CN115490339A - Preparation method of bacteria-algae composite preparation suitable for rural domestic sewage treatment - Google Patents
Preparation method of bacteria-algae composite preparation suitable for rural domestic sewage treatment Download PDFInfo
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- CN115490339A CN115490339A CN202211310135.9A CN202211310135A CN115490339A CN 115490339 A CN115490339 A CN 115490339A CN 202211310135 A CN202211310135 A CN 202211310135A CN 115490339 A CN115490339 A CN 115490339A
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- bacteria
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- chlorella pyrenoidosa
- bactericide
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- 238000002360 preparation method Methods 0.000 title claims abstract description 31
- 239000010865 sewage Substances 0.000 title claims abstract description 29
- 239000002131 composite material Substances 0.000 title claims abstract description 11
- 241000894006 Bacteria Species 0.000 claims abstract description 78
- 235000007091 Chlorella pyrenoidosa Nutrition 0.000 claims abstract description 37
- 150000001875 compounds Chemical class 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 238000012258 culturing Methods 0.000 claims abstract description 25
- 230000001580 bacterial effect Effects 0.000 claims abstract description 23
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 238000000855 fermentation Methods 0.000 claims description 40
- 230000004151 fermentation Effects 0.000 claims description 40
- 230000000243 photosynthetic effect Effects 0.000 claims description 34
- 244000249214 Chlorella pyrenoidosa Species 0.000 claims description 32
- 239000001963 growth medium Substances 0.000 claims description 30
- 239000003795 chemical substances by application Substances 0.000 claims description 29
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 24
- 230000001546 nitrifying effect Effects 0.000 claims description 24
- 230000000844 anti-bacterial effect Effects 0.000 claims description 20
- 239000003899 bactericide agent Substances 0.000 claims description 20
- 238000011218 seed culture Methods 0.000 claims description 15
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 10
- 241000108664 Nitrobacteria Species 0.000 claims description 10
- 238000005286 illumination Methods 0.000 claims description 10
- 241000195493 Cryptophyta Species 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 5
- 230000000249 desinfective effect Effects 0.000 claims description 5
- 230000003203 everyday effect Effects 0.000 claims description 5
- 241000235342 Saccharomycetes Species 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 5
- 239000003344 environmental pollutant Substances 0.000 abstract description 2
- 231100000719 pollutant Toxicity 0.000 abstract description 2
- 241000195652 Auxenochlorella pyrenoidosa Species 0.000 abstract 5
- 239000002068 microbial inoculum Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 6
- 239000002994 raw material Substances 0.000 description 5
- 230000009471 action Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 239000008139 complexing agent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a preparation method of a chlorella-pyrenoidosa composite preparation suitable for rural domestic sewage treatment, which is characterized by comprising the steps of culturing chlorella pyrenoidosa, culturing composite bacterial liquid and mixing the chlorella pyrenoidosa with the composite bacterial liquid, wherein the chlorella pyrenoidosa and the composite bacterial liquid comprise the following components in parts by weight: 4-12 parts of chlorella pyrenoidosa and 8-16 parts of compound bacterial liquid. Compared with a single system consisting of traditional microalgae or functional bacteria, the invention has higher stability of a microecological community, can adapt to rural domestic sewage with obvious water quantity and water quality fluctuation, and has better impact load resistance of pollutants.
Description
Technical Field
The invention relates to the technical field of rural domestic sewage treatment, in particular to a preparation method of a bacteria-algae composite preparation suitable for rural domestic sewage treatment.
Background
With the advancement of the joy of villages and the rapid development of rural economy, the popularization rate of water supply in rural areas is gradually improved and is over 80 percent at present. However, the sewage treatment condition is not optimistic, and according to the statistical data of housing and urban and rural construction departments, the number of villages for treating domestic sewage in China is 3156 and the percentage of villages is only 33.3 percent by 2019. At present, the sewage treatment rate of cities reaches 96.81 percent, but the sewage treatment rate of towns and villages built in China is increased very slowly, the sewage treatment of the villages needs to be paid attention, and the effective treatment of the domestic sewage in the villages is very slow.
The current commonly used rural domestic sewage treatment process mainly comprises a stabilization pond, an artificial wetland, a biological ecological filter, anaerobic and aerobic biological treatment, biological contact oxidation and the like. However, the problem of the disordered discharge of the rural sewage in China still stands out due to the reasons that the regional adaptability of rural sewage treatment technologies and modes is insufficient, the local government responsibility is not in place, the long-acting operation management mechanism is deficient, the technology and the standard are to be perfected and the like, and mainly shows that the treatment rate of the rural sewage is low, the discharge capacity is large, and the collection and treatment facilities are imperfect; the pollution of the country river channel and the damage of the surrounding ecological environment are serious.
The algae-bacteria symbiotic system can treat wastewater through the synergistic effect between microalgae and bacteria, is simple to operate, has no secondary pollution, is beneficial to harvesting microalgae biomass, and has important environmental and economic benefits.
Disclosure of Invention
The invention aims to provide a preparation method of a bacteria-algae compound preparation suitable for rural domestic sewage treatment, so as to solve the problems in the background technology.
In order to realize the purpose, the invention provides the following technical scheme: the preparation method of the chlorella pyrenoidosa compound preparation suitable for rural domestic sewage treatment is characterized by comprising the steps of culturing chlorella pyrenoidosa, culturing compound bacterial liquid and mixing the chlorella pyrenoidosa with the compound bacterial liquid, wherein the chlorella pyrenoidosa and the compound bacterial liquid comprise the following components in parts by weight: 4-12 parts of chlorella pyrenoidosa and 8-16 parts of compound bacterial liquid.
Preferably, the culture of the chlorella pyrenoidosa comprises the following steps:
s1, adjusting the pH of a BG11 culture medium to 7.1 +/-0.2 by using 1mol/L hydrochloric acid, then carrying out autoclaving for 30min at 121 ℃, cooling to room temperature, and then disinfecting for 30min by using ultraviolet rays;
s2, under the aseptic condition, inoculating the chlorella pyrenoidosa algae liquid into the BG11 culture medium cultured in the step S1, and performing continuous illumination culture by using a white fluorescent lamp, wherein the illumination intensity is 4000lux, the temperature is 25 +/-2 ℃, and the culture is performed for 24-96h by shaking 3 times every day.
Preferably, the culturing of the compound bacterial liquid comprises the following steps:
inoculating aerobic denitrifying bacteria into an aerobic denitrifying bacteria seed culture medium for culturing to obtain aerobic denitrifying bacteria;
inoculating aerobic denitrifying bacteria into a fermentation tank for amplified fermentation to obtain an aerobic denitrifying bacteria agent;
inoculating nitrifying bacteria into a nitrifying bacteria seed culture medium for culturing to obtain nitrifying bacteria;
inoculating nitrobacteria into a fermentation tank for amplified fermentation to obtain a nitrobacteria agent;
inoculating photosynthetic bacteria into a photosynthetic bacteria seed culture medium for culturing to obtain photosynthetic bacteria;
inoculating photosynthetic bacteria into a fermentation tank for amplified fermentation to obtain a photosynthetic bacteria microbial inoculum;
inoculating yeast into yeast strain culture medium, and culturing to obtain yeast;
inoculating saccharomycetes into a fermentation tank for amplified fermentation to obtain a saccharomycetes microbial inoculum;
mixing 2-4 parts of aerobic denitrifying bacteria agent, 2-4 parts of nitrifying bacteria agent, 3-6 parts of photosynthetic bacteria agent and 1-2 parts of yeast agent.
Compared with the prior art, the invention has the beneficial effects that:
compared with a single system consisting of traditional microalgae or functional bacteria, the invention has higher stability of a microecological community, can adapt to rural domestic sewage with obvious water quantity and water quality fluctuation, and has better impact load resistance of pollutants.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
Example 1:
the preparation method of the chlorella pyrenoidosa compound preparation suitable for rural domestic sewage treatment comprises chlorella pyrenoidosa and compound bacterial liquid, wherein the chlorella pyrenoidosa and the compound bacterial liquid are prepared from the following raw materials in parts by weight: 4 parts of aerobic denitrifying bactericides: 4 parts of nitrifying bacteria agent: 4 parts of photosynthetic bacteria agent: 6 parts of yeast agent: and 2 parts of the raw materials.
The preparation method of the fungus-algae composite preparation suitable for rural domestic sewage treatment comprises the culture of chlorella pyrenoidosa, and comprises the following steps:
s1, adjusting the pH of a BG11 culture medium to 7.1 +/-0.2 by using 1mol/L hydrochloric acid, then carrying out autoclaving at 121 ℃ for 30min, cooling to room temperature, and then disinfecting for 30min by using ultraviolet rays;
s2, inoculating the chlorella pyrenoidosa algae liquid into the BG11 culture medium cultured in the step S1 under the aseptic condition, and performing continuous illumination culture by using a white fluorescent lamp, wherein the illumination intensity is 4000lux, the temperature is 25 +/-2 ℃, and the culture is performed for 24-96h by shaking 3 times every day.
The culture of the compound bacterial liquid comprises the following steps:
inoculating aerobic denitrifying bacteria into an aerobic denitrifying bacteria seed culture medium for culturing to obtain aerobic denitrifying bacteria;
inoculating aerobic denitrifying bacteria into a fermentation tank for amplified fermentation to obtain an aerobic denitrifying bacteria microbial inoculum;
inoculating nitrifying bacteria into a nitrifying bacteria seed culture medium for culturing to obtain nitrifying bacteria;
inoculating nitrobacteria into a fermentation tank for amplified fermentation to obtain a nitrobacteria agent;
inoculating photosynthetic bacteria into a photosynthetic bacteria seed culture medium for culturing to obtain photosynthetic bacteria;
inoculating photosynthetic bacteria into a fermentation tank for amplified fermentation to obtain a photosynthetic bacteria agent;
inoculating yeast into a yeast strain culture medium to culture to obtain yeast;
inoculating the microzyme into a fermentation tank for amplified fermentation to obtain the microzyme microbial inoculum.
Mixing Chlorella pyrenoidosa, aerobic denitrifying bactericide, nitrifying bactericide, photosynthetic bactericide and yeast agent, and stirring to obtain the final product.
Example 2:
the preparation method of the chlorella pyrenoidosa compound preparation suitable for rural domestic sewage treatment comprises chlorella pyrenoidosa and compound bacterial liquid, wherein the chlorella pyrenoidosa and the compound bacterial liquid are prepared from the following raw materials in parts by weight: 6 parts of aerobic denitrifying bactericides: 3 parts of nitrifying bacteria agent: 4 parts of photosynthetic bacteria agent: 5 parts of yeast agent: and 2 parts of the raw materials.
The preparation method of the fungus-algae compound preparation suitable for rural domestic sewage treatment comprises the culture of chlorella pyrenoidosa, and comprises the following steps:
s1, adjusting the pH of a BG11 culture medium to 7.1 +/-0.2 by using 1mol/L hydrochloric acid, then carrying out autoclaving for 30min at 121 ℃, cooling to room temperature, and then disinfecting for 30min by using ultraviolet rays;
s2, under the aseptic condition, inoculating the chlorella pyrenoidosa algae liquid into the BG11 culture medium cultured in the step S1, and performing continuous illumination culture by using a white fluorescent lamp, wherein the illumination intensity is 4000lux, the temperature is 25 +/-2 ℃, and the culture is performed for 24-96h by shaking 3 times every day.
The culture of the compound bacterial liquid comprises the following steps:
inoculating aerobic denitrifying bacteria into an aerobic denitrifying bacteria seed culture medium for culturing to obtain aerobic denitrifying bacteria;
inoculating aerobic denitrifying bacteria into a fermentation tank for amplified fermentation to obtain an aerobic denitrifying bacteria agent;
inoculating nitrifying bacteria into a nitrifying bacteria seed culture medium for culturing to obtain nitrifying bacteria;
inoculating nitrobacteria into a fermentation tank for amplified fermentation to obtain a nitrobacteria agent;
inoculating photosynthetic bacteria into a photosynthetic bacteria seed culture medium for culturing to obtain photosynthetic bacteria;
inoculating photosynthetic bacteria into a fermentation tank for amplified fermentation to obtain a photosynthetic bacteria microbial inoculum;
inoculating yeast into yeast strain culture medium, and culturing to obtain yeast;
inoculating the microzyme into a fermentation tank for amplified fermentation to obtain the microzyme microbial inoculum.
Mixing Chlorella pyrenoidosa, aerobic denitrifying bactericide, nitrifying bactericide, photosynthetic bactericide and yeast agent, and stirring to obtain the final product.
Example 3:
the preparation method of the chlorella pyrenoidosa compound preparation suitable for rural domestic sewage treatment comprises the chlorella pyrenoidosa and compound bacterial liquid, wherein the chlorella pyrenoidosa and the compound bacterial liquid are prepared from the following raw materials in parts by weight: 8 parts of aerobic denitrifying bactericides: 3 parts of nitrifying bacteria agent: 4 parts of photosynthetic bacteria agent: 4 parts of yeast agent: 1 part.
The preparation method of the fungus-algae composite preparation suitable for rural domestic sewage treatment comprises the culture of chlorella pyrenoidosa, and comprises the following steps:
s1, adjusting the pH of a BG11 culture medium to 7.1 +/-0.2 by using 1mol/L hydrochloric acid, then carrying out autoclaving at 121 ℃ for 30min, cooling to room temperature, and then disinfecting for 30min by using ultraviolet rays;
s2, under the aseptic condition, inoculating the chlorella pyrenoidosa algae liquid into the BG11 culture medium cultured in the step S1, and performing continuous illumination culture by using a white fluorescent lamp, wherein the illumination intensity is 4000lux, the temperature is 25 +/-2 ℃, and the culture is performed for 24-96h by shaking 3 times every day.
The culture of the compound bacterial liquid comprises the following steps:
inoculating aerobic denitrifying bacteria into an aerobic denitrifying bacteria seed culture medium for culturing to obtain aerobic denitrifying bacteria;
inoculating aerobic denitrifying bacteria into a fermentation tank for amplified fermentation to obtain an aerobic denitrifying bacteria agent;
inoculating nitrifying bacteria into a nitrifying bacteria seed culture medium for culturing to obtain nitrifying bacteria;
inoculating nitrobacteria into a fermentation tank for amplified fermentation to obtain a nitrobacteria agent;
inoculating photosynthetic bacteria into a photosynthetic bacteria seed culture medium for culturing to obtain photosynthetic bacteria;
inoculating photosynthetic bacteria into a fermentation tank for amplified fermentation to obtain a photosynthetic bacteria microbial inoculum;
inoculating yeast into yeast strain culture medium, and culturing to obtain yeast;
inoculating the microzyme into a fermentation tank for amplified fermentation to obtain the microzyme preparation.
Mixing Chlorella pyrenoidosa, aerobic denitrifying bactericide, nitrifying bactericide, photosynthetic bactericide and yeast agent, and stirring to obtain bacterium-algae compound agent.
And (3) effect testing:
the using amount of the bacteria and algae complexing agent in the sewage is 10g/m 3 Compared with samples without the compound microbial inoculum, the method has obvious effects on COD, NH4+ -N, NO3- -N, TP, chromaticity, turbidity and the removal rate of refractory substances.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. The preparation method of the chlorella pyrenoidosa compound preparation suitable for rural domestic sewage treatment is characterized by comprising the steps of culturing chlorella pyrenoidosa, culturing compound bacterial liquid and mixing the chlorella pyrenoidosa with the compound bacterial liquid, wherein the chlorella pyrenoidosa and the compound bacterial liquid comprise the following components in parts by weight: 4-9 parts of chlorella pyrenoidosa and 8-16 parts of compound bacterial liquid.
2. The preparation method of the bacteria-algae composite preparation suitable for rural domestic sewage treatment according to claim 1, characterized in that: the culture of the chlorella pyrenoidosa comprises the following steps:
s1, adjusting the pH of a BG11 culture medium to 7.1 +/-0.2 by using 1mol/L hydrochloric acid, then carrying out autoclaving for 30min at 121 ℃, cooling to room temperature, and then disinfecting for 30min by using ultraviolet rays;
s2, inoculating the chlorella pyrenoidosa algae liquid into the BG11 culture medium cultured in the step S1 under the aseptic condition, and performing continuous illumination culture by using a white fluorescent lamp, wherein the illumination intensity is 4000lux, the temperature is 25 +/-2 ℃, and the culture is performed for 24-96h by shaking 3 times every day.
3. The preparation method of the bacteria-algae composite preparation suitable for rural domestic sewage treatment according to claim 1, characterized in that: the culture of the compound bacterial liquid comprises the following steps:
inoculating aerobic denitrifying bacteria into an aerobic denitrifying bacteria seed culture medium for culturing to obtain aerobic denitrifying bacteria;
inoculating aerobic denitrifying bacteria into a fermentation tank for amplified fermentation to obtain an aerobic denitrifying bacteria agent;
inoculating nitrifying bacteria into a nitrifying bacteria seed culture medium for culturing to obtain nitrifying bacteria;
inoculating nitrobacteria into a fermentation tank for amplified fermentation to obtain a nitrobacteria agent;
inoculating photosynthetic bacteria into a photosynthetic bacteria seed culture medium for culturing to obtain photosynthetic bacteria;
inoculating photosynthetic bacteria into a fermentation tank for amplified fermentation to obtain a photosynthetic bacteria agent;
inoculating yeast into a yeast strain culture medium to culture to obtain yeast;
inoculating saccharomycetes into a fermentation tank for amplified fermentation to obtain a saccharomycete agent;
mixing an aerobic denitrifying bactericide, a nitrifying bactericide, a photosynthetic bactericide and a yeast bactericide, wherein the aerobic denitrifying bactericide is 2-4 parts, the nitrifying bactericide is 2-4 parts, the photosynthetic bactericide is 3-6 parts, and the yeast bactericide is 1-2 parts.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211310135.9A CN115490339A (en) | 2022-10-25 | 2022-10-25 | Preparation method of bacteria-algae composite preparation suitable for rural domestic sewage treatment |
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| CN202211310135.9A CN115490339A (en) | 2022-10-25 | 2022-10-25 | Preparation method of bacteria-algae composite preparation suitable for rural domestic sewage treatment |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109370954A (en) * | 2018-12-07 | 2019-02-22 | 四川清和科技有限公司 | It is a kind of for the composite bacteria agent of sewage treatment, preparation method and application method |
| CN111088179A (en) * | 2019-12-13 | 2020-05-01 | 郑州大学 | Compound microbial agent for ecological restoration and preparation method thereof |
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- 2022-10-25 CN CN202211310135.9A patent/CN115490339A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109370954A (en) * | 2018-12-07 | 2019-02-22 | 四川清和科技有限公司 | It is a kind of for the composite bacteria agent of sewage treatment, preparation method and application method |
| CN111088179A (en) * | 2019-12-13 | 2020-05-01 | 郑州大学 | Compound microbial agent for ecological restoration and preparation method thereof |
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