CN115501133A - Sour soybean seed polysaccharide, preparation method thereof and application thereof in preparation of products with skin care effect - Google Patents
Sour soybean seed polysaccharide, preparation method thereof and application thereof in preparation of products with skin care effect Download PDFInfo
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- 150000004676 glycans Chemical class 0.000 title claims abstract description 72
- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 72
- 239000005017 polysaccharide Substances 0.000 title claims abstract description 72
- 244000068988 Glycine max Species 0.000 title claims abstract description 51
- 235000010469 Glycine max Nutrition 0.000 title claims abstract description 51
- 238000002360 preparation method Methods 0.000 title claims description 47
- 230000000694 effects Effects 0.000 title description 17
- 229920001184 polypeptide Polymers 0.000 claims abstract description 58
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 58
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 58
- 238000002156 mixing Methods 0.000 claims abstract description 55
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 46
- 229920001661 Chitosan Polymers 0.000 claims abstract description 44
- 239000003607 modifier Substances 0.000 claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 35
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000004006 olive oil Substances 0.000 claims abstract description 27
- 235000008390 olive oil Nutrition 0.000 claims abstract description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 239000000463 material Substances 0.000 claims abstract description 22
- 235000004298 Tamarindus indica Nutrition 0.000 claims abstract description 21
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims abstract description 11
- 235000017491 Bambusa tulda Nutrition 0.000 claims abstract description 11
- 241001523681 Dendrobium Species 0.000 claims abstract description 11
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims abstract description 11
- 239000011425 bamboo Substances 0.000 claims abstract description 11
- 239000008367 deionised water Substances 0.000 claims abstract description 9
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 9
- 244000082204 Phyllostachys viridis Species 0.000 claims abstract 3
- 238000003756 stirring Methods 0.000 claims description 87
- 239000000047 product Substances 0.000 claims description 36
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 23
- 241000596504 Tamarindus Species 0.000 claims description 19
- 239000002253 acid Substances 0.000 claims description 19
- 238000001914 filtration Methods 0.000 claims description 14
- 230000008014 freezing Effects 0.000 claims description 14
- 238000007710 freezing Methods 0.000 claims description 14
- 239000001509 sodium citrate Substances 0.000 claims description 14
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 14
- 229930003935 flavonoid Natural products 0.000 claims description 9
- 150000002215 flavonoids Chemical class 0.000 claims description 9
- 235000017173 flavonoids Nutrition 0.000 claims description 9
- 108010059892 Cellulase Proteins 0.000 claims description 7
- 108090000790 Enzymes Proteins 0.000 claims description 7
- 102000004190 Enzymes Human genes 0.000 claims description 7
- 229940106157 cellulase Drugs 0.000 claims description 7
- 229940088598 enzyme Drugs 0.000 claims description 7
- 239000000706 filtrate Substances 0.000 claims description 7
- 230000000415 inactivating effect Effects 0.000 claims description 7
- 239000012528 membrane Substances 0.000 claims description 7
- 238000001728 nano-filtration Methods 0.000 claims description 7
- 238000010298 pulverizing process Methods 0.000 claims description 7
- 238000007873 sieving Methods 0.000 claims description 7
- 238000009210 therapy by ultrasound Methods 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- 240000007817 Olea europaea Species 0.000 claims description 6
- 235000013399 edible fruits Nutrition 0.000 claims description 6
- 239000002893 slag Substances 0.000 claims description 6
- 244000048738 European olive Species 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- 235000013361 beverage Nutrition 0.000 claims description 2
- 230000002195 synergetic effect Effects 0.000 abstract description 10
- 230000002087 whitening effect Effects 0.000 abstract description 8
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 abstract description 2
- 229930003944 flavone Natural products 0.000 abstract description 2
- 150000002212 flavone derivatives Chemical class 0.000 abstract description 2
- 235000011949 flavones Nutrition 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 abstract description 2
- 240000004584 Tamarindus indica Species 0.000 abstract 2
- 230000000052 comparative effect Effects 0.000 description 9
- 241001330002 Bambuseae Species 0.000 description 8
- 239000002453 shampoo Substances 0.000 description 5
- WQZGKKKJIJFFOK-SVZMEOIVSA-N (+)-Galactose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-SVZMEOIVSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 description 1
- SRBFZHDQGSBBOR-HWQSCIPKSA-N L-arabinopyranose Chemical compound O[C@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-HWQSCIPKSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 238000005360 mashing Methods 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000036564 melanin content Effects 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000007670 refining Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/365—Hydroxycarboxylic acids; Ketocarboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/645—Proteins of vegetable origin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/736—Chitin; Chitosan; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
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- Health & Medical Sciences (AREA)
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- Epidemiology (AREA)
- Dermatology (AREA)
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- Biotechnology (AREA)
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- Mycology (AREA)
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Abstract
The invention relates to the technical field of sour soybean seed polysaccharide, and particularly discloses sour soybean seed polysaccharide which is prepared by mixing sour soybean seed polysaccharide, a polypeptide modifier and a blending liquid according to a weight ratio of (1-5) to (2) to (5-9). According to the tamarind seed polysaccharide skin care material, tamarind seed polysaccharide, a polypeptide modifier and a blending liquid are proportioned, a dendrobe polypeptide material in the polypeptide modifier is matched with a chitosan agent, then the blending liquid is added, and bamboo leaf flavone, citric acid and deionized water in the blending liquid are matched with the polypeptide modifier, so that the two materials have synergistic effects, the skin care efficiency of a product is enhanced, and the whitening effect of the product is improved; the chitosan agent is matched with chitosan and olive oil, so that the synergistic effect on the dendrobium polypeptide material can be achieved, the mutual combination and coordination among the polypeptide modifier, the blending liquid and the sour soybean seed polysaccharide raw material are enhanced, the synergistic effect of the product is enhanced, and the skin care efficiency is enhanced.
Description
Technical Field
The invention relates to the technical field of sour soybean seed polysaccharide, in particular to sour soybean seed polysaccharide, a preparation method thereof and application thereof in preparing products with skin care effect.
Background
Sour beans, also known as tamarind, tamarind and the like, are produced in tropical Africa and India, and the pulp of the sour bean is rich in polysaccharide of about 72%, protein of 17% and fat of 8%. In the second wartime, sour soybean seed kernels are ground into powder to prepare cakes so as to solve the problem of food shortage. TAM45-NP is a polysaccharide gel acid soybean seed polysaccharide obtained by extracting acid soybean seed polysaccharide from acid soybean seed kernel, refining and purifying, and reducing molecular weight properly, which is mainly neutral polysaccharide composed of D-galactose, D-xylose, and D-glucose (1: 3: 4), and contains small amount of free sugar (glucose and L-arabinose) besides polysaccharide.
The existing sour soybean seed polysaccharide is rarely applied to skin care together with other compositions, has poor matching effect, is used in skin care, is difficult to improve the whitening efficiency and reduce the skin care effect, and therefore, the invention provides the sour soybean seed polysaccharide, the preparation method thereof and the application thereof in preparing products with the skin care effect.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide an acid soybean seed polysaccharide, a preparation method thereof and application thereof in preparing products with skin care effect so as to solve the problems in the background technology.
The technical scheme adopted by the invention for solving the technical problems is as follows:
the invention provides an acid soybean seed polysaccharide, which is prepared by mixing an acid soybean seed polysaccharide, a polypeptide modifier and a blending liquid according to a weight ratio of (1-5) to (2) (5-9);
the preparation method of the polypeptide modifier comprises the following steps:
s01: washing herba Dendrobii with water, draining, pulverizing in a pulverizer, sieving with 30-40 mesh sieve, freezing at-5 deg.C for 1-2 hr, and freezing;
s02: s01, placing the standby dendrobium into water with the temperature of 25-30 ℃, completely unfreezing, then carrying out super-treatment, and finishing the treatment for standby application;
s03: then adding cellulase accounting for 5-10% of the total amount of the S02 product, carrying out enzymolysis treatment, inactivating enzyme after the treatment is finished, and finally filtering by adopting a nanofiltration membrane to obtain a dendrobe polypeptide material;
s04: stirring and mixing the dendrobe polypeptide material and the chitosan agent according to the weight ratio of 6.
Preferably, the temperature of the enzymolysis treatment is 35-39 ℃, and the enzymolysis time is 30-40min.
Preferably, the ultrasonic treatment time is 10-20min, and the ultrasonic power is 350-450W.
Preferably, the preparation method of the chitosan agent comprises the following steps:
adding 5-10 parts of chitosan into 10-20 parts of sodium citrate solution, then adding 1-3 parts of olive oil, stirring at 32-36 ℃ for 20-30min, wherein the stirring speed is 350-450r/min, and obtaining the chitosan agent after the stirring is finished.
Preferably, the mass fraction of the sodium citrate solution is 10-20%.
Preferably, the preparation method of the olive oil agent comprises the following steps:
adding olive fruit into a pounding machine for pounding into slag, adding 3-6 times of ethanol, stirring at 40-45 deg.C for 10-20min at 500-700r/min under 10-20MPa, removing alcohol, and filtering to obtain filtrate, i.e. olive oil solution.
The inventor of the invention finds that the whitening effect of the product is poor without adding a polypeptide modifier, and meanwhile, the performance of the product is poor without adding a chitosan agent and an olive oil agent, and the whitening effect of the product can be enhanced by using the chitosan agent;
the polypeptide modifier and the blending liquid can achieve a synergistic effect, and the performance effect of the product is enhanced together.
The inventor of the invention finds that the polypeptide modifier has the most remarkable effect performance in improving products, the product performance is poor when the chitosan agent is not added and the olive oil agent is not added in the chitosan agent, the synergistic effect with a blending liquid can be strongest only when the polypeptide modifier is prepared by the method of the invention, and the improvement efficiency is highest.
Preferably, ultrasonic auxiliary treatment is also adopted in the stirring, and the ultrasonic power is 300-350W.
Preferably, the preparation method of the preparation liquid comprises the following steps: the bamboo leaf flavonoid, the citric acid and the deionized water are prepared according to the weight ratio of 1 (3-5) to 8.
The invention also provides a preparation method of the sour soybean seed polysaccharide, which comprises the following steps: sequentially adding the sour soybean seed polysaccharide, the polypeptide modifier and the blending liquid into a stirrer, stirring and mixing fully, wherein the stirring speed is 500-1000r/min, the stirring time is 30-40min, the stirring temperature is 35-40 ℃, and stirring is finished to obtain the sour soybean seed polysaccharide.
The invention also provides application of the preparation method of the sour soybean seed polysaccharide in products with skin care effects.
Compared with the prior art, the invention has the following beneficial effects:
according to the tamarind seed polysaccharide skin care material, tamarind seed polysaccharide, a polypeptide modifier and a blending liquid are proportioned, a dendrobe polypeptide material in the polypeptide modifier is matched with a chitosan agent, then the blending liquid is added, and bamboo leaf flavone, citric acid and deionized water in the blending liquid are matched with the polypeptide modifier, so that the two materials have synergistic effects, the skin care efficiency of a product is enhanced, and the whitening effect of the product is improved; the chitosan agent is matched with chitosan and olive oil, so that the synergistic effect on the dendrobium polypeptide material can be achieved, the mutual combination and coordination among the polypeptide modifier, the blending liquid and the sour soybean seed polysaccharide raw material are enhanced, the synergistic effect of the product is enhanced, and the skin care efficiency is enhanced.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to specific embodiments, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The acid soybean seed polysaccharide is prepared by mixing acid soybean seed polysaccharide, a polypeptide modifier and a blending liquid according to a weight ratio of (1-5) to (2) (5-9);
the preparation method of the polypeptide modifier comprises the following steps:
s01: washing herba Dendrobii with water, draining, pulverizing with a pulverizer, sieving with 30-40 mesh sieve, freezing at-5 deg.C for 1-2 hr, and freezing;
s02: s01, placing the standby dendrobium into water with the temperature of 25-30 ℃, completely unfreezing, then carrying out super-treatment, and finishing the treatment for standby application;
s03: then adding cellulase accounting for 5-10% of the total amount of the S02 product, carrying out enzymolysis treatment, inactivating enzyme after the treatment is finished, and finally filtering by adopting a nanofiltration membrane to obtain a dendrobe polypeptide material;
s04: stirring and mixing the dendrobe polypeptide material and the chitosan agent according to the weight ratio of 6.
The temperature of the enzymolysis treatment in the embodiment is 35-39 ℃, and the enzymolysis time is 30-40min.
The ultrasonic treatment time of the embodiment is 10-20min, and the ultrasonic power is 350-450W.
The preparation method of the chitosan agent of the embodiment comprises the following steps:
adding 5-10 parts of chitosan into 10-20 parts of sodium citrate solution, then adding 1-3 parts of olive oil, stirring at 32-36 ℃ for 20-30min at the stirring speed of 350-450r/min, and obtaining the chitosan agent after stirring.
The mass fraction of the sodium citrate solution in this example is 10-20%.
The preparation method of the olive oil agent in the embodiment comprises the following steps:
adding olive fruit into a pounding machine, pounding into slag, adding 3-6 times of ethanol, stirring at 40-45 deg.C for 10-20min at a stirring speed of 500-700r/min under a stirring pressure of 10-20MPa, removing ethanol, and filtering to obtain filtrate, i.e. olive oil solution.
In the stirring of the embodiment, ultrasonic auxiliary treatment is also adopted, and the ultrasonic power is 300-350W.
The preparation method of the blending liquid in this embodiment is as follows: the bamboo leaf flavonoid beverage is prepared from bamboo leaf flavonoid, citric acid and deionized water according to the weight ratio of 1 (3-5) to 8.
The preparation method of the acid soybean seed polysaccharide comprises the following steps: sequentially adding the sour soybean seed polysaccharide, the polypeptide modifier and the blending liquid into a stirrer, stirring and mixing fully, wherein the stirring speed is 500-1000r/min, the stirring time is 30-40min, the stirring temperature is 35-40 ℃, and stirring is finished to obtain the sour soybean seed polysaccharide.
The preparation method of the tamarind seed polysaccharide is applied to shampoo products with skin care effects.
Example 1.
The sour soybean seed polysaccharide is prepared by mixing sour soybean seed polysaccharide, a polypeptide modifier and a blending liquid according to a weight ratio of 1;
the preparation method of the polypeptide modifier comprises the following steps:
s01: washing herba Dendrobii with water, draining, pulverizing with a pulverizer, sieving with 30 mesh sieve, freezing at-5 deg.C for 1 hr, and freezing;
s02: s01, placing the standby dendrobium in water at 25 ℃, completely unfreezing, then performing super-treatment, and finishing the treatment for standby application;
s03: then adding cellulase accounting for 5 percent of the total amount of the S02 product, carrying out enzymolysis treatment, inactivating enzyme after the treatment is finished, and finally filtering by adopting a nanofiltration membrane to obtain the dendrobium polypeptide material;
s04: stirring and mixing the dendrobe polypeptide material and the chitosan agent according to the weight ratio of 6.
In this embodiment, the temperature of the enzymolysis treatment is 35 ℃, and the enzymolysis time is 30min.
The ultrasonic treatment time of this example was 10min, and the ultrasonic power was 350W.
The preparation method of the chitosan agent of the embodiment comprises the following steps:
adding 5 parts of chitosan into 10 parts of sodium citrate solution, then adding 1 part of olive oil solution, stirring at 32 ℃ for 20min at the stirring speed of 350r/min, and obtaining the chitosan agent after the stirring is finished.
The sodium citrate solution of this example was 10% by mass.
The preparation method of the olive oil agent in the embodiment comprises the following steps:
adding olive fruits into a pounding machine for pounding into slag, then adding 3 times of ethanol, stirring for 10min at 40 ℃, the stirring speed is 500r/min, the stirring pressure is 10MPa, after stirring, removing alcohol, and filtering to obtain filtrate, namely olive oil.
In the stirring of this embodiment, ultrasonic auxiliary treatment is also adopted, and the ultrasonic power is 300W.
The preparation method of the blending liquid in this embodiment is as follows: the bamboo leaf flavonoid, the citric acid and the deionized water are prepared according to the weight ratio of 1.
The preparation method of the tamarind seed polysaccharide comprises the following steps: sequentially adding the sour soybean seed polysaccharide, the polypeptide modifier and the blending liquid into a stirrer, stirring and mixing fully, wherein the stirring speed is 500r/min, the stirring time is 30min, the stirring temperature is 35 ℃, and stirring is finished to obtain the sour soybean seed polysaccharide.
The preparation method of the tamarind seed polysaccharide is applied to shampoo products with skin care effects.
Example 2.
The acid soybean seed polysaccharide is prepared by mixing the acid soybean seed polysaccharide, a polypeptide modifier and a blending liquid according to a weight ratio of 5;
the preparation method of the polypeptide modifier comprises the following steps:
s01: washing herba Dendrobii with water, draining, pulverizing in a pulverizer, sieving with 40 mesh sieve, freezing at-5 deg.C for 2 hr, and freezing;
s02: s01, placing the standby dendrobium into water with the temperature of 30 ℃, completely unfreezing, then carrying out super-treatment, and finishing the treatment for standby application;
s03: then adding cellulase accounting for 10 percent of the total amount of the S02 product, carrying out enzymolysis treatment, inactivating enzyme, and finally filtering by adopting a nanofiltration membrane to obtain a dendrobe polypeptide material;
s04: stirring and mixing the dendrobe polypeptide material and the chitosan agent according to the weight ratio of 6.
The temperature of the enzymolysis treatment in this embodiment is 39 ℃, and the enzymolysis time is 40min.
The ultrasonic treatment time of the present embodiment was 20min, and the ultrasonic power was 450W.
The preparation method of the chitosan agent of the embodiment comprises the following steps:
adding 10 parts of chitosan into 20 parts of sodium citrate solution, then adding 3 parts of olive oil, stirring at 36 ℃ for 30min at the stirring speed of 450r/min, and obtaining the chitosan agent after the stirring is finished.
The mass fraction of the sodium citrate solution in this example was 20%.
The preparation method of the olive oil agent in the embodiment comprises the following steps:
adding olive fruits into a pounding machine to be pounded into slag, then adding 6 times of ethanol, stirring for 20min at 45 ℃, wherein the stirring speed is 700r/min, the stirring pressure is 20MPa, and after the stirring, removing the alcohol and filtering to obtain filtrate, namely the olive oil agent.
In the stirring of the present embodiment, ultrasonic auxiliary treatment is also adopted, and the ultrasonic power is 350W.
The preparation method of the blending liquid in this embodiment is as follows: the bamboo leaf flavonoid, the citric acid and the deionized water are prepared according to the weight ratio of 1.
The preparation method of the acid soybean seed polysaccharide comprises the following steps: sequentially adding the sour soybean seed polysaccharide, the polypeptide modifier and the blending liquid into a stirrer, stirring and fully mixing at the stirring speed of 1000r/min for 40min at the stirring temperature of 40 ℃, and finishing stirring to obtain the sour soybean seed polysaccharide.
The preparation method of the tamarind seed polysaccharide is applied to shampoo products with skin care effects.
Example 3.
The sour soybean seed polysaccharide is prepared by mixing sour soybean seed polysaccharide, a polypeptide modifier and a blending liquid according to a weight ratio of 3;
the preparation method of the polypeptide modifier comprises the following steps:
s01: washing herba Dendrobii with water, draining, pulverizing with a pulverizer, sieving with 35 mesh sieve, freezing at-5 deg.C for 1.5 hr, and freezing;
s02: placing the dendrobium used in S01 in water with the temperature of 27.5 ℃, completely unfreezing, then carrying out super-treatment, and finishing the treatment for standby application;
s03: then adding cellulase accounting for 7.5 percent of the total amount of the S02 product, carrying out enzymolysis treatment, inactivating enzyme after the treatment is finished, and finally filtering by adopting a nanofiltration membrane to obtain the dendrobium polypeptide material;
s04: stirring and mixing the dendrobe polypeptide material and the chitosan agent according to the weight ratio of 6.
In this embodiment, the temperature of the enzymolysis treatment is 37 ℃, and the enzymolysis time is 35min.
The ultrasonic treatment time of this example was 15min, and the ultrasonic power was 400W.
The preparation method of the chitosan agent of the embodiment comprises the following steps:
adding 7.5 parts of chitosan into 15 parts of sodium citrate solution, then adding 2 parts of olive oil solution, stirring for 5min at 34 ℃, wherein the stirring speed is 400r/min, and obtaining the chitosan agent after the stirring is finished.
The mass fraction of the sodium citrate solution in this example is 10-20%.
The preparation method of the olive oil agent in the embodiment comprises the following steps:
adding fructus Oleae Europaeae into a pounder, mashing to obtain residue, adding 4.5 times of ethanol, stirring at 42.5 deg.C for 15min at a rotation speed of 600r/min under a stirring pressure of 105MPa, removing ethanol, and filtering to obtain filtrate, i.e. oleum Olivarum oil.
In the stirring of this example, ultrasonic auxiliary treatment was also used, and the ultrasonic power was 325W.
The preparation method of the blending liquid in this embodiment is as follows: the bamboo leaf flavonoid, the citric acid and the deionized water are prepared according to the weight ratio of 1.
The preparation method of the tamarind seed polysaccharide comprises the following steps: sequentially adding the sour soybean seed polysaccharide, the polypeptide modifier and the blending liquid into a stirrer, stirring and mixing fully, wherein the stirring speed is 750r/min, the stirring time is 35min, the stirring temperature is 37.5 ℃, and stirring is finished to obtain the sour soybean seed polysaccharide.
The preparation method of the tamarind seed polysaccharide is applied to shampoo products with skin care effects.
Example 4.
The acid soybean seed polysaccharide is prepared by mixing the acid soybean seed polysaccharide, a polypeptide modifier and a blending liquid according to the weight ratio of (2);
the preparation method of the polypeptide modifier comprises the following steps:
s01: washing herba Dendrobii with water, draining, pulverizing in a pulverizer, sieving with 32 mesh sieve, freezing at-5 deg.C for 1.2 hr, and freezing;
s02: s01, placing the standby dendrobium into water with the temperature of 27 ℃, completely unfreezing, then carrying out super-treatment, and finishing the treatment for standby application;
s03: then adding cellulase accounting for 6 percent of the total amount of S02 products, carrying out enzymolysis treatment, inactivating enzyme, and finally filtering by adopting a nanofiltration membrane to obtain a dendrobe polypeptide material;
s04: stirring and mixing the dendrobe polypeptide material and the chitosan agent according to the weight ratio of 6.
The temperature of the enzymolysis treatment in this embodiment is 36 ℃, and the enzymolysis time is 32min.
The ultrasonic treatment time of this embodiment is 12min, and the ultrasonic power is 360W.
The preparation method of the chitosan agent of the embodiment comprises the following steps:
adding 6 parts of chitosan into 12 parts of sodium citrate solution, then adding 2 parts of olive oil, stirring at 33 ℃ for 22min at the stirring speed of 360r/min, and finishing stirring to obtain the chitosan agent.
The mass fraction of the sodium citrate solution in this example was 12%.
The preparation method of the olive oil agent in the embodiment comprises the following steps:
adding olive fruits into a pounding machine to be pounded into slag, then adding 4 times of ethanol, stirring at 42 ℃ for 12min at the rotation speed of 550r/min and the stirring pressure of 12MPa, removing alcohol after stirring, and filtering to obtain filtrate, namely olive oil.
In the stirring of the present embodiment, ultrasonic auxiliary treatment is also adopted, and the ultrasonic power is 310W.
The preparation method of the blending liquid in this embodiment is as follows: the bamboo leaf flavonoid, the citric acid and the deionized water are prepared according to the weight ratio of 1.
The preparation method of the acid soybean seed polysaccharide comprises the following steps: sequentially adding the sour soybean seed polysaccharide, the polypeptide modifier and the blending liquid into a stirrer, stirring and mixing fully, wherein the stirring speed is 600r/min, the stirring time is 32min, the stirring temperature is 36 ℃, and stirring is finished to obtain the sour soybean seed polysaccharide.
The preparation method of the tamarind seed polysaccharide is applied to shampoo products with skin care effects.
Comparative example 1.
Unlike example 3, no polypeptide modifier was added.
Comparative example 2.
The difference from the example 3 is that no chitosan agent is added in the polypeptide modifier.
Comparative example 3.
The difference from example 3 is that no olive oil was added to the chitosan agent.
Comparative example 4.
The difference from example 3 is that no formulation was added.
The products of examples 1-4 and comparative examples 1-4 were tested for their performance as follows:
20 female volunteers with dark facial complexion are randomly selected from each group, the age of the volunteers is 30-50 years, hormone medicines are not used in nearly one month, 2mL of skin care water added with the skin care water obtained in the embodiment and the comparative example is taken by each group of female volunteers every day, the skin care water is uniformly smeared on the face, the female volunteers are insisted on using for 45 days, and the MI value of the skin melanin content of each group of volunteers is detected by using the skin melanin of MX 18. Wherein a higher MI value measurement indicates a higher pigment content.
As seen from comparative examples 1 to 5 and examples 1 to 4;
the product of example 3 has the strongest whitening effect; through the comparative examples 1-3, the whitening effect of the product is poor without adding the polypeptide modifier, and meanwhile, the performance of the product is poor without adding the chitosan agent and the olive oil agent, and the whitening effect of the product can be enhanced by using the chitosan agent;
meanwhile, the comparative example 4 shows that the performance of the product is deteriorated without adding the blending liquid, and the polypeptide modifier and the blending liquid of the invention can achieve a synergistic effect and enhance the performance effect of the product.
The polypeptide modifier has the advantages that the effect performance of the product is improved most obviously, the chitosan agent is not added, and the olive oil agent is not added into the chitosan agent, so that the product performance is poor, only the polypeptide modifier prepared by the method can have the strongest synergistic effect with the blending liquid, and the improvement efficiency is highest.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. The acid soybean seed polysaccharide is characterized in that the acid soybean seed polysaccharide is prepared by mixing acid soybean seed polysaccharide, a polypeptide modifier and a blending liquid according to a weight ratio of (1-5) to (2) to (5-9);
the preparation method of the polypeptide modifier comprises the following steps:
s01: washing herba Dendrobii with water, draining, pulverizing in a pulverizer, sieving with 30-40 mesh sieve, freezing at-5 deg.C for 1-2 hr, and freezing;
s02: s01, placing the standby dendrobium into water with the temperature of 25-30 ℃, completely unfreezing, then carrying out super-treatment, and finishing the treatment for standby application;
s03: then adding cellulase accounting for 5-10% of the total amount of S02 products, carrying out enzymolysis treatment, inactivating enzyme, and finally filtering by adopting a nanofiltration membrane to obtain a dendrobe polypeptide material;
s04: stirring and mixing the dendrobe polypeptide material and the chitosan agent according to the weight ratio of 6.
2. The tamarind seed polysaccharide according to claim 1, wherein the enzymolysis temperature is 35-39 ℃, and the enzymolysis time is 30-40min.
3. The tamarind seed polysaccharide according to claim 1, wherein the ultrasonic treatment time is 10-20min, and the ultrasonic power is 350-450W.
4. The tamarind seed polysaccharide according to claim 1, wherein the chitosan agent is prepared by the following steps:
adding 5-10 parts of chitosan into 10-20 parts of sodium citrate solution, then adding 1-3 parts of olive oil, stirring at 32-36 ℃ for 20-30min, wherein the stirring speed is 350-450r/min, and obtaining the chitosan agent after the stirring is finished.
5. The tamarind seed polysaccharide according to claim 4, wherein the mass fraction of the sodium citrate solution is 10-20%.
6. The tamarind seed polysaccharide of claim 4, wherein said olive oil is prepared by the following steps:
adding olive fruit into a pounding machine for pounding into slag, adding 3-6 times of ethanol, stirring at 40-45 deg.C for 10-20min at 500-700r/min under 10-20MPa, removing alcohol, and filtering to obtain filtrate, i.e. olive oil solution.
7. The tamarind seed polysaccharide of claim 6, wherein ultrasonic-assisted treatment is further employed in the stirring, and the ultrasonic power is 300-350W.
8. The tamarind seed polysaccharide as claimed in claim 1, wherein the preparation method of the preparation liquid comprises: the bamboo leaf flavonoid beverage is prepared from bamboo leaf flavonoid, citric acid and deionized water according to the weight ratio of 1 (3-5) to 8.
9. A method for preparing the tamarind seed polysaccharide according to any one of claims 1 to 8, comprising the steps of: sequentially adding the sour soybean seed polysaccharide, the polypeptide modifier and the blending liquid into a stirrer, stirring and mixing fully, wherein the stirring speed is 500-1000r/min, the stirring time is 30-40min, the stirring temperature is 35-40 ℃, and stirring is finished to obtain the sour soybean seed polysaccharide.
10. Use of the acid soybean seed polysaccharide of claim 9 in a skin care product.
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| CN116549316A (en) * | 2023-04-24 | 2023-08-08 | 广州领衔生物科技有限公司 | Composition containing blue copper peptide freeze-dried powder and application of composition in cosmetics |
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Application publication date: 20221223 |