CN115583856A - Compound growth regulating nutrient solution for improving quality of frangrant ainsliaea herb and application thereof - Google Patents
Compound growth regulating nutrient solution for improving quality of frangrant ainsliaea herb and application thereof Download PDFInfo
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
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- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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- A01N45/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds having three or more carbocyclic rings condensed among themselves, at least one ring not being a six-membered ring
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- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
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- C05G5/00—Fertilisers characterised by their form
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Abstract
The invention discloses a compound growth regulating nutrient solution for improving the quality of frangrant ainsliaea herb, which comprises the following components: the nutrient solution A consists of a water-soluble compound fertilizer, naphthylacetic acid and 6-benzylaminopurine, the nutrient solution B consists of a water-soluble compound fertilizer, triiodobenzoic acid, gibberellin and a nutrient solution C consists of a water-soluble compound fertilizer, triiodobenzoic acid and gibberellin, and the nutrient solution D consists of copper sulfate, abscisic acid, phenylalanine and brassinolide; compared with the prior art, the method improves the highest yield by 1.07 times when the Ainsliaea fragrans is fertilized and planted, improves the highest chlorogenic acid content by 1.11 times when the Ainsliaea fragrans is not fertilized and planted, and solves the contradiction that the yield and the yield are not high when the Ainsliaea fragrans is planted.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicinal materials and agriculture, in particular to a compound growth regulating nutrient solution for improving the quality of Ainsliaea fragrans champ and application thereof.
Background
Ainsliaea fragrans Champ (Ainsliaea fragrans Champ) is a perennial herb of Ainsliaea fragrans of Compositae, is used as a medicine by using whole herbs, has the effects of clearing heat and removing toxicity, promoting diuresis and stopping bleeding, and is mainly used for treating pulmonary abscess, mastitis, malnutritional stagnation, osteomyelitis and venomous snake bite.
The frangrant ainsliaea herb contains chlorogenic acid, apigenin, quercetin, luteolin, 3, 5-dicaffeoylquinic acid and the like, wherein the content of the chlorogenic acid is taken as a core index of the quality of the frangrant ainsliaea herb according to the internal inspection medicinal material standard of general pharmaceutical enterprises, and meanwhile, the higher the content is, the better the clinical efficacy is.
At present, the main source of the Ainsliaea fragrans medicinal material is wild, the medicinal material is collected from all over the country, and the medicinal material has different quality, so that the medicinal effect of the traditional Chinese medicine preparation taking the Ainsliaea fragrans medicinal material as the raw material is directly influenced. In recent years, wild introduction and cultivation tests of Ainsliaea fragrans have been gradually developed in China, but at present, only simple experimental researches on planting technologies have been made by Jiangxianmei and the like (Chinese medicine Ainsliaea fragrans ecological characteristics and domestication and cultivation [ J ], jiangxi forestry science and technology, 2006 (4)).
When the Xingxiang Tuerfeng is originally introduced to be cultivated under a photovoltaic panel, the team of the invention discovers that the growth cycle of the Xingxiang Tuerfeng is short, so the contradiction between the key effective component chlorogenic acid and the yield thereof is caused, namely, the high yield can be obtained by applying fertilizer, but the content of the chlorogenic acid cannot reach the standard, so the chlorogenic acid cannot be purchased by medicine enterprises, but the content of the chlorogenic acid cannot reach the standard by applying no fertilizer, but the yield is sharply reduced, and effective economic benefit cannot be obtained.
Disclosure of Invention
Aiming at the problems of the prior art and the Ainsliaea fragrans champ, the invention particularly provides a technology for applying a plant growth regulator and a chemical fertilizer to the Ainsliaea fragrans champ planting industry.
In order to achieve the purpose, the invention adopts the technical scheme that: the compound growth regulating nutrient solution for improving the quality of frangrant ainsliaea herb comprises a nutrient solution A, a nutrient solution B, a nutrient solution C and a nutrient solution D:
the nutrient solution A comprises the following components: 0.5-1.0 g/L of water-soluble compound fertilizer, 150-200 mg/L of naphthylacetic acid (NAA) and 120-150 mg/L of 6-benzylaminopurine (6-BA); the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20;
the nutrient solution B comprises the following components: 2.0-3.0 g/L of water-soluble compound fertilizer, 100-120 mg/L of triiodobenzoic acid (TIBA) and 100-150 mg/L of gibberellin (GA 3); the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20;
the nutrient solution C comprises the following components: 3.0-4.0 g/L of water-soluble compound fertilizer, 100-120 mg/L of triiodobenzoic acid (TIBA) and 60-80 mg/L of gibberellin; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20;
the nutrient solution D comprises the following components: 5-10 mg/L of copper sulfate, 5-8 mg/L of abscisic acid (ABA), 1.0-1.5 g/L of phenylalanine (Phe) and 0.1-0.2 mg/L of Brassinolide (BR).
The pH value of the nutrient solution formula is adjusted to 5.8-6.2 when in use.
The specific use method of the compound growth regulating nutrient solution for improving the quality of frangrant ainsliaea herb comprises the following steps:
(1) After sowing for 4-5 months, spraying the nutrient solution A on the leaf surface of 40-60 kg/mu when the seedlings are full; the nutrient solution A comprises the following components: 0.5-1.0 g/L of water-soluble compound fertilizer, 150-200 mg/L of naphthylacetic acid (NAA) and 120-150 mg/L of 6-benzylaminopurine (6-BA); the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20;
(2) Irrigating the roots once with the nutrient solution A at 200-250 kg/mu before the upright stems are drawn in 6-7 months, and irrigating the roots once with the nutrient solution A at 200-250 kg/mu every other 10 days; the nutrient solution A comprises the following components: 0.5-1.0 g/L of water-soluble compound fertilizer, 150-200 mg/L of naphthylacetic acid (NAA) and 120-150 mg/L of 6-benzylaminopurine (6-BA); the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20;
(3) When the full growth period of the nutrition is 8 months, irrigating the roots once by 200-250 kg/mu of nutrient solution B, and irrigating the roots once by 200-250 kg/mu of nutrient solution B every other 10 days; the nutrient solution B comprises the following components: 2.0-3.0 g/L of water-soluble compound fertilizer, 100-120 mg/L of triiodobenzoic acid (TIBA) and 100-150 mg/L of gibberellin (GA 3); the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20;
(4) When flower buds begin to form in 9 months, irrigating the roots once with the nutrient solution C according to 200-250 kg/mu, and irrigating the roots once with the nutrient solution C according to 200-250 kg/mu every other 15 days; the nutrient solution C comprises the following components: 3.0-4.0 g/L of water-soluble compound fertilizer, 100-120 mg/L of triiodobenzoic acid (TIBA) and 60-80 mg/L of gibberellin; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20;
(5) Spraying the nutrient solution D on the leaf surface once per 40-60 kg/mu in the full-bloom stage of 10 months, and spraying the nutrient solution D on the leaf surface once per 40-60 kg/mu every 7 days later; the nutrient solution D comprises the following components: 5-10 mg/L of copper sulfate, 5-8 mg/L of abscisic acid (ABA), 1.0-1.5 g/L of phenylalanine (Phe) and 0.1-0.2 mg/L of Brassinolide (BR).
The beneficial technical effects of the invention are as follows:
1. the invention applies two plant growth regulators of naphthylacetic acid and 6-benzylaminopurine with certain concentration respectively after seedlings come out completely in 4-5 months and before upright stems begin to be extracted in 6-7 months, and is matched with high-nitrogen fertilizer, so that the invention can effectively promote the clustered buds of the base part to emit more leaves, simultaneously widen the leaves, effectively delay the time of extracting the upright stems and increase the vegetative growth time of the base part.
2. The invention applies triiodobenzoic acid and gibberellin with certain concentration in the full growth period of 8 months and the early period of 9 months of flowers, and simultaneously cooperates with a high nitrogen-potassium compound fertilizer, so as to effectively promote the upward growth of the upright stem and the emergence of the lateral branches.
3. The accumulation of the chlorogenic acid of the Ainsliaea fragrans champ is mainly in the reproductive growth period, and in the full-bloom period of 10 months, the two plant growth regulators of abscisic acid and brassinolide with certain concentrations are used and are compounded with copper sulfate and phenylalanine with certain concentrations, so that the rapid accumulation of the chlorogenic acid in the Ainsliaea fragrans champ can be effectively promoted.
4. Compared with the prior art, the method improves the highest yield by 1.07 times when the Ainsliaea fragrans is fertilized and planted, improves the highest chlorogenic acid content by 1.11 times when the Ainsliaea fragrans is not fertilized and planted, and solves the contradiction that the yield and the yield are not high when the Ainsliaea fragrans is planted.
5. The naphthylacetic acid, 6-benzylaminopurine, triiodobenzoic acid, gibberellin, brassinolide and abscisic acid applied by the invention are plant growth regulators, belong to chemical reagents which are basically non-toxic to human bodies, and meanwhile, the dosage of the invention is extremely low, so that the damage to the environment is avoided, and the phenylalanine in the invention is an amino acid and cannot cause harm to the human bodies and the environment.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1: example 1 effectively promoted increase in basal leaf shroud width;
FIG. 2: example 1 effectively promotes the height of "bolting";
FIG. 3: example 1 effectively promotes the growth of the tiller bud in "bolting";
FIG. 4: the germination rate of the seeds harvested in example 1 was not affected.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without inventive step based on the embodiments of the present invention, are within the scope of protection of the present invention.
A large number of researches show that fertilization can promote the accumulation of the biomass of the whole plant, namely the accumulation of primary metabolites to different degrees. In most cases, the accumulation of the primary metabolite is beneficial to the accumulation of the secondary metabolite, but under artificial conditions, the accumulation speed of the secondary metabolite cannot catch up with the accumulation speed of the primary metabolite, so that the biomass of the secondary metabolite in a plant is increased, but the content of the secondary metabolite is rapidly reduced.
The plant secondary metabolite does not directly participate in the growth and development process of the plant, but plays an important role in the aspects of improving the adaptability and interspecific competitive power of the plant to the physical environment, resisting the attack of natural enemies, enhancing the disease resistance and the like, and the active ingredients of medicinal plants are mostly derived from the secondary metabolite, namely chlorogenic acid in the invention, namely the secondary metabolite belonging to the Ainsliaea fragrans champ.
The generation of secondary metabolites of plants in nature is influenced by environmental factors, such as drought, water accumulation, saline and alkaline, heavy metals, illumination intensity, illumination duration and the like, while different plants, different organs of the same plant and different secondary metabolites may produce different results under different environmental factors. In the process of planting medicinal plants, elicitors are often adopted to induce the generation of plant secondary metabolites, and different elicitors, induction concentrations, action times, action parts and the like have specificity on different plants, different organs and different target substances, and are supposed to be related to the expression regulation and control of related enzyme genes. Currently, elicitors are mainly classified into microbial elicitors such as fungal spores, mycelia, homogenate, fungal culture solution and the like, abiotic elicitors such as physical elicitors such as temperature, drought, rays and the like, chemical elicitors such as heavy metal ions of copper, cadmium, zinc, iron, silver and the like, amino acids and the like, plant growth regulators such as ABA, meJA, SA, GA3, BR, CCC and the like. Different elicitors may also have interaction, may produce synergistic or antagonistic effects, or may not interact with each other.
In the planting process of Ainsliaea fragrans champ, economic benefits cannot be formed without fertilization, but the output-input ratio is smaller than 1, but the quality of medicinal materials cannot reach the standard due to fertilization, and the economic benefits cannot be realized. In the beginning of the invention, the organic fertilizer and the compound fertilizer are applied in combination with the medium trace elements for fertilization, although the content of the chlorogenic acid is improved to a certain extent, the content of the chlorogenic acid in the medicinal materials is still below the qualified line.
And a large number of experiments are carried out by adopting the elicitor, so that the yield of the medicinal materials is increased, and the quality of the medicinal materials can reach the standard. Part of the test procedure is recorded as follows:
1. screening for elicitor sensitivity and preliminary concentration
In the initial growth stage of 9 months (when flower buds begin to form), plant growth regulators with different concentrations are used as elicitors for root irrigation, and the elicitors with the same concentration and the same type are used for root irrigation again after 10 days, and the specific experimental design is shown in table 1:
table 1: design of inducer sensitivity test
Collected in the next 2 months, the content of chlorogenic acid in the whole grass is determined, and the result is shown in table 2:
table 2: results of inducer sensitivity test
As can be seen from the table 2, 50-100 mg/L of TIBA, 0.1mg/L of BR, 5-20 mg/L of ABA, 50-100 mg/L of LGA3 and the like have obvious promotion effects on the accumulation of chlorogenic acid of the Ainsliaea fragrans champ, particularly 5mg/L of ABA has the most obvious promotion effect, and in the test process, the application of ABA has the phenomenon of promoting the premature senility of the Ainsliaea fragrans champ to a certain extent, can promote all the seeds to be harvested in 1 month from 12 months to the next year, and simultaneously has certain effects on the seed setting rate and the seed germination rate, the concentration is higher, the seed setting rate and the seed germination rate are greatly influenced, and the biological quantity accumulation is greatly influenced.
GA3 and BR with proper concentrations can promote the accumulation of chlorogenic acid and slightly improve the yield.
However, NAA and 6-BA can not promote the accumulation of chlorogenic acid, but can promote the tillering of axillary buds and can greatly promote the accumulation of biomass, thereby increasing the yield, which also can be the reason for the reduction of the content of chlorogenic acid after the application.
2. Elicitor combination test
In the initial stage of 9-month reproductive growth (when flower buds begin to form), the root irrigation is carried out by compounding sensitive elicitors with proper concentration, and the root irrigation is carried out again by using the elicitors with the same concentration and the same type after 10 days, wherein the specific experimental design is shown in a table 3:
table 3: design of inducer orthogonal experiment
Collected in 2 months of the next year, and the content of chlorogenic acid in the whole herb is determined, and the result is shown in table 4:
table 4: results of inducer orthogonal test
As can be seen from Table 4, the combination of the elicitors can obviously improve the content of chlorogenic acid, the improvement degree of the combination with ABA on the content of chlorogenic acid is larger than that of the combination without ABA, which indicates that the ABA is most effective for the accumulation of chlorogenic acid, and the improvement degrees of FP5 and FP9 on the content of chlorogenic acid are obviously higher than that of other combinations with ABA, which indicates that ABA and GA3 have certain antagonistic action on the accumulation of chlorogenic acid.
In the test process, the biomass of individual plants of FP6 and FP8 is close to that of FP1, while the biomass of the combined individual plants with ABA is lower than that of FP1, wherein FP4 and FP7 are the most obvious, which shows that BR and ABA have antagonistic effect on the accumulation of Pleurotus eryngii.
3. Plant growth regulator yield test
In the first full growth period of 6 months, plant growth regulators with different concentrations and types are selected to irrigate roots, and the roots are irrigated once after every 10 days, and the specific experimental design is shown in the following table 5:
table 5: orthogonal experimental table for influence of plant growth regulator on yield
After 60 days of the test, the plants are dug and the biological growth amount per mu is measured.
Table 6: test results of the Effect of plant growth regulators on yield
As can be seen from Table 6, the combination of NAA and 6-BA, with or without BR or GA3, can significantly increase the biomass of Ainsliaea fragrans, while the combination of NAA and 6-BA, with the addition of BR or GA3, can only slightly increase the extent of increasing the biomass accumulation, but not significantly. Meanwhile, it was found during the experiments that when NAA was combined with GA3, the plants became particularly delicate and prone to lodging, whether or not BR or 6-BA was present.
Example 1
1. After sowing in the beginning of 4 months, when seedlings are full in the middle ten days of 4 months, spraying a nutrient solution A consisting of 0.5g/L of water-soluble compound fertilizer (mass ratio of nitrogen, phosphorus and potassium is 20.
2. Before the uprighted stem extraction in the middle ten days of 6 months, the root is irrigated once by using a nutrient solution A consisting of 0.5g/L of water-soluble compound fertilizer (the mass ratio of nitrogen, phosphorus and potassium is 20.
3. After every 10 days, the roots are irrigated once by using nutrient solution A consisting of 0.5g/L of water-soluble compound fertilizer (mass ratio of nitrogen, phosphorus and potassium is 20: 5).
4. When the growth period is full of nutrition in the beginning of 8 months, irrigating roots once by using nutrient solution B consisting of 2.0g/L of water-soluble compound fertilizer (mass ratio of nitrogen, phosphorus and potassium is 20.
5. After every 10 days, the roots are irrigated once by using a nutrient solution B consisting of 2.0g/L of water-soluble compound fertilizer (the mass ratio of nitrogen, phosphorus and potassium is 20: 10), 100mg/L of triiodobenzoic acid (TIBA) and 100mg/L of gibberellin (GA 3), and the dosage per mu is 200kg.
6. When flower buds begin to form in the last ten days of 9 months, the roots are irrigated once by nutrient solution C consisting of 3.0g/L of water-soluble compound fertilizer (mass ratio of nitrogen, phosphorus and potassium is 20: 20).
7. After 15 days, the root is irrigated once by using nutrient solution C consisting of 3.0g/L of water-soluble compound fertilizer (the mass ratio of nitrogen, phosphorus and potassium is 20: 20), 100mg/L of triiodobenzoic acid (TIBA) and 60mg/L of gibberellin, and the dosage per mu is 200kg.
8. In the full bloom stage of 10 months, the nutrient solution D consisting of 5mg/L of copper sulfate, 5mg/L of abscisic acid (ABA), 1.0g/L of phenylalanine (Phe) and 0.1mg/L of Brassinolide (BR) is sprayed on the leaf surface once, and the dosage per mu is 50kg.
9. After every 7 days, nutrient solution D consisting of 5mg/L of copper sulfate, 5mg/L of abscisic acid (ABA), 1.0g/L of phenylalanine (Phe) and 0.1mg/L of Brassinolide (BR) is sprayed on the leaf surface once, and the dosage per mu is 50kg.
The pH of the nutrient solution formula is adjusted to 5.8 before use.
10. In the planting process, the growth process of lepriac is recorded by pictures, the result is shown in figures 1-3, the lepriac is collected in 1 month of the next year, the yield per mu, the content of chlorogenic acid and the germination rate thereof are measured (shown in figure 4), the content of the chlorogenic acid in the lepriac is measured according to the item of 'sample solution preparation' published by Zhongchang et al (the high performance liquid phase gradient elution method is used for measuring chlorogenic acid and rutin in lepriac [ J ], an analytical laboratory, 2008.
Example 2
1. After sowing in 4-month late ten days, when seedlings are aligned in 5-month last ten days, 1.0g/L of water-soluble compound fertilizer (mass ratio of nitrogen, phosphorus and potassium is 20.
2. Before the stem extraction in the late 6 th month, 1.0g/L of nutrient solution A consisting of water-soluble compound fertilizer (nitrogen, phosphorus and potassium mass ratio: 20: 5).
3. After every 10 days, the root is irrigated once by nutrient solution A consisting of 1.0g/L of water-soluble compound fertilizer (mass ratio of nitrogen, phosphorus and potassium is 20: 5) and 150mg/L of naphthylacetic acid (NAA), 6-benzylaminopurine (6-BA), and the dosage per mu is 250kg.
4. In the full growth period of the vegetative growth in the middle 8 th month, the root is irrigated once with a nutrient solution B consisting of 3.0g/L of water-soluble compound fertilizer (mass ratio of nitrogen, phosphorus and potassium: 20).
5. After every 10 days, the roots are irrigated once by using nutrient solution B consisting of 3.0g/L of water-soluble compound fertilizer (mass ratio of nitrogen, phosphorus and potassium is 20: 10), 120mg/L of triiodobenzoic acid (TIBA) and 150mg/L of gibberellin (GA 3), and the dosage per mu is 250kg.
6. When flower buds begin to form in the first ten days of 9 months, the roots are irrigated once with nutrient solution C consisting of 4.0g/L water-soluble compound fertilizer (mass ratio of nitrogen, phosphorus and potassium: 20), 120mg/L triiodobenzoic acid (TIBA) and 80mg/L gibberellin, and the dosage per mu is 250kg.
7. After 15 days, the root of the plant is irrigated once by using a nutrient solution C consisting of 4.0g/L of water-soluble compound fertilizer (mass ratio of nitrogen, phosphorus and potassium is 20: 20).
8. In the full bloom stage of 10 months, a nutrient solution D consisting of 5mg/L of copper sulfate, 8mg/L of abscisic acid (ABA), 1.5g/L of phenylalanine (Phe) and 0.2mg/L of Brassinolide (BR) is sprayed on the leaf surface once, and the dosage per mu is 60kg.
9. After every 7 days, a nutrient solution D consisting of 5mg/L of copper sulfate, 8mg/L of abscisic acid (ABA), 1.5g/L of phenylalanine (Phe) and 0.2mg/L of Brassinolide (BR) is sprayed on the leaf surface of the leaf once, and the dosage per mu is 60kg.
The four nutrient solutions A, B, C and D in the steps are all adjusted to be 6.2 before use.
10. Collected in 2 months of the next year, the yield per mu and the content of chlorogenic acid are measured, the content of chlorogenic acid in frangrant ainsliaea herb is measured according to the item of 'sample solution preparation' published by Zhoushou et al (the high performance liquid gradient elution method is used for measuring chlorogenic acid and rutin in frangrant ainsliaea herb [ J ], the analytical laboratory is used for 27 (5)), and the samples are measured according to the chromatographic conditions under the content measurement item under 'compound frangrant ainsliaea herb granules' in the part of '2020 edition of Chinese pharmacopoeia'.
Comparative example 1
1. Before sowing in the beginning of 4 months, combining rotary tillage, and broadcasting and applying 50kg of compound fertilizer (mass ratio of nitrogen, phosphorus and potassium is 15.
2. In the middle ten days of 4 months, after manual watering, urea is applied, and the amount of urea per mu is 5kg.
3. And (3) in the first 8 months of the full-growth period, spreading additional fertilizer by using 30kg of compound fertilizer (the mass ratio of nitrogen, phosphorus and potassium is 20.
4. And when flower buds begin to form in the middle ten days of 9 months, spreading the fertilizer with 30kg of compound fertilizer (the mass ratio of nitrogen, phosphorus and potassium is 20.
5. Collected in 1 month of the next year, the yield per mu and the content of chlorogenic acid are measured, the content of chlorogenic acid in frangrant ainsliaea herb is measured according to the item of 'sample solution preparation' published by Zhoushanqigong et al (the high performance liquid gradient elution method is used for measuring chlorogenic acid and rutin in frangrant ainsliaea herb [ J ], an analytical laboratory, 2008 (27 (5)), and the samples are measured according to the chromatographic conditions under the content measurement item under 'compound frangrant ainsliaea herb granules' in the first part of 'Chinese pharmacopoeia' 2020 edition.
Comparative example 2
1. Before sowing in the beginning of 4 months, combining rotary tillage, and broadcasting and applying 50kg of compound fertilizer (mass ratio of nitrogen, phosphorus and potassium is 15.
2. And then performing conventional management without additional fertilization.
3. Collected in 1 month of the next year, the yield per mu and the content of chlorogenic acid are measured, the content of chlorogenic acid in frangrant ainsliaea herb is measured according to the item of 'sample solution preparation' published by Zhoushanqigong et al (the high performance liquid gradient elution method is used for measuring chlorogenic acid and rutin in frangrant ainsliaea herb [ J ], an analytical laboratory, 2008 (27 (5)), and the samples are measured according to the chromatographic conditions under the content measurement item under 'compound frangrant ainsliaea herb granules' in the first part of 'Chinese pharmacopoeia' 2020 edition.
Comparative example 3
1. Sowing the seeds at the beginning of 4 months without applying base fertilizer.
2. In the middle ten days of 4 months, after manual watering, urea is applied, and the amount of urea per mu is 5kg.
3. And (3) in the first 8 months of the full-growth period, spreading additional fertilizer by using 30kg of compound fertilizer (the mass ratio of nitrogen, phosphorus and potassium is 20.
4. And (3) when flower buds begin to form in the middle ten days of 9 months, spreading the fertilizer once by using 30kg of compound fertilizer (mass ratio of nitrogen, phosphorus and potassium is 20.
5. Collected in 1 month of the next year, the per mu yield and the content of chlorogenic acid are measured, and the content of chlorogenic acid in frangrant ainsliaea is measured according to the item of 'sample solution preparation' published by Zhoushu et al (high performance liquid gradient elution method for measuring chlorogenic acid and rutin in frangrant ainsliaea [ J ], analytical laboratory, 2008 (27 (5)), and the samples are measured according to the chromatographic conditions under the content measurement item under 'compound frangrant ainsliaea granules' in the first part of 2020 edition of Chinese pharmacopoeia.
Comparative example 4
1. Sowing is carried out in the beginning of 4 months, no base fertilizer and additional fertilizer are carried out, and other management is conventional management.
2. Collected in 1 month of the next year, the per mu yield and the content of chlorogenic acid are measured, and the content of chlorogenic acid in frangrant ainsliaea is measured according to the item of 'sample solution preparation' published by Zhoushu et al (high performance liquid gradient elution method for measuring chlorogenic acid and rutin in frangrant ainsliaea [ J ], analytical laboratory, 2008 (27 (5)), and the samples are measured according to the chromatographic conditions under the content measurement item under 'compound frangrant ainsliaea granules' in the first part of 2020 edition of Chinese pharmacopoeia.
The acre yields, chlorogenic acid contents and germination rates of examples 1 and 2 were compared with those of comparative examples 1 to 4, and the results are shown in Table 7:
table 7: comparative results of examples and comparative examples
| Processing \ results | Yield per mu | Chlorogenic acid content | Germination rate of seeds |
| Example 1 | 138.6kg | 1.37% | 69.4% |
| Example 2 | 131.9kg | 1.34% | 68.9% |
| Comparative example 1 | 123.1kg | 0.67% | 64.9% |
| Comparative example 2 | 102.4kg | 0.91% | 51.3% |
| Comparative example 3 | 110.3kg | 0.76% | 54.8% |
| Comparative example 4 | 80.4kg | 1.21% | 39.6% |
As can be seen from the above table, the yield of the comparative example 1, which is applied with both the base fertilizer and the additional fertilizer, is 1.5 times that of the comparative example 4, but the chlorogenic acid content is only 0.67% which is 0.55 times that of the comparative example 4.
The yield per mu of the embodiment 1 and the embodiment 2 in the invention is 1.12 times and 1.07 times of that of the comparison example 1 in the optimal comparison example, and the content of chlorogenic acid is 1.13 times and 1.11 times of that of the comparison example 4 in the optimal comparison example, thereby perfectly solving the contradiction between yield and quality in the Ainsliaea fragrans planting process.
Finally, it should be noted that: although the present invention has been described in detail with reference to the above embodiments, it should be understood by those skilled in the art that: modifications and equivalents may be made thereto without departing from the spirit and scope of the invention and it is intended to cover in the claims the invention as defined in the appended claims.
Claims (3)
1. The compound growth regulating nutrient solution for improving the quality of frangrant ainsliaea herb comprises a nutrient solution A, a nutrient solution B, a nutrient solution C and a nutrient solution D, and is characterized in that:
the nutrient solution A comprises the following components: 0.5-1.0 g/L of water-soluble compound fertilizer, 150-200 mg/L of naphthylacetic acid and 120-150 mg/L of 6-benzylaminopurine; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20;
the nutrient solution B comprises the following components: 2.0-3.0 g/L of water-soluble compound fertilizer, 100-120 mg/L of triiodobenzoic acid and 100-150 mg/L of gibberellin; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20;
the nutrient solution C comprises the following components: 3.0-4.0 g/L of water-soluble compound fertilizer, 100-120 mg/L of triiodobenzoic acid and 60-80 mg/L of gibberellin; the mass ratio of nitrogen, phosphorus and potassium in the water-soluble compound fertilizer is 20;
the nutrient solution D comprises the following components: 5-10 mg/L of copper sulfate, 5-8 mg/L of abscisic acid, 1.0-1.5 g/L of phenylalanine and 0.1-0.2 mg/L of brassinolide.
2. The application of the compound growth-regulating nutrient solution for improving the quality of Ainsliaea fragrans champ of claim 1 is characterized in that the application method comprises the following steps:
(1) After sowing for 4-5 months, spraying the nutrient solution A on the leaf surface of 40-60 kg/mu when the seedlings are full;
(2) Before the upright stem is extracted in 6-7 months, irrigating the root with the nutrient solution A once according to 200-250 kg/mu, and irrigating the root with the nutrient solution A once again according to 200-250 kg/mu every other 10 days;
(3) When the full growth period of the nutrition is 8 months, irrigating the roots once by 200-250 kg/mu of nutrient solution B, and irrigating the roots once by 200-250 kg/mu of nutrient solution B every other 10 days;
(4) Irrigating roots with the nutrient solution C once every 9 months when flower buds begin to form, and irrigating roots with the nutrient solution C once every other 15 days, wherein the nutrient solution C once every other 15 days is 200-250 kg/mu;
(5) And spraying the nutrient solution D on the leaf surface once every 40-60 kg/mu in the 10-month full-bloom period, and spraying the nutrient solution D on the leaf surface once every other 7 days in a range of 40-60 kg/mu.
3. The application of the compound growth-regulating nutrient solution for improving the quality of frangrant ainsliaea herb according to claim 2, wherein the pH values of the nutrient solution A, the nutrient solution B, the nutrient solution C and the nutrient solution D are regulated to 5.8-6.2.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103524198A (en) * | 2012-07-06 | 2014-01-22 | 中国农业科学院棉花研究所 | Foliage fertilizer for promoting growth of cotton buds and bolls and preparation method thereof |
| CN104303871A (en) * | 2014-09-20 | 2015-01-28 | 云南省农业科学院经济作物研究所 | Remedying method of frostbitten potatoes at seedling stage |
| CN104756655A (en) * | 2015-03-30 | 2015-07-08 | 中国农业科学院北京畜牧兽医研究所 | Fertilization method for increasing yield of alfalfa |
| CN104920394A (en) * | 2015-05-26 | 2015-09-23 | 深圳市易普乐生物科技有限公司 | Composite nutrient solution for adjusting growth of crops, preparation method and application thereof |
| CN108243898A (en) * | 2018-02-08 | 2018-07-06 | 玉溪云星生物科技有限公司 | A kind of nutrient solution of African Chrysanthemum soilless culture substrate |
| CN112006023A (en) * | 2020-08-26 | 2020-12-01 | 广东碧然美景观艺术有限公司 | Medicament for regulating flowering phase of garlic and rattan and regulating method thereof |
| CN112616542A (en) * | 2020-12-16 | 2021-04-09 | 广西南宁碧湾园生态农业开发有限公司 | Flower control cultivation method for yellow sophora |
-
2022
- 2022-12-12 CN CN202211588857.0A patent/CN115583856B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103524198A (en) * | 2012-07-06 | 2014-01-22 | 中国农业科学院棉花研究所 | Foliage fertilizer for promoting growth of cotton buds and bolls and preparation method thereof |
| CN104303871A (en) * | 2014-09-20 | 2015-01-28 | 云南省农业科学院经济作物研究所 | Remedying method of frostbitten potatoes at seedling stage |
| CN104756655A (en) * | 2015-03-30 | 2015-07-08 | 中国农业科学院北京畜牧兽医研究所 | Fertilization method for increasing yield of alfalfa |
| CN104920394A (en) * | 2015-05-26 | 2015-09-23 | 深圳市易普乐生物科技有限公司 | Composite nutrient solution for adjusting growth of crops, preparation method and application thereof |
| CN108243898A (en) * | 2018-02-08 | 2018-07-06 | 玉溪云星生物科技有限公司 | A kind of nutrient solution of African Chrysanthemum soilless culture substrate |
| CN112006023A (en) * | 2020-08-26 | 2020-12-01 | 广东碧然美景观艺术有限公司 | Medicament for regulating flowering phase of garlic and rattan and regulating method thereof |
| CN112616542A (en) * | 2020-12-16 | 2021-04-09 | 广西南宁碧湾园生态农业开发有限公司 | Flower control cultivation method for yellow sophora |
Non-Patent Citations (1)
| Title |
|---|
| 郑帅明等: "豫西无公害烟草测土配方与营养套餐施肥技术", 《河南农业》 * |
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