CN115530173A

CN115530173A - Synergistic pesticide for preventing and treating sugarcane borers

Info

Publication number: CN115530173A
Application number: CN202211293275.XA
Authority: CN
Inventors: 邓宇驰; 宋修鹏
Original assignee: Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
Current assignee: Guangxi Zhuang Nationality Autonomous Region Academy of Agricultural Sciences
Priority date: 2021-08-31
Filing date: 2021-08-31
Publication date: 2022-12-30
Also published as: CN113826633B; CN113826633A

Abstract

The invention relates to the technical field of pesticides, and particularly relates to a synergistic pesticide for preventing and treating sugarcane borers. A synergistic insecticidal composition for preventing and treating sugarcane borer comprises the active ingredients of tianweisu and thiocyclam which are compounded in a binary way; wherein the mass ratio of the ivermectin to the thiocyclam is 1-20:30-1. The components of the invention are reasonable, and the activity after compounding is not the simple superposition of the activities of all the components, but has obvious synergistic effect. Compared with single pesticide, the pesticide can improve the insecticidal effect, can expand the insecticidal spectrum, and can effectively solve the problem of using single-component pesticide to prevent and control sugarcane borers. And the insecticidal mechanisms of the 2 active ingredients are different during compounding, so that the generation of drug resistance of pests can be delayed, and the pesticide has a certain protection effect on the pesticide.

Description

Synergistic pesticide for preventing and treating sugarcane borers

The application is that the patent number is: 2021110161014, patent name: the pesticide for preventing and treating the sugarcane borers is applied for the day as follows: 20210831, applicant (patentee): divisional application of the application of Guangxi Zhuang autonomous region academy of agricultural sciences.

Technical Field

The invention relates to the technical field of pesticides, and particularly relates to a synergistic pesticide for preventing and treating sugarcane borers.

Background

Sugarcane is the main economic crop of Guangxi, and sugarcane borer and sugarcane tortoise are two main pests in Guangxi sugarcane producing areas, wherein the harm of the sugarcane borer to the sugarcane is particularly prominent. The sugarcane borers are harmful in the whole growth period of the sugarcane, the growth points are harmful in the seedling period to cause dead seedlings, the dead rate is generally 10% -20%, the serious growth rate is more than 50%, the sugarcane stalks are damaged by boring at the middle and later periods, the tissue of the sugarcane stalks is damaged, the growth of the sugarcane is hindered, the wind break is caused, the yield and the sugar content are reduced, the sugarcane is damaged by various borers in the whole growth process, and the loss is about 0.25-0.5t every 0.067 ha. . Since 31 months and 10 months in 2013, sugarcane insect pests, particularly sugarcane borers, become increasingly serious due to the prohibition of production and use of terbuthylazine. Therefore, an efficient low-toxicity insecticide which can effectively control the harm of the sugarcane borers is urgently needed. The saccharum officinarum belongs to the family of saccharum officinarum, is one of the main raw materials in sugar industry, and is mainly distributed in southern provinces such as Guangxi, yunnan, guangdong, hainan and the like. In the sugarcane planting process, due to the geographical environment condition limitation, extensive field management and other reasons, sugarcane diseases and insect pests frequently occur, and stem borers are one of common insect pests on the sugarcane. The common sugarcane borers include Diaphlus cruller, huang Ming, white borer, striped borer, greater borer and the like. In the seedling stage, the sugarcane borers are eaten into tender stems of the sugarcane by larvae, invade the tissue of the stems of the sugarcane, destroy growing points and cause dead heart seedlings; the young insects damage the sugarcane stalks in the middle and later growth stages, destroy tissues in the stalks, influence the growth of the sugarcane, lead to the reduction of the yield of the sugarcane, and simultaneously cause the great reduction of the sugar content, thereby directly influencing the economic benefit.

In the planting and production process of the sugarcane, chemical agents such as chlorantraniliprole and the like are sprayed to prevent and control the stem borers. However, pesticides containing a single component often have defects in the process of controlling pesticide pests: the continuous use is easy to cause the pests to generate drug resistance, the insecticidal spectrum is narrow, and the comprehensive protection can not be provided for crops. The pesticide with different action mechanisms is compounded, so that the defects can be overcome to a certain extent, and three action types can appear after compounding: antagonism, addition and synergism, wherein the compounding of synergistic action type expands the insecticidal spectrum, and simultaneously can also improve the control effect and reduce the application amount of pesticide.

The tianweimycin is a new avermectin compound produced by engineering bacteria Streptomyces avermitilis MHJ1011 constructed by gene modification by adopting a combined biosynthesis method, has good control effect on pests such as pest mites and lepidoptera and parasites of livestock, and has a structural general formula of the tianweimycin disclosed in the patent with the application number of CN 201410208660.9.

In the actual process of agricultural production, the most easily generated problem for controlling pests is the generation of drug resistance of pests. The compounding of different kinds of components is a common method for preventing and controlling resistant pests. Different components are compounded, and whether a certain compound has synergism, addition or antagonism is judged according to the actual application effect. In most cases, the compounding effect of the pesticide is additive effect, the compounding with real synergistic effect is less, and especially the compounding with very obvious synergistic effect and very high co-toxicity coefficient is less. The inventor discovers that obvious synergistic effect is shown when the ivermectin and the fluroxypyr, the thiocyclam or the chromafenozide are subjected to binary compounding according to a certain mass ratio through an indoor biological activity test. No report on this aspect has been found so far.

The above background disclosure is only for the purpose of assisting understanding of the inventive concept and technical solutions of the present invention, and does not necessarily belong to the prior art of the present patent application, and should not be used for evaluating the novelty and inventive step of the present application in the case that there is no clear evidence that the above content is disclosed at the filing date of the present patent application.

Disclosure of Invention

The invention aims to provide a synergistic pesticide for preventing and treating sugarcane borers, which aims to solve the problem of using a single-component pesticide in the process of preventing and treating the sugarcane borers.

In order to achieve the purpose, the invention provides the following technical scheme:

a synergistic insecticidal composition for preventing and treating sugarcane borer comprises the active ingredients of oryzanol, flupyradifurone and thiocyclam or chromafenozide; wherein the mass ratio of the ivermectin to the fluropyrone is 1-5:40-1; the mass ratio of the ivermectin to the thiocyclam is 1-20:30-1; the mass ratio of the ivermectin to the chromafenozide is 1-40:20-1.

Preferably, the mass ratio of the ivermectin to the fluropyrone is 2:1.

preferably, the mass ratio of the ivermectin to the thiocyclam is 20:1.

preferably, the mass ratio of the ivermectin to the chromafenozide is 20:1.

the second object of the present invention: the pesticide containing the pesticide composition is provided, the mass of the active ingredient accounts for 1-75% of the total mass of the pesticide, and the balance is an agriculturally and pharmaceutically acceptable auxiliary agent.

Preferably, the agriculturally and pharmaceutically acceptable auxiliary agent is composed of one or more of fillers, wetting agents, emulsifiers, binders, disintegrating agents, dispersing agents, thickening agents, preservatives, defoaming agents, antifreezing agents, solvents and stabilizing agents.

Preferably, the dosage form of the pesticide is any dosage form which is agriculturally allowed to use, and the dosage form comprises wettable powder, water dispersible granules, suspending agents and aqueous emulsion.

For wettable powders, the auxiliaries which can be used are: a dispersant such as one or more of polycarboxylate, lignosulfonate, alkylnaphthalene sulfonate; wetting agents such as one or more of alkyl sulfates, alkyl sulfonates, naphthalene sulfonates; the filler is selected from one or more of ammonium sulfate, urea, sucrose, glucose, diatomaceous earth, attapulgite, light calcium carbonate, pulvis Talci, white carbon black, pottery clay, and kaolin.

For water dispersible granules, the auxiliaries that can be used are: a dispersant such as one or more of polycarboxylate, lignosulfonate, alkylnaphthalene sulfonate; wetting agents such as one or more of alkyl sulfate, alkyl sulfonate, naphthalene sulfonate; disintegrating agent such as one or more of sodium sulfate, urea, sucrose, and glucose; binder such as one or more of diatomaceous earth, corn starch, carboxymethyl cellulose; the filler is one or more of diatomite, kaolin, white carbon black, light calcium carbonate, talcum powder and attapulgite.

For the suspending agents, usable are: dispersing agents such as one or more of polycarboxylate, lignosulfonate; emulsifier such as one or more of Nongru 700#, nongru 2201, sorbitan stearate, tween-60 #, and Nongru 1601 #; wetting agents such as one or more of alkylphenol polyoxyethylene ether formaldehyde condensate sulfate, alkylphenol polyoxyethylene ether phosphate, phenethylphenol polyoxyethylene ether phosphate, alkyl sulfate, alkyl sulfonate, naphthalene sulfonate; thickening agent such as one or more of xanthan gum, polyvinyl alcohol, bentonite, and magnesium aluminum silicate; preservatives such as one or more of formaldehyde, benzoic acid, sodium benzoate; defoaming agents such as silicone defoaming agents; antifreeze agents such as one or more of ethylene glycol, propylene glycol, glycerol, urea, and sodium chloride.

For aqueous emulsions, auxiliaries which can be used are: the emulsifier is one or more of agricultural milk 600# phosphate, agricultural milk 700#, agricultural milk 2201#, span-60#, emulsifier T-60, TX-10, agricultural milk 1601, agricultural milk 400#, and agricultural milk 600 #; solvents such as one or more of xylene and toluene; one or more of stabilizers such as triphenyl phosphite, epichlorohydrin and acetic anhydride; antifreeze agents such as one or more of ethylene glycol, propylene glycol, glycerol, urea, and sodium chloride; thickening agent such as one or more of xanthan gum, polyvinyl alcohol, bentonite, and magnesium aluminum silicate; preservatives such as one or more of formaldehyde, benzoic acid, sodium benzoate.

A third object of the invention: provides the application of the insecticidal composition in preventing and treating sugarcane borers.

A fourth object of the invention: provides the application of the pesticide in preventing and treating sugarcane borers.

Fluopyradifurone (Flupyradifuranone), the chemical name of which is 4- [ (6-chloro-3-pyridylmethyl) (2,2-difluoroethyl) amino ] furan-2 (5H) -one, is a novel nicotinic insecticide which acts on the central nervous system of insects and is an antagonist of the nicotinic acetylcholine receptor of insects. Mainly effective to piercing-sucking mouthpart pests, harmless to bees, not limited by flowering application, and safe to crops. Fluopyradifurone (flupyradifurone) is a novel butenolide compound, has a brand-new chemical structure, a novel action mechanism and low toxicity to bees, can be used for many crops, effectively controls main piercing-sucking mouthpart pests, and is an important tool for pest resistance control. The flupyradifurone is quick and efficient in preventing and treating piercing-sucking mouthpart pests, and is environment-friendly and low in toxicity. Especially, the flupyradifurone is safe to bees, and overcomes the fatal defect of neonicotinoid bionic insecticides, so that the future market of the flupyradifurone is expected.

Thiocyclam (thiocyclam), chemical name: n, N-dimethyl-1,2,3-trithioheterocyclic hex-5-amine and its oxalate salt. The thiocyclam is a nereistoxin insecticide, has the functions of stomach toxicity, contact killing and systemic absorption, can be conducted to the apical, the acting part is cholinergic synapse, and the normal transmission of nerves is blocked, so that the nerves of pests do not react to external stimulation, and when the pests contact or take the insecticide, the pests quickly become immobile and paralyze until the pests die.

Chromafenozide (Chromafenozide), chemical name: N-tert-butyl-N' - (3-methoxy-2-methylbenzoyl) -3,5-dimethylbenzoyl hydrazine. Is a diacylhydrazine pesticide, inhibits the feeding of pests within a few hours after the pests take the feed, and simultaneously causes the pests to molt in advance to die. The chromafenozide is not only low in toxicity to mammals, birds and aquatic organisms, but also has no influence on arthropods, predatory acarids, spiders, hemiptera, coleoptera (beetles), parasites and the environment.

Compared with the prior art, the invention has the following beneficial effects:

the components of the invention are reasonable, and the activity after compounding is not the simple superposition of the activities of all the components, but has obvious synergistic effect. Compared with single pesticide, the pesticide can improve the insecticidal effect, can expand the insecticidal spectrum, and can effectively solve the problem of using single-component pesticide to prevent and control sugarcane borers. And the insecticidal mechanisms of the 2 active ingredients are different during compounding, so that the generation of drug resistance of pests can be delayed, and the pesticide has a certain protection effect on the pesticide.

Detailed Description

The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.

Example 1: indoor biological activity test of dualistic compound of tianweicin

Test subjects: feeding the Diatra nubilalis collected from a sugarcane planting field in a laboratory, and selecting 2-instar larvae as test objects;

reagent to be tested: 98% of avimectin (the mass ratio of avimectin A to avimectin B is 8:2), 96% of fluroxypyr, 90% of thiocyclam and 92% of chromafenozide;

the test method comprises the following steps: ( Reference is made to part 6 of the pesticide laboratory bioassay test guidelines for pesticides NY/T1154.6-2006: insect-soaking method for insecticidal activity test )

1. Dissolving the raw medicine with acetone, diluting with 0.1% Tween-80 water solution to respectively prepare single-dose mother liquor, setting multiple groups of ratios, setting 6 gradient mass concentrations for each single dose and each group of ratio mixture, and setting 50 mL for each mass concentration liquid medicine;

2. soaking 2-instar larvae of Diatra sacchari (Diatra nubilalis) into the medicinal liquid for 10 s, sucking redundant medicinal liquid with filter paper, transferring to normal condition for feeding, repeating each treatment for 4 times, soaking 20 insects for each time, and setting a solution containing only acetone and Tween-80 as a blank control;

3. observing the death condition of the Diatra chilo suppressalis after 24h of treatment, calculating the corrected mortality of each treatment, performing regression analysis on the concentration log values and the corresponding corrected mortality probability values of each single agent and mixed agents with different proportions by using DPS software, and calculating EC ₅₀ And calculating the co-toxicity coefficient (CTC value) of the mixture according to the Sun Yunpei method.

P ₁ = K/N × 100, wherein P ₁ : mortality rate; k: the number of dead insects; n: treating the total number of insects;

P ₂ =[（P _t -P ₀ ）÷（1-P ₀ ）]x 100, wherein, P ₂ : correcting mortality; p _t : (ii) managing mortality; p ₀ : placebo mortality;

observed virulence index (ATI) = (Standard agent EC) ₅₀ Reagent for test EC ₅₀ ）×100；

Theoretical virulence index (TTI) = a agent virulence index x percent of a in the mixture + B agent virulence index x percent of B in the mixture;

co-toxicity coefficient (CTC) = [ actually measured toxicity index (ATI) ÷ theoretical toxicity index of the mixture (TTI) ] × 100.

The criteria are divided by joint action: the co-toxicity coefficient (CTC) is more than or equal to 120, which shows a synergistic effect; the co-toxicity coefficient (CTC) is less than or equal to 80, and the antagonism is shown; 80< co-toxicity coefficient (CTC) <120 showed additive effect. The results are shown in tables 1-3.

TABLE 1 indoor bioactivity assay of Pyverdin and Fluopyranone combinations against Diatra infusorians

Name and proportion of the medicament	EC ₅₀ （mg/L）	ATI	TTI	CTC
					Tianweimycin	2.42	100.00	--	--
Fluopyrafuranones	28.63	125.40	--	--
					1 of ivermectin: fluopyranone 40	10.28	23.54	10.69	220.31
1 of ivermectin: fluopyranone 30	6.49	37.29	11.41	326.92
					1 of ivermectin: fluopyranone 20	3.77	64.19	12.81	501.02
1 of ivermectin: fluopyranone 10	4.82	50.21	16.78	299.30
					1 of ivermectin: fluopyranone 5	2.19	110.50	23.71	466.05
1 of ivermectin: fluopyrafuranone 1	1.91	126.70	54.23	233.65
					And (2) ivermectin 2: fluopyranone 1	0.67	361.19	69.48	519.82
Avermectin 3: fluopyrafuranone 1	2.17	111.52	77.11	144.62
					4, ivermectin: fluopyranone 1	1.06	228.360	81.69	279.47
Avermectin 5: fluopyrafuranone 1	0.84	288.10	84.74	339.97

As can be seen from Table 1, the ratio of the ivermectin to the flupyradifurone is 1-5: the cotoxicity coefficients within the range of 40-1 are all larger than 120, which shows that the biological activity of the compound of the tianweisu and the fluopyram to the sugarcane dibotworm is synergistic effect, especially when the mass ratio is 2:1, the co-toxicity coefficient reaches 519.82, and the synergistic effect is particularly remarkable.

TABLE 2 indoor bioactivity assay of Pyvermectin and thiocyclam combinations against Diatra sacchari

Name and proportion of the medicament	EC ₅₀ （mg/L）	ATI	TTI	CTC
					Tianweimycin	2.42	100.00	--	--
Insecticidal ring	15.46	15.65	--	--
					1 of ivermectin: insecticidal ring 30	7.49	32.31	18.37	175.84
1 of ivermectin: insecticidal ring 25	4.31	56.15	18.90	297.12
					1 of ivermectin: insecticidal ring 20	3.24	74.69	19.67	379.73
1 of ivermectin: insecticidal ring 15	5.29	45.75	20.92	218.62
					1 of ivermectin: insecticidal ring 10	6.84	35.38	23.32	151.71
1 of ivermectin: insecticidal ring 5	4.36	55.50	29.71	186.61
					1 of ivermectin: insecticidal ring 1	3.04	79.61	57.83	137.66
Avermectin 5: insecticidal ring 1	0.97	249.48	85.94	290.29
					And (3) ivermectin 10: insecticidal ring 1	0.83	291.57	92.33	315.78
15 parts of ivermectin: insecticidal ring 1	0.65	372.31	94.73	393.03
					And (3) ivermectin 20: insecticidal ring 1	0.52	465.38	95.98	484.86

As can be seen from Table 2, the mass ratio of the ivermectin to the thiocyclam is 1-20: the co-toxicity coefficients within the range of 30-1 are all larger than 120, which shows that the biological activity of the tianweisu and the thiocyclam after being compounded shows synergistic effect on the sugarcane dibotrys.

TABLE 3 indoor bioactivity assay of Avermectin and Chromafenozide combination on sugarcane Diatra

Name and proportion of the medicament	EC ₅₀ （mg/L）	ATI	TTI	CTC
					Tianweimycin	2.42	100.00	--	--
Chromafenozide	6.58	36.78	--	--
					1 of ivermectin: chromafenozide 20	3.49	69.34	39.79	174.27
1 of ivermectin: chromafenozide 15	2.66	90.98	40.73	223.37
					1 of ivermectin: chromafenozide 10	1.47	164.63	42.53	387.12
1 of ivermectin: chromafenozide 5	1.24	195.16	47.32	412.47
					1 of ivermectin: chromafenozide 3	2.38	101.68	52.58	193.37
1 of ivermectin: chromafenozide 1	2.11	114.69	68.39	167.71
					Avermectin 5: chromafenozide 1	1.03	234.95	89.46	262.62
And (3) ivermectin 10: chromafenozide 1	0.62	390.32	94.25	414.12
					And (3) ivermectin 20: chromafenozide 1	0.46	526.09	96.99	542.42
And (3) ivermectin 30: chromafenozide 1	0.75	322.67	97.96	329.38
					And (3) ivermectin 40: chromafenozide 1	1.16	208.62	98.46	211.89

As can be seen from Table 3, the mass ratio of the ivermectin to the chromafenozide is 1-40: the cotoxicity coefficients within the range of 20-1 are all larger than 120, which shows that the biological activity of the compound of the tianweisu and the chromafenozide to the sugarcane dibbler shows synergistic effect. Especially when the mass ratio is 20:1, the co-toxicity coefficient reaches 542.42, and the synergistic effect is particularly remarkable.

In conclusion, when the tianweimycin, the flupyradifurone and the thiocyclam or the chromafenozide are subjected to binary composition according to a certain mass ratio, the cotoxicity coefficients are all larger than 120, and the synergistic effect is achieved, so that the control effect on the sugarcane borers can be improved, and the problem caused by using a single-component pesticide to control the sugarcane borers is avoided.

It should be noted that, in this document, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications. It is intended that the scope of the invention be defined by the claims and their equivalents.

Claims

1. The synergistic insecticidal composition for preventing and treating sugarcane borers is characterized in that the active ingredient is prepared by compounding tianweisu and thiocyclam; wherein the mass ratio of the ivermectin to the thiocyclam is 1-40:20-1.

2. The synergistic insecticidal composition for preventing and treating sugarcane borer according to claim 1, wherein the mass ratio of the ivermectin to the thiocyclam is 20:1.

3. an insecticide containing the insecticidal composition according to claim 1, wherein the mass of the active ingredient accounts for 1-75% of the total mass of the insecticide, and the balance is an agriculturally pharmaceutically acceptable auxiliary.

4. The insecticide according to claim 3, wherein said agriculturally and pharmaceutically acceptable adjuvants comprise one or more of fillers, wetting agents, emulsifiers, binders, disintegrants, dispersants, thickeners, preservatives, antifoaming agents, anti-freezing agents, solvents, and stabilizers.

5. The insecticide according to claim 3, wherein the formulation of the insecticide is any formulation allowed to be used in agriculture, and comprises wettable powder, water dispersible granules, suspending agents and emulsion in water.

6. The use of the insecticidal composition according to any one of claims 1-2 for the control of sugarcane borers.

7. The use of the insecticide of any one of claims 3 to 5 for the control of sugarcane borers.