CN115634726B

CN115634726B - Pathological tissue making equipment

Info

Publication number: CN115634726B
Application number: CN202211660157.8A
Authority: CN
Inventors: 请求不公布姓名
Original assignee: Tianjin Mingrui Pharmaceutical Technology Co ltd
Current assignee: Tianjin Mingrui Pharmaceutical Technology Co ltd
Priority date: 2022-12-23
Filing date: 2022-12-23
Publication date: 2023-10-31
Anticipated expiration: 2042-12-23
Also published as: CN115634726A

Abstract

The application discloses a pathological tissue making device, which is characterized by comprising: a tissue compartment in which test tubes are disposed; a first reagent chamber in which a first reagent bottle is disposed; a first conduit connecting the first reagent bottle to the test tube in the tissue cartridge; a first pump provided on the first conduit for delivering the reagent in the first reagent bottle into the test tube; one end of the ventilation catheter is connected with the test tube, and the other end of the ventilation catheter is communicated with the outside air of the test tube; and a control device electrically connected to the first pump.

Description

Pathological tissue making equipment

Technical Field

The application relates to pathological tissue making equipment, in particular to an incubator for pathological tissue making.

Background

The pathological tissue preparation technology is widely applied to medical clinic and scientific research. Pathological tissue preparation is usually carried out by preparing a tissue sample into a tissue wax block through dehydration, transparency, wax dipping and other treatments. Hospitals need to examine a large number of pathological tissues every day. The time and quality of pathological tissue production is related to temperature, pathological tissue type, and pathological tissue size. The tissue preparation has temperature requirements, and the too high or the too low temperature can influence the quality and the safety of the tablet preparation.

Currently, pathological tissue preparation is typically performed in high volume devices. In such devices, batches of pathological tissue are fabricated simultaneously, there is a lack of flexibility, and the space occupied by pathological tissue fabrication is excessive. Moreover, no matter how many pathological tissues need to be prepared, a large amount of reagents need to be used and consumed in one experiment, so that the preparation cost of pathological specimens is high. In addition, pathological tissues with different types and sizes are placed in the same experimental environment to manufacture the pathological tissues, so that the quality of pathological specimens cannot be accurately controlled. The pathological tissue preparation process is basically semiautomatic, and the pathological preparation process still needs to be carried out manually, so that a great deal of manpower and time are occupied, and the pathological preparation efficiency is low. Moreover, the number of pathology tests increases year by year, and higher demands are placed on the number and time of tissue preparation. Therefore, there is an urgent need to improve flexibility, degree of automation, efficiency and quality of fabrication of pathological tissue.

Disclosure of Invention

In order to solve the above-mentioned problems of the prior art, the present invention provides a pathological tissue producing apparatus, characterized in that the pathological tissue producing apparatus includes: a tissue compartment in which test tubes are disposed; a first reagent chamber in which a first reagent bottle is disposed; a first conduit connecting the first reagent bottle to the test tube in the tissue cartridge; a first pump provided on the first conduit for delivering the reagent in the first reagent bottle into the test tube; one end of the ventilation catheter is connected with the test tube, and the other end of the ventilation catheter is communicated with the outside air of the test tube; and a control device electrically connected to the first pump.

In particular, the pathological tissue making apparatus is an incubator. Preferably, the first conduit is connected to the test tube at the bottom of the test tube and/or the airway tube is connected to the test tube at the top of the test tube.

Preferably, one or more tube sets are provided in the tissue magazine, each tube set comprising one tube or a plurality of tubes connected in series, wherein the first conduit has at one end of the tissue magazine a respective one or more first tube-end branch conduits connected to the one or more tube sets, respectively.

Preferably, the pathological tissue producing device further comprises a first reagent temporary storage bottle connected to the one or more test tube groups by a first top conduit, wherein the first top conduit has at an end remote from the first reagent temporary storage bottle a respective one or more first top branch conduits connected to the one or more test tube groups, respectively. Advantageously, the first reagent temporary storage bottle is located in the tissue compartment or the first reagent compartment. The first reagent temporary storage bottle is configured to extract a reagent from the test tube and temporarily store the extracted reagent therein, or to push the reagent temporarily stored therein into the test tube, for example, by a pump, thereby realizing recycling of the reagent. Preferably, the first reagent temporary storage bottle may be located on the airway tube. That is, the first top conduit is a section of the airway tube, and the first reagent temporary storage bottle may be formed by thickening a portion of the exhaust conduit. At this time, the exhaust duct simultaneously plays a role of temporary storage reagent and occupies only one port at the top of the test tube (when the exhaust duct and the first reagent temporary storage bottle are separate components, they are connected to the test tube through two ports).

Advantageously, the ventilation catheter is connected to the one or more test tube sets from a catheter having one or more ventilation branch catheters, respectively. Alternatively, the pathological tissue making device has a respective one or more ventilation catheters connected to the one or more sets of tubes, respectively.

In a preferred embodiment, a plurality of test tubes are provided in the tissue magazine, and the first catheter has a plurality of first test tube end branch catheters at one end of the tissue magazine connected to the plurality of test tubes, respectively. Preferably, the airway tube has a plurality of airway branch tubes at one end of the tissue cartridge connected to the plurality of tubes, respectively. Alternatively, the pathological tissue making apparatus has a plurality of ventilation catheters respectively connected to the plurality of test tubes.

In one variant, a plurality of tube sets are provided in the tissue magazine, each tube set comprising one tube or a plurality of tubes connected in series, wherein the first conduit has a plurality of first tube-end branch conduits at one end of the tissue magazine connected to the plurality of tube sets, respectively. Preferably, the airway tube has a plurality of airway branch tubes at one end of the tissue cartridge connected to the plurality of tube sets, respectively. Alternatively, the pathological tissue producing apparatus has a plurality of ventilation catheters respectively connected to the plurality of test tube groups.

In particular, when the tube group includes a plurality of tubes connected in series, the plurality of tubes are arranged in the tissue magazine in the up-down direction. Preferably, the first tube-end branch conduit is connected to a bottommost conduit of the plurality of tubes arranged up and down, and/or the ventilation branch conduit or the ventilation conduit is connected to a topmost conduit of the plurality of tubes arranged up and down.

In one embodiment, a plurality of first reagent bottles are disposed in the first reagent cartridge, and the first conduit has a plurality of first reagent-end branch conduits at one end of the first reagent cartridge connected to the plurality of first reagent bottles, respectively.

In one embodiment, a plurality of first reagent bottles are disposed in the first reagent cartridge, and the pathological tissue producing device has a plurality of first conduits connected to the plurality of first reagent bottles, respectively, from the test tubes in the tissue cartridge. Advantageously, said plurality of first conduits is collected to said test tube via a shunt. Preferably, the diverter is a one-to-many pipe joint. Preferably, the shunt is integrally formed with the plurality of first conduits. Alternatively, the shunt is formed separately from the plurality of first conduits.

Preferably, one or more of the plurality of first tube-end branch ducts and the plurality of ventilation branch ducts is/are provided with an on-off valve. Alternatively, the plurality of first tube-end branch conduits are connected to a common said first conduit via a multi-way valve. Advantageously, an air pump is provided on one or more of the plurality of ventilation branch ducts.

Preferably, one or more of the plurality of first tube-end branch ducts and the plurality of ventilation ducts is/are provided with an on-off valve.

Preferably, one or more of the plurality of first reagent-side branch conduits is provided with an on-off valve.

Preferably, the pathological tissue producing device further comprises a first return conduit, one end of which is connected to the test tube and the other end of which is connected to the first reagent bottle. The first return conduit is configured to return the reagent in the test tube to the first reagent bottle, thereby enabling recycling of the reagent.

Preferably, the pathological tissue producing apparatus further comprises a return conduit having one end connected to the test tube and the other end connected to the first reagent bottle.

In an advantageous embodiment, the pathological tissue making apparatus further comprises: a second reagent chamber in which a second reagent bottle is disposed; a second conduit connecting the second reagent bottle to the test tube in the tissue cartridge; a second pump is provided on the second conduit for delivering the reagent in the second reagent bottle into the test tube.

Preferably, one or more tube sets are provided in the tissue magazine, each tube set comprising one tube or a plurality of tubes connected in series, wherein the first and second conduits merge at one end at the tissue magazine into one common conduit, which is connected to the respective one or more tube sets via one or more branch conduits.

Preferably, one or more groups of test tubes are provided in the tissue magazine, each group of test tubes comprising one test tube or a plurality of test tubes connected in series, at least one of the one or more groups of test tubes receiving reagent via only one inlet at the bottom of the test tube and/or at least one of the one or more groups of test tubes expelling reagent via only one outlet at the top of the test tube.

Preferably, one or more tube sets are provided in the tissue magazine, each tube set comprising one tube or a plurality of tubes connected in series, the pathological tissue producing device further comprising a second reagent temporary storage bottle connected to the one or more tube sets by a second top conduit, wherein the second top conduit has at an end remote from the second reagent temporary storage bottle a respective one or more second top branch conduits connected to the one or more tube sets, respectively. Advantageously, the second reagent temporary storage bottle is located in the tissue compartment or the second reagent compartment. The second reagent temporary storage bottle is configured to extract a reagent from the test tube and temporarily store the extracted reagent therein, or push the reagent temporarily stored therein into the test tube, for example, by a pump, thereby realizing recycling of the reagent. When the first reagent temporary storage bottle and the second reagent temporary storage bottle exist at the same time, the first reagent temporary storage bottle and the second reagent temporary storage bottle are connected to the test tube through two ports, so that temporary storage of different reagents can be realized. One of the first reagent buffer bottle and the second reagent buffer bottle may be located at the vent conduit and occupy the same port located at the top of the test tube as the vent conduit. Preferably, an on-off valve is arranged on the first conduit and/or the second conduit and/or the ventilation conduit.

Preferably, a liquid level detector is arranged on the ventilation catheter and/or the first catheter and/or the second catheter.

Preferably, the pathological tissue producing apparatus further comprises: a plurality of reagent chambers, each of which is provided with one or a plurality of reagent bottles; a reagent cartridge catheter connecting one or more reagent bottles in the reagent cartridge to the test tube in the tissue cartridge, wherein the number of reagent cartridge catheters is equal to or greater than the number of reagent cartridges, such that each reagent cartridge is configured with at least one reagent cartridge catheter; a reagent cartridge catheter pump disposed on the reagent cartridge catheter for delivering reagent in one or more reagent bottles in the reagent cartridge into the test tube. Preferably, a temperature regulating device is provided in the reagent compartment for maintaining the temperature in the reagent compartment. The maintenance temperatures set in the plurality of reagent chambers may be the same or different.

Preferably, the pathological tissue producing apparatus further comprises: a plurality of reagent temporary storage bottles for temporarily storing the reagent flowing out from the top of the test tube; a plurality of reagent temporary storage bottle conduits for connecting the test tube and a plurality of reagent temporary storage bottles. Preferably, the reagent temporary storage bottle is arranged in the tissue warehouse, and the working temperature of the reagent temporary storage bottle changes with the temperature of the tissue warehouse. Preferably, a portion of the reagent temporary storage bottles are disposed in the tissue compartment and a portion of the reagent temporary storage bottles are disposed in the reagent compartment. Preferably, the number of the reagent temporary storage bottles is the same as the number of the reagent bins, and the reagent temporary storage bottles are arranged in the corresponding reagent bins. In the experimental process, the reagents flowing in each reagent temporary storage bottle when the reagents are temporarily stored are the reagents stored in the corresponding reagent bin.

Preferably, the pathological tissue producing apparatus further comprises: the reagent in the test tube flows into the corresponding reagent bottle, one end of the return tube is connected with the top of the test tube in the tissue bin, the other end of the return tube is connected with the reagent bottle in the reagent bin, the depth of the return tube extending into the test tube can be adjusted according to the actual use requirement, and likewise, the depth of the return tube extending into the reagent bottle can also be adjusted according to the actual use requirement.

Preferably, a plurality of return lines are each provided with an on-off valve, which is connected in communication with the control device.

Preferably, when a plurality of return ducts are simultaneously connected to one test tube, the connection may be made using a multiple-input-one-output structure such as a multi-way valve. Similarly, when a plurality of return pipes are connected to one reagent bottle at the same time, the connection may be performed by a multiple-input-one-output structure such as a multi-way valve. Further, when the multiple return pipes are matched with the multiple reagent bottles, two ends of the multiple return pipes can be connected by adopting a multi-way valve and other multi-input and one-output or multi-input and multi-output structures.

Preferably, the pathological tissue making device further comprises a housing, and the tissue bin, the first reagent bin and the second reagent bin are all arranged in the housing.

Preferably, one or more tube sets are provided in the tissue magazine, each tube set comprising one tube or a plurality of tubes connected in series, wherein the first conduit has at one end of the tissue magazine a respective one or more first tube end branch conduits connected to the one or more tube sets, respectively, and the second conduit has at one end of the tissue magazine a respective one or more second tube end branch conduits connected to the one or more tube sets, respectively.

Preferably, a plurality of first reagent bottles are provided in the first reagent cartridge, and the first conduit has a plurality of first reagent end branch conduits at one end of the first reagent cartridge connected to the plurality of first reagent bottles, respectively, and a plurality of second reagent bottles are provided in the second reagent cartridge, and the second conduit has a plurality of second reagent end branch conduits at one end of the second reagent cartridge connected to the plurality of second reagent bottles, respectively.

Preferably, a plurality of first reagent bottles are provided in the first reagent cartridge, and the pathological tissue producing apparatus has a plurality of first catheters respectively connected to the plurality of first reagent bottles from test tubes in the tissue cartridge, and a plurality of second reagent bottles are provided in the second reagent cartridge, and the pathological tissue producing apparatus has a plurality of second catheters respectively connected to the plurality of second reagent bottles from test tubes in the tissue cartridge. Advantageously, the plurality of first conduits are collected via a first flow divider as one conduit connected to the test tube and/or the plurality of second conduits are collected via a second flow divider as one conduit connected to the test tube. Alternatively, the plurality of first conduits and the plurality of second conduits are collected via a first shunt as one conduit connected to the test tube. Preferably, the first splitter and/or the second splitter is a one-to-many pipe joint. Preferably, the first flow splitter is integrally formed with the plurality of first conduits and/or the second flow splitter is integrally formed with the plurality of second conduits. Alternatively, the first shunt is formed separately from the plurality of first conduits and/or the second shunt is formed separately from the plurality of second conduits.

Preferably, one or more of the plurality of first test tube end branch ducts and the plurality of second test tube end branch ducts is/are provided with an on-off valve. Additionally or alternatively, a pump is disposed on one or more of the plurality of first tube-end branch conduits, the plurality of second tube-end branch conduits.

Preferably, one or more of the plurality of first reagent-end branch ducts and the plurality of second reagent-end branch ducts is provided with an on-off valve.

Advantageously, the pathological tissue making device further comprises a second return conduit, one end of which is connected to the test tube and the other end of which is connected to the second reagent bottle. The second return conduit is configured to return the reagent in the test tube to the second reagent bottle, thereby enabling recycling of the reagent.

In a preferred embodiment, the pathological tissue making device comprises a plurality of reagent chambers, wherein one or more reagent bottles are stored in the plurality of reagent chambers, the reagent bottles provide reagents for the test tubes in the tissue chambers, and the reagent bottles and the test tubes in the tissue chambers can be connected through a conduit or can be connected through a branch of the reagent conduit in a manner of combining with the conduit.

Preferably, one or more of the plurality of reagent chambers comprises a temperature control device, the temperature control device maintains the temperature in the reagent chamber at a preset temperature, the preset temperature is adjustable, and the preset temperatures of the plurality of reagent chambers can be the same or different. Preferably, the temperature control device may be a temperature detection device or a temperature adjustment device, where the temperature adjustment device may be a combination of a heating device and a passive cooling device (e.g. a fan), a combination of a heating device and an active cooling device (e.g. a semiconductor refrigeration sheet, a refrigeration system including a compressor and a condenser), or a combination of a heating device, a passive cooling device and an active cooling device.

In a preferred embodiment, the pathological tissue making device further comprises a temperature regulating device and a temperature sensor for detecting the internal temperature of the tissue cabin, wherein the temperature regulating device comprises a soaking device arranged inside the tissue cabin, and the soaking device is used for enabling the temperatures at different positions inside the tissue cabin to be consistent; and/or the temperature regulating device comprises a refrigerating device arranged inside the tissue cabin, wherein the refrigerating device is used for cooling the tissue cabin; and/or the temperature regulating device comprises a heating device arranged inside the tissue cabin, wherein the heating device is used for heating the tissue cabin. Preferably, the heating device is one or more of an electric heating wire and a heating bulb, and the refrigerating device is one or more of a semiconductor refrigerating sheet, a radiator fan, a heat exchanger and a liquid cooling device.

In another preferred embodiment, the pathological tissue making apparatus further comprises a temperature regulating device comprising a liquid bath cartridge arranged within the tissue cartridge and a temperature sensor for detecting the internal temperature of the tissue cartridge, wherein the pathological tissue making apparatus further comprises a first reservoir for supplying a temperature regulating liquid having a first temperature range to the liquid bath cartridge. Advantageously, the temperature regulating device comprises soaking equipment arranged inside the tissue warehouse, wherein the soaking equipment is used for enabling the temperatures at different positions inside the tissue warehouse to be consistent; and/or the temperature regulating device comprises a refrigerating device arranged inside the tissue cabin, wherein the refrigerating device is used for cooling the tissue cabin; and/or the temperature regulating device comprises a heating device arranged inside the tissue cabin, wherein the heating device is used for heating the tissue cabin. Advantageously, the used temperature regulating liquid in the liquid bath compartment is returned directly to the first storage compartment or via a recovery compartment for receiving the used temperature regulating liquid from the liquid bath compartment and restoring the temperature of the received temperature regulating liquid to the first temperature range.

Advantageously, the pathological tissue producing device further comprises a second reservoir for supplying a temperature regulating liquid having a second temperature range to the liquid bath reservoir, wherein the first reservoir and the second reservoir are in communication with the liquid bath reservoir via one inlet of the liquid bath reservoir or with the liquid bath reservoir via two inlets of the liquid bath reservoir, respectively. Preferably, the first temperature range is 62-66 ℃, and/or the second temperature range is 20-45 ℃. In particular, the second temperature range is room temperature. Advantageously, the temperature regulating liquid used in the liquid bath compartment is returned to the respective first or second storage compartment.

Preferably, the pathological tissue making apparatus further comprises a recovery bin for receiving used temperature regulating liquid from the liquid bath bin, recovering the temperature of the received temperature regulating liquid to the first temperature range or the second temperature range, and returning the temperature recovered temperature regulating liquid to the respective first or second bin. Advantageously, on one or more of the lines between the first and the liquid bath, between the second and the liquid bath, between the liquid bath and the recovery tank, between the recovery tank and the first tank and between the recovery tank and the second tank a switching valve and/or a pump is provided.

Alternatively, the pathological tissue producing apparatus further includes: a first recovery bin for receiving used temperature regulating liquid of the first temperature range from the liquid bath bin, recovering the temperature of the received temperature regulating liquid to the first temperature range, and returning the temperature recovered temperature regulating liquid to the first storage bin; and a second recovery bin for receiving the used temperature-regulated liquid of the second temperature range from the liquid bath bin, recovering the temperature of the received temperature-regulated liquid to the second temperature range, and returning the temperature-recovered temperature-regulated liquid to the second storage bin, wherein the first recovery bin and the second recovery bin are communicated to the liquid bath bin via one outlet of the liquid bath bin or are communicated to the liquid bath bin via two outlets of the liquid bath bin, respectively. Advantageously, a switching valve and/or a pump is provided on one or more of the piping between the first reservoir and the liquid bath reservoir, the piping between the second reservoir and the liquid bath reservoir, the piping between the liquid bath reservoir and the first recovery reservoir, the piping between the liquid bath reservoir and the second recovery reservoir, the piping between the first recovery reservoir and the first reservoir, and the piping between the second recovery reservoir and the second reservoir. Preferably, a bin temperature control module and a temperature sensor are arranged in one or more of the first bin, the second bin, the first recovery bin and the second recovery bin, and the bin temperature control module and the temperature sensor are electrically connected with the control device, wherein the bin temperature control device comprises a heating device and/or a cooling device. Advantageously, the heating device is one or more of a heating wire, a heating bulb, a temperature equalizing turbine, and a temperature equalizing fan. Advantageously, the cooling device is one or more of a cooling fin, a radiator fan, a heat exchanger, a liquid cooling device, a temperature equalizing turbine, and a temperature equalizing fan.

Advantageously, the recovery bin is connected to the first and second bins by two water return pumps, respectively, wherein the water return pumps are electrically connected to the control device.

Advantageously, the first storage compartment is connected to the first recovery compartment by a first water return pump, and the second storage compartment is connected to the second recovery compartment by a second water return pump, wherein the first water return pump and the second water return pump are electrically connected to the control device.

Advantageously, the internal space of the tissue compartment forms the liquid bath compartment and the test tube is immersed in the temperature regulating liquid of the liquid bath compartment. Alternatively, the housing of the tissue cartridge has spaced apart inner and outer layers, with a space defined between the inner and outer layers forming the liquid bath cartridge. Advantageously, the temperature regulating liquid is water and the liquid bath compartment is a water bath compartment.

Advantageously, the pathological tissue producing apparatus further comprises a temperature regulating device comprising a liquid bath cartridge arranged within the tissue cartridge and a temperature sensor for detecting the internal temperature of the tissue cartridge, wherein the pathological tissue producing apparatus further comprises a plurality of reservoirs for supplying the liquid bath cartridge with a temperature regulating liquid having a plurality of temperature ranges. Advantageously, the pathological tissue producing device further comprises a plurality of recovery bins for receiving used temperature regulating fluid from the fluid bath bin, recovering the temperature of the received temperature regulating fluid to one of the plurality of temperature ranges, and returning the temperature recovered temperature regulating fluid to a respective one of the plurality of bins.

Preferably, the plurality of temperature ranges includes three temperature ranges of 60-66 ℃,40-50 ℃,20-30 ℃, respectively.

In another preferred embodiment, the pathological tissue making apparatus comprises a plurality of tissue bins, a plurality of first catheters and a plurality of ventilation catheters, wherein one or more test tubes are provided in each tissue bin, and wherein the one or more test tubes in each tissue bin are connected to the first reagent bottle by the corresponding first catheter and are in air communication with the outside of the test tubes by a corresponding one of the plurality of ventilation catheters.

In yet another preferred embodiment, the pathological tissue making apparatus comprises a plurality of tissue bins, a plurality of first catheters, a plurality of second catheters, and a plurality of ventilation catheters, wherein one or more test tubes are disposed in each tissue bin, and wherein the one or more test tubes in each tissue bin are connected to the first reagent bottle by the corresponding first catheter, to the second reagent bottle by the corresponding second catheter, and are in air communication with the outside of the test tubes by a corresponding one of the plurality of ventilation catheters.

Preferably, the plurality of tissue bins has a corresponding plurality of liquid bath bins, and the pathological tissue making apparatus further comprises a plurality of reservoirs for supplying temperature regulating liquid having a plurality of temperature ranges to the plurality of liquid bath bins. Preferably, the pathological tissue producing apparatus further comprises one or more recovery bins for receiving used temperature regulating fluid from each of the plurality of fluid bath bins, recovering the temperature of the received temperature regulating fluid to one of the plurality of temperature ranges, and returning the temperature recovered temperature regulating fluid to a corresponding one of the plurality of bins.

Preferably, the first conduit has a tube inner diameter greater than a tube inner diameter of the second conduit. Advantageously or alternatively, the first conduit and the airway tube have the same tube inner diameter. Advantageously, the outer wall of one or more of the first conduit, the second conduit, the airway tube is provided with an insulating layer.

Advantageously, a temperature control device is arranged in the first reagent compartment and/or the second reagent compartment, and the temperature control device is electrically connected with the control device. Advantageously, the on-off valve is a solenoid valve.

Advantageously, the opening degree of the on-off valve can be adjusted. Additionally or alternatively, the flow rate of the first pump and/or the second pump can be adjusted.

Advantageously, an air pump is provided on the ventilation duct, said air pump being electrically connected to the control device. The air pump has the functions of pressurizing and depressurizing. Advantageously, the flow rate of the air pump is adjustable.

Preferably, the liquid bath bin is provided with an air outlet, the air outlet is connected with an air outlet switch valve or an air pump, and the air outlet switch valve or the air pump is electrically connected with the control device.

Preferably, all or part of the on-off valves are solenoid valves. Advantageously, the full or partial on-off valve is an on-off valve of adjustable opening magnitude.

The application provides a pathological tissue making device, which is characterized by comprising: a tissue compartment in which test tubes are disposed; a first reagent chamber in which a first reagent bottle is disposed; a first conduit connecting the first reagent bottle to the test tube in the tissue cartridge; a first pump provided on the first conduit for delivering the reagent in the first reagent bottle into the test tube; a first return conduit having one end connected to the test tube and the other end connected to the first reagent bottle; and a control device electrically connected to the first pump.

Preferably, the first reagent bottle adopts a sealing structure, the first guide pipe and the first return guide pipe extend into the first reagent bottle, and the test tube and the first reagent bottle form a sealed experimental environment through the first guide pipe and the first return guide pipe.

Preferably, the pathological tissue producing apparatus further comprises: a second reagent chamber in which a second reagent bottle is disposed; a second conduit connecting the second reagent bottle to the test tube in the tissue cartridge; a second return conduit having one end connected to the test tube and the other end connected to the second reagent bottle; a second pump is provided on the second conduit for delivering the reagent in the second reagent bottle into the test tube.

Preferably, the first reagent bottle adopts a sealing structure, the first conduit and the first return conduit extend into the first reagent bottle, when the first pump works, the second pump does not work, and the test tube and the first reagent bottle form a circulated sealing experiment environment through the first conduit and the first return conduit; and/or, the second reagent bottle adopts a sealing structure, the second guide pipe and the second return guide pipe extend into the second reagent bottle, when the second pump works, the first pump does not work, and the test tube and the second reagent bottle form a circulated sealing experimental environment through the second guide pipe and the second return guide pipe.

Preferably, a plurality of first reagent bottles are arranged in the first reagent bin, wherein at least one first reagent bottle adopts a sealing structure; the pathological tissue making device is provided with a plurality of first catheters which are respectively connected to the plurality of first reagent bottles from the test tubes in the tissue bin, the plurality of first catheters are converged to the test tubes through a shunt, and the shunt can independently control the on-off of any first catheter; the test tubes are dispersed to the plurality of first return conduits via a return diverter that is capable of individually controlling the on-off of any of the first return conduits.

The pathological tissue producing device according to the invention has some or all of the following beneficial effects:

1. in the invention, the first pump regulates the entry and exit of the reagent, automatically supplies the reagent into the test tube of the tissue warehouse, establishes the experimental environment and completes the experiment. The reagent amount of the reagent required in the test tube is small, the experimental environment is small, the experiment is carried out in the small experimental environment, the reagent amount required is greatly reduced, and the reagent amount can be controlled through the first pump. In one case, the first pump can control the entry and exit of the reagent, realize the transition of the experimental environment, and control the time of infiltrating the reagent.

2. The temperature in the tissue bin is monitored by the temperature sensor, and the conversion of different experimental environments is realized by different reagents in one reagent bin or in a plurality of reagent bins. In one case, the automatic dehydration and/or transparency and/or wax dipping treatment and/or wax block manufacturing process can be realized through the control device, so that the degree of automation of pathological tissues is improved, and meanwhile, the manufacturing quality is improved. Under one condition, the air pump is matched with the pumps, so that the inlet and outlet speeds of the reagents are accelerated, the reagents are returned in the original path, the reagents can be repeatedly utilized, the reagents are saved, and the experimental efficiency is improved.

3. The tissue bin is connected with the reagent bottles in the two reagent bins through the guide pipe and the switch valve, and the communication between the reagent bottles and the tissue bin is controlled through the switch valve, so that the liquid in the reagent bottles can be conveniently selected during the control experiment. The opening and closing valve can be adjusted, so that the flow rate of liquid in the guide pipe can be adjusted. In addition, since the pump speeds of the first pump and the second pump are adjustable, the flow rate of the liquid in the conduit can also be adjusted.

4. The air pump is arranged, the pressure in the test tube is controlled by the air pump so as to form negative pressure or high pressure in the test tube, so that reagents in the reagent bottle can enter the test tube or can return to the reagent bottle, the reagents can circularly flow up and down in the test tube, a sample is manufactured in fluid, the quality and the efficiency are improved, and the repeated utilization of the reagents is realized.

5. The plurality of pumps, the first guide pipe (or the first guide pipe and the second guide pipe) and the ventilation guide pipe are arranged in the same tissue bin, so that a plurality of groups of experimental environments (a plurality of test tubes/a plurality of groups of test tube strings) are arranged in the same tissue bin, a large number of pathological experiments of the same type can be simultaneously performed, and the pathological experimental efficiency is improved. The pumps can be independently controlled by the control unit, and the pathological experiments are carried out at the same temperature, but when the steps of the pathological experiments at the same environmental temperature are different, the pathological experiments can be carried out in the same tissue bin, so that the pathological experiment efficiency is improved, and the diversity of the pathological experiments in the same tissue bin is increased.

6. The scheme of the plurality of tissue bins can lead different pathological tissues to respectively carry out automatic dehydration and/or transparent and/or wax dipping treatment processes in the plurality of bins, each tissue bin uses corresponding manufacturing parameters, controls the environment in each tissue bin and controls the inflow and outflow of reagents through the control device, and processes the plurality of pathological tissues in parallel, so that the pathological tissues are manufactured individually, the manufacturing quality is improved, and the efficiency of pathological tissue treatment is improved. Thus, the high customization of pathological tissue preparation can be realized, and the preparation time is flexible.

7. In the scheme of the first storage bin and the recovery bin, the liquid bath bin shortens the temperature rise time of the tissue bin/the liquid bath bin, and the heating equipment further shortens the temperature rise time of the tissue bin, improves the manufacturing quality and ensures the manufacturing safety. The low-temperature water is arranged, and the liquid bath bin and the recovery bin are combined, so that the cooling time in the tissue bin is shortened, and the working efficiency is improved. The refrigerating equipment is arranged, so that the cooling speed is increased when the tissue warehouse is cooled.

8. Temperature control equipment is arranged in the first storage bin, the second storage bin, the recovery bin, the liquid bath bin, the first reagent bin and the second reagent bin, so that experimental environments are conveniently controlled, and temperature preparation is conveniently carried out on different experimental environments.

Drawings

Fig. 1 shows a schematic view of a first embodiment of a pathological tissue producing device according to the invention;

fig. 2 shows a schematic view of a second embodiment of a pathological tissue producing device according to the invention and variants thereof;

fig. 3, 4 and 5 show schematic views of further variants of a second embodiment of a pathological tissue producing device according to the invention, wherein the control means are not shown;

fig. 6 shows a schematic view of a third embodiment of a pathological tissue producing device according to the invention;

Fig. 7 and 8 show schematic views of different variants of a third embodiment of a pathological tissue producing device according to the invention, wherein the control means are not shown;

fig. 9 shows a schematic view of a fourth embodiment of a pathological tissue producing device according to the invention, wherein the control means are not shown;

fig. 10 shows a schematic view of a fifth embodiment of a pathological tissue producing device according to the invention, wherein the control means and the reagent cartridge are not shown;

fig. 11 shows a schematic view of a sixth embodiment of a pathological tissue producing device according to the invention, wherein the control means are not shown;

FIG. 12 shows a seventh embodiment of a pathological tissue producing apparatus according to the present invention, wherein only the tissue and reagent cartridges are shown;

fig. 13 shows a schematic view of an eighth embodiment of a pathological tissue producing device according to the present invention.

Detailed Description

In order that the invention may be readily understood, a more complete description of the invention will be rendered by reference to the appended drawings. The drawings illustrate preferred embodiments of the invention. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.

It should be understood that the crisscrossed conduits, lines or lines shown in the various figures are all excluded from being connected or communicating with each other.

Fig. 1 shows a schematic view of a first embodiment of a pathological tissue producing device according to the invention. The pathological tissue making apparatus, in particular an incubator, comprises a tissue compartment 2, a first reagent compartment 3 and a control device 5. A test tube 21 is placed in the tissue magazine 2, and an experimental specimen 10 is placed in the test tube 21. The test tube may be a disposable replaceable consumable. The actual product shape of the tube may be similar to drip chambers used in intravenous sets. A first reagent bottle 31 is placed in the first reagent vessel 3, and the first reagent bottle 31 is connected to the test tube 21 in the tissue vessel 2 via a first conduit 61. A first pump 81 is provided in the first conduit 61, and the liquid (reagent) in the first reagent bottle 31 is transferred to the test tube 21 by the first pump 81. The first pump 81 may return the reagent in the test tube 21 to the first reagent bottle 31. The first pump 81 may be a liquid pump. The test tube 21 is also connected to the outside environment via a ventilation tube 71, and an air pump 85 may be provided on the ventilation tube 71. The first pump 81, the air pump 85 are each electrically connected (i.e., communicatively connected) to the control device 5, and are controllably operated, thereby controlling the reagent in the test tube 21, and thus completing the experiment.

Fig. 2 shows a schematic view of a second embodiment of a pathological tissue producing device according to the invention and variants thereof. On the basis of the above embodiment, a second reagent cartridge 4 is also provided in the pathological tissue producing apparatus, in which another reagent bottle (i.e., a second reagent bottle 41) is placed. The second reagent bottle 41 is connected to the test tube 21 in the tissue magazine 2 by a second conduit 62. A second pump 82 is provided on the second conduit 62 for transporting the reagent into or out of the test tube 21 in the tissue magazine 2. The same reagent can be placed in the first reagent bottle 31 and the second reagent bottle 41. The two reagent bottles (i.e., the first reagent bottle 31 and the second reagent bottle 41) can also be used for placing different reagents so as to meet different experimental environment requirements, and thus, the reagents in the reagent chambers do not need to be adjusted in the experimental process.

In a variant of the second embodiment, a first on-off valve 91, a second on-off valve 92, a fifth on-off valve 95 are provided on the first conduit 61, the second conduit 62 and/or the airway tube 71, which are used to control the liquid transported in each conduit and thus the different reagents into the tissue cartridge 2 at different stages of the experiment. The first pump 81, the second pump 82, and the air pump 85 control inflow and outflow of each reagent. A fluid level detector (not shown) is provided on the airway tube 71 and/or fluid level detectors (not shown) are provided on the first and second tubes 61, 62 which are communicatively connected to the control device 5 for detecting the fluid level in the test tube 21.

When the reagent is supplied to the test tube 21, the air pump 85 is in an exhaust state or an idle state, and the first pump 81 or the second pump 82 is adjusted to be in a liquid supply state. When the liquid level detector (liquid sensor) detects the liquid, the air pump 85 and either the first pump 81 or the second pump 82 are stopped. When the reagent needs to be replaced, the air pump 85 is turned on to a state of inputting the gas, the first pump 81 or the second pump 82 is reversely rotated or in an empty state, and the air pump 85 inputs the air into the test tube 21 through the ventilation duct 71, thereby discharging the liquid out of the test tube 21 (at this time, the test tube 21 is sealed by a cap not shown). When the liquid level detector of the first conduit 61 or the second conduit 62 detects that the liquid level has fallen below the predetermined position, the air pump 85 and the first pump 81 or the second pump 82 are stopped. Then, the steps at the time of reagent input are repeated, and the replacement reagent is input into the test tube 21.

Fig. 3 shows a schematic view of another variant of a second embodiment of a pathological tissue making apparatus according to the invention. On the basis of the above embodiment, the test tube ends of the first and second tubes 61, 62 may be joined together in a common one tube to be connected to the test tube 21. At this time, the reagents in the first and second reagent bottles 31 and 41 may be selected to enter the test tube 21 by the first and second on-off valves 91 and 92 and/or the first and second pumps 81 and 82.

In a further variant of the second embodiment shown in fig. 4, the pathological tissue producing device also has a first return conduit 63 and a second return conduit 64 connected between the test tube 21 and the first and second reagent bottles 31, 41, respectively. The first return conduit 63 and the second return conduit 64 are used for returning the reagent in the test tube 21 to the corresponding first reagent bottle 31 and second reagent bottle 41, thereby realizing the recycling of the reagent. The first return conduit 63 and the second return conduit 64 may have on-off valves and/or pumps. Similarly, in the first embodiment shown in fig. 1, a first return conduit 63 may also be provided between the test tube 21 and the first reagent bottle 31.

Meanwhile, the length of the first return conduit 63 extending into the test tube 21 can be adjusted according to actual use requirements; the length of the first return conduit 63 extending into the respective first and second reagent bottles 31, 41 may also be adjusted according to actual use requirements. For example, when the reagent needs to circulate in both directions through the test tube 21, the first return conduit 63 extends into the first reagent bottle 31 and the second reagent bottle 41, and the first return conduit 63 extends into the bottom of the first reagent bottle 31 and the second reagent bottle 41, and the reagent can enter the test tube 21 from the first conduit 61 or enter the test tube 21 from the first return conduit 63 in combination with the use of the first pump 81. When the reagent flows through the test tube 21 and needs to circulate unidirectionally, the length of the first return conduit 63 extending into the first reagent bottle 31 and the second reagent bottle 41 is short, and the reagent liquid level in the first reagent bottle 31 and the second reagent bottle 41 does not exceed the bottom of the first return conduit 63. At this time, when the first pump 81 is operated in the forward direction, the reagent continuously enters the test tube 21 from the first reagent bottle 31 and the second reagent bottle 41 through the first conduit 61, and returns to the corresponding first reagent bottle 31 and second reagent bottle 41 from the first return conduit 63, thereby realizing the recycling of the reagent. When the first pump 81 is operated in the reverse direction, the first return conduit 63 sucks air from the first and second reagent bottles 31, 41, and the reagent returns to the corresponding first and second reagent bottles 31, 41 through the first conduit 61.

In a further variant of the second embodiment shown in fig. 5, the pathology tissue preparation apparatus further has a first reagent temporary storage bottle 65 and a second reagent temporary storage bottle 66. The first reagent temporary storage bottle 65 and the second reagent temporary storage bottle 66 are connected to the test tube 21 via a first top pipe and a second top pipe, respectively, for temporarily storing the reagent flowing out of the test tube 21 therein or pushing the reagent temporarily stored therein into the test tube 21, thereby realizing the recycling of the reagent. For example, the first reagent buffer bottle 65 is used to buffer the reagent originally from the first reagent bottle 31, and the second reagent buffer bottle 66 is used to buffer the reagent originally from the second reagent bottle 41. One of the first reagent temporary storage bottle 65 and the second reagent temporary storage bottle 66 may be located on the ventilation catheter 71. Similarly, in the first embodiment shown in fig. 1, a first reagent buffer bottle 65 may be provided which is connected to the test tube 21 via a first top conduit. The reagent temporary storage bottles are positioned in the tissue bins 2 (shown in fig. 5) and can also be positioned in corresponding reagent bins. A liquid level detector (not shown) is provided in or on the conduit for venting corresponding to the first reagent temporary storage bottle, the second reagent temporary storage bottle, which is communicatively connected to the control means 5 for detecting the liquid level of the first reagent temporary storage bottle, the second reagent temporary storage bottle or the conduit for venting.

Fig. 6 shows a schematic view of a third embodiment of a pathological tissue producing device according to the invention. On the basis of the above-described embodiments, a plurality of reagent bottles (two first reagent bottles 31 or two second reagent bottles 41 are shown in fig. 6) are contained in the first reagent cartridge 3 and/or the second reagent cartridge 4. It should be noted that the reagent bottle placement layout shown in the figures is merely illustrative, and that a plurality of reagent bottles may be placed in other ways. For example, a plurality of reagent bottles may be arranged in two or more layers above each other, and one or more reagent bottles may be placed on each layer. The plurality of reagent bottles contain different reagents or the same reagent, and each reagent bottle is independently connected to the first conduit 61 or the second conduit 62 via a corresponding plurality of reagent-side branch conduits to the first switch valve 91, the second switch valve 92, the third switch valve 93, and the fourth switch valve 94. Each reagent bottle corresponds to an independent first, second, third, and fourth switching valve 91, 92, 93, 94, and the first, second, third, and fourth switching valves 91, 92, 93, 94 are electrically connected (i.e., communicably connected) to the control device. The combination of the two pumps (i.e., the first pump 81 and the second pump 82) can realize that a plurality of reagent bottles in a plurality of reagent chambers (i.e., the first reagent chamber 3 and the second reagent chamber 4) are respectively connected with the test tubes 21 in the tissue chamber 2, and the control device 5 can realize that the reagent in any reagent bottle can enter the test tubes 21 from the reagent bottle at any time or can be discharged from the test tubes 21 to the reagent bottle at any time. As shown in fig. 6, for example, two first reagent bottles 31 in the first reagent vessel 3 share a first conduit 61 downstream of the first and third switching valves 91 and 93, so that in a preferred example, two reagent bottles in the same reagent vessel are used to hold the same reagent in order to avoid mutual contamination of the reagents. In the embodiment shown in fig. 6, the two pumps (i.e., the first pump 81, the second pump 82) are located on a common section of the first conduit 61, the second conduit 62, respectively. In a variant, instead of providing a pump on the above-mentioned common section, a pump and/or a switching valve may be provided on each reagent-side branch conduit.

The two on-off valves (e.g., the first on-off valve 91, the third on-off valve 93) located on the same conduit may be replaced by a controlled three-way valve that is operable to selectively communicate the first conduit 61 with either the first reagent bottle 31 or the second reagent bottle 41 to perform the function of the two on-off valves in the figures. Similarly, when there are multiple on-off valves, they can be replaced by one controlled multi-way valve. The multi-way valve is located at the junction of the plurality of reagent-side branch conduits and the common first conduit 61, so that the first conduit 61 can be selectively communicated with the corresponding reagent bottle by controlling the multi-way valve. Optionally, the three-way valve or multi-way valve is used for gating the reagent flow path, the material or coating of which is capable of withstanding the corrosion of chemical agents including, but not limited to, various concentrations of alcohol, benzene solution, paraffin (high temperature melt state); optionally, the three-way valve or the multi-way valve can withstand repeated changes in temperature, with a temperature range of at least 0 ℃ to 70 ℃.

The pathological tissue making device is further provided with a housing (not shown) in which the tissue magazine 2, the first reagent magazine 3, the second reagent magazine 4 are arranged, the housing being for protecting the structures therein.

Fig. 7 shows a schematic view of a variant of a third embodiment of a pathological tissue producing device according to the invention. On the basis of the above described embodiments, the different reagent bottles are connected to the test tube via completely separate conduits, i.e. without a common conduit section, with respective separate liquid pumps on the completely separate conduits for separately feeding the reagents into the test tube.

In another variation of the third embodiment shown in fig. 8, the plurality of first conduits connected to the plurality of first reagent bottles 31 in the first reagent vessel 3 may be collected via the first flow divider 67 into one conduit which is then connected to the test tube 21, and the plurality of second conduits connected to the plurality of second reagent bottles 41 in the second reagent vessel 4 may be collected via the second flow divider 68 into one conduit which is then connected to the test tube 21. In a further variant, the plurality of first conduits and the plurality of second conduits may be collected into one conduit via the same shunt and then connected to the test tube 21. The diverter, the first diverter and the second diverter are all used for liquid diversion and can be a liquid diversion structure combined with the electric control switch valve. The function of these diverters is to control the opening and closing of the multiple passages of the diverter by electrically controlling the multiple on-off valves for selectively controlling which one or more of the multiple first conduits can be circulated. These splitters may also be replaced by liquid tee or liquid multipass. Optionally, the flow divider, first flow divider, second flow divider are used for gating the reagent flow path, the material or coating of which is capable of withstanding corrosion by chemical agents including, but not limited to, various concentrations of alcohol, benzene solution, paraffin (high temperature melt state); optionally, the splitter, the first splitter, the second splitter are capable of withstanding repeated changes in temperature, the temperature range being at least 0 ℃ to 70 ℃.

Fig. 9 shows a schematic view of a fourth embodiment of a pathological tissue producing device according to the invention, wherein the control means are not shown. On the basis of the above embodiment, a plurality of test tubes (three test tubes 21 are shown in fig. 9) are contained in one tissue magazine 2. In this embodiment, multiple test tubes are used, multiple test environments may be performed in parallel, and multiple tests may be performed synchronously or asynchronously. Specifically, there are a plurality of test tube end branch ducts converging to the common first duct 61, a plurality of test tube end branch ducts converging to the common second duct 62, and a plurality of ventilation ducts 71, corresponding to the number of test tubes. The first reagent cartridge 3 is connected to the plurality of test tubes in the tissue cartridge 2 via the first pump 81, the first conduit 61, and the plurality of test tube end branch conduits, respectively, and the second reagent cartridge 4 is connected to the plurality of test tubes in the tissue cartridge 2 via the second pump 82, the second conduit 62, and the plurality of test tube end branch conduits, respectively. The plurality of ventilation ducts 71 are connected to an air pump. Some or all of the plurality of tube-end branch ducts and/or the plurality of ventilation ducts 71 are provided with on-off valves. The pump, conduit, on-off valve structures provided in the same tissue cartridge 2 may be controlled individually or together. If the experimental steps are identical, multiple sets of experiments can be controlled in the same manner. If the experimental steps are different, then multiple sets of experiments are controlled in different ways to perform different experiments in an environment that the same tissue cartridge 2 can provide. By using the same tissue warehouse 2 and the same group of temperature regulation operation steps, a plurality of groups of experiments can be parallel in the same tissue warehouse, thereby improving the efficiency of pathological experiments and avoiding the new tissue warehouse and the corresponding temperature rise and drop steps required by the experiments.

Fig. 10 shows a schematic view of a fifth embodiment of a pathological tissue producing device according to the invention, wherein the control means and the reagent cartridge are not shown. On the basis of the above embodiment, a plurality of tissue bins (three tissue bins 2 are shown in fig. 10) are provided, and each tissue bin can be a test tube experimental environment or a plurality of experimental environments. It should be noted that the tube placement layout shown in fig. 10 is merely illustrative, and that multiple tubes may be placed in other ways. For example, the plurality of test tubes may be arranged in two or more layers above each other, and each layer may house one or more test tubes. Specifically, there are tissue bins 2, each of which is connected to a reagent bin or the external environment by a corresponding conduit, ventilation conduit, on-off valve, pump, etc., which are not described here again. A temperature sensor (not shown) is provided in each tissue compartment, and the individual tissue compartments may have the same or different temperatures. Each tissue cartridge has an independent conduit, an airway tube, a first pump and a second pump so that the automatic dehydration, transparency and/or waxing treatments can be performed independently and without interfering with each other. Therefore, a plurality of tissue bins can be parallelly and simultaneously tested, the flexibility of the test is improved, and the test efficiency is improved.

Advantageously, in various embodiments, the flow rate of the first pump and/or the second pump of each tissue cartridge is adjustable and the flow direction is adjustable. The flow rate of the liquid in the conduit is controlled by controlling the flow rate of the first pump and/or the second pump, thereby controlling the experimental environmental parameters.

Advantageously, in various embodiments, a temperature control structure is further provided, as well as a temperature detection structure. Specifically, a temperature sensor is arranged in the tissue cabin, and the temperature sensor is electrically connected with the control device, so that the control device monitors the temperature in the control cabin.

Advantageously, the tissue compartment is provided with a cooling device which cools the tissue compartment. Additionally or alternatively, the tissue cartridge is provided with a heating device that warms the tissue cartridge. The heating device may be one or more of a heating wire and a heating bulb. The refrigeration device can be one or more of a semiconductor refrigeration sheet, a radiator fan, a heat exchanger and a liquid cooling device. In addition, the tissue warehouse can be further provided with a temperature equalizing device, which can be a temperature equalizing fan (for air temperature equalizing) and a temperature equalizing turbine (for temperature regulating liquid temperature equalizing in the liquid bath warehouse). The temperature environment in the tissue cabin can be changed through the refrigerating equipment and/or the heating equipment and/or the temperature equalizing equipment, so that the requirements of different experimental contents and different experimental steps on the environmental temperature are met.

Fig. 11 shows a schematic view of a sixth embodiment of a pathological tissue producing device according to the invention, wherein the control means are not shown. As shown in fig. 11, the pathological tissue producing apparatus further comprises a first cartridge (in particular, a high Wen Shuicang 6), a second cartridge (in particular, a low-temperature water cartridge 7), and a first recovery cartridge 8, a second recovery cartridge 9. The tissue magazine 2 shown in fig. 11 takes the form of a liquid bath magazine (in particular, a water bath magazine) as a whole, i.e. a liquid bath (in particular, a water bath) is formed in the interior space of the tissue magazine, in which the test tube 21 is partially or completely immersed. At this time, the test tube 21 is sealed so that the liquid in the liquid bath does not enter the test tube 21. Alternatively, the liquid bath cartridge may be formed in an interlayer of the housing of the tissue cartridge 2, i.e. the housing of the tissue cartridge 2 may be formed as an inner and an outer layer spaced apart and a liquid bath is formed between the inner and outer layers, the test tube 21 not being immersed in the liquid bath. The liquid bath bin is connected with the high-temperature water bin 6 and the low-temperature water bin 7 through a switch valve and a conduit. The connecting interface for connecting the high-temperature water bin 6 and the low-temperature water bin 7 is positioned at the bottom of the liquid bath bin. When the high-temperature environment is needed in the liquid bath cabin, the switch valve connected with the high-temperature water cabin 6 is opened, high-temperature liquid in the high-temperature water cabin 6 enters the liquid bath cabin through the guide pipe, and when the high-temperature liquid reaches a preset height, the switch valve connected with the high-temperature water cabin 6 is closed. At this point, the temperature control device in the liquid bath cabin regulates/maintains the temperature in the liquid bath cabin. When a low-temperature environment is needed in the liquid bath cabin, a switch valve connected with the first recovery cabin 8 is opened, and high temperature water flows into the first recovery cabin 8 through a conduit. The first recovery bin 8 maintains the temperature of the high-temperature liquid through the temperature control device, and the high-temperature liquid can be recovered to the high-temperature water bin 6 through the first water return pump 87 to realize the recycling of the liquid. When the high-temperature liquid in the liquid bath bin is emptied, the switch valve connected with the first recovery bin 8 is closed, and the switch valve connected with the low-temperature water bin 7 is opened, so that the low-temperature liquid flows into the liquid bath bin through the guide pipe, and the rapid cooling of the experimental environment in the liquid bath bin is realized. The temperature in the liquid bath bin can be maintained by a temperature control device in the liquid bath bin. When the low-temperature liquid in the liquid bath bin needs to be emptied, a switch valve connected with the liquid bath bin and the second recovery bin 9 is opened, and the low-temperature liquid in the liquid bath bin is discharged. The temperature control device in the second recovery tank 9 controls/maintains the temperature of the low-temperature liquid, and when the temperature of the low-temperature liquid satisfies the low-temperature liquid temperature requirement, the low-temperature liquid in the second recovery tank 9 is sent to the low-temperature water tank 7 by the second water return pump 88. In a simplified variant, the pathological tissue producing device may have only one recovery cartridge. In another more simplified variant, the pathological tissue producing device may not have a recovery cartridge, and the water of the liquid bath cartridge may be returned directly to the respective high-temperature or low-temperature water cartridge and returned therein to the high or low temperature by the temperature control means.

In one variant, the pathological tissue making device comprises only the high-temperature water bin 6, not the low-temperature water bin 7. When the temperature requirement of the experimental environment on the low-temperature environment is not harsh, the pathological tissue making equipment only comprises a high-temperature water bin 6, when the low-temperature environment is needed in the liquid bath bin, water is not added in the liquid bath bin, or water in the existing liquid bath bin is discharged into a recovery bin, and then the environment is cooled naturally or through other modes without liquid bath such as air cooling; optionally, a reagent (low-temperature liquid) is introduced into the test tube 21, and the test is performed by introducing the low-temperature liquid into the test tube, so that the temperature requirement in the test tube 21 is met. When the high-temperature environment is needed in the liquid bath cabin, the switch valve connected with the high-temperature water cabin 6 is opened, high-temperature water in the high-temperature water cabin 6 enters the liquid bath cabin through the guide pipe, and when the high-temperature water reaches a preset height, the switch valve connected with the high-temperature water cabin 6 is closed. When the experiment is finished, a switch valve for water outlet of the liquid bath cabin is opened, and high temperature water in the liquid bath cabin is emptied. The temperature control device of the recovery bin regulates the water in the recovery bin to the temperature of the high-temperature water bin 6, and the water temperature in the first recovery bin 8 reaches the temperature of the high-temperature water bin 6, and the first water return pump 87 is opened to return the water in the first recovery bin 8 to the high-temperature water bin 6 so as to realize the recycling of the high-temperature water; or the high-temperature water in the liquid bath bin is directly discharged back to the high Wen Shuicang, and the temperature is recovered in the high-temperature water bin.

Preferably, the first storage bin and the liquid bath bin are controlled by a pump to realize liquid circulation, and liquid flow type temperature control can also be realized, namely, liquid in the liquid bath bin continuously flows. During the experiment, when the first temperature is required to be maintained, the liquid at the first temperature in the first storage bin is continuously fed into the liquid bath bin, so that the liquid at the first temperature in the liquid bath bin flows out of the liquid bath bin, and the liquid bath bin is maintained at the first temperature, thereby minimizing the influence of heat transfer. Meanwhile, when the liquid bath bin needs to be maintained at the second temperature, the liquid at the second temperature in the second storage bin is continuously supplied into the liquid bath bin, so that the liquid at the second temperature in the liquid bath bin flows out of the liquid bath bin, the liquid bath bin is maintained at the second temperature, and the influence of heat transfer is minimized.

Preferably, the first storage bin and the recovery bin (or the first recovery bin) and the liquid bath bin are circulated by a pump; the second storage bin and the recovery bin (or the second recovery bin) and the liquid bath bin realize liquid circulation through a pump; the third storage bin and the recovery bin (or the third recovery bin) and the liquid bath bin realize liquid circulation through a pump, and the like.

In another variation, more structural and positional relationships may be defined in order to achieve adjustment of the height of water in the control liquid bath. For example, one or more liquid level detectors are arranged in the liquid bath bin, the liquid level detectors are arranged at different heights in the liquid bath bin, and the liquid level in the liquid bath bin is judged through the liquid level detectors.

The high-temperature water bin 6 and/or the low-temperature water bin 7 are arranged at a position higher than the tissue bin 2, and liquid in the high-temperature water bin 6 and/or the low-temperature water bin 7 flows into the tissue bin 2/the liquid bath bin by utilizing gravity. The position of the recovery bin is lower than that of the tissue bin, and the liquid in the liquid bath bin flows into the recovery bin by utilizing gravity. Or the positions of the first recovery bin 8 and the second recovery bin 9 are lower than the tissue bin 2, and the liquid in the liquid bath bin flows into the first recovery bin 8 and the second recovery bin 9 by utilizing gravity.

Alternatively, the high-temperature water bin 6 and/or the low-temperature water bin 7 are connected with the liquid bath bin through a water pump (not shown), and the recovery bin is connected with the liquid bath bin through a water pump (not shown). Alternatively, the first recovery tank 8 and the second recovery tank 9 are connected to the liquid bath tank by a water pump (not shown). And controlling the liquid in the liquid bath bin to enter and exit through a water pump.

On the basis of the modification, water or other temperature-regulating liquid can be used as the temperature-regulating liquid in the liquid bath cabin. The temperature regulating liquid should have a high specific heat capacity in order to achieve the effect of rapid regulation of the temperature in the liquid bath.

On the basis of the above embodiment or the modification thereof, the tissue treatment device further comprises an air pump which is connected with the tissue warehouse 2 through an air pipe and a switching valve, wherein the air pump has the functions of pressure increasing and pressure reducing, and the switching valve is electrically connected with the control device 5. The air pump and the air pipe can be matched with a switch valve/a water pump of a high-temperature water bin, a low-temperature water bin and a recovery bin (or a first recovery bin and a second recovery bin) to finish water injection and drainage in the liquid bath bin.

On the basis of the above-described embodiments or variations thereof, the pathological tissue producing apparatus comprises a plurality of reservoirs for supplying a temperature regulating liquid having a plurality of temperature ranges to the liquid bath reservoir, and/or comprises a plurality of recovery reservoirs. The recovery tanks are configured to receive the used temperature regulating fluid from the fluid bath tanks, restore the temperature of the received temperature regulating fluid to a corresponding one of the temperature ranges, and return the temperature restored temperature regulating fluid to a corresponding one of the storage tanks.

In various embodiments, the tissue magazine 2 also contains a soaking fan and/or turbine (not shown) inside. The soaking fan is used to quickly equilibrate the temperature inside the tissue compartment 2 when the tissue compartment 2 is temperature regulated in the absence of liquid. The turbine serves to rapidly equilibrate the temperature inside the tissue compartment 2 when liquid is present in the tissue compartment 2. The tissue warehouse 2 is provided with a refrigerating device which is used for accelerating the cooling speed when the tissue warehouse 2 needs to be cooled. The inside firing equipment that is provided with of tissue storehouse 2, firing equipment are used for accelerating the rate of heating up when tissue storehouse 2 needs to heat up to improve experimental efficiency.

In various embodiments, the viscosity of the reagents in the different reagent chambers varies as well as the temperature-dependent characteristics. In the experimental requirements, reagents with different temperatures and different consistencies need to flow in different experimental stages, so that more structures need to be defined. For example, the inner diameter of the first conduit 61 may be larger than the inner diameter of the second conduit 62, and the inner diameter of the second conduit 62 may be larger than the inner diameter of the first conduit 61. The outer walls of the first conduit, the ventilation conduit and the second conduit can be provided with heat insulation layers. The conduit with large inner diameter is used for flowing liquid with poor fluidity, so that the blockage of the conduit is avoided. The heat preservation is used for avoiding rapid change of liquid temperature in the guide pipe, so that reduction of liquid mobility in the guide pipe is avoided, and further, the influence of liquid temperature change on experimental results is avoided.

On the basis of the above embodiment, the first reagent chamber 3 may contain a plurality of reagent bottles, and each reagent bottle is connected to the first conduit 61 through an on-off valve and a conduit. The second reagent chamber 4 contains a plurality of reagent bottles, and each reagent bottle is connected with the second conduit 62 through a switch valve and a conduit. The first reagent vessel 3 and/or the second reagent vessel 4 may comprise temperature control means therein. The switch valve and the temperature control device are electrically connected with the control device. The ambient temperature in the two reagent chambers may be different. For example, the first reagent vessel 3 may be adjusted to an ambient temperature of 66 ℃, and the first reagent vessel may be allowed to hold a substance that solidifies at normal temperature and melts at high temperature, such as paraffin. The second reagent chamber 4 can be maintained at room temperature, and can hold substances which keep liquid at the room temperature, such as alcohol and benzene solutions with various concentrations. Multiple reagent bottles can be placed in the reagent bin, so that multiple solutions with the same environmental property can be placed in the same reagent bin at the same time. Therefore, in the experiment, the control device controls the switch valve to select the solution, so that the degree of automation and the experiment efficiency are further improved.

In various embodiments, the airway tube is provided with an air pump, and the air pump is connected with a plurality of test tubes in various modes. For example, the air pump is provided with one, a plurality of ventilation pipes are gathered together to be communicated with the air pump, and each ventilation pipe is provided with a switch valve. The switch valve is electrically connected with the control device and is controlled by the control device.

Alternatively, the air pump is provided with two, and a plurality of first air guide pipes are gathered together to be communicated with the first air pump, and a plurality of second air guide pipes are gathered together to be communicated with the second air pump, and each first air guide pipe and each second air guide pipe are provided with a switch valve. The switch valve is electrically connected with the control device and is controlled by the control device.

In various embodiments, the upper and lower ends of the test tube 21 in the tissue warehouse 2 are respectively provided with a tube port, and the upper end tube port and the lower end tube port are respectively connected with the ventilation catheter and the first catheter (or the first catheter and the second catheter) through catheters. Such connection forms a circuit for circulation of the liquid. The tissue sample is placed in a test tube. The experimental process is automatically controlled by controlling the opening and closing states of the switch valves, the pumps and the like through the control device, so that a more perfect automatic experimental process is realized.

In various embodiments, the plurality of on-off valves described above employ solenoid valves. The switch valves in the invention can select different types of electromagnetic valves according to the application, for example, the switch valve in the liquid conduit is selected as the electromagnetic valve for controlling the liquid; and the on-off valve on the air pipe selects the electromagnetic valve for controlling the air.

Preferably, the opening size (opening degree) of each switching valve is controllable, that is, the opening sizes of the gas switching valve and the liquid switching valve are multi-stage or steplessly adjustable, so that the flow rate of the gas or liquid can be controlled by the switching valves. The first pump (liquid pump), the second pump (liquid pump), the air pump and the on-off valve on the corresponding conduit/pipe of each pump are mutually matched, so that the liquid/gas flow rate can be adjusted, and the pressure of the liquid/gas can be adjusted. It is contemplated that the pump speed of each pump may also be adjustable so that the liquid/gas flow rate may be adjusted by controlling the pump speed. Additionally or alternatively, the liquid/gas flow rate is regulated by controlling the on-off valve in conjunction with the pump.

Fig. 12 shows a seventh embodiment of a pathological tissue making apparatus according to the invention, wherein only the tissue and reagent cartridges are shown. Based on the above embodiments, in order to increase the number of experimental environments, some or all of the plurality of test tubes in the tissue magazine may be connected in series with a catheter. In this way, one experimental environment can be changed to multiple experimental environments in series, reducing the number of pumps and conduits between the reagent cartridge and the tissue cartridge.

The first and second conduits can have a variety of connection means with the plurality of reagent bottles in the reagent cartridge. For example, multiple reagent bottles may be connected to a tissue cartridge using one reagent input pathway. In this embodiment, a plurality of tissue bins 2 are provided, each of which has a plurality of sets of serially connected test tubes disposed therein. The corresponding tube-end branch conduits of the bottommost tube (e.g., the lower three tubes 21 in fig. 12) of the plurality of sets of serially connected tubes in each tissue magazine are pooled into a common first conduit 61. The first conduit 61 is connected to the plurality of first reagent bottles 31 in the first reagent chamber 3 via the first pump 81, the plurality of first reagent bottles 31 are connected to the first conduit 61 via the plurality of reagent-side branch conduits, respectively, and a control switch valve is provided in each of the reagent-side branch conduits. The corresponding tube-end branch conduits of the bottommost tube (e.g., the lower three tubes 21 in fig. 12) of the plurality of sets of serially connected tubes in each tissue magazine are pooled into a common second conduit 62. The second conduit 62 is connected to a plurality of second reagent bottles 41 in the second reagent chamber 4 via a second pump 82, the plurality of second reagent bottles 41 are connected to the second conduit 62 via a plurality of reagent-side branch conduits, respectively, and a control switch valve is provided in each of the reagent-side branch conduits. The connection of the plurality of reagent-side branch catheters to the first catheter 61, the second catheter 62 and/or the connection of the plurality of test-tube-side branch catheters to the first catheter 61, the second catheter 62 may be achieved by a multi-lumen tube or a shunt. The corresponding airway branch conduits of the topmost test tube (e.g., the upper three test tubes 21 in fig. 12) of the plurality of series of test tubes in each tissue magazine are pooled into a common airway conduit 71, the airway conduit 71 being connected to an air pump 85. In this example, the number of reagent bottles is independent of the number of pumps, and there are only two pumps, and the types and the number of the reagent bottles are adjusted according to experimental requirements.

In one variation, each reagent bottle corresponds to a set of reagent input pathways. In this variant, a plurality of tissue bins 2 are provided, each of which has a plurality of sets of test tubes in series. Corresponding to the plurality of first reagent bottles 31 in the first reagent vessel 3, there are a corresponding (in particular, identical) plurality of first conduits 61 and a plurality of first pumps 81. Similarly, there are a corresponding (in particular, identical) number of second conduits 62 and a plurality of second pumps 82 corresponding to the plurality of second reagent bottles 41 in the second reagent cartridge 4. The bottommost tube of the plurality of sets of serially connected tubes in each tissue magazine contains a corresponding (in particular, identical) number of tube-end branch tubes, converging to a corresponding first tube and a corresponding second tube, respectively. For example, if m reagent bottles are contained in the first reagent cartridge 3 and n reagent bottles are contained in the second reagent cartridge 4, m first pipes 61 (and m first pumps 81) and n second pipes 62 (and n second pumps 82) are provided correspondingly. This ensures that one reagent bottle is connected to multiple sets of serially connected test tubes by a single conduit. The number of conduits and pumps is related to the number of reagent bottles, in particular the same as the number of reagent bottles. The types and the quantity of the reagent bottles are adjusted according to experimental requirements.

In another variant, the case of a plurality of reagent bottles corresponding to one reagent input channel occurs simultaneously with the case of a single reagent bottle corresponding to one reagent input channel, i.e. the number of groups of test tubes in series does not correspond to the number of pumps. The quantity of reagent bottles is irrelevant with the quantity of pumps, the quantity of pumps is relevant with the quantity of reagent bins, under the condition of two reagent bins, the pumps are only two, the reagent is adjusted to enter and exit through the cooperation of the pumps and the switch valve, and the types and the quantity of the reagent bottles are adjusted according to experimental requirements. It is conceivable that in the case where a plurality of tissue bins are provided, the number of pumps is 2 times the number of tissue bins (in the case of two reagent bins).

In various embodiments, the interconnecting conduits of the present invention may be provided as integrally formed branch conduits. Alternatively, these conduits are separately formed and then joined at assembly to form a sealed conduit. Further, in the conduit used in the above embodiment, a heat-insulating conduit is fully or partially used, or a heat-insulating layer is provided outside the conduit, for insulating the high-temperature liquid in the transmission and/or insulating the low-temperature liquid in the transmission.

In various embodiments, the first conduit (or the first conduit and the second conduit) and the airway tube in the tissue cartridge may be connected and arranged in various ways. Specifically, a plurality of first conduits (or first and second conduits) in the tissue compartment are distributed at the bottom of the tissue compartment and a plurality of exhaust conduits are distributed on the side wall of the tissue compartment. The plurality of first conduits (or first conduits and second conduits) and the plurality of ventilation conduits are arranged in a regular pattern, e.g., equidistantly, around the side wall of the tissue cartridge, etc.

Further, a plurality of first conduits (or, first and second conduits) can pass through the tissue cartridge floor. Alternatively, the top end of the first conduit (or the first conduit and the second conduit) is flush with the bottom end of the tissue cartridge, or the bottom end of the first conduit (or the first conduit and the second conduit) is flush with the bottom end of the tissue cartridge. The reagent ends of the first conduit (or the first conduit and the second conduit) may be provided with a one-to-many conduit splitter, with multiple interface sides of the conduit splitter being connected to each reagent cartridge by the conduits. Similarly, the end of the first catheter (or the first catheter and the second catheter) may be provided with a multi-split catheter shunt, with multiple interface sides of the catheter shunt being connected to each of the test tubes by the catheter. Part of the outer wall of the conduit or all of the outer wall of the conduit is wrapped with an insulating layer. A plurality of ventilation catheters may pass through the tissue cartridge side wall. Alternatively, the top end of the airway tube is flush with the tissue cartridge side wall or the bottom end of the airway tube is flush with the tissue cartridge side wall. The top ends of the plurality of ventilation ducts are connected with an air pump. The air pump is a bidirectional air pump, and can output air and also can input air. The air pump is connected with a conduit communicated with the external environment.

Fig. 13 shows a schematic view of an eighth embodiment of a pathological tissue producing device according to the present invention. The pathological tissue making apparatus, in particular an incubator, comprises a tissue compartment 2, a first reagent compartment 3 and a control device 5. A test tube 21 is placed in the tissue magazine 2, and an experimental specimen 10 is placed in the test tube 21. The test tube may be a disposable replaceable consumable. The actual product shape of the tube may be similar to drip chambers used in intravenous sets. A first reagent bottle 31 is placed in the first reagent vessel 3, and the first reagent bottle 31 is connected to the test tube 21 in the tissue vessel 2 via a first conduit 61. The first conduit 61 is provided with a first pump 81, and the liquid (reagent) in the first reagent bottle 31 is transferred to the test tube 21 by the first pump 81. The first pump 81 may return the reagent in the test tube 21 to the first reagent bottle 31. The first pump 81 may be a liquid pump. The test tube 21 is also connected to the first reagent bottle 31 by a first return conduit 63. The first pump 81 is electrically connected (i.e., communicatively connected) to the control device 5 and controllably operated, thereby controlling the reagent in the test tube 21, enabling recycling of the reagent, and thus completing the experiment.

Further, the first reagent bottle 31 adopts a sealing structure, the first conduit 61 and the first return conduit 63 extend into the first reagent bottle 31 through two conduit holes at the top of the first reagent bottle 31, and the test tube 21 and the first reagent bottle 31 form a sealed experimental environment through the first conduit 61 and the first return conduit 63.

In a further variant, a plurality of first reagent bottles 31 are provided in the first reagent vessel 3, wherein at least one first reagent bottle 31 adopts a sealing structure; the pathological tissue making device is provided with a plurality of first guide pipes 61 which are respectively connected to the plurality of first reagent bottles 31 from test tubes in the tissue warehouse 2, the plurality of first guide pipes 61 are converged to the test tubes through a shunt, and the shunt can independently control the on-off of any first guide pipe 61. When the flow divider controls a certain path of first conduit 61 to be opened, if the corresponding first reagent bottle 31 adopts a sealing structure, the first reagent bottle 31 and the test tube 21 form a sealed experimental environment through the corresponding first conduit 61 and the corresponding first return conduit 63; when the flow divider controls a certain path of the first conduit to be opened, if the corresponding first reagent bottle 31 does not adopt a sealing structure, the first reagent bottle 31 is communicated with air, and the first reagent bottle 31 and the test tube 21 form an unsealed experimental environment through the corresponding first conduit 61 and the corresponding first return conduit 63.

The operation of the pathological tissue producing apparatus according to the present invention in experiments (especially pathological tissue production) will be explained below. The processing environments and processing time required by different tissue specimens are different, each tissue bin independently adopts a corresponding optimal manufacturing scheme, and the control is realized through a control device. Each tissue warehouse operates independently, and the temperatures do not influence each other. According to the number and variety of the specimens per day, a corresponding number of tissue bins are started. When the number of specimens is small, no reagent is wasted; when the tissue is needed urgently, the rest tissue warehouse can be opened at any time.

In the experimental process, after the dehydration and transparency are finished, the water in the stock solution bath bin is discharged into the recovery bin, and the water in the water bin (high-temperature water bin) at 66 ℃ is immediately injected into the liquid bath bin. Meanwhile, the target temperature of the tissue warehouse is adjusted to 66 ℃, the temperature difference of the tissue warehouse temperature of 29 ℃ is quickly increased, the temperature is jumped from 37 ℃ to 62-66 ℃, timely wax dipping is ensured, meanwhile, the temperature is constant and accurate, and the risk of overheating is avoided. After the waxing is finished, the heating of the tissue warehouse is stopped, the water in the liquid bath warehouse is immediately discharged back to the recovery warehouse, and meanwhile, the room temperature water (low-temperature liquid) is injected into the liquid bath warehouse, so that the temperature of the tissue warehouse is quickly reduced to the room temperature, excessive waxing is avoided, and the quality and safety of flaking are ensured.

Each tissue cabin independently operates, and a group of test tubes, corresponding structures such as pumps, first catheters (or the first catheters and the second catheters), ventilation catheters and the like can be arranged in the tissue cabin to carry out pathological experiments. A plurality of test tubes and corresponding structures such as pumps, first catheters (or first and second catheters), airway tubes, and the like may also be disposed within the tissue tract. Parallel pathological specimen operations can be performed by using the same tissue bin. The tissue warehouse is used for adjusting the environment temperature in the tissue warehouse by injecting high-temperature water and low-temperature water into the liquid bath interlayer of the outer wall of the tissue warehouse through the high Wen Shuicang low-temperature water warehouse, and soaking equipment in the tissue warehouse is used for accelerating the temperature equalization in the tissue warehouse. The test tube is put into the tissue cabin, and the tissue cabin is connected with the first reagent cabin and the second reagent cabin through the first catheter and the second catheter. The paraffin is placed in the first reagent storehouse, and thus needs through maintaining certain temperature guarantee flowable liquid, and each first pipe (or first pipe and second pipe) of connecting first reagent storehouse, ventilation pipe all set up the heat preservation, and connect each first pipe of first reagent storehouse, ventilation pipe's pipe wall internal diameter is greater than each second pipe of connecting the second reagent storehouse, in ventilation pipe tissue storehouse for maintain the flowability of first reagent storehouse interior liquid, avoid the jam. Each tissue compartment is provided with a temperature regulating device for regulating the temperature in the tissue compartment.

The technical features of the above-described embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above-described embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description. In other words, the non-conflicting elements of the above-described embodiments may be substituted or supplemented with each other to form a new embodiment.

The above embodiments represent only a few embodiments of the present invention, which are described in more detail and are not to be construed as limiting the scope of the present invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention.

Claims

1. A pathological tissue producing apparatus, characterized in that the pathological tissue producing apparatus comprises:

a tissue magazine (2) in which one or more tube sets are provided, each tube set comprising a plurality of tubes (21) connected in series, an experimental specimen (10) being placed in said tubes (21) and being processed in said tubes (21);

A first reagent chamber (3) in which a first reagent bottle (31) is provided;

-a first conduit (61) connecting the first reagent bottle (31) to the test tube (21) in the tissue cartridge (2); a first pump (81) provided on the first conduit (61) for delivering the reagent in the first reagent bottle (31) into the test tube (21);

an airway tube (71) having one end connected to the test tube (21) and the other end connected to the outside air of the test tube; and

-a control device (5) electrically connected to said first pump (81) so as to cause a controlled operation of said first pump (81).

2. Pathological tissue producing device according to claim 1, characterized in that said first catheter (61) has at one end of said tissue magazine (2) a respective one or more first tube-end branch catheters connected to said one or more tube sets, respectively.

3. The pathological tissue producing apparatus according to claim 1, further comprising a first reagent temporary storage bottle (65), the first reagent temporary storage bottle (65) being connected to the one or more test tube groups by a first top conduit, wherein the first top conduit has at an end remote from the first reagent temporary storage bottle (65) a respective one or more first top branch conduits connected to the one or more test tube groups, respectively.

4. Pathological tissue producing device according to claim 1, characterized in that the ventilation catheter (71) has a respective one or more ventilation branch catheters connected to the one or more test tube sets, respectively.

5. A pathological tissue producing device according to claim 1, characterized in that it has a respective one or more ventilation ducts (71) connected to the one or more groups of tubes, respectively.

6. Pathological tissue producing device according to claim 1, characterized in that a plurality of first reagent bottles (31) are provided in the first reagent cartridge (3), and that the first conduit (61) has at one end of the first reagent cartridge (3) a plurality of first reagent-end branch conduits connected to the plurality of first reagent bottles (31), respectively.

7. A pathological tissue producing device according to claim 1, characterized in that a plurality of first reagent bottles (31) are provided in the first reagent cartridge (3), and that the pathological tissue producing device has a plurality of first conduits (61) connected from the test tubes in the tissue cartridge (2) to the plurality of first reagent bottles (31), respectively.

8. The pathological tissue producing device according to claim 7, wherein the plurality of first conduits (61) are funneled to the test tube via a shunt.

9. Pathological tissue-making device according to claim 4, characterized in that the first catheter (61) has at one end of the tissue magazine (2) a respective one or more first tube-end branch catheters connected to the one or more tube groups, respectively, wherein one or more of the plurality of first tube-end branch catheters, the plurality of ventilation branch catheters is provided with a switching valve and/or one or more of the plurality of ventilation branch catheters is provided with an air pump.

10. Pathological tissue producing device according to claim 2, characterized in that said plurality of first tube-end branch ducts are connected to a common first duct (61) via a multiport valve.

11. The pathological tissue-producing device according to claim 5, wherein the first catheter (61) has at one end of the tissue magazine (2) a respective one or more first tube-end branch catheters connected to the one or more tube sets, respectively, wherein one or more of the plurality of first tube-end branch catheters, the plurality of ventilation catheters are provided with an on-off valve.

12. The pathological tissue producing apparatus according to claim 6, wherein one or more of the plurality of first reagent-end branch ducts are provided with an on-off valve.

13. A pathological tissue producing device according to claim 1, further comprising a first return conduit (63), one end of the first return conduit (63) being connected to the test tube and the other end being connected to the first reagent bottle (31).

14. A pathological tissue producing apparatus according to claim 1, further comprising:

a second reagent chamber (4) in which a second reagent bottle (41) is provided;

-a second conduit (62) connecting the second reagent bottle (41) to the test tube (21) in the tissue cartridge (2); a second pump (82) arranged on the second conduit (62) for delivering the reagent in the second reagent bottle (41) into the test tube (21).

15. Pathological tissue producing device according to claim 14, wherein one or more tube sets are provided in the tissue magazine (2), each tube set comprising one tube or a plurality of tubes connected in series, wherein the first (61) and the second (62) tube are gathered at one end of the tissue magazine (2) into one common tube, which is connected to the respective one or more tube sets via one or more branch tubes.

16. Pathological tissue producing device according to claim 14, characterized in that one or more test tube groups are provided in the tissue magazine (2), each test tube group comprising one test tube or a plurality of test tubes connected in series; at least one of the one or more tube sets receives reagent via only one inlet located at the bottom of the tube and/or at least one of the one or more tube sets discharges reagent via only one outlet located at the top of the tube.

17. A pathological tissue producing apparatus according to claim 14, wherein one or more tube sets are provided in the tissue magazine (2), each tube set comprising one tube or a plurality of tubes connected in series, the pathological tissue producing apparatus further comprising a second reagent temporary storage bottle (66), the second reagent temporary storage bottle (66) being connected to the one or more tube sets by a second top conduit, wherein the second top conduit has at an end remote from the second reagent temporary storage bottle (66) a respective one or more second top branch conduits connected to the one or more tube sets, respectively.

18. Pathological tissue producing device according to claim 14, characterized in that on-off valves are provided on the first (61) and/or second (62) and/or ventilation (71) conduits.

19. Pathological tissue producing device according to claim 14, characterized in that a level detector is provided on the ventilation catheter (71) and/or the first catheter (61) and/or the second catheter (62).

20. Pathological tissue producing device according to claim 14, wherein one or more tube groups are provided in the tissue magazine (2), each tube group comprising one tube or a plurality of tubes connected in series, wherein the first conduit (61) has at one end of the tissue magazine (2) a respective one or more first tube-end branch conduits connected to the one or more tube groups, respectively, and the second conduit (62) has at one end of the tissue magazine (2) a respective one or more second tube-end branch conduits connected to the one or more tube groups, respectively.

21. Pathological tissue producing device according to claim 14, characterized in that a plurality of first reagent bottles (31) are provided in the first reagent cartridge (3), and that the first conduit (61) has at one end of the first reagent cartridge (3) a plurality of first reagent-end branch conduits respectively connected to the plurality of first reagent bottles (31), and/or,

The second reagent cartridge (4) is provided therein with a plurality of second reagent bottles (41), and the second conduit (62) has a plurality of second reagent-end branch conduits at one end of the second reagent cartridge (4) connected to the plurality of second reagent bottles (41), respectively.

22. Pathological tissue producing device according to claim 14, characterized in that a plurality of first reagent bottles (31) are provided in the first reagent cartridge (3) and that the pathological tissue producing device has a plurality of first conduits (61) connected to the plurality of first reagent bottles (31) respectively from test tubes in the tissue cartridge and,

the second reagent cartridge (4) is provided therein with a plurality of second reagent bottles (41), and the pathological tissue producing apparatus has a plurality of second conduits (62) respectively connected to the plurality of second reagent bottles (41) from test tubes in the tissue cartridge.

23. Pathological tissue producing device according to claim 22, characterized in that said plurality of first conduits (61) are collected via a first shunt (67) as one conduit connected to said test tube and/or,

the plurality of second conduits (62) are collected via a second splitter (68) into one conduit connected to the test tube.

24. The pathological tissue producing device according to claim 22, wherein the plurality of first catheters (61) and the plurality of second catheters (62) are collected via a first shunt (67) as one catheter connected to the test tube.

25. The pathological tissue producing apparatus according to claim 20, wherein one or more of the plurality of first tube-end branch ducts and the plurality of second tube-end branch ducts are provided with an on-off valve.

26. The pathological tissue producing apparatus according to claim 21, wherein one or more of the plurality of first reagent-end branch ducts and the plurality of second reagent-end branch ducts are provided with an on-off valve.

27. A pathological tissue producing device according to claim 14, further comprising a first return conduit (63), one end of said first return conduit (63) being connected to said test tube and the other end being connected to said first reagent bottle (31); and/or the number of the groups of groups,

the pathological tissue making device further comprises a second return conduit (64), one end of the second return conduit (64) is connected with the test tube, and the other end is connected with the second reagent bottle (41).

28. A pathological tissue producing apparatus according to any one of claims 1 to 27, further comprising temperature regulating means and a temperature sensor for detecting the internal temperature of the tissue cartridge (2),

the temperature adjusting device comprises soaking equipment arranged in the tissue warehouse (2), wherein the soaking equipment is used for enabling temperatures at different positions in the tissue warehouse (2) to be consistent; and/or the number of the groups of groups,

the temperature regulating device comprises refrigerating equipment arranged inside the tissue cabin (2), and the refrigerating equipment is used for cooling the tissue cabin (2); and/or the number of the groups of groups,

the temperature regulating device comprises heating equipment arranged inside the tissue cabin (2), and the heating equipment is used for heating the tissue cabin (2).

29. A pathological tissue producing apparatus according to any one of claims 1-27, further comprising a temperature regulating device comprising a liquid bath compartment arranged within the tissue compartment (2), and a temperature sensor for detecting the internal temperature of the tissue compartment (2), wherein the pathological tissue producing apparatus further comprises a first reservoir for supplying a temperature regulating liquid having a first temperature range to the liquid bath compartment.

30. The pathological tissue producing apparatus according to claim 29, wherein said temperature regulating means comprises soaking means arranged inside said tissue compartment (2) for bringing the temperatures at different positions inside said tissue compartment (2) to be uniform; and/or the number of the groups of groups,

31. A pathological tissue producing apparatus according to claim 29, wherein the used temperature regulating liquid in the liquid bath cartridge is returned directly to the first cartridge or via a recovery cartridge for receiving the used temperature regulating liquid from the liquid bath cartridge and restoring the temperature of the received temperature regulating liquid to the first temperature range.

32. A pathological tissue producing apparatus according to claim 29, further comprising a second reservoir for supplying a temperature regulating liquid having a second temperature range to the liquid bath reservoir, wherein the first reservoir and the second reservoir are in communication with the liquid bath reservoir via one inlet of the liquid bath reservoir or with the liquid bath reservoir via two inlets of the liquid bath reservoir, respectively.

33. A pathological tissue producing apparatus according to claim 32, wherein the used temperature regulating liquid in the liquid bath cartridge is returned to the respective first or second cartridge.

34. A pathological tissue producing apparatus according to claim 32, further comprising a recovery bin for receiving used temperature regulating fluid from the fluid bath bin, recovering the temperature of the received temperature regulating fluid to the first temperature range or the second temperature range, and returning the temperature recovered temperature regulating fluid to the respective first or second bin.

35. A pathological tissue producing apparatus according to claim 32, further comprising:

a first recovery bin (8) for receiving the used temperature-regulated liquid of the first temperature range from the liquid bath bin, recovering the temperature of the received temperature-regulated liquid to the first temperature range, and returning the temperature-recovered temperature-regulated liquid to the first storage bin; and

a second recovery bin (9) for receiving the used temperature-adjusting liquid of the second temperature range from the liquid bath bin, recovering the temperature of the received temperature-adjusting liquid to the second temperature range, and returning the temperature-recovered temperature-adjusting liquid to the second storage bin,

Wherein the first recovery bin (8) and the second recovery bin (9) are communicated to the liquid bath bin via one outlet of the liquid bath bin or are communicated to the liquid bath bin via two outlets of the liquid bath bin respectively.

36. A pathological tissue producing apparatus according to claim 35, wherein a switching valve and/or a pump is provided on one or more of the tubing between the first reservoir and the liquid bath reservoir, the tubing between the second reservoir and the liquid bath reservoir, the tubing between the liquid bath reservoir and the first recovery reservoir, the tubing between the liquid bath reservoir and the second recovery reservoir, the tubing between the first recovery reservoir and the first reservoir, and the tubing between the second recovery reservoir and the second reservoir.

37. The pathological tissue producing apparatus according to claim 35, wherein a cartridge temperature control module and a temperature sensor are provided in one or more of the first cartridge, the second cartridge, the first recovery cartridge and the second recovery cartridge, the cartridge temperature control module, the temperature sensor being electrically connected with the control device, wherein the cartridge temperature control module comprises a heating device and/or a cooling device.

38. Pathological tissue producing device according to claim 29, characterized in that the inner space of the tissue magazine (2) forms the liquid bath magazine and the test tube is immersed in the temperature regulating liquid of the liquid bath magazine.

39. A pathological tissue producing device according to claim 29, wherein the housing of the tissue cartridge (2) has spaced apart inner and outer layers, the space defined between the inner and outer layers forming the liquid bath cartridge.

40. A pathological tissue producing apparatus according to any one of claims 1-27, further comprising a temperature regulating device comprising a liquid bath compartment arranged within the tissue compartment (2), and a temperature sensor for detecting the internal temperature of the tissue compartment (2), wherein the pathological tissue producing apparatus further comprises a plurality of reservoirs for supplying the liquid bath compartment with a temperature regulating liquid having a plurality of temperature ranges.

41. A pathological tissue producing apparatus according to claim 40, further comprising one or more recovery bins for receiving used temperature regulating fluid from the fluid bath bin, recovering the temperature of the received temperature regulating fluid to one of the plurality of temperature ranges, and returning the temperature recovered temperature regulating fluid to a respective one of the plurality of bins.

42. A pathological tissue preparation device according to claim 1, characterized in that the pathological tissue preparation device comprises a plurality of tissue bins (2), a plurality of first catheters (61) and a plurality of ventilation catheters (71), wherein one or more tube sets are provided in each tissue bin (2), each tube set comprising a plurality of tubes (21) connected in series, and wherein the tubes in each tissue bin (2) are connected to the first reagent bottle (31) by means of the corresponding first catheter (61) and are in air communication with the outside of the tubes by means of a corresponding one of the plurality of ventilation catheters (71).

43. Pathological tissue preparation device according to claim 14, characterized in that it comprises a plurality of tissue bins (2), a plurality of first catheters (61), a plurality of second catheters (62) and a plurality of ventilation catheters (71), wherein one or more tube sets are provided in each tissue bin (2), each tube set comprising a plurality of tubes (21) connected in series, and wherein the tubes in each tissue bin (2) are connected to the first reagent bottle (31) by the corresponding first catheter (61), to the second reagent bottle (41) by the corresponding second catheter (62) and are in air communication with the outside of the tubes by the corresponding one of the plurality of ventilation catheters (71).

44. A pathological tissue producing apparatus according to claim 42 or 43, wherein the plurality of tissue bins (2) have a corresponding plurality of liquid bath bins, and the pathological tissue producing apparatus further comprises a plurality of reservoirs for supplying temperature regulating liquid having a plurality of temperature ranges to the plurality of liquid bath bins.

45. The pathological tissue producing apparatus of claim 44, further comprising one or more recovery bins for receiving used temperature regulating fluid from each of the plurality of fluid bath bins, restoring the temperature of the received temperature regulating fluid to one of the plurality of temperature ranges, and returning the temperature restored temperature regulating fluid to a corresponding one of the plurality of bins.

46. A pathological tissue producing apparatus according to any one of claims 14 to 27, wherein the first conduit has a larger tube inner diameter than the second conduit.

47. A pathological tissue producing device according to any one of claims 14 to 27, wherein the outer wall of one or more of the first conduit, the second conduit, the airway tube is provided with an insulating layer.

48. A pathological tissue producing device according to any one of claims 14 to 27, characterized in that temperature control means are provided in the first and/or second agent compartments (3, 4), which are electrically connected to the control means (5).

49. A pathological tissue producing apparatus according to claim 18, wherein the opening of the on-off valve is adjustable and/or the flow rate of the first pump and/or the second pump is adjustable.

50. A pathological tissue producing device according to any one of claims 1 to 27, wherein an air pump (85) is provided on the airway tube (71), the air pump (85) being electrically connected to the control means (5).

51. A pathological tissue producing device according to claim 1, further comprising a return conduit connected at one end to the test tube (21) and at the other end to the first reagent bottle (31).

52. A pathological tissue producing apparatus, characterized in that the pathological tissue producing apparatus comprises:

A first reagent chamber (3) in which a first reagent bottle (31) is provided;

a first return conduit (63) having one end connected to the test tube (21) and the other end connected to the first reagent bottle (31); and

53. A pathological tissue producing device according to claim 52, wherein said first reagent bottle (31) adopts a sealing structure, said first conduit (61), said first return conduit (63) extend into said first reagent bottle (31), and said test tube (21) and said first reagent bottle (31) form a sealed experimental environment via said first conduit (61), said first return conduit (63).

54. A pathological tissue producing apparatus according to claim 52, further comprising:

a second reagent chamber (4) in which a second reagent bottle (41) is provided;

-a second conduit (62) connecting the second reagent bottle (41) to the test tube (21) in the tissue cartridge (2); a second return conduit (64) having one end connected to the test tube (21) and the other end connected to the second reagent bottle (41);

a second pump (82) arranged on the second conduit (62) for delivering the reagent in the second reagent bottle (41) into the test tube (21).

55. The pathological tissue producing device according to claim 54, wherein said first reagent bottle (31) adopts a sealing structure, said first conduit (61), said first return conduit (63) extend into said first reagent bottle (31), said second pump (82) is not operated when said first pump (81) is operated, said test tube (21) and said first reagent bottle (31) form a fluidizable sealed experimental environment via said first conduit (61), said first return conduit (63); and/or the number of the groups of groups,

the second reagent bottle (41) adopts seal structure, second pipe (62) second return pipe (64) stretch into in second reagent bottle (41), work as second pump (82) during operation, first pump (81) are inoperative, test tube (21) with second reagent bottle (41) are via second pipe (62) second return pipe (64) form circulated sealed experimental environment.

56. A pathological tissue producing device according to claim 52, wherein a plurality of first reagent bottles (31) are arranged in the first reagent cartridge (3), wherein at least one first reagent bottle adopts a sealing structure;

the pathological tissue making device is provided with a plurality of first conduits (61) which are respectively connected to the plurality of first reagent bottles (31) from the test tubes (21) in the tissue bin (2), wherein the plurality of first conduits (61) are converged to the test tubes (21) through a shunt, the shunt can independently control the on-off of any first conduit (61), the test tubes (21) are dispersed to the plurality of first return conduits (63) through a return shunt, and the return shunt can independently control the on-off of any first return conduit (63).

57. A pathological tissue producing device according to claim 52, wherein said first catheter (61) has at one end of said tissue magazine (2) a respective one or more first tube-end branch catheters connected to said one or more tube sets, respectively.

58. A pathological tissue producing device according to claim 52, having a respective one or more first return conduits (63) connected to the one or more groups of tubes, respectively.

59. A pathological tissue producing device according to claim 52, wherein said first conduit (61) is connected to said group of test tubes at one end located in said tissue magazine (2), and said first return conduit (63) is connected to said group of test tubes at one end located in said tissue magazine (2).