the basis of the above-described embodiment, a plurality of reagent bottles (two first reagent bottles 31 or two second reagent bottles 41 are shown in fig. 6) are contained in the first reagent magazine 3 and/or the second reagent magazine 4. It should be noted that the arrangement of the reagent bottles shown in the figures is only schematic, and a plurality of reagent bottles may be arranged in other ways. For example, a plurality of reagent bottles may be arranged in two or more layers, each of which may house one or more reagent bottles. A plurality of reagent bottles containing different reagents or the same reagent therein, each reagent bottle being independently connected to the first conduit 61 or the second conduit 62 through a corresponding plurality of reagent end branch conduits to the first switching valve 91, the second switching valve 92, the third switching valve 93, the fourth switching valve 94. Each reagent bottle corresponds to the independent first switch valve 91, second switch valve 92, third switch valve 93, fourth switch valve 94, and the first switch valve 91, second switch valve 92, third switch valve 93, fourth switch valve 94 are electrically connected (i.e., communicably connected) with the control device. The combination of the two pumps (i.e., the first pump 81 and the second pump 82) can realize that a plurality of reagent bottles in a plurality of reagent bins (i.e., the first reagent bin 3 and the second reagent bin 4) are respectively connected with the test tubes 21 in the tissue bin 2, and through the control device 5, the reagent in any reagent bottle can enter the test tube 21 from the reagent bottle at any time or can be discharged into the reagent bottle from the test tube 21 at any time. As shown in fig. 6, for example, two first reagent bottles 31 in the first reagent chamber 3 share the first on-off valve 91 and a section of the first conduit 61 downstream of the third on-off valve 93, so that in a preferred embodiment, two reagent bottles in the same reagent chamber are used to contain the same reagent in order to avoid mutual contamination of the reagents. In the embodiment shown in fig. 6, two pumps (i.e., a first pump 81, a second pump 82) are located on a common section of the first conduit 61, the second conduit 62, respectively. In a variant, instead of providing a pump on the above-mentioned common section, a pump and/or a switch valve may be provided on each reagent-end branch conduit.

The two on-off valves (e.g., first on-off valve 91, third on-off valve 93) on the same conduit may be replaced by a controlled three-way valve operable to selectively communicate the first conduit 61 with either the first reagent bottle 31 or the second reagent bottle 41 to perform the function of the two on-off valves in the figure. Similarly, when there are multiple on-off valves, they can be replaced by one controlled multi-way valve. The multi-way valve is located at the junction of the plurality of reagent end branch conduits with the common first conduit 61 so that the first conduit 61 can be selectively communicated with the respective reagent bottle by controlling the multi-way valve. Alternatively, the three-way valve or multi-way valve is used for gating the flow path of the reagent, and its material or coating can withstand corrosion by chemical agents including but not limited to various concentrations of alcohol, benzene solution, paraffin (high temperature molten state); optionally, the three-way valve or multi-way valve can withstand repeated changes in temperature, with the temperature range at least satisfying 0 ℃ to 70 ℃.

The pathological tissue preparation device is further provided with a shell (not shown), wherein a tissue bin 2, a first reagent bin 3 and a second reagent bin 4 are arranged in the shell, and the shell is used for protecting structures in the shell.

Fig. 7 shows a schematic view of a variant of the third embodiment of the pathological tissue preparation device according to the invention. On the basis of the above described embodiment, the different reagent bottles are connected to the test tube via completely separate conduits, i.e. without a common conduit section, with separate liquid pumps on the completely separate conduits for separately feeding reagents into the test tube.

In another variation of the third embodiment shown in fig. 8, the first plurality of conduits connected to the first plurality of reagent bottles 31 in the first reagent magazine 3 may be pooled into one conduit, which is then connected to the test tube 21, via the first flow splitter 67, and the second plurality of conduits connected to the second plurality of reagent bottles 41 in the second reagent magazine 4 may be pooled into one conduit, which is then connected to the test tube 21, via the second flow splitter 68. In a further variant, the first and second plurality of conduits may be collected into one conduit via the same flow splitter and subsequently connected to the test tube 21. The flow divider, the first flow divider and the second flow divider are all used for liquid flow division and can be a liquid flow division structure combined with an electric control switch valve. The function of these diverters is to control the opening and closing of the various passages of the diverter by means of electrically controlled multi-switch valves for selectively controlling which of the first plurality of conduits can be circulated. These diverters may also be replaced with liquid tees or liquid multipaths. Optionally, the diverter, first diverter, second diverter are used to gate the flow paths of the reagents, the materials or coatings of which are able to withstand corrosion from chemical reagents including but not limited to alcohols of various concentrations, benzene solutions, paraffin (high temperature molten state); optionally, the flow divider, the first flow divider, and the second flow divider can withstand repeated changes in temperature, wherein the temperature range is at least 0 ℃ to 70 ℃.

Fig. 9 shows a schematic view of a fourth embodiment of a pathological tissue preparation device according to the invention, in which the control means are not shown. On the basis of the above-described embodiment, a plurality of test tubes (three test tubes 21 are shown in fig. 9) are contained in one tissue magazine 2. In this embodiment, with multiple tubes, multiple experimental environments can be run in parallel, and multiple experiments can be run either synchronously or asynchronously. Specifically, there are a plurality of cuvette-end branch conduits that are merged into the common first conduit 61, a plurality of cuvette-end branch conduits that are merged into the common second conduit 62, and a plurality of ventilation conduits 71, in accordance with the number of cuvettes. The first reagent chamber 3 is connected to the plurality of test tubes in the tissue chamber 2 via a first pump 81, a first conduit 61, and a plurality of test tube end branch conduits, respectively, and the second reagent chamber 4 is connected to the plurality of test tubes in the tissue chamber 2 via a second pump 82, a second conduit 62, and a plurality of test tube end branch conduits, respectively. A plurality of ventilation tubes 71 are connected to the air pump. Some or all of the plurality of tube-end branch conduits and/or the plurality of air-vent conduits 71 are provided with on-off valves. The pumps, conduits, on-off valve structures provided in the same tissue chamber 2 may be controlled individually or together. If the experimental steps are the same, multiple sets of experiments can be controlled in the same manner. If the experimental steps are different, multiple sets of experiments are controlled in different ways to perform different experiments in the environment that the same tissue magazine 2 can provide. By using the same tissue bin 2 and the operation steps of temperature adjustment of the same group, a plurality of groups of experiments can be performed in the same tissue bin in parallel, so that the efficiency of pathological experiments is improved, and a new tissue bin and corresponding temperature increasing and decreasing steps required by the experiments are eliminated.

Fig. 10 shows a schematic view of a fifth embodiment of a pathological tissue preparation apparatus according to the invention, in which the control device and the reagent cartridge are not shown. On the basis of the above embodiment, a plurality of tissue bins (three tissue bins 2 are shown in fig. 10) are provided, and each tissue bin may be a test tube experimental environment or a plurality of experimental environments. It should be noted that the layout of the test tubes shown in fig. 10 is only illustrative, and the plurality of test tubes may be arranged in other ways. For example, the plurality of cuvettes may be arranged one above the other in two or more layers, each of which may house one or more cuvettes. In particular, there are tissue cartridges 2, each connected to a reagent cartridge or to the external environment through a respective duct, airway tube, on-off valve, pump, etc., which are not described in detail herein. A temperature sensor (not shown) is disposed in each tissue cartridge, and the individual tissue cartridges may have the same or different temperatures. Each tissue chamber has a separate conduit, airway tube, first pump and second pump so that the automatic dehydration, transparentization and/or waxing processes can be performed independently and without interference. Therefore, the parallel and non-simultaneous experiment of a plurality of organization bins can be realized, the experiment flexibility is increased, and the experiment efficiency is improved.

Advantageously, in various embodiments, the first pump and/or the second pump of each tissue compartment is adjustable in flow rate and adjustable in flow direction. The flow rate of the liquid in the conduit is controlled by controlling the flow rate of the first pump and/or the second pump, thereby controlling the experimental environmental parameters.

Advantageously, in various embodiments, a temperature control structure and a temperature detection structure are further provided. Specifically, a temperature sensor is arranged in the tissue cabin and electrically connected with the control device, so that the control device monitors the temperature in the control cabin.

Advantageously, the tissue chamber is provided with a refrigeration device, which cools the tissue chamber. Additionally or alternatively, the tissue cartridge is provided with a heating device that warms the tissue cartridge. The heating device can be one or more of a heating wire and a heating bulb. The refrigerating equipment can be one or more of a semiconductor refrigerating sheet, a cooling fan, a heat exchanger and liquid cooling equipment. In addition, the tissue cabin can be also provided with a temperature equalizing device which can be a temperature equalizing fan (for equalizing the temperature of air) and a temperature equalizing turbine (for equalizing the temperature of temperature regulating liquid in the liquid bath cabin). The temperature environment in the tissue cabin can be changed through the refrigerating equipment and/or the heating equipment and/or the temperature equalizing equipment, and the requirements of different experimental contents and different experimental steps on the environment temperature are met.

Fig. 11 shows a schematic view of a sixth embodiment of the pathological tissue preparation device according to the invention, in which the control means are not shown. As shown in fig. 11, the pathological tissue preparation apparatus further includes a first storage chamber (in particular, a high-temperature water chamber 6), a second storage chamber (in particular, a low-temperature water chamber 7), and a first recovery chamber 8 and a second recovery chamber 9. The tissue magazine 2 shown in fig. 11 is in its entirety in the form of a liquid bath magazine (in particular, a water bath magazine), i.e. the interior space of the tissue magazine forms a liquid bath (in particular, a water bath) in which the test tube 21 is partially or completely immersed. At this time, the test tube 21 is sealed so that the liquid in the liquid bath does not enter the test tube 21. Alternatively, the liquid bath compartment may be formed in an interlayer of the outer shell of the tissue compartment 2, i.e. the outer shell of the tissue compartment 2 may be formed as spaced apart inner and outer layers and a liquid bath formed between the inner and outer layers, in which the test tube 21 is not immersed. The liquid bath cabin is connected with a high-temperature water cabin 6 and a low-temperature water cabin 7 through a switch valve and a conduit. The connection interface for connecting the high-temperature water bin 6 and the low-temperature water bin 7 is positioned at the bottom of the liquid bath bin. When a high-temperature environment is needed in the liquid bath bin, the switch valve connected with the high-temperature water bin 6 is opened, high-temperature liquid in the high-temperature water bin 6 enters the liquid bath bin through the guide pipe, and when the high-temperature liquid reaches a preset height, the switch valve connected with the high-temperature water bin 6 is closed. At this point, the temperature control device in the liquid bath compartment adjusts/maintains the temperature in the liquid bath compartment. When a low-temperature environment is needed in the liquid bath cabin, the switch valve connected with the first recovery cabin 8 is opened, and high-temperature water flows into the first recovery cabin 8 through the guide pipe. The first recovery bin 8 maintains the temperature of the high-temperature liquid through the temperature control device, and the high-temperature liquid can be recovered to the high-temperature water bin 6 through the first water return pump 87, so that the liquid can be recycled. When the high-temperature liquid in the liquid bath bin is exhausted, the switch valve connected with the first recovery bin 8 is closed, and the switch valve connected with the low-temperature water bin 7 is opened, so that the low-temperature liquid flows into the liquid bath bin through the guide pipe, and the rapid cooling of the experimental environment in the liquid bath bin is realized. The temperature in the liquid bath cabin can be maintained by the temperature control device in the liquid bath cabin. When the low-temperature liquid in the liquid bath cabin needs to be emptied, the switch valve connecting the liquid bath cabin and the second recovery cabin 9 is opened, and the low-temperature liquid in the liquid bath cabin is discharged. The temperature control device in the second recovery bin 9 controls/maintains the temperature of the low-temperature liquid, and when the temperature of the low-temperature liquid meets the temperature requirement of the low-temperature liquid, the low-temperature liquid in the second recovery bin 9 is sent to the low-temperature water bin 7 through the second water return pump 88. In a simplified variant, the pathological anatomy creating device can have only one recovery bin. In another more simplified variant, the pathological tissue preparation device may not have a recovery tank, and the water in the bath tank may be returned directly to the corresponding high-temperature or low-temperature water tank, where it is returned to the high or low temperature by the temperature control means.

In one variant, the pathological tissue preparation device comprises only the high-temperature water sump 6 and not the low-temperature water sump 7. When the temperature requirement of the experimental environment on the low-temperature environment is not strict, the pathological tissue manufacturing equipment only comprises a high-temperature water bin 6, when the low-temperature environment is needed in the liquid bath bin, no water is added into the liquid bath bin, or the water stored in the liquid bath bin is discharged into a recovery bin, and then the environmental cooling is realized through natural cooling or other liquid bath-free modes such as air cooling and the like; optionally, a reagent (cryogenic liquid) is introduced into the test tube 21, and the temperature requirement in the test tube 21 is met by introducing the cryogenic liquid into the test tube for performing the experiment. When a high-temperature environment is needed in the liquid bath cabin, the switch valve connected with the high-temperature water cabin 6 is opened, high-temperature water in the high-temperature water cabin 6 enters the liquid bath cabin through the guide pipe, and when the high-temperature water reaches a preset height, the switch valve connected with the high-temperature water cabin 6 is closed. And when the experiment is finished, opening the switch valve of the water outlet of the liquid bath bin, and emptying the high-temperature water in the liquid bath bin. The temperature control device of the recovery bin adjusts the water in the recovery bin to the temperature of the high-temperature water bin 6, and when the water temperature in the first recovery bin 8 reaches the temperature of the high-temperature water bin 6, the first water return pump 87 is started to return the water in the first recovery bin 8 to the high-temperature water bin 6, so that the high-temperature water can be recycled; or the high-temperature water in the liquid bath bin is directly discharged back to the high-temperature water bin, and the temperature is recovered in the high-temperature water bin.

Preferably, the first storage bin and the liquid bath bin realize liquid circulation through a pump control, and liquid flowing type temperature control can also be realized, namely, the liquid in the liquid bath bin continuously flows. During the experiment, when the first temperature is required to be maintained, the liquid at the first temperature in the first storage bin is continuously supplied into the liquid bath bin, the liquid at the first temperature in the liquid bath bin flows out of the liquid bath bin, and the liquid bath bin is maintained at the first temperature, so that the influence of heat transfer is minimized. Meanwhile, when the liquid bath cabin needs to be maintained at the second temperature, the liquid at the second temperature in the second storage cabin is continuously supplied into the liquid bath cabin, the liquid at the second temperature in the liquid bath cabin flows out of the liquid bath cabin, and the liquid bath cabin is maintained at the second temperature, so that the influence of heat transfer is minimized.

Preferably, the first storage bin and the recovery bin (or the first recovery bin) and the liquid bath bin realize liquid circulation through a pump; the second storage bin and the recovery bin (or the second recovery bin) and the liquid bath bin realize liquid circulation through a pump; the third storage bin and the recovery bin (or the third recovery bin) realize liquid circulation with the liquid bath bin through a pump, and so on.

In another variant, more structural and positional relationships may be defined in order to achieve the regulation of the height of the water in the control liquid bath compartment. For example, one or more liquid level detectors are disposed in the liquid bath compartment, the liquid level detectors are installed at different heights in the liquid bath compartment, and the height of the liquid level in the liquid bath compartment is determined by the liquid level detectors.

The high temperature water bin 6 and/or the low temperature water bin 7 are/is arranged at a position higher than the tissue bin 2, and liquid in the high temperature water bin 6 and/or the low temperature water bin 7 flows into the tissue bin 2/the liquid bath bin by utilizing gravity. The recovery bin is lower than the tissue bin, and liquid in the liquid bath bin flows into the recovery bin by using gravity. Or the first recovery bin 8 and the second recovery bin 9 are both lower than the tissue bin 2, and the liquid in the liquid bath bin flows into the first recovery bin 8 and the second recovery bin 9 by utilizing gravity.

Alternatively, the high temperature water sump 6 and/or the low temperature water sump 7 is connected to the liquid bath sump by a water pump (not shown), and the recovery sump is connected to the liquid bath sump by a water pump (not shown). Alternatively, the first recovery bin 8 and the second recovery bin 9 are connected with the liquid bath bin through water pumps (not shown). The water pump controls the liquid in the liquid bath cabin to flow in and out.

On the basis of the above modification, the temperature regulating liquid in the liquid bath cabin may be water, or may be other temperature regulating liquid. The temperature regulating liquid should have a high specific heat capacity in order to achieve the effect of quickly regulating the temperature in the liquid bath tank.

On the basis of the above embodiment or its modification, the tissue cabin further comprises an air pump, the air pump is connected with the tissue cabin 2 through an air pipe and a switch valve, the air pump has both the pressurization and decompression functions, and the switch valve is electrically connected with the control device 5. The air pump and the air pipe can be matched with a switch valve/a water pump of a high-temperature water bin, a low-temperature water bin and a recovery bin (or a first recovery bin and a second recovery bin) to finish water injection and drainage in the liquid bath bin.

On the basis of the above-described embodiment or its modification, the pathological tissue preparation apparatus includes a plurality of bins for supplying a temperature-regulating liquid having a plurality of temperature ranges to the liquid bath bin, and/or includes a plurality of recovery bins. The recovery bins are used for receiving the used temperature regulating liquid from the liquid bath bin, restoring the temperature of the received temperature regulating liquid to a corresponding one of the temperature ranges, and returning the temperature-restored temperature regulating liquid to a corresponding one of the bins.

In various embodiments, the tissue silo 2 also contains a soaking fan and/or turbine (not shown) inside. The soaking fan is used to rapidly equilibrate the temperature inside the tissue chamber 2 as the tissue chamber 2 adjusts temperature in the absence of liquid. The turbine is used to rapidly equilibrate the temperature inside the tissue chamber 2 when there is liquid in the tissue chamber 2. The tissue bin 2 is provided with a refrigerating device, and the refrigerating device is used for accelerating the cooling speed when the tissue bin 2 needs to be cooled. Organize 2 inside heating equipment that are provided with in storehouse, heating equipment is used for accelerating the programming rate when organizing 2 needs intensification in storehouse to improve experimental efficiency.

In various embodiments, the reagent in different reagent bins has different viscosity and different temperature-dependent characteristics. The experiment requires that reagents with different temperatures and viscosities are flowed in different experimental stages, so that more structures are defined. For example, the first conduit 61 may have a larger inner diameter than the second conduit 62, and the second conduit 62 may have a larger inner diameter than the first conduit 61. The outer walls of the first conduit, the aeration conduit and the second conduit can be provided with heat preservation layers. The conduit with the large inner diameter of the pipe is used for flowing liquid with poor fluidity, so that the conduit is prevented from being blocked. The heat preservation is used for avoiding the liquid temperature rapid change in the pipe to avoid liquid mobility in the pipe to reduce, and then avoid liquid temperature change to influence the experimental result.

On the basis of the above embodiment, the first reagent chamber 3 may contain a plurality of reagent bottles therein, and each reagent bottle is connected to the first conduit 61 through an on-off valve and a conduit. The second reagent chamber 4 contains a plurality of reagent bottles, each connected to the second conduit 62 through a switching valve and a conduit. The first reagent compartment 3 and/or the second reagent compartment 4 may contain a temperature control device therein. The switch valve and the temperature control device are electrically connected with the control device. The ambient temperature in the two reagent compartments may be different. For example, the first reagent chamber 3 can be adjusted to an ambient temperature of 66 ℃, and the first reagent chamber can be used for holding a substance which is solidified at a normal temperature and melted at a high temperature, such as paraffin. The second reagent chamber 4 can be maintained at room temperature, and the second reagent chamber can be used for holding liquid substances at room temperature, such as alcohol and benzene solutions with various concentrations. A plurality of reagent bottles can be placed in the reagent bin, so that a plurality of solutions with the same environmental property can be placed in the same reagent bin at the same time. Therefore, the control device controls the switch valve to select the solution in the experiment, and the automation degree and the experiment efficiency are further improved.

In various embodiments, the air pump is arranged on the air duct, and the connection mode of the air pump and the test tubes is various. For example, the air pump is provided with one, a plurality of air ducts are converged together to be communicated with the air pump, and each air duct is provided with a switch valve. The switch valve is electrically connected with the control device and is controlled by the control device.

Alternatively, two air pumps are provided, a plurality of first air ducts are gathered to be communicated with the first air pump, a plurality of second air ducts are gathered to be communicated with the second air pump, and each first air duct and each second air duct are provided with a switch valve. The switch valve is electrically connected with the control device and is controlled by the control device.

In each embodiment, the test tubes 21 in the tissue chamber 2 are provided with test tube openings at the upper and lower ends thereof, and the upper and lower test tube openings are respectively connected with the ventilation catheter and the first catheter (or the first catheter and the second catheter) through catheters. Such connection forms a circuit for the circulation of the liquid. The tissue sample is placed in a test tube. The experimental process is automatically controlled by controlling the opening and closing states of the switch valves, the pumps and the like through the control device, so that a more complete automatic experimental process is realized.

In various embodiments, the plurality of switching valves described above employ solenoid valves. The switch valves in the invention can select different types of electromagnetic valves according to the purposes of the switch valves, for example, the switch valve in the liquid conduit selects the electromagnetic valve for controlling liquid; and the switch valve on the air pipe selects the electromagnetic valve for controlling the air.

Preferably, the opening size (opening degree) of each of the switching valves is controllable, that is, the opening sizes of the gas switching valve and the liquid switching valve are adjusted in a multi-stage or stepless manner, so that the flow rate of the gas or the liquid can be controlled by the switching valves. The first pump (liquid pump), the second pump (liquid pump) and the air pump are matched with the switch valves on the corresponding conduits/pipelines of the pumps, so that the flow rate of liquid/gas can be adjusted, and the pressure of the liquid/gas can be adjusted. It is envisaged that the pumping speed of each pump may also be adjustable, so that the liquid/gas flow rate may be adjusted by controlling the pumping speed. Additionally or alternatively, the liquid/gas flow rate is regulated by the coordinated control of the on-off valve and the pump.

Fig. 12 shows a seventh embodiment of the pathological tissue preparation apparatus according to the invention, in which only the tissue and reagent cartridges are shown. On the basis of the above embodiment, in order to increase the number of experimental environments, some or all of the plurality of test tubes in the tissue chamber may be connected in series by a conduit. In this way, one experimental environment can be changed to a plurality of experimental environments in series, reducing the number of pumps and conduits between the reagent and tissue compartments.

The first and second conduits may have a variety of connections to a plurality of reagent bottles in the reagent cartridge. For example, multiple reagent bottles may be connected to the tissue cartridge using one reagent input pathway. In this embodiment, a plurality of tissue magazines 2 are provided, each of which has a plurality of sets of test tubes in series. The tube-end branch conduits corresponding to the bottommost tubes (e.g., the lower three tubes 21 in fig. 12) of the sets of series-connected tubes in each tissue magazine converge into a common first conduit 61. The first pipe 61 is connected to a plurality of first reagent bottles 31 in the first reagent magazine 3 via a first pump 81, the plurality of first reagent bottles 31 are connected to the first pipe 61 via a plurality of reagent end branch pipes, and a control switch valve is provided on each of the reagent end branch pipes. The tube-end branch conduits corresponding to the bottommost tube (e.g., the lower three tubes 21 in fig. 12) of the sets of series-connected tubes in each tissue magazine are pooled to a common second conduit 62. The second pipe 62 is connected to a plurality of second reagent bottles 41 in the second reagent magazine 4 via a second pump 82, the plurality of second reagent bottles 41 are connected to the second pipe 62 via a plurality of reagent end branch pipes, respectively, and a control switch valve is provided on each of the reagent end branch pipes. The connection of the plurality of reagent-end branch conduits to the first and

second conduits

61, 62 and/or the connection of the plurality of test tube-end branch conduits to the first and

second conduits

61, 62 may be by a multilumen tube or shunt. The airway branch conduits corresponding to the topmost test tube (e.g., the top three test tubes 21 in fig. 12) in the sets of series connected test tubes in each tissue chamber converge into a common airway conduit 71, and the airway conduit 71 is connected to an air pump 85. In this example, the number of reagent bottles is independent of the number of pumps, there are only two pumps, and the number and type of reagent bottles are adjusted according to the experimental requirements.

In one variation, there is a set of reagent input pathways for each reagent bottle. In this variant, a plurality of tissue magazines 2 are provided, each of which has a plurality of sets of test tubes in series. Corresponding to the plurality of first reagent bottles 31 in the first reagent magazine 3, there are a corresponding number (in particular, the same) of the plurality of first conduits 61 and the plurality of first pumps 81. Similarly, there are a corresponding number (in particular, the same) of the plurality of second conduits 62 and the plurality of second pumps 82, corresponding to the plurality of second reagent bottles 41 in the second reagent tank 4. The bottommost tube of the sets of series-connected tubes in each tissue magazine contains a corresponding (in particular, identical) number of multiple tube-end branch tubes, which converge to a corresponding first tube and a corresponding second tube, respectively. For example, if m reagent bottles are contained in the first reagent compartment 3 and n reagent bottles are contained in the second reagent compartment 4, m first conduits 61 (and m first pumps 81) and n second conduits 62 (and n second pumps 82) are provided correspondingly. This ensures that a single vial is connected to multiple sets of test tubes in series using a single conduit. The number of conduits and pumps is related to, in particular the same as, the number of reagent bottles. The type and the quantity of the reagent bottles are adjusted according to the experiment requirements.

In another variant, the case of a plurality of reagent bottles corresponding to one reagent input path occurs simultaneously with the case of a single reagent bottle corresponding to one reagent input path, i.e. the number of sets of test tubes in series does not correspond to the number of pumps. The quantity of reagent bottle is irrelevant with the quantity of pump, and the quantity of pump is relevant with the quantity in reagent storehouse, and under the condition in two reagent storehouses, the pump only has two, gets into the business turn over through pump and ooff valve's cooperation regulation reagent, and the reagent bottle is according to experiment demand adjustment kind and quantity. It is envisaged that where a plurality of tissue compartments are provided, the number of pumps will be 2 times the number of tissue compartments (in the case of two reagent compartments).

In various embodiments, the conduits interconnected in the present invention may be provided as integrally formed branch conduits. Alternatively, the conduits are formed separately and joined as a sealed conduit during assembly. Further, in the conduits used in the above embodiments, all or part of the conduits are heat-insulating conduits, or a heat-insulating layer is disposed outside the conduits, and is used for heat-insulating high-temperature liquid during transmission and/or heat-insulating low-temperature liquid during transmission.

In various embodiments, the first conduit (or first and second conduits) in the tissue cartridge and the airway tube may be connected in a variety of ways and arrangements. Specifically, a plurality of first conduits (or first and second conduits) in the tissue chamber are distributed at the bottom of the tissue chamber and a plurality of exhaust conduits are distributed on the sidewall of the tissue chamber. The plurality of first conduits (or the first and second conduits) and the plurality of airway tubes are arranged in a regular pattern, such as equidistant, around the side wall of the tissue chamber, etc.

Further, a plurality of first conduits (or first and second conduits) may pass through the tissue deck. Alternatively, the top end of the first conduit (or the first and second conduits) is flush with the bottom end of the tissue chamber, or the bottom end of the first conduit (or the first and second conduits) is flush with the bottom end of the tissue chamber. The reagent end of the first conduit (or the first and second conduits) may be provided with a plurality of conduit diverters, with a plurality of interface sides of the conduit diverters connected to each reagent cartridge by a conduit. Similarly, the cuvette end of the first conduit (or the first and second conduits) may be provided with a plurality of conduit diverters, with a plurality of interface sides of the conduit diverters being connected to each cuvette by the conduit. The outer wall of part of or all the guide pipes is wrapped with an insulating layer. A plurality of airway tubes may be passed through the tissue cartridge sidewall. Alternatively, the top end of the airway tube is flush with the tissue chamber sidewall, or the bottom end of the airway tube is flush with the tissue chamber sidewall. The top ends of the plurality of the aeration pipes are connected with an air pump. The air pump is a bidirectional air pump, and can output air or input air. The air pump is connected with a conduit which is communicated with the external environment.

Fig. 13 shows a schematic view of an eighth embodiment of the pathological tissue preparation device according to the invention. The pathological tissue preparation device, in particular an incubator, comprises a tissue chamber 2, a first reagent chamber 3 and a control device 5. The test tube 21 is placed in the tissue chamber 2, and the test specimen 10 is placed in the test tube 21. The tube may be a disposable, replaceable consumable. The actual product shape of the test tube may be similar to a drip cup used in an intravenous set. The first reagent chamber 3 is disposed with a first reagent bottle 31, and the first reagent bottle 31 is connected with the test tube 21 in the tissue chamber 2 through a first conduit 61. The first conduit 61 is provided with a first pump 81, and the liquid (reagent) in the first reagent bottle 31 is transferred to the test tube 21 by the first pump 81. The first pump 81 may also return the reagent in the test tube 21 to the first reagent bottle 31. The first pump 81 may be a liquid pump. The test tube 21 is also connected to the first reagent bottle 31 through a first return conduit 63. The first pump 81 is electrically connected (i.e., communicatively connected) to the control device 5 and is controlled to operate, thereby controlling the reagent in the test tube 21, enabling recycling of the reagent, and completing the experiment.

Further, the first reagent bottle 31 adopts a sealing structure, the first conduit 61 and the first return conduit 63 respectively extend into the first reagent bottle 31 through two conduit holes at the top of the first reagent bottle 31, and the test tube 21 and the first reagent bottle 31 form a sealed experimental environment through the first conduit 61 and the first return conduit 63.

In another variation, a plurality of first reagent bottles 31 are disposed in the first reagent compartment 3, wherein at least one first reagent bottle 31 employs a sealing structure; the pathological tissue preparation apparatus has a plurality of first tubes 61 connected from the test tubes in the tissue chamber 2 to the plurality of first reagent bottles 31, respectively, and the plurality of first tubes 61 are collected to the test tubes via a shunt capable of individually controlling the on/off of any first tube 61. When the diverter controls the opening of a certain path of first conduit 61, if the corresponding first reagent bottle 31 adopts a sealing structure, the first reagent bottle 31 and the test tube 21 form a sealed experimental environment through the corresponding first conduit 61 and the corresponding first return conduit 63; when the diverter controls one path of the first conduit to be opened, if the corresponding first reagent bottle 31 does not adopt a sealing structure, the first reagent bottle 31 is communicated with the air, and the first reagent bottle 31 and the test tube 21 form an unsealed experimental environment through the corresponding first conduit 61 and the corresponding first return conduit 63.

The operation of the pathological tissue preparation apparatus according to the present invention in an experiment (in particular, pathological tissue preparation) is explained below. Different tissue specimens need different processing environments and processing time, each tissue bin independently adopts a corresponding optimal manufacturing scheme, and control is realized through a control device. Each tissue cabin operates independently, and the temperature does not influence each other. And starting a corresponding number of tissue bins according to the number and the types of the specimens every day. When the amount of the sample is small, the reagent is not wasted; when in urgent need, the rest tissue bins can be opened at any time.

In the experimental process, after the dehydration is transparent, the water in the stock solution bath bin is discharged into a recovery bin, and the water in the 66 ℃ water bin (high-temperature water bin) is immediately injected into the solution bath bin. Meanwhile, the target temperature of the tissue bin is adjusted to 66 ℃, the temperature difference of 29 ℃ of the tissue bin is rapidly increased, the temperature is changed from 37 ℃ to 62-66 ℃, the wax is guaranteed to be soaked in time, meanwhile, the temperature is constant and accurate, and the risk of overheating is avoided. After the wax dipping is finished, the tissue bin is stopped from heating, water in the liquid bath bin is immediately drained back to the recovery bin, and room temperature water (low-temperature liquid) is injected into the liquid bath bin, so that the temperature of the tissue bin is quickly reduced to room temperature, excessive wax dipping is avoided, and the flaking quality and safety are guaranteed.

Each tissue bin operates independently, and a group of test tubes and corresponding structures such as a pump, a first catheter (or the first catheter and a second catheter) and an airway tube can be arranged in each tissue bin for pathological experiments. A plurality of test tubes and corresponding pumps, first conduits (or first and second conduits), airway tubes, and the like may also be disposed within the tissue chamber. The same tissue bin can be used for parallel pathological specimen operation. The tissue bin injects high-temperature water and low-temperature water into a liquid bath interlayer on the outer wall of the tissue bin through the high-temperature water bin and the low-temperature water bin to adjust the environmental temperature in the tissue bin, and the soaking equipment in the tissue bin is used for accelerating the temperature balance in the tissue bin. The test tube is placed in the tissue bin, and the tissue bin is connected with the first reagent bin and the second reagent bin through the first conduit and the second conduit. Paraffin and other liquid which needs to be kept at a certain temperature to ensure fluidity are placed in the first reagent bin, heat-insulating layers are arranged on each first conduit (or each first conduit and each second conduit) and each ventilation conduit which are connected with the first reagent bin, and the inner diameters of the walls of each first conduit and each ventilation conduit which are connected with the first reagent bin are larger than those of each second conduit and each ventilation conduit which are connected with the second reagent bin in an organization bin, so that the fluidity of the liquid in the first reagent bin is kept, and blockage is avoided. Each tissue cabin is provided with a temperature adjusting device for adjusting the temperature in the tissue cabin.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features. In other words, the non-conflicting portions of the above-described embodiments may be replaced or supplemented with each other to form a new embodiment.

The above embodiments only express a few embodiments of the present invention, and the description is specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.

Claims

1. A pathological tissue preparation apparatus comprising:

a tissue chamber (2) in which test tubes (21) are arranged;

a first reagent magazine (3) in which a first reagent bottle (31) is arranged;

a first conduit (61) connecting the first reagent bottle (31) to the test tube (21) in the tissue cartridge (2);

a first pump (81) arranged on the first conduit (61) for delivering the reagent in the first reagent bottle (31) into the test tube (21);

an air vent tube (71) having one end connected to the test tube (21) and the other end communicating with the outside air of the test tube; and

a control device (5) electrically connected to the first pump (81).

2. Pathological tissue preparation device according to claim 1, characterized in that one or more tube sets, each comprising one tube or a plurality of tubes connected in series, are arranged in the tissue magazine (2), wherein the first duct (61) has at one end located in the tissue magazine (2) one or more first tube-end branch ducts respectively connected to a corresponding one or more of said one or more tube sets.

3. The pathological tissue preparation apparatus according to claim 2, characterized in that it further comprises a first reagent buffer bottle (65), said first reagent buffer bottle (65) being connected to the one or more tube sets by a first top conduit, wherein the first top conduit has at one end remote from the first reagent buffer bottle (65) a respective one or more first top branch conduits connected to the one or more tube sets, respectively.

4. Pathological tissue preparation device according to claim 2, characterized in that the airway tube (71) has one or more airway branch tubes connected respectively to the one or more tube sets.

5. The pathological tissue preparation apparatus according to claim 2, characterized in that it has one or more respective airway tubes (71) connected to said one or more tube sets, respectively.

6. The pathological tissue preparation device according to claim 1, characterized in that a plurality of first reagent bottles (31) are arranged in said first reagent cartridge (3), and said first conduit (61) has a plurality of first reagent end branch conduits at one end of said first reagent cartridge (3) connected to said plurality of first reagent bottles (31), respectively.

7. The pathological tissue preparation device according to claim 1, characterized in that a plurality of first reagent bottles (31) are provided in said first reagent cartridge (3) and in that it has a plurality of first conduits (61) respectively connected from said test tubes in said tissue cartridge (2) to said plurality of first reagent bottles (31).

8. Pathological tissue preparation device according to claim 7, characterized in that the first plurality of conduits (61) converge to the test tube via a shunt.

9. Pathological tissue preparation device according to claim 4, wherein one or more of the first plurality of tube-end branch conduits, the plurality of airway branch conduits are provided with on-off valves and/or one or more of the plurality of airway branch conduits are provided with air pumps.

10. Pathological organisation making device according to claim 2, characterized in that the plurality of first tube-end branch conduits are connected to the common first conduit (61) via a multi-way valve.

11. The apparatus of claim 5, wherein one or more of the plurality of first tube-end branch conduits and the plurality of airway conduits has an on-off valve disposed thereon.

12. The pathological tissue preparation apparatus according to claim 6, wherein an on-off valve is disposed on one or more of the plurality of first reagent-end branch conduits.

13. The pathological tissue preparation apparatus according to claim 1, characterized in that it further comprises a first return conduit (63), one end of the first return conduit (63) being connected to the test tube and the other end being connected to the first reagent bottle (31).

14. The pathological tissue preparation apparatus according to claim 1, further comprising:

a second reagent magazine (4) in which a second reagent bottle (41) is arranged;

a second conduit (62) connecting the second reagent bottle (41) to the test tube (21) in the tissue cartridge (2);

a second pump (82) disposed on the second conduit (62) for delivering reagent in the second reagent bottle (41) into the test tube (21).

15. Pathological tissue preparation device according to claim 14, characterized in that one or more test tubes sets are arranged in the tissue cartridge (2), each test tube set comprising one test tube or a plurality of test tubes connected in series, wherein the first (61) and second (62) conduits converge at one end of the tissue cartridge (2) into one common conduit, which is connected to the respective one or more test tube set via one or more branch conduits.

16. The pathological tissue preparation device according to claim 14, characterized in that said tissue cartridge (2) has one or more test tubes, each test tube comprising one test tube or a plurality of test tubes connected in series; at least one test tube of the one or more tube sets receives reagent via only one inlet located at a bottom of the test tube and/or at least one test tube of the one or more tube sets discharges reagent via only one outlet located at a top of the test tube.

17. The pathological tissue preparation device according to claim 14, characterized in that one or more tube sets are arranged in the tissue cartridge (2), each tube set comprising one test tube or a plurality of test tubes connected in series, the pathological tissue preparation device further comprising a second reagent buffer bottle (66), the second reagent buffer bottle (66) being connected to the one or more tube sets by a second top conduit, wherein the second top conduit has at an end remote from the second reagent buffer bottle (66) a respective one or more second top branch conduits respectively connected to the one or more tube sets.

18. Pathological tissue preparation device according to claim 14, characterized in that an on-off valve is provided on the first (61) and/or second (62) duct and/or on the airway tube (71).

19. Pathological tissue preparation device according to claim 14, characterized in that a level detector is provided on the airway tube (71) and/or on the first tube (61) and/or on the second tube (62).

20. Pathological tissue preparation device according to claim 14, characterized in that one or more tube groups, each comprising one tube or a plurality of tubes connected in series, are arranged in the tissue cartridge (2), wherein the first duct (61) has at one end located in the tissue cartridge (2) a respective one or more first tube-end branch ducts connected respectively to said one or more tube groups, and the second duct (62) has at one end located in the tissue cartridge (2) a respective one or more second tube-end branch ducts connected respectively to said one or more tube groups.

21. Pathological tissue preparation device according to claim 14, characterized in that a plurality of first reagent bottles (31) are arranged in the first reagent cartridge (3) and the first conduit (61) has at one end located in the first reagent cartridge (3) a plurality of first reagent end branch conduits connected to the plurality of first reagent bottles (31), respectively, and/or,

a plurality of second reagent bottles (41) are provided in the second reagent magazine (4), and the second conduit (62) has a plurality of second reagent end branch conduits connected to the plurality of second reagent bottles (41), respectively, at one end located in the second reagent magazine (4).

22. The pathological tissue preparation apparatus according to claim 14, wherein a plurality of first reagent bottles (31) are provided in the first reagent cartridge (3), and the apparatus has a plurality of first conduits (61) connected to the plurality of first reagent bottles (31) from test tubes in the tissue cartridge, respectively, and,

a plurality of second reagent bottles (41) are provided in the second reagent magazine (4), and the pathological tissue preparation apparatus has a plurality of second tubes (62) connected to the plurality of second reagent bottles (41) from test tubes in the tissue magazine, respectively.

23. Pathological tissue preparation apparatus according to claim 22, characterized in that the plurality of first conduits (61) are collected via a first shunt (67) into one conduit which is connected to the test tube and/or,

the plurality of second conduits (62) are collected into one conduit connected to the test tube via a second flow splitter (68).

24. Pathological tissue preparation device according to claim 22, characterized in that the plurality of first conduits (61) and the plurality of second conduits (62) are collected into one conduit connected to the test tube via a first flow splitter (67).

25. The apparatus of claim 20, wherein one or more of the first and second plurality of test tube end branch conduits are provided with an on-off valve.

26. The pathological tissue preparation apparatus according to claim 21, wherein one or more of the first and second reagent-side branch conduits are provided with an on-off valve.

27. The pathological tissue preparation device according to claim 14, characterized in that it further comprises a first return conduit (63), said first return conduit (63) being connected at one end to said test tube and at the other end to said first reagent bottle (31); and/or the like, and/or,

the pathological tissue preparation apparatus further comprises a second return conduit (64), one end of the second return conduit (64) being connected to the test tube and the other end being connected to the second reagent bottle (41).

28. The pathological tissue preparation apparatus according to any of claims 1-27, characterized in that it further comprises a temperature regulation device and a temperature sensor for detecting the internal temperature of the tissue cartridge (2),

wherein the temperature regulating device comprises a soaking device arranged inside the tissue cabin (2), and the soaking device is used for enabling the temperatures of different positions inside the tissue cabin (2) to tend to be consistent; and/or the presence of a gas in the gas,

the temperature adjusting device comprises refrigeration equipment arranged inside the tissue bin (2), and the refrigeration equipment is used for cooling the tissue bin (2); and/or the presence of a gas in the gas,

the temperature adjusting device comprises a heating device arranged inside the tissue cabin (2), and the heating device is used for heating the tissue cabin (2).

29. The pathological tissue preparation apparatus according to any one of claims 1-27, characterized in that it further comprises a temperature regulation device comprising a liquid bath compartment disposed within the tissue compartment (2), and a temperature sensor for detecting the internal temperature of the tissue compartment (2), wherein the pathological tissue preparation apparatus further comprises a first reservoir for supplying a temperature regulation liquid having a first temperature range to the liquid bath compartment.

30. Pathological tissue preparation device according to claim 29, characterised in that the thermoregulation means comprise a soaking device arranged inside the tissue cabin (2) for bringing the temperature at different positions inside the tissue cabin (2) to unity; and/or the presence of a gas in the gas,

31. The pathological tissue preparation apparatus according to claim 29, wherein the used temperature-regulating liquid in the liquid bath compartment is returned to the first compartment directly or via a recovery compartment, wherein the recovery compartment is adapted to receive the used temperature-regulating liquid from the liquid bath compartment and to restore the temperature of the received temperature-regulating liquid to the first temperature range.

32. The apparatus according to claim 29, wherein said apparatus further comprises a second reservoir for supplying a temperature regulating liquid having a second temperature range to said bath reservoir, wherein said first reservoir and said second reservoir are connected to said bath reservoir via one inlet of said bath reservoir or are connected to said bath reservoir via two inlets of said bath reservoir, respectively.

33. The pathological tissue preparation apparatus according to claim 32, wherein the temperature regulating liquid used in the liquid bath compartment is returned to the respective first or second reservoir.

34. The apparatus according to claim 32, further comprising a recovery compartment for receiving used temperature conditioning liquid from the liquid bath compartment, restoring the temperature of the received temperature conditioning liquid to the first temperature range or the second temperature range, and returning the temperature restored temperature conditioning liquid to the respective first or second cartridge.

35. The pathological tissue preparation apparatus according to claim 32, further comprising:

a first recovery tank (8) for receiving the used temperature regulation liquid of the first temperature range from the liquid bath tank, recovering the temperature of the received temperature regulation liquid to the first temperature range, and returning the temperature-recovered temperature regulation liquid to the first storage tank; and

a second recovery tank (9) for receiving the used temperature-regulating liquid of the second temperature range from the liquid bath tank, restoring the temperature of the received temperature-regulating liquid to the second temperature range, and returning the temperature-restored temperature-regulating liquid to the second storage tank,

wherein the first recovery bin (8) and the second recovery bin (9) are communicated to the liquid bath bin via one outlet of the liquid bath bin or are respectively communicated to the liquid bath bin via two outlets of the liquid bath bin.

36. The apparatus of claim 35, wherein one or more of the piping between the first storage compartment and the liquid bath compartment, the piping between the second storage compartment and the liquid bath compartment, the piping between the liquid bath compartment and the first recovery compartment, the piping between the liquid bath compartment and the second recovery compartment, the piping between the first recovery compartment and the first storage compartment, and the piping between the second recovery compartment and the second storage compartment are provided with a switch valve and/or a pump.

37. The apparatus of claim 35, wherein one or more of the first storage chamber, the second storage chamber, the first recovery chamber and the second recovery chamber has a storage chamber temperature control module and a temperature sensor disposed therein, and the storage chamber temperature control module and the temperature sensor are electrically connected to the control device, wherein the storage chamber temperature control device comprises a heating device and/or a cooling device.

38. The pathological tissue preparation device according to claim 29, characterized in that the internal space of the tissue chamber (2) forms the liquid bath chamber and the test tubes are immersed in a temperature-regulating liquid of the liquid bath chamber.

39. Pathological tissue preparation apparatus according to claim 29, characterised in that the outer shell of the tissue cartridge (2) has spaced-apart inner and outer layers, the space defined between the inner and outer layers forming the liquid bath cartridge.

40. The pathological tissue preparation apparatus according to any one of claims 1-27, characterized in that it further comprises a temperature regulation device comprising a liquid bath compartment disposed within the tissue compartment (2), and a temperature sensor for detecting the internal temperature of the tissue compartment (2), wherein it further comprises a plurality of reservoirs for supplying temperature regulation liquid having a plurality of temperature ranges to the liquid bath compartment.

41. The apparatus of claim 40, further comprising one or more recovery bins for receiving used temperature conditioning fluid from the fluid bath bin, restoring the temperature of the received temperature conditioning fluid to one of the plurality of temperature ranges, and returning the temperature restored temperature conditioning fluid to a corresponding one of the plurality of bins.

42. Pathological tissue preparation apparatus according to claim 1, characterized in that it comprises a plurality of tissue magazines (2), a plurality of first ducts (61) and a plurality of aeration ducts (71), wherein each tissue magazine (2) has one or more test tubes arranged therein, and wherein the one or more test tubes in each tissue magazine (2) are connected to the first reagent bottle (31) by means of the corresponding first duct (61) and are in communication with the outside air of the test tube by means of a corresponding one of the plurality of aeration ducts (71).

43. Pathological tissue preparation apparatus according to claim 14, characterized in that it comprises a plurality of tissue silos (2), a plurality of first conduits (61), a plurality of second conduits (62) and a plurality of vent conduits (71), wherein one or more test tubes are arranged in each tissue silo (2), and wherein the one or more test tubes in each tissue silo (2) are connected to the first reagent bottle (31) by means of the corresponding first conduit (61), to the second reagent bottle (41) by means of the corresponding second conduit (62) and are in communication with the outside air of the test tubes by means of a corresponding one of the plurality of vent conduits (71).

44. The pathological tissue preparation device according to claim 42 or 43, characterized in that said plurality of tissue bins (2) have a corresponding plurality of liquid bath bins, and in that it further comprises a plurality of reservoirs for supplying temperature regulating liquid having a plurality of temperature ranges to said plurality of liquid bath bins.

45. The apparatus of claim 44, further comprising one or more recovery bins for receiving used temperature conditioning fluid from each of said plurality of fluid bath bins, restoring the temperature of the received temperature conditioning fluid to one of said plurality of temperature ranges, and returning the temperature restored temperature conditioning fluid to a corresponding one of said plurality of bins.

46. The pathological tissue preparation apparatus according to any of claims 14 to 27, wherein the first catheter has a larger tube inner diameter than the second catheter.

47. The apparatus of any one of claims 14-27, wherein the outer wall of one or more of the first conduit, the second conduit, and the airway tube is provided with an insulating layer.

48. Pathological tissue preparation apparatus according to any of claims 14 to 27, characterized in that a temperature control device is arranged in the first reagent chamber (3) and/or in the second reagent chamber (4), said temperature control device being electrically connected to the control device (5).

49. Pathological tissue preparation device according to claim 18, wherein the opening of the on-off valve is adjustable and/or the flow rate of the first and/or second pump is adjustable.

50. Pathological tissue preparation device according to any of claims 1 to 27, characterized in that an air pump (85) is arranged on the airway tube (71), the air pump (85) being electrically connected to the control means (5).

51. The pathological tissue preparation apparatus according to claim 1, characterized in that it further comprises a return conduit connected at one end to the test tube (21) and at the other end to the first reagent bottle (31).

52. A pathological tissue preparation apparatus, comprising:

a tissue chamber (2) in which test tubes (21) are arranged;

a first reagent magazine (3) in which a first reagent bottle (31) is arranged;

a first pump (81) arranged on the first conduit (61) for transferring the reagent in the first reagent bottle (31) into the test tube (21);

a first return conduit (63) connected at one end to said test tube (21) and at the other end to said first reagent bottle (31); and

a control device (5) electrically connected to the first pump (81).

53. Pathological tissue preparation apparatus according to claim 52, characterized in that the first reagent bottle (31) is a sealed structure, the first conduit (61) and the first return conduit (63) extend into the first reagent bottle (31), and the test tube (21) and the first reagent bottle (31) form a sealed experimental environment via the first conduit (61) and the first return conduit (63).

54. The pathological tissue preparation apparatus according to claim 52, further comprising:

a second reagent magazine (4) in which a second reagent bottle (41) is disposed;

a second return conduit (64) connected at one end to said test tube (21) and at the other end to said second reagent bottle (41);

55. Pathological tissue preparation apparatus according to claim 54, characterized in that the first reagent bottle (31) is a sealed structure, the first conduit (61) and the first return conduit (63) extend into the first reagent bottle (31), the second pump (82) is not operated when the first pump (81) is operated, and the test tube (21) and the first reagent bottle (31) form a sealed experimental environment through the first conduit (61) and the first return conduit (63); and/or the like, and/or,

the second reagent bottle (41) adopts a sealing structure, the second conduit (62) and the second return conduit (64) extend into the second reagent bottle (41), when the second pump (82) works, the first pump (81) does not work, and the test tube (21) and the second reagent bottle (41) form a flowable sealed experimental environment through the second conduit (62) and the second return conduit (64).

56. The pathological tissue preparation device according to claim 52, wherein a plurality of first reagent bottles (31) are disposed in said first reagent chamber (3), wherein at least one first reagent bottle is a sealed structure;

the pathological tissue preparation device is provided with a plurality of first conduits (61) which are respectively connected to the plurality of first reagent bottles (31) from the test tubes (21) in the tissue bin (2), the plurality of first conduits (61) are collected to the test tubes (21) through a flow divider, the flow divider can independently control the on-off of any first conduit (61), the test tubes (21) are distributed to a plurality of first return conduits (63) through return flow dividers, and the return flow dividers can independently control the on-off of any first return conduit (63).