CN113866444A - Automatic sample adding and mixing device for pretreatment of spectrophotometry - Google Patents
Automatic sample adding and mixing device for pretreatment of spectrophotometry Download PDFInfo
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- CN113866444A CN113866444A CN202111121101.0A CN202111121101A CN113866444A CN 113866444 A CN113866444 A CN 113866444A CN 202111121101 A CN202111121101 A CN 202111121101A CN 113866444 A CN113866444 A CN 113866444A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00584—Control arrangements for automatic analysers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1002—Reagent dispensers
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00346—Heating or cooling arrangements
- G01N2035/00356—Holding samples at elevated temperature (incubation)
- G01N2035/00376—Conductive heating, e.g. heated plates
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
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Abstract
The invention provides an automatic sample adding and mixing device for pretreatment of spectrophotometry, which mainly comprises a base, a sample adding and sampling device, a transmission and mixing device, a weighing device, a reagent adding device, a cleaning device, a control system and a control terminal, wherein the sample adding and sampling device is used for adding a buffering agent and sampling a sample cup which is added with the buffering agent and is uniformly mixed; the control terminal mainly exists in the form of running control software in a network computer. The sample adding and sampling device, the transmission blending device, the weighing device, the reagent adding device, the cleaning device, the control system and the control terminal are matched for use, so that automatic sample adding of a cuvette of a pretreatment part in a spectrophotometry is realized, the efficiency and the accuracy are high, the error is small, the cost of manual sample adding of professional experimenters is reduced, and the cost is saved.
Description
Technical Field
The invention mainly relates to the technical field of detection equipment, in particular to an automatic sample adding and mixing device for pretreatment by a spectrophotometry method.
Background
The detection technology is one of the indispensable means of scientific experiments, the commonly used ultraviolet spectrophotometry is a qualitative, quantitative and structural analysis method established according to the absorption characteristics of substance molecules to electromagnetic waves with different ranges of wavelengths, and a spectrophotometer is used as an optical detection device and is often used for detecting the content of each element of an analysis sample.
The spectrophotometry comprises two parts of pretreatment and detection; the workload of the pretreatment part accounts for about 80% of the whole process, however, in the spectrophotometric detection experiment, the whole pretreatment work contents such as sample weighing, sample liquid adding, uniform oscillation mixing, sample extraction, reagent adding and the like still need to be completed through manual operation, and because the pretreatment part has more work links, the manual operation is not only low in efficiency, but also inevitably causes operation errors, and further influences the on-machine detection result. Therefore, there is a need for an automatic sample-adding and mixing device for pretreatment, which can automatically complete the work content of pretreatment in spectrophotometry, so as to achieve the purpose of saving labor and time and saving cost.
Disclosure of Invention
The invention provides an automatic sample adding and mixing device for pretreatment in a spectrophotometry, aiming at solving the technical problems that the manual sample adding method is low in efficiency, poor in accuracy, error exists in a detection result, labor cost is high, and automatic detection cannot be realized in the spectrophotometry.
The technical scheme provided by the invention is as follows:
the invention provides an automatic sample adding and mixing device for pretreatment of spectrophotometry, which comprises a base, a power supply arranged on the base, a transmission mixing device for mixing samples, a sample adding and sampling device for adding a buffering agent, a reagent adding device for adding an experimental reagent, a weighing device for measuring the whole weight of a sample cup, a cleaning device for cleaning a sample adding and sampling needle and a reagent needle, a control terminal for collecting data and a control system arranged above the base, wherein the power supply is arranged on the base; the transmission blending device is fixedly arranged on the base through a support column; the sample adding and sampling device, the reagent adding device, the weighing device and the cleaning device are arranged around the transmission blending device; the control system is used for controlling the transmission blending device, the sample adding and sampling device, the reagent adding device, the weighing device and the cleaning device, and is also used for receiving an instruction sent by the control terminal; the control terminal is in the form of running control software in a network computer or a mobile phone.
Preferably, the transmission blending device comprises a first stepping motor, a first transmission belt, a rotating shaft, a tray and a tray bracket; the tray support comprises the supporting column and a fixed platform; the supporting column is vertically arranged on the base; the fixed platform is horizontally and fixedly arranged at the top end of the supporting column; the center of the fixed platform is provided with a shaft hole; the rotating shaft penetrates through the shaft hole; a first bearing (not shown) is arranged in the shaft hole; the outer ring of the first bearing is embedded in the inner wall of the shaft hole; the inner ring of the first bearing is embedded in the surface of the rotating shaft; the tray is arranged in parallel to the fixed platform; the center of the tray is fixedly arranged at the top end of the rotating shaft; first step motor passes through "L type" mount and installs fixed platform's below, the vertical setting of its drive shaft, this drive shaft pass through first drive belt with the bottom transmission of pivot is connected.
Preferably, the tray is disc-shaped; a plurality of sample holes, a plurality of reagent holes and a plurality of cuvette holes are arranged on the surface of the disc-shaped tray in a penetrating way; the sample holes, the reagent holes and the cuvette holes are uniformly distributed around the axis of the center of the disc-shaped tray respectively; the distance from the center of the cuvette hole to the axis is a, the distance from the center of the sample hole to the axis is b, and the distance from the center of the reagent hole to the axis is c; a ≦ b ≦ c; a first connecting column is arranged on the lower bottom surface at the edge of the tray; the first connecting column is arranged perpendicular to the tray; the bottom end of the first connecting column is connected with an arc-shaped supporting plate; the arc-shaped supporting plate is arranged between the tray and the fixed platform, and the arc-shaped supporting plate is arranged right below all the reagent holes; the bottom of the tray is also provided with a second connecting column; the bottom end of the second connecting column is connected with an annular supporting plate; the annular supporting plate is arranged between the tray and the fixed platform, and the annular supporting plate is arranged under all the cuvette holes.
Preferably, the fixed platform is provided with a heating plate; a temperature sensor is arranged on the heating plate; a heating coil for heating is arranged in the heating plate; the heating plate is fixedly arranged at the center of the fixed platform; the center of the heating plate is provided with a through hole for the rotating shaft to pass through; the heating plate is provided with an annular groove for heating the cuvette; the annular supporting plate is arranged in the annular groove.
Preferably, the reagent adding device comprises a first mounting seat, a plunger pump, a second stepping motor, a first rotating rod, a first rotating arm and an L-shaped reagent needle; the first mounting seat comprises a first lower plate surface, a first supporting plate and a first upper plate surface; the first lower plate surface is fixedly arranged on the base; the first upper plate surface is arranged right above the first lower plate surface; the first supporting plate is used for connecting the first upper plate surface and the first lower plate surface; the second stepping motor is fixedly arranged at the bottom of the first upper plate surface, and a driving shaft of the second stepping motor penetrates through the first upper plate surface; the first rotating rod can rotatably penetrate through the first upper plate surface; the driving shaft of the second stepping motor is in transmission connection with the first rotating rod through a second transmission belt; a third stepping motor is arranged at the bottom of the first lower plate surface; a driving shaft of the third stepping motor penetrates through the first supporting plate and a first driving wheel is arranged at the tail end of the driving shaft; a first driven wheel is arranged right above the first driving wheel; the first driving wheel and the first driven wheel are in transmission connection through a third transmission belt; the second bearing is sleeved at the bottom end of the first rotating rod; the outer ring of the second bearing is fixedly provided with a first transmission plate; the first transmission plate is fixedly connected with one side of the third transmission belt; one end of the first rotating arm is fixedly arranged at the top end of the first rotating rod; the vertical section of the L-shaped reagent needle penetrates through the other end of the first rotating arm; a reagent tube penetrates through the first rotating rod; one end of the reagent tube is connected with the L-shaped transverse section of the reagent needle, and the other end of the reagent tube is connected to the plunger pump.
Preferably, the sample adding and sampling device comprises a second mounting seat, a peristaltic pump, a fourth stepping motor, a second rotating rod, a second rotating arm and an L-shaped sample adding and sampling needle; the second mounting seat comprises a second lower plate surface, a second supporting plate and a second upper plate surface; the second lower plate surface is fixedly arranged on the base; the second upper plate surface is arranged right above the second lower plate surface; the second supporting plate is used for connecting the second upper plate surface and the second lower plate surface; the fourth stepping motor is fixedly arranged at the bottom of the second upper plate surface, and a driving shaft of the fourth stepping motor penetrates through the second upper plate surface; the second rotating rod can rotatably penetrate through the second upper plate surface; a driving shaft of the fourth stepping motor is in transmission connection with the second rotating rod through a fourth transmission belt; a fifth stepping motor is arranged at the bottom of the second lower board surface; a driving shaft of the fifth stepping motor penetrates through the second supporting plate and a second driving wheel is arranged at the tail end of the driving shaft; a second driven wheel is arranged right above the second driving wheel; the second driving wheel and the second driven wheel are in transmission connection through a fifth transmission belt; the bottom end of the second rotating rod is sleeved with a third bearing; the outer ring of the third bearing is fixedly provided with a second transmission plate; the second transmission plate is fixedly connected with one side of the fifth transmission belt; one end of the second rotating arm is fixedly arranged at the top end of the second rotating rod; the vertical section of the L-shaped sample adding and sampling needle penetrates through the other end of the second rotating arm; a sample liquid pipe is arranged in the second rotating rod in a penetrating way; one end of the sample liquid pipe is connected with the L-shaped transverse section of the sample adding and sampling needle, and the other end of the sample liquid pipe is connected to the peristaltic pump.
Preferably, the weighing device comprises a scale pan, a pressure sensor and a screw motor; a screw rod of the screw rod motor is arranged along the vertical direction; a sliding block is arranged on a screw rod of the screw rod motor in a sliding way; the upper end of the sliding block is connected with a pressure sensor, and the upper end of the pressure sensor is connected with a scale pan; the fixed platform is provided with a sample groove through the plate surface; the scale pan penetrates through the sample groove; the scale plate is arranged right below the sample hole.
Preferably, the cleaning device comprises a cleaning pool arranged on the fixed platform, a first diaphragm pump and a second diaphragm pump arranged on the base; the first diaphragm pump is provided with a waste liquid pipe and a liquid discharge pipe; the waste liquid pipe is connected with the cleaning pool and the first diaphragm pump; one end of the liquid discharge pipe is connected with the first diaphragm pump, and the other end of the liquid discharge pipe is connected with the waste liquid pool;
the second diaphragm pump is provided with a water inlet pipe and a water outlet pipe; one end of the water inlet pipe is connected with the clean water tank, the other end of the water inlet pipe is connected with the second diaphragm pump, and the water outlet pipe is connected with the second diaphragm pump and the cleaning tank; the water outlet pipe is connected with a branch pipe; the branch pipe is provided with an electromagnetic valve; the tail end of the branch pipe is connected to the plunger pump; .
Preferably, the control system comprises a control mainboard and a data exchanger connected with the control mainboard; the control terminal is connected with the control main board in a wireless or electric connection mode; the data exchanger comprises a temperature control module and a driving module; the temperature control module is connected with the temperature sensor and is used for controlling the temperature of the heating plate; the first driving module is used for controlling the operation of the first stepping motor, the second stepping motor, the third stepping motor, the fourth stepping motor, the fifth stepping motor, the plunger pump, the peristaltic pump, the diaphragm pump and the screw rod motor.
The invention has the beneficial effects that:
the automatic sample adding and mixing device for the spectrophotometry pretreatment, provided by the invention, is used in cooperation with a sample adding and sampling device, a transmission and mixing device, a weighing device, a reagent adding device, a cleaning device, a control system and a control terminal; the automatic adding of the sample and the reagent of the cuvette of the pretreatment part in the spectrophotometry is realized, the efficiency and the accuracy are high, the error is small, the cost of the manual sample adding of professional experimenters is reduced, and the cost is saved.
Drawings
The invention and its features, aspects and advantages will become more apparent from reading the following detailed description of non-limiting embodiments with reference to the accompanying drawings. Like reference symbols in the various drawings indicate like elements. The drawings are not necessarily to scale, emphasis instead being placed upon illustrating the principles of the invention.
FIG. 1 is a perspective view of the present invention;
FIG. 2 is a perspective view of another embodiment of the present invention;
FIG. 3 is a front view of the present invention;
FIG. 4 is a top view of the present invention with respect to a stationary platen;
FIG. 5 is a perspective view of the weighing apparatus of the present invention;
FIG. 6 is a perspective view of the mixing device of the present invention;
FIG. 7 is a perspective view of a reagent adding apparatus according to the present invention;
Detailed Description
It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict. It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments according to the present application.
It will be understood that the terms "comprises" and/or "comprising," when used in this specification, specify the presence of stated features, steps, operations, devices, components, and/or combinations thereof.
As used herein, the terms "center," "upper," "lower," "left," "right," "vertical," "horizontal," "inner," "outer," and the like are used in the positional or orientational relationship illustrated in the figures to facilitate the description of the invention and to simplify the description, and are not intended to indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and are therefore not to be construed as limiting the invention.
The appearances of the terms first, second, and third, if any, are used for descriptive purposes only and are not intended to be limiting or imply relative importance.
Unless expressly stated or limited otherwise, the terms "mounted," "connected," and "connected" are intended to be inclusive and mean, for example, that they may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
The technical solutions in the embodiments of the present invention are described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. Thus, the following detailed description of the embodiments of the present invention, presented in the figures, is not intended to limit the scope of the invention, as claimed, but is merely representative of selected embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments of the invention without making creative efforts, belong to the protection scope of the invention.
Example 1
The method aims to solve the technical problems that in a spectrophotometry method, a manual sample adding method is low in efficiency, poor in accuracy, high in labor cost and incapable of achieving automatic detection, and detection results have errors.
The embodiment of the invention provides an automatic sample adding and mixing device for pretreatment in spectrophotometry, which comprises a base 1, a power supply 2 (used for supplying power to the whole device) arranged on the base 1, a transmission mixing device 3 used for mixing samples uniformly, a sample adding and sampling device 4 used for adding a buffering agent, a reagent adding device 5 used for adding an experimental reagent, a weighing device 6 used for measuring the whole weight of a sample cup, a cleaning device 7 used for cleaning a sample adding and sampling needle 406 and a reagent needle 506, a control terminal 8 used for collecting data and a control system 9 arranged above the base 1; the transmission blending device 3 is fixedly arranged on the base 1 through a support column 10; the sample adding and sampling device 4, the reagent adding device 5, the weighing device 6 and the cleaning device 7 are arranged around the transmission blending device 3; the control system 9 is used for controlling the transmission blending device 3, the sample adding and sampling device 4, the reagent adding device 5, the weighing device 6 and the cleaning device 7, and the control system 9 is also used for receiving an instruction sent by the control terminal 8; the control terminal 8 is in the form of operating control software in a network computer or a mobile phone.
In this embodiment, preferably, the transmission blending device 3 includes a first stepping motor 301, a first transmission belt 302, a rotating shaft 303, a tray 304 and a tray support 305; the tray support 305 includes a support column 10 and a fixed platform 11; the supporting column 10 is vertically arranged on the base 1; the fixed platform 11 is horizontally and fixedly arranged at the top end of the supporting column 10; the center of the fixed platform 11 is provided with a shaft hole; the rotating shaft 303 penetrates through the shaft hole; a first bearing is arranged in the shaft hole; the outer ring of the first bearing is embedded in the inner wall of the shaft hole; the inner ring of the first bearing is embedded on the surface of the rotating shaft 303, so that the tray 304 can rotate under the action of the first stepping motor 301, and the fixed platform 11 cannot; the tray 304 is arranged parallel to the fixed platform 11; the center of the tray 304 is fixedly arranged at the top end of the rotating shaft 303; the first stepping motor 301 is installed below the fixed platform 11 through an "L-shaped" fixed frame 12, a driving shaft of the first stepping motor is vertically arranged, the driving shaft is in transmission connection with the bottom end of the rotating shaft 303 through a first transmission belt 302, the first stepping motor 301 receives an instruction from the driving module 33, and drives the rotating shaft and the tray 303 installed on the rotating shaft to synchronously move through the first transmission belt 302; the driving wheel can be additionally arranged on the driving shaft, the driven wheel can be additionally arranged at the bottom of the rotating shaft, and the first transmission belt 302 can act on the driving wheel and the driven wheel to realize synchronous motion of the driving shaft and the rotating shaft.
In this embodiment, the tray 304 is preferably in the shape of a disk; the surface of the disc-shaped tray 304 is provided with a plurality of sample holes 13, a plurality of reagent holes 14 and a plurality of cuvette holes 15 in a penetrating manner, and the sample cups, the reagent bottles and the cuvettes are respectively placed in the sample holes 13, the reagent holes 14 and the cuvette holes 15; the sample holes 13, the reagent holes 14 and the cuvette holes 15 are respectively and uniformly distributed around the axis of the center of the disc-shaped tray 304; the distance from the center of the cuvette hole 15 to the axis is a, the distance from the center of the sample hole 13 to the axis is b, and the distance from the center of the reagent hole 14 to the axis is c; a is less than or equal to b is less than or equal to c, because in spectrophotometry experiments, the diameter of the sample cup is generally larger than the aperture of the cuvette, and to ensure that the number of the sample cups is consistent with that of the cuvettes, the sample hole 13 for placing the sample cup must be arranged at the outer circle of the tray 304, and the reagent hole 14 is arranged at the outermost circle of the tray 304 for matching with the reagent needle 506; the lower bottom surface at the edge of the tray 304 is provided with a first connecting column 16; the first connecting post 16 is disposed vertically to the tray 304; the bottom end of the first connecting column 16 is connected with an arc-shaped supporting plate 17; the arc supporting plate 17 is arranged between the tray 304 and the fixed platform 11, the arc supporting plate 17 is arranged under all the reagent holes 14, and the arc supporting plate 17 can be used for supporting each reagent bottle, so that each reagent bottle cannot fall down and is maintained at a corresponding position; the bottom of the tray 304 is also provided with a second connecting column 18; the bottom end of the second connecting column 18 is connected with an annular supporting plate 19; annular layer board 19 is established between tray 304 and fixed platform 11, and annular layer board 19 sets up under whole cell hole 15, and is similar with arc layer board 17's effect, adds annular tray 19 of establishing and can prevent that the cell from dropping.
In this embodiment, it is preferable that the heating plate 20 is provided on the fixed platform 11; a temperature sensor is arranged on the heating plate 20; a heating coil (not shown) for heating is provided in the heating pan 20; the heating plate 20 is fixedly arranged at the center of the fixed platform 11, the low environmental temperature in winter can influence the activity of various reagents, and in order to avoid the inconvenience brought to the spectrophotometry experiment by the environmental temperature, the heating plate cuvette is used for raising the temperature; the center of the heating plate 20 is provided with a through hole for the rotating shaft 303 to pass through; the heating plate 20 is provided with an annular groove 21 for heating the cuvette; the annular supporting plate 19 is arranged in the annular groove 21, and each cuvette is heated and insulated in the groove of the annular groove 21.
In this embodiment, the reagent adding apparatus 5 preferably includes a first mounting seat 501, a plunger pump 502, a second stepping motor 503, a first rotating rod 504, a first rotating arm 505, and an "L-shaped" reagent needle 506; the first mounting seat 501 includes a first lower plate surface, a first supporting plate, and a first upper plate surface; the first lower plate surface is fixedly arranged on the base 1; the first upper plate surface is arranged right above the first lower plate surface; the first supporting plate is used for connecting the first upper plate surface and the first lower plate surface; the second stepping motor 503 is fixedly arranged at the bottom of the first upper plate surface, and a driving shaft of the second stepping motor penetrates through the first upper plate surface; the first rotating rod 504 is rotatably arranged through the first upper plate surface; the driving shaft of the second stepping motor 503 is drivingly connected to the first rotating lever 504 via the second driving belt 22; a third stepping motor 23 is arranged at the bottom of the first lower plate surface; a driving shaft of a third step motor 23 penetrates through the first supporting plate and the tail end of the driving shaft is provided with a first driving wheel 24; a first driven wheel 25 is arranged right above the first driving wheel 24; the first driving wheel 24 and the first driven wheel 25 are in transmission connection through a third transmission belt 26; the second bearing is sleeved at the bottom end of the first rotating rod 504; the outer ring of the second bearing is fixedly provided with a first transmission plate 28; the first driving plate 28 is fixedly connected with one side of the third driving belt 26; one end of the first rotating arm 505 is fixedly arranged at the top end of the first rotating rod 504; the vertical section of the "L-shaped" reagent needle 506 is arranged through the other end of the first rotating arm 505; a reagent tube 27 penetrates through the first rotating rod 504; the reagent tube 27 is connected at one end to the transverse section of an "L-shaped" reagent needle 506 and at the other end to the plunger pump 502. The second stepping motor 503 and the third stepping motor 23 receive instructions from the driving module 33, and the second stepping motor 503 realizes synchronous movement of the first rotating rod 504 and the driving shaft through the second transmission belt 22; the lifting of the first rotating rod 504 is achieved by the third stepping motor 23: in the third step, the relative positions of the first driving wheel 24 and the first driven wheel 25 on the driving shaft of the motor 23 are unchanged, and under the action of friction force, the third transmission belt 26 drives the first transmission plate 28 arranged on the third transmission belt to move up and down, so that the second bearing and the first rotating rod 504 arranged on the second bearing are driven to lift; the reagent needle 506 is used in cooperation with the reagent tube 17, and the plunger pump 502 sucks the reagent in the reagent bottle and retains the reagent at the front end of the reagent tube 17, so that the reagent can be conveniently injected into each cuvette subsequently.
In this embodiment, the sample adding and sampling device 4 preferably includes a second mounting seat 401, a peristaltic pump 402, a fourth stepping motor 403, a second rotating rod 404, a second rotating arm 405, and an "L-shaped" sample adding and sampling needle 406; the second mounting seat 401 includes a second lower plate surface, a second support plate, and a second upper plate surface; the second lower plate surface is fixedly arranged on the base 1; the second upper plate surface is arranged right above the second lower plate surface; the second supporting plate is used for connecting the second upper plate surface and the second lower plate surface; the fourth stepping motor 403 is fixedly arranged at the bottom of the second upper plate surface, and a driving shaft of the fourth stepping motor is arranged to penetrate through the second upper plate surface; the second rotating rod 404 is rotatably arranged through the second upper plate surface; a driving shaft of the fourth stepping motor 403 and the second rotating rod 404 are in transmission connection through a fourth transmission belt; a fifth stepping motor is arranged at the bottom of the second lower board surface; a driving shaft of the fifth stepping motor penetrates through the second supporting plate and a second driving wheel is arranged at the tail end of the driving shaft; a second driven wheel is arranged right above the second driving wheel; the second driving wheel and the second driven wheel are in transmission connection through a fifth transmission belt; a third bearing is sleeved at the bottom end of the second rotating rod 404; the outer ring of the third bearing is fixedly provided with a second transmission plate; the second transmission plate is fixedly connected with one side of the fifth transmission belt; one end of the second rotating arm 405 is fixedly arranged at the top end of the second rotating rod 404; the vertical section of the L-shaped sample adding and sampling needle 406 penetrates through the other end of the second rotating arm 405; a sample liquid tube 29 is arranged in the second rotating rod 404; sample tube 29 is connected at one end to the transverse section of an "L-shaped" sample application and sampling needle 406 and at the other end to peristaltic pump 402. The fourth stepping motor and the fifth stepping motor receive instructions from the driving module 33, and the fourth stepping motor realizes synchronous movement of the driving shaft and the first rotating rod 404 through a fourth transmission belt; the lifting of the first rotating rod 404 is realized by a fifth stepping motor: the relative positions of a second driving wheel and a second driven wheel on a driving shaft of the fifth stepping motor are unchanged, and under the action of friction force, a fifth transmission belt drives a second transmission plate arranged on the fifth driving belt to move up and down, so that a third bearing and a first rotating rod 404 arranged on the third bearing are driven to lift; application of sample sampling needle 406 cooperation sample liquid pipe 29 uses, and peristaltic pump 402 absorbs the appearance liquid in the sample cup to reserve this appearance liquid at sample liquid pipe 29 front end, be convenient for carry on follow-up this appearance liquid of pouring into in to corresponding cell.
In this embodiment, the weighing device 6 preferably includes a scale pan 601, a pressure sensor 602, and a screw motor 603; a screw rod of the screw rod motor 603 is arranged along the vertical direction; a slide block 30 is slidably arranged on a screw rod of the screw rod motor 603; the upper end of the slide block 30 is connected with a pressure sensor 602, and the upper end of the pressure sensor 602 is connected with a scale pan 601; the fixed platform 11 is provided with a sample groove 31 through the plate surface; scale pan 601 passes through sample slot 31; the scale 601 is positioned directly below the sample well 13. In the process of the spectrophotometric experiment, a first sample cup containing a sample is weighed, the weight of the sample cup accords with a set range, and the tray 304 rotates to weigh the next sample cup containing the sample.
In this embodiment, the cleaning device 7 preferably comprises a cleaning tank 701 mounted on the fixed platform, a first diaphragm pump 702 and a second diaphragm pump 703 mounted on the base, and a cleaning device 7; the first diaphragm pump 702 is provided with a waste pipe 34 and a drain pipe 36; the waste liquid pipe 34 connects the cleaning tank 701 and the first diaphragm pump 702; one end of the drain pipe 36 is connected with the first diaphragm pump 702, and the other end is connected with the waste liquid pool 40;
the second diaphragm pump 703 is provided with a water inlet pipe 38 and a water outlet pipe 39; one end of the water inlet pipe 38 is connected with the clean water tank 41, the other end is connected with the second diaphragm pump 703, and the water outlet pipe 39 is connected with the second diaphragm pump 703 and the cleaning tank 701; the water outlet pipe 39 is connected with a branch pipe 37; the branch pipe 37 is provided with an electromagnetic valve 35; the manifold 37 is terminated to a plunger pump 502.
The second diaphragm pump 703 sucks the clear water from the clear water tank 41 into the clear water tank 701, the first diaphragm pump 702 pumps the polluted clear water into the waste liquid tank 40, the clear water in the cleaning tank 701 is replaced immediately, and the pollution degree of the clear water can be reduced to the minimum; the first diaphragm pump 702 and the second diaphragm pump 703 are started simultaneously, the total operation time of the first diaphragm pump 702 is greater than or equal to that of the second diaphragm pump 703, and the overflow of clear water in the cleaning tank 701 is prevented; the outer wall of the reagent needle 506 only needs to extend into the cleaning pool 701 for cleaning, and the cleaning mode of the inner wall is as follows: the electromagnetic valve 37 is opened, clean water is introduced into the branch pipe 37, and the plunger pump 502 pumps the clean water in the branch pipe 37 into the reagent needle 506 and discharges the clean water into the cleaning tank 701, so that the inner wall of the reagent needle 506 is cleaned.
In this embodiment, preferably, the control system 9 includes a control motherboard 901 and a data switch 902 connected to the control motherboard 901; the connection mode of the control terminal 8 and the control main board 901 is wireless connection or electric connection; the data exchanger 902 comprises a temperature control module 32 and a driving module 33; the temperature control module 32 is connected with a temperature sensor and is used for controlling the temperature of the heating plate 20; the first driving module 33 is configured to control operations of the first stepping motor 301, the second stepping motor 503, the third stepping motor 23, the fourth stepping motor 403, the fifth stepping motor, the plunger pump 502, the peristaltic pump 402, the diaphragm pump 702, and the lead screw motor 603, so as to control the operations of the transmission blending device 3, the sample adding and sampling device 4, the reagent adding device 5, the weighing device 6, and the cleaning device 7.
The working steps of the automatic sample adding and mixing device for the pretreatment of the spectrophotometry, which is related by the embodiment, are as follows:
1. the scale plate rises to the highest point, a sample is placed into a sample cup, then the sample cup is placed into a sample hole 13 marked with a first position above the scale plate 601, the sample cup falls on the scale plate 601, the sample cup in the first sample hole 13 is marked as the first sample cup, the first sample cup with the sample is weighed, the weight of the sample cup accords with a set range, and the tray 304 rotates to weigh the next sample cup with the sample; when the weight of the sample cup is not in the set range, the tray 304 stops rotating; after the weights of all the sample cups are obtained, the scale plate 601 descends to an initial position, and the tray 304 rotates the first sample cup to a position to be added with liquid; the sample adding and sampling needle 406 rotates to the position of the cleaning pool 701 for cleaning, and rises to stay above the cleaning pool 701 after the cleaning is finished;
2. the second rotating rod rotates to drive the sample adding and sampling needle 406 to move to the position right above the sample cups, buffer solution is added to all the sample cups in sequence, the adding amount of the buffer solution is added according to the actual weight of the sample in each sample cup, namely, the buffer solution is added in proportion, all the buffer solution is added, and the sample adding and sampling needle 406 stands by above the tray 304; the tray 304 rotates clockwise and anticlockwise alternately to fully and uniformly mix the sample in the sample cup with the buffer solution; the sample adding and sampling needle 406 moves to the upper part of the first sample cup, descends to a certain height, and absorbs the sample liquid in the first sample cup; then the sample adding and sampling needle 406 rises and rotates to the upper part of the first cuvette, and the absorbed sample liquid is injected into the first cuvette; the sample adding and sampling needle 406 moves to a cleaning pool 701 for cleaning; respectively injecting the sample liquid in each sample cup into each cuvette until the sample liquid in all the sample cups is extracted; the tray 304 is reset to the initial position; the sample adding and sampling needle 406 moves to the cleaning pool 701 for cleaning, and after cleaning is completed, the sample adding and sampling needle 406 stands by above the cleaning pool 701;
3. cleaning the reagent needle 506, wherein the reagent needle 506 stands by above the cleaning pool 701 after the cleaning is finished; placing reagent bottles filled with different reagents in the reagent holes 14 respectively; the first rotating rod drives the reagent needle 506 to move to the upper part of one of the reagent bottles, so as to suck a certain amount of reagent in the reagent bottle, and the reagent required at the time is sucked into the reagent tube 27 at one time; the reagent needle 506 moves to the upper part of the first cuvette, the reagent is injected into the first cuvette, the position of the reagent needle 506 is kept still, the tray 304 is rotated, the reagent is sequentially injected into each cuvette in a one-suction and multi-strike mode, after all reagents are added, the reagent needle 506 rotates to the cleaning pool 701 for cleaning, after the cleaning is finished, the first rotating rod drives the reagent needle 506 to move, the second reagents are absorbed and injected into the cuvettes one by one, after all the second reagents are added, the reagent needle 506 rotates to the cleaning pool 701 for cleaning, and by analogy, the reagents in the remaining reagent bottles are injected into the cuvettes one by one; after each reagent is added to the tray 304, whether the tray 304 rotates and shakes up alternately clockwise and anticlockwise can be specified according to specific experimental requirements.
The above-described embodiments are merely illustrative of the principles and utilities of the present patent application and are not intended to limit the present patent application. Modifications and variations can be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of this patent application. Accordingly, it is intended that all equivalent modifications or changes which can be made by those skilled in the art without departing from the spirit and technical concepts disclosed in the present application shall be covered by the claims of this patent application.
Claims (9)
1. The utility model provides a preceding automatic application of sample mixing device of using of spectrophotometry, its characterized in that includes: the device comprises a base, a power supply, a transmission blending device, a sample adding and sampling device, a reagent adding device, a weighing device, a cleaning device, a control terminal and a control system, wherein the power supply is placed on the base;
the transmission blending device is fixedly arranged on the base through a support column; the sample adding and sampling device, the reagent adding device, the weighing device and the cleaning device are arranged around the transmission blending device; the control system is used for controlling the transmission blending device, the sample adding and sampling device, the reagent adding device, the weighing device and the cleaning device, and is also used for receiving an instruction sent by the control terminal; the control terminal is in the form of running control software in a network computer or a mobile phone.
2. The automatic sample-adding and mixing device for the pretreatment of the spectrophotometry as claimed in claim 1, wherein: the transmission blending device comprises a first stepping motor, a first transmission belt, a rotating shaft, a tray and a tray bracket; the tray support comprises the supporting column and a fixed platform; the supporting column is vertically arranged on the base; the fixed platform is horizontally and fixedly arranged at the top end of the supporting column;
the center of the fixed platform is provided with a shaft hole; the rotating shaft penetrates through the shaft hole; a first bearing is arranged in the shaft hole; the outer ring of the first bearing is embedded in the inner wall of the shaft hole; the inner ring of the first bearing is embedded in the surface of the rotating shaft; the tray is arranged in parallel to the fixed platform; the center of the tray is fixedly arranged at the top end of the rotating shaft; first step motor passes through "L type" mount and installs fixed platform's below, the vertical setting of its drive shaft, this drive shaft pass through first drive belt with the bottom transmission of pivot is connected.
3. The automatic sample-adding and mixing device for the pretreatment of the spectrophotometry as claimed in claim 2, wherein: the tray is disc-shaped; a plurality of sample holes, a plurality of reagent holes and a plurality of cuvette holes are arranged on the surface of the disc-shaped tray in a penetrating way; the sample holes, the reagent holes and the cuvette holes are uniformly distributed around the axis of the center of the disc-shaped tray respectively; the distance from the center of the cuvette hole to the axis is a, the distance from the center of the sample hole to the axis is b, and the distance from the center of the reagent hole to the axis is c; a ≦ b ≦ c;
a first connecting column is arranged on the lower bottom surface at the edge of the tray; the first connecting column is arranged perpendicular to the tray; the bottom end of the first connecting column is connected with an arc-shaped supporting plate; the arc-shaped supporting plate is arranged between the tray and the fixed platform, and the arc-shaped supporting plate is arranged right below all the reagent holes;
the bottom of the tray is also provided with a second connecting column; the bottom end of the second connecting column is connected with an annular supporting plate; the annular supporting plate is arranged between the tray and the fixed platform, and the annular supporting plate is arranged under all the cuvette holes.
4. The automatic sample-adding and mixing device for the pretreatment of the spectrophotometry as claimed in claim 3, wherein: the fixed platform is provided with a heating plate; a temperature sensor is arranged on the heating plate; a heating coil for heating is arranged in the heating plate; the heating plate is fixedly arranged at the center of the fixed platform; the center of the heating plate is provided with a through hole for the rotating shaft to pass through; the heating plate is provided with an annular groove for heating the cuvette; the annular supporting plate is arranged in the annular groove.
5. The automatic sample-adding and mixing device for the pretreatment of the spectrophotometry as claimed in claim 4, wherein: the reagent adding device comprises a first mounting seat, a plunger pump, a second stepping motor, a first rotating rod, a first rotating arm and an L-shaped reagent needle; the first mounting seat comprises a first lower plate surface, a first supporting plate and a first upper plate surface; the first lower plate surface is fixedly arranged on the base; the first upper plate surface is arranged right above the first lower plate surface; the first supporting plate is used for connecting the first upper plate surface and the first lower plate surface;
the second stepping motor is fixedly arranged at the bottom of the first upper plate surface, and a driving shaft of the second stepping motor penetrates through the first upper plate surface; the first rotating rod can rotatably penetrate through the first upper plate surface; the driving shaft of the second stepping motor is in transmission connection with the first rotating rod through a second transmission belt;
a third stepping motor is arranged at the bottom of the first lower plate surface; a driving shaft of the third stepping motor penetrates through the first supporting plate and a first driving wheel is arranged at the tail end of the driving shaft; a first driven wheel is arranged right above the first driving wheel; the first driving wheel and the first driven wheel are in transmission connection through a third transmission belt;
the second bearing is sleeved at the bottom end of the first rotating rod; the outer ring of the second bearing is fixedly provided with a first transmission plate; the first transmission plate is fixedly connected with one side of the third transmission belt;
one end of the first rotating arm is fixedly arranged at the top end of the first rotating rod; the vertical section of the L-shaped reagent needle penetrates through the other end of the first rotating arm; a reagent tube penetrates through the first rotating rod; one end of the reagent tube is connected with the L-shaped transverse section of the reagent needle, and the other end of the reagent tube is connected to the plunger pump.
6. The automatic sample-adding and mixing device for the pretreatment of the spectrophotometry as claimed in claim 5, wherein: the sample adding and sampling device comprises a second mounting seat, a peristaltic pump, a fourth stepping motor, a second rotating rod, a second rotating arm and an L-shaped sample adding and sampling needle; the second mounting seat comprises a second lower plate surface, a second supporting plate and a second upper plate surface; the second lower plate surface is fixedly arranged on the base; the second upper plate surface is arranged right above the second lower plate surface; the second supporting plate is used for connecting the second upper plate surface and the second lower plate surface;
the fourth stepping motor is fixedly arranged at the bottom of the second upper plate surface, and a driving shaft of the fourth stepping motor penetrates through the second upper plate surface; the second rotating rod can rotatably penetrate through the second upper plate surface; a driving shaft of the fourth stepping motor is in transmission connection with the second rotating rod through a fourth transmission belt;
a fifth stepping motor is arranged at the bottom of the second lower board surface; a driving shaft of the fifth stepping motor penetrates through the second supporting plate and a second driving wheel is arranged at the tail end of the driving shaft; a second driven wheel is arranged right above the second driving wheel; the second driving wheel and the second driven wheel are in transmission connection through a fifth transmission belt;
the bottom end of the second rotating rod is sleeved with a third bearing; the outer ring of the third bearing is fixedly provided with a second transmission plate; the second transmission plate is fixedly connected with one side of the fifth transmission belt;
one end of the second rotating arm is fixedly arranged at the top end of the second rotating rod; the vertical section of the L-shaped sample adding and sampling needle penetrates through the other end of the second rotating arm; a sample liquid pipe is arranged in the second rotating rod in a penetrating way; one end of the sample liquid pipe is connected with the L-shaped transverse section of the sample adding and sampling needle, and the other end of the sample liquid pipe is connected to the peristaltic pump.
7. The weighing device comprises a scale pan, a pressure sensor and a screw rod motor; a screw rod of the screw rod motor is arranged along the vertical direction; a sliding block is arranged on a screw rod of the screw rod motor in a sliding way; the upper end of the sliding block is connected with a pressure sensor, and the upper end of the pressure sensor is connected with a scale pan;
the fixed platform is provided with a sample groove through the plate surface; the scale pan penetrates through the sample groove; the scale plate is arranged right below the sample hole.
8. The automatic sample-adding and mixing device for the pretreatment of the spectrophotometry as claimed in claim 7, wherein: the automatic sample-adding and mixing device for the pretreatment of the spectrophotometry as claimed in claim, wherein: the cleaning device comprises a cleaning pool arranged on the fixed platform, a first diaphragm pump and a second diaphragm pump arranged on the base; the first diaphragm pump is provided with a waste liquid pipe and a liquid discharge pipe; the waste liquid pipe is connected with the cleaning pool and the first diaphragm pump; one end of the liquid discharge pipe is connected with the first diaphragm pump, and the other end of the liquid discharge pipe is connected with the waste liquid pool;
the second diaphragm pump is provided with a water inlet pipe and a water outlet pipe; one end of the water inlet pipe is connected with the clean water tank, the other end of the water inlet pipe is connected with the second diaphragm pump, and the water outlet pipe is connected with the second diaphragm pump and the cleaning tank; the water outlet pipe is connected with a branch pipe; the branch pipe is provided with an electromagnetic valve; the tail end of the branch pipe is connected to the plunger pump;
a placing table provided with a placing hole is fixedly arranged on the side wall of the fixed platform; the cleaning pool is placed in the placing hole.
9. The automatic sample-adding and mixing device for the pretreatment of the spectrophotometry as claimed in claim 8, wherein: the control system comprises a control mainboard and a data exchanger connected with the control mainboard; the control terminal is connected with the control main board in a wireless or electric connection mode; the data exchanger comprises a temperature control module and a driving module;
the temperature control module is connected with the temperature sensor and is used for controlling the temperature of the heating plate; the first driving module is used for controlling the operation of the first stepping motor, the second stepping motor, the third stepping motor, the fourth stepping motor, the fifth stepping motor, the plunger pump, the peristaltic pump, the diaphragm pump and the screw rod motor.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111121101.0A CN113866444A (en) | 2021-09-24 | 2021-09-24 | Automatic sample adding and mixing device for pretreatment of spectrophotometry |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111121101.0A CN113866444A (en) | 2021-09-24 | 2021-09-24 | Automatic sample adding and mixing device for pretreatment of spectrophotometry |
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| Publication Number | Publication Date |
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| CN113866444A true CN113866444A (en) | 2021-12-31 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202111121101.0A Pending CN113866444A (en) | 2021-09-24 | 2021-09-24 | Automatic sample adding and mixing device for pretreatment of spectrophotometry |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115611965A (en) * | 2022-09-23 | 2023-01-17 | 江苏铭晟聚太生物科技有限公司 | Multi-channel polypeptide synthesizer and synthesis method thereof |
-
2021
- 2021-09-24 CN CN202111121101.0A patent/CN113866444A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115611965A (en) * | 2022-09-23 | 2023-01-17 | 江苏铭晟聚太生物科技有限公司 | Multi-channel polypeptide synthesizer and synthesis method thereof |
| CN115611965B (en) * | 2022-09-23 | 2024-02-09 | 江苏明生聚太生物科技有限公司 | Multichannel polypeptide synthesizer and synthesizing method thereof |
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