CN115518130A - Traditional Chinese medicine formula for treating gout and preparation method thereof - Google Patents

Traditional Chinese medicine formula for treating gout and preparation method thereof Download PDF

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CN115518130A
CN115518130A CN202211133731.4A CN202211133731A CN115518130A CN 115518130 A CN115518130 A CN 115518130A CN 202211133731 A CN202211133731 A CN 202211133731A CN 115518130 A CN115518130 A CN 115518130A
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pretreated
traditional chinese
chinese medicine
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medicine formula
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罗利芝
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Xishuangbanna Daimi Pharmaceutical Co ltd
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Xishuangbanna Daimi Pharmaceutical Co ltd
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    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
    • A61K36/488—Pueraria (kudzu)
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    • A61K35/57—Birds; Materials from birds, e.g. eggs, feathers, egg white, egg yolk or endothelium corneum gigeriae galli
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Abstract

The invention relates to the technical field of traditional Chinese medicines and discloses a traditional Chinese medicine formula for treating gout and a preparation method thereof. The traditional Chinese medicine formula for treating gout comprises a traditional Chinese medicine mixture and composite probiotics; the traditional Chinese medicine extract powder comprises: radix Puerariae, rhizoma Phragmitis, fructus Pyri Opuntiae Dillenii, old rice, plantago ovata forsk shell, lalang grass rhizome, cortex Cinnamomi, retinervus Luffae fructus, bulbus Lilii, endothelium corneum Gigeriae Galli, fructus Chaenomelis, folium Mori, sunflower disc, flos Abelmoschi Manihot, acer Truncatum Bunge oil, and fructus Citri Limoniae powder; the composite probiotics comprise lactobacillus plantarum, bifidobacterium bifidum and clostridium butyricum. The traditional Chinese medicine formula disclosed by the invention has the effects of regulating spleen and stomach, nourishing liver and kidney, dredging channels, relieving depression, promoting blood circulation, removing blood stasis, dredging channels and relieving pain. The traditional Chinese medicine mixture in the traditional Chinese medicine formula provided by the invention is cooperated with the composite probiotics, so that the treatment effect of gout can be further improved.

Description

Traditional Chinese medicine formula for treating gout and preparation method thereof
Technical Field
The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine formula for treating gout and a preparation method thereof.
Background
Gout is a common and complex type of arthritis, which can be suffered by all ages, with higher incidence in men than women. Patients with gout often have sudden joint pain at night, the joint is in urgent attack, severe pain, edema, red swelling and inflammation appear at the joint, and the pain is slowly relieved until the pain disappears for days or weeks. When pain occurs, the patient wakes up in a deep sleep in the middle of the night, and the patient describes the pain feeling as if the big toe is burned. The affected joints are red, swollen and inflamed finally, tissues are soft after edema, activities are limited, and daily life is affected finally.
The fundamental cause of gout induction is the accumulation of uric acid in the body, mainly in the leg joints, where crystals of uric acid accumulate, and then white blood cells react to the crystals at the time of their detachment, thus causing inflammation and severe pain. The gout has high morbidity and high recurrence rate after recovery, and is accompanied with various complications. At present, western medicine treatment medicines can be generally divided into three types according to action characteristics: firstly, anti-gout attack drugs; second, a uric acid excreting agent; thirdly, a uric acid synthesis blocking agent. Although the three western medicines can temporarily achieve obvious effects, the three western medicines have large side effects, are commonly seen in gastrointestinal dysfunction, liver and kidney function damage, rash, dermatitis and the like, and are easy to relapse particularly after medicine withdrawal. The traditional Chinese medicine considers that gout is caused by congestion of a corresponding part of the liver channel of a human body and heat arthralgia, so that the common prescription adopts the general prescription for treating gout by promoting blood circulation to remove blood stasis and dredging collaterals to relieve pain, but the effect is slow and the gout is easy to relapse, so that a traditional Chinese medicine formula which can take effect quickly and can radically cure the gout is needed to solve the problems.
Disclosure of Invention
In order to solve the technical problems, the invention provides a traditional Chinese medicine formula for treating gout, which comprises a traditional Chinese medicine mixture and composite probiotics; the traditional Chinese medicine extract powder comprises: radix Puerariae, rhizoma Phragmitis, fructus Pyri Opuntiae Dillenii, old rice, plantago ovata forsk shell, lalang grass rhizome, cortex Cinnamomi, retinervus Luffae fructus, bulbus Lilii, endothelium corneum Gigeriae Galli, fructus Chaenomelis, folium Mori, sunflower disc, flos Abelmoschi Manihot, acer Truncatum Bunge oil, and fructus Citri Limoniae powder; the composite probiotics comprise lactobacillus plantarum, bifidobacterium bifidum and clostridium butyricum.
Kudzu root: property and taste: sweet and pungent in flavor and cool in nature; meridian tropism: entering lung and stomach meridians; the efficacy is as follows: relieving muscles and fever, promoting eruption, promoting fluid production to quench thirst, invigorating yang and relieving diarrhea; the main indications are as follows: can be used for treating fever due to exterior syndrome, pain of neck and back, measles without adequate eruption, thirst due to fever, diabetes due to yin deficiency, dysentery due to heat-purging, and diarrhea due to spleen deficiency.
Reed rhizome: nature and taste: sweet and cold; meridian tropism: the lung and stomach meridians entered: the efficacy is as follows: clearing heat and purging fire, promoting the production of body fluid to quench thirst, relieving restlessness, arresting vomiting and promoting urination: the main treatment is as follows: can be used for treating pyrexia polydipsia, lung heat dry cough, internal heat diabetes, pyocutaneous disease and pyogenic infection.
Pear fruit cactus: property and taste: is bitter in taste; cold in nature; meridian tropism: spleen and lung meridians: the efficacy is as follows: clearing lung-heat to relieve cough; cooling blood and removing toxic substance; the main treatment is as follows: can be used for treating cough due to lung heat, pulmonary tuberculosis, hemoptysis, dysentery, hemorrhoidal bleeding, acute mastitis, mumps, carbuncle, toxic swelling, scald, burn, alopecia, scabies, and snake and insect bite.
Aging rice: property and taste: sweet, bland and mild; the efficacy is as follows: is semen oryzae Sativae of Gramineae. Can be used for treating weakness of spleen and stomach, anorexia, diarrhea, regurgitation, dysentery, and polydipsia; the meridians: stomach, large intestine, spleen channel; the main treatment is as follows: regulate the middle energizer and regulate the stomach, excrete dampness and check diarrhea, relieve restlessness. It is mainly indicated for weakness of spleen and stomach, poor appetite, diarrhea, regurgitation, dysentery with vomiting, polydipsia.
Plantago ovata husk is rich in pectin (Mucilage, psyllium hydrophilic gum), and comprises arabinose, xylose, galacturonic acid, semi-dry fatty oil, and small amount of Corallin (Aucubine). Psyllium husk contains 80% fiber, while other fiber-rich grains such as oat and wheat bran contain only 15% and 10% fiber, respectively; other nutritional components mainly include glucoside, protein, polysaccharide, vitamin B1 and choline.
Rhizoma imperatae: property and taste: sweet and cold; meridian tropism: the lung, stomach and bladder meridians entered: the efficacy is as follows: cool blood to stop bleeding, clear heat and induce diuresis; the main indications are as follows: can be used for treating hematemesis due to blood heat, epistaxis, hematuria, fever polydipsia, cough due to lung heat, stomach heat emesis, jaundice due to damp-heat pathogen, edema oliguria, stranguria with astringency and pain.
Cinnamon: nature and taste: pungent and sweet in flavor and strong in heat; meridian tropism: entering kidney, spleen, heart and liver meridians; the functions are as follows: has the effects of tonifying fire, supporting yang, guiding fire to the origin, dispelling cold, relieving pain and warming and dredging channels and collaterals: the main indications are impotence, cold womb, cold pain in waist and knees, asthma due to deficiency of the kidney, upward floating of deficient yang, dizziness, conjunctival congestion, cold pain in heart and abdomen, vomiting and diarrhea due to deficiency cold, cold hernia, abdominal pain, dysmenorrheal and amenorrhea.
Loofah sponge: nature and taste: sweet in taste and neutral in nature; the book materia Medica is just written: sweet taste and cold nature; meridian tropism including stomach, lung and liver meridian; the functions are as follows: has effects of dispelling pathogenic wind, dredging collaterals, promoting blood circulation, and promoting lactation; the main indications are as follows: can be used for treating arthralgia, spasm, distending pain of chest and hypochondrium, galactostasis, and mammary abscess with swelling and pain.
Lily: nature and taste: sweet taste and cold nature; meridian tropism: heart and lung meridian entered; the efficacy is as follows: nourishing yin and moistening lung, clearing away heart-fire and tranquilizing; the main indications are as follows: can be used for treating yin deficiency and dry cough, fatigue cough and hemoptysis, dysphoria, palpitation, insomnia, dreaminess, absentmindedness.
Endothelium corneum gigeriae galli: nature and taste: sweet and mild; meridian tropism: it enters spleen, stomach, small intestine and bladder meridians; the efficacy is as follows: strengthening stomach, promoting digestion, arresting seminal emission, relieving stranguria and removing calculus: the main indications are as follows: can be used for treating dyspepsia, emesis, dysentery, infantile malnutrition, enuresis, nocturnal emission, stranguria with stone, pain, gallbladder distention, and hypochondriac pain.
Pawpaw: nature and taste: acid and warm; meridian tropism: entering liver and spleen meridians; the functional indications are as follows: calming the liver, relaxing muscles and tendons, regulating the stomach and eliminating dampness; can be used for treating damp arthralgia, spasm, soreness, pain of waist and knee joint, vomiting, diarrhea, spasm, loempe, and edema.
Mulberry leaf: cold in nature, sweet and bitter in taste; meridian tropism: entering lung and liver meridians; the efficacy is as follows: dispelling wind and heat, clearing lung-heat, moistening dryness, removing liver fire and improving eyesight; can be used for treating wind-heat type common cold, cough due to lung heat, headache, dizziness, conjunctival congestion, vertigo, and hematemesis due to blood heat.
Sunflower discs: it is cold in nature and sweet in taste; entering liver meridian; the efficacy is as follows: clearing heat, relieving pain, calming liver and stopping bleeding; it also has effects in lowering blood pressure, dilating blood vessel, relieving cough, eliminating phlegm, cooling blood, and tonifying heart.
Abelmoschus manihot or abelmoschus manihot: entering kidney meridian and bladder meridian; the efficacy is as follows: clearing heat and eliminating dampness, reducing swelling and promoting urination, and clearing away heat and toxic materials; the okra contains special ingredients with medicinal effects, and can strengthen the kidney and tonify deficiency.
Acer truncatum oil: the efficacy is as follows: dispelling pathogenic wind and removing dampness, promoting blood circulation and removing obstruction in collaterals, relieving swelling and pain, promoting diuresis, clearing heat and detoxicating, dispersing stagnated liver qi, resisting bacteria and resisting tumor; has obvious regulating effect on rheumatic arthritis, rheumatoid arthritis, gout, numbness of limbs, tetany, abdominal pain, female irregular menstruation and the like.
Lemon powder: lemon juice: bitter in nature, warm and nontoxic; lemon fruit and peel juice have effects of promoting fluid production, relieving summer heat, promoting appetite, preventing cardiovascular diseases, clearing heat, eliminating phlegm, resisting bacteria, relieving inflammation, delaying aging, and inhibiting pigmentation.
The invention discloses a preparation method of a traditional Chinese medicine mixture, which comprises the following steps:
(1) Cleaning radix Puerariae, rhizoma Phragmitis, opuntia ficus-indica, chencang rice, plantago depressa seed husk, lalang grass rhizome, cortex Cinnamomi, retinervus Luffae fructus, bulbus Lilii, endothelium corneum Gigeriae Galli, fructus Chaenomelis, folium Mori, helianthus annuus disc, and flos Abelmoschi Manihot, drying at constant temperature, and sterilizing to obtain pretreated radix Puerariae, pretreated rhizoma Phragmitis, pretreated Opuntia ficus-indica, pretreated Chencang rice, pretreated Plantago depressa seed husk, pretreated lalang grass rhizome, pretreated cortex Cinnamomi, pretreated retinervus Luffae fructus, pretreated Bulbus Lilii, pretreated endothelium corneum Gigeriae Galli, pretreated fructus Chaenomelis, pretreated folium Mori, pretreated Helianthus annuus disc, and pretreated flos Abelmoschi Manihot flower;
(2) Mixing 38-46 parts of pretreated radix puerariae, 18-26 parts of pretreated rhizoma phragmitis, 24-32 parts of pretreated pome cactus, 27-35 parts of pretreated rice, 18-26 parts of pretreated lalang grass rhizome, 8-16 parts of pretreated cinnamon, 8-16 parts of pretreated loofah sponge, 8-16 parts of pretreated lily, 6-14 parts of pretreated endothelium corneum gigeriae galli, 6-14 parts of pretreated pawpaw, 6-14 parts of pretreated mulberry leaf, 8-16 parts of pretreated sunflower disc and 8-16 parts of pretreated flower of abelmoschus manihot to obtain a pretreated traditional Chinese medicine mixture, adding the traditional Chinese medicine mixture into water, soaking, heating, continuing to decoct after the water is boiled, and filtering to obtain a first filtrate; decocting the residue with water for the second time, boiling, decocting, and filtering to obtain second filtrate; mixing the second filtrate and the first filtrate, vacuum rotary evaporating to concentrate the Chinese medicinal liquid until the solid content is above 25%, spray drying, pulverizing, and sieving to obtain Chinese medicinal extract powder.
(3) Taking 25-45 parts of lemon powder and 8-16 parts of acer truncatum oil for later use; taking 14-22 parts of pretreated Plantago ovata forsk, grinding, sieving to obtain Plantago ovata forsk powder, and mixing Plantago ovata forsk powder, lemon powder, chinese medicinal extract powder and Acer truncatum oil to obtain a Chinese medicinal mixture.
Preferably, the conditions of the constant temperature drying are as follows: drying at constant temperature of 45-60 ℃ for 3-5h, and sterilizing: sterilizing under ultraviolet lamp with power of 10-15w for 15-30min.
Preferably, during the preparation of the first filtrate: the mass ratio of the traditional Chinese medicine mixture to the water is as follows: 1, 10-15, soaking time: 40-60min, decocting time: 30-60min; in the preparation process of the second filtrate: the mass ratio of the filter residue to the water is as follows: 1:5-10, decoction time: 30-45min.
Preferably, the conditions of the spray drying are: the air inlet temperature is controlled to be 120-150 ℃, the air outlet temperature is controlled to be 90-100 ℃, the rotation speed of the atomizer is 45-50r/min, and the feeding speed is 3-6mL/min.
The invention also discloses a culture method of the composite probiotics, which comprises the following steps:
inoculating lactobacillus plantarum from a solid culture medium by using an inoculating loop into an MRS broth culture medium, placing the MRS broth culture medium in a constant temperature oscillator at 35-37 ℃, and carrying out aerobic culture at 200-220rpm until the culture reaches a stationary phase to obtain a seed solution of the lactobacillus plantarum; inoculating Bifidobacterium bifidum and Clostridium butyricum into MRS and RCM liquid culture medium, respectively, and anaerobically culturing at 35-37 deg.C to stationary phase to obtain seed solutions of Bifidobacterium bifidum and Clostridium butyricum, respectively; mixing the seed liquid of lactobacillus plantarum, bifidobacterium bifidum and clostridium butyricum according to the volume ratio of 2-4:2-4:1-3 to obtain the composite probiotic seed liquid.
The invention also discloses a preparation method of the traditional Chinese medicine formula for treating gout, which comprises the following steps: adding water 1-2 times the total weight of the Chinese medicinal mixture, and nutrient substrate 5-30% of the total weight of the Chinese medicinal mixture and water mixture as fermentation matrix, mixing, and autoclaving at 115-125 deg.C for 15-30min; cooling, inoculating composite probiotic seed liquid accounting for 20-40% of the total mass of the fermentation substrate, hermetically fermenting at 35-37 ℃ for 48-60h, air drying, pulverizing, and sieving with a 60-100 mesh sieve to obtain the traditional Chinese medicine formula for treating gout.
Preferably, the mass ratio between the components of the nutrient substrate is: 1-2 parts of corn starch, brown sugar, fructo-oligosaccharide, lysine and talcum powder, wherein the corn starch is 2-4:2-4:2-3; the number of viable bacteria contained in the traditional Chinese medicine formula for treating gout is not less than 1 × 109CFU/g.
Preferably, the traditional Chinese medicine formula for treating gout is prepared into pharmaceutically acceptable capsules, granules, powder, pills, tablets and oral liquid preparations by the prior art.
Preferably, the traditional Chinese medicine formula for treating gout is prepared by the preparation method of the traditional Chinese medicine formula for treating gout.
Compared with the prior art, the invention has the beneficial effects that:
1. the traditional Chinese medicine formula disclosed by the invention has the effects of regulating spleen and stomach, nourishing liver and kidney, dredging channels, relieving depression, promoting blood circulation, removing blood stasis, dredging channels and relieving pain. The traditional Chinese medicine for treating gout comprises the following components in formula: the reed rhizome and the prickly pear have synergistic effect on detumescence; radix Puerariae, cortex Cinnamomi, and retinervus Luffae fructus have synergistic effect in relieving pain. The traditional Chinese medicine formula for treating gout can quickly relieve pain and reduce swelling, is quick in effect taking, and is not easy to relapse.
2. In the traditional Chinese medicine formula for treating gout, effective components such as flavonoid and organic acid contained in sunflower discs, sunset abelmoschus flowers, acer truncatum oil, lemon powder, psyllium shells, kudzu roots, mulberry leaves, cinnamon, reed rhizome and loofah sponge in the traditional Chinese medicine formula for treating gout can inhibit the activity of XOD (xanthine oxidase) and promote the excretion of uric acid, so that the concentration of uric acid in serum is reduced, and the curative effect of treating gout is achieved; especially, when the sunflower disc, the abelmoschus manihot flower, the acer truncatum oil and the lemon powder are combined, a better effect can be achieved.
3. Researches prove that the problem of intestinal flora imbalance exists in patients with hyperuricemia and gout, the content of lactobacillus, bifidobacterium and clostridium flora with purine absorption and uric acid decomposition functions in the patients is reduced, the composite probiotics in the invention improves the intestinal bacteria imbalance condition in the patients with gout, and meanwhile, the acer truncatum oil also has the effects of adjusting the intestinal flora and repairing intestinal inflammatory injury; the synergistic effect of the composite probiotics and the traditional Chinese medicine mixture can further improve the treatment effect of gout.
Drawings
FIG. 1 is a flow chart of the preparation process of the traditional Chinese medicine formula for treating gout of the invention;
FIG. 2 is a comparison graph of the test of the swelling inhibition rate in the rat foot swelling test of the present invention;
FIG. 3 is a comparison of tests on volunteer pain and redness changes in the present invention;
figure 4 is a test chart comparing the number of relapses occurring within 6 months after two treatment sessions in accordance with the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without any creative work based on the embodiments of the present invention belong to the protection scope of the present invention.
Example 1
The embodiment discloses a preparation method of a traditional Chinese medicine mixture, which comprises the following steps:
(1) Washing radix Puerariae, rhizoma Phragmitis, opuntia ficus-indica, old rice, plantago ovata seed husk, lalang grass rhizome, cortex Cinnamomi, retinervus Luffae fructus, bulbus Lilii, endothelium corneum Gigeriae Galli, fructus Chaenomelis, folium Mori, sunflower disc, and flos Abelmoschi Manihot respectively with clear water, drying at constant temperature of 45 deg.C for 3h, sterilizing under ultraviolet lamp with power of 10w for 15min to obtain pretreated radix Puerariae, pretreated rhizoma Phragmitis, pretreated Opuntia ficus-indica, pretreated old rice, pretreated Plantago ovata seed husk, pretreated lalang grass rhizome, pretreated cortex Cinnamomi, pretreated retinervus Luffae fructus, pretreated Bulbus Lilii, pretreated endothelium corneum Gigeriae Galli, pretreated fructus Chaenomelis, pretreated folium Mori, pretreated sunflower disc, and pretreated flos Abelmoschi Manihot flower.
(2) Mixing 38 parts of pretreated radix puerariae, 18 parts of pretreated rhizoma phragmitis, 24 parts of pretreated pome cactus, 27 parts of pretreated rice in a warehouse, 18 parts of pretreated lalang grass rhizome, 8 parts of pretreated cinnamon, 8 parts of pretreated loofah sponge, 8 parts of pretreated lily, 6 parts of pretreated endothelium corneum gigeriae galli, 6 parts of pretreated pawpaw, 6 parts of pretreated mulberry leaf, 8 parts of pretreated sunflower disc and 8 parts of pretreated flower of abelmoschus manihot to obtain a pretreated traditional Chinese medicine mixture, adding the traditional Chinese medicine mixture into 10 times of water by mass, soaking for 40min, heating, continuously decocting for 30min after the water is boiled, and filtering to obtain a first filtrate; decocting the residue with 5 times of water for two times, decocting for 30min after the water is boiled, and filtering to obtain a second filtrate; mixing the second filtrate and the first filtrate uniformly, concentrating the traditional Chinese medicine liquid by vacuum rotary evaporation until the solid content is 28%, spray drying, controlling the air inlet temperature at 120 ℃, the air outlet temperature at 90 ℃, the rotation speed of an atomizer at 45r/min and the feeding speed at 3mL/min to obtain spray dried powder, crushing, and sieving with a 100-mesh sieve to obtain the traditional Chinese medicine extract powder.
(3) Taking 25 parts of lemon powder and 8 parts of acer truncatum buge oil for later use; taking 14 parts of pretreated psyllium husk, grinding, sieving with a 100-mesh sieve to obtain psyllium husk powder, and mixing the psyllium husk powder, lemon powder, chinese medicinal extract powder and acer truncatum oil to obtain a Chinese medicinal mixture.
Example 2
The embodiment discloses a preparation method of a traditional Chinese medicine mixture, which comprises the following steps:
(1) Washing radix Puerariae, rhizoma Phragmitis, opuntia ficus-indica, old rice, plantago ovata seed husk, lalang grass rhizome, cortex Cinnamomi, retinervus Luffae fructus, bulbus Lilii, endothelium corneum Gigeriae Galli, fructus Chaenomelis, folium Mori, sunflower disc, and flos Abelmoschi Manihot respectively with clear water, drying at constant temperature of 60 deg.C for 5h, sterilizing under ultraviolet lamp with power of 15w for 30min to obtain pretreated radix Puerariae, pretreated rhizoma Phragmitis, pretreated Opuntia ficus-indica, pretreated old rice, pretreated Plantago ovata seed husk, pretreated lalang grass rhizome, pretreated cortex Cinnamomi, pretreated retinervus Luffae fructus, pretreated Bulbus Lilii, pretreated endothelium corneum Gigeriae Galli, pretreated fructus Chaenomelis, pretreated folium Mori, pretreated sunflower disc, and pretreated flos Abelmoschi Manihot flower.
(2) Mixing 46 parts of pretreated radix puerariae, 26 parts of pretreated rhizoma phragmitis, 32 parts of pretreated pome cactus, 35 parts of pretreated rice in a storehouse, 26 parts of pretreated lalang grass rhizome, 16 parts of pretreated cinnamon, 16 parts of pretreated loofah sponge, 16 parts of pretreated lily, 14 parts of pretreated endothelium corneum gigeriae galli, 14 parts of pretreated pawpaw, 14 parts of pretreated mulberry leaf, 16 parts of pretreated sunflower disc and 16 parts of pretreated abelmoschus manihot to obtain a pretreated traditional Chinese medicine mixture, adding the traditional Chinese medicine mixture into 15 times of water by mass, soaking for 60min, heating, continuously decocting for 60min after the water is boiled, and filtering to obtain a first filtrate; decocting the residue with 10 times of water for two times, decocting for 45min after the water is boiled, and filtering to obtain a second filtrate; mixing the second filtrate and the first filtrate uniformly, concentrating the traditional Chinese medicine liquid by vacuum rotary evaporation until the solid content is 35%, spray drying, controlling the air inlet temperature at 150 ℃, the air outlet temperature at 100 ℃, the rotation speed of an atomizer at 50r/min, and the feeding speed at 6mL/min to obtain spray dried powder, crushing, and sieving with a 300-mesh sieve to obtain traditional Chinese medicine extract powder.
(3) Taking 45 parts of lemon powder and 16 parts of acer truncatum buge oil for later use; taking 22 parts of pretreated psyllium husk, grinding, sieving with a 300-mesh sieve to obtain psyllium husk powder, and mixing the psyllium husk powder, lemon powder, chinese medicinal extract powder and acer truncatum oil to obtain a Chinese medicinal mixture.
Example 3
The embodiment discloses a preparation method of a traditional Chinese medicine mixture, which comprises the following steps:
(1) Washing radix Puerariae, rhizoma Phragmitis, opuntia ficus-indica, chencang rice, plantago psyllium seed husk, lalang grass rhizome, cortex Cinnamomi, retinervus Luffae fructus, bulbus Lilii, endothelium corneum Gigeriae Galli, fructus Chaenomelis, folium Mori, sunflower disc, and flos Abelmoschi Manihot respectively with clear water, drying at constant temperature of 55 deg.C for 4h, sterilizing under ultraviolet lamp with power of 13w for 20min to obtain pretreated radix Puerariae, pretreated rhizoma Phragmitis, pretreated Opuntia ficus-indica, pretreated Chencang rice, pretreated Plantago psyllium seed husk, pretreated lalang grass rhizome, pretreated cortex Cinnamomi, pretreated retinervus Luffae fructus, pretreated Bulbus Lilii, pretreated endothelium corneum Gigeriae Galli, pretreated fructus Chaenomelis, pretreated folium Mori, pretreated sunflower disc, and pretreated flos Abelmoschi Manihot flower.
(2) Mixing 42 parts of pretreated radix puerariae, 22 parts of pretreated reed rhizome, 28 parts of pretreated opuntia ficus-indica, 31 parts of pretreated long-grain rice, 22 parts of pretreated lalang grass rhizome, 12 parts of pretreated cinnamon, 12 parts of pretreated loofah sponge, 12 parts of pretreated lily, 10 parts of pretreated endothelium corneum gigeriae galli, 10 parts of pretreated pawpaw, 10 parts of pretreated mulberry leaf, 12 parts of pretreated sunflower disc and 12 parts of pretreated sunset abelmoschus flower to obtain a pretreated traditional Chinese medicine mixture, adding the traditional Chinese medicine mixture into 13 times of water by mass, soaking for 50min, heating, continuing to decoct for 45min after the water is boiled, and filtering to obtain a first filtrate; decocting the residue with 8 times of water for two times, decocting for 40min after the water is boiled, and filtering to obtain a second filtrate; mixing the second filtrate and the first filtrate uniformly, concentrating the traditional Chinese medicine liquid by vacuum rotary evaporation until the solid content is 30%, spray drying, controlling the air inlet temperature at 135 ℃, the air outlet temperature at 95 ℃, the rotation speed of an atomizer at 48r/min and the feed speed at 4mL/min to obtain spray dried powder, crushing, and sieving with a 200-mesh sieve to obtain traditional Chinese medicine extract powder.
(3) Taking 35 parts of lemon powder and 12 parts of acer truncatum buge oil for later use; taking 18 parts of pretreated psyllium husk, grinding, sieving with a 200-mesh sieve to obtain psyllium husk powder, and mixing the psyllium husk powder, lemon powder, traditional Chinese medicine extract powder and acer truncatum oil to obtain a traditional Chinese medicine mixture.
Example 4
The embodiment discloses a preparation method of a composite probiotic seed liquid, which comprises the following steps:
inoculating lactobacillus plantarum from a solid culture medium by using an inoculating loop into an MRS broth culture medium, placing the MRS broth culture medium in a constant temperature oscillator at 35 ℃, and carrying out aerobic culture at 200rpm until the culture reaches a stationary phase to obtain a seed solution of the lactobacillus plantarum; inoculating Bifidobacterium bifidum and Clostridium butyricum into MRS and RCM liquid culture medium respectively, and performing anaerobic culture at 35 deg.C to stabilization phase to obtain seed solutions of Bifidobacterium bifidum and Clostridium butyricum respectively; mixing the lactobacillus plantarum, the bifidobacterium bifidum and the clostridium butyricum seed liquid according to the volume ratio of 2.
Example 5
The embodiment discloses a preparation method of composite probiotic seed liquid, which comprises the following steps:
inoculating lactobacillus plantarum from a solid culture medium by using an inoculating loop into an MRS broth culture medium, placing the MRS broth culture medium in a constant temperature oscillator at 36 ℃, and carrying out aerobic culture at 210rpm until the culture reaches a stationary phase to obtain a seed solution of the lactobacillus plantarum; inoculating Bifidobacterium bifidum and Clostridium butyricum into MRS and RCM liquid culture medium respectively, and anaerobically culturing at 36 deg.C to stationary phase to obtain seed solutions of Bifidobacterium bifidum and Clostridium butyricum respectively; mixing the lactobacillus plantarum, the bifidobacterium bifidum and the clostridium butyricum seed liquid according to the volume ratio of 3.
Example 6
The embodiment discloses a preparation method of a composite probiotic seed liquid, which comprises the following steps:
inoculating lactobacillus plantarum from a solid culture medium by using an inoculating loop into an MRS broth culture medium, placing the MRS broth culture medium in a constant temperature oscillator at 37 ℃, and carrying out aerobic culture at 220rpm until the culture reaches a stationary phase to obtain a seed solution of the lactobacillus plantarum; inoculating Bifidobacterium bifidum and Clostridium butyricum into MRS and RCM liquid culture medium respectively, and performing anaerobic culture at 37 deg.C to stationary phase to obtain seed solutions of Bifidobacterium bifidum and Clostridium butyricum respectively; mixing the lactobacillus plantarum, the bifidobacterium bifidum and the clostridium butyricum seed liquid according to a volume ratio of 4.
Example 7
The embodiment discloses a preparation method of a traditional Chinese medicine mixture, which comprises the following steps:
adding water which is 1 time of the total mass of the traditional Chinese medicine mixture, a nutrient substrate which is 5% of the total mass of the traditional Chinese medicine mixture and the water mixture (the mass ratio of corn starch to brown sugar to fructo-oligosaccharide to lysine to talcum powder is 2; and (3) inoculating the composite probiotic seed liquid accounting for 20% of the total mass of the fermentation substrate after cooling, performing closed fermentation at 35 ℃ for 48h, air-drying and crushing, and sieving by a 60-mesh sieve to obtain the traditional Chinese medicine formula for treating gout.
Example 8
The embodiment discloses a preparation method of a traditional Chinese medicine mixture, which comprises the following steps:
adding water in an amount which is 2 times of the total mass of the traditional Chinese medicine mixture into the traditional Chinese medicine mixture, and adding a nutrient substrate (the mass ratio of corn starch to brown sugar to fructo-oligosaccharide to lysine to talcum powder is 4; and (3) inoculating the composite probiotic seed liquid accounting for 40% of the total mass of the fermentation substrate after cooling, fermenting for 60 hours in a closed manner at 37 ℃, air-drying and crushing, and sieving by a 100-mesh sieve to obtain the traditional Chinese medicine formula for treating gout.
Example 9
The embodiment discloses a preparation method of a traditional Chinese medicine mixture, which comprises the following steps:
adding water which is 1.4 times of the total mass of the traditional Chinese medicine mixture and a nutrient substrate which is 10% of the total mass of the traditional Chinese medicine mixture and the water mixture (the mass ratio of corn starch to brown sugar to fructo-oligosaccharide to lysine to talcum powder is 2.5; and (3) inoculating composite probiotic seed liquid accounting for 25% of the total mass of the fermentation substrate after cooling, hermetically fermenting for 50h at 35 ℃, air-drying and crushing, and sieving with a 80-mesh sieve to obtain the traditional Chinese medicine formula for treating gout.
Example 10
The embodiment discloses a preparation method of a traditional Chinese medicine mixture, which comprises the following steps:
adding water which is 1.8 times of the total mass of the traditional Chinese medicine mixture and a nutrient substrate which is 20% of the total mass of the traditional Chinese medicine mixture and the water mixture (the mass ratio of the corn starch to the brown sugar to the fructo-oligosaccharide to the lysine to the talcum powder is 3; and (3) inoculating the composite probiotic seed liquid accounting for 30% of the total mass of the fermentation substrate after cooling, fermenting for 55 hours in a closed manner at 36 ℃, air-drying and crushing, and sieving by a 80-mesh sieve to obtain the traditional Chinese medicine formula for treating gout.
Example 11
The embodiment discloses a preparation method of a traditional Chinese medicine mixture, which comprises the following steps:
adding water which is 1.9 times of the total mass of the traditional Chinese medicine mixture into the traditional Chinese medicine mixture, and a nutrient substrate (the mass ratio of corn starch to brown sugar to fructo-oligosaccharide to lysine to talcum powder is 3.5; and (3) inoculating composite probiotic seed liquid accounting for 35% of the total mass of the fermentation substrate after cooling, fermenting in a closed manner at 36 ℃ for 55h, air-drying and crushing, and sieving by a 90-mesh sieve to obtain the traditional Chinese medicine formula for treating gout.
The traditional Chinese medicine mixture prepared in the example 3 is selected as the traditional Chinese medicine mixture in the examples 7 to 11, and the composite probiotic seed liquid prepared in the example 5 is selected as the composite probiotic seed liquid.
Comparative example 1
Compared with example 9, the traditional Chinese medicine mixture adopted in comparative example 1 is not added with radix puerariae, and other conditions are not changed, so that a mixed solution comparative sample 1 is obtained.
Comparative example 2
Compared with example 9, the traditional Chinese medicine mixture adopted in comparative example 2 is not added with cinnamon, and other conditions are not changed.
Comparative example 3
Compared with example 9, the traditional Chinese medicine mixture adopted in comparative example 3 is not added with loofah sponge, and other conditions are not changed.
Comparative example 4
Compared with example 8, the traditional Chinese medicine mixture adopted in comparative example 4 is not added with reed rhizome, and other conditions are not changed.
Comparative example 5
Compared with example 9, the traditional Chinese medicine mixture adopted in comparative example 5 is not added with Opuntia ficus-indica, and other conditions are not changed.
Comparative example 6
Compared with example 9, the traditional Chinese medicine formula for treating gout in comparative example 5 is not added with the composite probiotic seed liquid, and other conditions are not changed.
Experiment one: swelling experiment of rat feet
Taking 120 healthy Wistar rats which are male and randomly divided into 12 groups, injecting microcrystalline sodium urate into all the rats to induce the swelling of the feet of the rats, measuring the circumferences of the same parts of the anklebones of the rats after 1h of inflammation, recording the circumferences as the circumferences of the blank feet, soaking the formulas for treating gout, prepared in examples 7-11 and comparative examples 1-6, in boiled water (200 mL of boiled water is used for soaking every 5g of the formula) to obtain samples 1-5 and comparative samples 1-6, then performing clysis on the mice by adopting 20mL of the corresponding samples of each group and 20mL of physiological saline with the concentration of 0.9% (a control group), measuring the circumferences of the same parts of the anklebones of the rats again after 6h of inflammation, recording the circumferences as the circumferences of the feet of the traditional Chinese medicine samples, and calculating the swelling inhibition rate: one set for each sample, and one control set.
Swelling inhibition (%) = (circumference of empty paw-circumference of traditional Chinese medicine sample paw) ÷ circumference of empty paw; the test results are shown in table 1:
TABLE 1
Swelling inhibition ratio (%) Swelling inhibition ratio (%)
Sample 1 65.82 Comparative sample 1 62.17
Sample 2 69.39 Comparative sample 2 63.04
Sample 3 66.61 Comparative sample 3 62.33
Sample No. 4 66.97 Comparative sample 4 42.11
Sample No. 5 69.27 Comparative sample 5 38.96
Control group 0.78 Comparative sample 6 62.09
As can be seen from the test results of table 1: the sample group and the comparative sample group have significant differences compared with the control group. From the results of comparative samples 4 to 5, it can be seen that: in the absence of either reed rhizome or opuntia ficus-indica, the swelling inhibition effect is greatly reduced, so that the two have synergistic effect in inhibiting swelling.
Experiment II, influence of traditional Chinese medicine formula for treating gout on condition of gout patient
The traditional Chinese medicine formula for treating gout prepared in examples 7-11 and comparative examples 1-6 is prepared into solid beverages, wherein 5g of the solid beverages are packaged in one bag, the corresponding numbers are 1-5 samples and 1-6 comparative samples, 110 volunteers with gout diseases are selected, 10 volunteers are taken as one group, one bag is taken every day, 30 days are taken as one treatment course, and the solid beverages are continuously taken for two treatment courses.
In the experimental process, the days for the volunteers to start to disappear the pain, the days for the red and swollen volunteers to disappear and the number of the volunteers to relapse within 6 months after the two treatment courses are finished are recorded; the test results are shown in table 2:
TABLE 2
Figure BDA0003850209740000181
As can be seen from the test results of table 2: the formula of the invention has quick effect and no recurrence, and can achieve the effect of radical treatment. From comparative samples 1 to 3, it can be seen that: the days that the kudzu root, the cinnamon and the loofah sponge lack any one pain relieving function can be prolonged, the kudzu root, the cinnamon and the loofah sponge have synergistic effect on pain relieving, and the comparison samples 4-5 show that: the lack of reed rhizome or the prolonged time for the detumescence of either Opuntia Ficus lndica, it is known that reed rhizome and Opuntia Ficus lndica have a synergistic effect on the detumescence.
Third, detecting the uric acid content in serum
120 healthy Wistar rats with the body weight of 180 +/-20 g are taken and divided into 12 groups at random, wherein the 12 groups are respectively a normal group (normal saline is given), a sample group 1-5 (the cooling liquid medicine obtained by soaking the traditional Chinese medicine formula obtained in examples 7-11 in warm boiled water), a comparative sample group 1-6 (the cooling liquid medicine obtained by soaking the traditional Chinese medicine formula obtained in comparative examples 1-6 in warm boiled water) and a model group.
The molding method and the administration method comprise the following steps: after one week of acclimatization of rats, the model was replicated for 21 days by intraperitoneal injection of hypoxanthine (0.125 g/kg) and subcutaneous injection of Potassium Oxonate (0.05 g/kg) into the neck daily. The liquid medicine after the stomach irrigation and cooling is performed on the sample group and the comparative sample group 1 time every morning, 4mL (200 mL of boiled water is used for soaking every 5g of medicinal tea) is performed each time, and the equal volume of physiological saline (15 mL/kg) is continuously performed for 21 days in the blank group and the model group after the stomach irrigation. After the model is copied on the 21 st day, the rat is anesthetized by using 20% urethane solution through intraperitoneal injection within 1h, blood is taken from abdominal aorta, and the content of uric acid in the blood of the rat is measured.
The data are all "mean. + -. Standard deviation"
Figure BDA0003850209740000191
Data were processed using statistical software SPSS17.0 and significance of differences was analyzed using one-way anova.
The test results are shown in table 3:
TABLE 3 uric acid content in mouse serum: (
Figure BDA0003850209740000192
n＝10)
Uric acid/(mu mol/L) Uric acid/(mu mol/L)
Sample 1 213.19±37.00 Comparative sample 1 221.62±42.84
Sample 2 185.47±46.86 Comparative sample 2 219.76±42.55
Sample 3 210.36±39.18 Comparative sample 3 223.16±37.00
Sample No. 4 204.36±39.18 Comparative sample 4 225.76±42.34
Sample No. 5 197.36±39.18 Comparative sample 5 215.02±43.51
Normal group 153.49±55.76 Comparative sample 6 229.58±39.73
Model set 650.11±109.97
Serum uric acid level is directly related to gout, hyperuricemia is an important risk factor formed by gout and the pathological basis of gout, and as can be seen from table 3, the traditional Chinese medicine formula for treating gout has a significant uric acid reducing effect, so that the traditional Chinese medicine formula also has a great positive effect on the treatment of gout. As can be seen from the comparative samples 1-6, the kudzu root, the cinnamon, the loofah sponge, the reed rhizome and the composite probiotic seed liquid have different control effects on the uric acid content in the serum of the mouse.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. The traditional Chinese medicine formula for treating gout is characterized by comprising a traditional Chinese medicine mixture and composite probiotics; the traditional Chinese medicine extract powder comprises: radix Puerariae, rhizoma Phragmitis, fructus Pyri Opuntiae Dillenii, old rice, plantago ovata forsk shell, lalang grass rhizome, cortex Cinnamomi, retinervus Luffae fructus, bulbus Lilii, endothelium corneum Gigeriae Galli, fructus Chaenomelis, folium Mori, sunflower disc, flos Abelmoschi Manihot, acer Truncatum Bunge oil, and fructus Citri Limoniae powder; the composite probiotics comprise lactobacillus plantarum, bifidobacterium bifidum and clostridium butyricum.
2. The traditional Chinese medicine formula for treating gout according to claim 1, wherein the preparation method of the traditional Chinese medicine mixture comprises the following steps:
(1) Cleaning radix Puerariae, rhizoma Phragmitis, opuntia ficus-indica, chencang rice, plantago depressa seed husk, lalang grass rhizome, cortex Cinnamomi, retinervus Luffae fructus, bulbus Lilii, endothelium corneum Gigeriae Galli, fructus Chaenomelis, folium Mori, helianthus annuus disc, and flos Abelmoschi Manihot, drying at constant temperature, and sterilizing to obtain pretreated radix Puerariae, pretreated rhizoma Phragmitis, pretreated Opuntia ficus-indica, pretreated Chencang rice, pretreated Plantago depressa seed husk, pretreated lalang grass rhizome, pretreated cortex Cinnamomi, pretreated retinervus Luffae fructus, pretreated Bulbus Lilii, pretreated endothelium corneum Gigeriae Galli, pretreated fructus Chaenomelis, pretreated folium Mori, pretreated Helianthus annuus disc, and pretreated flos Abelmoschi Manihot flower;
(2) Mixing 38-46 parts of pretreated kudzu root, 18-26 parts of pretreated reed rhizome, 24-32 parts of pretreated pear cactus, 27-35 parts of pretreated long-stalked rice, 18-26 parts of pretreated lalang grass rhizome, 8-16 parts of pretreated cinnamon, 8-16 parts of pretreated loofah sponge, 8-16 parts of pretreated lily, 6-14 parts of pretreated chicken gizzard membrane, 6-14 parts of pretreated pawpaw, 6-14 parts of pretreated mulberry leaf, 8-16 parts of pretreated sunflower disc and 8-16 parts of pretreated sunset abelmoschus flower to obtain a pretreated traditional Chinese medicine mixture, adding the traditional Chinese medicine mixture into water, soaking, heating, continuously decocting after water is boiled, and filtering to obtain a first filtrate; decocting the residue with water for the second time, boiling, decocting, and filtering to obtain second filtrate; mixing the second filtrate and the first filtrate, vacuum rotary evaporating to concentrate the Chinese medicinal liquid until the solid content is above 25%, spray drying, pulverizing, and sieving to obtain Chinese medicinal extract powder.
(3) Taking 25-45 parts of lemon powder and 8-16 parts of acer truncatum oil for later use; taking 14-22 parts of pretreated Plantago ovata forsk, grinding, sieving to obtain Plantago ovata forsk powder, and mixing Plantago ovata forsk powder, lemon powder, chinese medicinal extract powder and Acer truncatum oil to obtain a Chinese medicinal mixture.
3. The method for preparing a Chinese medicinal mixture according to claim 2, wherein the conditions of constant temperature drying are as follows: drying at constant temperature of 45-60 ℃ for 3-5h, and sterilizing: sterilizing under ultraviolet lamp with power of 10-15w for 15-30min.
4. The method for preparing a Chinese medicinal mixture according to claim 2, wherein the preparation process of the first filtrate comprises the following steps: the mass ratio of the traditional Chinese medicine mixture to the water is as follows: 1, 10-15, soaking time: 40-60min, decocting time: 30-60min; in the preparation process of the second filtrate: the mass ratio of the filter residue to the water is as follows: 1:5-10, decoction time: 30-45min.
5. The method for preparing a Chinese medicinal mixture according to claim 2, wherein the spray drying conditions are as follows: the air inlet temperature is controlled to be 120-150 ℃, the air outlet temperature is controlled to be 90-100 ℃, the rotation speed of the atomizer is 45-50r/min, and the feeding speed is 3-6mL/min.
6. The traditional Chinese medicine formula for treating gout according to claim 1, wherein the culture method of the composite probiotics comprises the following steps:
inoculating lactobacillus plantarum from a solid culture medium by using an inoculating loop into an MRS broth culture medium, placing the MRS broth culture medium in a constant temperature oscillator at 35-37 ℃, and carrying out aerobic culture at 200-220rpm until the culture reaches a stationary phase to obtain a seed solution of the lactobacillus plantarum; inoculating Bifidobacterium bifidum and Clostridium butyricum into MRS and RCM liquid culture medium respectively, and anaerobically culturing at 35-37 deg.C to stationary phase to obtain seed solutions of Bifidobacterium bifidum and Clostridium butyricum respectively; mixing the seed liquid of lactobacillus plantarum, bifidobacterium bifidum and clostridium butyricum according to the volume ratio of 2-4:2-4:1-3 to obtain the composite probiotic seed liquid.
7. The traditional Chinese medicine formula for treating gout according to claim 1, wherein the preparation method of the traditional Chinese medicine formula for treating gout comprises the following steps: adding 1-2 times of water and 5-30% of nutrient substrate as fermentation substrate into the Chinese medicinal mixture, mixing, and autoclaving at 115-125 deg.C for 15-30min; and (3) inoculating the composite probiotic seed liquid accounting for 20-40% of the total mass of the fermentation substrate after cooling, fermenting for 48-60h in a closed manner at 35-37 ℃, air-drying and crushing, and sieving with a 60-100-mesh sieve to obtain the traditional Chinese medicine formula for treating gout.
8. The method for preparing a traditional Chinese medicine formula for treating gout according to claim 7, wherein the nutritional substrate comprises the following components in percentage by mass: 1-2 parts of corn starch, brown sugar, fructo-oligosaccharide, lysine and talcum powder, wherein the corn starch is 2-4:2-4:2-3; the number of viable bacteria contained in the traditional Chinese medicine formula for treating gout is not less than 1 multiplied by 10 9 CFU/g。
9. The traditional Chinese medicine formula for treating gout according to claim 1, wherein the traditional Chinese medicine formula for treating gout is prepared into pharmaceutically acceptable capsules, granules, powder, pills, tablets and oral liquid preparations by the prior art.
10. A traditional Chinese medicine formulation for treating gout prepared according to the method of claims 1-9.
CN202211133731.4A 2022-09-16 2022-09-16 Traditional Chinese medicine formula for treating gout and preparation method thereof Pending CN115518130A (en)

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