CN115517368A - Whey protein powder for recovering functions after exercise and preparation method thereof - Google Patents
Whey protein powder for recovering functions after exercise and preparation method thereof Download PDFInfo
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- CN115517368A CN115517368A CN202211127337.XA CN202211127337A CN115517368A CN 115517368 A CN115517368 A CN 115517368A CN 202211127337 A CN202211127337 A CN 202211127337A CN 115517368 A CN115517368 A CN 115517368A
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- 238000002360 preparation method Methods 0.000 title claims description 12
- 108010067454 caseinomacropeptide Proteins 0.000 claims abstract description 46
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/19—Dairy proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Water Supply & Treatment (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses whey protein powder for recovering functions after exercise, which consists of the following components: casein glycomacropeptide and whey protein powder; the mass ratio of the casein glycomacropeptide to the whey protein powder is 1: (3-6). The whey protein powder disclosed by the invention hardly contains lactose and fat, and is rich in essential amino acid. As the optimal protein supplement for the diabetic, the SP-9225 concentrated whey protein powder effectively improves the sensitivity of muscles to insulin, effectively improves the glutamine level of a human body, supports the synthesis of muscle protein, repairs muscle damage after exercise, promotes the recovery after exercise, reduces the muscle loss, reduces the bone loss of the old and reduces the occurrence of osteoporosis, and simultaneously can provide an energy substance basis in the treatment process of the cancer patient, is a high-quality nutrition supplement source for the cancer patient and is the only food for the lactose intolerant patient.
Description
Technical Field
The invention relates to the technical field of food, in particular to whey protein powder for recovering functions after exercise and a preparation method thereof.
Background
Increasing muscle can help to accelerate metabolism, reduce body fat content, reduce cholesterol and blood pressure, and is beneficial for weight loss and cardiovascular health maintenance. Can also improve the utilization rate of glucose by human body, thereby preventing and treating diabetes. The increase of muscle can also increase bone density, and has important significance for preventing osteoporosis. Therefore, it is desirable from both aesthetic and health perspectives, especially for athletes and fitness people.
The whey protein has the advantages of high purity, high absorption rate, most reasonable amino acid composition and the like, is easily absorbed and utilized by human bodies, and is very suitable for body-building and body-building enthusiasts, athletes and the like. Pure whey protein powder can rapidly supply nutrition, but the effect that the body can be rapidly recovered after exercise is insufficient, so that the research on whey protein powder for recovering the function after exercise and a preparation method thereof are necessary.
Disclosure of Invention
Based on the technical problems in the background art, the invention provides whey protein powder for recovering functions after exercise and a preparation method thereof.
The technical scheme of the invention is as follows:
whey protein powder for recovering functions after exercise comprises the following components: casein Glycomacropeptide (CGMP) and whey protein powder (SP-9225).
Preferably, in the whey protein powder for recovering functions after exercise, the mass ratio of the casein glycomacropeptide to the whey protein powder is 1: (3-6).
Preferably, the whey protein powder (SP-9225) has a protein content of more than or equal to 92.25%.
A preparation method of whey protein powder for recovering functions after exercise comprises the following steps:
A. collecting fresh whey, filtering with mesh screen and gauze, separating impurities, and collecting supernatant;
B. adding 1.2-1.5mol/L calcium chloride 5-10ml/L into the supernatant, adjusting pH to 4.5-7.5, stirring in 55-65 deg.C water bath for 15-20min, clarifying cheese whey by flocculation centrifugation, microfiltration or flocculation microfiltration to obtain whey protein solution;
C. concentrating the clarified whey protein liquid by using a regenerated cellulose ultrafiltration membrane with the molecular weight cutoff of 15-20KDa under the operating conditions of 25-30 ℃, 0.1-0.2MPa of pressure and 25-40m/s of flow rate, wherein the concentration times are 5-10 times to obtain a first whey protein concentrated liquid;
D. adding deionized water into the whey protein concentrated solution, wherein the material-liquid ratio is 6-10:1, the flow rate is 25-40m/s, the pressure is 0.1-0.2MPa, performing secondary concentration by using a regenerated cellulose ultrafiltration membrane with the molecular weight cutoff of 5-10KDa, and removing lactose to obtain a purified whey protein concentrated solution;
E. freeze drying or spray drying the purified whey protein concentrate in a pressure type spray tower;
F. dissolving casein glycomacropeptide in water, and performing ultrasonic dispersion to obtain a casein glycomacropeptide solution;
G. under the action of hot air from bottom to top, the whey protein powder is in a flowing state, and casein glycomacropeptide solution is sprayed in a spraying mode at the same time, and the molding and drying are carried out simultaneously.
Preferably, in the step E, the spray drying conditions are: the temperature of a sample inlet is 115-135 ℃, the adjusting range of the amount of dry air is 0.50-0.80 cubic meter/min, the spraying pressure is 16 multiplied by 10kPa, the flow of a liquid delivery pump is 1.5-2.5mL/min, the rotating speed of a stirrer is 350-450rpm, and the whey protein powder can be obtained by sieving through a 100-200 mesh sieve.
Preferably, in step F, the mass concentration of the casein glycomacropeptide solution is 1.5-2.5%.
Preferably, in the step G, the spraying speed is 15-25kg/h.
The invention has the advantages that: the whey protein powder for recovering the function after exercise consists of the following components: casein glycomacropeptide and whey protein powder; the mass ratio of the casein glycomacropeptide to the whey protein powder is 1: (3-6). The whey protein powder of the invention contains almost no lactose and fat, and is rich in essential amino acid. As the optimal protein supplement for the diabetic, the SP-9225 concentrated whey protein powder effectively improves the sensitivity of muscles to insulin, effectively improves the glutamine level of a human body, supports the synthesis of muscle protein, repairs muscle damage after exercise, promotes the recovery after exercise, reduces the muscle loss, reduces the bone loss of the old and reduces the occurrence of osteoporosis, and simultaneously can provide an energy substance basis in the treatment process of the cancer patient, is a high-quality nutrition supplement source for the cancer patient and is the only food for the lactose intolerant patient.
Detailed Description
Example 1
Whey protein powder for recovering functions after exercise comprises the following components: casein Glycomacropeptide (CGMP) and whey protein powder (SP-9225); the mass ratio of the casein glycomacropeptide to the whey protein powder is 1:4.
in the whey protein powder (SP-9225), the protein content is more than or equal to 92.25 percent.
A preparation method of whey protein powder for recovering functions after exercise comprises the following steps:
A. collecting fresh whey, filtering with mesh screen and gauze, separating impurities, and collecting supernatant;
B. adding 1.3mol/L calcium chloride 8ml/L into the supernatant, adjusting pH to 6.5, stirring in water bath at 60 deg.C for 18min, clarifying the cheese whey by flocculation centrifugation, microfiltration or flocculation microfiltration to obtain whey protein solution;
C. concentrating the clarified whey protein liquid by adopting a regenerated cellulose ultrafiltration membrane with the molecular weight cutoff of 18KDa under the operating conditions of 28 ℃, 0.15MPa of pressure, 35m/s of flow rate and concentration multiple of 8 times to obtain a first whey protein concentrated solution;
D. adding deionized water into the whey protein concentrated solution, wherein the material-liquid ratio is 7:1, the flow rate is 35m/s, the pressure is 0.15MPa, and performing secondary concentration by using a regenerated cellulose ultrafiltration membrane with the molecular weight cutoff of 7KDa to remove lactose to obtain a purified whey protein concentrated solution;
E. freeze drying or spray drying the purified whey protein concentrate in a pressure type spray tower;
F. dissolving casein glycomacropeptide in water, and performing ultrasonic dispersion to obtain a casein glycomacropeptide solution;
G. under the action of hot air from bottom to top, the whey protein powder is in a flowing state, and casein glycomacropeptide solution is sprayed in a spraying mode at the same time, and the molding and drying are carried out simultaneously.
In the step E, the spray drying conditions are as follows: the temperature of a sample inlet is 125 ℃, the adjusting range of the amount of dry air is 0.75 cubic meter/min, the spraying pressure is 16 multiplied by 10kPa, the flow of a liquid delivery pump is 2.2L/min, the rotating speed of a stirrer is 400rpm, and the whey protein powder can be obtained by sieving through a 120-mesh sieve.
In the step F, the mass concentration of the casein glycomacropeptide solution is 1.8%.
In the step G, the spraying speed is 22kg/h.
Example 2
Whey protein powder for recovering functions after exercise comprises the following components: casein Glycomacropeptide (CGMP) and whey protein powder (SP-9225); the mass ratio of the casein glycomacropeptide to the whey protein powder is 1:6.
in the whey protein powder (SP-9225), the protein content is more than or equal to 92.25 percent.
A preparation method of whey protein powder for recovering functions after exercise comprises the following steps:
A. collecting fresh whey, filtering with mesh screen and gauze, separating impurities, and collecting supernatant;
B. adding 1.2mol/L calcium chloride 5-10ml/L into the supernatant, adjusting pH to 7.5, stirring in water bath at 55 deg.C for 20min, clarifying cheese whey by flocculation centrifugation, microfiltration or flocculation microfiltration to obtain whey protein solution;
C. concentrating the clarified whey protein liquid by adopting a regenerated cellulose ultrafiltration membrane with the molecular weight cutoff of 15KDa under the operating conditions of 30 ℃, 0.1MPa of pressure, 40m/s of flow rate and concentration multiple of 5 times to obtain a first whey protein concentrated solution;
D. adding deionized water into the whey protein concentrated solution, wherein the material-liquid ratio is 10, the flow rate is 25m/s, the pressure is 0.2MPa, and performing secondary concentration by adopting a regenerated cellulose ultrafiltration membrane with the molecular weight cutoff of 5KDa to remove lactose to obtain a purified whey protein concentrated solution;
E. freeze drying or spray drying the purified whey protein concentrate by a pressure type spray tower;
F. dissolving casein glycomacropeptide in water, and performing ultrasonic dispersion to obtain a casein glycomacropeptide solution;
G. under the action of hot air from bottom to top, the whey protein powder is in a flowing state, and casein glycomacropeptide solution is sprayed in a spraying mode, and the molding and drying are carried out simultaneously.
In the step E, the spray drying conditions are as follows: the temperature of a sample inlet is 135 ℃, the adjusting range of the amount of dry air is 0.50 cubic meter/min, the spraying pressurization is 16 multiplied by 10kPa, the flow rate of a liquid transfer pump is 2.5mL/min, the rotating speed of a stirrer is 350rpm, and the whey protein powder can be obtained by sieving with a 200-mesh sieve.
In the step F, the mass concentration of the casein glycomacropeptide solution is 1.5%.
In the step G, the spraying speed is 25kg/h.
Example 3
Whey protein powder for recovering functions after exercise comprises the following components: casein Glycomacropeptide (CGMP) and whey protein powder (SP-9225); the mass ratio of the casein glycomacropeptide to the whey protein powder is 1:3.
in the whey protein powder (SP-9225), the protein content is more than or equal to 92.25 percent.
A preparation method of whey protein powder for recovering functions after exercise comprises the following steps:
A. collecting fresh whey, filtering with mesh screen and gauze, separating impurities, and collecting supernatant;
B. adding 1.5mol/L calcium chloride 5ml/L into the supernatant, adjusting pH to 7.5, stirring in water bath at 65 deg.C for 15min, and clarifying cheese whey by flocculation centrifugation, microfiltration or flocculation microfiltration to obtain whey protein solution;
C. concentrating the clarified whey protein liquid by using a regenerated cellulose ultrafiltration membrane with the molecular weight cutoff of 20KDa under the operating conditions of 25 ℃, 0.2MPa of pressure, 25m/s of flow rate and 10 times of concentration times to obtain a first whey protein concentrated solution;
D. adding deionized water into the whey protein concentrated solution, wherein the material-liquid ratio is 6:1, the flow rate is 40m/s, the pressure is 0.1MPa, performing secondary concentration by adopting a regenerated cellulose ultrafiltration membrane with the molecular weight cutoff of 10KDa, and removing lactose to obtain a purified whey protein concentrated solution;
E. freeze drying or spray drying the purified whey protein concentrate in a pressure type spray tower;
F. dissolving casein glycomacropeptide in water, and performing ultrasonic dispersion to obtain a casein glycomacropeptide solution;
G. under the action of hot air from bottom to top, the whey protein powder is in a flowing state, and casein glycomacropeptide solution is sprayed in a spraying mode at the same time, and the molding and drying are carried out simultaneously.
In the step E, the spray drying conditions are as follows: the temperature of a sample inlet is 115 ℃, the adjusting range of the amount of dry air is 0.80 cubic meter/min, the spraying pressure is 16 multiplied by 10kPa, the flow of a liquid transfer pump is 1.5mL/min, the rotating speed of a stirrer is 450rpm, and the whey protein powder can be obtained by sieving with a 100-mesh sieve.
In the step F, the mass concentration of the casein glycomacropeptide solution is 2.5%.
In the step G, the spraying speed is 15kg/h.
Example 4
Whey protein powder for recovering functions after exercise comprises the following components: casein Glycomacropeptide (CGMP) and whey protein powder (SP-9225); the mass ratio of the casein glycomacropeptide to the whey protein powder is 1:4.5.
in the whey protein powder (SP-9225), the protein content is more than or equal to 92.25 percent.
A preparation method of whey protein powder for recovering functions after exercise comprises the following steps:
A. collecting fresh whey, filtering with mesh screen and gauze, separating impurities, and collecting supernatant;
B. adding 1.3mol/L calcium chloride 5ml/L into the supernatant, adjusting pH to 5.0, stirring in water bath at 65 deg.C for 17min, and clarifying cheese whey by flocculation centrifugation, microfiltration or flocculation microfiltration to obtain whey protein solution;
C. concentrating the clarified whey protein liquid by adopting a regenerated cellulose ultrafiltration membrane with the molecular weight cutoff of 18KDa under the operating conditions of 27 ℃, 0.15MPa of pressure, 35m/s of flow rate and concentration multiple of 5 times to obtain a first whey protein concentrated solution;
D. adding deionized water into the whey protein concentrated solution, wherein the material-liquid ratio is 8;
E. freeze drying or spray drying the purified whey protein concentrate by a pressure type spray tower;
F. dissolving casein glycomacropeptide in water, and performing ultrasonic dispersion to obtain a casein glycomacropeptide solution;
G. under the action of hot air from bottom to top, the whey protein powder is in a flowing state, and casein glycomacropeptide solution is sprayed in a spraying mode at the same time, and the molding and drying are carried out simultaneously.
In the step E, the spray drying conditions are as follows: the temperature of a sample inlet is 125 ℃, the adjusting range of the amount of dry air is 0.72 cubic meter/min, the spraying pressure is 16 multiplied by 10kPa, the flow of a liquid transfer pump is 1.8mL/min, the rotating speed of a stirrer is 450rpm, and the whey protein powder can be obtained by sieving through a 120-mesh sieve.
In the step F, the mass concentration of the casein glycomacropeptide solution is 1.8%.
In the step G, the spraying speed is 20kg/h.
Example 5
Whey protein powder for recovering functions after exercise comprises the following components: casein Glycomacropeptide (CGMP) and whey protein powder (SP-9225); the mass ratio of the casein glycomacropeptide to the whey protein powder is 1:5.
in the whey protein powder (SP-9225), the protein content is more than or equal to 92.25 percent.
A preparation method of whey protein powder for recovering functions after exercise comprises the following steps:
A. collecting fresh whey, filtering with mesh screen and gauze, separating impurities, and collecting supernatant;
B. adding 8ml/L of 1.3mol/L calcium chloride into the supernatant, adjusting the pH to 5.8, stirring in a water bath at the temperature of 58 ℃ for 18min, and clarifying the cheese whey by adopting a flocculation centrifugal method, a microfiltration method or a flocculation microfiltration method to obtain whey protein liquid for later use;
C. concentrating the clarified whey protein liquid by adopting a regenerated cellulose ultrafiltration membrane with the molecular weight cutoff of 16KDa under the operating conditions of 27 ℃, 0.15MPa of pressure and 35m/s of flow rate, wherein the concentration multiple is 9 times to obtain a first whey protein concentrated solution;
D. adding deionized water into the whey protein concentrated solution, wherein the material-liquid ratio is 8;
E. freeze drying or spray drying the purified whey protein concentrate by a pressure type spray tower;
F. dissolving casein glycomacropeptide in water, and performing ultrasonic dispersion to obtain a casein glycomacropeptide solution;
G. under the action of hot air from bottom to top, the whey protein powder is in a flowing state, and casein glycomacropeptide solution is sprayed in a spraying mode, and the molding and drying are carried out simultaneously.
In the step E, the spray drying conditions are as follows: the temperature of a sample inlet is 125 ℃, the adjusting range of the amount of dry air is 0.7 cubic meter/min, the spraying pressure is 16 multiplied by 10kPa, the flow of an infusion pump is 1.7mL/min, the rotating speed of a stirrer is 350rpm, and the whey protein powder can be obtained by sieving with a 200-mesh sieve.
In the step F, the mass concentration of the casein glycomacropeptide solution is 1.6%.
In the step G, the spraying speed is 22kg/h.
The whey protein powder was subjected to the following anti-fatigue effect test on mice by the institute of nutrition and food health of the national academy of preventive medicine sciences to obtain the following test results.
Animal treatment: the male mice weigh 18-22g, the mice are divided into four groups A, B, C and D, 50 mice in each group are divided into five groups, each group comprises 10 mice, namely a blank control group (physiological saline), and the whey protein powder is administered in the dose of 100mg/kg. W in example 1, example 2 and example 3; the positive drug control group is given with rhodiola rosea tablets, and the Chinese drug standard B20050013 dose is 100mg/kg.w. The control group was administered with physiological saline, weighed twice a week, and the amount of the test sample per mouse was adjusted by body weight for 30 consecutive days.
Test method
Experiment 1: experiment of oxygen deficiency resistance under normal pressure
Each group of 10 mice was placed in 250mL ground bottles (1 mouse per bottle) containing 5g soda lime 1 hour after the last gastric feeding, the bottle mouth was sealed with vaseline and tightly closed to prevent air leakage, the time was immediately counted, and the death time of the mice was recorded by taking the apnea as an index.
Experiment 2: hypoxia survival assay
Each group had 10 mice, and each group was administered with 80mg/kg BW by intraperitoneal injection of sodium nitrite (injection amount of 0.1ml/10g BW) 1 hour after the last gavage, immediately counted, and the survival time of the animals was recorded.
Experiment 3: influence of whey protein powder on swimming endurance of mice
And (3) carrying out a swimming experiment with a load of 5% in a swimming box with the water depth of 30cm and the water temperature of 25 +/-0.5 ℃ 0.5h after the last gastric lavage of the mouse, and recording the swimming failure time by taking the judgment end point that the head of the mouse is totally immersed into water and can not float out of the water surface for 8 s.
Experiment 4: determination of the Nitrogen content
After the mice are subjected to last gastric lavage for 0.5h, the mice swim in a swimming box with the water depth of 30cm and the water temperature of 25 +/-0.5 ℃ for 90min, then blood is taken, and the nitrogen content is measured by using a urea nitrogen kit.
Experiment 5: liver glycogen content assay
After the mice are subjected to last intragastric administration for 0.5h, the mice are immediately killed after swimming for 90min in a swimming box with the water depth of 30cm and the water temperature of 25 +/-0.5 ℃, the liver is taken out, and the content of hepatic glycogen is measured.
Test results
Experiment 1 results of atmospheric hypoxia tolerance experiment
Table 1: influence of whey protein powder on mouse hypoxia tolerance time under normal pressure (x + -s, n = 10)
| Group of | Survival time (min) |
| Blank control group | 22.15±5.92 |
| Example 1 | 51.15±5.57*** |
| Example 2 | 50.42±5.44*** |
| Example 3 | 50.67±5.52*** |
| Positive control group | 47.18±5.21*** |
As can be seen from Table 1, the mice of examples 1-3 had significantly higher time to hypoxia than the blank control (P < 0.01). The whey protein powder can improve the normal-pressure hypoxia-resistant capability of mice and enhance the physique of the mice.
Experiment 2 hypoxia survival experimental results
Table 2: influence of whey protein powder on the toxic survival time of mouse sodium nitrite (x + -s, n = 10)
| Group of | Survival time (min) |
| Blank control group | 12.15±2.67 |
| Example 1 | 19.23±3.71** |
| Example 2 | 19.05±3.58** |
| Example 3 | 19.12±3.62** |
| Positive control group | 18.77±3.22** |
As can be seen from Table 2, the survival time of the mice subjected to intragastric administration by the whey protein powder in the examples 1-3 is obviously longer than that of a blank control group (P is less than 0.01), and the whey protein powder can obviously improve the sodium nitrite toxicity resistance of the mice and enhance the physique of the mice.
Experiment 3 influence of whey protein powder on swimming endurance of mice
Table 3: influence of whey protein powder on weight swimming of mice (x + -s, n = 10)
| Group of | Swimming time (min) |
| Blank control group | 327.24±55.16 |
| Example 1 | 726.72±69.16** |
| Example 2 | 712.55±75.12** |
| Example 3 | 717.26±66.52** |
| Positive control group | 652.15±72.59** |
The improvement of the exercise endurance is the most direct expression of the enhancement of the anti-fatigue capability, and the swimming time can reflect the degree of the exercise fatigue of the animals. As can be seen from Table 3, the swimming time of the mice was significantly prolonged in the whey protein powder groups of examples 1-3 as compared to the blank control group. The whey protein powder can obviously prolong the swimming time of mice, namely, enhance the fatigue resistance of the mice.
Experiment 4 measurement results of Nitrogen content
Table 4: influence of whey protein powder on serum urea nitrogen content after swimming of mice (x + -s, n = 10)
| Group of | Serum urea nitrogen content (mmol. L) -1 ) |
| Blank control group | 9.18±1.25 |
| Example 1 | 6.82±0.92* |
| Example 2 | 6.92±0.97* |
| Example 3 | 6.88±0.95* |
| Positive control group | 7.55±1.02* |
As can be seen from Table 4, the mice were subjected to prolonged swimming, and the serum urea nitrogen content of the mice was reduced (P < 0.05) in the groups of examples 1 to 3 as compared with the control group. The whey protein powder disclosed by the invention can reduce the serum urea nitrogen content of a mouse after strenuous exercise, reduce the physiological load of the mouse after exercise, and has a remarkable antagonistic effect on the fatigue of the mouse.
Experiment 5 measurement of hepatic glycogen content
TABLE 5 Effect of whey protein powder on liver glycogen content after swimming of mice (x. + -.s, n = 10)
| Group of | Hepatic glycogen content (mg/g) |
| Blank control group | 9.78±1.26 |
| Example 1 | 16.26±1.44** |
| Example 2 | 15.97±1.49** |
| Example 3 | 16.05±1.51** |
| Positive control group | 15.23±1.42** |
As can be seen from Table 5, liver glycogen of the mice of the example groups of examples 1-3 is obviously improved (P is less than 0.01) compared with the control group after swimming, which indicates that the whey protein powder of the invention can increase liver glycogen storage of the mice and enhance the anti-fatigue constitution of the mice.
According to the test data, the whey protein powder for recovering the functions after exercise has the functions of effectively increasing muscle and weight, reducing fatigue feeling, improving exercise endurance, quickly recovering the functions after exercise and having a good effect on enhancing immunity.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (7)
1. Whey protein powder for recovering functions after exercise is characterized by comprising the following components: casein Glycomacropeptide (CGMP) and whey protein powder (SP-9225).
2. The whey protein powder for recovering after exercise according to claim 1, wherein the mass ratio of the casein glycomacropeptide to the whey protein powder is 1: (3-6).
3. The whey protein powder for post-exercise functional recovery of claim 1, wherein the whey protein powder (SP-9225) has a protein content of 92.25% or more.
4. The whey protein powder for recovery of function after exercise according to claim 1, wherein the preparation method comprises the following steps:
A. collecting fresh whey, filtering with mesh screen and gauze, separating impurities, and collecting supernatant;
B. adding 1.2-1.5mol/L calcium chloride 5-10ml/L into the supernatant, adjusting pH to 4.5-7.5, stirring in 55-65 deg.C water bath for 15-20min, clarifying cheese whey by flocculation centrifugation, microfiltration or flocculation microfiltration to obtain whey protein solution;
C. concentrating the clarified whey protein liquid by using a regenerated cellulose ultrafiltration membrane with the molecular weight cutoff of 15-20KDa under the operating conditions of 25-30 ℃, 0.1-0.2MPa of pressure and 25-40m/s of flow rate, wherein the concentration multiple is 5-10 times to obtain a first whey protein concentrated solution;
D. adding deionized water into the whey protein concentrated solution, wherein the material-liquid ratio is 6-10, the flow rate is 25-40m/s, the pressure is 0.1-0.2MPa, and the molecular weight cut-off of a regenerated cellulose ultrafiltration membrane is 5-10KDa for secondary concentration to remove lactose, so as to obtain a purified whey protein concentrated solution;
E. freeze drying or spray drying the purified whey protein concentrate by a pressure type spray tower;
F. dissolving casein glycomacropeptide in water, and performing ultrasonic dispersion to obtain a casein glycomacropeptide solution;
G. under the action of hot air from bottom to top, the whey protein powder is in a flowing state, and casein glycomacropeptide solution is sprayed in a spraying mode at the same time, and the molding and drying are carried out simultaneously.
5. The whey protein powder for recovery of function after exercise of claim 4, wherein in step E, the spray drying conditions are as follows: the temperature of a sample inlet is 115-135 ℃, the adjustment range of the amount of dry air is 0.50-0.80 cubic meter/min, the pressure of spray is 16 multiplied by 10kPa, the flow of a liquid delivery pump is 1.5-2.5mL/min, the rotating speed of a stirrer is 350-450rpm, and the whey protein powder can be obtained by sieving through a 100-200 mesh sieve.
6. The whey protein powder for post-exercise functional recovery of claim 4, wherein in step F, the mass concentration of the casein glycomacropeptide solution is 1.5-2.5%.
7. The whey protein powder for recovery of function after exercise of claim 4, wherein in step G, the spraying speed is 15-25kg/h.
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