CN113925057B

CN113925057B - Insecticidal composition containing nitroinsect sulfur and phosphorus

Info

Publication number: CN113925057B
Application number: CN202111120201.1A
Authority: CN
Inventors: 郑敬敏; 冯建雄; 刘欢
Original assignee: Shaanxi Sunger Road Bio Science Co Ltd
Current assignee: Shaanxi Sunger Road Bio Science Co Ltd
Priority date: 2021-09-24
Filing date: 2021-09-24
Publication date: 2022-07-15
Anticipated expiration: 2041-09-24
Also published as: CN113925057A

Abstract

The invention discloses a pesticide composition containing nitenpyram and spirotetramat, and effective active ingredients of the pesticide composition comprise nitenpyram and spirotetramat, wherein the weight ratio of the nitenpyram to the spirotetramat is 20:1-1: 20. The formulation of the composition is missible oil, aqueous emulsion, microemulsion, suspoemulsion, wettable powder, water dispersible granule, dispersible liquid, microcapsule suspending agent and the like. After the nitenpyram and the spirotetramat are compounded, the synergistic effect is obvious, the pesticide composition has excellent control effect on agricultural pests, can reduce the using amount of the pesticide, reduce the cost, delay the generation of the drug resistance of the pests and prolong the service life of the pesticide.

Description

Insecticide composition containing nitre sulfur and phosphorus

Technical Field

The invention relates to an insecticidal composition containing nitre sulfur and phosphorus, belonging to the technical field of pesticide compounding.

Background

Nitrophos, an organophosphorus insecticide and acaricide independently developed by chemical research and design institute of Sichuan province, has stomach toxicity and contact activity, has better permeability in plant bodies, and mainly plays a role in inhibiting acetylcholinesterase in insect bodies, thereby hindering nerve conduction of pests to cause death of the pests. The prevention and control object registered in China at present is citrus arrowhead scale, and the medicament also has remarkable prevention and control effects on citrus red spider, arrowhead scale, cotton bollworm, vegetable oriental tobacco budworm, diamond back moth, rice planthopper, rice thrips and the like according to the literature.

The spirotetramat is a two-way systemic broad-spectrum pesticide developed by Bayer company, can move upwards and downwards in the whole plant body, has unique systemic performance, can protect new stems, leaves and roots, and prevent and control the growth of eggs and larvae of pests, has the action mechanism of interfering the fat biosynthesis of insects to cause death of the larvae, effectively reduces the reproductive capacity of adults, can effectively prevent and control various piercing-sucking mouthparts pests, such as aphids, thrips, psyllids, mealybugs, whiteflies, scale insects and the like, has long efficacy duration, and can protect crops from being damaged by the pests for a long time.

The compound has good effect when being used alone, but pests can gradually generate resistance along with the prolonging of the using time, farmers can increase the using amount of pesticides along with the generation of the resistance of the pests, so that the using amount of the pesticides can be greatly increased in a vicious circle and the pesticides gradually lose the effect on the pests, and the alternate use or the mixed use of the pesticides is an effective method for solving the generation of the resistance of the pests. Meanwhile, the blending of the two pesticides can generally generate three different results, namely antagonism, addition or synergism, so that the synergistic pesticide composition can be effectively screened out, the generation of pest resistance can be delayed, the pesticide use amount can be effectively reduced, the cost is low, and the environment is protected.

Disclosure of Invention

The invention aims to provide an insecticidal composition with synergistic effect, which comprises the following components:

(1) a first active ingredient: nitenpyram;

(2) the second active ingredient: spirotetramat;

the invention also aims to provide application of the composition in preventing and controlling pests and mites on crops.

In order to achieve the purpose, the technical scheme of the invention is as follows:

the invention relates to an insecticidal composition containing nitrofos, wherein the weight ratio of active ingredients of the nitrofos to spirotetramat is 20:1-1:20, preferably 20:1-1:15, more preferably 5: 1-3: 1. in the insecticidal composition, the total weight of the effective components accounts for 1-90% of the weight of the pesticide composition, and the balance is an auxiliary agent.

The invention relates to an insecticidal composition containing nitrofos, which can be prepared into different formulations suitable for agricultural production according to the method known by technical personnel in the technical field, and the preferable formulations comprise missible oil, aqueous emulsion, microemulsion, suspoemulsion, wettable powder, water dispersible granules, dispersible liquid and microcapsule suspending agent.

For emulsifiable concentrates, the auxiliaries which can be used are: emulsifiers such as Nongru 500#, Nongru 700#, Nongru 2201, span-60 #, emulsifier T-60, TX-10, Nongru 1601#, Nongru 600#, Nongru 400 #; solvents such as xylene, mineral spirits, toluene, biodiesel, methyl esterified vegetable oils, N-methyl pyrrolidone; cosolvents such as ethyl acetate, sec-butyl acetate, methanol, dimethylformamide, cyclohexanone, acetone, methyl ethyl ketone; stabilizers such as triphenyl phosphite, epichlorohydrin, acetic anhydride.

For aqueous emulsions, the auxiliaries which can be used are: emulsifier such as nonylphenol polyoxyethylene ether phosphate, triphenylethylphenol polyoxyethylene ether phosphate, Nongru No. 700, Nongru No. 2201, span-60, Tween-60, TX-10, Nongru 1601, Nongru No. 600, Nongru No. 400; one or more solvents such as xylene, toluene, cyclohexanone and solvent oil; one or more stabilizers such as triphenyl phosphite, epichlorohydrin and epoxidized soybean oil; an antifreezing agent: one or more of ethylene glycol, propylene glycol, glycerol, urea, and inorganic salts such as sodium chloride; thickening agent such as one or more of xanthan gum, polyvinyl alcohol, bentonite, and magnesium aluminum silicate; the antiseptic is one or more of formaldehyde, benzoic acid, and sodium benzoate, and the water is deionized water.

For microemulsions, the auxiliaries which can be used are: emulsifier Nongru 500#, Nongru 700#, Nongru 2201, span-60 #, Tween 60#, TX-10, Nongru 1601, Nongru 600#, Nongru 400 #; the auxiliary emulsifier is selected from methanol, isopropanol, n-butanol, and ethanol; the solvent is selected from cyclohexanone, N-methyl pyrrolidone, xylene, toluene and solvent oil; the stabilizer is selected from triphenyl phosphite and epichlorohydrin; the water is deionized water.

For suspoemulsions, the auxiliaries which can be used are: the emulsifier is one or more of agricultural milk 500#, agricultural milk 700#, agricultural milk 2201, span-60 #, emulsifier T-60, TX-10, agricultural milk 1601#, agricultural milk 600#, and agricultural milk 400 #; a dispersant such as one or more of polycarboxylate, lignosulfonate, alkylnaphthalene sulfonate; solvent such as one or more of dimethyl phthalate, dibutyl phthalate, ethyl acetate and methyl benzoate; defoaming agents such as silicone oil, silicone compounds, C10-20 saturated fatty acid compounds, C8-10 fatty alcohols, hexanol, butanol, octanol; thickening agent such as one or more of white carbon black, polyvinyl alcohol, bentonite, and magnesium aluminum silicate; antifreeze agent such as one or more of ethylene glycol, propylene glycol, glycerol, urea, inorganic salts such as sodium chloride; the water is deionized water.

For wettable powders, the auxiliaries which can be used are: dispersing agents such as one or more of polycarboxylate, lignosulfonate, polyoxyethylene polyoxypropylene ether block copolymer, alkyl naphthalene formaldehyde condensate sulfonate, alkylphenol polyoxyethylene ether phosphate, fatty alcohol polyoxyethylene ether phosphate and alkylphenol polyoxyethylene ether sulfonate; wetting agents such as one or more of alkyl sulfate, alkyl sulfonate, alkyl naphthalene sulfonate; the filler is selected from one or more of ammonium sulfate, urea, sucrose, glucose, diatomaceous earth, kaolin, white carbon black, light calcium carbonate, pulvis Talci, attapulgite, and pottery clay.

For water dispersible granules, the following additives can be used: a dispersant such as one or more of polycarboxylate, lignosulfonate, alkylnaphthalene sulfonate; wetting agents such as one or more of alkyl sulfate, alkyl sulfonate, naphthalene sulfonate; disintegrating agent such as one or more of ammonium sulfate, sodium sulfate, polyvinylpyrrolidone, starch and its derivatives, and bentonite; binder such as one or more of starch, glucose, polyvinyl alcohol, polyethylene glycol, sodium carboxymethylcellulose, and sucrose; the filler is one or more of diatomite, kaolin, white carbon black, light calcium carbonate, talcum powder, attapulgite and argil.

For dispersible agents, the auxiliaries that can be used are: solvents such as one or more of dimethyl phthalate, dibutyl phthalate, ethyl acetate, methyl benzoate, acetone, alkyl pyrrolidone, ethanol, isopropanol, isobutanol; emulsifier such as one or more of Nongru 500#, Nongru 700#, Nongru 2201, span-60 #, emulsifier T-60, polyoxyethylene octylphenol ether, Nongru 1601#, Nongru 600# and Nongru 400 #; vegetable oil methyl ester such as palm oil methyl ester, coconut oil methyl ester, soybean oil methyl ester, and methyl oleate.

For the suspension of microcapsules, the auxiliaries which can be used are: high molecular capsule wall material such as one or more of polyfunctional acyl halide polyamine, gelatin, acacia, modified milk protein, and sodium alginate; dispersing agent such as fatty alcohol polyoxyethylene ether, alkylphenol polyoxyethylene formaldehyde condensate sulfate, and alkylbenzene sulfonate; solvent such as one or more of fatty acid ester, oleum Lini, and methyl oleate; emulsifiers such as one or more of benzyl dimethyl phenol polyoxyethylene ether, phenethyl phenol polyoxyethylene polypropylene ether, and ethylene oxide-propylene oxide block copolymer; pH regulator such as sodium hydroxide and one or more of hydrochloric acid, citric acid, and sorbic acid; defoaming agents such as one or more of C8-10 fatty alcohols, amides, silicone defoamers, hexanols, butanols, octanols; the water is deionized water.

The production process of various application formulations of the pesticide composition and the types of the auxiliary agents belong to the prior known technology, and are not described in detail herein.

The insecticidal and acaricidal composition can be used for controlling various crop pests. Such as mites, scale insects, psyllids, aphids, whiteflies, thrips, etc.

The invention can achieve the following technical effects:

1. the two compounded active ingredients have different action mechanisms, and can delay the generation of drug resistance of pests;

2. after the two compounds are compounded, the synergistic effect is obvious, the using amount of pesticides can be reduced, and the cost is reduced;

3. the usage amount of pesticides is reduced, and the environment is protected.

Detailed Description

Indoor toxicity measurement is used for explaining indoor toxicity activity of mixed nitenpyram and pyriproxyfen on two-spotted spider mite, diaphorina citri and apple cotton aphid.

1. Indoor toxicity determination method for tetranychus urticae by mixing nitrothion and spirotetramat

The test method comprises the following steps: firstly, preparing the liquid medicine to be detected into the required concentration for standby. Preparing a test material, cutting a double-sided adhesive tape into 2cm long, sticking the test material to one end of a glass slide, selecting healthy female adult mites which are bred indoors by using a fine hair pen, sticking the backs of the healthy female adult mites to the double-sided adhesive, paying attention to no adhesion of mite feet, tentacles and mouthparts, placing each piece of the female adult mites with 30 heads, placing the female adult mites into a container padded with wet filter paper, culturing the female adult mite at the temperature of 25 +/-1 ℃, performing microscopic examination after 2 hours, removing dead and injured individuals, and supplementing 30 heads. Placing the glass slide in the prepared liquid medicine, slightly shaking for 5s, taking out, sucking off the redundant liquid medicine by using absorbent paper, placing the glass slide in a container filled with wet filter paper, and placing the glass slide in a normal condition for feeding and observation. Each treatment was repeated 4 times, and the treatment with clear water spray was set as a blank control. And (5) checking the death condition of the test insects 48 hours after treatment, and respectively recording the total number of the insects and the number of the dead insects.

2. Indoor toxicity determination of mixing of nitre sulfur and spirotetramat on diaphorina citri

The test method comprises the following steps: firstly, preparing the liquid medicine to be detected into the required concentration for standby. Respectively soaking fresh citrus leaves in the liquid medicine to be detected for 15s, taking out, wrapping leaf stalks with wet cotton, placing in a disposable cup, inoculating the hungry treated test insects, repeating for 4 times each time, repeating for about 30 times each time, and setting clear water as blank control. And (5) checking the death condition of the test insects 48 hours after treatment, and respectively recording the number of the test insects and the number of the dead insects.

3. Indoor toxicity determination method for apple cotton aphids by mixing nitrothion and spirotetramat

The test method comprises the following steps: firstly, preparing the liquid medicine to be detected into the required concentration for standby. Soaking apple branches with cotton aphids in the liquid medicine for 10s, taking out, sucking redundant liquid medicine with filter paper, then putting into an insect-raising box for normal feeding, repeating for 4 times every treatment, and setting clear water for treating as blank control every 25 times of repeated cotton aphids. And (5) checking the death condition of the test insects 48 hours after treatment, and respectively recording the total number of the insects and the dead number of the insects.

Solving a regression equation by using the logarithm of the concentration of the medicament and the control effect rate value, and calculating to obtain LC of each treatment₅₀Evaluating the combined action of the compound medicament on each target by a co-toxicity coefficient method>120 is expressed as synergistic effect, the co-toxicity coefficient is expressed as additive effect between 80 and 120, and the co-toxicity coefficient<80 is shown as antagonistic.

Mortality (%) = number of dead insects (eggs)/number of total insects (eggs) treated × 100

Corrected mortality (%) = (treatment mortality-blank)/(1-blank mortality) × 100

TABLE 1 toxicity test results of mixing of nitrothion and spirotetramat on Tetranychus urticae

As can be seen from the table 1, the compounding of the nitenpyram and the spirotetramat in the ratio of 20:1-1:15 has certain synergistic effect, wherein the cotoxicity coefficient is the largest when the ratio is 4:1, and the synergistic effect is the most obvious.

TABLE 2 toxicity test results of mixing of Nitrophos and Spirotetramat against diaphorina citri

As can be seen from the table 2, the compounding of the nitenpyram and the spirotetramat in the ratio of 20:1-1:20 has certain synergistic effect, wherein the cotoxicity coefficient is the largest when the ratio is 5:1, and the synergistic effect is the most obvious.

TABLE 3 toxicity test results of mixing of nitrofos and spirotetramat on Aphis citricola

As can be seen from Table 3, the compounding of the nitenpyram and the spirotetramat in the ratio of 20:1-1:15 has a certain synergistic effect, wherein the cotoxicity coefficient is the largest and the synergistic effect is the most obvious when the ratio is 5: 1.

The present invention is further illustrated by the following formulation examples.

Formulation example 1

40% of nitrobenfofos, 10% of spirotetramat, 700# 7% of Nongru, 1601# 3% of Nongru, 5% of span-60 # 5%, 5% of cyclohexanone, 2% of N-methyl pyrrolidone, 6% of sec-butyl acetate, 2.5% of triphenyl phosphite and 100% of solvent oil S-150# by weight. The above-mentioned raw materials are mixed, stirred and dissolved completely so as to obtain 50% nitro-insect sulfur-phosphorus spirotetramat emulsifiable solution.

Formulation example 2

40% of nitrofos, 10% of spirotetramat, 5% of tween-60 #, 4% of cyclohexanone, 2% of epoxidized soybean oil, 2% of ethylene glycol, 5% of polyvinyl alcohol, 1.5% of formaldehyde and deionized water in 100% by weight. The raw materials are uniformly mixed and stirred at a high speed to prepare the 50 percent aqueous emulsion of the nifedipine and the spirotetramat.

Formulation example 3

The pesticide composition comprises, by weight, 50% of nitrobenfop, 10% of spirotetramat, 5% of agricultural emulsion 700#, 22015% of agricultural emulsion, 2.5% of methanol, 4% of N-methyl pyrrolidone, 1% of triphenyl phosphite and 100% of deionized water. The above raw materials are stirred and mixed to prepare the 60 percent of the nitenpyram-spirotetramat microemulsion.

Formulation example 4

50% of nitrofos, 10% of spirotetramat, 3.5% of triphenylethylphenol polyoxyethylene ether phosphate, 2.5% of dispersing agent NNO, 3% of sodium polycarboxylate, 3% of castor oil, 2% of methyl oleate, 2.5% of tween-60 #, TERSPERSE 25003% of dispersing agent, 3% of bentonite, 1% of polyvinyl alcohol, 5% of ethylene glycol, 1% of epoxy chloropropane, 0.5% of formaldehyde, 0.5% of butanol and deionized water added to 100% by weight. The raw materials are uniformly mixed, sheared at a high speed and sanded to prepare the 60 percent suspending emulsion of the nitenpyram and the spirotetramat.

Biological example 1: field efficacy test for controlling tetranychus urticae koch

In 2021, the inventors performed efficacy tests of formulation example 1, formulation example 2, formulation example 3, formulation example 4, and a control agent for controlling tetranychus urticae, and the test results are shown in table 4, in which the test substance was capsicum.

The test method comprises the following steps: the method comprises the steps of randomly arranging all cells, repeating the treatment for 4 times every 20 square meters in each cell, additionally arranging blank comparison, wherein the application method is a common spraying method, investigating the population base number before application, investigating the number of live insects 1, 3 and 7 days after application, investigating the investigating method comprises the steps of sampling 5 points in each cell, fixing two hot peppers in each point, fixing 5 leaves on the middle upper part of each hot pepper, and investigating the number of live insects on the fixed leaves.

The drug effect calculation method comprises the following steps:

oral cavity decline rate (%) = (number of insects before application-number of insects after application)/number of insects before application × 100

Control effect (%) = (treatment area population reduction rate-control area population reduction rate)/(1-control area population reduction rate) × 100

TABLE 4 Effect on Tetranychus urticae Koch

From table 4, the field control effect of the preparation example compounded by the nitenpyram and the spirotetramat on the capsicum is higher than that of each single agent in 1, 3 and 7 days after the application, and the synergistic effect of the two agents after the compounding can be further verified.

During the test period, all the tested agents are safe to the growth of the hot pepper and have no phytotoxicity.

Biological example 2: field efficacy test for controlling diaphorina citri

The inventor carries out field efficacy tests on the diaphorina citri in 2021, wherein the test results are shown in Table 5, and the test results are shown in formulation example 1, formulation example 2, formulation example 3, formulation example 4 and a control medicament.

The test method comprises the following steps: 3 citrus trees in each cell are treated for 3 times, and the cells are randomly arranged and are additionally provided with blank controls. The application method is spray method, insect population base number is investigated before application, live insect number is investigated 3, 7, 14 and 21 days after application, tips are positioned at east, south, west, north and middle five positions of each citrus tree, 2 tips are positioned at each position, and live insect number is recorded.

The efficacy of the drug was calculated in the same manner as in biological example 1.

TABLE 5 control Effect on diaphorina citri

From table 5, the field control effect of the preparation example compounded by the nitenpyram and the spirotetramat 3, 7, 14 and 21 days after the application of the preparation is higher than that of each single agent, and the synergistic effect of the two after the compounding can be further proved.

And (4) observing during the test period, wherein all the test agents are safe to the growth of the citrus and have no phytotoxicity phenomenon.

Biological example 3: field pesticide effect test for preventing and controlling apple green peach aphids

The inventor carries out field pesticide effect tests on Aphis pomi in 2021, wherein the field pesticide effect tests are carried out on the preparation example 1, the preparation example 2, the preparation example 3, the preparation example 4 and a control medicament, and the test results are shown in Table 6.

The test method comprises the following steps: 2 apple trees in each cell are treated for 3 times, and the cells are randomly arranged and are additionally provided with blank controls. The pesticide application method is a spraying method, insect population base number is investigated before pesticide application, the number of live insects is investigated 3, 7, 14 and 21 days after pesticide application, tips are positioned in five directions of east, south, west, north and middle of each apple tree during investigation, 2 tips are positioned in each direction, and the number of live insects is recorded.

The efficacy was calculated in the same manner as in biological example 1.

TABLE 6 prevention and control of Aphis citricola

From table 6, the field control effect of the preparation example compounded by the nitenpyram and the spirotetramat is higher than that of each single agent in 3, 7, 14 and 21 days after the pesticide application, and the synergistic effect of the two compounded agents can be further proved.

During the test period, all the tested reagents are safe to apple growth and have no phytotoxicity.

In conclusion, after the nitenpyram and the spirotetramat are compounded, the two active ingredients have unexpected synergistic effect in a certain proportion, and the composition can be prepared into various agriculturally acceptable formulations, can be well applied in fields, has obvious control effect on various agricultural pests, can reduce the dosage and the drug cost, is safe to crops, and is worthy of popularization and application in agricultural production practice.

Claims

1. An insecticidal composition containing nitrofos, which is characterized in that: the effective active ingredients comprise the nitenpyram and the spirotetramat, and the mass ratio of the nitenpyram to the spirotetramat is 20:1-1: 15.

2. the insecticidal composition containing nitrofos according to claim 1, characterized in that: the mass ratio of the nitenpyram to the spirotetramat is 5: 1-3: 1.

3. the insecticidal composition containing nitrofos according to claim 1, characterized in that: the total weight of the nitenpyram and the spirotetramat accounts for 1 to 90 percent of the total weight of the composition in percentage by mass.

4. The insecticidal composition containing nitrofos according to claim 1, characterised in that: the composition can be prepared into missible oil, aqueous emulsion, microemulsion, suspension emulsion, wettable powder, water dispersible granules, dispersible liquid and microcapsule suspending agent.

5. The use of the insecticidal composition containing nitrofos according to any one of claims 1 to 4 for controlling tetranychus urticae, diaphorina citri and aphis gossypii glover.