CN115611965A - Multi-channel polypeptide synthesizer and synthesis method thereof - Google Patents
Multi-channel polypeptide synthesizer and synthesis method thereof Download PDFInfo
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- CN115611965A CN115611965A CN202211165443.7A CN202211165443A CN115611965A CN 115611965 A CN115611965 A CN 115611965A CN 202211165443 A CN202211165443 A CN 202211165443A CN 115611965 A CN115611965 A CN 115611965A
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- 229920001184 polypeptide Polymers 0.000 title claims abstract description 35
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 35
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 35
- 238000001308 synthesis method Methods 0.000 title claims abstract description 9
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 88
- 238000006243 chemical reaction Methods 0.000 claims abstract description 87
- 238000005070 sampling Methods 0.000 claims abstract description 76
- 238000004140 cleaning Methods 0.000 claims abstract description 37
- 238000003860 storage Methods 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 32
- 239000000843 powder Substances 0.000 claims description 26
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- 230000015572 biosynthetic process Effects 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 7
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
- C07K1/045—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers using devices to improve synthesis, e.g. reactors, special vessels
Abstract
The invention discloses a multichannel polypeptide synthesizer and a synthesis method thereof, and the multichannel polypeptide synthesizer comprises a horizontal base, a vertical mounting seat, a sampling module, a reaction module, a cleaning module and a reagent storage module, wherein the vertical mounting seat is fixedly arranged at the rear part of the horizontal base, the sampling module comprises a horizontal moving rail, a sampling seat and a sampling needle, the reaction module comprises at least four groups of reaction tubes, a rotating seat and a miniature multichannel plunger pump, the cleaning module comprises a cleaning tank and a cleaning tube, the reagent storage module comprises a first kit, a second kit, a third kit and a cover taking assembly, and the cover taking assembly is arranged on the vertical mounting seat above the first kit, the second kit and the third kit. The reagent feeding device has the advantages that after the reagent is placed in the early stage, the reagent can be fed by controlling the sampling module and the cover taking component, so that the manual sample feeding in midway is not needed, and the whole cleaning is simple.
Description
Technical Field
The invention relates to the technical field of biological pharmacy, in particular to a multichannel polypeptide synthesizer and a synthesis method thereof.
Background
The polypeptide is a bioactive substance related to various cell functions in organisms, the molecular structure of the polypeptide is between that of amino acid and protein, and the polypeptide is a compound formed by combining a plurality of amino acids through peptide bonds according to a certain arrangement sequence. Polypeptides are a general term for bioactive substances related to various cellular functions in the body of an organism, and are often used in the fields of functional analysis, antibody research, particularly drug development and the like. Polypeptide synthesis is a solid phase synthesis, generally ordered from the C-terminus (amino terminus) to the N-terminus (carboxyl terminus). The synthesis of polypeptides in the past has been carried out in solution and is referred to as a liquid phase synthesis. Since Merrifield successfully develops a solid-phase polypeptide synthesis method in 1963, the solid-phase method becomes a common technology in polypeptide and protein synthesis today through continuous improvement and perfection, and the method has incomparable advantages compared with the classical liquid-phase synthesis method, thereby greatly reducing the difficulty of product purification in each step.
The advent of polypeptide synthesizers has greatly facilitated the development of polypeptide science. In turn, with the development of polypeptide science, scientists have also made higher demands on the synthesizer, thereby driving the development of the synthesizer.
The full automation of the synthesizer can greatly reduce the labor force of scientific research personnel and improve the scientific research efficiency, and the synthesizer is a great direction for the development of future synthesizers.
However, the existing polypeptide synthesizer cannot realize complete automation, needs an experimenter to manually add samples halfway, is relatively complex in the subsequent cleaning process, needs manual weighing and adding of powder reagents, and cannot realize fine automation.
Disclosure of Invention
The invention aims to provide a multi-channel polypeptide synthesizer and a synthesis method thereof.
The technical purpose of the invention is realized by the following technical scheme:
a multi-channel polypeptide synthesizer is characterized by comprising a horizontal base, a vertical mounting seat, a sampling module, a reaction module, a cleaning module and a reagent storage module, wherein the vertical mounting seat is fixedly mounted at the rear of the horizontal base, the sampling module comprises a horizontal moving rail, a sampling seat and a sampling needle, the horizontal moving rail is horizontally embedded and fixedly mounted above the vertical mounting seat, one end of the sampling seat is arranged on the horizontal moving rail in a sliding manner through a first linear motor, the sampling needle is at least provided with four groups, the sampling needle is mounted inside the sampling seat in parallel, the reaction module comprises at least four groups of reaction tubes, rotary seats and micro multi-channel plunger pumps, the upper end and the lower end of each reaction tube are fixedly mounted on the vertical mounting seat below the horizontal moving rail through two groups of clamping seats respectively, the rotary seats are fixedly clamped and mounted in the middle of the reaction tubes, the inner side of the rotary seat is fixedly connected with a rotary motor inside the vertical installation seat, the miniature multi-channel plunger pump is fixedly installed on the vertical installation seat between the reaction tubes, a connecting port of the miniature multi-channel plunger pump is connected with the bottom of the reaction tubes through a sealing connecting seat in a sealing manner, the miniature multi-channel plunger pump is communicated with a nitrogen tank and a pure water tank, the cleaning module comprises a cleaning tank and a cleaning tube, the cleaning tank is installed on the right side of the horizontal base, the pure water tank is fixedly installed on the top of the vertical installation seat, the bottom of the pure water tank is communicated with the bottom of the cleaning tank through the cleaning tube, electromagnetic valves are all installed on the cleaning tube, the reagent storage module comprises a first reagent kit, a second reagent kit, a third reagent kit and a cover taking assembly, the first reagent kit, the second reagent kit and the third reagent kit are respectively and sequentially clamped and fixedly installed on the left side of the horizontal base, the cover taking component is arranged on the vertical installation seats above the first kit, the second kit and the third kit.
Preferably, the corresponding every group sampling needle all is provided with a spout in the sample seat, spout top level is provided with a sample track, it is provided with second linear electric motor to slide on the sample track, the last fixed centre gripping of second linear electric motor installs the lifing beam of a vertical setting, sampling needle fixed mounting is in the lifing beam front end.
Preferably, the needle calibers of at least four groups of the sampling needles are different, so that reagents in different states can be transferred.
Preferably, the rubber buffer is installed at the reaction tube top, be provided with an exhaust branch pipe in the reaction tube top slant, exhaust branch pipe's exit seal installation has an one-way pneumatic valve, the reaction tube inner wall is provided with an anti-overflow in exhaust branch pipe below is fixed and is kept off the ring, the anti-overflow keeps off the inner circle height that the ring is less than the outer lane height, the anti-overflow keeps off the ring and sets up for slant downwards.
Preferably, be provided with a plurality of reagent holding tanks in the first kit, the reagent bottle of having the screw thread lid has been placed in the reagent holding tank, be provided with three insulation tanks of group in the second kit, the insulation tank inner wall closes the heat transfer with the reagent bottle, the insulation tank outside is provided with annular runner, annular runner bottom is the inclined plane setting, annular runner bottom inclined plane upper end is provided with the water inlet, annular runner bottom inclined plane lower extreme is provided with the delivery port, annular runner inboard top is provided with the liquid level inductor, the water inlet all is connected with the hot and cold water all-in-one with the delivery port, the solenoid valve is all installed with delivery port department to the water inlet.
Preferably, the third kit includes accurate electronic scale, magnetism and inhales frame and powder bottle, the bottle lid top of powder bottle is provided with the lid groove of getting that is central symmetry setting, the bottle lid outside of powder bottle is provided with an annular magnetism and inhales rubber, be provided with the multiunit opening in the magnetism frame, the inboard top of opening is provided with multiunit annular electromagnet, the annular electromagnet bottom is inhaled the rubber top with annular magnetism and is passed through lug and recess cooperation spacing connection.
Preferably, get the lid subassembly including getting lid translation screw rod, getting lid seat, rotating the seat, adjusting seat, first rotating electrical machines and second rotating electrical machines, first rotating electrical machines bottom fixed mounting is on getting the lid seat, it all is provided with the transmission tooth's socket with the regulation seat outside to rotate the seat, the output of first rotating electrical machines passes through band pulley, drive belt and is connected with the transmission tooth's socket that rotates the seat outside, it connects in sample lid bottom to rotate the seat, it is provided with the spacing groove of array centrosymmetry on the seat to rotate, spacing inslot direction slip is provided with annular centering centre gripping arm, annular centering centre gripping arm inboard is provided with the arc arch, it installs in rotating the seat middle part to adjust the seat rotation, it is provided with array guide way to link up on the regulation seat, annular centering centre gripping arm inboard is provided with the guide block with the guide way, second rotating electrical machines fixed mounting is on rotating the seat, the output of second rotating electrical machines passes through band pulley, drive belt and is connected with the transmission tooth's socket that adjusts the seat outside, it installs telescopic cylinder to rotate the seat top, telescopic cylinder bottom fixed mounting has third linear electrical machines and then realizes displacement around the third linear electrical machines rear end connection slider, it realizes horizontal migration in the horizontal movement screw rod to get the horizontal movement.
Preferably, reaction tube inner wall below is provided with several sets of parallel arrangement's perpendicular groove, reaction tube inner wall below is provided with a separation blade, separation blade and reaction tube inner wall interference sliding connection, the separation blade is outer along being provided with the arch of a perpendicular groove gomphosis, it sets up the guiding gutter of an oblique downward setting still to sink in below the perpendicular groove.
Preferably, the vertical mounting seat is provided with a reaction prompt lamp above the reaction tube, and the inner side of the second kit is also provided with a reaction prompt lamp at each group of heat preservation grooves.
A method for synthesizing multichannel polypeptide, which is characterized by comprising the following steps: the method comprises the following steps:
step 1: taking reagent bottles required by the reaction, respectively putting the reagent bottles into a first reagent kit, a second reagent kit and a third reagent kit, putting reagents required to be stored at a specific temperature into the second reagent kit, installing powder bottles in a magnetic suction frame of the third reagent kit in a magnetic suction mode, and upwards and hermetically installing a blocking piece in the inner wall of a reaction tube at the top of a vertical groove;
step 2: when the first reagent box and the second reagent box need to be sampled, after the reagent bottle is subjected to cover taking through the cover taking component, the reagent bottle after the cover taking is accurately sampled through the sampling module, and the bottle cover is put back through the cover taking component after the sampling is finished;
and 3, step 3: after sampling is finished, the rear leg of the sampling needle in the sampling module retracts and penetrates into the rubber plug at the top of the reaction tube, so that a reagent is injected into the reaction tube, and the sampling needle needs to enter a cleaning tank to clean the sampling needle for multiple times after sampling injection is finished each time;
and 4, step 4: after the reactants are added, the reaction tube is driven by the rotating seat to rotate at a certain angle, so that the reagents are contacted more completely;
and 5: after the reaction is finished, generating suction force through a miniature multi-channel plunger pump, moving the blocking piece downwards under the pressure of the suction force, and after the blocking piece descends to the upper end of the diversion trench, collecting or discharging a product or waste liquid after the reaction through the miniature multi-channel plunger pump;
step 6: after the product or waste liquid in the reaction tube is completely discharged, pure water can be pumped in through the miniature multi-channel plunger pump, and the reaction tube is cleaned for multiple times through the rotating seat, so that multi-channel polypeptide synthesis can be finally realized.
In conclusion, the beneficial effects of the invention are as follows:
1. according to the invention, the samples in different states can be absorbed through the plurality of groups of sampling needles, so that the situation that the samples need to be manually added in the synthesis process is avoided.
2. The invention is provided with at least four groups of reaction tubes, can realize the synchronous operation of a plurality of groups of experiments, and simultaneously, the invention is communicated with a nitrogen tank and a pure water tank through a miniature multi-channel plunger pump, can finish the discharge of waste liquid or the collection of reaction liquid, and realizes the reaction automation through a rotary seat.
3. The invention is provided with a plurality of groups of reagent box channels and the cover taking component, meets the placement of common reagents, reagents needing to be stored under specific stability and powder, and simultaneously completes the opening and closing of the bottle cover through the cover taking component, thereby realizing the sampling automation.
4. The invention cleans the sampling needle by the action of gravity and the control of pure water by the electromagnetic valve, thereby avoiding the cross contamination of reagents.
5. The invention arranges a plurality of groups of sampling needles in the sampling module, and can simultaneously aim at different sampling types: aqueous solution, organic solvent or powder adopt different bores to realize accurately taking to different reagents, realize simultaneously through getting the lid subassembly that full-automatic getting is covered and is added with the lid, need not artifical the participation, take a sample simultaneously and accomplish back a lot of and wash, avoid taking place reagent cross contamination, the reaction tube can realize shaking evenly, the automatic collection and the cleaning function of reactant, degree of automation is higher.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic diagram of the sampling module structure of the present invention;
FIG. 3 is a schematic top view of the magnetic rack of the present invention;
FIG. 4 is a schematic view of the connection structure of the magnetic rack and the powder bottle of the present invention;
FIG. 5 is a schematic top view of a third reagent cartridge according to the present invention;
FIG. 6 is a schematic view of the internal structure of the annular flow passage of the present invention;
FIG. 7 is a schematic view of the internal structure of the reaction tube of the present invention;
FIG. 8 is a schematic bottom view of the access cover assembly of the present invention;
FIG. 9 is a schematic view of the front side of the cover removing assembly of the present invention;
FIG. 10 isbase:Sub>A schematic view of the structure of FIG. 9 at A-A;
fig. 11 is a schematic view of the structure at B-B in fig. 9.
Detailed Description
The following further describes the embodiments of the present invention with reference to the drawings, and the present embodiment is not to be construed as limiting the invention.
As shown in fig. 1 to 11, a multi-channel polypeptide synthesizer comprises a horizontal base 1, a vertical mounting seat 3, a sampling module 4, a reaction module 5, a cleaning module 6 and a reagent storage module, wherein the vertical mounting seat 3 is fixedly mounted at the rear of the horizontal base 1, the sampling module 4 comprises a horizontal moving rail 7, a sampling seat 8 and a sampling needle 9, the horizontal moving rail 7 is horizontally embedded and fixedly mounted above the vertical mounting seat 3, one end of the sampling seat 8 is slidably arranged on the horizontal moving rail 7 through a first linear motor 10, at least four groups of sampling needles 9 are arranged, the sampling needles 9 are mounted in the sampling seat 8 in parallel, the reaction module 5 comprises at least four groups of reaction tubes 11, a rotary seat 12 and a micro multi-channel plunger pump 13, the upper and lower ends of the reaction tubes 11 are respectively and fixedly mounted on the vertical mounting seat 3 below the horizontal moving rail 7 through two groups of clamping seats 14, the rotary seat 12 is fixedly clamped and installed in the middle of the reaction tube 11, a rotary motor is fixedly connected to the inner side of the rotary seat 12 in the vertical installation seat 3, the micro multi-channel plunger pump 13 is fixedly installed on the vertical installation seat 3 between the reaction tubes 11, a connecting port of the micro multi-channel plunger pump 13 is in sealing connection with the bottom of the reaction tubes 11 through a sealing connecting seat 15 and a pipeline 16, the micro multi-channel plunger pump 13 is communicated with a nitrogen tank and a pure water tank 17, the cleaning module 6 comprises a cleaning tank 18 and a cleaning tube 19, the cleaning tank 18 is installed on the right side of the horizontal base 1, the pure water tank 17 is fixedly installed on the top of the vertical installation seat 3, the bottom of the pure water tank 17 is communicated with the bottom of the cleaning tank 18 through the cleaning tube 19, electromagnetic valves 20 are installed on the cleaning tube 19, the reagent storage module comprises a first reagent box 21, a second reagent box 22, a third reagent box 23 and a cover taking assembly 24, the first reagent storage module 21, the second kit 22 and the third kit 23 are respectively and sequentially clamped and fixedly mounted on the left side of the horizontal base 1, and the cover taking assembly 24 is mounted on the vertical mounting seat 3 above the first kit 21, the second kit 22 and the third kit 23.
Correspond every group sampling needle 9 in the sample seat 8 and all be provided with a spout 25, spout 25 top level is provided with a sample track 26, it is provided with second linear electric motor 27 to slide on the sample track 26, the lifting beam 28 of a vertical setting is installed to fixed centre gripping on the second linear electric motor 27, 9 fixed mounting in the 28 front ends of lifting beam, the syringe needle bore of at least four groups sampling needle 9 is all inequality, and then move the reagent of getting different states, every group sampling needle 9 can realize fore-and-aft removal through second linear electric motor 27, realize the removal of left right direction through first linear electric motor 10, lifting beam 28 then can realize reciprocating and then the absorption and the injection of realization sample of sampling needle 9.
A plurality of reagent storage grooves 34 are arranged in a first reagent kit 21, reagent bottles 33 with threaded covers are placed in the reagent storage grooves 34, three groups of heat preservation grooves 35 are arranged in a second reagent kit 22, the inner walls of the heat preservation grooves 35 are attached to the reagent bottles 33 to transfer heat, an annular flow channel 36 is arranged on the outer side of the heat preservation grooves 35, the bottom of the annular flow channel 36 is arranged in an inclined plane, a water inlet 37 is formed in the upper end of the inclined plane of the bottom of the annular flow channel 36, a water outlet 38 is formed in the lower end of the inclined plane of the bottom of the annular flow channel 36, a liquid level sensor 61 is arranged above the inner side of the annular flow channel 36, the water inlet 37 and the water outlet 38 are both connected with a cold and hot water integrated machine, an electromagnetic valve 20 is installed at the water inlet 37 and the water outlet 38, the electromagnetic valve 20 at the water outlet 38 is controlled to be opened through the liquid level sensor 61, namely, the electromagnetic valve 20 at the water outlet 38 is opened under the condition that a certain liquid level is reached, the water level, the water circulation in the annular flow channel 36 is realized, and the temperature stability in the heat preservation grooves 35 is further realized.
The cap taking assembly 24 comprises a cap taking translation screw 45, a cap taking seat 46, a rotating seat 47, an adjusting seat 48, a first rotating motor 49 and a second rotating motor 50, the bottom of the first rotating motor 49 is fixedly arranged on the cap taking seat 46, the outer sides of the rotating seat 47 and the adjusting seat 48 are both provided with a transmission tooth groove, the output end of the first rotating motor 49 is connected with the transmission tooth groove on the outer side of the rotating seat 47 through a belt wheel and a transmission belt, the rotating seat 47 is rotatably connected with the bottom of the sampling cap, the rotating seat 47 is provided with a plurality of groups of centrosymmetric limiting grooves 51, the limiting grooves 51 are provided with annular centering clamping arms 52 in a guiding and sliding manner, the inner sides of the annular centering clamping arms 52 are provided with arc-shaped bulges, the adjusting seat 48 is rotatably arranged in the middle part of the rotating seat 47, the adjusting seat 48 is provided with a plurality of groups of guide grooves 53 in a penetrating manner, the inner sides of the annular centering clamping arms 52 and the guide grooves 53 are provided with guide blocks 54, the second rotating seat 50 is fixedly arranged on the rotating seat 47, the output end of the second rotating motor 50 is connected with the transmission tooth groove on the outer side of the adjusting seat 48 through a belt, a belt wheel and a telescopic cylinder 55 is arranged above the rotating seat, the rotating seat 55, the bottom of the telescopic cylinder 55 is fixedly arranged to realize the horizontal translation screw 45 and further realize the horizontal translation slider 57 connected with a third linear motor 56, and the horizontal translation screw 45, and the horizontal translation slider 57 on the rear end of the third linear translation screw 45, through the rotatory seat 47 that rotates of second rotating electrical machines 50, the realization is pressed from both sides the centering of annular centering centre gripping arm 52 and is embraced the distance, the inboard arc arch of annular centering centre gripping arm 52 can stably press from both sides the screw cap of reagent bottle 33 and embrace, follow-up rethread first rotating electrical machines 49 rotates gets lid seat 46, thereby realize accomplishing taking off and covering of screw cap to the rotation of the screw cap of reagent bottle 33, first rotating electrical machines 49 all can realize the rotation of positive and negative direction with second rotating electrical machines 50.
A multichannel polypeptide synthesis method comprises the following steps:
step 1: the reagent bottles 33 required by the reaction are respectively put into the first reagent kit 21, the second reagent kit 22 and the third reagent kit 23, the reagent required to be stored at a specific temperature is placed in the second reagent kit 22, the powder bottle 41 is installed in the magnetic suction frame 40 of the third reagent kit 23 in a magnetic suction mode, and meanwhile, the blocking piece 59 is upwards and hermetically installed in the inner wall of the reaction tube 11 at the top of the vertical groove 58.
Step 2: need be to first kit 21, when taking a sample in the second kit 22, get the lid back to reagent bottle 33 through getting lid subassembly 24, reagent bottle 33 after accomplishing to get the lid is taken a sample through sampling module 4 is accurate, the rethread lid subassembly 24 of getting after the sample is accomplished is put back the bottle lid, when needs take a sample to powder bottle 41 in the third kit 23, get the lid to powder bottle 41 through getting lid subassembly 24 earlier, the demagnetization of cutting off the power supply to the electro-magnet on the powder bottle 41 that needs the sample again, powder bottle 41 drops to accurate electronic scale 39 on, the great sample needle 9 of bore absorbs the powder on the rethread sampling module 4.
And step 3: after sampling, the rear leg of the sampling needle 9 in the sampling module 4 retracts to penetrate through the rubber plug 29 at the top of the reaction tube 11, so that the reagent is injected into the reaction tube 11, and the sampling needle 9 needs to be washed for multiple times in the washing tank 18 after sampling and injecting are completed each time.
And 4, step 4: after the reactant is added, the reaction tube 11 is driven to rotate by a certain angle through the rotating seat 47, so that the contact of the reagent is more complete.
And 5: after the reaction is finished, a suction force is generated through the miniature multi-channel plunger pump 13, the blocking piece 59 moves downwards under the pressure of the suction force, and after the blocking piece 59 descends to the upper end of the guide groove 60, products or waste liquid after the reaction can be collected or discharged through the miniature multi-channel plunger pump 13.
Step 6: after the product or waste liquid in the reaction tube 11 is completely discharged, pure water can be pumped in through the miniature multi-channel plunger pump 13, and the reaction tube 11 is cleaned for a plurality of times through the rotary seat 12, so that multi-channel polypeptide synthesis can be finally realized.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes may be made and equivalents may be substituted without departing from the spirit and scope of the invention.
Claims (10)
1. A multi-channel polypeptide synthesizer is characterized by comprising a horizontal base, a vertical mounting seat, a sampling module, a reaction module, a cleaning module and a reagent storage module, wherein the vertical mounting seat is fixedly mounted at the rear of the horizontal base, the sampling module comprises a horizontal moving rail, a sampling seat and a sampling needle, the horizontal moving rail is embedded and fixedly mounted above the vertical mounting seat, one end of the sampling seat is arranged on the horizontal moving rail in a sliding manner through a first linear motor, the sampling needle is at least provided with four groups, the sampling needle is mounted inside the sampling seat in parallel, the reaction module comprises at least four groups of reaction tubes, rotary seats and a micro multi-channel plunger pump, the upper end and the lower end of each reaction tube are fixedly mounted on the vertical mounting seat below the horizontal moving rail through two groups of clamping seats respectively, the rotary seats are fixedly clamped and mounted in the middle of the reaction tubes, the rotary seats are fixedly connected with rotary motors inside the vertical mounting seat, the micro multi-channel plunger pump is fixedly mounted on the vertical mounting seat between the reaction tubes, a connecting port of the micro multi-channel plunger pump is connected with the bottoms of the reaction tubes through a sealing connecting seat and a pipeline, the multi-channel plunger pump is provided with a nitrogen tank, the cleaning tank and a cleaning module, the cleaning kit comprises a third cleaning tank, a third cleaning tank and a third cleaning tank, a third cleaning kit, a third cleaning tank and a third cleaning kit, a third cleaning tank are mounted on a third cleaning tank, the cover taking component is arranged on the vertical installation seats above the first kit, the second kit and the third kit.
2. The multi-channel polypeptide synthesizer as claimed in claim 1, wherein: the sampling seat is internally provided with a sliding groove corresponding to each group of sampling needles, a sampling track is horizontally arranged above the sliding groove, a second linear motor is arranged on the sampling track in a sliding mode, a lifting beam with a vertical setting is fixedly clamped on the second linear motor, and the sampling needles are fixedly installed at the front end of the lifting beam.
3. The multi-channel polypeptide synthesizer as claimed in claim 1, wherein: the needle head calibers of at least four groups of sampling needles are different, so that reagents in different states can be transferred.
4. The multi-channel polypeptide synthesizer as claimed in claim 1, wherein: the rubber buffer is installed at the reaction tube top, be provided with an exhaust branch pipe in the reaction tube top slant, exhaust branch pipe's exit seal mounting has an one-way pneumatic valve, the reaction tube inner wall is fixed in exhaust branch pipe below and is provided with an anti-overflow and keeps off the ring, the anti-overflow keeps off the inner circle height that the ring is less than the outer lane height, the anti-overflow keeps off the ring and sets up for slant downwards.
5. The multi-channel polypeptide synthesizer as claimed in claim 1, wherein: the reagent bottle heat preservation kit comprises a first kit and is characterized in that a plurality of reagent storage tanks are arranged in the first kit, reagent bottles with threaded covers are placed in the reagent storage tanks, three groups of heat preservation tanks are arranged in the second kit, the inner walls of the heat preservation tanks and the reagent bottles are in heat transfer in a matching mode, annular flow channels are arranged on the outer sides of the heat preservation tanks, the bottoms of the annular flow channels are inclined planes, water inlets are formed in the upper ends of the inclined planes of the bottoms of the annular flow channels, water outlets are formed in the lower ends of the inclined planes of the bottoms of the annular flow channels, liquid level sensors are arranged above the inner sides of the annular flow channels, the water inlets and the water outlets are connected with a cold and hot water all-in-one machine, and electromagnetic valves are installed at the water inlets and the water outlets.
6. The multi-channel polypeptide synthesizer as claimed in claim 1, wherein: the third kit includes accurate electronic scale, magnetism and inhales frame and powder bottle, the bottle lid top of powder bottle is provided with the lid groove of getting that is central symmetry setting, the bottle lid outside of powder bottle is provided with an annular magnetism and inhales rubber, be provided with the multiunit opening in the magnetism frame, the inboard top of opening is provided with multiunit annular electromagnet, the annular electromagnet bottom is inhaled rubber top with annular magnetism and is passed through lug and recess cooperation spacing connection.
7. The multi-channel polypeptide synthesizer as claimed in claim 1, wherein: get the lid subassembly including getting lid translation screw rod, getting lid seat, rotating seat, regulation seat, first rotating electrical machines and second rotating electrical machines, first rotating electrical machines bottom fixed mounting is on getting the lid seat, it all is provided with the transmission tooth's socket with the regulation seat outside to rotate the seat, the output of first rotating electrical machines passes through band pulley, drive belt and rotates the transmission tooth's socket in the seat outside and be connected, it connects in sample lid bottom to rotate the seat, it is provided with the spacing groove of array centrosymmetry on the seat to rotate, spacing inslot direction slip is provided with annular centering centre gripping arm, annular centering centre gripping arm inboard is provided with the arc arch, it installs in the rotation seat middle part to adjust the seat rotation, it is provided with the array guide way to link up on the regulation seat, be provided with the guide block in annular centering centre gripping arm inboard and the guide way, second rotating electrical machines fixed mounting is on rotating the seat, the output of second rotating electrical machines passes through band pulley, drive belt and is connected with the transmission tooth's socket outside, rotate the seat top and install telescopic cylinder, telescopic cylinder bottom fixed mounting has third linear electrical machines and then realizes displacement around, third linear machines rear end connection a horizontal slider, horizontal sliding block is in the translation lid in the horizontal movement in the horizontal sliding block.
8. The multi-channel polypeptide synthesizer as claimed in claim 1, wherein: reaction inside pipe wall below is provided with several sets of parallel arrangement's perpendicular groove, reaction inside pipe wall below is provided with a separation blade, separation blade and reaction inside pipe wall interference sliding connection, the separation blade is outer along being provided with the arch of a perpendicular groove gomphosis, it sets up the guiding gutter of an oblique downward setting still to sink in the groove below to erect.
9. The multi-channel polypeptide synthesizer as claimed in claim 1, wherein: the vertical mounting seat is provided with reaction prompt lamps above the reaction tubes, and the inner side of the second kit is also provided with reaction prompt lamps at the position of each group of heat preservation grooves.
10. A multichannel polypeptide synthesis method according to claims 1 to 9, characterized in that: the method comprises the following steps:
step 1: taking reagent bottles required by the reaction, respectively putting the reagent bottles into a first reagent kit, a second reagent kit and a third reagent kit, putting reagents required to be stored at a specific temperature into the second reagent kit, installing powder bottles in a magnetic suction frame of the third reagent kit in a magnetic suction mode, and upwards and hermetically installing a blocking piece in the inner wall of a reaction tube at the top of a vertical groove;
step 2: when the first reagent box and the second reagent box need to be sampled, after the reagent bottle is subjected to cover taking through the cover taking component, the reagent bottle after the cover taking is accurately sampled through the sampling module, and the bottle cover is put back through the cover taking component after the sampling is finished;
and step 3: after sampling is finished, the rear leg of the sampling needle in the sampling module retracts to penetrate through the rubber plug at the top of the reaction tube, so that a reagent is injected into the reaction tube, and the sampling needle needs to enter a cleaning tank to clean the sampling needle for multiple times after sampling injection is finished each time;
and 4, step 4: after the reactants are added, the reaction tube is driven by the rotating seat to rotate at a certain angle, so that the reagents are contacted more completely;
and 5: after the reaction is finished, generating suction force through a miniature multi-channel plunger pump, moving the blocking piece downwards under the pressure of the suction force, and after the blocking piece descends to the upper end of the diversion trench, collecting or discharging a product or waste liquid after the reaction through the miniature multi-channel plunger pump;
step 6: after the product or waste liquid in the reaction tube is completely discharged, pure water can be pumped in through the miniature multi-channel plunger pump, and the reaction tube is cleaned for multiple times through the rotating seat, so that multi-channel polypeptide synthesis can be finally realized.
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